AT1G54790

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002437
Physical Location & Seq
Reverse (-)
20440979 .. 20444066
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54790.2

Sequence Viewer

Length: 1149 bp
ATGAATATAACTAAGATGAAACTCTTCTATGTGATCCTATTTTTTATCAGTTCTTTACAGATTTCAAACTCCATCGATTTCAATTATCCGTCTGCTTTTAATTTCGGAGATTCCAATTCTGATACCGGTGATCTTGTTGCTGGCCTTGGCATTCGTCTAGACCTTCCTAACGGACAAAATTCCTTCAAAACTTCATCCCAAAGATTCTGCGATGGACGTCTTGTTATAGATTTTCTAATGGATGAAATGGACCTGCCATTTTTAAATCCATATTTGGATTCATTAGGTTTACCAAATTTCAAAAAAGGGTGTAACTTTGCAGCAGCAGGATCCACAATTCTTCCCGCAAACCCGACCTCGGTCTCTCCTTTCTCCTTTGATCTTCAAATCTCTCAATTCATCCGGTTCAAATCTAGGGCCATTGAATTACTTTCTAAAACAGGAAGGAAATATGAAAAGTACTTACCACCAATTGATTATTATTCAAAAGGATTATACATGATTGACATAGGCCAAAATGATATAGCTGGTGCCTTCTACTCCAAGACTCTTGACCAAGTTCTTGCCTCTATTCCTTCCATTCTTGAAACTTTTGAAGCTGGCCTCAAGAGGCTTTATGAGGAAGGAGGCAGAAACATTTGGATACACAACACAGGGCCTTTAGGATGTTTAGCTCAAAATATTGCCAAGTTTGGGACAGATTCAACAAAACTTGATGAGTTTGGTTGCGTTAGTTCCCATAACCAAGCAGCTAAGCTTTTCAATCTTCAACTCCATGCTATGTCCAACAAATTTCAAGCTCAATACCCTGATGCAAATGTTACTTACGTTGACATCTTCTCTATTAAATCTAATCTCATCGCTAATTATTCCCGATTTGGCTTCGAAAAACCGTTAATGGCGTGTTGTGGAGTCGGAGGGGCGCCATTAAATTACGATAGTCGCATTACATGTGGCCAAACTAAAGTCTTAGATGGGATATCAGTAACAGCCAAAGCTTGCAACGATAGTTCCGAGTACATAAACTGGGATGGAATTCATTATACCGAAGCCGCAAACGAGTTCGTCTCATCTCAAATCTTAACCGGAAAATATTCCGATCCACCGTTTTCAGATCAAATGCCATTTTTTCTAACCTTGAAATTTTAA

Protein Analysis

382

Amino Acids

42.41

Weight (kDa)

5.47

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 385 3.7e-42 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 220
Acc36I ACCTGC 1 cut(s) 261
AccB1I GGYRCC 2 cut(s) 530, 922
AciI CCGC 2 cut(s) 345, 1053
AclWI GGATC 4 cut(s) 28, 324, 337, 1094
AcoI YGGCCR 1 cut(s) 955
AcsI RAATTY 5 cut(s) 178, 295, 791, 1035, 1142
AcyI GRCGYC 2 cut(s) 217, 923
AfaI GTAC 2 cut(s) 461, 1019
AfiI CCNNNNNNNGG 2 cut(s) 358, 693
AflIII ACRYGT 1 cut(s) 950
AgeI ACCGGT 1 cut(s) 125
AluBI AGCT 7 cut(s) 527, 599, 674, 752, 757, 800, 998
AluI AGCT 7 cut(s) 527, 599, 674, 752, 757, 800, 998
Alw26I GTCTC 2 cut(s) 367, 1072
AlwI GGATC 4 cut(s) 28, 324, 337, 1094
AoxI GGCC 6 cut(s) 142, 417, 511, 601, 656, 955
ApeKI GCWGC 3 cut(s) 320, 323, 749
ApoI RAATTY 5 cut(s) 178, 295, 791, 1035, 1142
AsiGI ACCGGT 1 cut(s) 125
Asp700I GAANNNNTTC 2 cut(s) 23, 1093
AspLEI GCGC 1 cut(s) 925
AspS9I GGNCC 3 cut(s) 250, 417, 656
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 885
AvaII GGWCC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 957
BamHI GGATCC 1 cut(s) 329
BanI GGYRCC 2 cut(s) 530, 922
BbvI GCAGC 3 cut(s) 332, 335, 761
BccI CCATC 4 cut(s) 80, 206, 968, 1025
BciVI GTATCC 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 367, 1072
BfaI CTAG 2 cut(s) 158, 414
BfoI RGCGCY 1 cut(s) 926
BfuAI ACCTGC 1 cut(s) 261
BfuI GTATCC 1 cut(s) 636
BisI GCNGC 4 cut(s) 321, 324, 750, 1053
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 4 cut(s) 322, 325, 751, 1054
BmcAI AGTACT 1 cut(s) 461
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 250, 417, 656
BmiI GGNNCC 3 cut(s) 331, 532, 924
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 1036
BoxI GACNNNNGTC 1 cut(s) 359
BplI GAGNNNNNCTC 2 cut(s) 1052, 1084
Bpu1102I GCTNAGC 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 217, 923
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 145, 357
BsaWI WCCGGW 3 cut(s) 125, 402, 1085
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 693
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 1031
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 145, 357
BseGI GGATG 5 cut(s) 194, 247, 399, 671, 1036
BseLI CCNNNNNNNGG 2 cut(s) 358, 693
BseNI ACTGG 1 cut(s) 1031
BseXI GCAGC 3 cut(s) 332, 335, 761
BshFI GGCC 6 cut(s) 144, 419, 513, 603, 658, 957
BshNI GGYRCC 2 cut(s) 530, 922
BshTI ACCGGT 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 75
BsiSI CCGG 3 cut(s) 126, 403, 1086
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 2 cut(s) 358, 693
BsmAI GTCTC 2 cut(s) 367, 1072
BsmBI CGTCTC 1 cut(s) 1072
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 6 cut(s) 144, 419, 513, 603, 658, 957
Bso31I GGTCTC 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 885
Bsp143I GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
Bsp1720I GCTNAGC 1 cut(s) 753
BspACI CCGC 2 cut(s) 345, 1053
BspANI GGCC 6 cut(s) 144, 419, 513, 603, 658, 957
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 331, 532, 924
BspMI ACCTGC 1 cut(s) 261
BspPI GGATC 4 cut(s) 28, 324, 337, 1094
BspT104I TTCGAA 1 cut(s) 885
BspT107I GGYRCC 2 cut(s) 530, 922
BspTNI GGTCTC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 1031
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 145, 357
BssMI GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
BssNI GRCGYC 2 cut(s) 217, 923
BssT1I CCWWGG 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 894, 1107
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 2 cut(s) 217, 923
BstBI TTCGAA 1 cut(s) 885
BstC8I GCNNGC 3 cut(s) 142, 601, 1000
BstDEI CTNAG 3 cut(s) 12, 753, 970
BstF5I GGATG 5 cut(s) 194, 247, 399, 671, 1036
BstH2I RGCGCY 1 cut(s) 926
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 6 cut(s) 36, 133, 332, 382, 1102, 1117
BstMAI GTCTC 2 cut(s) 367, 1072
BstMBI GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
BstNSI RCATGY 1 cut(s) 954
BstPAI GACNNNNGTC 1 cut(s) 359
BstV1I GCAGC 3 cut(s) 332, 335, 761
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
Bsu15I ATCGAT 1 cut(s) 75
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 6 cut(s) 144, 419, 513, 603, 658, 957
BsuTUI ATCGAT 1 cut(s) 75
BtgZI GCGATG 2 cut(s) 225, 844
BtsCI GGATG 5 cut(s) 194, 247, 399, 671, 1036
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 3 cut(s) 142, 601, 1000
CfoI GCGC 1 cut(s) 925
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 250, 417, 656
ClaI ATCGAT 1 cut(s) 75
Csp6I GTAC 2 cut(s) 460, 1018
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 3 cut(s) 499, 776, 951
CviQI GTAC 2 cut(s) 460, 1018
DdeI CTNAG 3 cut(s) 12, 753, 970
DinI GGCGCC 1 cut(s) 924
DpnI GATC 6 cut(s) 35, 132, 331, 381, 1101, 1116
DpnII GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
DraI TTTAAA 1 cut(s) 264
EaeI YGGCCR 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 367
Eco32I GATATC 1 cut(s) 981
Eco47I GGWCC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 656
EcoRI GAATTC 1 cut(s) 1035
EcoRV GATATC 1 cut(s) 981
EcoT14I CCWWGG 1 cut(s) 145
EgeI GGCGCC 1 cut(s) 924
EheI GGCGCC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 145
Esp3I CGTCTC 1 cut(s) 1072
FaeI CATG 3 cut(s) 502, 779, 954
FaqI GGGAC 1 cut(s) 709
FatI CATG 3 cut(s) 498, 775, 950
FauI CCCGC 1 cut(s) 352
Fnu4HI GCNGC 4 cut(s) 321, 324, 750, 1053
FokI GGATG 5 cut(s) 181, 254, 386, 678, 1043
Fsp4HI GCNGC 4 cut(s) 321, 324, 750, 1053
FspBI CTAG 2 cut(s) 158, 414
GlaI GCGC 1 cut(s) 924
GluI GCNGC 4 cut(s) 321, 324, 750, 1053
HaeII RGCGCY 1 cut(s) 926
HaeIII GGCC 6 cut(s) 144, 419, 513, 603, 658, 957
HapII CCGG 3 cut(s) 126, 403, 1086
HhaI GCGC 1 cut(s) 925
Hin1I GRCGYC 2 cut(s) 217, 923
Hin1II CATG 3 cut(s) 502, 779, 954
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 2 cut(s) 755, 996
HinfI GANTC 6 cut(s) 110, 204, 278, 547, 701, 912
HpaII CCGG 3 cut(s) 126, 403, 1086
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 290, 832
Hpy188I TCNGA 6 cut(s) 107, 121, 917, 1015, 1099, 1114
Hpy188III TCNNGA 5 cut(s) 158, 551, 584, 607, 873
Hpy8I GTNNAC 2 cut(s) 290, 832
HpyAV CCTTC 6 cut(s) 173, 193, 438, 544, 585, 617
HpyCH4III ACNGT 2 cut(s) 894, 1107
HpyCH4IV ACGT 2 cut(s) 217, 828
HpyCH4V TGCA 3 cut(s) 320, 815, 1002
HpyF3I CTNAG 3 cut(s) 12, 753, 970
HpySE526I ACGT 2 cut(s) 217, 828
Hsp92I GRCGYC 2 cut(s) 217, 923
Hsp92II CATG 3 cut(s) 502, 779, 954
HspAI GCGC 1 cut(s) 923
KasI GGCGCC 1 cut(s) 922
Kzo9I GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
Lsp1109I GCAGC 3 cut(s) 332, 335, 761
LweI GCATC 1 cut(s) 802
MaeI CTAG 2 cut(s) 158, 414
MaeII ACGT 2 cut(s) 217, 828
MaeIII GTNAC 3 cut(s) 311, 820, 985
MalI GATC 6 cut(s) 35, 132, 331, 381, 1101, 1116
MboI GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
MboII GAAGA 5 cut(s) 16, 332, 374, 758, 829
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 957
MluNI TGGCCA 1 cut(s) 957
Mly113I GGCGCC 1 cut(s) 923
MlyI GAGTC 2 cut(s) 541, 921
MmeI TCCRAC 2 cut(s) 810, 895
MnlI CCTC 7 cut(s) 367, 577, 603, 613, 614, 620, 911
Mox20I TGGCCA 1 cut(s) 957
MroXI GAANNNNTTC 2 cut(s) 23, 1093
MscI TGGCCA 1 cut(s) 957
MseI TTAA 7 cut(s) 99, 263, 846, 896, 929, 1082, 1147
Msp20I TGGCCA 1 cut(s) 957
MspI CCGG 3 cut(s) 126, 403, 1086
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 150
NarI GGCGCC 1 cut(s) 923
NdeII GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
NlaIII CATG 3 cut(s) 502, 779, 954
NlaIV GGNNCC 3 cut(s) 331, 532, 924
NspI RCATGY 1 cut(s) 954
NspV TTCGAA 1 cut(s) 885
PciI ACATGT 1 cut(s) 950
PcsI WCGNNNNNNNCGW 1 cut(s) 1011
PctI GAATGC 1 cut(s) 150
PdmI GAANNNNTTC 2 cut(s) 23, 1093
PfeI GAWTC 4 cut(s) 110, 204, 278, 701
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 4 cut(s) 322, 325, 751, 1054
PleI GAGTC 2 cut(s) 541, 920
PluTI GGCGCC 1 cut(s) 926
PpsI GAGTC 2 cut(s) 541, 920
PscI ACATGT 1 cut(s) 950
PshAI GACNNNNGTC 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 331, 532, 924
PspPI GGNCC 3 cut(s) 250, 417, 656
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 2 cut(s) 461, 1019
RsaNI GTAC 2 cut(s) 460, 1018
SaqAI TTAA 7 cut(s) 99, 263, 846, 896, 929, 1082, 1147
SatI GCNGC 4 cut(s) 321, 324, 750, 1053
Sau3AI GATC 6 cut(s) 33, 130, 329, 379, 1099, 1114
Sau96I GGNCC 3 cut(s) 250, 417, 656
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 2 cut(s) 541, 921
SfaNI GCATC 1 cut(s) 802
SfoI GGCGCC 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 885
SinI GGWCC 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SsiI CCGC 2 cut(s) 345, 1053
SspDI GGCGCC 1 cut(s) 922
SspI AATATT 2 cut(s) 682, 1094
SspMI CTAG 2 cut(s) 158, 414
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 894, 1107
TaiI ACGT 2 cut(s) 220, 831
TaqI TCGA 2 cut(s) 75, 885
TaqII GACCGA 1 cut(s) 349
TatI WGTACW 2 cut(s) 459, 1017
TauI GCSGC 1 cut(s) 1055
TfiI GAWTC 4 cut(s) 110, 204, 278, 701
Tru1I TTAA 7 cut(s) 99, 263, 846, 896, 929, 1082, 1147
Tru9I TTAA 7 cut(s) 99, 263, 846, 896, 929, 1082, 1147
TseI GCWGC 3 cut(s) 320, 323, 749
TspDTI ATGAA 8 cut(s) 17, 32, 183, 258, 270, 388, 468, 1028
TspGWI ACGGA 2 cut(s) 78, 186
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 5 cut(s) 178, 295, 791, 1035, 1142
XbaI TCTAGA 1 cut(s) 157
XceI RCATGY 1 cut(s) 954
XmnI GAANNNNTTC 2 cut(s) 23, 1093
XspI CTAG 2 cut(s) 158, 414
ZraI GACGTC 1 cut(s) 218
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.