RchiOBHm_Chr4g0433451

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003753
Physical Location & Seq
Forward (+)
57581214 .. 57583399
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40196

Sequence Viewer

Length: 1155 bp
ATGGAATCACATTCCTCAACAATACTAGCAGTTCTCACTCTTCTGGTGTCACTGTCTGCAGTTAGCTTGGCCTCAACTAATTCATGTAACTTCCCAGCCGTATTCAACTTCGGCGACTCAAACTCCGACACCGGTGGCTTGTCTGCAGTCTTCGGCCAGGCACGTTCACCTCACGGCGAGTCCTTCTTCCACGGCCCGGCTGGCCGGTACTGTGACGGCCGCCTCGTCATTGATTTTATAGCTAAAAGCCTTGGACTACCATATCTTAGCGCATATCTCGACTCGGTGGGAAGCAACTTCACTCACGGAGCCAACTTCGCGACGGCCGGATCGACCATCCGGCCTCAGAACACAACCATTCAACAGAGTGGGTTTAGTCCCATCTCCTTAAACGTTCAGTACAACGAGTTCTATGATTTTCTTCCTAGGTCCCAAGTTGCTCGGAATCGAGGTGGAGTTTTTGCACAACTAATGCCTAAAGCTGAGGATTTCTCTCGTGCCTTGTACACCTTCGATATTGGTCAGAACGACTTGACAGCCGGTTTGTTCTTGAACATGTCCGTCGCCCAAGTTAAAGCATTTGTTCCTGATGTACTCGATAACTTCAAAGACGTCGTCAAGTATGTACACAGTCAAGGAGGTAGATTTTTCTGGATACACAACACGGGACCGGTCGGTTGTCTACCCTATGTATTGGACCGCCTACCGGTCTTACCCTCGGAGGTGGACCGCCACGGGTGCGCCACACCTTTCAATGAACTTGCTCAGTTTTTCAACCGTGGATTAAAGCACGCTGTGGTCCAGTTAAGGAAAGAGCTACCTTCGGCTGCAATCACATATGTTGATGTTTACAAGGCCAAGTTCAACCTCATTAGCCAACCACAAAAGCATGGAGTTAAAGAACCGATAAGAGCATGTTGCGGCCATGGTGGGAAGTACAATTTCAATCCGCACCTTGGATGTGGAGGAAAGATCAAGGTTCATGGCAAAGAAATATTAGTGGGAAAGTCATGTAAAGACCCTTCGGTTTGGGTAAATTGGGATGGGGTACATTACACTCAAGCAGCTAACAAAAAGATCTTCGATCAGATTGTAGATGGTTCATTTTCGAACCCTCCAGTTCCTTTGAAAATGGCTTGCCATAAGCACCATTAA

Protein Analysis

384

Amino Acids

41.97

Weight (kDa)

8.86

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 364 1.2e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 615
AccI GTMKAC 1 cut(s) 682
AccII CGCG 1 cut(s) 320
AciI CCGC 5 cut(s) 220, 700, 730, 921, 950
AclI AACGTT 1 cut(s) 393
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 5 cut(s) 154, 202, 217, 324, 922
AcyI GRCGYC 1 cut(s) 612
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 7 cut(s) 209, 401, 506, 594, 627, 938, 1050
AfiI CCNNNNNNNGG 3 cut(s) 196, 706, 956
AflIII ACRYGT 1 cut(s) 555
AgeI ACCGGT 3 cut(s) 131, 670, 706
AgsI TTSAA 9 cut(s) 106, 362, 553, 607, 754, 775, 865, 946, 1129
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 5 cut(s) 66, 242, 482, 817, 1067
AluI AGCT 5 cut(s) 66, 242, 482, 817, 1067
AlwI GGATC 1 cut(s) 337
AoxI GGCC 9 cut(s) 69, 154, 193, 202, 217, 324, 341, 855, 922
ApeKI GCWGC 2 cut(s) 827, 1064
ArsI GACNNNNNNTTYG 4 cut(s) 526, 558, 600, 632
AsiGI ACCGGT 3 cut(s) 131, 670, 706
AspA2I CCTAGG 1 cut(s) 425
AspLEI GCGC 2 cut(s) 272, 743
AspS9I GGNCC 6 cut(s) 194, 429, 668, 697, 727, 799
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 159
AsuII TTCGAA 1 cut(s) 1109
AvaII GGWCC 5 cut(s) 429, 668, 697, 727, 799
AvrII CCTAGG 1 cut(s) 425
BarI GAAGNNNNNNTAC 2 cut(s) 1006, 1038
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 142
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 2 cut(s) 814, 1076
BccI CCATC 4 cut(s) 344, 389, 1037, 1091
BceAI ACGGC 5 cut(s) 83, 190, 208, 232, 339
BciT130I CCWGG 1 cut(s) 158
BciVI GTATCC 1 cut(s) 648
BcnI CCSGG 1 cut(s) 197
BfaI CTAG 2 cut(s) 26, 426
BfmI CTRYAG 2 cut(s) 57, 144
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 201
BglII AGATCT 1 cut(s) 1077
BisI GCNGC 4 cut(s) 220, 828, 922, 1065
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 4 cut(s) 221, 829, 923, 1066
Bme1390I CCNGG 2 cut(s) 158, 197
Bme18I GGWCC 5 cut(s) 429, 668, 697, 727, 799
BmgT120I GGNCC 6 cut(s) 194, 429, 668, 697, 727, 799
BmiI GGNNCC 3 cut(s) 310, 431, 669
BmrFI CCNGG 2 cut(s) 158, 197
BpiI GAAGAC 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 1 cut(s) 1101
Bpu10I CCTNAGC 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 1109
BpuEI CTTGAG 1 cut(s) 1044
BpuMI CCSGG 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 612
BsaJI CCNNGG 8 cut(s) 190, 250, 425, 717, 733, 778, 925, 955
BsaWI WCCGGW 3 cut(s) 131, 670, 706
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 706, 956
Bse118I RCCGGY 5 cut(s) 131, 204, 539, 670, 706
Bse1I ACTGG 2 cut(s) 802, 1118
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 8 cut(s) 190, 250, 425, 717, 733, 778, 925, 955
BseGI GGATG 3 cut(s) 336, 965, 1048
BseLI CCNNNNNNNGG 3 cut(s) 196, 706, 956
BseMII CTCAG 3 cut(s) 359, 474, 779
BseNI ACTGG 2 cut(s) 802, 1118
BseX3I CGGCCG 2 cut(s) 217, 324
BseXI GCAGC 2 cut(s) 814, 1076
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 3 cut(s) 220, 327, 675
BshFI GGCC 9 cut(s) 71, 156, 195, 204, 219, 326, 343, 857, 924
BshTI ACCGGT 3 cut(s) 131, 670, 706
BsiEI CGRYCG 3 cut(s) 220, 327, 675
BsiSI CCGG 8 cut(s) 132, 197, 205, 327, 340, 540, 671, 707
BslFI GGGAC 3 cut(s) 363, 415, 681
BslI CCNNNNNNNGG 3 cut(s) 196, 706, 956
BsmFI GGGAC 3 cut(s) 363, 415, 681
BsnI GGCC 9 cut(s) 71, 156, 195, 204, 219, 326, 343, 857, 924
Bsp119I TTCGAA 1 cut(s) 1109
Bsp1407I TGTACA 2 cut(s) 504, 625
Bsp143I GATC 4 cut(s) 329, 972, 1077, 1084
Bsp19I CCATGG 1 cut(s) 925
Bsp68I TCGCGA 1 cut(s) 320
BspACI CCGC 5 cut(s) 220, 700, 730, 921, 950
BspANI GGCC 9 cut(s) 71, 156, 195, 204, 219, 326, 343, 857, 924
BspCNI CTCAG 3 cut(s) 358, 475, 778
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 3 cut(s) 310, 431, 669
BspMAI CTGCAG 2 cut(s) 61, 148
BspPI GGATC 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 1109
BsrFI RCCGGY 5 cut(s) 131, 204, 539, 670, 706
BsrGI TGTACA 2 cut(s) 504, 625
BsrI ACTGG 2 cut(s) 802, 1118
BssAI RCCGGY 5 cut(s) 131, 204, 539, 670, 706
BssECI CCNNGG 8 cut(s) 190, 250, 425, 717, 733, 778, 925, 955
BssMI GATC 4 cut(s) 329, 972, 1077, 1084
BssNI GRCGYC 1 cut(s) 612
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 4 cut(s) 250, 425, 925, 955
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 4 cut(s) 54, 212, 632, 779
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 612
BstAUI TGTACA 2 cut(s) 504, 625
BstBI TTCGAA 1 cut(s) 1109
BstC8I GCNNGC 3 cut(s) 202, 792, 1138
BstDEI CTNAG 4 cut(s) 266, 345, 483, 765
BstDSI CCRYGG 4 cut(s) 190, 733, 778, 925
BstF5I GGATG 3 cut(s) 336, 965, 1048
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 2 cut(s) 272, 743
BstKTI GATC 4 cut(s) 332, 975, 1080, 1087
BstMBI GATC 4 cut(s) 329, 972, 1077, 1084
BstMCI CGRYCG 3 cut(s) 220, 327, 675
BstMWI GCNNNNNNNGC 3 cut(s) 201, 317, 738
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 2 cut(s) 559, 918
BstSCI CCNGG 2 cut(s) 156, 195
BstSFI CTRYAG 2 cut(s) 57, 144
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 814, 1076
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 1077
BstYI RGATCY 1 cut(s) 1077
BstZI CGGCCG 2 cut(s) 217, 324
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 9 cut(s) 71, 156, 195, 204, 219, 326, 343, 857, 924
BtgI CCRYGG 4 cut(s) 190, 733, 778, 925
BtsCI GGATG 3 cut(s) 336, 965, 1048
BtsIMutI CAGTG 1 cut(s) 50
BtuMI TCGCGA 1 cut(s) 320
Cac8I GCNNGC 3 cut(s) 202, 792, 1138
CfoI GCGC 2 cut(s) 272, 743
Cfr10I RCCGGY 5 cut(s) 131, 204, 539, 670, 706
Cfr13I GGNCC 6 cut(s) 194, 429, 668, 697, 727, 799
Csp6I GTAC 7 cut(s) 208, 400, 505, 593, 626, 937, 1049
CspAI ACCGGT 3 cut(s) 131, 670, 706
CviAII CATG 7 cut(s) 84, 556, 890, 915, 926, 983, 1011
CviQI GTAC 7 cut(s) 208, 400, 505, 593, 626, 937, 1049
DdeI CTNAG 4 cut(s) 266, 345, 483, 765
DpnI GATC 4 cut(s) 331, 974, 1079, 1086
DpnII GATC 4 cut(s) 329, 972, 1077, 1084
DraIII CACNNNGTG 1 cut(s) 796
EaeI YGGCCR 5 cut(s) 154, 202, 217, 324, 922
EagI CGGCCG 2 cut(s) 217, 324
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EclXI CGGCCG 2 cut(s) 217, 324
Eco130I CCWWGG 4 cut(s) 250, 425, 925, 955
Eco47I GGWCC 5 cut(s) 429, 668, 697, 727, 799
Eco52I CGGCCG 2 cut(s) 217, 324
EcoO109I RGGNCCY 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 4 cut(s) 250, 425, 925, 955
ErhI CCWWGG 4 cut(s) 250, 425, 925, 955
FaeI CATG 7 cut(s) 87, 559, 893, 918, 929, 986, 1014
FaqI GGGAC 3 cut(s) 363, 415, 681
FatI CATG 7 cut(s) 83, 555, 889, 914, 925, 982, 1010
FauNDI CATATG 1 cut(s) 838
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 220, 828, 922, 1065
FokI GGATG 3 cut(s) 323, 972, 1055
Fsp4HI GCNGC 4 cut(s) 220, 828, 922, 1065
FspBI CTAG 2 cut(s) 26, 426
GlaI GCGC 2 cut(s) 271, 742
GluI GCNGC 4 cut(s) 220, 828, 922, 1065
GsaI CCCAGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 9 cut(s) 71, 156, 195, 204, 219, 326, 343, 857, 924
HapII CCGG 8 cut(s) 132, 197, 205, 327, 340, 540, 671, 707
HhaI GCGC 2 cut(s) 272, 743
Hin1I GRCGYC 1 cut(s) 612
Hin1II CATG 7 cut(s) 87, 559, 893, 918, 929, 986, 1014
Hin6I GCGC 2 cut(s) 270, 741
HinP1I GCGC 2 cut(s) 270, 741
HinfI GANTC 5 cut(s) 5, 116, 179, 281, 445
HpaII CCGG 8 cut(s) 132, 197, 205, 327, 340, 540, 671, 707
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 6 cut(s) 167, 507, 628, 683, 727, 850
Hpy188I TCNGA 6 cut(s) 127, 348, 444, 525, 721, 1089
Hpy188III TCNNGA 5 cut(s) 278, 319, 550, 587, 652
Hpy8I GTNNAC 6 cut(s) 167, 507, 628, 683, 727, 850
Hpy99I CGWCG 3 cut(s) 325, 566, 617
HpyAV CCTTC 4 cut(s) 193, 520, 831, 1032
HpyCH4III ACNGT 4 cut(s) 54, 212, 632, 779
HpyCH4IV ACGT 3 cut(s) 163, 393, 612
HpyCH4V TGCA 4 cut(s) 59, 146, 464, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 317, 738
HpyF3I CTNAG 4 cut(s) 266, 345, 483, 765
HpySE526I ACGT 3 cut(s) 163, 393, 612
Hsp92I GRCGYC 1 cut(s) 612
Hsp92II CATG 7 cut(s) 87, 559, 893, 918, 929, 986, 1014
HspAI GCGC 2 cut(s) 270, 741
Kzo9I GATC 4 cut(s) 329, 972, 1077, 1084
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 2 cut(s) 814, 1076
MaeI CTAG 2 cut(s) 26, 426
MaeII ACGT 3 cut(s) 163, 393, 612
MaeIII GTNAC 3 cut(s) 48, 86, 212
MalI GATC 4 cut(s) 331, 974, 1079, 1086
MboI GATC 4 cut(s) 329, 972, 1077, 1084
MboII GAAGA 5 cut(s) 32, 142, 178, 413, 1072
MflI RGATCY 1 cut(s) 1077
MluCI AATT 3 cut(s) 79, 940, 1036
MlyI GAGTC 3 cut(s) 110, 188, 275
MmeI TCCRAC 1 cut(s) 150
MseI TTAA 6 cut(s) 389, 573, 785, 806, 897, 1153
MspI CCGG 8 cut(s) 132, 197, 205, 327, 340, 540, 671, 707
MspR9I CCNGG 2 cut(s) 158, 197
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 201, 317, 738
NciI CCSGG 1 cut(s) 197
NcoI CCATGG 1 cut(s) 925
NdeI CATATG 1 cut(s) 838
NdeII GATC 4 cut(s) 329, 972, 1077, 1084
NlaIII CATG 7 cut(s) 87, 559, 893, 918, 929, 986, 1014
NlaIV GGNNCC 3 cut(s) 310, 431, 669
NmuCI GTSAC 2 cut(s) 48, 212
NruI TCGCGA 1 cut(s) 320
NspI RCATGY 2 cut(s) 559, 918
NspV TTCGAA 1 cut(s) 1109
PciI ACATGT 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 329
PfeI GAWTC 2 cut(s) 5, 445
PflFI GACNNNGTC 1 cut(s) 614
PinAI ACCGGT 3 cut(s) 131, 670, 706
PkrI GCNGC 4 cut(s) 221, 829, 923, 1066
PleI GAGTC 3 cut(s) 110, 187, 275
PpsI GAGTC 3 cut(s) 110, 187, 275
PpuMI RGGWCCY 1 cut(s) 429
PscI ACATGT 1 cut(s) 555
Psp1406I AACGTT 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 156
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 3 cut(s) 310, 431, 669
PspPI GGNCC 6 cut(s) 194, 429, 668, 697, 727, 799
PspPPI RGGWCCY 1 cut(s) 429
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PstI CTGCAG 2 cut(s) 61, 148
PsuI RGATCY 1 cut(s) 1077
PsyI GACNNNGTC 1 cut(s) 614
RruI TCGCGA 1 cut(s) 320
RsaI GTAC 7 cut(s) 209, 401, 506, 594, 627, 938, 1050
RsaNI GTAC 7 cut(s) 208, 400, 505, 593, 626, 937, 1049
SaqAI TTAA 6 cut(s) 389, 573, 785, 806, 897, 1153
SatI GCNGC 4 cut(s) 220, 828, 922, 1065
Sau3AI GATC 4 cut(s) 329, 972, 1077, 1084
Sau96I GGNCC 6 cut(s) 194, 429, 668, 697, 727, 799
SchI GAGTC 3 cut(s) 110, 188, 275
ScrFI CCNGG 2 cut(s) 158, 197
SfcI CTRYAG 2 cut(s) 57, 144
SfiI GGCCNNNNNGGCC 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 1109
SgrAI CRCCGGYG 1 cut(s) 131
SinI GGWCC 5 cut(s) 429, 668, 697, 727, 799
SmlI CTYRAG 1 cut(s) 1059
SmoI CTYRAG 1 cut(s) 1059
Sse9I AATT 3 cut(s) 79, 940, 1036
SsiI CCGC 5 cut(s) 220, 700, 730, 921, 950
SspI AATATT 1 cut(s) 996
SspMI CTAG 2 cut(s) 26, 426
StyD4I CCNGG 2 cut(s) 156, 195
StyI CCWWGG 4 cut(s) 250, 425, 925, 955
TaaI ACNGT 4 cut(s) 54, 212, 632, 779
TaiI ACGT 3 cut(s) 166, 396, 615
TaqI TCGA 7 cut(s) 279, 332, 448, 513, 597, 1083, 1109
TasI AATT 3 cut(s) 79, 940, 1036
TatI WGTACW 5 cut(s) 399, 504, 592, 625, 936
TauI GCSGC 2 cut(s) 222, 924
TfiI GAWTC 2 cut(s) 5, 445
Tru1I TTAA 6 cut(s) 389, 573, 785, 806, 897, 1153
Tru9I TTAA 6 cut(s) 389, 573, 785, 806, 897, 1153
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 2 cut(s) 48, 212
TseI GCWGC 2 cut(s) 827, 1064
Tsp45I GTSAC 2 cut(s) 48, 212
TspDTI ATGAA 4 cut(s) 72, 771, 971, 1092
TspGWI ACGGA 2 cut(s) 321, 550
TspRI CASTG 1 cut(s) 57
Tth111I GACNNNGTC 1 cut(s) 614
VpaK11BI GGWCC 5 cut(s) 429, 668, 697, 727, 799
XceI RCATGY 2 cut(s) 559, 918
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmaJI CCTAGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 2 cut(s) 26, 426
ZraI GACGTC 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.