AT1G67830

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003753
Physical Location & Seq
Reverse (-)
25431549 .. 25433091
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67830.1

Sequence Viewer

Length: 1119 bp
ATGAATCCAATCCTATCTTCTTTGTTTGCTCTCTCTTTGCTTTCTTCACTATCACCATCAACACATGCCCATCAATGCCATTTCCCAGCAATCTTTAACTTTGGTGACTCTAACTCCGATACCGGCGGTCTCTCCGCTGCTTTTGGCCAAGCTGGTCCTCCTCACGGCTCTTCCTTCTTCGGCTCACCCGCGGGACGATACTGCGATGGTCGCCTCGTCATTGACTTCATAGCGGAGAGTCTTGGATTACCATATCTAAGCGCGTTTCTAGACTCCGTTGGTTCAAACTTCAGCCACGGCGCTAACTTCGCTACCGCGGGATCTCCGATAAGAGCTCTTAACTCTACTCTACGTCAAAGTGGATTCAGCCCATTTTCACTCGATGTACAGTTCGTACAATTCTATAACTTCCACAACAGATCTCAAACTGTTCGTAGTCGTGGAGGAGTCTACAAGACAATGTTGCCTGAATCCGACAGCTTCTCAAAAGCTTTGTACACTTTTGACATTGGTCAGAACGATCTCACAGCAGGTTACTTCGCTAATAAAACCGTAGAACAAGTTGAAACCGAAGTTCCTGAGATCATAAGTCAGTTCATGAATGCTATCAAGAATATTTATGGACAAGGAGGGAGATACTTCTGGATTCATAACACTGGACCTATTGGTTGTTTAGCTTATGTGATTGAACGGTTTCCGAATAAAGCATCAGACTTTGATTCACATGGATGTGTTTCTCCTTTGAACCACTTAGCTCAACAATTCAATCACGCTTTGAAACAAGCTGTGATCGAGCTAAGAAGTTCTCTATCTGAAGCAGCCATCACTTATGTAGATGTCTACTCTCTCAAGCATGAGTTGTTTGTTCACGCTCAGGGTCACGGGTTTAAGGGATCGTTGGTTTCGTGTTGTGGACATGGAGGGAAGTATAACTACAACAAGGGTATCGGATGTGGGATGAAGAAGATTGTGAAAGGTAAAGAGGTTTACATTGGAAAGCCGTGCGATGAGCCTGATAAGGCTGTTGTGTGGGACGGTGTACACTTCACGCAGGCTGCTAACAAATTCATTTTCGATAAGATTGCTCCAGGGTTAAGCAAGGCCTGTAAAAGGCAGTGA

Protein Analysis

372

Amino Acids

40.46

Weight (kDa)

8.29

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 361 1.5e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccI GTMKAC 2 cut(s) 450, 840
AccII CGCG 3 cut(s) 191, 263, 317
AciI CCGC 7 cut(s) 126, 135, 189, 191, 233, 315, 317
AclWI GGATC 2 cut(s) 328, 901
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 1 cut(s) 1064
AcuI CTGAAG 2 cut(s) 274, 834
AfaI GTAC 4 cut(s) 387, 396, 497, 1041
AfiI CCNNNNNNNGG 2 cut(s) 164, 1110
AgsI TTSAA 6 cut(s) 285, 566, 689, 745, 766, 778
AjnI CCWGG 1 cut(s) 1087
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AluBI AGCT 8 cut(s) 152, 335, 480, 491, 677, 755, 785, 796
AluI AGCT 8 cut(s) 152, 335, 480, 491, 677, 755, 785, 796
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 2 cut(s) 328, 901
AoxI GGCC 2 cut(s) 145, 1101
ApeKI GCWGC 3 cut(s) 137, 818, 1055
ApoI RAATTY 1 cut(s) 1064
Asp700I GAANNNNTTC 1 cut(s) 693
AspLEI GCGC 2 cut(s) 263, 302
AspS9I GGNCC 2 cut(s) 155, 659
AsuHPI GGTGA 3 cut(s) 45, 116, 177
AvaII GGWCC 2 cut(s) 155, 659
BaeI ACNNNNGTAYC 2 cut(s) 928, 961
BalI TGGCCA 1 cut(s) 147
BanII GRGCYC 1 cut(s) 337
BarI GAAGNNNNNNTAC 2 cut(s) 917, 949
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 124, 830, 1042
BccI CCATC 4 cut(s) 64, 78, 200, 830
BceAI ACGGC 3 cut(s) 181, 313, 985
BcgI CGANNNNNNTGC 2 cut(s) 1064, 1098
BciT130I CCWGG 1 cut(s) 1089
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 521
BglII AGATCT 1 cut(s) 419
BisI GCNGC 3 cut(s) 138, 819, 1056
BlsI GCNGC 3 cut(s) 139, 820, 1057
Bme1390I CCNGG 1 cut(s) 1089
Bme18I GGWCC 2 cut(s) 155, 659
BmgT120I GGNCC 2 cut(s) 155, 659
BmrFI CCNGG 1 cut(s) 1089
BmsI GCATC 1 cut(s) 716
BoxI GACNNNNGTC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 1071
Bpu10I CCTNAGC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 833
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 4 cut(s) 189, 295, 315, 1088
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 1110
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 661
BseBI CCWGG 1 cut(s) 1089
BseDI CCNNGG 4 cut(s) 189, 295, 315, 1088
BseGI GGATG 3 cut(s) 734, 956, 963
BseLI CCNNNNNNNGG 2 cut(s) 164, 1110
BseMII CTCAG 2 cut(s) 570, 887
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 2 cut(s) 150, 459
BseXI GCAGC 3 cut(s) 124, 830, 1042
BseYI CCCAGC 1 cut(s) 85
Bsh1236I CGCG 3 cut(s) 191, 263, 317
BshFI GGCC 2 cut(s) 147, 1103
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 2 cut(s) 207, 1046
BslI CCNNNNNNNGG 2 cut(s) 164, 1110
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 2 cut(s) 207, 1046
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 147, 1103
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 337
Bsp1407I TGTACA 3 cut(s) 385, 495, 1039
Bsp143I GATC 6 cut(s) 320, 419, 520, 582, 789, 893
BspACI CCGC 7 cut(s) 126, 135, 189, 191, 233, 315, 317
BspANI GGCC 2 cut(s) 147, 1103
BspCNI CTCAG 2 cut(s) 571, 886
BspFNI CGCG 3 cut(s) 191, 263, 317
BspHI TCATGA 1 cut(s) 597
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 328, 901
BspQI GCTCTTC 1 cut(s) 175
BspTNI GGTCTC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 3 cut(s) 385, 495, 1039
BsrI ACTGG 1 cut(s) 661
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 189, 295, 315, 1088
BssMI GATC 6 cut(s) 320, 419, 520, 582, 789, 893
Bst2UI CCWGG 1 cut(s) 1089
Bst4CI ACNGT 5 cut(s) 390, 430, 553, 693, 1037
Bst6I CTCTTC 1 cut(s) 175
BstAUI TGTACA 3 cut(s) 385, 495, 1039
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 5 cut(s) 257, 579, 751, 797, 873
BstDSI CCRYGG 3 cut(s) 189, 295, 315
BstENI CCTNNNNNAGG 1 cut(s) 1108
BstF5I GGATG 3 cut(s) 734, 956, 963
BstFNI CGCG 3 cut(s) 191, 263, 317
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 263, 302
BstKTI GATC 6 cut(s) 323, 422, 523, 585, 792, 896
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 6 cut(s) 320, 419, 520, 582, 789, 893
BstMWI GCNNNNNNNGC 2 cut(s) 210, 308
BstNI CCWGG 1 cut(s) 1089
BstNSI RCATGY 1 cut(s) 68
BstPAI GACNNNNGTC 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 1087
BstUI CGCG 3 cut(s) 191, 263, 317
BstV1I GCAGC 3 cut(s) 124, 830, 1042
BstX2I RGATCY 2 cut(s) 320, 419
BstYI RGATCY 2 cut(s) 320, 419
BsuRI GGCC 2 cut(s) 147, 1103
BtgI CCRYGG 3 cut(s) 189, 295, 315
BtgZI GCGATG 2 cut(s) 219, 1020
BtsCI GGATG 3 cut(s) 734, 956, 963
BtsIMutI CAGTG 1 cut(s) 654
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 1053
CciI TCATGA 1 cut(s) 597
CfoI GCGC 2 cut(s) 263, 302
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 155, 659
Cfr42I CCGCGG 2 cut(s) 192, 318
Csp6I GTAC 4 cut(s) 386, 395, 496, 1040
CviAII CATG 5 cut(s) 65, 598, 725, 854, 917
CviQI GTAC 4 cut(s) 386, 395, 496, 1040
DdeI CTNAG 5 cut(s) 257, 579, 751, 797, 873
DpnI GATC 6 cut(s) 322, 421, 522, 584, 791, 895
DpnII GATC 6 cut(s) 320, 419, 520, 582, 789, 893
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 335
Eco147I AGGCCT 1 cut(s) 1103
Eco24I GRGCYC 1 cut(s) 337
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 155, 659
Eco53kI GAGCTC 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 274, 834
EcoICRI GAGCTC 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 1108
EcoRII CCWGG 1 cut(s) 1087
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 5 cut(s) 68, 601, 728, 857, 920
FaqI GGGAC 2 cut(s) 207, 1046
FatI CATG 5 cut(s) 64, 597, 724, 853, 916
FauI CCCGC 3 cut(s) 184, 196, 310
FblI GTMKAC 2 cut(s) 450, 840
Fnu4HI GCNGC 3 cut(s) 138, 819, 1056
FokI GGATG 3 cut(s) 741, 963, 970
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 3 cut(s) 138, 819, 1056
FspBI CTAG 1 cut(s) 269
GlaI GCGC 2 cut(s) 262, 301
GluI GCNGC 3 cut(s) 138, 819, 1056
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 1071
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 2 cut(s) 147, 1103
HapII CCGG 1 cut(s) 123
HhaI GCGC 2 cut(s) 263, 302
Hin1II CATG 5 cut(s) 68, 601, 728, 857, 920
Hin6I GCGC 2 cut(s) 261, 300
HinP1I GCGC 2 cut(s) 261, 300
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 9 cut(s) 4, 107, 238, 272, 363, 447, 470, 646, 719
HpaII CCGG 1 cut(s) 123
HphI GGTGA 3 cut(s) 45, 116, 177
Hpy166II GTNNAC 8 cut(s) 451, 498, 841, 868, 914, 988, 1040, 1042
Hpy188I TCNGA 8 cut(s) 118, 327, 475, 516, 699, 712, 814, 950
Hpy188III TCNNGA 5 cut(s) 269, 578, 598, 610, 643
Hpy8I GTNNAC 8 cut(s) 451, 498, 841, 868, 914, 988, 1040, 1042
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 5 cut(s) 390, 430, 553, 693, 1037
HpyCH4IV ACGT 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 308
HpyF3I CTNAG 5 cut(s) 257, 579, 751, 797, 873
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 5 cut(s) 68, 601, 728, 857, 920
HspAI GCGC 2 cut(s) 261, 300
KspI CCGCGG 2 cut(s) 192, 318
Kzo9I GATC 6 cut(s) 320, 419, 520, 582, 789, 893
LguI GCTCTTC 1 cut(s) 175
LmnI GCTCC 1 cut(s) 1090
Lsp1109I GCAGC 3 cut(s) 124, 830, 1042
LweI GCATC 1 cut(s) 716
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 3 cut(s) 104, 533, 878
MalI GATC 6 cut(s) 322, 421, 522, 584, 791, 895
MboI GATC 6 cut(s) 320, 419, 520, 582, 789, 893
MboII GAAGA 6 cut(s) 9, 36, 162, 169, 973, 976
MflI RGATCY 2 cut(s) 320, 419
MhlI GDGCHC 1 cut(s) 337
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 3 cut(s) 398, 761, 1064
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 4 cut(s) 101, 247, 266, 456
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 7 cut(s) 168, 171, 224, 437, 623, 914, 976
Mox20I TGGCCA 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 147
MseI TTAA 4 cut(s) 96, 339, 888, 1094
MslI CAYNNNNRTG 2 cut(s) 727, 729
Msp20I TGGCCA 1 cut(s) 147
MspA1I CMGCKG 3 cut(s) 137, 191, 317
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 1089
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 1089
MvnI CGCG 3 cut(s) 191, 263, 317
MwoI GCNNNNNNNGC 2 cut(s) 210, 308
NdeII GATC 6 cut(s) 320, 419, 520, 582, 789, 893
NlaIII CATG 5 cut(s) 68, 601, 728, 857, 920
NmuCI GTSAC 2 cut(s) 104, 878
NspI RCATGY 1 cut(s) 68
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 1103
PciSI GCTCTTC 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 902
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 5 cut(s) 4, 363, 470, 646, 719
PkrI GCNGC 3 cut(s) 139, 820, 1057
PleI GAGTC 4 cut(s) 101, 246, 266, 455
PpsI GAGTC 4 cut(s) 101, 246, 266, 455
PshAI GACNNNNGTC 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 1087
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 1087
PspPI GGNCC 2 cut(s) 155, 659
PsuI RGATCY 2 cut(s) 320, 419
RsaI GTAC 4 cut(s) 387, 396, 497, 1041
RsaNI GTAC 4 cut(s) 386, 395, 496, 1040
RseI CAYNNNNRTG 2 cut(s) 727, 729
SacI GAGCTC 1 cut(s) 337
SacII CCGCGG 2 cut(s) 192, 318
SapI GCTCTTC 1 cut(s) 175
SaqAI TTAA 4 cut(s) 96, 339, 888, 1094
SatI GCNGC 3 cut(s) 138, 819, 1056
Sau3AI GATC 6 cut(s) 320, 419, 520, 582, 789, 893
Sau96I GGNCC 2 cut(s) 155, 659
SchI GAGTC 4 cut(s) 101, 247, 266, 456
ScrFI CCNGG 1 cut(s) 1089
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 1 cut(s) 716
Sfr303I CCGCGG 2 cut(s) 192, 318
SgrBI CCGCGG 2 cut(s) 192, 318
SinI GGWCC 2 cut(s) 155, 659
SmiMI CAYNNNNRTG 2 cut(s) 727, 729
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
Sse9I AATT 3 cut(s) 398, 761, 1064
SseBI AGGCCT 1 cut(s) 1103
SsiI CCGC 7 cut(s) 126, 135, 189, 191, 233, 315, 317
SspI AATATT 1 cut(s) 616
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 337
StuI AGGCCT 1 cut(s) 1103
StyD4I CCNGG 1 cut(s) 1087
TaaI ACNGT 5 cut(s) 390, 430, 553, 693, 1037
TaiI ACGT 1 cut(s) 355
TaqI TCGA 3 cut(s) 381, 792, 1074
TasI AATT 3 cut(s) 398, 761, 1064
TatI WGTACW 3 cut(s) 385, 495, 1039
TfiI GAWTC 5 cut(s) 4, 363, 470, 646, 719
Tru1I TTAA 4 cut(s) 96, 339, 888, 1094
Tru9I TTAA 4 cut(s) 96, 339, 888, 1094
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 2 cut(s) 104, 878
TseI GCWGC 3 cut(s) 137, 818, 1055
Tsp45I GTSAC 2 cut(s) 104, 878
TspDTI ATGAA 7 cut(s) 17, 217, 586, 614, 638, 974, 1057
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 1 cut(s) 661
VpaK11BI GGWCC 2 cut(s) 155, 659
XagI CCTNNNNNAGG 1 cut(s) 1108
XapI RAATTY 1 cut(s) 1064
XbaI TCTAGA 1 cut(s) 268
XceI RCATGY 1 cut(s) 68
XmiI GTMKAC 2 cut(s) 450, 840
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.