MD07G1255800.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004592
Physical Location & Seq
Reverse (-)
32284234 .. 32284878
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1255800.v1.1.491

Sequence Viewer

Length: 645 bp
ATGCACAGATCTCAACCTTTCCCAAATCCACTCCCACTCCAACTCCAACTTTCTTCAATGGCTAAAGCCTCAATTGTCTTGCTTCTTCTTCTCCCCATTGTCTACTTTGTTTCCACAGCCAAATCTTCATCCACCACAACTCCTACGAACTTCATCAGAGACTCATGCAGCGCAACCACCTACCCAGAAGTCTGCATCAAATCCCTCTCTTCCTACGCCAGTTCAATCAAACAAAGCCCCCGCCAGCTGGCCCAGGCCGCCTTGTCCGTCAGCCTAACAAAAGCACAGAGCGCCAAAACATTCGTGACCAAGTTGGCCAAGTTTAAGGGACTAAAATCCAGGGAGTACGGAGCCATCAAAGACTGCATGGAGGAGATAGGGGACACCGTGGATAGGCTGAGCAAGTCCGTACAGGAGCTGAAGAACATGGGGAAATCCAAGGGTCAGGATTTCTTGTGGCACATGAGCAACGTGGAGACTTGGGTCAGTGCTGCTTTGACTGATGATAACACGTGTCTTGATGGGTTCTCCGGGAAGACATTGAACGGAAAGATCAAGTCGGCCGTTAGAGCTCAGGTGCTTAATGTTGCTCAGTGCACTAGTAATGCACTCTCCTTGTGCAACCGGTTTGCGGGCAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

215

Amino Acids

23.26

Weight (kDa)

9.57

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 50 - 206 2.1e-41 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 241, 258, 632
AcoI YGGCCR 2 cut(s) 315, 561
AcuI CTGAAG 1 cut(s) 440
AcvI CACGTG 1 cut(s) 513
AfaI GTAC 2 cut(s) 347, 411
AfiI CCNNNNNNNGG 3 cut(s) 247, 393, 631
AflIII ACRYGT 2 cut(s) 510, 512
AgeI ACCGGT 1 cut(s) 624
AgsI TTSAA 3 cut(s) 57, 225, 544
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 2 cut(s) 252, 338
AluBI AGCT 3 cut(s) 247, 418, 572
AluI AGCT 3 cut(s) 247, 418, 572
Alw21I GWGCWC 2 cut(s) 574, 599
Alw26I GTCTC 2 cut(s) 153, 470
Alw44I GTGCAC 1 cut(s) 595
AlwNI CAGNNNCTG 1 cut(s) 418
AoxI GGCC 4 cut(s) 249, 255, 315, 561
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 2 cut(s) 168, 491
AsiGI ACCGGT 1 cut(s) 624
AspLEI GCGC 2 cut(s) 173, 293
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 317
BanII GRGCYC 1 cut(s) 574
BbrPI CACGTG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 2 cut(s) 574, 599
BbvI GCAGC 2 cut(s) 180, 478
BccI CCATC 2 cut(s) 362, 515
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 2 cut(s) 254, 340
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 153, 470
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 294
BglII AGATCT 1 cut(s) 8
BisI GCNGC 3 cut(s) 169, 258, 492
BlpI GCTNAGC 1 cut(s) 398
BlsI GCNGC 3 cut(s) 170, 259, 493
Bme1390I CCNGG 3 cut(s) 254, 340, 532
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 3 cut(s) 254, 340, 532
BmsI GCATC 1 cut(s) 204
BoxI GACNNNNGTC 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 542
Bpu10I CCTNAGC 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 532
BsaAI YACGTR 1 cut(s) 513
BsaJI CCNNGG 4 cut(s) 252, 339, 387, 438
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 6 cut(s) 21, 27, 51, 57, 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 393, 631
Bse118I RCCGGY 1 cut(s) 624
Bse1I ACTGG 1 cut(s) 219
BseBI CCWGG 2 cut(s) 254, 340
BseDI CCNNGG 4 cut(s) 252, 339, 387, 438
BseGI GGATG 1 cut(s) 128
BseLI CCNNNNNNNGG 3 cut(s) 247, 393, 631
BseMII CTCAG 3 cut(s) 389, 587, 605
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 561
BseXI GCAGC 2 cut(s) 180, 478
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 4 cut(s) 251, 257, 317, 563
BshTI ACCGGT 1 cut(s) 624
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 574, 599
BsiSI CCGG 2 cut(s) 531, 625
BslFI GGGAC 2 cut(s) 342, 395
BslI CCNNNNNNNGG 3 cut(s) 247, 393, 631
BsmAI GTCTC 2 cut(s) 153, 470
BsmFI GGGAC 2 cut(s) 342, 395
BsnI GGCC 4 cut(s) 251, 257, 317, 563
Bsp1286I GDGCHC 2 cut(s) 574, 599
Bsp143I GATC 2 cut(s) 8, 552
Bsp1720I GCTNAGC 1 cut(s) 398
BspACI CCGC 3 cut(s) 241, 258, 632
BspANI GGCC 4 cut(s) 251, 257, 317, 563
BspCNI CTCAG 3 cut(s) 390, 586, 604
BspLI GGNNCC 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 624
BsrI ACTGG 1 cut(s) 219
BssAI RCCGGY 1 cut(s) 624
BssECI CCNNGG 4 cut(s) 252, 339, 387, 438
BssMI GATC 2 cut(s) 8, 552
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 2 cut(s) 254, 340
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 1 cut(s) 214
BstBAI YACGTR 1 cut(s) 513
BstC8I GCNNGC 4 cut(s) 245, 249, 634, 638
BstDEI CTNAG 3 cut(s) 398, 573, 591
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 2 cut(s) 173, 293
BstKTI GATC 2 cut(s) 11, 555
BstMAI GTCTC 2 cut(s) 153, 470
BstMBI GATC 2 cut(s) 8, 552
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 3 cut(s) 257, 290, 569
BstNI CCWGG 2 cut(s) 254, 340
BstPAI GACNNNNGTC 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 252, 338, 530
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 2 cut(s) 180, 478
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BstZI CGGCCG 1 cut(s) 561
BsuRI GGCC 4 cut(s) 251, 257, 317, 563
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 3 cut(s) 493, 599, 640
Cac8I GCNNGC 4 cut(s) 245, 249, 634, 638
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 2 cut(s) 173, 293
Cfr10I RCCGGY 1 cut(s) 624
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 346, 410
CspAI ACCGGT 1 cut(s) 624
CviAII CATG 4 cut(s) 165, 367, 427, 463
CviQI GTAC 2 cut(s) 346, 410
DdeI CTNAG 3 cut(s) 398, 573, 591
DpnI GATC 2 cut(s) 10, 554
DpnII GATC 2 cut(s) 8, 552
EaeI YGGCCR 2 cut(s) 315, 561
EagI CGGCCG 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 572
EclXI CGGCCG 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 574
Eco52I CGGCCG 1 cut(s) 561
Eco53kI GAGCTC 1 cut(s) 572
Eco57I CTGAAG 1 cut(s) 440
Eco72I CACGTG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 252, 338
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 4 cut(s) 168, 370, 430, 466
FaiI YATR 4 cut(s) 166, 368, 428, 464
FaqI GGGAC 2 cut(s) 342, 395
FatI CATG 4 cut(s) 164, 366, 426, 462
FauI CCCGC 2 cut(s) 248, 625
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 169, 258, 492
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 3 cut(s) 169, 258, 492
FspBI CTAG 1 cut(s) 600
GlaI GCGC 2 cut(s) 172, 292
GluI GCNGC 3 cut(s) 169, 258, 492
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 4 cut(s) 251, 257, 317, 563
HapII CCGG 2 cut(s) 531, 625
HhaI GCGC 2 cut(s) 173, 293
Hin1II CATG 4 cut(s) 168, 370, 430, 466
Hin6I GCGC 2 cut(s) 171, 291
HinP1I GCGC 2 cut(s) 171, 291
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 531, 625
Hpy166II GTNNAC 2 cut(s) 103, 597
Hpy188I TCNGA 1 cut(s) 158
Hpy188III TCNNGA 3 cut(s) 304, 446, 518
Hpy8I GTNNAC 2 cut(s) 103, 597
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 2 cut(s) 471, 512
HpyCH4V TGCA 7 cut(s) 4, 168, 195, 366, 597, 608, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 290, 569
HpyF3I CTNAG 3 cut(s) 398, 573, 591
HpySE526I ACGT 2 cut(s) 471, 512
Hsp92II CATG 4 cut(s) 168, 370, 430, 466
HspAI GCGC 2 cut(s) 171, 291
Kzo9I GATC 2 cut(s) 8, 552
LmnI GCTCC 2 cut(s) 350, 415
Lsp1109I GCAGC 2 cut(s) 180, 478
LweI GCATC 1 cut(s) 204
MaeI CTAG 1 cut(s) 600
MaeII ACGT 2 cut(s) 471, 512
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 10, 554
MboI GATC 2 cut(s) 8, 552
MboII GAAGA 7 cut(s) 45, 77, 80, 117, 201, 433, 547
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 574, 599
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 1 cut(s) 72
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 64, 70
MnlI CCTC 3 cut(s) 79, 215, 364
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 2 cut(s) 324, 582
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 2 cut(s) 531, 625
MspR9I CCNGG 3 cut(s) 254, 340, 532
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 2 cut(s) 254, 340
MwoI GCNNNNNNNGC 3 cut(s) 257, 290, 569
NciI CCSGG 1 cut(s) 532
NdeII GATC 2 cut(s) 8, 552
NlaIII CATG 4 cut(s) 168, 370, 430, 466
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 530
PinAI ACCGGT 1 cut(s) 624
PkrI GCNGC 3 cut(s) 170, 259, 493
PleI GAGTC 1 cut(s) 155
PmaCI CACGTG 1 cut(s) 513
PmlI CACGTG 1 cut(s) 513
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 482
Psp124BI GAGCTC 1 cut(s) 574
Psp6I CCWGG 2 cut(s) 252, 338
PspCI CACGTG 1 cut(s) 513
PspGI CCWGG 2 cut(s) 252, 338
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 250
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 2 cut(s) 347, 411
RsaNI GTAC 2 cut(s) 346, 410
SacI GAGCTC 1 cut(s) 574
SaqAI TTAA 2 cut(s) 324, 582
SatI GCNGC 3 cut(s) 169, 258, 492
Sau3AI GATC 2 cut(s) 8, 552
Sau96I GGNCC 1 cut(s) 250
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 3 cut(s) 254, 340, 532
SduI GDGCHC 2 cut(s) 574, 599
SetI ASST 8 cut(s) 19, 182, 249, 420, 474, 515, 574, 579
SfaNI GCATC 1 cut(s) 204
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 1 cut(s) 72
SsiI CCGC 3 cut(s) 241, 258, 632
SspMI CTAG 1 cut(s) 600
SstI GAGCTC 1 cut(s) 574
StyD4I CCNGG 3 cut(s) 252, 338, 530
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 2 cut(s) 474, 515
TasI AATT 1 cut(s) 72
TauI GCSGC 1 cut(s) 260
Tru1I TTAA 2 cut(s) 324, 582
Tru9I TTAA 2 cut(s) 324, 582
TscAI CASTG 2 cut(s) 493, 599
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 2 cut(s) 168, 491
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 117, 142
TspGWI ACGGA 4 cut(s) 256, 363, 397, 561
TspRI CASTG 2 cut(s) 493, 599
VneI GTGCAC 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.