MD12G1223900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002437
Physical Location & Seq
Forward (+)
30033829 .. 30037433
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1223900.v1.1.491

Sequence Viewer

Length: 1674 bp
ATGGCTGCCAAGAACTCCATTATTCTCCAAGTTTTTGCCTTCCTGGTGATTGTCTTACCGGTTGCGAATTCGATAAAGTTCAGCTATCCGGCGGTTTTTAACTTTGGGGATTCAAATTCCGACACCGGCGAGCTCGGTGCCGGGCTCGGTTTCAATCTTCCACTGCCTTATGGACAGACCTACTTCAATCCTCCGTCGTCATCGGGAAGGTCTTGCGATGGCCGTCTGATCGTAGATTTTCTAACCTCTTCATCCATGGCCACCAAAATTTTCACCATCCAAATTCTCACCTTACTTTCCATTTTTCCACCACCTTCAAACTCAACGGAGTTCCGCTACCCGGCCGCTTTCAACTTCGGCGATTCAAACTCTGGCACCGGCGTGCTTGCTGCCGGATTTGATTTCAAGCTCCCACCACCTTATGGACAGTCTTTCTTCAAAACTCCCTCCGGGAGGTTTTGTGATGGCCGTCTCCTCATAGATTTTCTCACAACAGCCGAAGAAGCGAGAGAACTTCCTGTTGCATTTTCCATGGCTACCAATATCTGCATTATCCAAATTCTCACATTGATTTCCATCTCCTTGCCTCTTTCCAAATCCATCCACTTCAACCACCCTGCCGTTTTCAACTTTGGCGATTCGAATTCCGACACTGGCAACCTTGTTTCCGCAGGCATTGAGACCATTATTTCTCCTTATGGACAATCTTATTTCAAAACCCCATCTGGGAGATACTCCGATGGCCGCCTCATCGTCGATTTTCTTATGGATGCAATGGAGCTTCCGTTTCTCAATGCCTACTTGGATTCAGTTGGCCTGCCGAGTTTCCAAAAAGGTTGCAACTTTGCCGCGGCGGGGTCAACTATACTTCCTGCAACTGCAACATCGATCAGCCCATTTTCTTTCGGGGTCCAGGTCTCCCAGTTTTTTAGATTCAAAGCCAAGGTTCTTGAGTTGCTAGCTAAAAAAGAAAGCTCTCCCAAGTATCTGCCCACGGAAGATTTTTTCGCGAAAGGGCTCTACATGTTCGATATAGGCCAGAATGATCTCGCCGGTGCATTTTATTCGAAATCATTGGATGAGATTCTCGCTTCTATTCCAATGATTTTGACAGAATTCGAAACTGGAGTAAAGAGATTATATGACCAAGGAGGTAGGAACTTTTGGATACACAACACAGGTCCTCTCGGATGCTTGACGCAGAATGTTGCCAAGTTTGGAACTGATCCCTCGAAGTTTGACGAACAAGGATGCGTCAGCAACCACAACCAAGCTGCTAAACTCTTTAACCTGCAGCTTCATGCTCTCACCAAAAAGTTGCAAGGCCAGTACTCGAATGCAAGTGTCATATATGTTGATATCTTTTCGATCAAATCTGAGCTCATTTCAAATTATTCGAAAAATGGATTTGAGCAACCTATTATGGCTTGCTGCGGTTACGGAGGTCCCCCGTTGAACTACGACAGTCACATTACCTGTGGACAAACAAAGACGTTGAATGGAACAACTGTCACAGCCAAAGGATGCACAGATAGTTCTGATTACATTAGTTGGGATGGAATTCATTACACCGAAGCTGCGAATCAGTATGTCTCGTCCAAGATACTCACCGGGAAGTATTCTGATCCACCCTTCTCGGATAAAATGCCTTTCCTTCTGAAGCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04728 map04144 map04728
Pfam Domains
Protein Families
Kinesin Lipase_GDSL

Protein Analysis

558

Amino Acids

60.72

Weight (kDa)

6.02

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 208 - 534 2.3e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1299
AccB1I GGYRCC 2 cut(s) 137, 374
AccB7I CCANNNNNTGG 1 cut(s) 422
AccII CGCG 2 cut(s) 851, 1010
AciI CCGC 9 cut(s) 92, 334, 345, 669, 745, 849, 851, 854, 1434
AclWI GGATC 2 cut(s) 1220, 1619
AcoI YGGCCR 5 cut(s) 220, 258, 342, 466, 742
AcsI RAATTY 8 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1560
AfaI GTAC 1 cut(s) 1331
AfiI CCNNNNNNNGG 5 cut(s) 340, 422, 453, 726, 855
AflIII ACRYGT 1 cut(s) 1023
AgeI ACCGGT 1 cut(s) 58
AjnI CCWGG 2 cut(s) 42, 912
AleI CACNNNNGTG 1 cut(s) 380
Alw21I GWGCWC 2 cut(s) 135, 1383
Alw26I GTCTC 4 cut(s) 476, 674, 922, 1597
AlwI GGATC 2 cut(s) 1220, 1619
AoxI GGCC 8 cut(s) 220, 258, 342, 466, 742, 814, 1036, 1324
ApeKI GCWGC 6 cut(s) 5, 389, 1274, 1294, 1431, 1577
ApoI RAATTY 8 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1560
AsiGI ACCGGT 1 cut(s) 58
AspS9I GGNCC 3 cut(s) 910, 1181, 1445
AsuC2I CCSGG 4 cut(s) 142, 341, 451, 1612
AsuHPI GGTGA 5 cut(s) 58, 265, 280, 1300, 1600
AsuII TTCGAA 4 cut(s) 641, 1067, 1119, 1397
AsuNHI GCTAGC 1 cut(s) 958
AvaII GGWCC 3 cut(s) 910, 1181, 1445
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 2 cut(s) 137, 374
BanII GRGCYC 4 cut(s) 135, 147, 1020, 1383
Bbv12I GWGCWC 2 cut(s) 135, 1383
BbvI GCAGC 5 cut(s) 376, 1261, 1306, 1418, 1564
BccI CCATC 8 cut(s) 212, 284, 458, 584, 608, 730, 734, 1550
BceAI ACGGC 3 cut(s) 207, 453, 605
BcgI CGANNNNNNTGC 4 cut(s) 119, 153, 1047, 1081
BciT130I CCWGG 2 cut(s) 44, 914
BciVI GTATCC 1 cut(s) 1161
BcnI CCSGG 4 cut(s) 142, 341, 451, 1612
BcoDI GTCTC 4 cut(s) 476, 674, 922, 1597
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 1 cut(s) 1292
BfuAI ACCTGC 1 cut(s) 1299
BfuI GTATCC 1 cut(s) 1161
BmcAI AGTACT 1 cut(s) 1331
Bme1390I CCNGG 6 cut(s) 44, 142, 341, 451, 914, 1612
Bme18I GGWCC 3 cut(s) 910, 1181, 1445
BmgT120I GGNCC 3 cut(s) 910, 1181, 1445
BmiI GGNNCC 4 cut(s) 139, 376, 911, 1447
BmrFI CCNGG 6 cut(s) 44, 142, 341, 451, 914, 1612
BmrI ACTGGG 1 cut(s) 916
BmsI GCATC 4 cut(s) 760, 1181, 1241, 1514
BmtI GCTAGC 1 cut(s) 962
BmuI ACTGGG 1 cut(s) 916
BpmI CTGGAG 1 cut(s) 1146
Bpu14I TTCGAA 4 cut(s) 641, 1067, 1119, 1397
BpuEI CTTGAG 2 cut(s) 971, 1649
BpuMI CCSGG 4 cut(s) 142, 341, 451, 1612
Bsa29I ATCGAT 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 575
BsaI GGTCTC 2 cut(s) 674, 922
BsaJI CCNNGG 6 cut(s) 255, 531, 849, 942, 993, 1147
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 4 cut(s) 445, 475, 770, 800
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 422, 453, 726, 855
Bse118I RCCGGY 4 cut(s) 58, 125, 377, 1052
Bse1I ACTGG 4 cut(s) 658, 922, 1129, 1327
Bse3DI GCAATG 1 cut(s) 780
Bse8I GATNNNNATC 1 cut(s) 575
BseBI CCWGG 2 cut(s) 44, 914
BseCI ATCGAT 1 cut(s) 887
BseDI CCNNGG 6 cut(s) 255, 531, 849, 942, 993, 1147
BseGI GGATG 9 cut(s) 251, 276, 600, 775, 1084, 1196, 1256, 1529, 1561
BseJI GATNNNNATC 1 cut(s) 575
BseLI CCNNNNNNNGG 5 cut(s) 340, 422, 453, 726, 855
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 1 cut(s) 1368
BseNI ACTGG 4 cut(s) 658, 922, 1129, 1327
BseRI GAGGAG 1 cut(s) 464
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 5 cut(s) 376, 1261, 1306, 1418, 1564
Bsh1236I CGCG 2 cut(s) 851, 1010
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 8 cut(s) 222, 260, 344, 468, 744, 816, 1038, 1326
BshNI GGYRCC 2 cut(s) 137, 374
BshTI ACCGGT 1 cut(s) 58
BshVI ATCGAT 1 cut(s) 887
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 2 cut(s) 135, 1383
BslFI GGGAC 1 cut(s) 1431
BslI CCNNNNNNNGG 5 cut(s) 340, 422, 453, 726, 855
BsmAI GTCTC 4 cut(s) 476, 674, 922, 1597
BsmBI CGTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 1431
BsmI GAATGC 1 cut(s) 1342
BsnI GGCC 8 cut(s) 222, 260, 344, 468, 744, 816, 1038, 1326
Bso31I GGTCTC 2 cut(s) 674, 922
Bsp119I TTCGAA 4 cut(s) 641, 1067, 1119, 1397
Bsp1286I GDGCHC 4 cut(s) 135, 147, 1020, 1383
Bsp143I GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
Bsp19I CCATGG 2 cut(s) 255, 531
Bsp68I TCGCGA 1 cut(s) 1010
BspACI CCGC 9 cut(s) 92, 334, 345, 669, 745, 849, 851, 854, 1434
BspANI GGCC 8 cut(s) 222, 260, 344, 468, 744, 816, 1038, 1326
BspCNI CTCAG 1 cut(s) 1369
BspDI ATCGAT 1 cut(s) 887
BspFNI CGCG 2 cut(s) 851, 1010
BspLI GGNNCC 4 cut(s) 139, 376, 911, 1447
BspMAI CTGCAG 1 cut(s) 1296
BspMI ACCTGC 1 cut(s) 1299
BspOI GCTAGC 1 cut(s) 962
BspPI GGATC 2 cut(s) 1220, 1619
BspT104I TTCGAA 4 cut(s) 641, 1067, 1119, 1397
BspT107I GGYRCC 2 cut(s) 137, 374
BspTNI GGTCTC 2 cut(s) 674, 922
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 4 cut(s) 58, 125, 377, 1052
BsrI ACTGG 4 cut(s) 658, 922, 1129, 1327
BssAI RCCGGY 4 cut(s) 58, 125, 377, 1052
BssECI CCNNGG 6 cut(s) 255, 531, 849, 942, 993, 1147
BssMI GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
BssT1I CCWWGG 4 cut(s) 255, 531, 942, 1147
Bst2UI CCWGG 2 cut(s) 44, 914
Bst4CI ACNGT 3 cut(s) 429, 1466, 1510
Bst6I CTCTTC 1 cut(s) 253
BstBI TTCGAA 4 cut(s) 641, 1067, 1119, 1397
BstC8I GCNNGC 7 cut(s) 131, 383, 387, 673, 818, 960, 1429
BstDEI CTNAG 1 cut(s) 1377
BstDSI CCRYGG 4 cut(s) 255, 531, 849, 993
BstENI CCTNNNNNAGG 1 cut(s) 451
BstF5I GGATG 9 cut(s) 251, 276, 600, 775, 1084, 1196, 1256, 1529, 1561
BstFNI CGCG 2 cut(s) 851, 1010
BstKTI GATC 6 cut(s) 231, 891, 1048, 1228, 1371, 1627
BstMAI GTCTC 4 cut(s) 476, 674, 922, 1597
BstMBI GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 2 cut(s) 44, 914
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 6 cut(s) 42, 140, 339, 449, 912, 1610
BstSFI CTRYAG 1 cut(s) 1292
BstUI CGCG 2 cut(s) 851, 1010
BstV1I GCAGC 5 cut(s) 376, 1261, 1306, 1418, 1564
BstZI CGGCCG 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 887
BsuI GTATCC 1 cut(s) 1161
BsuRI GGCC 8 cut(s) 222, 260, 344, 468, 744, 816, 1038, 1326
BsuTUI ATCGAT 1 cut(s) 887
BtgI CCRYGG 4 cut(s) 255, 531, 849, 993
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 9 cut(s) 251, 276, 600, 775, 1084, 1196, 1256, 1529, 1561
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 161, 651
BtuMI TCGCGA 1 cut(s) 1010
BveI ACCTGC 1 cut(s) 1299
Cac8I GCNNGC 7 cut(s) 131, 383, 387, 673, 818, 960, 1429
Cfr10I RCCGGY 4 cut(s) 58, 125, 377, 1052
Cfr13I GGNCC 3 cut(s) 910, 1181, 1445
Cfr42I CCGCGG 1 cut(s) 852
ClaI ATCGAT 1 cut(s) 887
CseI GACGC 2 cut(s) 1207, 1243
Csp6I GTAC 1 cut(s) 1330
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 4 cut(s) 256, 532, 1024, 1301
CviQI GTAC 1 cut(s) 1330
DdeI CTNAG 1 cut(s) 1377
DpnI GATC 6 cut(s) 230, 890, 1047, 1227, 1370, 1626
DpnII GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
EaeI YGGCCR 5 cut(s) 220, 258, 342, 466, 742
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Ecl136II GAGCTC 2 cut(s) 133, 1381
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 4 cut(s) 255, 531, 942, 1147
Eco24I GRGCYC 4 cut(s) 135, 147, 1020, 1383
Eco31I GGTCTC 2 cut(s) 674, 922
Eco32I GATATC 1 cut(s) 1360
Eco47I GGWCC 3 cut(s) 910, 1181, 1445
Eco52I CGGCCG 1 cut(s) 342
Eco53kI GAGCTC 2 cut(s) 133, 1381
EcoICRI GAGCTC 2 cut(s) 133, 1381
EcoNI CCTNNNNNAGG 1 cut(s) 451
EcoO109I RGGNCCY 2 cut(s) 1181, 1445
EcoRI GAATTC 4 cut(s) 67, 643, 1115, 1560
EcoRII CCWGG 2 cut(s) 42, 912
EcoRV GATATC 1 cut(s) 1360
EcoT14I CCWWGG 4 cut(s) 255, 531, 942, 1147
EcoT38I GRGCYC 4 cut(s) 135, 147, 1020, 1383
ErhI CCWWGG 4 cut(s) 255, 531, 942, 1147
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 4 cut(s) 259, 535, 1027, 1304
FaqI GGGAC 1 cut(s) 1431
FatI CATG 4 cut(s) 255, 531, 1023, 1300
FauI CCCGC 1 cut(s) 847
FokI GGATG 9 cut(s) 238, 263, 587, 782, 1091, 1203, 1263, 1536, 1568
FriOI GRGCYC 4 cut(s) 135, 147, 1020, 1383
FspBI CTAG 1 cut(s) 959
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 8 cut(s) 222, 260, 344, 468, 744, 816, 1038, 1326
HgaI GACGC 2 cut(s) 1207, 1243
Hin1II CATG 4 cut(s) 259, 535, 1027, 1304
HincII GTYRAC 1 cut(s) 861
HindII GTYRAC 1 cut(s) 861
HinfI GANTC 7 cut(s) 110, 362, 638, 806, 933, 1084, 1582
HphI GGTGA 5 cut(s) 58, 265, 280, 1300, 1600
Hpy166II GTNNAC 2 cut(s) 861, 1481
Hpy188III TCNNGA 3 cut(s) 204, 950, 1009
Hpy8I GTNNAC 2 cut(s) 861, 1481
Hpy99I CGWCG 2 cut(s) 199, 758
HpyAV CCTTC 5 cut(s) 49, 201, 324, 1642, 1664
HpyCH4III ACNGT 3 cut(s) 429, 1466, 1510
HpyCH4IV ACGT 1 cut(s) 1493
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 1 cut(s) 1377
HpySE526I ACGT 1 cut(s) 1493
Hsp92II CATG 4 cut(s) 259, 535, 1027, 1304
KspI CCGCGG 1 cut(s) 852
Kzo9I GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
LmnI GCTCC 2 cut(s) 414, 778
Lsp1109I GCAGC 5 cut(s) 376, 1261, 1306, 1418, 1564
LweI GCATC 4 cut(s) 760, 1181, 1241, 1514
MaeI CTAG 1 cut(s) 959
MaeII ACGT 1 cut(s) 1493
MaeIII GTNAC 3 cut(s) 1436, 1466, 1510
MalI GATC 6 cut(s) 230, 890, 1047, 1227, 1370, 1626
MboI GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
MboII GAAGA 5 cut(s) 149, 240, 427, 512, 1010
MhlI GDGCHC 4 cut(s) 135, 147, 1020, 1383
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 9 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1390, 1560
MluNI TGGCCA 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 144, 672
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MseI TTAA 2 cut(s) 99, 1287
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 851
MspR9I CCNGG 6 cut(s) 44, 142, 341, 451, 914, 1612
Mva1269I GAATGC 1 cut(s) 1342
MvaI CCWGG 2 cut(s) 44, 914
MvnI CGCG 2 cut(s) 851, 1010
MwoI GCNNNNNNNGC 1 cut(s) 503
NciI CCSGG 4 cut(s) 142, 341, 451, 1612
NcoI CCATGG 2 cut(s) 255, 531
NdeII GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
NheI GCTAGC 1 cut(s) 958
NlaIII CATG 4 cut(s) 259, 535, 1027, 1304
NlaIV GGNNCC 4 cut(s) 139, 376, 911, 1447
NmeAIII GCCGAG 1 cut(s) 846
NmuCI GTSAC 2 cut(s) 1466, 1510
NruI TCGCGA 1 cut(s) 1010
NspI RCATGY 1 cut(s) 1027
NspV TTCGAA 4 cut(s) 641, 1067, 1119, 1397
OliI CACNNNNGTG 1 cut(s) 380
PciI ACATGT 1 cut(s) 1023
PctI GAATGC 1 cut(s) 1342
PfeI GAWTC 7 cut(s) 110, 362, 638, 806, 933, 1084, 1582
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 449
PinAI ACCGGT 1 cut(s) 58
PpuMI RGGWCCY 2 cut(s) 1181, 1445
PscI ACATGT 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 135, 1383
Psp5II RGGWCCY 2 cut(s) 1181, 1445
Psp6I CCWGG 2 cut(s) 42, 912
PspGI CCWGG 2 cut(s) 42, 912
PspN4I GGNNCC 4 cut(s) 139, 376, 911, 1447
PspPI GGNCC 3 cut(s) 910, 1181, 1445
PspPPI RGGWCCY 2 cut(s) 1181, 1445
PstI CTGCAG 1 cut(s) 1296
RruI TCGCGA 1 cut(s) 1010
RsaI GTAC 1 cut(s) 1331
RsaNI GTAC 1 cut(s) 1330
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 2 cut(s) 135, 1383
SacII CCGCGG 1 cut(s) 852
SaqAI TTAA 2 cut(s) 99, 1287
Sau3AI GATC 6 cut(s) 228, 888, 1045, 1225, 1368, 1624
Sau96I GGNCC 3 cut(s) 910, 1181, 1445
ScaI AGTACT 1 cut(s) 1331
ScrFI CCNGG 6 cut(s) 44, 142, 341, 451, 914, 1612
SduI GDGCHC 4 cut(s) 135, 147, 1020, 1383
SfaNI GCATC 4 cut(s) 760, 1181, 1241, 1514
SfcI CTRYAG 1 cut(s) 1292
Sfr303I CCGCGG 1 cut(s) 852
SfuI TTCGAA 4 cut(s) 641, 1067, 1119, 1397
SgrAI CRCCGGYG 3 cut(s) 125, 377, 1052
SgrBI CCGCGG 1 cut(s) 852
SinI GGWCC 3 cut(s) 910, 1181, 1445
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 950, 1664
SmoI CTYRAG 2 cut(s) 950, 1664
Sse9I AATT 9 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1390, 1560
SsiI CCGC 9 cut(s) 92, 334, 345, 669, 745, 849, 851, 854, 1434
SspMI CTAG 1 cut(s) 959
SstI GAGCTC 2 cut(s) 135, 1383
StyD4I CCNGG 6 cut(s) 42, 140, 339, 449, 912, 1610
StyI CCWWGG 4 cut(s) 255, 531, 942, 1147
TaaI ACNGT 3 cut(s) 429, 1466, 1510
TaiI ACGT 1 cut(s) 1496
TasI AATT 9 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1390, 1560
TatI WGTACW 1 cut(s) 1329
TauI GCSGC 4 cut(s) 347, 747, 851, 854
TfiI GAWTC 7 cut(s) 110, 362, 638, 806, 933, 1084, 1582
Tru1I TTAA 2 cut(s) 99, 1287
Tru9I TTAA 2 cut(s) 99, 1287
TscAI CASTG 2 cut(s) 168, 658
TseFI GTSAC 2 cut(s) 1466, 1510
TseI GCWGC 6 cut(s) 5, 389, 1274, 1294, 1431, 1577
Tsp45I GTSAC 2 cut(s) 1466, 1510
TspDTI ATGAA 3 cut(s) 240, 1289, 1553
TspGWI ACGGA 5 cut(s) 183, 341, 774, 1010, 1455
TspRI CASTG 2 cut(s) 168, 658
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 3 cut(s) 910, 1181, 1445
XagI CCTNNNNNAGG 1 cut(s) 451
XapI RAATTY 8 cut(s) 67, 115, 267, 282, 558, 643, 1115, 1560
XceI RCATGY 1 cut(s) 1027
XspI CTAG 1 cut(s) 959
ZrmI AGTACT 1 cut(s) 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.