RLG00000026718

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004592
Physical Location & Seq
Forward (+)
5281666 .. 5282498
833 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026718

Sequence Viewer

Length: 627 bp
ATGGCTAGATCCTTTATTCTCTTTCTACTTCTTGCCCTCCTCGCCCTCACAGCCAACTCTGCTTCCACCACCACTGCATCCAACTTCGTCAAAACCTCATGCAGCACCACCACCTACCCTGACCTCTGCGTTCAGTCCCTCTCATCATTCTCCACCGTGATCCAGCAAAGCCCTCGCCAGCTGGCCCAGACCGCCCTCTCCGTCAGCCTCACGCGAGCCCAGTTCGCCAAGTCCTTCGTCACCAAGCTGACCAAGTTCAAGGGTTTGAGCCACAAGGAGTACGAGGCCATCGACGACTGCCTTGAGGAGATGGGCGACAGCGTCGACCGGCTGAGCAAGTCGGTGGACGAGCTGAAGAAGATGGGGAAGTCATCCAAGGGCCAGGATTTCTTATGGCATATGAGCAATGTCGAGACTTGGGTCAGCGCTGCGTTGACTGACGACAACACTTGCCTTGATGGGTTCTCTGGGAAGGCCATGAATGGCAAGATCAAGGCCTCCATTAGAGCTCAAGTGCTTAATGTTGCCAAGTATCCTAGTTTTGGTACTTTAATTAAGGTGGGAAACTACACAAATATAGATCGATCGTCTTCACGCAATAGATACTACCCAGCTAGATCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

209

Amino Acids

22.83

Weight (kDa)

9.37

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 170 3e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 214
AciI CCGC 1 cut(s) 192
AclWI GGATC 2 cut(s) 3, 154
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 2 cut(s) 281, 547
AfeI AGCGCT 1 cut(s) 427
AfiI CCNNNNNNNGG 1 cut(s) 542
AgsI TTSAA 1 cut(s) 259
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 5 cut(s) 181, 247, 352, 509, 614
AluI AGCT 5 cut(s) 181, 247, 352, 509, 614
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 2 cut(s) 3, 154
Aor51HI AGCGCT 1 cut(s) 427
AoxI GGCC 5 cut(s) 183, 285, 379, 474, 495
ApeKI GCWGC 2 cut(s) 102, 428
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 2 cut(s) 184, 379
AsuHPI GGTGA 1 cut(s) 232
BanII GRGCYC 2 cut(s) 220, 511
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 114, 415
BccI CCATC 4 cut(s) 296, 304, 355, 452
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 383
BciVI GTATCC 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 3 cut(s) 6, 537, 615
BfoI RGCGCY 1 cut(s) 429
BfuI GTATCC 1 cut(s) 543
BglII AGATCT 1 cut(s) 617
BisI GCNGC 2 cut(s) 103, 429
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 104, 430
Bme1390I CCNGG 1 cut(s) 383
BmgT120I GGNCC 2 cut(s) 184, 379
BmrFI CCNGG 1 cut(s) 383
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 323, 495
Bsa29I ATCGAT 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 375
Bsc4I CCNNNNNNNGG 1 cut(s) 542
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 583
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 2 cut(s) 77, 371
BseLI CCNNNNNNNGG 1 cut(s) 542
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 2 cut(s) 29, 320
BseXI GCAGC 2 cut(s) 114, 415
BseYI CCCAGC 1 cut(s) 610
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 2 cut(s) 328, 587
BshFI GGCC 5 cut(s) 185, 287, 381, 476, 497
BshVI ATCGAT 1 cut(s) 583
BsiEI CGRYCG 2 cut(s) 328, 587
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 328
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 1 cut(s) 542
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 5 cut(s) 185, 287, 381, 476, 497
Bsp1286I GDGCHC 2 cut(s) 220, 511
Bsp143I GATC 6 cut(s) 8, 159, 489, 580, 584, 617
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 1 cut(s) 192
BspANI GGCC 5 cut(s) 185, 287, 381, 476, 497
BspCNI CTCAG 1 cut(s) 324
BspDI ATCGAT 1 cut(s) 583
BspFNI CGCG 1 cut(s) 214
BspPI GGATC 2 cut(s) 3, 154
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 6 cut(s) 8, 159, 489, 580, 584, 617
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 179, 183, 216
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 77, 371
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 6 cut(s) 11, 162, 492, 583, 587, 620
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 6 cut(s) 8, 159, 489, 580, 584, 617
BstMCI CGRYCG 2 cut(s) 328, 587
BstMWI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
BstNI CCWGG 1 cut(s) 383
BstPAI GACNNNNGTC 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 381
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 114, 415
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 8, 617
BstYI RGATCY 2 cut(s) 8, 617
Bsu15I ATCGAT 1 cut(s) 583
BsuI GTATCC 1 cut(s) 543
BsuRI GGCC 5 cut(s) 185, 287, 381, 476, 497
BsuTUI ATCGAT 1 cut(s) 583
BtsCI GGATG 2 cut(s) 77, 371
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 3 cut(s) 179, 183, 216
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 184, 379
ClaI ATCGAT 1 cut(s) 583
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 280, 546
CviAII CATG 2 cut(s) 99, 478
CviQI GTAC 2 cut(s) 280, 546
DdeI CTNAG 1 cut(s) 332
DpnI GATC 6 cut(s) 10, 161, 491, 582, 586, 619
DpnII GATC 6 cut(s) 8, 159, 489, 580, 584, 617
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 1 cut(s) 497
Eco24I GRGCYC 2 cut(s) 220, 511
Eco47III AGCGCT 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 2 cut(s) 220, 511
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 2 cut(s) 102, 481
FaiI YATR 6 cut(s) 100, 394, 399, 401, 479, 578
FaqI GGGAC 1 cut(s) 121
FatI CATG 2 cut(s) 98, 477
FauNDI CATATG 1 cut(s) 399
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 103, 429
FokI GGATG 2 cut(s) 64, 358
FriOI GRGCYC 2 cut(s) 220, 511
Fsp4HI GCNGC 2 cut(s) 103, 429
FspBI CTAG 3 cut(s) 6, 537, 615
GlaI GCGC 1 cut(s) 427
GluI GCNGC 2 cut(s) 103, 429
GsaI CCCAGC 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 5 cut(s) 185, 287, 381, 476, 497
HapII CCGG 1 cut(s) 328
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 428
Hin1II CATG 2 cut(s) 102, 481
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 2 cut(s) 325, 435
HindII GTYRAC 2 cut(s) 325, 435
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 325, 346, 435
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 3 cut(s) 325, 346, 435
Hpy99I CGWCG 2 cut(s) 296, 326
HpyAV CCTTC 2 cut(s) 244, 466
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 2 cut(s) 77, 102
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 2 cut(s) 102, 481
HspAI GCGC 1 cut(s) 426
Kzo9I GATC 6 cut(s) 8, 159, 489, 580, 584, 617
Lsp1109I GCAGC 2 cut(s) 114, 415
LweI GCATC 1 cut(s) 86
MaeI CTAG 3 cut(s) 6, 537, 615
MaeIII GTNAC 1 cut(s) 238
MalI GATC 6 cut(s) 10, 161, 491, 582, 586, 619
MboI GATC 6 cut(s) 8, 159, 489, 580, 584, 617
MboII GAAGA 3 cut(s) 367, 370, 582
MflI RGATCY 2 cut(s) 8, 617
MhlI GDGCHC 2 cut(s) 220, 511
MluCI AATT 1 cut(s) 552
MmeI TCCRAC 1 cut(s) 105
MseI TTAA 3 cut(s) 519, 551, 555
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 383
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
NdeI CATATG 1 cut(s) 399
NdeII GATC 6 cut(s) 8, 159, 489, 580, 584, 617
NlaIII CATG 2 cut(s) 102, 481
NmuCI GTSAC 1 cut(s) 238
PacI TTAATTAA 1 cut(s) 555
PceI AGGCCT 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PflFI GACNNNGTC 1 cut(s) 320
PkrI GCNGC 2 cut(s) 104, 430
Ple19I CGATCG 1 cut(s) 587
PshAI GACNNNNGTC 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 1 cut(s) 381
PspPI GGNCC 2 cut(s) 184, 379
PsuI RGATCY 2 cut(s) 8, 617
PsyI GACNNNGTC 1 cut(s) 320
PvuI CGATCG 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 2 cut(s) 281, 547
RsaNI GTAC 2 cut(s) 280, 546
SacI GAGCTC 1 cut(s) 511
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 3 cut(s) 519, 551, 555
SatI GCNGC 2 cut(s) 103, 429
Sau3AI GATC 6 cut(s) 8, 159, 489, 580, 584, 617
Sau96I GGNCC 2 cut(s) 184, 379
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 2 cut(s) 220, 511
SetI ASST 9 cut(s) 98, 116, 126, 183, 249, 354, 511, 561, 616
SfaNI GCATC 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 302, 510
SmoI CTYRAG 2 cut(s) 302, 510
Sse9I AATT 1 cut(s) 552
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 1 cut(s) 192
SspMI CTAG 3 cut(s) 6, 537, 615
SstI GAGCTC 1 cut(s) 511
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 381
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 4 cut(s) 291, 324, 411, 583
TasI AATT 1 cut(s) 552
Tru1I TTAA 3 cut(s) 519, 551, 555
Tru9I TTAA 3 cut(s) 519, 551, 555
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 102, 428
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 3 cut(s) 6, 537, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.