FvH4_5g00430

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004592
Physical Location & Seq
Reverse (-)
287880 .. 288503
624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00430.t1

Sequence Viewer

Length: 624 bp
ATGGCGAGACTAGGCTTTATTTCCTTACTAGTTCTTTTCCATGTTGTTTACCTGGTTGGTAAAGCAGACTCAACCCCTCCCAGACACTCAAGTTCCACTAAGTTCATCAAAGCTTCATGCAGAGCAACACAGTACCCAGCTCTGTGTGTGCAATGCCTTGCAGGTTATGCACGCTCGATTCGACAGAGTGAAAGACAGCTAGCTCAGACTGCTTTGGCAGTGAGCCTATCCCGGGTGCGAAAAGCTGAATCCTTTGTGACCAAGTTGACTAAAGTTAGGGGGATCAAGCCTAGGGAGTACAGAGCTGTGAAAGACTGCATAGAGAACATGGGGGATGGCGTGGACCAGATTGGCCAGTCGATGAGGGAGCTTGGACATACGGGTCGAGCCTTTGGTCAGGAGTTCATGTGGCACATGAGCAATGTGCAGACTTGGGTTAGTGCTGCACTCACTGATGAGACCACTTGTCTTGATGGGTTTTCAGGTCGTCTTATGGATGGAAATATCAAGGTTGCTATCAGAAGAAGGGTCAATGATGTTGCTCAGGTCACTAGCAACGCACTAGCTCTGGTTAATCGCTTTGCAACTAAACACAAGGCGGCTGCAGCTGCCAAAGATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

208

Amino Acids

22.79

Weight (kDa)

10.03

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 190 3e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 152
AciI CCGC 1 cut(s) 599
AclWI GGATC 2 cut(s) 290, 611
AcoI YGGCCR 1 cut(s) 352
AfaI GTAC 2 cut(s) 134, 299
AfiI CCNNNNNNNGG 1 cut(s) 232
AhdI GACNNNNNGTC 1 cut(s) 465
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 9 cut(s) 113, 140, 199, 203, 245, 305, 370, 566, 608
AluI AGCT 9 cut(s) 113, 140, 199, 203, 245, 305, 370, 566, 608
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 290, 611
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 4 cut(s) 443, 602, 605, 608
AspA2I CCTAGG 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 2 cut(s) 232, 233
AsuNHI GCTAGC 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 1 cut(s) 343
AvrII CCTAGG 1 cut(s) 290
BalI TGGCCA 1 cut(s) 354
BbvI GCAGC 4 cut(s) 430, 589, 595, 617
BccI CCATC 3 cut(s) 329, 467, 491
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 2 cut(s) 232, 233
BcoDI GTCTC 1 cut(s) 452
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 6 cut(s) 11, 29, 200, 291, 552, 563
BfmI CTRYAG 1 cut(s) 603
BfuAI ACCTGC 1 cut(s) 152
BisI GCNGC 5 cut(s) 444, 600, 603, 606, 609
BlnI CCTAGG 1 cut(s) 290
BlsI GCNGC 5 cut(s) 445, 601, 604, 607, 610
Bme1390I CCNGG 3 cut(s) 53, 232, 233
Bme18I GGWCC 1 cut(s) 343
BmeRI GACNNNNNGTC 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 343
BmrFI CCNGG 3 cut(s) 53, 232, 233
BmtI GCTAGC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 232, 233
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 231, 290
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 158, 427
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 231, 290
BseGI GGATG 2 cut(s) 340, 502
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 2 cut(s) 158, 427
BseMII CTCAG 2 cut(s) 218, 557
BseNI ACTGG 1 cut(s) 355
BseXI GCAGC 4 cut(s) 430, 589, 595, 617
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 2 cut(s) 429, 446
BshFI GGCC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 1 cut(s) 354
Bso31I GGTCTC 1 cut(s) 452
BsoBI CYCGRG 1 cut(s) 231
Bsp143I GATC 2 cut(s) 282, 616
BspACI CCGC 1 cut(s) 599
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 2 cut(s) 217, 556
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 1 cut(s) 152
BspOI GCTAGC 1 cut(s) 203
BspPI GGATC 2 cut(s) 290, 611
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 158, 427
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 231, 290
BssMI GATC 2 cut(s) 282, 616
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 172, 201
BstDEI CTNAG 4 cut(s) 99, 204, 543, 621
BstF5I GGATG 2 cut(s) 340, 502
BstKTI GATC 2 cut(s) 285, 619
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 282, 616
BstMWI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 51, 230, 231
BstSFI CTRYAG 1 cut(s) 603
BstV1I GCAGC 4 cut(s) 430, 589, 595, 617
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 2 cut(s) 340, 502
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 450
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 172, 201
Cfr13I GGNCC 1 cut(s) 343
Cfr9I CCCGGG 1 cut(s) 231
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 2 cut(s) 133, 298
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 5 cut(s) 41, 117, 328, 406, 415
CviQI GTAC 2 cut(s) 133, 298
DdeI CTNAG 4 cut(s) 99, 204, 543, 621
DpnI GATC 2 cut(s) 284, 618
DpnII GATC 2 cut(s) 282, 616
DrdI GACNNNNNNGTC 1 cut(s) 381
DriI GACNNNNNGTC 1 cut(s) 465
DseDI GACNNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 352
Eam1105I GACNNNNNGTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 5 cut(s) 44, 120, 331, 409, 418
FaiI YATR 9 cut(s) 42, 118, 168, 320, 329, 378, 407, 416, 494
FatI CATG 5 cut(s) 40, 116, 327, 405, 414
Fnu4HI GCNGC 5 cut(s) 444, 600, 603, 606, 609
FokI GGATG 2 cut(s) 347, 509
Fsp4HI GCNGC 5 cut(s) 444, 600, 603, 606, 609
FspBI CTAG 6 cut(s) 11, 29, 200, 291, 552, 563
GluI GCNGC 5 cut(s) 444, 600, 603, 606, 609
GsaI CCCAGC 1 cut(s) 140
HaeIII GGCC 1 cut(s) 354
HapII CCGG 1 cut(s) 232
Hin1II CATG 5 cut(s) 44, 120, 331, 409, 418
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 3 cut(s) 68, 178, 248
HpaII CCGG 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 49, 267, 343
Hpy188I TCNGA 2 cut(s) 207, 521
Hpy188III TCNNGA 2 cut(s) 398, 470
Hpy8I GTNNAC 3 cut(s) 49, 267, 343
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4V TGCA 9 cut(s) 120, 151, 161, 170, 318, 427, 446, 584, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
HpyF3I CTNAG 4 cut(s) 99, 204, 543, 621
Hsp92II CATG 5 cut(s) 44, 120, 331, 409, 418
Kzo9I GATC 2 cut(s) 282, 616
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 4 cut(s) 430, 589, 595, 617
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 6 cut(s) 11, 29, 200, 291, 552, 563
MaeIII GTNAC 2 cut(s) 256, 547
MalI GATC 2 cut(s) 284, 618
MboI GATC 2 cut(s) 282, 616
MboII GAAGA 1 cut(s) 534
MflI RGATCY 1 cut(s) 616
MlsI TGGCCA 1 cut(s) 354
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 2 cut(s) 87, 357
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
MseI TTAA 1 cut(s) 573
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 3 cut(s) 53, 232, 233
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
NciI CCSGG 2 cut(s) 232, 233
NdeII GATC 2 cut(s) 282, 616
NheI GCTAGC 1 cut(s) 199
NlaIII CATG 5 cut(s) 44, 120, 331, 409, 418
NmuCI GTSAC 2 cut(s) 256, 547
PcsI WCGNNNNNNNCGW 1 cut(s) 178
PfeI GAWTC 2 cut(s) 178, 248
PkrI GCNGC 5 cut(s) 445, 601, 604, 607, 610
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 1 cut(s) 51
PspPI GGNCC 1 cut(s) 343
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 134, 299
RsaNI GTAC 2 cut(s) 133, 298
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 5 cut(s) 444, 600, 603, 606, 609
Sau3AI GATC 2 cut(s) 282, 616
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 3 cut(s) 53, 232, 233
SexAI ACCWGGT 1 cut(s) 51
SfcI CTRYAG 1 cut(s) 603
SinI GGWCC 1 cut(s) 343
SmaI CCCGGG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SpeI ACTAGT 1 cut(s) 28
SsiI CCGC 1 cut(s) 599
SspMI CTAG 6 cut(s) 11, 29, 200, 291, 552, 563
StyD4I CCNGG 3 cut(s) 51, 230, 231
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 176, 181, 359, 385
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 2 cut(s) 178, 248
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 2 cut(s) 225, 457
TseFI GTSAC 2 cut(s) 256, 547
TseI GCWGC 4 cut(s) 443, 602, 605, 608
Tsp45I GTSAC 2 cut(s) 256, 547
TspDTI ATGAA 3 cut(s) 94, 105, 394
TspMI CCCGGG 1 cut(s) 231
TspRI CASTG 2 cut(s) 225, 457
VpaK11BI GGWCC 1 cut(s) 343
XmaI CCCGGG 1 cut(s) 231
XmaJI CCTAGG 1 cut(s) 290
XspI CTAG 6 cut(s) 11, 29, 200, 291, 552, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.