MD04G1209300.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002437
Physical Location & Seq
Forward (+)
29446590 .. 29448366
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1209300.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGCTGCCAAAGCCTGCGTCATTCAAATTCTCACATTGATTTCCATCTCCTTACCTCTTTCCAAATCCATCCACTTCAACCACCCTGCCGTTTTCAACTTTGGCGATTCGAATTCCGACACTGGCAATCTTGTTTCCGCCGGCATTGAGAGCATTCTTCCTCCTTATGGACAATCTTATTTCAAAACCCCATCTGGGAGATACTCCGATGGCCGCCTCATCGTTGATTTTCTTTTGAATGCAATGCAGCTTCCATTTCTCAATGCCTATCTGGATTCCGTTGGCCTGCCAATATTCAAAAAGGGTTGCAACTTTGCCGCGGCAGGGTCAACTATACTTCCAGCAACTGCAACATCCATCAGCCCGTTTTCTTTCGGGGTCCAGGTCTCCCAGTTTTTTAGATTCAAAGCCAAGGTTCTTGAATTGCTAGCTAGAGGTAGGAGCTTTCCCAAGTATCTGCCCGCGGAAGTTTTTTTCGCGAAGGGGCTCTACATGTTCGATATAGGCCAGAATGATCTCGCTGGTGCATTTTATTCGAAATCATTGGATCAGATTCTCGCTTCCATTCCAATGATTTTGACAGAATTCGAAACAGGAATAAAGAGATTATATGACCAAGGAGGTAGGAACTTTTGGATACACAACACGGGTCCTCTCGGGTGCTTGACGCAGAATGTTGCCAAGTTTGGAACTGATCCATCGAAGCTTGACGAACAAGGATGCGTCAGCGACCACAACCAAGCTGCTAAACTCTTTAACCTGCAGCTTCATGCCCTCACCAAAAAGTTGCAAGGCCAGTACTCGGATGCAAGTGTCATTTATGTTGATATCTTTTCGATCAAATCTGAGCTCATATCAAATTATTCGAAAAATGGATTTGAACAACCTATTATGGCTTGCTGCGGGTATGGAGGTCCACCGTTGAACTATGACAGTCAGATTACCTGTGGACAAACGAAGACCTTGAACGGAACAATTGTCACAGCAAAAGGATGCACCGATAGTTCTGATTACATTAGTTGGGATGGAATTCATTACACCGAAGCTGCGAACCAGTTTGTTTCGTCAAAGATACTCACTGGAAAGTATTCTGATCCGCCCTTCGCGGACAAAATGCCTTTCCTTTTGAAGCTCAAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.56

Weight (kDa)

8.29

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 357 1.6e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 768
AccII CGCG 4 cut(s) 320, 464, 479, 1106
AciI CCGC 9 cut(s) 138, 214, 318, 320, 462, 464, 903, 1097, 1106
AclWI GGATC 3 cut(s) 555, 689, 1088
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 4 cut(s) 27, 112, 584, 1029
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 4 cut(s) 167, 195, 324, 802
AflIII ACRYGT 1 cut(s) 492
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 9 cut(s) 250, 431, 444, 706, 743, 766, 850, 1046, 1132
AluI AGCT 9 cut(s) 250, 431, 444, 706, 743, 766, 850, 1046, 1132
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 3 cut(s) 555, 689, 1088
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 4 cut(s) 211, 283, 505, 793
ApeKI GCWGC 6 cut(s) 5, 247, 743, 763, 900, 1046
ApoI RAATTY 4 cut(s) 27, 112, 584, 1029
ArsI GACNNNNNNTTYG 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 1087
AspS9I GGNCC 3 cut(s) 379, 650, 914
AsuHPI GGTGA 1 cut(s) 769
AsuII TTCGAA 4 cut(s) 110, 536, 588, 866
AsuNHI GCTAGC 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 3 cut(s) 379, 650, 914
BanII GRGCYC 2 cut(s) 489, 852
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 5 cut(s) 259, 730, 775, 887, 1033
BccI CCATC 7 cut(s) 53, 77, 199, 203, 365, 706, 1019
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BciT130I CCWGG 1 cut(s) 383
BciVI GTATCC 1 cut(s) 630
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 2 cut(s) 428, 432
BfmI CTRYAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 768
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 9 cut(s) 6, 214, 248, 318, 321, 744, 764, 901, 1047
BlsI GCNGC 9 cut(s) 7, 215, 249, 319, 322, 745, 765, 902, 1048
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 1 cut(s) 383
Bme18I GGWCC 3 cut(s) 379, 650, 914
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 379, 650, 914
BmiI GGNNCC 2 cut(s) 380, 651
BmrFI CCNGG 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 3 cut(s) 710, 796, 983
BmtI GCTAGC 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 965
Bpu14I TTCGAA 4 cut(s) 110, 536, 588, 866
BpuEI CTTGAG 1 cut(s) 1118
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 4 cut(s) 318, 411, 462, 616
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 195, 324, 802
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 5 cut(s) 127, 391, 796, 1054, 1084
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 4 cut(s) 318, 411, 462, 616
BseGI GGATG 6 cut(s) 69, 353, 725, 811, 998, 1030
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 4 cut(s) 167, 195, 324, 802
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 5 cut(s) 127, 391, 796, 1054, 1084
BseXI GCAGC 5 cut(s) 259, 730, 775, 887, 1033
Bsh1236I CGCG 4 cut(s) 320, 464, 479, 1106
BshFI GGCC 4 cut(s) 213, 285, 507, 795
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 4 cut(s) 167, 195, 324, 802
BsmAI GTCTC 1 cut(s) 391
BsmI GAATGC 2 cut(s) 153, 244
BsnI GGCC 4 cut(s) 213, 285, 507, 795
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 1 cut(s) 656
Bsp119I TTCGAA 4 cut(s) 110, 536, 588, 866
Bsp1286I GDGCHC 2 cut(s) 489, 852
Bsp143I GATC 5 cut(s) 514, 547, 694, 837, 1093
Bsp68I TCGCGA 1 cut(s) 479
BspACI CCGC 9 cut(s) 138, 214, 318, 320, 462, 464, 903, 1097, 1106
BspANI GGCC 4 cut(s) 213, 285, 507, 795
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 4 cut(s) 320, 464, 479, 1106
BspLI GGNNCC 2 cut(s) 380, 651
BspMAI CTGCAG 1 cut(s) 765
BspMI ACCTGC 1 cut(s) 768
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 3 cut(s) 555, 689, 1088
BspT104I TTCGAA 4 cut(s) 110, 536, 588, 866
BspTNI GGTCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 5 cut(s) 127, 391, 796, 1054, 1084
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 4 cut(s) 318, 411, 462, 616
BssMI GATC 5 cut(s) 514, 547, 694, 837, 1093
BssT1I CCWWGG 2 cut(s) 411, 616
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 2 cut(s) 921, 935
BstBI TTCGAA 4 cut(s) 110, 536, 588, 866
BstC8I GCNNGC 6 cut(s) 16, 142, 287, 429, 462, 898
BstDEI CTNAG 1 cut(s) 846
BstDSI CCRYGG 2 cut(s) 318, 462
BstF5I GGATG 6 cut(s) 69, 353, 725, 811, 998, 1030
BstFNI CGCG 4 cut(s) 320, 464, 479, 1106
BstKTI GATC 5 cut(s) 517, 550, 697, 840, 1096
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 5 cut(s) 514, 547, 694, 837, 1093
BstMWI GCNNNNNNNGC 2 cut(s) 11, 150
BstNI CCWGG 1 cut(s) 383
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 1 cut(s) 381
BstSFI CTRYAG 1 cut(s) 761
BstUI CGCG 4 cut(s) 320, 464, 479, 1106
BstV1I GCAGC 5 cut(s) 259, 730, 775, 887, 1033
BstV2I GAAGAC 1 cut(s) 965
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 4 cut(s) 213, 285, 507, 795
BtgI CCRYGG 2 cut(s) 318, 462
BtsCI GGATG 6 cut(s) 69, 353, 725, 811, 998, 1030
BtsIMutI CAGTG 2 cut(s) 120, 1077
BtuMI TCGCGA 1 cut(s) 479
BveI ACCTGC 1 cut(s) 768
Cac8I GCNNGC 6 cut(s) 16, 142, 287, 429, 462, 898
CaiI CAGNNNCTG 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 379, 650, 914
Cfr42I CCGCGG 2 cut(s) 321, 465
CseI GACGC 3 cut(s) 7, 676, 712
Csp6I GTAC 1 cut(s) 799
CviAII CATG 2 cut(s) 493, 770
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 1 cut(s) 846
DpnI GATC 5 cut(s) 516, 549, 696, 839, 1095
DpnII GATC 5 cut(s) 514, 547, 694, 837, 1093
EaeI YGGCCR 1 cut(s) 211
EciI GGCGGA 2 cut(s) 127, 1086
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 2 cut(s) 411, 616
Eco24I GRGCYC 2 cut(s) 489, 852
Eco31I GGTCTC 1 cut(s) 391
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 3 cut(s) 379, 650, 914
Eco53kI GAGCTC 1 cut(s) 850
Eco88I CYCGRG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 3 cut(s) 112, 584, 1029
EcoRII CCWGG 1 cut(s) 381
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 411, 616
EcoT38I GRGCYC 2 cut(s) 489, 852
ErhI CCWWGG 2 cut(s) 411, 616
FaeI CATG 2 cut(s) 496, 773
FatI CATG 2 cut(s) 492, 769
FauI CCCGC 2 cut(s) 469, 896
Fnu4HI GCNGC 9 cut(s) 6, 214, 248, 318, 321, 744, 764, 901, 1047
FokI GGATG 6 cut(s) 56, 340, 732, 818, 1005, 1037
FriOI GRGCYC 2 cut(s) 489, 852
Fsp4HI GCNGC 9 cut(s) 6, 214, 248, 318, 321, 744, 764, 901, 1047
FspBI CTAG 2 cut(s) 428, 432
GluI GCNGC 9 cut(s) 6, 214, 248, 318, 321, 744, 764, 901, 1047
HaeIII GGCC 4 cut(s) 213, 285, 507, 795
HapII CCGG 1 cut(s) 141
HgaI GACGC 3 cut(s) 7, 676, 712
Hin1II CATG 2 cut(s) 496, 773
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 4 cut(s) 107, 275, 402, 553
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 769
Hpy166II GTNNAC 3 cut(s) 330, 917, 950
Hpy188I TCNGA 9 cut(s) 118, 208, 552, 805, 847, 939, 1009, 1093, 1142
Hpy188III TCNNGA 3 cut(s) 272, 419, 478
Hpy8I GTNNAC 3 cut(s) 330, 917, 950
HpyAV CCTTC 2 cut(s) 475, 1111
HpyCH4III ACNGT 2 cut(s) 921, 935
HpyCH4V TGCA 9 cut(s) 242, 247, 309, 350, 527, 763, 790, 809, 996
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 150
HpyF3I CTNAG 1 cut(s) 846
Hsp92II CATG 2 cut(s) 496, 773
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
KspI CCGCGG 2 cut(s) 321, 465
Kzo9I GATC 5 cut(s) 514, 547, 694, 837, 1093
LmnI GCTCC 1 cut(s) 441
Lsp1109I GCAGC 5 cut(s) 259, 730, 775, 887, 1033
LweI GCATC 3 cut(s) 710, 796, 983
MaeI CTAG 2 cut(s) 428, 432
MaeIII GTNAC 1 cut(s) 979
MalI GATC 5 cut(s) 516, 549, 696, 839, 1095
MboI GATC 5 cut(s) 514, 547, 694, 837, 1093
MboII GAAGA 2 cut(s) 149, 970
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 2 cut(s) 489, 852
MluCI AATT 7 cut(s) 27, 112, 422, 584, 859, 975, 1029
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 66, 171, 227, 428, 614, 663, 785, 905
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 1 cut(s) 1087
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 1 cut(s) 569
MspA1I CMGCKG 2 cut(s) 320, 464
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 383
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 2 cut(s) 153, 244
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 4 cut(s) 320, 464, 479, 1106
MwoI GCNNNNNNNGC 2 cut(s) 11, 150
NaeI GCCGGC 1 cut(s) 142
NdeII GATC 5 cut(s) 514, 547, 694, 837, 1093
NgoMIV GCCGGC 1 cut(s) 140
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 2 cut(s) 496, 773
NlaIV GGNNCC 2 cut(s) 380, 651
NmuCI GTSAC 1 cut(s) 979
NruI TCGCGA 1 cut(s) 479
NspI RCATGY 1 cut(s) 496
NspV TTCGAA 4 cut(s) 110, 536, 588, 866
PciI ACATGT 1 cut(s) 492
PctI GAATGC 2 cut(s) 153, 244
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 1087
PfeI GAWTC 4 cut(s) 107, 275, 402, 553
PkrI GCNGC 9 cut(s) 7, 215, 249, 319, 322, 745, 765, 902, 1048
PpuMI RGGWCCY 1 cut(s) 650
PscI ACATGT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 2 cut(s) 380, 651
PspPI GGNCC 3 cut(s) 379, 650, 914
PspPPI RGGWCCY 1 cut(s) 650
PstI CTGCAG 1 cut(s) 765
PstNI CAGNNNCTG 1 cut(s) 347
RruI TCGCGA 1 cut(s) 479
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 852
SacII CCGCGG 2 cut(s) 321, 465
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 9 cut(s) 6, 214, 248, 318, 321, 744, 764, 901, 1047
Sau3AI GATC 5 cut(s) 514, 547, 694, 837, 1093
Sau96I GGNCC 3 cut(s) 379, 650, 914
ScaI AGTACT 1 cut(s) 800
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 2 cut(s) 489, 852
SfaNI GCATC 3 cut(s) 710, 796, 983
SfcI CTRYAG 1 cut(s) 761
Sfr303I CCGCGG 2 cut(s) 321, 465
SfuI TTCGAA 4 cut(s) 110, 536, 588, 866
SgrBI CCGCGG 2 cut(s) 321, 465
SinI GGWCC 3 cut(s) 379, 650, 914
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
Sse9I AATT 7 cut(s) 27, 112, 422, 584, 859, 975, 1029
SsiI CCGC 9 cut(s) 138, 214, 318, 320, 462, 464, 903, 1097, 1106
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 428, 432
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 1 cut(s) 381
StyI CCWWGG 2 cut(s) 411, 616
TaaI ACNGT 2 cut(s) 921, 935
TaqI TCGA 7 cut(s) 110, 498, 536, 588, 701, 836, 866
TasI AATT 7 cut(s) 27, 112, 422, 584, 859, 975, 1029
TatI WGTACW 1 cut(s) 798
TauI GCSGC 3 cut(s) 216, 320, 323
TfiI GAWTC 4 cut(s) 107, 275, 402, 553
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 2 cut(s) 127, 1084
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 6 cut(s) 5, 247, 743, 763, 900, 1046
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 2 cut(s) 758, 1022
TspGWI ACGGA 2 cut(s) 268, 984
TspRI CASTG 2 cut(s) 127, 1084
VpaK11BI GGWCC 3 cut(s) 379, 650, 914
XapI RAATTY 4 cut(s) 27, 112, 584, 1029
XceI RCATGY 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 1087
XspI CTAG 2 cut(s) 428, 432
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.