AT1G62770

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004592
Physical Location & Seq
Reverse (-)
23245835 .. 23246949
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62770.1

Sequence Viewer

Length: 615 bp
ATGGAGCTTAAAAATACAATCTTTCTAGTCATACTTCTATCAATAACCATTCTCCAATCTTCATCTGCAACCCCAAACCGGTCGGAATCAGACCAGTTTATTGTGTCTTCATGCCAAACCACCCAATATCCATCACTATGCGTGCACACACTCTCTGCTTACGCCACCAAGATCCGCCACAACAATGACCAAGATCTCGCCCAGACTGCTCTCATCATCAGCTTGGCTCGAGCCAAATCCGTTACTATCTTCGTCGCCAAACTAACCAAAGAGACACCAAAATTTAAACGTAGGGAATATCTAGCAATCAAAGATTGCATCGAAGTGCTAGGCAATAGCGTGGACCGGTTAGCTCAATCGGTTAAGGAACTTGCTCGAGCTGGTCATGCTGTGGCTAGTGAGGACTTCATGTGGAAAATGAGTAACGTTCAAACATGGGTCAGTGCCGCTTTGACGGACGAGACAACGTGTCTAGATGGATTCTCAGAACGAGCCATGGGAGGCAAAGTCAAAAGACTGATTCGGTATAAGGTGGTTCACGTGGCTCAAGTCACTAGCAATGCTCTTGCTTTGGTTAATCAGTTTGCAGAGAAGCGAAGTGTGAAGTTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0065007 GO:0065009 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

204

Amino Acids

22.68

Weight (kDa)

9.43

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 191 4.2e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 175, 447
AclI AACGTT 1 cut(s) 426
AclWI GGATC 1 cut(s) 166
AcsI RAATTY 1 cut(s) 281
AcvI CACGTG 1 cut(s) 541
AfiI CCNNNNNNNGG 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 467
AgeI ACCGGT 2 cut(s) 78, 345
AgsI TTSAA 1 cut(s) 431
AhdI GACNNNNNGTC 1 cut(s) 468
AluBI AGCT 4 cut(s) 7, 222, 353, 380
AluI AGCT 4 cut(s) 7, 222, 353, 380
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 2 cut(s) 266, 455
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 1 cut(s) 166
Ama87I CYCGRG 2 cut(s) 228, 375
ApaLI GTGCAC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 281
AsiGI ACCGGT 2 cut(s) 78, 345
AspS9I GGNCC 1 cut(s) 343
AvaI CYCGRG 2 cut(s) 228, 375
AvaII GGWCC 1 cut(s) 343
BaeGI GKGCMC 1 cut(s) 147
BbrPI CACGTG 1 cut(s) 541
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 147
BccI CCATC 2 cut(s) 139, 470
BcoDI GTCTC 2 cut(s) 266, 455
BfaI CTAG 6 cut(s) 26, 302, 329, 396, 473, 555
BglII AGATCT 1 cut(s) 193
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 343
BmeRI GACNNNNNGTC 1 cut(s) 468
BmeT110I CYCGRG 2 cut(s) 228, 375
BmgT120I GGNCC 1 cut(s) 343
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 541
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 2 cut(s) 78, 345
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse118I RCCGGY 2 cut(s) 78, 345
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 1 cut(s) 94
BseSI GKGCMC 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 83
BshTI ACCGGT 2 cut(s) 78, 345
BsiEI CGRYCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 2 cut(s) 228, 375
BsiSI CCGG 2 cut(s) 79, 346
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 2 cut(s) 266, 455
BsoBI CYCGRG 2 cut(s) 228, 375
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 171, 193
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 2 cut(s) 175, 447
BspCNI CTCAG 1 cut(s) 497
BspPI GGATC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 565
BsrFI RCCGGY 2 cut(s) 78, 345
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 2 cut(s) 78, 345
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 2 cut(s) 171, 193
BssT1I CCWWGG 1 cut(s) 495
BstBAI YACGTR 1 cut(s) 541
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 1 cut(s) 484
BstDSI CCRYGG 1 cut(s) 495
BstKTI GATC 2 cut(s) 174, 196
BstMAI GTCTC 2 cut(s) 266, 455
BstMBI GATC 2 cut(s) 171, 193
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 2 cut(s) 206, 386
BstSLI GKGCMC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 171, 193
BstYI RGATCY 2 cut(s) 171, 193
BtgI CCRYGG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 143
Cfr10I RCCGGY 2 cut(s) 78, 345
Cfr13I GGNCC 1 cut(s) 343
CspAI ACCGGT 2 cut(s) 78, 345
CviAII CATG 5 cut(s) 111, 386, 409, 435, 496
CviJI RGCY 9 cut(s) 7, 222, 227, 233, 353, 380, 395, 494, 545
CviKI_1 RGCY 9 cut(s) 7, 222, 227, 233, 353, 380, 395, 494, 545
DdeI CTNAG 1 cut(s) 484
DpnI GATC 2 cut(s) 173, 195
DpnII GATC 2 cut(s) 171, 193
DraI TTTAAA 1 cut(s) 286
DriI GACNNNNNGTC 1 cut(s) 468
Eam1105I GACNNNNNGTC 1 cut(s) 468
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 343
Eco72I CACGTG 1 cut(s) 541
Eco88I CYCGRG 2 cut(s) 228, 375
EcoT14I CCWWGG 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 5 cut(s) 114, 389, 412, 438, 499
FaiI YATR 8 cut(s) 32, 112, 139, 387, 410, 436, 497, 528
FatI CATG 5 cut(s) 110, 385, 408, 434, 495
Fnu4HI GCNGC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 6 cut(s) 26, 302, 329, 396, 473, 555
GluI GCNGC 1 cut(s) 447
HapII CCGG 2 cut(s) 79, 346
Hin1II CATG 5 cut(s) 114, 389, 412, 438, 499
HinfI GANTC 3 cut(s) 86, 480, 520
HpaII CCGG 2 cut(s) 79, 346
Hpy166II GTNNAC 3 cut(s) 145, 343, 538
Hpy188I TCNGA 3 cut(s) 85, 91, 487
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 3 cut(s) 145, 343, 538
Hpy99I CGWCG 1 cut(s) 257
HpyCH4IV ACGT 4 cut(s) 289, 426, 467, 540
HpyCH4V TGCA 4 cut(s) 68, 145, 318, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 386
HpyF3I CTNAG 1 cut(s) 484
HpySE526I ACGT 4 cut(s) 289, 426, 467, 540
Hsp92II CATG 5 cut(s) 114, 389, 412, 438, 499
Kzo9I GATC 2 cut(s) 171, 193
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 92, 107, 215, 359, 366
LweI GCATC 1 cut(s) 327
MaeI CTAG 6 cut(s) 26, 302, 329, 396, 473, 555
MaeII ACGT 4 cut(s) 289, 426, 467, 540
MaeIII GTNAC 3 cut(s) 241, 422, 550
MalI GATC 2 cut(s) 173, 195
MboI GATC 2 cut(s) 171, 193
MboII GAAGA 3 cut(s) 51, 99, 241
MflI RGATCY 2 cut(s) 171, 193
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 2 cut(s) 394, 494
MseI TTAA 5 cut(s) 9, 285, 363, 576, 613
MslI CAYNNNNRTG 3 cut(s) 136, 183, 323
MspI CCGG 2 cut(s) 79, 346
MwoI GCNNNNNNNGC 2 cut(s) 206, 386
NcoI CCATGG 1 cut(s) 495
NdeII GATC 2 cut(s) 171, 193
NlaIII CATG 5 cut(s) 114, 389, 412, 438, 499
NmuCI GTSAC 1 cut(s) 550
PaeR7I CTCGAG 2 cut(s) 228, 375
PfeI GAWTC 3 cut(s) 86, 480, 520
PinAI ACCGGT 2 cut(s) 78, 345
PkrI GCNGC 1 cut(s) 448
PmaCI CACGTG 1 cut(s) 541
PmlI CACGTG 1 cut(s) 541
Ppu21I YACGTR 1 cut(s) 541
Psp1406I AACGTT 1 cut(s) 426
PspCI CACGTG 1 cut(s) 541
PspPI GGNCC 1 cut(s) 343
PspXI VCTCGAGB 2 cut(s) 228, 375
PsuI RGATCY 2 cut(s) 171, 193
RseI CAYNNNNRTG 3 cut(s) 136, 183, 323
SaqAI TTAA 5 cut(s) 9, 285, 363, 576, 613
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 2 cut(s) 171, 193
Sau96I GGNCC 1 cut(s) 343
SduI GDGCHC 1 cut(s) 147
SetI ASST 9 cut(s) 9, 224, 292, 355, 382, 429, 470, 534, 543
SfaNI GCATC 1 cut(s) 327
Sfr274I CTCGAG 2 cut(s) 228, 375
SinI GGWCC 1 cut(s) 343
SlaI CTCGAG 2 cut(s) 228, 375
SmiMI CAYNNNNRTG 3 cut(s) 136, 183, 323
SmlI CTYRAG 3 cut(s) 228, 375, 546
SmoI CTYRAG 3 cut(s) 228, 375, 546
Sse9I AATT 1 cut(s) 281
SsiI CCGC 2 cut(s) 175, 447
SspMI CTAG 6 cut(s) 26, 302, 329, 396, 473, 555
StyI CCWWGG 1 cut(s) 495
TaiI ACGT 4 cut(s) 292, 429, 470, 543
TaqI TCGA 3 cut(s) 229, 321, 376
TasI AATT 1 cut(s) 281
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 3 cut(s) 86, 480, 520
Tru1I TTAA 5 cut(s) 9, 285, 363, 576, 613
Tru9I TTAA 5 cut(s) 9, 285, 363, 576, 613
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 3 cut(s) 51, 99, 397
TspGWI ACGGA 2 cut(s) 229, 470
TspRI CASTG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 343
XapI RAATTY 1 cut(s) 281
XbaI TCTAGA 1 cut(s) 472
XhoI CTCGAG 2 cut(s) 228, 375
XspI CTAG 6 cut(s) 26, 302, 329, 396, 473, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.