Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0004592

Physical Location & Seq

Genomic Coordinates Forward (+)

49634125 .. 49651157

Gene Structure 17033 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0384600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGTCGAAATCGTCCTCTGCCCTCTTCGACCTTCTCCTCCAGTCATCGGAGCCGATCGCCGAATCTCTGACCAAAACGCCGTACACGCCGTCACAGAGCACCAACGTCTCCGTGAAAGCCTTACTGGAGCCTCTCCTTCCTCGAAACTCTAACCCTAACATCGACCTCCGCGCCTCAATCCGAGACTTCACTCTCGCCTGTGCTCTACTCTCCTCCTCCCAATCCTCAACACACGAGCTTCTCTCGTGGATCCCCGAACACCTCGCGGCCGCTGCTGACCGGGCGGTTCTGGAATTCTCGAAAGCTTACTCCGATTACGGCGAGAAATCTGGTGATGGTTTGGTGGTGGAGTTGATGCCTGAGGTGATGCCGTTGCTGAAGGAGAGGATCAAGGAGAGCTCGATTGATAAATCGGCGGACGGGGATGAGGTCTCGGCGGCGTCGGCGAGAGTGCCGGTTGGGTTTGCCATTGTGGCTGCTTATCAGTTTAGGTGGTTTGTTACACAGATTGATTATTCGGATTTCGGAAAGTTGTGTGCTTTGGCTGTTCCTTGCGCTTTGACGGCTCTGGATCACTGGTCGCCGGAGATTAAAGGGCAAGGCATGATAAGCTTTATACATATCGCCAAAAGTGTAGATGCTGCTGAACTAGCTTGGTGTCAAGATGCAATTCTTGATGCATGCTGCCAGAATATTGCTTCTAGTGACGAGATATGGCATCTTGTGGTGGAGATGTCAGTACTTGTGGTGACTCGTACCCAACAGAGTAATCCTCGTAGTGCATGGTTTGATAAAATTCTAAATGAAATGTTAAGTCACTTGGAGCGCCAGCCTAGGAACAAGGAGCGCCGTGTTCCATGGCTTCGTTATGTAGAGCCGCTTTTTAATGGTGTTGGTCTGGTGTTACTAGCTCATTTCAGTCGCATTTTCCCTCTTTTCTTTAAGTGGATGCATGCTGATGATGATGAGACTGTTTTACAGGTTCTCAAACAGATTGAAACAGTTATAAAGCTAACATGGATTAGGAATACACCATATGTGGAAAGGTTGGTGGATGAACTTGCTACTTTGCACAAAGAAGCAGCATTGAAAAGATCACGTGAAGAAATTAGAAATCTTGTTATTCGTATACTGATCCTGCTCCATCAATGCAAGGGTCTGCAGTTCGAAGCAGCCTGGGGCAAGCACAGGGATGATCCAAACTTGGCAACAATTGCTCCCTCTTTAAGTGAAAGAAGATCAACAATGGTTATCAACGTTGGACCCTTCTCTCTCAATATTGCAACGTCGTCGTCATCTAACCCCGAAATATGGGTGGCGGCGGCTGCAGTTCCGGTTCCTGTTCTAGAGGTTAGAACTTTGATGGTTCTGTTTTCGGTTAAATACATTGAGATTTTCCAGAGCCTCTGTAGTGGGCAGGTTTCTTTGGCAGAGGATCAAAGATTAAGCATTCATGTTTGGAATGGGAGGCGTTGGCAAGATGTGTTGATCACCTGTGTATTGGTGTCTTTACGGTTCTTTCGCAAGTGTTTTTTGTCTATTGAATATTTTGAATGCGTGTTAGAACATATTTTTCAAGCACCATACATGGATGTGCTTTCAATAAGTGGTATTGCTCTGAAGTACATCAGAACAGCCCTGAATATGGTGAAAGGAGGGGAAATGCTGGAGGACAAGGAGAGGAGAAGGAAGGTGGGAGTTGTAAATTCATATTTGGAATGGGAGGCATTGGCAAGAAGTACTGTGATTCAAGGGCAGTTTTTTCGGTACCTTGTGCTGTCCAGCATTGTTATTGATGCGCGAATAGTGGCTCGCTCAACATCGACGTCGAGGACCTCGGCACTTCCTCCTACTACTCCATCGCCGCCGCGTCTCCTCCTCGCCTTCGACACCAACTACAGCACCGGCGTTGGCGTCGTGACCTTCGCCAACAAGTTCCCCGGAGTCTTTGCGGCCACGTGTCTCTCCCTGTCCGACGCCCTCAACGCCCAGCTCCAACGTCCTCCCGTCTCCGGCATGTCCATGGCGTCGGAATCCGCCATCGAGATCGTCGACACGTGGCTCAACACTCCCTTCAAAGCGCCCTGTCCTGCATCGAAGTCGAAACCTTGGCCAGAAATTGGAGCTGATTCTTATTTGAAGGAGAGCTCTTGTTCCATCTGCTGTTTGGTGAAGAATCGGAATTTGAATCCGATTGATATAATTTCGGGTCGTTCAATGAAGATATTGAGTTCTTTATCAAGCGGGATGGGAAATGGGATATGTTCTTTGATGAAGATCTCGAGGCTGCGAACAAGGCTATTGATCAAGAAATTGGAGAATGGGGGCATTCTATCAGTACTGGATGTTTGGAATGGGAGGCGTTGGCAAGAAGTAGAAATCACTTTGTGTGTTTTGTTGCCTCGTGGTTCTTGCGCACAGGGAGGTTCAGTCGGATTTAGCTTGGTGCTCAGACGCCCACCAACGTCTCATGTGAACAAGGCATCCAAGGCTGCTTTGGAACACAATGACCATCAACCAGCCGATGAGCACTATATTGGTCGGAGCCGCATGTTTCGGGGAAGGAGTTACTCCTGCTCGACCCACTTGACCATCACTCCATCGTCACAACTCACAAGTTTCAATCGTCAGATCATGATCGAGGCGTGCTTTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PMEI

Protein Analysis

885

Amino Acids

98.57

Weight (kDa)

8.45

Isoelectric Point (pI)

53.79

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004592)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62770 AT4G12390
fragaria_vesca	FvH4_5g00430 FvH4_7g27850
malus_domestica	MD00G1105000.v1.1 MD04G1081200.v1.1 MD07G1255800.v1.1
prunus_persica	Prupe.2G279900_v2.0.a1 Prupe.5G076900_v2.0.a1
pyrus_communis	pycom06g05600
rosa_chinensis	RchiOBHm_Chr1g0374721 RchiOBHm_Chr7g0202081
rosa_laevigata	RLG00000003627 RLG00000026718
rosa_multiflora	Rmu_co8511593.1_g000001 Rmu_sc0002063.1_g000001 Rmu_sc0006592.1_g000011 Rmu_sc0010587.1_g000003
rosa_roxburghii	Rroxscaffold_4G00283280
rosa_rugosa	Rorug01G0384600 Rorug07G0067800
rosa_samantha	Rh1AG396200 Rh1BG360300 Rh1CG373700 Rh1DG391300 Rh7AG195300 Rh7BG196600 Rh7CG206400 Rh7DG201900
rosa_wichuraiana	Rw1G035130 Rw7G017100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1007
AatII	GACGTC	1 cut(s)	1829
Acc16I	TGCGCA	1 cut(s)	2416
Acc36I	ACCTGC	1 cut(s)	1408
Acc65I	GGTACC	1 cut(s)	1767
AccB1I	GGYRCC	1 cut(s)	1767
AccB7I	CCANNNNNTGG	1 cut(s)	2120
AccI	GTMKAC	2 cut(s)	1129, 2052
AccII	CGCG	4 cut(s)	171, 266, 1801, 1870
AclI	AACGTT	1 cut(s)	1257
AclWI	GGATC	7 cut(s)	244, 257, 395, 579, 1129, 1190, 1443
AcoI	YGGCCR	3 cut(s)	267, 1953, 2111
AcsI	RAATTY	4 cut(s)	293, 795, 1705, 2182
AcuI	CTGAAG	2 cut(s)	398, 1640
AcvI	CACGTG	3 cut(s)	1100, 1959, 2058
AcyI	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
AdeI	CACNNNGTG	1 cut(s)	2388
AfaI	GTAC	7 cut(s)	83, 741, 757, 1625, 1741, 1769, 2340
AfiI	CCNNNNNNNGG	5 cut(s)	46, 1311, 1645, 2012, 2120
AflIII	ACRYGT	2 cut(s)	1958, 2055
AjnI	CCWGG	1 cut(s)	1175
AloI	GAACNNNNNNTCC	2 cut(s)	2468, 2500
Alw21I	GWGCWC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
Alw26I	GTCTC	8 cut(s)	112, 177, 436, 962, 1877, 1967, 2014, 2472
AlwI	GGATC	7 cut(s)	244, 257, 395, 579, 1129, 1190, 1443
Ama87I	CYCGRG	1 cut(s)	2281
AoxI	GGCC	3 cut(s)	267, 1953, 2111
ApeKI	GCWGC	9 cut(s)	272, 476, 641, 684, 1082, 1172, 1325, 2287, 2492
ApoI	RAATTY	4 cut(s)	293, 795, 1705, 2182
Asp718I	GGTACC	1 cut(s)	1767
AspA2I	CCTAGG	1 cut(s)	833
AspLEI	GCGC	7 cut(s)	173, 557, 828, 849, 1801, 2083, 2417
AspS9I	GGNCC	2 cut(s)	1262, 1833
AsuC2I	CCSGG	2 cut(s)	281, 1941
AsuHPI	GGTGA	6 cut(s)	344, 376, 760, 1483, 1660, 2182
AsuII	TTCGAA	1 cut(s)	1167
AvaI	CYCGRG	1 cut(s)	2281
AvaII	GGWCC	2 cut(s)	1262, 1833
AvrII	CCTAGG	1 cut(s)	833
AxyI	CCTNAGG	1 cut(s)	360
BalI	TGGCCA	1 cut(s)	2113
BamHI	GGATCC	1 cut(s)	249
BanI	GGYRCC	1 cut(s)	1767
BanII	GRGCYC	2 cut(s)	401, 2150
BauI	CACGAG	3 cut(s)	233, 244, 2403
BbrPI	CACGTG	3 cut(s)	1100, 1959, 2058
Bbv12I	GWGCWC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
BbvI	GCAGC	9 cut(s)	259, 463, 628, 671, 1094, 1184, 1312, 2274, 2479
BceAI	ACGGC	6 cut(s)	64, 73, 334, 355, 579, 834
BcgI	CGANNNNNNTGC	6 cut(s)	1272, 1306, 1745, 1779, 2082, 2116
BciT130I	CCWGG	1 cut(s)	1177
BclI	TGATCA	2 cut(s)	1488, 2304
BcnI	CCSGG	2 cut(s)	281, 1941
BcoDI	GTCTC	8 cut(s)	112, 177, 436, 962, 1877, 1967, 2014, 2472
BfaI	CTAG	5 cut(s)	650, 702, 834, 908, 1346
BfmI	CTRYAG	4 cut(s)	1160, 1326, 1408, 1897
BfoI	RGCGCY	3 cut(s)	829, 850, 2084
BfuAI	ACCTGC	1 cut(s)	1408
BglI	GCCNNNNNGGC	1 cut(s)	473
BglII	AGATCT	1 cut(s)	2277
BlnI	CCTAGG	1 cut(s)	833
BmcAI	AGTACT	3 cut(s)	741, 1741, 2340
Bme1390I	CCNGG	3 cut(s)	281, 1177, 1941
Bme18I	GGWCC	2 cut(s)	1262, 1833
BmeT110I	CYCGRG	1 cut(s)	2281
BmgT120I	GGNCC	2 cut(s)	1262, 1833
BmiI	GGNNCC	7 cut(s)	51, 129, 251, 1264, 1338, 1769, 2546
BmrFI	CCNGG	3 cut(s)	281, 1177, 1941
BplI	GAGNNNNNCTC	4 cut(s)	227, 259, 757, 789
BpmI	CTGGAG	3 cut(s)	23, 146, 1688
Bpu14I	TTCGAA	1 cut(s)	1167
BpuMI	CCSGG	2 cut(s)	281, 1941
BsaAI	YACGTR	3 cut(s)	1100, 1959, 2058
BsaBI	GATNNNNATC	2 cut(s)	2276, 2636
BsaHI	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
BsaI	GGTCTC	1 cut(s)	436
BsaJI	CCNNGG	8 cut(s)	833, 857, 1176, 1836, 1939, 2022, 2108, 2487
BsaWI	WCCGGW	1 cut(s)	1333
BsaXI	ACNNNNNCTCC	6 cut(s)	1201, 1231, 2415, 2445, 2581, 2611
Bsc4I	CCNNNNNNNGG	5 cut(s)	46, 1311, 1645, 2012, 2120
Bse118I	RCCGGY	2 cut(s)	454, 1904
Bse1I	ACTGG	4 cut(s)	40, 129, 581, 2346
Bse21I	CCTNAGG	1 cut(s)	360
Bse8I	GATNNNNATC	2 cut(s)	2276, 2636
BseBI	CCWGG	1 cut(s)	1177
BseDI	CCNNGG	8 cut(s)	833, 857, 1176, 1836, 1939, 2022, 2108, 2487
BseGI	GGATG	8 cut(s)	430, 954, 1060, 1198, 1597, 2253, 2350, 2483
BseJI	GATNNNNATC	2 cut(s)	2276, 2636
BseLI	CCNNNNNNNGG	5 cut(s)	46, 1311, 1645, 2012, 2120
BseMII	CTCAG	2 cut(s)	351, 2464
BseNI	ACTGG	4 cut(s)	40, 129, 581, 2346
BseRI	GAGGAG	6 cut(s)	26, 202, 205, 1696, 1865, 1868
BseX3I	CGGCCG	1 cut(s)	267
BseXI	GCAGC	9 cut(s)	259, 463, 628, 671, 1094, 1184, 1312, 2274, 2479
BseYI	CCCAGC	1 cut(s)	1989
Bsh1236I	CGCG	4 cut(s)	171, 266, 1801, 1870
Bsh1285I	CGRYCG	2 cut(s)	57, 270
BshFI	GGCC	3 cut(s)	269, 1955, 2113
BshNI	GGYRCC	1 cut(s)	1767
BsiEI	CGRYCG	2 cut(s)	57, 270
BsiHKAI	GWGCWC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
BsiHKCI	CYCGRG	1 cut(s)	2281
BsiSI	CCGG	7 cut(s)	280, 455, 584, 1334, 1905, 1941, 2013
BslI	CCNNNNNNNGG	5 cut(s)	46, 1311, 1645, 2012, 2120
BsmAI	GTCTC	8 cut(s)	112, 177, 436, 962, 1877, 1967, 2014, 2472
BsmBI	CGTCTC	4 cut(s)	112, 1877, 2014, 2472
BsmI	GAATGC	3 cut(s)	1449, 1559, 2328
BsnI	GGCC	3 cut(s)	269, 1955, 2113
Bso31I	GGTCTC	1 cut(s)	436
BsoBI	CYCGRG	1 cut(s)	2281
Bsp119I	TTCGAA	1 cut(s)	1167
Bsp1286I	GDGCHC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
Bsp19I	CCATGG	2 cut(s)	857, 2022
BspANI	GGCC	3 cut(s)	269, 1955, 2113
BspCNI	CTCAG	2 cut(s)	352, 2463
BspFNI	CGCG	4 cut(s)	171, 266, 1801, 1870
BspHI	TCATGA	1 cut(s)	2634
BspLI	GGNNCC	7 cut(s)	51, 129, 251, 1264, 1338, 1769, 2546
BspMAI	CTGCAG	2 cut(s)	1164, 1330
BspMI	ACCTGC	1 cut(s)	1408
BspPI	GGATC	7 cut(s)	244, 257, 395, 579, 1129, 1190, 1443
BspT104I	TTCGAA	1 cut(s)	1167
BspT107I	GGYRCC	1 cut(s)	1767
BspTNI	GGTCTC	1 cut(s)	436
BsrFI	RCCGGY	2 cut(s)	454, 1904
BsrI	ACTGG	4 cut(s)	40, 129, 581, 2346
BssAI	RCCGGY	2 cut(s)	454, 1904
BssECI	CCNNGG	8 cut(s)	833, 857, 1176, 1836, 1939, 2022, 2108, 2487
BssNAI	GTATAC	1 cut(s)	1130
BssNI	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
BssSI	CACGAG	3 cut(s)	233, 244, 2403
BssT1I	CCWWGG	5 cut(s)	833, 857, 2022, 2108, 2487
Bst1107I	GTATAC	1 cut(s)	1130
Bst2BI	CACGAG	3 cut(s)	233, 244, 2403
Bst2UI	CCWGG	1 cut(s)	1177
Bst4CI	ACNGT	4 cut(s)	973, 1003, 1515, 1744
Bst6I	CTCTTC	1 cut(s)	29
BstACI	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
BstAPI	GCANNNNNTGC	1 cut(s)	1214
BstBAI	YACGTR	3 cut(s)	1100, 1959, 2058
BstBI	TTCGAA	1 cut(s)	1167
BstC8I	GCNNGC	6 cut(s)	682, 830, 954, 1184, 1813, 2647
BstDEI	CTNAG	2 cut(s)	360, 2450
BstDSI	CCRYGG	2 cut(s)	857, 2022
BstF5I	GGATG	8 cut(s)	430, 954, 1060, 1198, 1597, 2253, 2350, 2483
BstFNI	CGCG	4 cut(s)	171, 266, 1801, 1870
BstH2I	RGCGCY	3 cut(s)	829, 850, 2084
BstHHI	GCGC	7 cut(s)	173, 557, 828, 849, 1801, 2083, 2417
BstMAI	GTCTC	8 cut(s)	112, 177, 436, 962, 1877, 1967, 2014, 2472
BstMCI	CGRYCG	2 cut(s)	57, 270
BstNI	CCWGG	1 cut(s)	1177
BstNSI	RCATGY	4 cut(s)	684, 956, 2020, 2554
BstSCI	CCNGG	3 cut(s)	279, 1175, 1939
BstSFI	CTRYAG	4 cut(s)	1160, 1326, 1408, 1897
BstUI	CGCG	4 cut(s)	171, 266, 1801, 1870
BstV1I	GCAGC	9 cut(s)	259, 463, 628, 671, 1094, 1184, 1312, 2274, 2479
BstX2I	RGATCY	2 cut(s)	249, 2277
BstYI	RGATCY	2 cut(s)	249, 2277
BstZ17I	GTATAC	1 cut(s)	1130
BstZI	CGGCCG	1 cut(s)	267
Bsu36I	CCTNAGG	1 cut(s)	360
BsuRI	GGCC	3 cut(s)	269, 1955, 2113
BtgI	CCRYGG	2 cut(s)	857, 2022
BtgZI	GCGATG	1 cut(s)	1845
BtsCI	GGATG	8 cut(s)	430, 954, 1060, 1198, 1597, 2253, 2350, 2483
BtsIMutI	CAGTG	1 cut(s)	574
BveI	ACCTGC	1 cut(s)	1408
Cac8I	GCNNGC	6 cut(s)	682, 830, 954, 1184, 1813, 2647
CciI	TCATGA	1 cut(s)	2634
CciNI	GCGGCCGC	1 cut(s)	267
CfoI	GCGC	7 cut(s)	173, 557, 828, 849, 1801, 2083, 2417
Cfr10I	RCCGGY	2 cut(s)	454, 1904
Cfr13I	GGNCC	2 cut(s)	1262, 1833
CseI	GACGC	6 cut(s)	429, 1859, 1903, 1985, 2016, 2463
Csp6I	GTAC	7 cut(s)	82, 740, 756, 1624, 1740, 1768, 2339
CviQI	GTAC	7 cut(s)	82, 740, 756, 1624, 1740, 1768, 2339
DdeI	CTNAG	2 cut(s)	360, 2450
DraIII	CACNNNGTG	1 cut(s)	2388
EaeI	YGGCCR	3 cut(s)	267, 1953, 2111
EagI	CGGCCG	1 cut(s)	267
Eam1104I	CTCTTC	1 cut(s)	29
EarI	CTCTTC	1 cut(s)	29
EciI	GGCGGA	2 cut(s)	431, 2026
Ecl136II	GAGCTC	2 cut(s)	399, 2148
EclXI	CGGCCG	1 cut(s)	267
Eco130I	CCWWGG	5 cut(s)	833, 857, 2022, 2108, 2487
Eco24I	GRGCYC	2 cut(s)	401, 2150
Eco31I	GGTCTC	1 cut(s)	436
Eco47I	GGWCC	2 cut(s)	1262, 1833
Eco52I	CGGCCG	1 cut(s)	267
Eco53kI	GAGCTC	2 cut(s)	399, 2148
Eco57I	CTGAAG	2 cut(s)	398, 1640
Eco72I	CACGTG	3 cut(s)	1100, 1959, 2058
Eco81I	CCTNAGG	1 cut(s)	360
Eco88I	CYCGRG	1 cut(s)	2281
EcoICRI	GAGCTC	2 cut(s)	399, 2148
EcoO109I	RGGNCCY	1 cut(s)	1833
EcoRI	GAATTC	1 cut(s)	293
EcoRII	CCWGG	1 cut(s)	1175
EcoT14I	CCWWGG	5 cut(s)	833, 857, 2022, 2108, 2487
EcoT22I	ATGCAT	2 cut(s)	682, 954
EcoT38I	GRGCYC	2 cut(s)	401, 2150
ErhI	CCWWGG	5 cut(s)	833, 857, 2022, 2108, 2487
Esp3I	CGTCTC	4 cut(s)	112, 1877, 2014, 2472
FauI	CCCGC	1 cut(s)	2237
FauNDI	CATATG	1 cut(s)	1036
FbaI	TGATCA	2 cut(s)	1488, 2304
FblI	GTMKAC	2 cut(s)	1129, 2052
FokI	GGATG	8 cut(s)	437, 961, 1067, 1205, 1604, 2260, 2357, 2470
FriOI	GRGCYC	2 cut(s)	401, 2150
FspBI	CTAG	5 cut(s)	650, 702, 834, 908, 1346
FspI	TGCGCA	1 cut(s)	2416
GlaI	GCGC	7 cut(s)	172, 556, 827, 848, 1800, 2082, 2416
GsaI	CCCAGC	1 cut(s)	1993
GsuI	CTGGAG	3 cut(s)	23, 146, 1688
HaeII	RGCGCY	3 cut(s)	829, 850, 2084
HaeIII	GGCC	3 cut(s)	269, 1955, 2113
HapII	CCGG	7 cut(s)	280, 455, 584, 1334, 1905, 1941, 2013
HgaI	GACGC	6 cut(s)	429, 1859, 1903, 1985, 2016, 2463
HhaI	GCGC	7 cut(s)	173, 557, 828, 849, 1801, 2083, 2417
Hin1I	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
Hin6I	GCGC	7 cut(s)	171, 555, 826, 847, 1799, 2081, 2415
HinP1I	GCGC	7 cut(s)	171, 555, 826, 847, 1799, 2081, 2415
HincII	GTYRAC	1 cut(s)	2053
HindII	GTYRAC	1 cut(s)	2053
HindIII	AAGCTT	2 cut(s)	303, 610
HinfI	GANTC	8 cut(s)	62, 751, 1747, 1944, 2033, 2129, 2176, 2188
HpaII	CCGG	7 cut(s)	280, 455, 584, 1334, 1905, 1941, 2013
HphI	GGTGA	6 cut(s)	344, 376, 760, 1483, 1660, 2182
Hpy166II	GTNNAC	4 cut(s)	84, 1130, 2053, 2476
Hpy8I	GTNNAC	4 cut(s)	84, 1130, 2053, 2476
Hpy99I	CGWCG	9 cut(s)	445, 1291, 1294, 1828, 1831, 1919, 1979, 2032, 2054
HpyCH4III	ACNGT	4 cut(s)	973, 1003, 1515, 1744
HpyCH4IV	ACGT	9 cut(s)	105, 1099, 1257, 1286, 1826, 1958, 1999, 2057, 2465
HpyF3I	CTNAG	2 cut(s)	360, 2450
HpySE526I	ACGT	9 cut(s)	105, 1099, 1257, 1286, 1826, 1958, 1999, 2057, 2465
Hsp92I	GRCGYC	6 cut(s)	440, 1826, 1914, 1977, 2027, 2455
HspAI	GCGC	7 cut(s)	171, 555, 826, 847, 1799, 2081, 2415
KpnI	GGTACC	1 cut(s)	1771
Ksp22I	TGATCA	2 cut(s)	1488, 2304
LmnI	GCTCC	9 cut(s)	49, 127, 823, 844, 1146, 1222, 1998, 2123, 2544
Lsp1109I	GCAGC	9 cut(s)	259, 463, 628, 671, 1094, 1184, 1312, 2274, 2479
MaeI	CTAG	5 cut(s)	650, 702, 834, 908, 1346
MaeII	ACGT	9 cut(s)	105, 1099, 1257, 1286, 1826, 1958, 1999, 2057, 2465
MaeIII	GTNAC	9 cut(s)	90, 499, 704, 748, 815, 903, 1918, 2567, 2604
MboII	GAAGA	6 cut(s)	16, 1115, 1248, 2185, 2233, 2287
MfeI	CAATTG	1 cut(s)	1212
MflI	RGATCY	2 cut(s)	249, 2277
MhlI	GDGCHC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
MlsI	TGGCCA	1 cut(s)	2113
MluNI	TGGCCA	1 cut(s)	2113
MlyI	GAGTC	2 cut(s)	745, 1953
MmeI	TCCRAC	6 cut(s)	1240, 1998, 2010, 2020, 2413, 2522
Mox20I	TGGCCA	1 cut(s)	2113
Mph1103I	ATGCAT	2 cut(s)	682, 954
MscI	TGGCCA	1 cut(s)	2113
MseI	TTAA	7 cut(s)	591, 812, 885, 942, 1226, 1380, 1445
MslI	CAYNNNNRTG	4 cut(s)	957, 1191, 1592, 2021
Msp20I	TGGCCA	1 cut(s)	2113
MspA1I	CMGCKG	1 cut(s)	272
MspI	CCGG	7 cut(s)	280, 455, 584, 1334, 1905, 1941, 2013
MspR9I	CCNGG	3 cut(s)	281, 1177, 1941
MunI	CAATTG	1 cut(s)	1212
Mva1269I	GAATGC	3 cut(s)	1449, 1559, 2328
MvaI	CCWGG	1 cut(s)	1177
MvnI	CGCG	4 cut(s)	171, 266, 1801, 1870
NciI	CCSGG	2 cut(s)	281, 1941
NcoI	CCATGG	2 cut(s)	857, 2022
NdeI	CATATG	1 cut(s)	1036
NlaIV	GGNNCC	7 cut(s)	51, 129, 251, 1264, 1338, 1769, 2546
NmeAIII	GCCGAG	2 cut(s)	413, 1817
NmuCI	GTSAC	6 cut(s)	90, 704, 748, 815, 1918, 2604
NotI	GCGGCCGC	1 cut(s)	267
NsbI	TGCGCA	1 cut(s)	2416
NsiI	ATGCAT	2 cut(s)	682, 954
NspI	RCATGY	4 cut(s)	684, 956, 2020, 2554
NspV	TTCGAA	1 cut(s)	1167
PaeI	GCATGC	2 cut(s)	684, 956
PaeR7I	CTCGAG	1 cut(s)	2281
PagI	TCATGA	1 cut(s)	2634
PcsI	WCGNNNNNNNCGW	1 cut(s)	2049
PctI	GAATGC	3 cut(s)	1449, 1559, 2328
PfeI	GAWTC	6 cut(s)	62, 1747, 2033, 2129, 2176, 2188
PflMI	CCANNNNNTGG	1 cut(s)	2120
Ple19I	CGATCG	1 cut(s)	57
PleI	GAGTC	2 cut(s)	745, 1952
PmaCI	CACGTG	3 cut(s)	1100, 1959, 2058
PmlI	CACGTG	3 cut(s)	1100, 1959, 2058
PpsI	GAGTC	2 cut(s)	745, 1952
Ppu21I	YACGTR	3 cut(s)	1100, 1959, 2058
PpuMI	RGGWCCY	1 cut(s)	1833
PsiI	TTATAA	1 cut(s)	1007
Psp124BI	GAGCTC	2 cut(s)	401, 2150
Psp1406I	AACGTT	1 cut(s)	1257
Psp5II	RGGWCCY	1 cut(s)	1833
Psp6I	CCWGG	1 cut(s)	1175
PspCI	CACGTG	3 cut(s)	1100, 1959, 2058
PspFI	CCCAGC	1 cut(s)	1989
PspGI	CCWGG	1 cut(s)	1175
PspN4I	GGNNCC	7 cut(s)	51, 129, 251, 1264, 1338, 1769, 2546
PspPI	GGNCC	2 cut(s)	1262, 1833
PspPPI	RGGWCCY	1 cut(s)	1833
PstI	CTGCAG	2 cut(s)	1164, 1330
PsuI	RGATCY	2 cut(s)	249, 2277
PvuI	CGATCG	1 cut(s)	57
RsaI	GTAC	7 cut(s)	83, 741, 757, 1625, 1741, 1769, 2340
RsaNI	GTAC	7 cut(s)	82, 740, 756, 1624, 1740, 1768, 2339
RseI	CAYNNNNRTG	4 cut(s)	957, 1191, 1592, 2021
SacI	GAGCTC	2 cut(s)	401, 2150
SalI	GTCGAC	1 cut(s)	2051
SaqAI	TTAA	7 cut(s)	591, 812, 885, 942, 1226, 1380, 1445
Sau96I	GGNCC	2 cut(s)	1262, 1833
ScaI	AGTACT	3 cut(s)	741, 1741, 2340
SchI	GAGTC	2 cut(s)	745, 1953
ScrFI	CCNGG	3 cut(s)	281, 1177, 1941
SduI	GDGCHC	6 cut(s)	101, 205, 401, 2150, 2451, 2532
SfcI	CTRYAG	4 cut(s)	1160, 1326, 1408, 1897
Sfr274I	CTCGAG	1 cut(s)	2281
SfuI	TTCGAA	1 cut(s)	1167
SgrAI	CRCCGGYG	1 cut(s)	1904
SinI	GGWCC	2 cut(s)	1262, 1833
SlaI	CTCGAG	1 cut(s)	2281
SmiMI	CAYNNNNRTG	4 cut(s)	957, 1191, 1592, 2021
SmlI	CTYRAG	1 cut(s)	2281
SmoI	CTYRAG	1 cut(s)	2281
SphI	GCATGC	2 cut(s)	684, 956
SspI	AATATT	3 cut(s)	694, 1279, 1547
SspMI	CTAG	5 cut(s)	650, 702, 834, 908, 1346
SstI	GAGCTC	2 cut(s)	401, 2150
StyD4I	CCNGG	3 cut(s)	279, 1175, 1939
StyI	CCWWGG	5 cut(s)	833, 857, 2022, 2108, 2487
TaaI	ACNGT	4 cut(s)	973, 1003, 1515, 1744
TaiI	ACGT	9 cut(s)	108, 1102, 1260, 1289, 1829, 1961, 2002, 2060, 2468
TatI	WGTACW	4 cut(s)	739, 1623, 1739, 2338
TfiI	GAWTC	6 cut(s)	62, 1747, 2033, 2129, 2176, 2188
Tru1I	TTAA	7 cut(s)	591, 812, 885, 942, 1226, 1380, 1445
Tru9I	TTAA	7 cut(s)	591, 812, 885, 942, 1226, 1380, 1445
TscAI	CASTG	1 cut(s)	581
TseFI	GTSAC	6 cut(s)	90, 704, 748, 815, 1918, 2604
TseI	GCWGC	9 cut(s)	272, 476, 641, 684, 1082, 1172, 1325, 2287, 2492
Tsp45I	GTSAC	6 cut(s)	90, 704, 748, 815, 1918, 2604
TspDTI	ATGAA	6 cut(s)	819, 1071, 1442, 1698, 2234, 2288
TspGWI	ACGGA	1 cut(s)	100
TspRI	CASTG	1 cut(s)	581
Van91I	CCANNNNNTGG	1 cut(s)	2120
VpaK11BI	GGWCC	2 cut(s)	1262, 1833
XapI	RAATTY	4 cut(s)	293, 795, 1705, 2182
XbaI	TCTAGA	1 cut(s)	1345
XceI	RCATGY	4 cut(s)	684, 956, 2020, 2554
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2164, 2494
XhoI	CTCGAG	1 cut(s)	2281
XmaJI	CCTAGG	1 cut(s)	833
XmiI	GTMKAC	2 cut(s)	1129, 2052
XspI	CTAG	5 cut(s)	650, 702, 834, 908, 1346
ZraI	GACGTC	1 cut(s)	1827
ZrmI	AGTACT	3 cut(s)	741, 1741, 2340
Zsp2I	ATGCAT	2 cut(s)	682, 954

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.