Rw3G004280

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002437
Physical Location & Seq
Reverse (-)
3732633 .. 3735121
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004280.1

Sequence Viewer

Length: 1407 bp
ATGGCTTCCAATACCATTCTCCAGATTGTCACCTTACTGTCCATATTTTTGCCTAATGCCAAGTCAGTTCAGTTCAACTATCCTGCCGTTTTCAACTTTGGTGATTCAAACTCCGACACAGGTGAACTTGCTGCTGGCCTTGGTTTCGAGCTCTCACCTCCATATGGACAGACTTACCCCAAACTTTCACCAATTGGAAGGGCTTGTGATGGCCGTCTTATCGTAGACTTTCTAATCACCATGAGGGTGTCCTGTCTTGCATTATTTGCCATGGCTGCCAAGACCTGCATTCTCCAAATCTCCATCTTCTTCCTATTACCTCTTGCCAATTCTATCCACTTCAAGTATCCAACTGTCTTCAACTTTGGTGACTCAAATTCCGACACCGGAAACCTTGTGTCCGCCGGCATTGAGACCCTTGCCCCTCCCAATGGCCAGAGCTACTTCAAAACCCCATCTGGCAGATACTGTGATGGCCGTCTCATCGTCGATTTTCTTATGGATGCAATGAACCTGCCATTTCTAAATGCCTACTTAGATTCAGTTGGAGTGCCGAGTTTCCAAAGAGGGTGTAACTTTGCTGCTGCAGGGTCTACTATACTTCCAGCTTCTCCAACATCTGTGAGCCCATTTTCATTTGCTGTCCAAGTCTCTCAGTTTCTTAGATTCAAAGCCAGGGTTCTTGAAATTCTAGCTAAAGGTAAGAAATTTGAAAAGTATCTTCCCACAGAAGATTTTTTTGGGAAGGGGCTTTACATGTTTGACATTGGCCAAAATGACATTGCTGGTGGATTTTACTCTAAGACATTAGAACAGATTCTCGCTTCAATTCCAACAATTCTGGCAGAATTCGAAACAGGAATAAAGAGACTATATGATCAAGGGGCTAGGAACTTTTGGATACACAATACAGGTCCTGTGGGATGCTTGACTCAGAATGTTGCCAAATTCGGAACAGACCCCTCTAAGCTTGATGATCAAGGATGCGTTAGAGCACACAACCAAGCTGCCAAAACTTTTAACCTGCAGCTTCATGCTCTCACCAAAAAGTTGCAAGGCCAATATACTGATGCAAATGTCACATATGTTGATATCTTCTCTATAAAATCTAACCTCATTGCAAACTATTCAAAAAATGGATTCGAACAACCTATTATGGCTTGCTGTGGATATGGAGGTCCACCTTTGAACTATGACAGTCAGATAAGCTGTGGGCAAACGAAGGTCTTGAATGGGAGCTCTGTCACAGCCAAAGGGTGCAGTGAAAGTAGTGCCTATGTTAGTTGGGATGGGATTCATTACACTGAGGCTGCAAATCAGTTTGTCTCATCACAGATACTCACCGGAATATATTCTGATCCACCCTTTTCAGACCAAATGCCTTTCCTTCTGGACCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

468

Amino Acids

51.01

Weight (kDa)

6.23

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 119 - 445 4.8e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 293, 522, 1032
AccI GTMKAC 2 cut(s) 225, 593
AciI CCGC 1 cut(s) 402
AclWI GGATC 1 cut(s) 1352
AcoI YGGCCR 4 cut(s) 211, 433, 475, 769
AcsI RAATTY 5 cut(s) 376, 687, 707, 848, 947
AfiI CCNNNNNNNGG 2 cut(s) 164, 431
AflIII ACRYGT 1 cut(s) 756
AjnI CCWGG 1 cut(s) 674
AjuI GAANNNNNNNTTGG 2 cut(s) 723, 755
AloI GAACNNNNNNTCC 1 cut(s) 1385
AluBI AGCT 9 cut(s) 151, 441, 608, 695, 970, 1007, 1030, 1209, 1239
AluI AGCT 9 cut(s) 151, 441, 608, 695, 970, 1007, 1030, 1209, 1239
Alw21I GWGCWC 3 cut(s) 153, 997, 1241
Alw26I GTCTC 5 cut(s) 407, 485, 655, 862, 1330
AlwI GGATC 1 cut(s) 1352
AlwNI CAGNNNCTG 2 cut(s) 468, 917
AoxI GGCC 6 cut(s) 136, 211, 433, 475, 769, 1057
ApeKI GCWGC 7 cut(s) 131, 275, 581, 584, 1007, 1027, 1310
ApoI RAATTY 5 cut(s) 376, 687, 707, 848, 947
Asp700I GAANNNNTTC 2 cut(s) 816, 1351
AspS9I GGNCC 3 cut(s) 914, 1178, 1393
AsuHPI GGTGA 9 cut(s) 22, 113, 134, 147, 180, 229, 380, 1033, 1333
AsuII TTCGAA 2 cut(s) 852, 1143
AvaII GGWCC 3 cut(s) 914, 1178, 1393
BalI TGGCCA 2 cut(s) 435, 771
BanII GRGCYC 3 cut(s) 153, 629, 1241
BarI GAAGNNNNNNTAC 2 cut(s) 737, 769
BbsI GAAGAC 1 cut(s) 349
Bbv12I GWGCWC 3 cut(s) 153, 997, 1241
BbvI GCAGC 7 cut(s) 118, 262, 568, 571, 994, 1039, 1297
BccI CCATC 5 cut(s) 203, 311, 463, 467, 1283
BceAI ACGGC 3 cut(s) 71, 198, 462
BciT130I CCWGG 1 cut(s) 676
BciVI GTATCC 2 cut(s) 357, 894
BclI TGATCA 2 cut(s) 877, 976
BcoDI GTCTC 5 cut(s) 407, 485, 655, 862, 1330
BfaI CTAG 2 cut(s) 692, 888
BfmI CTRYAG 2 cut(s) 585, 1025
BfuAI ACCTGC 3 cut(s) 293, 522, 1032
BfuI GTATCC 2 cut(s) 357, 894
BisI GCNGC 7 cut(s) 132, 276, 582, 585, 1008, 1028, 1311
BlsI GCNGC 7 cut(s) 133, 277, 583, 586, 1009, 1029, 1312
Bme1390I CCNGG 1 cut(s) 676
Bme18I GGWCC 3 cut(s) 914, 1178, 1393
BmgT120I GGNCC 3 cut(s) 914, 1178, 1393
BmrFI CCNGG 1 cut(s) 676
BmsI GCATC 4 cut(s) 493, 914, 974, 1060
BpiI GAAGAC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 5
Bpu14I TTCGAA 2 cut(s) 852, 1143
BpuEI CTTGAG 1 cut(s) 1382
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 139, 270, 675
BsaWI WCCGGW 2 cut(s) 386, 1343
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 431
Bse118I RCCGGY 1 cut(s) 404
Bse3DI GCAATG 3 cut(s) 513, 780, 1116
BseBI CCWGG 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 139, 270, 675
BseGI GGATG 4 cut(s) 508, 929, 989, 1294
BseLI CCNNNNNNNGG 2 cut(s) 164, 431
BseMI GCAATG 3 cut(s) 513, 780, 1116
BseMII CTCAG 3 cut(s) 668, 947, 1296
BseXI GCAGC 7 cut(s) 118, 262, 568, 571, 994, 1039, 1297
BsgI GTGCAG 1 cut(s) 1279
BshFI GGCC 6 cut(s) 138, 213, 435, 477, 771, 1059
BsiHKAI GWGCWC 3 cut(s) 153, 997, 1241
BsiSI CCGG 3 cut(s) 387, 405, 1344
BslI CCNNNNNNNGG 2 cut(s) 164, 431
BsmAI GTCTC 5 cut(s) 407, 485, 655, 862, 1330
BsmBI CGTCTC 1 cut(s) 485
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 6 cut(s) 138, 213, 435, 477, 771, 1059
Bso31I GGTCTC 1 cut(s) 407
Bsp119I TTCGAA 2 cut(s) 852, 1143
Bsp1286I GDGCHC 4 cut(s) 153, 629, 997, 1241
Bsp143I GATC 3 cut(s) 877, 976, 1357
Bsp19I CCATGG 1 cut(s) 270
BspACI CCGC 1 cut(s) 402
BspANI GGCC 6 cut(s) 138, 213, 435, 477, 771, 1059
BspCNI CTCAG 3 cut(s) 667, 946, 1297
BspMAI CTGCAG 2 cut(s) 589, 1029
BspMI ACCTGC 3 cut(s) 293, 522, 1032
BspPI GGATC 1 cut(s) 1352
BspT104I TTCGAA 2 cut(s) 852, 1143
BspTNI GGTCTC 1 cut(s) 407
BsrDI GCAATG 3 cut(s) 513, 780, 1116
BsrFI RCCGGY 1 cut(s) 404
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 3 cut(s) 139, 270, 675
BssMI GATC 3 cut(s) 877, 976, 1357
BssT1I CCWWGG 2 cut(s) 139, 270
Bst2UI CCWGG 1 cut(s) 676
Bst4CI ACNGT 4 cut(s) 39, 355, 470, 1199
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBI TTCGAA 2 cut(s) 852, 1143
BstC8I GCNNGC 3 cut(s) 136, 406, 1162
BstDEI CTNAG 7 cut(s) 535, 654, 662, 801, 933, 966, 1305
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 4 cut(s) 508, 929, 989, 1294
BstKTI GATC 3 cut(s) 880, 979, 1360
BstMAI GTCTC 5 cut(s) 407, 485, 655, 862, 1330
BstMBI GATC 3 cut(s) 877, 976, 1357
BstMWI GCNNNNNNNGC 2 cut(s) 266, 275
BstNI CCWGG 1 cut(s) 676
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 674
BstSFI CTRYAG 2 cut(s) 585, 1025
BstV1I GCAGC 7 cut(s) 118, 262, 568, 571, 994, 1039, 1297
BstV2I GAAGAC 1 cut(s) 349
BsuI GTATCC 2 cut(s) 357, 894
BsuRI GGCC 6 cut(s) 138, 213, 435, 477, 771, 1059
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 4 cut(s) 508, 929, 989, 1294
BtsI GCAGTG 1 cut(s) 1267
BtsIMutI CAGTG 2 cut(s) 1267, 1302
BveI ACCTGC 3 cut(s) 293, 522, 1032
Cac8I GCNNGC 3 cut(s) 136, 406, 1162
CaiI CAGNNNCTG 2 cut(s) 468, 917
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 914, 1178, 1393
CviAII CATG 4 cut(s) 241, 271, 757, 1034
DdeI CTNAG 7 cut(s) 535, 654, 662, 801, 933, 966, 1305
DpnI GATC 3 cut(s) 879, 978, 1359
DpnII GATC 3 cut(s) 877, 976, 1357
EaeI YGGCCR 4 cut(s) 211, 433, 475, 769
EciI GGCGGA 1 cut(s) 391
Ecl136II GAGCTC 2 cut(s) 151, 1239
Eco130I CCWWGG 2 cut(s) 139, 270
Eco24I GRGCYC 3 cut(s) 153, 629, 1241
Eco31I GGTCTC 1 cut(s) 407
Eco32I GATATC 1 cut(s) 1093
Eco47I GGWCC 3 cut(s) 914, 1178, 1393
Eco53kI GAGCTC 2 cut(s) 151, 1239
EcoICRI GAGCTC 2 cut(s) 151, 1239
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 848
EcoRII CCWGG 1 cut(s) 674
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 2 cut(s) 139, 270
EcoT38I GRGCYC 3 cut(s) 153, 629, 1241
ErhI CCWWGG 2 cut(s) 139, 270
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 4 cut(s) 244, 274, 760, 1037
FatI CATG 4 cut(s) 240, 270, 756, 1033
FauNDI CATATG 2 cut(s) 163, 1084
FbaI TGATCA 2 cut(s) 877, 976
FblI GTMKAC 2 cut(s) 225, 593
Fnu4HI GCNGC 7 cut(s) 132, 276, 582, 585, 1008, 1028, 1311
FokI GGATG 4 cut(s) 515, 936, 996, 1301
FriOI GRGCYC 3 cut(s) 153, 629, 1241
Fsp4HI GCNGC 7 cut(s) 132, 276, 582, 585, 1008, 1028, 1311
FspBI CTAG 2 cut(s) 692, 888
GluI GCNGC 7 cut(s) 132, 276, 582, 585, 1008, 1028, 1311
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 6 cut(s) 138, 213, 435, 477, 771, 1059
HapII CCGG 3 cut(s) 387, 405, 1344
Hin1II CATG 4 cut(s) 244, 274, 760, 1037
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 8 cut(s) 104, 371, 539, 666, 817, 931, 1140, 1294
HpaII CCGG 3 cut(s) 387, 405, 1344
HphI GGTGA 9 cut(s) 22, 113, 134, 147, 180, 229, 380, 1033, 1333
Hpy166II GTNNAC 4 cut(s) 125, 226, 594, 1181
Hpy188I TCNGA 8 cut(s) 115, 382, 936, 953, 1203, 1357, 1372, 1406
Hpy188III TCNNGA 4 cut(s) 22, 683, 1228, 1391
Hpy8I GTNNAC 4 cut(s) 125, 226, 594, 1181
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 4 cut(s) 192, 739, 1216, 1397
HpyCH4III ACNGT 4 cut(s) 39, 355, 470, 1199
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 275
HpyF3I CTNAG 7 cut(s) 535, 654, 662, 801, 933, 966, 1305
Hsp92II CATG 4 cut(s) 244, 274, 760, 1037
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 877, 976
Kzo9I GATC 3 cut(s) 877, 976, 1357
LmnI GCTCC 1 cut(s) 1236
Lsp1109I GCAGC 7 cut(s) 118, 262, 568, 571, 994, 1039, 1297
LweI GCATC 4 cut(s) 493, 914, 974, 1060
MaeI CTAG 2 cut(s) 692, 888
MaeIII GTNAC 5 cut(s) 28, 368, 572, 1078, 1243
MalI GATC 3 cut(s) 879, 978, 1359
MboI GATC 3 cut(s) 877, 976, 1357
MboII GAAGA 6 cut(s) 298, 301, 349, 713, 743, 1087
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 4 cut(s) 153, 629, 997, 1241
MlsI TGGCCA 2 cut(s) 435, 771
MluCI AATT 9 cut(s) 192, 328, 376, 687, 707, 828, 837, 848, 947
MluNI TGGCCA 2 cut(s) 435, 771
MlyI GAGTC 2 cut(s) 365, 925
MmeI TCCRAC 6 cut(s) 138, 374, 405, 526, 638, 857
Mox20I TGGCCA 2 cut(s) 435, 771
MroNI GCCGGC 1 cut(s) 404
MroXI GAANNNNTTC 2 cut(s) 816, 1351
MscI TGGCCA 2 cut(s) 435, 771
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 1 cut(s) 245
Msp20I TGGCCA 2 cut(s) 435, 771
MspI CCGG 3 cut(s) 387, 405, 1344
MspR9I CCNGG 1 cut(s) 676
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 1 cut(s) 676
MwoI GCNNNNNNNGC 2 cut(s) 266, 275
NaeI GCCGGC 1 cut(s) 406
NcoI CCATGG 1 cut(s) 270
NdeI CATATG 2 cut(s) 163, 1084
NdeII GATC 3 cut(s) 877, 976, 1357
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 4 cut(s) 244, 274, 760, 1037
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 4 cut(s) 28, 368, 1078, 1243
NspI RCATGY 1 cut(s) 760
NspV TTCGAA 2 cut(s) 852, 1143
PciI ACATGT 1 cut(s) 756
PctI GAATGC 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 816, 1351
PfeI GAWTC 6 cut(s) 104, 539, 666, 817, 1140, 1294
PkrI GCNGC 7 cut(s) 133, 277, 583, 586, 1009, 1029, 1312
PleI GAGTC 2 cut(s) 365, 925
PpsI GAGTC 2 cut(s) 365, 925
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 1 cut(s) 756
Psp124BI GAGCTC 2 cut(s) 153, 1241
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 674
PspPI GGNCC 3 cut(s) 914, 1178, 1393
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 2 cut(s) 589, 1029
PstNI CAGNNNCTG 2 cut(s) 468, 917
RseI CAYNNNNRTG 1 cut(s) 245
SacI GAGCTC 2 cut(s) 153, 1241
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 7 cut(s) 132, 276, 582, 585, 1008, 1028, 1311
Sau3AI GATC 3 cut(s) 877, 976, 1357
Sau96I GGNCC 3 cut(s) 914, 1178, 1393
SchI GAGTC 2 cut(s) 365, 925
ScrFI CCNGG 1 cut(s) 676
SduI GDGCHC 4 cut(s) 153, 629, 997, 1241
SfaNI GCATC 4 cut(s) 493, 914, 974, 1060
SfcI CTRYAG 2 cut(s) 585, 1025
SfuI TTCGAA 2 cut(s) 852, 1143
SinI GGWCC 3 cut(s) 914, 1178, 1393
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 1397
SmoI CTYRAG 1 cut(s) 1397
Sse9I AATT 9 cut(s) 192, 328, 376, 687, 707, 828, 837, 848, 947
SsiI CCGC 1 cut(s) 402
SspMI CTAG 2 cut(s) 692, 888
SstI GAGCTC 2 cut(s) 153, 1241
StyD4I CCNGG 1 cut(s) 674
StyI CCWWGG 2 cut(s) 139, 270
TaaI ACNGT 4 cut(s) 39, 355, 470, 1199
TaqI TCGA 4 cut(s) 147, 489, 852, 1143
TasI AATT 9 cut(s) 192, 328, 376, 687, 707, 828, 837, 848, 947
TfiI GAWTC 6 cut(s) 104, 539, 666, 817, 1140, 1294
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 2 cut(s) 1267, 1309
TseFI GTSAC 4 cut(s) 28, 368, 1078, 1243
TseI GCWGC 7 cut(s) 131, 275, 581, 584, 1007, 1027, 1310
Tsp45I GTSAC 4 cut(s) 28, 368, 1078, 1243
TspDTI ATGAA 4 cut(s) 524, 624, 1022, 1286
TspRI CASTG 2 cut(s) 1267, 1309
VpaK11BI GGWCC 3 cut(s) 914, 1178, 1393
XapI RAATTY 5 cut(s) 376, 687, 707, 848, 947
XceI RCATGY 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 225, 593
XmnI GAANNNNTTC 2 cut(s) 816, 1351
XspI CTAG 2 cut(s) 692, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.