Rroxscaffold_4G00283280

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004592
Physical Location & Seq
Forward (+)
5245750 .. 5247001
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283280.1

Sequence Viewer

Length: 663 bp
ATGCATATGCCAATACAAAAGGATCAGCTGTGTTCTAGATGGTTTTCTTGTAAGCGCTTTGGGAAGATGGATGAGGCACCTGGAATTCTTGGTAAGTTTTGGACTTCTAGTGGTGTACTAGGGTTCGATTGTTTTGAAAGGATAGAGAATGGGGCATTTTTGATATTTTCACAGCTGAAAGTAGGAGAGAGACCAACTCTGCTTCCACCACCACTGCCTCCAACTTCGTCAAAACCTCATGCAGCACCACCACCTACCCTGACCTCTGCGTTCAGTCCCCTCTCATCATTCTCCACCGCGATCCAGCAAAGCCCTCGCCAGCTGGCCCAGACCGCCCTCTCCGTCAGCCTCACCCGAGCCCAGTTCGCCAAGTCCTTCGTCACCAAGCTGACCAAGTTCAAGGGTTTGAGCCACAAGGAGTACGAGGCCATCGACGACTGCCTTGAGGAGATGGGTGACAGCGTCGACCGGCTGAGCAAGTCCGTGGACGAGCTGAAGAAGATGGGGAAGTCATCCAAGGGCCAGGATTTCATGTGGCACATGAGCAATGTTGAGACATGGGTCAGCGCTGCGTTGACTGACGACAACACTTGCCTTGATGGGTTCTCCGGGAAGGCCATGAATGGCAAGATCAAGGCCTCCATTAGAGCTCAAAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

220

Amino Acids

24.19

Weight (kDa)

8.95

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 94 - 215 9.3e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 465
AccII CGCG 1 cut(s) 299
AciI CCGC 2 cut(s) 297, 333
AclWI GGATC 2 cut(s) 30, 295
AcsI RAATTY 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 2 cut(s) 117, 422
AfeI AGCGCT 2 cut(s) 56, 568
AgsI TTSAA 2 cut(s) 137, 400
AjnI CCWGG 2 cut(s) 79, 522
AluBI AGCT 6 cut(s) 28, 175, 322, 388, 493, 650
AluI AGCT 6 cut(s) 28, 175, 322, 388, 493, 650
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 184, 548
AlwI GGATC 2 cut(s) 30, 295
Ama87I CYCGRG 1 cut(s) 354
Aor51HI AGCGCT 2 cut(s) 56, 568
AoxI GGCC 5 cut(s) 324, 426, 520, 615, 636
ApeKI GCWGC 2 cut(s) 242, 569
ApoI RAATTY 1 cut(s) 84
AspLEI GCGC 2 cut(s) 57, 569
AspS9I GGNCC 2 cut(s) 325, 520
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 3 cut(s) 343, 373, 467
AvaI CYCGRG 1 cut(s) 354
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 361, 652
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 2 cut(s) 254, 556
BccI CCATC 6 cut(s) 33, 61, 437, 445, 496, 593
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 81, 524
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 2 cut(s) 184, 548
BfaI CTAG 3 cut(s) 36, 108, 119
BfoI RGCGCY 2 cut(s) 58, 570
BisI GCNGC 2 cut(s) 243, 570
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 2 cut(s) 244, 571
Bme1390I CCNGG 3 cut(s) 81, 524, 610
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 325, 520
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 3 cut(s) 81, 524, 610
BmrI ACTGGG 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 355
BoxI GACNNNNGTC 1 cut(s) 560
BplI GAGNNNNNCTC 2 cut(s) 181, 213
Bpu1102I GCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 610
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 483, 516
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 553
BseBI CCWGG 2 cut(s) 81, 524
BseDI CCNNGG 2 cut(s) 483, 516
BseGI GGATG 2 cut(s) 76, 512
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 254, 556
Bsh1236I CGCG 1 cut(s) 299
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 5 cut(s) 326, 428, 522, 617, 638
BshNI GGYRCC 1 cut(s) 76
BsiEI CGRYCG 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 652
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 2 cut(s) 469, 609
BslFI GGGAC 1 cut(s) 261
BsmAI GTCTC 2 cut(s) 184, 548
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 5 cut(s) 326, 428, 522, 617, 638
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 361, 652
Bsp143I GATC 3 cut(s) 22, 300, 630
Bsp1720I GCTNAGC 1 cut(s) 473
BspACI CCGC 2 cut(s) 297, 333
BspANI GGCC 5 cut(s) 326, 428, 522, 617, 638
BspCNI CTCAG 1 cut(s) 465
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 2 cut(s) 30, 295
BspT107I GGYRCC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 483, 516
BssMI GATC 3 cut(s) 22, 300, 630
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 81, 524
BstC8I GCNNGC 2 cut(s) 320, 324
BstDEI CTNAG 1 cut(s) 473
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 2 cut(s) 76, 512
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 2 cut(s) 58, 570
BstHHI GCGC 2 cut(s) 57, 569
BstKTI GATC 3 cut(s) 25, 303, 633
BstMAI GTCTC 2 cut(s) 184, 548
BstMBI GATC 3 cut(s) 22, 300, 630
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 3 cut(s) 332, 365, 656
BstNI CCWGG 2 cut(s) 81, 524
BstPAI GACNNNNGTC 1 cut(s) 560
BstSCI CCNGG 3 cut(s) 79, 522, 608
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 254, 556
BsuRI GGCC 5 cut(s) 326, 428, 522, 617, 638
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 2 cut(s) 76, 512
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 212
Cac8I GCNNGC 2 cut(s) 320, 324
CfoI GCGC 2 cut(s) 57, 569
Cfr10I RCCGGY 1 cut(s) 468
Cfr13I GGNCC 2 cut(s) 325, 520
CseI GACGC 1 cut(s) 451
Csp6I GTAC 2 cut(s) 116, 421
CviAII CATG 5 cut(s) 239, 532, 541, 558, 619
CviQI GTAC 2 cut(s) 116, 421
DdeI CTNAG 1 cut(s) 473
DpnI GATC 3 cut(s) 24, 302, 632
DpnII GATC 3 cut(s) 22, 300, 630
Ecl136II GAGCTC 1 cut(s) 650
Eco130I CCWWGG 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 638
Eco24I GRGCYC 2 cut(s) 361, 652
Eco31I GGTCTC 1 cut(s) 184
Eco47III AGCGCT 2 cut(s) 56, 568
Eco53kI GAGCTC 1 cut(s) 650
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 79, 522
EcoT14I CCWWGG 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 361, 652
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 5 cut(s) 242, 535, 544, 561, 622
FaiI YATR 7 cut(s) 6, 8, 240, 533, 542, 559, 620
FaqI GGGAC 1 cut(s) 261
FatI CATG 5 cut(s) 238, 531, 540, 557, 618
FauNDI CATATG 1 cut(s) 6
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 243, 570
FokI GGATG 2 cut(s) 83, 499
FriOI GRGCYC 2 cut(s) 361, 652
Fsp4HI GCNGC 2 cut(s) 243, 570
FspBI CTAG 3 cut(s) 36, 108, 119
GlaI GCGC 2 cut(s) 56, 568
GluI GCNGC 2 cut(s) 243, 570
HaeII RGCGCY 2 cut(s) 58, 570
HaeIII GGCC 5 cut(s) 326, 428, 522, 617, 638
HapII CCGG 2 cut(s) 469, 609
HgaI GACGC 1 cut(s) 451
HhaI GCGC 2 cut(s) 57, 569
Hin1II CATG 5 cut(s) 242, 535, 544, 561, 622
Hin6I GCGC 2 cut(s) 55, 567
HinP1I GCGC 2 cut(s) 55, 567
HincII GTYRAC 2 cut(s) 466, 576
HindII GTYRAC 2 cut(s) 466, 576
HpaII CCGG 2 cut(s) 469, 609
HphI GGTGA 3 cut(s) 343, 373, 467
Hpy166II GTNNAC 4 cut(s) 116, 466, 487, 576
Hpy188III TCNNGA 1 cut(s) 36
Hpy8I GTNNAC 4 cut(s) 116, 466, 487, 576
Hpy99I CGWCG 2 cut(s) 437, 467
HpyAV CCTTC 2 cut(s) 385, 607
HpyCH4V TGCA 2 cut(s) 4, 242
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 365, 656
HpyF3I CTNAG 1 cut(s) 473
Hsp92II CATG 5 cut(s) 242, 535, 544, 561, 622
HspAI GCGC 2 cut(s) 55, 567
Kzo9I GATC 3 cut(s) 22, 300, 630
Lsp1109I GCAGC 2 cut(s) 254, 556
MaeI CTAG 3 cut(s) 36, 108, 119
MaeIII GTNAC 2 cut(s) 379, 455
MalI GATC 3 cut(s) 24, 302, 632
MboI GATC 3 cut(s) 22, 300, 630
MboII GAAGA 3 cut(s) 76, 508, 511
MhlI GDGCHC 2 cut(s) 361, 652
MluCI AATT 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 661
MspA1I CMGCKG 3 cut(s) 28, 175, 322
MspI CCGG 2 cut(s) 469, 609
MspR9I CCNGG 3 cut(s) 81, 524, 610
MvaI CCWGG 2 cut(s) 81, 524
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 332, 365, 656
NciI CCSGG 1 cut(s) 610
NdeI CATATG 1 cut(s) 6
NdeII GATC 3 cut(s) 22, 300, 630
NlaIII CATG 5 cut(s) 242, 535, 544, 561, 622
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 379, 455
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 1 cut(s) 608
PkrI GCNGC 2 cut(s) 244, 571
PshAI GACNNNNGTC 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 652
Psp6I CCWGG 2 cut(s) 79, 522
PspGI CCWGG 2 cut(s) 79, 522
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 2 cut(s) 325, 520
PsyI GACNNNGTC 1 cut(s) 461
PvuII CAGCTG 3 cut(s) 28, 175, 322
RsaI GTAC 2 cut(s) 117, 422
RsaNI GTAC 2 cut(s) 116, 421
SacI GAGCTC 1 cut(s) 652
SalI GTCGAC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 661
SatI GCNGC 2 cut(s) 243, 570
Sau3AI GATC 3 cut(s) 22, 300, 630
Sau96I GGNCC 2 cut(s) 325, 520
ScrFI CCNGG 3 cut(s) 81, 524, 610
SduI GDGCHC 2 cut(s) 361, 652
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 1 cut(s) 84
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 2 cut(s) 297, 333
SspMI CTAG 3 cut(s) 36, 108, 119
SstI GAGCTC 1 cut(s) 652
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 3 cut(s) 79, 522, 608
StyI CCWWGG 1 cut(s) 516
TaqI TCGA 3 cut(s) 126, 432, 465
TasI AATT 1 cut(s) 84
TatI WGTACW 1 cut(s) 115
Tru1I TTAA 1 cut(s) 661
Tru9I TTAA 1 cut(s) 661
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 379, 455
TseI GCWGC 2 cut(s) 242, 569
Tsp45I GTSAC 2 cut(s) 379, 455
TspDTI ATGAA 2 cut(s) 520, 635
TspGWI ACGGA 2 cut(s) 331, 472
TspRI CASTG 1 cut(s) 219
Tth111I GACNNNGTC 1 cut(s) 461
XapI RAATTY 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 3 cut(s) 36, 108, 119
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.