MD04G1081200.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004592
Physical Location & Seq
Forward (+)
11210463 .. 11211080
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1081200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCAAGACTCGGAGATATATTCCTTTCCTTTCTAGTCCTTTTCTTCAATGTTTGTTCCATTGGCACGGCATACTCTGCCGGCGCCGCCATTGCCAGACACTCAAGCTCCACAGATTTCATCAAAGCATCATGCAGAACCACCCAGTACCCTGCCCTCTGTGTCCAAAGCCTTGCAGCCTACGCAAGTGCAATAAGGCAAAGTGAAAGGCAACTAGCTCAAACTGCCTTGACAGTGAGCCTAGCCCGGGTGCGGTCAGCCACGTCTTTTGTGACAAAGCTGACTAGGGTTAGGGGGATCAAGCCTAGGGAGTACAGAGCTGTAAAGGACTGCATAGAAAACATGGGGGATAGCGTCGACCGGCTCGGTCAATCGGTCCGGGAACTAGGGCATATGGGGAGAGCGTTTGGGCAGGACTTCATGTGGCACATGAGCAATGTGGAGACATGGGTTAGTGCTGCACTCACTGATGAAGGAACTTGCCTTGATGGATTTTCTGGTCGTTTTATGGATGGGAATATAAAGAATGCTATAAGAAGAAGGGTCAATAATGTTGCTCACGTCACTAGCAATGCATTAGCACTTGTTACTCGCTTTGCTGCAAAACACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

206

Amino Acids

22.52

Weight (kDa)

9.97

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 39 - 195 1.7e-41 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 357
AciI CCGC 2 cut(s) 87, 253
AclWI GGATC 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 2 cut(s) 149, 314
AfiI CCNNNNNNNGG 2 cut(s) 247, 252
AgsI TTSAA 1 cut(s) 49
AjiI CACGTC 2 cut(s) 264, 562
AluBI AGCT 4 cut(s) 108, 218, 280, 320
AluI AGCT 4 cut(s) 108, 218, 280, 320
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 246
ApeKI GCWGC 3 cut(s) 176, 458, 599
AspA2I CCTAGG 1 cut(s) 305
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 3 cut(s) 247, 248, 380
AvaI CYCGRG 1 cut(s) 246
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 305
BanI GGYRCC 1 cut(s) 83
BbvI GCAGC 3 cut(s) 188, 445, 586
BccI CCATC 2 cut(s) 482, 506
BceAI ACGGC 1 cut(s) 84
BcnI CCSGG 3 cut(s) 247, 248, 380
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 7 cut(s) 35, 215, 242, 285, 306, 386, 567
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 4 cut(s) 87, 177, 459, 600
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 4 cut(s) 88, 178, 460, 601
Bme1390I CCNGG 3 cut(s) 247, 248, 380
Bme18I GGWCC 1 cut(s) 376
BmeT110I CYCGRG 1 cut(s) 246
BmgBI CACGTC 2 cut(s) 264, 562
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 3 cut(s) 247, 248, 380
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 3 cut(s) 247, 248, 380
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 246, 305
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 252
Bse118I RCCGGY 2 cut(s) 80, 360
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 3 cut(s) 90, 442, 577
BseDI CCNNGG 2 cut(s) 246, 305
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 2 cut(s) 247, 252
BseMI GCAATG 3 cut(s) 90, 442, 577
BseNI ACTGG 1 cut(s) 145
BseXI GCAGC 3 cut(s) 188, 445, 586
BsgI GTGCAG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 361
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 4 cut(s) 81, 247, 361, 379
BslI CCNNNNNNNGG 2 cut(s) 247, 252
BsmAI GTCTC 1 cut(s) 437
BsmI GAATGC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 246
Bsp143I GATC 1 cut(s) 297
BspACI CCGC 2 cut(s) 87, 253
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 3 cut(s) 90, 442, 577
BsrFI RCCGGY 2 cut(s) 80, 360
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 2 cut(s) 80, 360
BssECI CCNNGG 2 cut(s) 246, 305
BssMI GATC 1 cut(s) 297
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 82
BstF5I GGATG 1 cut(s) 517
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 300
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 1 cut(s) 297
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 5 cut(s) 77, 86, 92, 182, 224
BstSCI CCNGG 3 cut(s) 245, 246, 378
BstV1I GCAGC 3 cut(s) 188, 445, 586
BtrI CACGTC 2 cut(s) 264, 562
BtsCI GGATG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 240, 465
Cac8I GCNNGC 1 cut(s) 82
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 2 cut(s) 80, 360
Cfr13I GGNCC 1 cut(s) 376
Cfr9I CCCGGG 1 cut(s) 246
CpoI CGGWCCG 1 cut(s) 376
CseI GACGC 1 cut(s) 343
Csp6I GTAC 2 cut(s) 148, 313
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CspI CGGWCCG 1 cut(s) 376
CviAII CATG 5 cut(s) 132, 343, 421, 430, 447
CviQI GTAC 2 cut(s) 148, 313
DinI GGCGCC 1 cut(s) 85
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 376
Eco88I CYCGRG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 305
EcoT22I ATGCAT 1 cut(s) 577
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 5 cut(s) 135, 346, 424, 433, 450
FatI CATG 5 cut(s) 131, 342, 420, 429, 446
FauNDI CATATG 1 cut(s) 393
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 4 cut(s) 87, 177, 459, 600
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 4 cut(s) 87, 177, 459, 600
FspBI CTAG 7 cut(s) 35, 215, 242, 285, 306, 386, 567
GlaI GCGC 1 cut(s) 85
GluI GCNGC 4 cut(s) 87, 177, 459, 600
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 4 cut(s) 81, 247, 361, 379
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 5 cut(s) 135, 346, 424, 433, 450
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 9
HpaII CCGG 4 cut(s) 81, 247, 361, 379
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 14
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 2 cut(s) 467, 534
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 2 cut(s) 263, 561
HpyCH4V TGCA 7 cut(s) 135, 176, 191, 333, 461, 575, 602
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 86, 92, 182, 224
HpySE526I ACGT 2 cut(s) 263, 561
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 5 cut(s) 135, 346, 424, 433, 450
HspAI GCGC 1 cut(s) 84
KasI GGCGCC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Kzo9I GATC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 9 cut(s) 94, 109, 158, 165, 260, 374, 392, 398, 483
Lsp1109I GCAGC 3 cut(s) 188, 445, 586
LweI GCATC 1 cut(s) 137
MaeI CTAG 7 cut(s) 35, 215, 242, 285, 306, 386, 567
MaeII ACGT 2 cut(s) 263, 561
MaeIII GTNAC 3 cut(s) 271, 562, 586
MalI GATC 1 cut(s) 299
MboI GATC 1 cut(s) 297
MboII GAAGA 2 cut(s) 37, 549
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 3
MnlI CCTC 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 577
MroNI GCCGGC 1 cut(s) 80
MspI CCGG 4 cut(s) 81, 247, 361, 379
MspR9I CCNGG 3 cut(s) 247, 248, 380
Mva1269I GAATGC 1 cut(s) 532
MwoI GCNNNNNNNGC 5 cut(s) 77, 86, 92, 182, 224
NaeI GCCGGC 1 cut(s) 82
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 3 cut(s) 247, 248, 380
NdeI CATATG 1 cut(s) 393
NdeII GATC 1 cut(s) 297
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 5 cut(s) 135, 346, 424, 433, 450
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 271, 562
NsiI ATGCAT 1 cut(s) 577
PctI GAATGC 1 cut(s) 532
PdiI GCCGGC 1 cut(s) 82
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 4 cut(s) 88, 178, 460, 601
PleI GAGTC 1 cut(s) 3
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 3
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 376
RsaI GTAC 2 cut(s) 149, 314
RsaNI GTAC 2 cut(s) 148, 313
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SalI GTCGAC 1 cut(s) 356
SatI GCNGC 4 cut(s) 87, 177, 459, 600
Sau3AI GATC 1 cut(s) 297
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 3
ScrFI CCNGG 3 cut(s) 247, 248, 380
SetI ASST 6 cut(s) 110, 220, 266, 282, 322, 564
SfaNI GCATC 1 cut(s) 137
SfoI GGCGCC 1 cut(s) 85
SinI GGWCC 1 cut(s) 376
SmaI CCCGGG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
SsiI CCGC 2 cut(s) 87, 253
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 7 cut(s) 35, 215, 242, 285, 306, 386, 567
StyD4I CCNGG 3 cut(s) 245, 246, 378
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 2 cut(s) 266, 564
TaqI TCGA 1 cut(s) 357
TaqII GACCGA 2 cut(s) 356, 364
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 89
TscAI CASTG 2 cut(s) 240, 472
TseFI GTSAC 2 cut(s) 271, 562
TseI GCWGC 3 cut(s) 176, 458, 599
Tsp45I GTSAC 2 cut(s) 271, 562
TspDTI ATGAA 3 cut(s) 109, 409, 486
TspMI CCCGGG 1 cut(s) 246
TspRI CASTG 2 cut(s) 240, 472
VpaK11BI GGWCC 1 cut(s) 376
XmaI CCCGGG 1 cut(s) 246
XmaJI CCTAGG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 7 cut(s) 35, 215, 242, 285, 306, 386, 567
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.