Rw1G035130

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004592
Physical Location & Seq
Reverse (-)
62955995 .. 62957572
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035130.1

Sequence Viewer

Length: 702 bp
ATGCTCACCAATCCCATCCTCTCTATAAATACACCATCCCCCTCATCCTCATCCTCAAACCTCAATTTTCCCAATCAGTCCCTTACTCTCTCATCCCTACTCCAACTCAGCCTCATAACAATGGCTAGATCCTCCATTCTCTTTCTACTTCTTGCCGTCTTCGCCCTCACCGCCAACTCTGCTTCCACCACCACTGCCTCCAACTTCGTCAAAACCTCATGCAGCACCACCACCTACCCTGACCTCTGCGTTCAGTCCCTCTCATCATTCTCCACCGCGATCCAGCAAAGCCCTCGCCAGCTGGCCCAGACCGCCCTCTCCGTCAGCCTCACCCGAGCCCAGTTCGCCAAGTCCTTCGTCACCAAGCTGACCAAGTTCAAAGGTTTGAGCCACAAGGAGTACGAGGCCATCGACGACTGCCTTGAGGAGATGGGTGACAGCGTCGACCGGCTGAGCAAGTCCGTGGACGAGCTGAAGAAGATGGGGAAGTCATCCAAGGGCCAGGATTTCTTATGGCACATGAGCAATGTCGAGACTTGGGTCAGCGCTGCGTTGACTGACGACAACACTTGCCTTGATGGGTTCTCCGGGAAGGCCATGAATGGCAAGATCAAGGCCTCCATTAGAGCTCAGGTGCTTAATGTTGCTAAGGTTACTAGTAATGCTCTGGCCCTTTGCAACCACTTTGCCTCCAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0005575 GO:0005576 GO:0008150 GO:0009987 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0048046 GO:0050790 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

233

Amino Acids

25.13

Weight (kDa)

8.97

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 225 8.1e-42 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 444
AccII CGCG 1 cut(s) 278
AciI CCGC 3 cut(s) 171, 276, 312
AclWI GGATC 2 cut(s) 123, 274
AcuI CTGAAG 1 cut(s) 494
AfaI GTAC 2 cut(s) 401, 698
AfeI AGCGCT 1 cut(s) 547
AgsI TTSAA 1 cut(s) 379
AhlI ACTAGT 1 cut(s) 656
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 4 cut(s) 301, 367, 472, 629
AluI AGCT 4 cut(s) 301, 367, 472, 629
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 2 cut(s) 123, 274
Ama87I CYCGRG 1 cut(s) 333
Aor51HI AGCGCT 1 cut(s) 547
AoxI GGCC 6 cut(s) 303, 405, 499, 594, 615, 669
ApeKI GCWGC 2 cut(s) 222, 548
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 304, 499, 670
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 4 cut(s) 160, 322, 352, 446
AvaI CYCGRG 1 cut(s) 333
BanII GRGCYC 2 cut(s) 340, 631
BbsI GAAGAC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 2 cut(s) 234, 535
BccI CCATC 6 cut(s) 23, 43, 416, 424, 475, 572
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 503
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 1 cut(s) 527
BcuI ACTAGT 1 cut(s) 656
BfaI CTAG 2 cut(s) 126, 657
BfoI RGCGCY 1 cut(s) 549
BisI GCNGC 2 cut(s) 223, 549
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 2 cut(s) 224, 550
BmcAI AGTACT 1 cut(s) 698
Bme1390I CCNGG 2 cut(s) 503, 589
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 3 cut(s) 304, 499, 670
BmrFI CCNGG 2 cut(s) 503, 589
BmrI ACTGGG 1 cut(s) 334
BmuI ACTGGG 1 cut(s) 334
BoxI GACNNNNGTC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 151
Bpu10I CCTNAGC 2 cut(s) 630, 648
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 589
BsaJI CCNNGG 2 cut(s) 462, 495
BsaXI ACNNNNNCTCC 1 cut(s) 674
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 2 cut(s) 462, 495
BseGI GGATG 6 cut(s) 15, 35, 44, 50, 92, 491
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 3 cut(s) 121, 443, 644
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 2 cut(s) 234, 535
Bsh1236I CGCG 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 448
BshFI GGCC 6 cut(s) 305, 407, 501, 596, 617, 671
BsiEI CGRYCG 1 cut(s) 448
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 448, 588
BslFI GGGAC 2 cut(s) 64, 241
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 2 cut(s) 64, 241
BsnI GGCC 6 cut(s) 305, 407, 501, 596, 617, 671
BsoBI CYCGRG 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 340, 631
Bsp143I GATC 3 cut(s) 128, 279, 609
Bsp1720I GCTNAGC 1 cut(s) 452
BspACI CCGC 3 cut(s) 171, 276, 312
BspANI GGCC 6 cut(s) 305, 407, 501, 596, 617, 671
BspCNI CTCAG 3 cut(s) 120, 444, 643
BspFNI CGCG 1 cut(s) 278
BspPI GGATC 2 cut(s) 123, 274
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 462, 495
BssMI GATC 3 cut(s) 128, 279, 609
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 299, 303
BstDEI CTNAG 4 cut(s) 107, 452, 630, 648
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 6 cut(s) 15, 35, 44, 50, 92, 491
BstFNI CGCG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 3 cut(s) 131, 282, 612
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 3 cut(s) 128, 279, 609
BstMCI CGRYCG 1 cut(s) 448
BstMWI GCNNNNNNNGC 5 cut(s) 161, 170, 179, 311, 344
BstNI CCWGG 1 cut(s) 503
BstPAI GACNNNNGTC 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 501, 587
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 234, 535
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 6 cut(s) 305, 407, 501, 596, 617, 671
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 6 cut(s) 15, 35, 44, 50, 92, 491
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 299, 303
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 3 cut(s) 304, 499, 670
CseI GACGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 400, 697
CviAII CATG 3 cut(s) 219, 520, 598
CviQI GTAC 2 cut(s) 400, 697
DdeI CTNAG 4 cut(s) 107, 452, 630, 648
DpnI GATC 3 cut(s) 130, 281, 611
DpnII GATC 3 cut(s) 128, 279, 609
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 2 cut(s) 340, 631
Eco47III AGCGCT 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 2 cut(s) 340, 631
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 222, 523, 601
FaiI YATR 6 cut(s) 26, 116, 220, 514, 521, 599
FaqI GGGAC 2 cut(s) 64, 241
FatI CATG 3 cut(s) 218, 519, 597
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 223, 549
FokI GGATG 6 cut(s) 2, 22, 31, 37, 79, 478
FriOI GRGCYC 2 cut(s) 340, 631
Fsp4HI GCNGC 2 cut(s) 223, 549
FspBI CTAG 2 cut(s) 126, 657
GlaI GCGC 1 cut(s) 547
GluI GCNGC 2 cut(s) 223, 549
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 6 cut(s) 305, 407, 501, 596, 617, 671
HapII CCGG 2 cut(s) 448, 588
HgaI GACGC 1 cut(s) 430
HhaI GCGC 1 cut(s) 548
Hin1II CATG 3 cut(s) 222, 523, 601
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 2 cut(s) 445, 555
HindII GTYRAC 2 cut(s) 445, 555
HpaII CCGG 2 cut(s) 448, 588
HphI GGTGA 4 cut(s) 160, 322, 352, 446
Hpy166II GTNNAC 3 cut(s) 445, 466, 555
Hpy188III TCNNGA 1 cut(s) 532
Hpy8I GTNNAC 3 cut(s) 445, 466, 555
Hpy99I CGWCG 2 cut(s) 416, 446
HpyAV CCTTC 2 cut(s) 364, 586
HpyCH4V TGCA 2 cut(s) 222, 678
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 170, 179, 311, 344
HpyF3I CTNAG 4 cut(s) 107, 452, 630, 648
Hsp92II CATG 3 cut(s) 222, 523, 601
HspAI GCGC 1 cut(s) 546
Kzo9I GATC 3 cut(s) 128, 279, 609
Lsp1109I GCAGC 2 cut(s) 234, 535
MaeI CTAG 2 cut(s) 126, 657
MaeIII GTNAC 3 cut(s) 358, 434, 652
MalI GATC 3 cut(s) 130, 281, 611
MboI GATC 3 cut(s) 128, 279, 609
MboII GAAGA 3 cut(s) 151, 487, 490
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 2 cut(s) 340, 631
MluCI AATT 1 cut(s) 64
MmeI TCCRAC 2 cut(s) 127, 225
MseI TTAA 1 cut(s) 639
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 2 cut(s) 448, 588
MspR9I CCNGG 2 cut(s) 503, 589
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 5 cut(s) 161, 170, 179, 311, 344
NciI CCSGG 1 cut(s) 589
NdeII GATC 3 cut(s) 128, 279, 609
NlaIII CATG 3 cut(s) 222, 523, 601
NmuCI GTSAC 2 cut(s) 358, 434
PceI AGGCCT 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 440
PfoI TCCNGGA 1 cut(s) 587
PkrI GCNGC 2 cut(s) 224, 550
PshAI GACNNNNGTC 1 cut(s) 539
Psp124BI GAGCTC 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspPI GGNCC 3 cut(s) 304, 499, 670
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 2 cut(s) 401, 698
RsaNI GTAC 2 cut(s) 400, 697
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 631
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 639
SatI GCNGC 2 cut(s) 223, 549
Sau3AI GATC 3 cut(s) 128, 279, 609
Sau96I GGNCC 3 cut(s) 304, 499, 670
ScaI AGTACT 1 cut(s) 698
ScrFI CCNGG 2 cut(s) 503, 589
SduI GDGCHC 2 cut(s) 340, 631
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 656
Sse9I AATT 1 cut(s) 64
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 3 cut(s) 171, 276, 312
SspMI CTAG 2 cut(s) 126, 657
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 2 cut(s) 501, 587
StyI CCWWGG 1 cut(s) 495
TaqI TCGA 3 cut(s) 411, 444, 531
TasI AATT 1 cut(s) 64
TatI WGTACW 1 cut(s) 696
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 358, 434
TseI GCWGC 2 cut(s) 222, 548
Tsp45I GTSAC 2 cut(s) 358, 434
TspDTI ATGAA 1 cut(s) 614
TspGWI ACGGA 2 cut(s) 310, 451
TspRI CASTG 1 cut(s) 199
Tth111I GACNNNGTC 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 2 cut(s) 126, 657
ZrmI AGTACT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.