Rh3BG057200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002437
Physical Location & Seq
Reverse (-)
3911998 .. 3914484
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG057200.1

Sequence Viewer

Length: 1143 bp
ATGGCTTCCAATACCATTCTCCAGATTGTCACCTTACTGTCCATATTTTTGCCTAATGCAAAGTCAGTTCAGTTCAACTATCCTGCCGTTTTCAACTTTGGTGATTCAAACTCCGACACAGGTGAACTTGCTGCTGGCCTTGGTTTTGAGCTCCCACCTCCATATGGACAGACTTACCCCAAACTTTCACCAATCGGAAGGGCTTGTGATGGCCGTCTTATTGTAGACTTTCTAATGGATGCAATGAACCTGCCATTTCTAAATGCCTACTTAGATTCAGTTGGAATGCCGAGTTTCCGAAGAGGGTGTAACTTTGCTGCTGCAGGGTCTACTATACTTCCAGCTTCTCCAACATCTGTGAGCCCATTTTCATTTGCTGTCCAAGTCTCTCAGTTTCTTAGATTCAAAGCCAGGGTTCTTGAAATTCTAGCTGAAGATAAGAAATTTGAAAAGTATCTTCCCACAGAAGATTTTTTTGGGAAGGGGCTTTACATGTTTGACATTGGCCAAAATGACATTGCTGGTGGATTTTACTCTAAGACATTAGAACAGATTCTCGCTTCAATTCCAACAATTCTGGCAGAATTCGAAACAGGAATAAAGAGACTATATGATCAAGGGGCTAGGAACTTTTGGATACACAATACAGGTCCTGTGGGATGCTTGACTCAGAATGTTGCCAAATTCGGAACAGACCCCTCAAAGCTTGATGATCAAGGATGTGTTAGCGCACACAACCAAGCTGCCAAAACTTTTAACCTGCAGCTTCATGCTCTCACCAAAAAGTTGCAAGGCCAATATACTGATGCAAATGTCACATATGTTGATATCTTCTCTATAAAATCTAACCTCATTGCAAACTATTCAAAAAATGGATTCGAACAACCTATTATGGCTTGCTGTGGATATGGAGGTCCACCTTTGAACTATGACAGTCAGATAAGCTGTGGGCAAACGAAGGTCTTGAATGGGAGCTCTGTCACAGCCAAAGGGTGCAGTGAAAGTAGTGCCTATGTTAGTTGGGATGGGATTCATTACACTGAGGCTGCAAATCAGTTTGTCTCATCACAGATACTCACCGGAATATATTCTGATCCACCCTTTTCAGACCAAATGCCTTTCCTTCTGGACCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.48

Weight (kDa)

5.29

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 357 5.8e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 258, 768
AccI GTMKAC 2 cut(s) 225, 329
AclWI GGATC 1 cut(s) 1088
AcoI YGGCCR 2 cut(s) 211, 505
AcsI RAATTY 4 cut(s) 423, 443, 584, 683
AcuI CTGAAG 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 492
AjnI CCWGG 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AloI GAACNNNNNNTCC 1 cut(s) 1121
AluBI AGCT 8 cut(s) 151, 344, 431, 706, 743, 766, 945, 975
AluI AGCT 8 cut(s) 151, 344, 431, 706, 743, 766, 945, 975
Alw21I GWGCWC 2 cut(s) 153, 977
Alw26I GTCTC 3 cut(s) 391, 598, 1066
AlwI GGATC 1 cut(s) 1088
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 4 cut(s) 136, 211, 505, 793
ApeKI GCWGC 6 cut(s) 131, 317, 320, 743, 763, 1046
ApoI RAATTY 4 cut(s) 423, 443, 584, 683
Asp700I GAANNNNTTC 2 cut(s) 552, 1087
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 3 cut(s) 650, 914, 1129
AsuHPI GGTGA 6 cut(s) 22, 113, 134, 180, 769, 1069
AsuII TTCGAA 2 cut(s) 588, 879
AvaII GGWCC 3 cut(s) 650, 914, 1129
BalI TGGCCA 1 cut(s) 507
BanII GRGCYC 3 cut(s) 153, 365, 977
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
Bbv12I GWGCWC 2 cut(s) 153, 977
BbvI GCAGC 6 cut(s) 118, 304, 307, 730, 775, 1033
BccI CCATC 2 cut(s) 203, 1019
BceAI ACGGC 2 cut(s) 71, 198
BciT130I CCWGG 1 cut(s) 412
BciVI GTATCC 1 cut(s) 630
BclI TGATCA 2 cut(s) 613, 712
BcoDI GTCTC 3 cut(s) 391, 598, 1066
BfaI CTAG 2 cut(s) 428, 624
BfmI CTRYAG 2 cut(s) 321, 761
BfuAI ACCTGC 2 cut(s) 258, 768
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 6 cut(s) 132, 318, 321, 744, 764, 1047
BlsI GCNGC 6 cut(s) 133, 319, 322, 745, 765, 1048
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 3 cut(s) 650, 914, 1129
BmgT120I GGNCC 3 cut(s) 650, 914, 1129
BmrFI CCNGG 1 cut(s) 412
BmsI GCATC 3 cut(s) 229, 650, 796
BpmI CTGGAG 1 cut(s) 5
Bpu14I TTCGAA 2 cut(s) 588, 879
BpuEI CTTGAG 1 cut(s) 1118
BsaJI CCNNGG 2 cut(s) 139, 411
BsaWI WCCGGW 1 cut(s) 1079
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse3DI GCAATG 3 cut(s) 249, 516, 852
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 2 cut(s) 139, 411
BseGI GGATG 4 cut(s) 244, 665, 725, 1030
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 3 cut(s) 249, 516, 852
BseMII CTCAG 3 cut(s) 404, 683, 1032
BseXI GCAGC 6 cut(s) 118, 304, 307, 730, 775, 1033
BsgI GTGCAG 1 cut(s) 1015
BshFI GGCC 4 cut(s) 138, 213, 507, 795
BsiHKAI GWGCWC 2 cut(s) 153, 977
BsiSI CCGG 1 cut(s) 1080
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 3 cut(s) 391, 598, 1066
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 4 cut(s) 138, 213, 507, 795
Bsp119I TTCGAA 2 cut(s) 588, 879
Bsp1286I GDGCHC 3 cut(s) 153, 365, 977
Bsp143I GATC 3 cut(s) 613, 712, 1093
BspANI GGCC 4 cut(s) 138, 213, 507, 795
BspCNI CTCAG 3 cut(s) 403, 682, 1033
BspMAI CTGCAG 2 cut(s) 325, 765
BspMI ACCTGC 2 cut(s) 258, 768
BspPI GGATC 1 cut(s) 1088
BspT104I TTCGAA 2 cut(s) 588, 879
BsrDI GCAATG 3 cut(s) 249, 516, 852
BssECI CCNNGG 2 cut(s) 139, 411
BssMI GATC 3 cut(s) 613, 712, 1093
BssT1I CCWWGG 1 cut(s) 139
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 39, 935
Bst6I CTCTTC 1 cut(s) 295
BstBI TTCGAA 2 cut(s) 588, 879
BstC8I GCNNGC 2 cut(s) 136, 898
BstDEI CTNAG 6 cut(s) 271, 390, 398, 537, 669, 1041
BstF5I GGATG 4 cut(s) 244, 665, 725, 1030
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 3 cut(s) 616, 715, 1096
BstMAI GTCTC 3 cut(s) 391, 598, 1066
BstMBI GATC 3 cut(s) 613, 712, 1093
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 2 cut(s) 321, 761
BstV1I GCAGC 6 cut(s) 118, 304, 307, 730, 775, 1033
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 4 cut(s) 138, 213, 507, 795
BtsCI GGATG 4 cut(s) 244, 665, 725, 1030
BtsI GCAGTG 1 cut(s) 1003
BtsIMutI CAGTG 2 cut(s) 1003, 1038
BveI ACCTGC 2 cut(s) 258, 768
Cac8I GCNNGC 2 cut(s) 136, 898
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 731
Cfr13I GGNCC 3 cut(s) 650, 914, 1129
CviAII CATG 2 cut(s) 493, 770
DdeI CTNAG 6 cut(s) 271, 390, 398, 537, 669, 1041
DpnI GATC 3 cut(s) 615, 714, 1095
DpnII GATC 3 cut(s) 613, 712, 1093
EaeI YGGCCR 2 cut(s) 211, 505
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Ecl136II GAGCTC 2 cut(s) 151, 975
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 3 cut(s) 153, 365, 977
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 3 cut(s) 650, 914, 1129
Eco53kI GAGCTC 2 cut(s) 151, 975
Eco57I CTGAAG 1 cut(s) 453
EcoICRI GAGCTC 2 cut(s) 151, 975
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 3 cut(s) 153, 365, 977
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 496, 773
FatI CATG 2 cut(s) 492, 769
FauNDI CATATG 2 cut(s) 163, 820
FbaI TGATCA 2 cut(s) 613, 712
FblI GTMKAC 2 cut(s) 225, 329
Fnu4HI GCNGC 6 cut(s) 132, 318, 321, 744, 764, 1047
FokI GGATG 4 cut(s) 251, 672, 732, 1037
FriOI GRGCYC 3 cut(s) 153, 365, 977
Fsp4HI GCNGC 6 cut(s) 132, 318, 321, 744, 764, 1047
FspBI CTAG 2 cut(s) 428, 624
GlaI GCGC 1 cut(s) 730
GluI GCNGC 6 cut(s) 132, 318, 321, 744, 764, 1047
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 4 cut(s) 138, 213, 507, 795
HapII CCGG 1 cut(s) 1080
HhaI GCGC 1 cut(s) 731
Hin1II CATG 2 cut(s) 496, 773
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 7 cut(s) 104, 275, 402, 553, 667, 876, 1030
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 6 cut(s) 22, 113, 134, 180, 769, 1069
Hpy166II GTNNAC 4 cut(s) 125, 226, 330, 917
Hpy188I TCNGA 9 cut(s) 115, 197, 299, 672, 689, 939, 1093, 1108, 1142
Hpy188III TCNNGA 4 cut(s) 22, 419, 964, 1127
Hpy8I GTNNAC 4 cut(s) 125, 226, 330, 917
HpyAV CCTTC 4 cut(s) 192, 475, 952, 1133
HpyCH4III ACNGT 2 cut(s) 39, 935
HpyCH4V TGCA 9 cut(s) 59, 242, 323, 763, 790, 809, 857, 996, 1049
HpyF3I CTNAG 6 cut(s) 271, 390, 398, 537, 669, 1041
Hsp92II CATG 2 cut(s) 496, 773
HspAI GCGC 1 cut(s) 729
Ksp22I TGATCA 2 cut(s) 613, 712
Kzo9I GATC 3 cut(s) 613, 712, 1093
LmnI GCTCC 2 cut(s) 156, 972
Lsp1109I GCAGC 6 cut(s) 118, 304, 307, 730, 775, 1033
LweI GCATC 3 cut(s) 229, 650, 796
MaeI CTAG 2 cut(s) 428, 624
MaeIII GTNAC 4 cut(s) 28, 308, 814, 979
MalI GATC 3 cut(s) 615, 714, 1095
MboI GATC 3 cut(s) 613, 712, 1093
MboII GAAGA 5 cut(s) 312, 446, 449, 479, 823
MhlI GDGCHC 3 cut(s) 153, 365, 977
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 6 cut(s) 423, 443, 564, 573, 584, 683
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 1 cut(s) 661
MmeI TCCRAC 4 cut(s) 138, 262, 374, 593
MnlI CCTC 7 cut(s) 168, 296, 709, 860, 905, 1036, 1142
Mox20I TGGCCA 1 cut(s) 507
MroXI GAANNNNTTC 2 cut(s) 552, 1087
MscI TGGCCA 1 cut(s) 507
MseI TTAA 1 cut(s) 756
Msp20I TGGCCA 1 cut(s) 507
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 1 cut(s) 412
Mva1269I GAATGC 1 cut(s) 291
MvaI CCWGG 1 cut(s) 412
NdeI CATATG 2 cut(s) 163, 820
NdeII GATC 3 cut(s) 613, 712, 1093
NlaIII CATG 2 cut(s) 496, 773
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 3 cut(s) 28, 814, 979
NspI RCATGY 1 cut(s) 496
NspV TTCGAA 2 cut(s) 588, 879
PciI ACATGT 1 cut(s) 492
PctI GAATGC 1 cut(s) 291
PdmI GAANNNNTTC 2 cut(s) 552, 1087
PfeI GAWTC 6 cut(s) 104, 275, 402, 553, 876, 1030
PkrI GCNGC 6 cut(s) 133, 319, 322, 745, 765, 1048
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
PpuMI RGGWCCY 1 cut(s) 650
PscI ACATGT 1 cut(s) 492
Psp124BI GAGCTC 2 cut(s) 153, 977
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspPI GGNCC 3 cut(s) 650, 914, 1129
PspPPI RGGWCCY 1 cut(s) 650
PstI CTGCAG 2 cut(s) 325, 765
PstNI CAGNNNCTG 1 cut(s) 653
SacI GAGCTC 2 cut(s) 153, 977
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 6 cut(s) 132, 318, 321, 744, 764, 1047
Sau3AI GATC 3 cut(s) 613, 712, 1093
Sau96I GGNCC 3 cut(s) 650, 914, 1129
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 3 cut(s) 153, 365, 977
SfaNI GCATC 3 cut(s) 229, 650, 796
SfcI CTRYAG 2 cut(s) 321, 761
SfuI TTCGAA 2 cut(s) 588, 879
SinI GGWCC 3 cut(s) 650, 914, 1129
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
Sse9I AATT 6 cut(s) 423, 443, 564, 573, 584, 683
SspMI CTAG 2 cut(s) 428, 624
SstI GAGCTC 2 cut(s) 153, 977
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 39, 935
TaqI TCGA 2 cut(s) 588, 879
TasI AATT 6 cut(s) 423, 443, 564, 573, 584, 683
TfiI GAWTC 6 cut(s) 104, 275, 402, 553, 876, 1030
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 2 cut(s) 1003, 1045
TseFI GTSAC 3 cut(s) 28, 814, 979
TseI GCWGC 6 cut(s) 131, 317, 320, 743, 763, 1046
Tsp45I GTSAC 3 cut(s) 28, 814, 979
TspDTI ATGAA 4 cut(s) 260, 360, 758, 1022
TspRI CASTG 2 cut(s) 1003, 1045
VpaK11BI GGWCC 3 cut(s) 650, 914, 1129
XapI RAATTY 4 cut(s) 423, 443, 584, 683
XceI RCATGY 1 cut(s) 496
XmiI GTMKAC 2 cut(s) 225, 329
XmnI GAANNNNTTC 2 cut(s) 552, 1087
XspI CTAG 2 cut(s) 428, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.