pycom12g20930

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002437
Physical Location & Seq
Forward (+)
22264100 .. 22264372
273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20930.1

Sequence Viewer

Length: 273 bp
ATGGCTGCCAAGAACTCCATTATTCTCCAAGTTTTTGCCTTCCTGATGATTGTCTTACCGGTTGCGAATTCGATAAAGTTCAGCTATCCGGCGGTTTTTAACTTTGGGGATTCGAATTCCGACACCGGCGAGCTCGGTGCCGGGCTCGGTTTCAATCTTCCACTGCCTTATGGACAGACCTACTTCAAACCTCCGTCGTCATCGGGGAGGCCTTGCGATGGCCGTCTGATCGTAGATTTTCTAAGTAAGTCTCTGACCTTATTACTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

91

Amino Acids

9.66

Weight (kDa)

7.87

Isoelectric Point (pI)

27.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AciI CCGC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 2 cut(s) 67, 115
AfiI CCNNNNNNNGG 1 cut(s) 218
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 2 cut(s) 154, 187
AluBI AGCT 2 cut(s) 84, 133
AluI AGCT 2 cut(s) 84, 133
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 255
AoxI GGCC 2 cut(s) 209, 220
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 67, 115
AsiGI ACCGGT 1 cut(s) 58
AsuC2I CCSGG 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 113
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 2 cut(s) 135, 147
Bbv12I GWGCWC 1 cut(s) 135
BccI CCATC 1 cut(s) 212
BceAI ACGGC 1 cut(s) 207
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 255
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse118I RCCGGY 2 cut(s) 58, 125
BseLI CCNNNNNNNGG 1 cut(s) 218
BshFI GGCC 2 cut(s) 211, 222
BshNI GGYRCC 1 cut(s) 137
BshTI ACCGGT 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 4 cut(s) 59, 89, 126, 141
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 255
BsnI GGCC 2 cut(s) 211, 222
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 135, 147
Bsp143I GATC 1 cut(s) 228
BspACI CCGC 1 cut(s) 92
BspANI GGCC 2 cut(s) 211, 222
BspLI GGNNCC 1 cut(s) 139
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 137
BsrFI RCCGGY 2 cut(s) 58, 125
BssAI RCCGGY 2 cut(s) 58, 125
BssMI GATC 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 242
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 211, 222
BtgZI GCGATG 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 131
Cfr10I RCCGGY 2 cut(s) 58, 125
CspAI ACCGGT 1 cut(s) 58
CviJI RGCY 6 cut(s) 5, 84, 133, 145, 211, 222
CviKI_1 RGCY 6 cut(s) 5, 84, 133, 145, 211, 222
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 133
Eco147I AGGCCT 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 135, 147
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 2 cut(s) 67, 115
EcoT38I GRGCYC 2 cut(s) 135, 147
FaiI YATR 2 cut(s) 171, 271
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 2 cut(s) 135, 147
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 211, 222
HapII CCGG 4 cut(s) 59, 89, 126, 141
HinfI GANTC 1 cut(s) 110
HpaII CCGG 4 cut(s) 59, 89, 126, 141
Hpy188I TCNGA 3 cut(s) 121, 228, 255
Hpy188III TCNNGA 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 49
HpyF3I CTNAG 1 cut(s) 242
Kzo9I GATC 1 cut(s) 228
LpnPI CCDG 5 cut(s) 56, 72, 102, 139, 154
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 1 cut(s) 149
MhlI GDGCHC 2 cut(s) 135, 147
MluCI AATT 2 cut(s) 67, 115
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 2 cut(s) 201, 201
MseI TTAA 1 cut(s) 99
MspI CCGG 4 cut(s) 59, 89, 126, 141
MspR9I CCNGG 1 cut(s) 142
NciI CCSGG 1 cut(s) 142
NdeII GATC 1 cut(s) 228
NlaIV GGNNCC 1 cut(s) 139
NspV TTCGAA 1 cut(s) 113
PceI AGGCCT 1 cut(s) 211
PfeI GAWTC 1 cut(s) 110
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 139
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 2 cut(s) 135, 147
SetI ASST 5 cut(s) 86, 135, 182, 193, 260
SfuI TTCGAA 1 cut(s) 113
SgrAI CRCCGGYG 1 cut(s) 125
Sse9I AATT 2 cut(s) 67, 115
SseBI AGGCCT 1 cut(s) 211
SsiI CCGC 1 cut(s) 92
SstI GAGCTC 1 cut(s) 135
StuI AGGCCT 1 cut(s) 211
StyD4I CCNGG 1 cut(s) 140
TaqI TCGA 2 cut(s) 71, 113
TasI AATT 2 cut(s) 67, 115
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 168
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 1 cut(s) 168
XapI RAATTY 2 cut(s) 67, 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.