FvH4_7g27850

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004592
Physical Location & Seq
Reverse (-)
20731674 .. 20732255
582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27850.t1

Sequence Viewer

Length: 582 bp
ATGGCTAGATCCTCCATTCTCTTCCTCCTTCTTGCCCTCTTCGCCCTCACAGCCAACTCTGCCTCCACCACCACTGCCTCCAACTTCATCAAAACCTCATGCAGCGCCACCACGTACCCAGACCTCTGCGTTCAGTCCCTCTCTTCATACTCCAACGCCATCCAGCAAAGCCCTCGCCAGCTAGCCCAGACCGCCCTGTCCGTCAGCCTAACCAGAGCCCAGTACGCCAAGTCATTCGTCACCAAGCTGACCAAGTTCAAGGGCTTGAGCCACAAGGAGTACGAGGCCATCGACGACTGCCTTGAGGAGATGGGCGACAGCGTCGACCGGCTGAGCAAATCTGTGGACGAGCTGAAGAAGATGGGCAAGTCATCCAAGGGTCAGGATTTCATATGGCACATGAGCAATGTCGAGACTTGGGTTAGTGCTGCTTTGACTGACGACAACACTTGCCTTGATGGGTTCTCCGGGAAGGCCATGGATGGCAAGATCAAGGCCTCCATTAGAGCTCAGGTGCTTAACGTTGCCAAGGTTACTAGTAATGCTCTGGCTCTTTGCAACCACTTTGCCTCCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

194

Amino Acids

20.95

Weight (kDa)

8.56

Isoelectric Point (pI)

27.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 28 - 185 5.4e-43 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 324
AciI CCGC 1 cut(s) 192
AclI AACGTT 1 cut(s) 522
AclWI GGATC 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 3 cut(s) 116, 224, 281
AgsI TTSAA 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 536
AluBI AGCT 4 cut(s) 181, 247, 352, 509
AluI AGCT 4 cut(s) 181, 247, 352, 509
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 1 cut(s) 3
AoxI GGCC 3 cut(s) 285, 474, 495
ApeKI GCWGC 2 cut(s) 102, 428
AspLEI GCGC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 232
AsuNHI GCTAGC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 220, 511
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 114, 415
BccI CCATC 6 cut(s) 167, 296, 304, 355, 452, 476
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 407
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 3 cut(s) 6, 182, 537
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 2 cut(s) 103, 429
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 104, 430
Bme1390I CCNGG 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 469
BmrI ACTGGG 1 cut(s) 214
BmtI GCTAGC 1 cut(s) 185
BmuI ACTGGG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 286, 323
BpuMI CCSGG 1 cut(s) 469
BsaAI YACGTR 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 375, 477, 528
BsaXI ACNNNNNCTCC 3 cut(s) 47, 77, 554
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 375, 477, 528
BseGI GGATG 3 cut(s) 159, 371, 487
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 323, 524
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 114, 415
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 287, 476, 497
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 2 cut(s) 328, 468
BslFI GGGAC 1 cut(s) 121
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 3 cut(s) 287, 476, 497
Bsp1286I GDGCHC 2 cut(s) 220, 511
Bsp143I GATC 2 cut(s) 8, 489
Bsp1720I GCTNAGC 1 cut(s) 332
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 1 cut(s) 192
BspANI GGCC 3 cut(s) 287, 476, 497
BspCNI CTCAG 2 cut(s) 324, 523
BspOI GCTAGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 3
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 3 cut(s) 375, 477, 528
BssMI GATC 2 cut(s) 8, 489
BssT1I CCWWGG 3 cut(s) 375, 477, 528
Bst6I CTCTTC 3 cut(s) 26, 44, 148
BstBAI YACGTR 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 179, 183
BstDEI CTNAG 2 cut(s) 332, 510
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 3 cut(s) 159, 371, 487
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 11, 492
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 2 cut(s) 8, 489
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
BstSCI CCNGG 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 114, 415
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 3 cut(s) 287, 476, 497
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 3 cut(s) 159, 371, 487
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 179, 183
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 327
CseI GACGC 1 cut(s) 310
Csp6I GTAC 3 cut(s) 115, 223, 280
CviAII CATG 3 cut(s) 99, 400, 478
CviQI GTAC 3 cut(s) 115, 223, 280
DdeI CTNAG 2 cut(s) 332, 510
DpnI GATC 2 cut(s) 10, 491
DpnII GATC 2 cut(s) 8, 489
DrdI GACNNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 196
Eam1104I CTCTTC 3 cut(s) 26, 44, 148
EarI CTCTTC 3 cut(s) 26, 44, 148
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 3 cut(s) 375, 477, 528
Eco147I AGGCCT 1 cut(s) 497
Eco24I GRGCYC 2 cut(s) 220, 511
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 509
EcoT14I CCWWGG 3 cut(s) 375, 477, 528
EcoT38I GRGCYC 2 cut(s) 220, 511
ErhI CCWWGG 3 cut(s) 375, 477, 528
FaeI CATG 3 cut(s) 102, 403, 481
FaiI YATR 6 cut(s) 100, 148, 392, 394, 401, 479
FaqI GGGAC 1 cut(s) 121
FatI CATG 3 cut(s) 98, 399, 477
FauNDI CATATG 1 cut(s) 392
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 103, 429
FokI GGATG 3 cut(s) 146, 358, 494
FriOI GRGCYC 2 cut(s) 220, 511
Fsp4HI GCNGC 2 cut(s) 103, 429
FspBI CTAG 3 cut(s) 6, 182, 537
GlaI GCGC 1 cut(s) 106
GluI GCNGC 2 cut(s) 103, 429
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 3 cut(s) 287, 476, 497
HapII CCGG 2 cut(s) 328, 468
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 102, 403, 481
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HpaII CCGG 2 cut(s) 328, 468
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 325, 346
Hpy188III TCNNGA 2 cut(s) 383, 412
Hpy8I GTNNAC 2 cut(s) 325, 346
Hpy99I CGWCG 2 cut(s) 296, 326
HpyAV CCTTC 2 cut(s) 38, 466
HpyCH4IV ACGT 2 cut(s) 113, 522
HpyCH4V TGCA 2 cut(s) 102, 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
HpyF3I CTNAG 2 cut(s) 332, 510
HpySE526I ACGT 2 cut(s) 113, 522
Hsp92II CATG 3 cut(s) 102, 403, 481
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 8, 489
Lsp1109I GCAGC 2 cut(s) 114, 415
MaeI CTAG 3 cut(s) 6, 182, 537
MaeII ACGT 2 cut(s) 113, 522
MaeIII GTNAC 2 cut(s) 238, 532
MalI GATC 2 cut(s) 10, 491
MboI GATC 2 cut(s) 8, 489
MboII GAAGA 5 cut(s) 13, 31, 135, 367, 370
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 220, 511
MmeI TCCRAC 2 cut(s) 105, 177
MseI TTAA 1 cut(s) 519
MspI CCGG 2 cut(s) 328, 468
MspR9I CCNGG 1 cut(s) 469
MwoI GCNNNNNNNGC 5 cut(s) 41, 50, 59, 191, 224
NciI CCSGG 1 cut(s) 469
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 1 cut(s) 392
NdeII GATC 2 cut(s) 8, 489
NheI GCTAGC 1 cut(s) 181
NlaIII CATG 3 cut(s) 102, 403, 481
NmuCI GTSAC 1 cut(s) 238
PceI AGGCCT 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 467
PkrI GCNGC 2 cut(s) 104, 430
Ppu21I YACGTR 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 511
Psp1406I AACGTT 1 cut(s) 522
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 3 cut(s) 116, 224, 281
RsaNI GTAC 3 cut(s) 115, 223, 280
SacI GAGCTC 1 cut(s) 511
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 2 cut(s) 103, 429
Sau3AI GATC 2 cut(s) 8, 489
ScrFI CCNGG 1 cut(s) 469
SduI GDGCHC 2 cut(s) 220, 511
SmlI CTYRAG 2 cut(s) 265, 302
SmoI CTYRAG 2 cut(s) 265, 302
SpeI ACTAGT 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 1 cut(s) 192
SspMI CTAG 3 cut(s) 6, 182, 537
SstI GAGCTC 1 cut(s) 511
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 1 cut(s) 467
StyI CCWWGG 3 cut(s) 375, 477, 528
TaiI ACGT 2 cut(s) 116, 525
TaqI TCGA 3 cut(s) 291, 324, 411
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 102, 428
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 76, 135, 379
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 3 cut(s) 6, 182, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.