MD00G1105000.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004592
Physical Location & Seq
Forward (+)
21987835 .. 21988419
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1105000.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGCTAGAGCCTCAGTTTTCTTGCTTCTTCTTCCCATCTTCTACCTTGTTGGCACAGCCAAATCTGCATCCACCACAACTACTACAAACTTCATCGAAACCTCATGCAGGGCAACCACCTACCCACAAGTCTGCATCCAATCCCTCTCTTCCTACGCCAACTCTATCCAGCAAAGCCCCCGCCAGCTGGCCCAGGCCGCCTTGTCCGTCAGCCTAACGAAAGCAAAGAGCGCCAAAACATTCGTGACCAAGTTGGCCAAGTTTAAGGGACTAAAATCCAGAGAGTACGGAGCCATCAAGGACTGCTTGGAGGAGATGGGGGACAGCGTGGACAGGATGAGCAAGTCGGTGACGGAACTGAAGAACATGGGGAAATCCAAGGGTCAGGATTTCTTGTGGCACATGAGCAATGTGGAGACTTGGGTCAGTGCCGCTTTGACCGATGATAATACGTGTCTTGATGGGTTCTCCGGGAAGGCCTTGAACGGCAAGATCAAATCCTCCGTTAGAGCTCAGGTGCTTAATGTTGCTCAGTGCACTAGTAACGCACTCGCCTTGTGCAACCGGTTTGCTGGCAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

195

Amino Acids

21.0

Weight (kDa)

9.46

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 186 5.3e-42 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 181, 198, 432
AcoI YGGCCR 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 380
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 108, 187
AflIII ACRYGT 1 cut(s) 452
AgeI ACCGGT 1 cut(s) 564
AgsI TTSAA 1 cut(s) 484
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 2 cut(s) 187, 512
AluI AGCT 2 cut(s) 187, 512
Alw21I GWGCWC 2 cut(s) 514, 539
Alw26I GTCTC 1 cut(s) 410
Alw44I GTGCAC 1 cut(s) 535
AoxI GGCC 4 cut(s) 189, 195, 255, 477
ApaLI GTGCAC 1 cut(s) 535
AsiGI ACCGGT 1 cut(s) 564
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 539
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 1 cut(s) 514
Bbv12I GWGCWC 2 cut(s) 514, 539
BccI CCATC 4 cut(s) 44, 302, 310, 455
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 1 cut(s) 194
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 410
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 2 cut(s) 6, 540
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 2 cut(s) 198, 432
BlsI GCNGC 2 cut(s) 199, 433
Bme1390I CCNGG 2 cut(s) 194, 472
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 2 cut(s) 194, 472
BmsI GCATC 2 cut(s) 77, 144
BoxI GACNNNNGTC 1 cut(s) 422
Bpu10I CCTNAGC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 192, 378
BsaWI WCCGGW 1 cut(s) 564
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 187
Bse118I RCCGGY 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 192, 378
BseGI GGATG 3 cut(s) 68, 135, 342
BseLI CCNNNNNNNGG 2 cut(s) 108, 187
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 3 cut(s) 27, 527, 545
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 539
BshFI GGCC 4 cut(s) 191, 197, 257, 479
BshTI ACCGGT 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 514, 539
BsiSI CCGG 2 cut(s) 471, 565
BslFI GGGAC 2 cut(s) 282, 335
BslI CCNNNNNNNGG 2 cut(s) 108, 187
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 2 cut(s) 282, 335
BsnI GGCC 4 cut(s) 191, 197, 257, 479
Bsp1286I GDGCHC 2 cut(s) 514, 539
Bsp143I GATC 1 cut(s) 492
BspACI CCGC 3 cut(s) 181, 198, 432
BspANI GGCC 4 cut(s) 191, 197, 257, 479
BspCNI CTCAG 3 cut(s) 26, 526, 544
BspLI GGNNCC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 564
BssECI CCNNGG 2 cut(s) 192, 378
BssMI GATC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 453
BstC8I GCNNGC 3 cut(s) 185, 189, 574
BstDEI CTNAG 3 cut(s) 13, 513, 531
BstENI CCTNNNNNAGG 1 cut(s) 106
BstF5I GGATG 3 cut(s) 68, 135, 342
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 3 cut(s) 65, 197, 230
BstNI CCWGG 1 cut(s) 194
BstPAI GACNNNNGTC 1 cut(s) 422
BstSCI CCNGG 2 cut(s) 192, 470
BstSLI GKGCMC 1 cut(s) 539
BsuRI GGCC 4 cut(s) 191, 197, 257, 479
BtsCI GGATG 3 cut(s) 68, 135, 342
BtsIMutI CAGTG 3 cut(s) 433, 539, 580
Cac8I GCNNGC 3 cut(s) 185, 189, 574
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 564
Cfr13I GGNCC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 286
CspAI ACCGGT 1 cut(s) 564
CviAII CATG 3 cut(s) 105, 367, 403
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 3 cut(s) 13, 513, 531
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 378
Eco147I AGGCCT 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 514
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 3 cut(s) 108, 370, 406
FaiI YATR 3 cut(s) 106, 368, 404
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 2 cut(s) 282, 335
FatI CATG 3 cut(s) 104, 366, 402
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 198, 432
FokI GGATG 3 cut(s) 55, 122, 349
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 2 cut(s) 198, 432
FspBI CTAG 2 cut(s) 6, 540
GlaI GCGC 1 cut(s) 232
GluI GCNGC 2 cut(s) 198, 432
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 4 cut(s) 191, 197, 257, 479
HapII CCGG 2 cut(s) 471, 565
HhaI GCGC 1 cut(s) 233
Hin1II CATG 3 cut(s) 108, 370, 406
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HpaII CCGG 2 cut(s) 471, 565
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 331, 537
Hpy188III TCNNGA 4 cut(s) 244, 279, 386, 458
Hpy8I GTNNAC 2 cut(s) 331, 537
HpyAV CCTTC 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 5 cut(s) 68, 108, 135, 537, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 197, 230
HpyF3I CTNAG 3 cut(s) 13, 513, 531
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 3 cut(s) 108, 370, 406
HspAI GCGC 1 cut(s) 231
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 290
LweI GCATC 2 cut(s) 77, 144
MaeI CTAG 2 cut(s) 6, 540
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 244, 349, 542
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 5 cut(s) 20, 23, 31, 141, 373
MhlI GDGCHC 2 cut(s) 514, 539
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MnlI CCTC 5 cut(s) 22, 112, 155, 304, 511
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 2 cut(s) 264, 522
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 2 cut(s) 471, 565
MspR9I CCNGG 2 cut(s) 194, 472
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 3 cut(s) 65, 197, 230
NciI CCSGG 1 cut(s) 472
NdeII GATC 1 cut(s) 492
NlaIII CATG 3 cut(s) 108, 370, 406
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 2 cut(s) 244, 349
PceI AGGCCT 1 cut(s) 479
PfoI TCCNGGA 1 cut(s) 470
PinAI ACCGGT 1 cut(s) 564
PkrI GCNGC 2 cut(s) 199, 433
Ppu21I YACGTR 1 cut(s) 453
PshAI GACNNNNGTC 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 1 cut(s) 514
SaqAI TTAA 2 cut(s) 264, 522
SatI GCNGC 2 cut(s) 198, 432
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 194, 472
SduI GDGCHC 2 cut(s) 514, 539
SetI ASST 7 cut(s) 48, 104, 122, 189, 455, 514, 519
SfaNI GCATC 2 cut(s) 77, 144
SpeI ACTAGT 1 cut(s) 539
SseBI AGGCCT 1 cut(s) 479
SsiI CCGC 3 cut(s) 181, 198, 432
SspMI CTAG 2 cut(s) 6, 540
SstI GAGCTC 1 cut(s) 514
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 192, 470
StyI CCWWGG 1 cut(s) 378
TaiI ACGT 1 cut(s) 455
TaqI TCGA 1 cut(s) 96
TaqII GACCGA 1 cut(s) 455
TauI GCSGC 2 cut(s) 200, 434
Tru1I TTAA 2 cut(s) 264, 522
Tru9I TTAA 2 cut(s) 264, 522
TscAI CASTG 2 cut(s) 433, 539
TseFI GTSAC 2 cut(s) 244, 349
Tsp45I GTSAC 2 cut(s) 244, 349
TspDTI ATGAA 1 cut(s) 82
TspGWI ACGGA 4 cut(s) 196, 303, 368, 493
TspRI CASTG 2 cut(s) 433, 539
VneI GTGCAC 1 cut(s) 535
XagI CCTNNNNNAGG 1 cut(s) 106
XspI CTAG 2 cut(s) 6, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.