Rh7BG196600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004592
Physical Location & Seq
Forward (+)
16119318 .. 16119941
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG196600.1

Sequence Viewer

Length: 624 bp
ATGGCAAGACTTGGTCTGATTTCCTTACTAGTTCTCTTCCATGTTGTTTGCCTGGTTGGTAAAGCACACTCAACCCCTCCTAGACACTCAAATTCCACTAAATTCATCGAAGCTTCATGCAGAGCAACCCAGTATCCAGCTCTTTGTGTTCAATGCCTTGCAGGTTATGCACGCTCGATTCGACAGAGTGAAAGACAGCTAGCTCAGACTGCTTTGGCAGTGAGCCTATCCCGGGTGCGGAAAGCTGAATCCTTTGTGGCCAAGTTGACTAAAGTCAGGGGGATCAAGCCTAGGGAGTACAGCGCTGTGAAGGACTGCATAGAGAACATGGGGGATGGCGTGGACCGGCTTGGCCAGTCGGTGAGGGAGCTTGGACATACCTGTCGAGCCTTTGGTCAGGAGTTCATGTGGCACATGAGCAATGTGCAGACTTGGGTTAGTGCTGCACTCACTGATGAGACCACTTGTCTTGATGGGTTTTCAGGTCGTGTTATGGATGGAAATATCAAGGTTGCTATAAGAAGAAGGGTCAATAATGTTGCTCAGGTCACTAGCAACGCACTGGCTCTGGTTAATCGCTTTGCTGCTAAACACAGGGCGGCCGCAGCTGCCAAAGAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

207

Amino Acids

22.69

Weight (kDa)

9.99

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 190 1.5e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 152
AciI CCGC 3 cut(s) 238, 599, 603
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 3 cut(s) 258, 352, 600
AcsI RAATTY 2 cut(s) 91, 101
AfaI GTAC 1 cut(s) 299
AfeI AGCGCT 1 cut(s) 304
AfiI CCNNNNNNNGG 2 cut(s) 232, 237
AgsI TTSAA 1 cut(s) 152
AhdI GACNNNNNGTC 1 cut(s) 465
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 7 cut(s) 113, 140, 199, 203, 245, 370, 608
AluI AGCT 7 cut(s) 113, 140, 199, 203, 245, 370, 608
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 231
Aor51HI AGCGCT 1 cut(s) 304
AoxI GGCC 3 cut(s) 258, 352, 600
ApeKI GCWGC 4 cut(s) 443, 584, 605, 608
ApoI RAATTY 2 cut(s) 91, 101
AspA2I CCTAGG 1 cut(s) 290
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 2 cut(s) 232, 233
AsuHPI GGTGA 1 cut(s) 373
AsuNHI GCTAGC 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 1 cut(s) 343
AvrII CCTAGG 1 cut(s) 290
BalI TGGCCA 2 cut(s) 260, 354
BanII GRGCYC 1 cut(s) 621
BbvI GCAGC 4 cut(s) 430, 571, 595, 617
BccI CCATC 3 cut(s) 329, 467, 491
BciT130I CCWGG 1 cut(s) 53
BciVI GTATCC 1 cut(s) 144
BcnI CCSGG 2 cut(s) 232, 233
BcoDI GTCTC 1 cut(s) 452
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 6 cut(s) 29, 81, 200, 291, 552, 622
BfoI RGCGCY 1 cut(s) 306
BfuAI ACCTGC 1 cut(s) 152
BfuI GTATCC 1 cut(s) 144
BisI GCNGC 6 cut(s) 444, 585, 600, 603, 606, 609
BlnI CCTAGG 1 cut(s) 290
BlsI GCNGC 6 cut(s) 445, 586, 601, 604, 607, 610
Bme1390I CCNGG 3 cut(s) 53, 232, 233
Bme18I GGWCC 1 cut(s) 343
BmeRI GACNNNNNGTC 1 cut(s) 465
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 343
BmrFI CCNGG 3 cut(s) 53, 232, 233
BmrI ACTGGG 1 cut(s) 124
BmtI GCTAGC 1 cut(s) 203
BmuI ACTGGG 1 cut(s) 124
BoxI GACNNNNGTC 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 543
BpuMI CCSGG 2 cut(s) 232, 233
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 231, 290
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 237
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 3 cut(s) 130, 355, 567
Bse3DI GCAATG 1 cut(s) 427
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 231, 290
BseGI GGATG 2 cut(s) 340, 502
BseLI CCNNNNNNNGG 2 cut(s) 232, 237
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 218, 557
BseNI ACTGG 3 cut(s) 130, 355, 567
BseX3I CGGCCG 1 cut(s) 600
BseXI GCAGC 4 cut(s) 430, 571, 595, 617
BsgI GTGCAG 2 cut(s) 429, 446
Bsh1285I CGRYCG 1 cut(s) 603
BshFI GGCC 3 cut(s) 260, 354, 602
BsiEI CGRYCG 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 2 cut(s) 232, 346
BslI CCNNNNNNNGG 2 cut(s) 232, 237
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 3 cut(s) 260, 354, 602
Bso31I GGTCTC 1 cut(s) 452
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 1 cut(s) 282
BspACI CCGC 3 cut(s) 238, 599, 603
BspANI GGCC 3 cut(s) 260, 354, 602
BspCNI CTCAG 2 cut(s) 217, 556
BspMI ACCTGC 1 cut(s) 152
BspOI GCTAGC 1 cut(s) 203
BspPI GGATC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 345
BsrI ACTGG 3 cut(s) 130, 355, 567
BssAI RCCGGY 1 cut(s) 345
BssECI CCNNGG 2 cut(s) 231, 290
BssMI GATC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 53
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 172, 201
BstDEI CTNAG 2 cut(s) 204, 543
BstF5I GGATG 2 cut(s) 340, 502
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 1 cut(s) 285
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 282
BstMCI CGRYCG 1 cut(s) 603
BstMWI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
BstNI CCWGG 1 cut(s) 53
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 51, 230, 231
BstV1I GCAGC 4 cut(s) 430, 571, 595, 617
BstZI CGGCCG 1 cut(s) 600
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 3 cut(s) 260, 354, 602
BtsCI GGATG 2 cut(s) 340, 502
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 3 cut(s) 225, 450, 560
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 172, 201
CciNI GCGGCCGC 1 cut(s) 600
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 345
Cfr13I GGNCC 1 cut(s) 343
Cfr9I CCCGGG 1 cut(s) 231
Csp6I GTAC 1 cut(s) 298
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 5 cut(s) 41, 117, 328, 406, 415
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 2 cut(s) 204, 543
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
DrdI GACNNNNNNGTC 1 cut(s) 381
DriI GACNNNNNGTC 1 cut(s) 465
DseDI GACNNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 3 cut(s) 258, 352, 600
EagI CGGCCG 1 cut(s) 600
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 343
Eco47III AGCGCT 1 cut(s) 304
Eco52I CGGCCG 1 cut(s) 600
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 5 cut(s) 44, 120, 331, 409, 418
FatI CATG 5 cut(s) 40, 116, 327, 405, 414
Fnu4HI GCNGC 6 cut(s) 444, 585, 600, 603, 606, 609
FokI GGATG 2 cut(s) 347, 509
FriOI GRGCYC 1 cut(s) 621
Fsp4HI GCNGC 6 cut(s) 444, 585, 600, 603, 606, 609
FspBI CTAG 6 cut(s) 29, 81, 200, 291, 552, 622
GlaI GCGC 1 cut(s) 304
GluI GCNGC 6 cut(s) 444, 585, 600, 603, 606, 609
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 3 cut(s) 260, 354, 602
HapII CCGG 2 cut(s) 232, 346
HhaI GCGC 1 cut(s) 305
Hin1II CATG 5 cut(s) 44, 120, 331, 409, 418
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 2 cut(s) 178, 248
HpaII CCGG 2 cut(s) 232, 346
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 2 cut(s) 267, 343
Hpy188I TCNGA 2 cut(s) 18, 207
Hpy188III TCNNGA 2 cut(s) 398, 470
Hpy8I GTNNAC 2 cut(s) 267, 343
HpyAV CCTTC 2 cut(s) 304, 519
HpyCH4V TGCA 6 cut(s) 120, 161, 170, 318, 427, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
HpyF3I CTNAG 2 cut(s) 204, 543
Hsp92II CATG 5 cut(s) 44, 120, 331, 409, 418
HspAI GCGC 1 cut(s) 303
Kzo9I GATC 1 cut(s) 282
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 4 cut(s) 430, 571, 595, 617
MaeI CTAG 6 cut(s) 29, 81, 200, 291, 552, 622
MaeIII GTNAC 1 cut(s) 547
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 2 cut(s) 28, 534
MhlI GDGCHC 1 cut(s) 621
MlsI TGGCCA 2 cut(s) 260, 354
MluCI AATT 2 cut(s) 91, 101
MluNI TGGCCA 2 cut(s) 260, 354
MnlI CCTC 2 cut(s) 87, 357
Mox20I TGGCCA 2 cut(s) 260, 354
MscI TGGCCA 2 cut(s) 260, 354
MseI TTAA 1 cut(s) 573
Msp20I TGGCCA 2 cut(s) 260, 354
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 2 cut(s) 232, 346
MspR9I CCNGG 3 cut(s) 53, 232, 233
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 167, 209, 605, 608
NciI CCSGG 2 cut(s) 232, 233
NdeII GATC 1 cut(s) 282
NheI GCTAGC 1 cut(s) 199
NlaIII CATG 5 cut(s) 44, 120, 331, 409, 418
NmuCI GTSAC 1 cut(s) 547
NotI GCGGCCGC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 178
PfeI GAWTC 2 cut(s) 178, 248
PflFI GACNNNGTC 1 cut(s) 12
PkrI GCNGC 6 cut(s) 445, 586, 601, 604, 607, 610
PshAI GACNNNNGTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspPI GGNCC 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 6 cut(s) 444, 585, 600, 603, 606, 609
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 53, 232, 233
SduI GDGCHC 1 cut(s) 621
SinI GGWCC 1 cut(s) 343
SmaI CCCGGG 1 cut(s) 233
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 2 cut(s) 91, 101
SsiI CCGC 3 cut(s) 238, 599, 603
SspMI CTAG 6 cut(s) 29, 81, 200, 291, 552, 622
StyD4I CCNGG 3 cut(s) 51, 230, 231
StyI CCWWGG 1 cut(s) 290
TaqI TCGA 4 cut(s) 108, 176, 181, 385
TasI AATT 2 cut(s) 91, 101
TatI WGTACW 1 cut(s) 297
TauI GCSGC 2 cut(s) 602, 605
TfiI GAWTC 2 cut(s) 178, 248
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 3 cut(s) 225, 457, 567
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 4 cut(s) 443, 584, 605, 608
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 94, 105, 394
TspMI CCCGGG 1 cut(s) 231
TspRI CASTG 3 cut(s) 225, 457, 567
Tth111I GACNNNGTC 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 343
XapI RAATTY 2 cut(s) 91, 101
XmaI CCCGGG 1 cut(s) 231
XmaJI CCTAGG 1 cut(s) 290
XspI CTAG 6 cut(s) 29, 81, 200, 291, 552, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.