Rroxscaffold_6G00430880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002437
Physical Location & Seq
Reverse (-)
50957175 .. 50959557
2383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430880.1

Sequence Viewer

Length: 1164 bp
ATGGCTGCCAAGAATCTCAATGCTTTCCGTATTCTCACCTTACTCTGCTTCGTTCTTTTACGTTTTGCAAACTCACTGAACTATCCCGCCGTTTTCAACTTTGGTGATTCGAACTCCGACACCGGCGAGCTGACCGCCGGAATCGGTTTCCGGCTGCCTCATCCTTATGGAGAGCTTTACTTCGAGTCTTCGTTGTCATCGGGTGGGAGGTTTTCCGATGGCCGTCTCATCATAGATTTTCTCATGGGTGCAATGAACCTTCCATTTCTAAATCCCTATTCGGATTCAGTTGGCATGCCAAGTTTCCGAAATGGGTGCAATTTTGCTGCTGGAGGGGCTACTACACTTCCAGCCGCTGCAACTTCTACCTGCCCCTTTTCATTTGGGATTCAAGTATCTCAGTTTTCTCGATTCAAAGCTCGGGTTCTTCAATTGCTTGCTGCTAAAGGTAAGGAACTCAAGAATTATCTACCCTCGGAAGATTCCTTTCAGAAGGGGCTTTACGTGTTTGATATAGGCCAAAATGATCTTGCTGGTGCATTTTATTCTAAAACACTGGATCAGATTCTTGCTTTTATTCCAACAATTTTGGCAGAGTTTGAATCAGGAATAAAGAGATTATATGATCAAGGTGCTAGGAACTTTTGGATACACAACACAGGTCCTCTGGGGTGCTTGACTCAGAATGTGGCCACATTTGGAACCAACCCATCAAAGGTTGATGAGCATGGATGTGTTAGCGCACACAACCAAGCAGCTAAAATCTTCAACCTGCAGCTTCATGCTCTCAGTAAAAAGTTGCAAGGCCAATACTCTGATGCACATGTCTCATATGTTGATATCTTCTCTATAAAATATGACCTTATTGCAAATTATTCTCGATACGGGTTTCAGCAACCTATTATGGCTTGCTGTGGGTATGGAGGTCCACCTTTGAACTATGACAGTCGGGTTTCTTGTGGATCAACAAGGGTCTTGAATGGCACGTCTGTCACAGCCAAAGGGTGTAGCAATGGTACTGTGCACGTTAATTGGGATGGAATTCATTACACAGAAGCTGCAAATCAGTTTGTCTCATCAAAGATACTCACCGGAAAGTATTCCGATCCTCCTTTGTCGAACAACATTTCTTTCCTCTCAAGTTCTAAAACCCCAATGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.19

Weight (kDa)

8.48

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 361 7.7e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 377, 780
AciI CCGC 3 cut(s) 87, 135, 354
AclWI GGATC 3 cut(s) 567, 970, 1100
AcoI YGGCCR 2 cut(s) 220, 690
AcsI RAATTY 1 cut(s) 1041
AfaI GTAC 1 cut(s) 1018
AfiI CCNNNNNNNGG 2 cut(s) 715, 1158
AflIII ACRYGT 2 cut(s) 504, 823
AgsI TTSAA 8 cut(s) 97, 392, 415, 431, 602, 769, 937, 979
AjiI CACGTC 1 cut(s) 987
AluBI AGCT 6 cut(s) 130, 175, 419, 758, 778, 1058
AluI AGCT 6 cut(s) 130, 175, 419, 758, 778, 1058
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 3 cut(s) 230, 832, 1078
Alw44I GTGCAC 1 cut(s) 1022
AlwI GGATC 3 cut(s) 567, 970, 1100
AlwNI CAGNNNCTG 2 cut(s) 356, 1058
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 4 cut(s) 220, 517, 690, 805
ApaLI GTGCAC 1 cut(s) 1022
ApeKI GCWGC 8 cut(s) 5, 154, 326, 356, 440, 755, 775, 1058
ApoI RAATTY 1 cut(s) 1041
Asp700I GAANNNNTTC 1 cut(s) 1099
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 2 cut(s) 662, 926
AsuHPI GGTGA 3 cut(s) 28, 116, 1081
AsuII TTCGAA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 420
AvaII GGWCC 2 cut(s) 662, 926
BaeGI GKGCMC 1 cut(s) 1026
BalI TGGCCA 1 cut(s) 692
BarI GAAGNNNNNNTAC 2 cut(s) 485, 517
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 7 cut(s) 141, 313, 343, 427, 767, 787, 1045
BccI CCATC 3 cut(s) 212, 718, 1031
BceAI ACGGC 2 cut(s) 74, 207
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BciVI GTATCC 1 cut(s) 642
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 3 cut(s) 230, 832, 1078
BfaI CTAG 1 cut(s) 636
BfmI CTRYAG 1 cut(s) 773
BfuAI ACCTGC 2 cut(s) 377, 780
BfuI GTATCC 1 cut(s) 642
BisI GCNGC 9 cut(s) 6, 155, 327, 354, 357, 441, 756, 776, 1059
BlsI GCNGC 9 cut(s) 7, 156, 328, 355, 358, 442, 757, 777, 1060
Bme18I GGWCC 2 cut(s) 662, 926
BmeT110I CYCGRG 1 cut(s) 420
BmgBI CACGTC 1 cut(s) 987
BmgT120I GGNCC 2 cut(s) 662, 926
BmiI GGNNCC 1 cut(s) 703
BmsI GCATC 1 cut(s) 808
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 110
BpuEI CTTGAG 2 cut(s) 443, 1123
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 1091
Bsc4I CCNNNNNNNGG 2 cut(s) 715, 1158
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 258, 1018
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 3 cut(s) 160, 737, 1042
BseLI CCNNNNNNNGG 2 cut(s) 715, 1158
BseMI GCAATG 2 cut(s) 258, 1018
BseMII CTCAG 3 cut(s) 413, 695, 802
BseNI ACTGG 1 cut(s) 561
BseSI GKGCMC 1 cut(s) 1026
BseXI GCAGC 7 cut(s) 141, 313, 343, 427, 767, 787, 1045
BshFI GGCC 4 cut(s) 222, 519, 692, 807
BsiHKAI GWGCWC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 4 cut(s) 123, 138, 151, 1092
BslI CCNNNNNNNGG 2 cut(s) 715, 1158
BsmAI GTCTC 3 cut(s) 230, 832, 1078
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 4 cut(s) 222, 519, 692, 807
BsoBI CYCGRG 1 cut(s) 420
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp143I GATC 5 cut(s) 526, 559, 625, 962, 1105
BspACI CCGC 3 cut(s) 87, 135, 354
BspANI GGCC 4 cut(s) 222, 519, 692, 807
BspCNI CTCAG 3 cut(s) 412, 694, 801
BspLI GGNNCC 1 cut(s) 703
BspMAI CTGCAG 1 cut(s) 777
BspMI ACCTGC 2 cut(s) 377, 780
BspPI GGATC 3 cut(s) 567, 970, 1100
BspT104I TTCGAA 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 258, 1018
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 561
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 5 cut(s) 526, 559, 625, 962, 1105
Bst4CI ACNGT 2 cut(s) 947, 1021
BstBAI YACGTR 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 4 cut(s) 128, 296, 438, 910
BstDEI CTNAG 3 cut(s) 399, 681, 788
BstF5I GGATG 3 cut(s) 160, 737, 1042
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 5 cut(s) 529, 562, 628, 965, 1108
BstMAI GTCTC 3 cut(s) 230, 832, 1078
BstMBI GATC 5 cut(s) 526, 559, 625, 962, 1105
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNSI RCATGY 2 cut(s) 298, 827
BstSFI CTRYAG 1 cut(s) 773
BstSLI GKGCMC 1 cut(s) 1026
BstV1I GCAGC 7 cut(s) 141, 313, 343, 427, 767, 787, 1045
BstV2I GAAGAC 1 cut(s) 180
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 4 cut(s) 222, 519, 692, 807
BtrI CACGTC 1 cut(s) 987
BtsCI GGATG 3 cut(s) 160, 737, 1042
BtsIMutI CAGTG 2 cut(s) 74, 554
BveI ACCTGC 2 cut(s) 377, 780
Cac8I GCNNGC 4 cut(s) 128, 296, 438, 910
CaiI CAGNNNCTG 2 cut(s) 356, 1058
CfoI GCGC 1 cut(s) 743
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 662, 926
Csp6I GTAC 1 cut(s) 1017
CviAII CATG 5 cut(s) 244, 295, 728, 782, 824
CviQI GTAC 1 cut(s) 1017
DdeI CTNAG 3 cut(s) 399, 681, 788
DpnI GATC 5 cut(s) 528, 561, 627, 964, 1107
DpnII GATC 5 cut(s) 526, 559, 625, 962, 1105
EaeI YGGCCR 2 cut(s) 220, 690
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 2 cut(s) 662, 926
Eco88I CYCGRG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 662
EcoRI GAATTC 1 cut(s) 1041
EcoRV GATATC 1 cut(s) 841
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 5 cut(s) 247, 298, 731, 785, 827
FatI CATG 5 cut(s) 243, 294, 727, 781, 823
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 832
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 9 cut(s) 6, 155, 327, 354, 357, 441, 756, 776, 1059
FokI GGATG 3 cut(s) 147, 744, 1049
Fsp4HI GCNGC 9 cut(s) 6, 155, 327, 354, 357, 441, 756, 776, 1059
FspBI CTAG 1 cut(s) 636
GlaI GCGC 1 cut(s) 742
GluI GCNGC 9 cut(s) 6, 155, 327, 354, 357, 441, 756, 776, 1059
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 4 cut(s) 222, 519, 692, 807
HapII CCGG 4 cut(s) 123, 138, 151, 1092
HhaI GCGC 1 cut(s) 743
Hin1II CATG 5 cut(s) 247, 298, 731, 785, 827
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HpaII CCGG 4 cut(s) 123, 138, 151, 1092
HphI GGTGA 3 cut(s) 28, 116, 1081
Hpy166II GTNNAC 2 cut(s) 929, 1024
Hpy188III TCNNGA 5 cut(s) 408, 460, 606, 879, 976
Hpy8I GTNNAC 2 cut(s) 929, 1024
HpyAV CCTTC 2 cut(s) 269, 487
HpyCH4III ACNGT 2 cut(s) 947, 1021
HpyCH4IV ACGT 4 cut(s) 61, 504, 986, 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 3 cut(s) 399, 681, 788
HpySE526I ACGT 4 cut(s) 61, 504, 986, 1026
Hsp92II CATG 5 cut(s) 247, 298, 731, 785, 827
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 5 cut(s) 526, 559, 625, 962, 1105
Lsp1109I GCAGC 7 cut(s) 141, 313, 343, 427, 767, 787, 1045
LweI GCATC 1 cut(s) 808
MaeI CTAG 1 cut(s) 636
MaeII ACGT 4 cut(s) 61, 504, 986, 1026
MaeIII GTNAC 1 cut(s) 991
MalI GATC 5 cut(s) 528, 561, 627, 964, 1107
MboI GATC 5 cut(s) 526, 559, 625, 962, 1105
MboII GAAGA 5 cut(s) 180, 419, 491, 757, 835
MfeI CAATTG 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 1026
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 7 cut(s) 319, 431, 463, 585, 871, 1030, 1041
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 2 cut(s) 194, 673
MmeI TCCRAC 2 cut(s) 141, 605
MnlI CCTC 8 cut(s) 168, 201, 326, 484, 675, 917, 1119, 1145
Mox20I TGGCCA 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 1099
MscI TGGCCA 1 cut(s) 692
MseI TTAA 1 cut(s) 1029
MslI CAYNNNNRTG 2 cut(s) 165, 732
Msp20I TGGCCA 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 123, 138, 151, 1092
MunI CAATTG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeI CATATG 1 cut(s) 832
NdeII GATC 5 cut(s) 526, 559, 625, 962, 1105
NlaIII CATG 5 cut(s) 247, 298, 731, 785, 827
NlaIV GGNNCC 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 991
NspI RCATGY 2 cut(s) 298, 827
NspV TTCGAA 1 cut(s) 110
PaeI GCATGC 1 cut(s) 298
PciI ACATGT 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 9 cut(s) 13, 107, 141, 284, 388, 411, 482, 565, 602
PkrI GCNGC 9 cut(s) 7, 156, 328, 355, 358, 442, 757, 777, 1060
PleI GAGTC 2 cut(s) 193, 673
PpsI GAGTC 2 cut(s) 193, 673
Ppu21I YACGTR 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 662
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 2 cut(s) 662, 926
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 1 cut(s) 777
PstNI CAGNNNCTG 2 cut(s) 356, 1058
RsaI GTAC 1 cut(s) 1018
RsaNI GTAC 1 cut(s) 1017
RseI CAYNNNNRTG 2 cut(s) 165, 732
SaqAI TTAA 1 cut(s) 1029
SatI GCNGC 9 cut(s) 6, 155, 327, 354, 357, 441, 756, 776, 1059
Sau3AI GATC 5 cut(s) 526, 559, 625, 962, 1105
Sau96I GGNCC 2 cut(s) 662, 926
SchI GAGTC 2 cut(s) 194, 673
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 1 cut(s) 808
SfcI CTRYAG 1 cut(s) 773
SfuI TTCGAA 1 cut(s) 110
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 662, 926
SmiMI CAYNNNNRTG 2 cut(s) 165, 732
SmlI CTYRAG 2 cut(s) 458, 1138
SmoI CTYRAG 2 cut(s) 458, 1138
SphI GCATGC 1 cut(s) 298
Sse9I AATT 7 cut(s) 319, 431, 463, 585, 871, 1030, 1041
SsiI CCGC 3 cut(s) 87, 135, 354
SspMI CTAG 1 cut(s) 636
TaaI ACNGT 2 cut(s) 947, 1021
TaiI ACGT 4 cut(s) 64, 507, 989, 1029
TaqI TCGA 5 cut(s) 110, 183, 409, 880, 1118
TasI AATT 7 cut(s) 319, 431, 463, 585, 871, 1030, 1041
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 9 cut(s) 13, 107, 141, 284, 388, 411, 482, 565, 602
Tru1I TTAA 1 cut(s) 1029
Tru9I TTAA 1 cut(s) 1029
TscAI CASTG 2 cut(s) 81, 561
TseFI GTSAC 1 cut(s) 991
TseI GCWGC 8 cut(s) 5, 154, 326, 356, 440, 755, 775, 1058
Tsp45I GTSAC 1 cut(s) 991
TspDTI ATGAA 4 cut(s) 269, 369, 770, 1034
TspGWI ACGGA 1 cut(s) 17
TspRI CASTG 2 cut(s) 81, 561
VneI GTGCAC 1 cut(s) 1022
VpaK11BI GGWCC 2 cut(s) 662, 926
XapI RAATTY 1 cut(s) 1041
XceI RCATGY 2 cut(s) 298, 827
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.