AT4G12390

Pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004592
Physical Location & Seq
Forward (+)
7336330 .. 7338180
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12390.1

Sequence Viewer

Length: 621 bp
ATGGAACCAAAGCTAACCCATCTTTGTTATTGCCTTCTACTTTTTCTTCCACTACTCTGCCAATCCACCATAGCCAAACCTTCATCATCACCAAACCCTAGCAGCAGCATCAACTTCATTGTCTCATCATGCCGCGTCACGCGTTACCAAACTCTCTGCGTCAAATGCCTTGCCGCTTTTGCAGACAAAATCCGCCGCAACGAAAACCAGTTAGCTCAAACCGCTTTAGCCGTTACTCTAGTCCGGGTTCAGTCCACGACAATCTATGTAGGGAAGCTGACTAAAGCTAGGAGAATCAAAAGGAGAGAGTACTTAGCCGTGAAGGATTGTGTTGAAAATCTTGGAGACGGTTTAGAGATGTTGGCTCAGTCGATGAGGGAGTTGAAGCAAGTTGGTCGATCTGGTCGTGATCGAGACGAGTTCTTGTGGAGACTAAGTAACGTTGAGACTTGGGTTAGTGCTGCCTTAACGGATGAGACAACGTGTCTGGATGGGTTCGATGGAAAGGTTATGGACGGTGTGGTAAAATCAGCGATTAGAAGAAGAGTGGTTCATGTGGCTCGAGTTACTAGTAATGCATTGGCTCTTGTAAACCGGTTCGCGGCTAGGCATAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

206

Amino Acids

23.08

Weight (kDa)

9.78

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 196 4.5e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 135, 142, 602
AciI CCGC 6 cut(s) 133, 174, 193, 196, 222, 602
AclI AACGTT 1 cut(s) 441
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 601
AflIII ACRYGT 2 cut(s) 140, 482
AgeI ACCGGT 1 cut(s) 594
AgsI TTSAA 2 cut(s) 335, 385
AhdI GACNNNNNGTC 1 cut(s) 483
AhlI ACTAGT 1 cut(s) 569
AluBI AGCT 4 cut(s) 13, 215, 277, 287
AluI AGCT 4 cut(s) 13, 215, 277, 287
Alw26I GTCTC 6 cut(s) 127, 339, 408, 424, 440, 470
Ama87I CYCGRG 1 cut(s) 561
ApeKI GCWGC 3 cut(s) 102, 105, 461
AsiGI ACCGGT 1 cut(s) 594
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 561
BbvI GCAGC 3 cut(s) 114, 117, 448
BccI CCATC 3 cut(s) 27, 485, 494
BceAI ACGGC 2 cut(s) 215, 302
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 6 cut(s) 127, 339, 408, 424, 440, 470
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 5 cut(s) 99, 239, 288, 570, 606
BisI GCNGC 7 cut(s) 103, 106, 133, 174, 196, 462, 603
BlsI GCNGC 7 cut(s) 104, 107, 134, 175, 197, 463, 604
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 245
BmeRI GACNNNNNGTC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 561
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 245
BsaWI WCCGGW 1 cut(s) 594
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 601
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 1 cut(s) 208
BseGI GGATG 2 cut(s) 478, 496
BseLI CCNNNNNNNGG 1 cut(s) 601
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 208
BseXI GCAGC 3 cut(s) 114, 117, 448
Bsh1236I CGCG 3 cut(s) 135, 142, 602
BshTI ACCGGT 1 cut(s) 594
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 2 cut(s) 244, 595
BslI CCNNNNNNNGG 1 cut(s) 601
BsmAI GTCTC 6 cut(s) 127, 339, 408, 424, 440, 470
BsmBI CGTCTC 2 cut(s) 339, 408
BsoBI CYCGRG 1 cut(s) 561
Bsp143I GATC 2 cut(s) 398, 409
BspACI CCGC 6 cut(s) 133, 174, 193, 196, 222, 602
BspCNI CTCAG 1 cut(s) 379
BspFNI CGCG 3 cut(s) 135, 142, 602
BspLI GGNNCC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 594
BssMI GATC 2 cut(s) 398, 409
Bst4CI ACNGT 2 cut(s) 350, 518
Bst6I CTCTTC 1 cut(s) 538
BstDEI CTNAG 3 cut(s) 313, 366, 434
BstF5I GGATG 2 cut(s) 478, 496
BstFNI CGCG 3 cut(s) 135, 142, 602
BstKTI GATC 2 cut(s) 401, 412
BstMAI GTCTC 6 cut(s) 127, 339, 408, 424, 440, 470
BstMBI GATC 2 cut(s) 398, 409
BstMWI GCNNNNNNNGC 3 cut(s) 165, 179, 221
BstSCI CCNGG 1 cut(s) 243
BstUI CGCG 3 cut(s) 135, 142, 602
BstV1I GCAGC 3 cut(s) 114, 117, 448
BtsCI GGATG 2 cut(s) 478, 496
Cfr10I RCCGGY 1 cut(s) 594
CseI GACGC 2 cut(s) 124, 148
Csp6I GTAC 1 cut(s) 310
CspAI ACCGGT 1 cut(s) 594
CviAII CATG 2 cut(s) 129, 554
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 3 cut(s) 313, 366, 434
DpnI GATC 2 cut(s) 400, 411
DpnII GATC 2 cut(s) 398, 409
DriI GACNNNNNGTC 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 538
Eam1105I GACNNNNNGTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 538
EciI GGCGGA 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 580
Esp3I CGTCTC 2 cut(s) 339, 408
FaeI CATG 2 cut(s) 132, 557
FaiI YATR 7 cut(s) 71, 130, 267, 512, 555, 612, 619
FatI CATG 2 cut(s) 128, 553
Fnu4HI GCNGC 7 cut(s) 103, 106, 133, 174, 196, 462, 603
FokI GGATG 2 cut(s) 485, 503
Fsp4HI GCNGC 7 cut(s) 103, 106, 133, 174, 196, 462, 603
FspBI CTAG 5 cut(s) 99, 239, 288, 570, 606
GluI GCNGC 7 cut(s) 103, 106, 133, 174, 196, 462, 603
HapII CCGG 2 cut(s) 244, 595
HgaI GACGC 2 cut(s) 124, 148
Hin1II CATG 2 cut(s) 132, 557
HinfI GANTC 1 cut(s) 294
HpaII CCGG 2 cut(s) 244, 595
HphI GGTGA 1 cut(s) 81
Hpy166II GTNNAC 2 cut(s) 255, 592
Hpy188III TCNNGA 3 cut(s) 407, 413, 488
Hpy8I GTNNAC 2 cut(s) 255, 592
HpyAV CCTTC 3 cut(s) 44, 90, 316
HpyCH4III ACNGT 2 cut(s) 350, 518
HpyCH4IV ACGT 2 cut(s) 441, 482
HpyCH4V TGCA 2 cut(s) 182, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 179, 221
HpyF3I CTNAG 3 cut(s) 313, 366, 434
HpySE526I ACGT 2 cut(s) 441, 482
Hsp92II CATG 2 cut(s) 132, 557
Kzo9I GATC 2 cut(s) 398, 409
LpnPI CCDG 5 cut(s) 221, 257, 387, 473, 608
Lsp1109I GCAGC 3 cut(s) 114, 117, 448
LweI GCATC 1 cut(s) 117
MaeI CTAG 5 cut(s) 99, 239, 288, 570, 606
MaeII ACGT 2 cut(s) 441, 482
MaeIII GTNAC 5 cut(s) 136, 143, 232, 437, 565
MalI GATC 2 cut(s) 400, 411
MboI GATC 2 cut(s) 398, 409
MboII GAAGA 3 cut(s) 38, 552, 555
MluI ACGCGT 1 cut(s) 140
MnlI CCTC 1 cut(s) 369
Mph1103I ATGCAT 1 cut(s) 580
MseI TTAA 1 cut(s) 467
MspI CCGG 2 cut(s) 244, 595
MspR9I CCNGG 1 cut(s) 245
MvnI CGCG 3 cut(s) 135, 142, 602
MwoI GCNNNNNNNGC 3 cut(s) 165, 179, 221
NciI CCSGG 1 cut(s) 245
NdeII GATC 2 cut(s) 398, 409
NlaIII CATG 2 cut(s) 132, 557
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 580
PaeR7I CTCGAG 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 403
PfeI GAWTC 1 cut(s) 294
PinAI ACCGGT 1 cut(s) 594
PkrI GCNGC 7 cut(s) 104, 107, 134, 175, 197, 463, 604
Psp1406I AACGTT 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 561
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 7 cut(s) 103, 106, 133, 174, 196, 462, 603
Sau3AI GATC 2 cut(s) 398, 409
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 1 cut(s) 245
SetI ASST 8 cut(s) 15, 82, 217, 279, 289, 444, 485, 510
SfaNI GCATC 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 561
SlaI CTCGAG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SpeI ACTAGT 1 cut(s) 569
SsiI CCGC 6 cut(s) 133, 174, 193, 196, 222, 602
SspMI CTAG 5 cut(s) 99, 239, 288, 570, 606
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 350, 518
TaiI ACGT 2 cut(s) 444, 485
TaqI TCGA 5 cut(s) 371, 397, 412, 498, 562
TatI WGTACW 1 cut(s) 309
TauI GCSGC 4 cut(s) 135, 176, 198, 605
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 3 cut(s) 102, 105, 461
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 72, 106, 542
TspGWI ACGGA 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 561
XspI CTAG 5 cut(s) 99, 239, 288, 570, 606
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.