Rmu_sc0026189.1_g000004

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0026189.1
OG0002437
Physical Location & Seq
Forward (+)
17939 .. 18951
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0026189.1_g000004.1.cds

Sequence Viewer

Length: 474 bp
atggctgcgaagaatctcaatgcttttcgtattctcatcttcctctgcttcgttcttttacgttttgcaaactcactgaactatcccgccgttttcaactttggtgattcgaactccgacaccggcgagctgaccgccggcgtaggtttccggcttcctcatccttatggaaagctttacttcaatgcttcgttgtcatcgggtgggaggttttccgatggccgtctcatcatagattttctcatgggtgcaatgaaccttccatttctaaatccctatttggattcagttggaatgccaagtttccgaaatgggtgcaattttgctgctggaggggctactacacatccagcaactgcatcatctgtctgccctttttcatttggggttcaggtctctcagttttcgcgattcaaatccagggctcttcaattgctagctgctaaaggtatatatagtcatgaactttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

157

Amino Acids

16.98

Weight (kDa)

9.41

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 409
AciI CCGC 2 cut(s) 87, 135
AcoI YGGCCR 1 cut(s) 220
AgsI TTSAA 4 cut(s) 97, 184, 415, 431
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 3 cut(s) 130, 175, 440
AluI AGCT 3 cut(s) 130, 175, 440
Alw26I GTCTC 2 cut(s) 230, 400
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 5, 326, 440
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 110
AsuNHI GCTAGC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 313, 427
BccI CCATC 1 cut(s) 212
BceAI ACGGC 2 cut(s) 74, 207
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BciT130I CCWGG 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 230, 400
BfaI CTAG 1 cut(s) 437
BisI GCNGC 3 cut(s) 6, 327, 441
BlsI GCNGC 3 cut(s) 7, 328, 442
Bme1390I CCNGG 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 421
BmsI GCATC 1 cut(s) 368
BmtI GCTAGC 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 415
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 420
Bse118I RCCGGY 2 cut(s) 122, 137
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 415
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 2 cut(s) 160, 346
BseJI GATNNNNATC 1 cut(s) 415
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 313, 427
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 1 cut(s) 222
BsiSI CCGG 3 cut(s) 123, 138, 151
BsmAI GTCTC 2 cut(s) 230, 400
BsmBI CGTCTC 1 cut(s) 230
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 400
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 427
Bsp68I TCGCGA 1 cut(s) 409
BspACI CCGC 2 cut(s) 87, 135
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 460
BspOI GCTAGC 1 cut(s) 440
BspQI GCTCTTC 1 cut(s) 432
BspT104I TTCGAA 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 2 cut(s) 122, 137
BssAI RCCGGY 2 cut(s) 122, 137
BssECI CCNNGG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 3 cut(s) 128, 139, 438
BstDEI CTNAG 1 cut(s) 399
BstF5I GGATG 2 cut(s) 160, 346
BstFNI CGCG 1 cut(s) 409
BstMAI GTCTC 2 cut(s) 230, 400
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 419
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 2 cut(s) 313, 427
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 2 cut(s) 160, 346
BtsIMutI CAGTG 1 cut(s) 74
BtuMI TCGCGA 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 128, 139, 438
CaiI CAGNNNCTG 1 cut(s) 356
CciI TCATGA 1 cut(s) 460
Cfr10I RCCGGY 2 cut(s) 122, 137
CviAII CATG 2 cut(s) 244, 461
CviJI RGCY 8 cut(s) 5, 130, 154, 175, 222, 338, 425, 440
CviKI_1 RGCY 8 cut(s) 5, 130, 154, 175, 222, 338, 425, 440
DdeI CTNAG 1 cut(s) 399
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 427
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 2 cut(s) 247, 464
FaiI YATR 7 cut(s) 168, 233, 245, 452, 454, 456, 462
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FatI CATG 2 cut(s) 243, 460
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 6, 327, 441
FokI GGATG 2 cut(s) 147, 333
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 3 cut(s) 6, 327, 441
FspBI CTAG 1 cut(s) 437
GluI GCNGC 3 cut(s) 6, 327, 441
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 1 cut(s) 222
HapII CCGG 3 cut(s) 123, 138, 151
Hin1II CATG 2 cut(s) 247, 464
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 13, 107, 284, 411
HpaII CCGG 3 cut(s) 123, 138, 151
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 118, 217, 308
Hpy188III TCNNGA 3 cut(s) 408, 461, 471
HpyAV CCTTC 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 4 cut(s) 68, 251, 318, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 2 cut(s) 247, 464
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
LguI GCTCTTC 1 cut(s) 432
LpnPI CCDG 8 cut(s) 136, 151, 164, 315, 363, 377, 406, 433
Lsp1109I GCAGC 2 cut(s) 313, 427
LweI GCATC 1 cut(s) 368
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 61
MboII GAAGA 3 cut(s) 22, 31, 419
MfeI CAATTG 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 2 cut(s) 319, 431
MmeI TCCRAC 2 cut(s) 141, 271
MnlI CCTC 4 cut(s) 53, 168, 201, 326
MreI CGCCGGCG 1 cut(s) 137
MroNI GCCGGC 1 cut(s) 137
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 3 cut(s) 123, 138, 151
MspR9I CCNGG 1 cut(s) 421
MunI CAATTG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 1 cut(s) 421
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 335
NaeI GCCGGC 1 cut(s) 139
NgoMIV GCCGGC 1 cut(s) 137
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 2 cut(s) 247, 464
NruI TCGCGA 1 cut(s) 409
NspV TTCGAA 1 cut(s) 110
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 432
PctI GAATGC 1 cut(s) 300
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 4 cut(s) 13, 107, 284, 411
PkrI GCNGC 3 cut(s) 7, 328, 442
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PstNI CAGNNNCTG 1 cut(s) 356
RruI TCGCGA 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 432
SatI GCNGC 3 cut(s) 6, 327, 441
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 427
SetI ASST 9 cut(s) 64, 132, 148, 177, 212, 261, 396, 442, 451
SfaNI GCATC 1 cut(s) 368
SfuI TTCGAA 1 cut(s) 110
SgrAI CRCCGGYG 2 cut(s) 122, 137
SmiMI CAYNNNNRTG 1 cut(s) 165
Sse9I AATT 2 cut(s) 319, 431
SsiI CCGC 2 cut(s) 87, 135
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 419
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 110
TasI AATT 2 cut(s) 319, 431
TfiI GAWTC 4 cut(s) 13, 107, 284, 411
TscAI CASTG 1 cut(s) 81
TseI GCWGC 3 cut(s) 5, 326, 440
TspDTI ATGAA 2 cut(s) 269, 369
TspRI CASTG 1 cut(s) 81
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.