Rh1AG396200

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004592
Physical Location & Seq
Reverse (-)
62394569 .. 62395520
952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG396200.1

Sequence Viewer

Length: 582 bp
ATGGCTAGATCCTCCATTCTCTTTCTACTTCTTGCCCTCTTCGCCCTCACCGCCAACTCTGCTTCCACCACCACTGCCTCCAACTTCGTCAAAACCTCATGCAGCACCACCACCTACCCTGACCTCTGCGTTCAGTCCCTCTCATCATTCTCCACCGCGATCCAGCAAAGCCCTCGCCAGCTGGCGCAGACCGCCCTCTCCGTCAGCCTCACCCGAGCCCAGTTCGCCAAGTCCTTCGTCACCAAGCTGACCAAGTTCAAAGGTTTGAGCCACAAGGAGTACGAGGCCATCGACGACTGCCTTGAGGAGATGGGTGACAGCGTCGACCGGCTGAGCAAGTCCGTGGACGAGCTGAAGAAGATGGGGAAGTCATCCAAGGGCCAGGATTTCTTATGGCACATGAGCAATGTCGAGACTTGGGTCAGCGCTGCGTTGACAGACGACAACACTTGCCTTGATGGGTTCTCCGGGAAGGCCATGAATGGCAAGATCAAGGCCTCCATTAGAGCTCAGGTGCTTAATGTTGCTAAGGTTACTAGTAATGCTCTGGCCCTTTGCAACCACTTTGCCTCCAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

193

Amino Acids

20.93

Weight (kDa)

8.97

Isoelectric Point (pI)

26.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 185 4.4e-42 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 158
AciI CCGC 3 cut(s) 51, 156, 192
AclWI GGATC 2 cut(s) 3, 154
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 1 cut(s) 281
AfeI AGCGCT 1 cut(s) 427
AgsI TTSAA 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 4 cut(s) 181, 247, 352, 509
AluI AGCT 4 cut(s) 181, 247, 352, 509
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 2 cut(s) 3, 154
Ama87I CYCGRG 1 cut(s) 213
Aor51HI AGCGCT 1 cut(s) 427
AoxI GGCC 5 cut(s) 285, 379, 474, 495, 549
ApeKI GCWGC 2 cut(s) 102, 428
AspLEI GCGC 2 cut(s) 187, 428
AspS9I GGNCC 2 cut(s) 379, 550
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 4 cut(s) 40, 202, 232, 326
AvaI CYCGRG 1 cut(s) 213
BanII GRGCYC 2 cut(s) 220, 511
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 114, 415
BccI CCATC 4 cut(s) 296, 304, 355, 452
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 383
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 407
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 2 cut(s) 6, 537
BfoI RGCGCY 1 cut(s) 429
BisI GCNGC 2 cut(s) 103, 429
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 104, 430
Bme1390I CCNGG 2 cut(s) 383, 469
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 379, 550
BmrFI CCNGG 2 cut(s) 383, 469
BmrI ACTGGG 1 cut(s) 214
BmuI ACTGGG 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 419
Bpu10I CCTNAGC 2 cut(s) 510, 528
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 1 cut(s) 469
BsaJI CCNNGG 2 cut(s) 342, 375
BsaXI ACNNNNNCTCC 1 cut(s) 554
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 342, 375
BseGI GGATG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 323, 524
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 114, 415
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 5 cut(s) 287, 381, 476, 497, 551
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 511
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 328, 468
BslFI GGGAC 1 cut(s) 121
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 5 cut(s) 287, 381, 476, 497, 551
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 220, 511
Bsp143I GATC 3 cut(s) 8, 159, 489
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 3 cut(s) 51, 156, 192
BspANI GGCC 5 cut(s) 287, 381, 476, 497, 551
BspCNI CTCAG 2 cut(s) 324, 523
BspFNI CGCG 1 cut(s) 158
BspPI GGATC 2 cut(s) 3, 154
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 342, 375
BssMI GATC 3 cut(s) 8, 159, 489
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 383
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 179, 183
BstDEI CTNAG 3 cut(s) 332, 510, 528
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 2 cut(s) 187, 428
BstKTI GATC 3 cut(s) 11, 162, 492
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 3 cut(s) 8, 159, 489
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
BstNI CCWGG 1 cut(s) 383
BstPAI GACNNNNGTC 1 cut(s) 419
BstSCI CCNGG 2 cut(s) 381, 467
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 2 cut(s) 114, 415
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 5 cut(s) 287, 381, 476, 497, 551
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 179, 183
CfoI GCGC 2 cut(s) 187, 428
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 379, 550
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 280
CviAII CATG 3 cut(s) 99, 400, 478
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 3 cut(s) 332, 510, 528
DpnI GATC 3 cut(s) 10, 161, 491
DpnII GATC 3 cut(s) 8, 159, 489
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 1 cut(s) 497
Eco24I GRGCYC 2 cut(s) 220, 511
Eco47III AGCGCT 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 2 cut(s) 220, 511
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 102, 403, 481
FaiI YATR 4 cut(s) 100, 394, 401, 479
FaqI GGGAC 1 cut(s) 121
FatI CATG 3 cut(s) 98, 399, 477
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 103, 429
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 220, 511
Fsp4HI GCNGC 2 cut(s) 103, 429
FspBI CTAG 2 cut(s) 6, 537
GlaI GCGC 2 cut(s) 186, 427
GluI GCNGC 2 cut(s) 103, 429
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 5 cut(s) 287, 381, 476, 497, 551
HapII CCGG 2 cut(s) 328, 468
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 187, 428
Hin1II CATG 3 cut(s) 102, 403, 481
Hin6I GCGC 2 cut(s) 185, 426
HinP1I GCGC 2 cut(s) 185, 426
HincII GTYRAC 2 cut(s) 325, 435
HindII GTYRAC 2 cut(s) 325, 435
HpaII CCGG 2 cut(s) 328, 468
HphI GGTGA 4 cut(s) 40, 202, 232, 326
Hpy166II GTNNAC 3 cut(s) 325, 346, 435
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 3 cut(s) 325, 346, 435
Hpy99I CGWCG 2 cut(s) 296, 326
HpyAV CCTTC 2 cut(s) 244, 466
HpyCH4V TGCA 2 cut(s) 102, 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
HpyF3I CTNAG 3 cut(s) 332, 510, 528
Hsp92II CATG 3 cut(s) 102, 403, 481
HspAI GCGC 2 cut(s) 185, 426
Kzo9I GATC 3 cut(s) 8, 159, 489
Lsp1109I GCAGC 2 cut(s) 114, 415
MaeI CTAG 2 cut(s) 6, 537
MaeIII GTNAC 3 cut(s) 238, 314, 532
MalI GATC 3 cut(s) 10, 161, 491
MboI GATC 3 cut(s) 8, 159, 489
MboII GAAGA 3 cut(s) 31, 367, 370
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 220, 511
MmeI TCCRAC 1 cut(s) 105
MseI TTAA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 2 cut(s) 328, 468
MspR9I CCNGG 2 cut(s) 383, 469
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
NciI CCSGG 1 cut(s) 469
NdeII GATC 3 cut(s) 8, 159, 489
NlaIII CATG 3 cut(s) 102, 403, 481
NmuCI GTSAC 2 cut(s) 238, 314
PceI AGGCCT 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 467
PkrI GCNGC 2 cut(s) 104, 430
PshAI GACNNNNGTC 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspPI GGNCC 2 cut(s) 379, 550
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 1 cut(s) 511
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 2 cut(s) 103, 429
Sau3AI GATC 3 cut(s) 8, 159, 489
Sau96I GGNCC 2 cut(s) 379, 550
ScrFI CCNGG 2 cut(s) 383, 469
SduI GDGCHC 2 cut(s) 220, 511
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 3 cut(s) 51, 156, 192
SspMI CTAG 2 cut(s) 6, 537
SstI GAGCTC 1 cut(s) 511
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 381, 467
StyI CCWWGG 1 cut(s) 375
TaqI TCGA 3 cut(s) 291, 324, 411
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 238, 314
TseI GCWGC 2 cut(s) 102, 428
Tsp45I GTSAC 2 cut(s) 238, 314
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 2 cut(s) 190, 331
TspRI CASTG 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 2 cut(s) 6, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.