pycom06g05600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004592
Physical Location & Seq
Forward (+)
8398531 .. 8398782
252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g05600.1

Sequence Viewer

Length: 252 bp
ATGTTTGGCACGGTCGACAGGGCCGCAGTTGCCAGACTCTCAAGCTCGACAGATTTCATCAAAGCATCGTGCAGAACCACTCAGTACCCTACACTCTGTCTCCAAAGCCTTGCAGTATACACAAGTGCAATAAGGCAAAGTGAAAGGCAACTAGCTCAAACTGCCTTGACAGTGAGCCTAGCCCGGCTGCGGTCTGCCGCGTCCTTCGTGGCGAAGCTAACTAGGGTTAGGCAGATTAAGCCTAGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

84

Amino Acids

9.16

Weight (kDa)

11.18

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 18 - 75 1.2e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 15, 117
AccII CGCG 1 cut(s) 200
AciI CCGC 3 cut(s) 24, 190, 198
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 189
AluBI AGCT 3 cut(s) 45, 155, 217
AluI AGCT 3 cut(s) 45, 155, 217
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 187
AspA2I CCTAGG 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 1 cut(s) 184
AvrII CCTAGG 1 cut(s) 242
BbvI GCAGC 1 cut(s) 174
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 4 cut(s) 152, 179, 222, 243
BisI GCNGC 3 cut(s) 24, 188, 198
BlnI CCTAGG 1 cut(s) 242
BlsI GCNGC 3 cut(s) 25, 189, 199
Bme1390I CCNGG 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 21
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 25
BpuMI CCSGG 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 1 cut(s) 23
BspACI CCGC 3 cut(s) 24, 190, 198
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 94
BspFNI CGCG 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 242
BssNAI GTATAC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 242
Bst1107I GTATAC 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 13, 172
BstDEI CTNAG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 200
BstMAI GTCTC 1 cut(s) 104
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 3 cut(s) 29, 161, 238
BstSCI CCNGG 1 cut(s) 182
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 174
BstZ17I GTATAC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 1 cut(s) 189
Csp6I GTAC 1 cut(s) 85
CviJI RGCY 9 cut(s) 23, 45, 108, 155, 177, 182, 187, 217, 241
CviKI_1 RGCY 9 cut(s) 23, 45, 108, 155, 177, 182, 187, 217, 241
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 242
FaiI YATR 1 cut(s) 118
FblI GTMKAC 2 cut(s) 15, 117
Fnu4HI GCNGC 3 cut(s) 24, 188, 198
Fsp4HI GCNGC 3 cut(s) 24, 188, 198
FspBI CTAG 4 cut(s) 152, 179, 222, 243
GluI GCNGC 3 cut(s) 24, 188, 198
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 184
HgaI GACGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 1 cut(s) 36
HpaII CCGG 1 cut(s) 184
Hpy166II GTNNAC 2 cut(s) 16, 118
Hpy8I GTNNAC 2 cut(s) 16, 118
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 2 cut(s) 13, 172
HpyCH4V TGCA 3 cut(s) 72, 113, 128
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 161, 238
HpyF3I CTNAG 1 cut(s) 81
LpnPI CCDG 3 cut(s) 4, 46, 197
Lsp1109I GCAGC 1 cut(s) 174
LweI GCATC 1 cut(s) 74
MaeI CTAG 4 cut(s) 152, 179, 222, 243
MlyI GAGTC 1 cut(s) 30
MseI TTAA 1 cut(s) 237
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 29, 161, 238
NciI CCSGG 1 cut(s) 184
PkrI GCNGC 3 cut(s) 25, 189, 199
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 21
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 3 cut(s) 24, 188, 198
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 184
SetI ASST 3 cut(s) 47, 157, 219
SfaNI GCATC 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
SsiI CCGC 3 cut(s) 24, 190, 198
SspMI CTAG 4 cut(s) 152, 179, 222, 243
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 2 cut(s) 13, 172
TaqI TCGA 2 cut(s) 15, 47
TauI GCSGC 2 cut(s) 26, 200
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 177
TseI GCWGC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 46
TspRI CASTG 1 cut(s) 177
XmaJI CCTAGG 1 cut(s) 242
XmiI GTMKAC 2 cut(s) 15, 117
XspI CTAG 4 cut(s) 152, 179, 222, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.