MD16G1099800.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003753
Physical Location & Seq
Forward (+)
6937263 .. 6939143
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1099800.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGACCGTGCGAGGTTTATTTCTATCGGCAGGTGGTGGAGAGGACCATGGGGACTGAAGAAGAGTGTGGAAGCAGTGGCAGTTGGAGTTGTTTTAGTTGCTGCTCTTTCAACTTCCTTCGGCCTTGTACCACAGAAGAAATCGCGCAGTGCTTGTCAATTCCCGGCAATGTATAACTTTGGGGATTCAAATTCGGACACTGGTAGTTTCTCAGCGACATTTTATCGGCTTCCTTCTCCCTATGGCTATACCTTCTTTGGTAAACCTTCTGGCAGGTTCTCAGACGGGCGTATCATCATCGATTTTATAGCTCAAAAGTTGGGGTTTCCATTTTTAAGTGCTTATCTGAATTCTATTGGAGCAAACTTCCGTTGTGGAGCGAATTTCGCGATTGTAGCATCTACGATTCAGCCACTAGATGTCAGGACGTTTGGAGATGGATATAGCCCCATCTCTCTTAACGTACAGCTTTCGCAATTTGCGCAGTTCAAAGCACGTGTAAATGAGTTTTTCCCTCGAGATGAAAGGTCATACGTCAAGGCCGGCCTCCCAAGAGCAGAGGACTTCTCCAAGGCCTTGTACACTTTAGACATTGGACAAAACGATCTTAGTGCCGGGTTAAGCTGGAAGACGGCAGACCAGCTGCTGGAAAACGTTTCCAGCATAACAGCGCAGTTAGCCTTGACAATCGAGCAACTCTACCAACAAGGGGCAAGAGTTTTTTGGATTCACAACACAGGTCCGCTCGGGTGCTTGCCTTCAACTCTGGCGTATAAGATGGCAGAACCCGGCGACCTGGATCAAAACGGTTGCTTGAAGCGGTACAATGAAGTGTGTCAAGAATTTAACAGGCAGCTCAAGGAGAGAGTGCTTAAACTAAGAGCCAAACTTTCAGAAGCAGTTTTTACTTACATCGATGTCTATGCAGCAAAATACACACTAATCAGTGAAGCGGAGAAGTATGGTTTTACGAGTCCTCTAGCTCAATGCTGTGGAAGCTACGGTGATGTCCCTGTCCGTTGTGGGGTGAAAGCTATTGTTAATGGCACTGAAGTTGGAGGTCCTTGTAGCAATCCTTCTCAGCACATTAACTGGGATGGAGGACACTATTCTGATGCTGCGAACGAGTGGCTAGCCACCCGCATTATGGACGGCGCATTCTCTGATCCTCCGGTTTCAATCACTGAAGCATGTCACAAAACTCCACGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

405

Amino Acids

44.54

Weight (kDa)

8.57

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 382 5e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 21
Acc16I TGCGCA 1 cut(s) 483
Acc36I ACCTGC 2 cut(s) 21, 264
AccB7I CCANNNNNTGG 1 cut(s) 646
AccBSI CCGCTC 1 cut(s) 745
AccII CGCG 2 cut(s) 145, 389
AciI CCGC 4 cut(s) 743, 820, 953, 1141
AclI AACGTT 1 cut(s) 654
AclWI GGATC 2 cut(s) 807, 1160
AcsI RAATTY 4 cut(s) 190, 349, 382, 842
AcuI CTGAAG 3 cut(s) 77, 1071, 1206
AcvI CACGTG 1 cut(s) 497
AfaI GTAC 4 cut(s) 129, 465, 581, 824
AfiI CCNNNNNNNGG 4 cut(s) 646, 709, 1024, 1147
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 6 cut(s) 111, 189, 490, 762, 817, 1179
AjnI CCWGG 1 cut(s) 795
AluBI AGCT 8 cut(s) 311, 469, 624, 643, 856, 983, 999, 1034
AluI AGCT 8 cut(s) 311, 469, 624, 643, 856, 983, 999, 1034
AlwI GGATC 2 cut(s) 807, 1160
AlwNI CAGNNNCTG 1 cut(s) 646
Ama87I CYCGRG 2 cut(s) 516, 746
AoxI GGCC 4 cut(s) 121, 540, 544, 573
ApeKI GCWGC 5 cut(s) 101, 643, 853, 926, 1118
ApoI RAATTY 4 cut(s) 190, 349, 382, 842
AspLEI GCGC 4 cut(s) 147, 484, 673, 1157
AspS9I GGNCC 4 cut(s) 4, 44, 740, 1061
AsuC2I CCSGG 3 cut(s) 164, 616, 789
AsuHPI GGTGA 2 cut(s) 1016, 1039
AsuNHI GCTAGC 1 cut(s) 1132
AvaI CYCGRG 2 cut(s) 516, 746
AvaII GGWCC 4 cut(s) 4, 44, 740, 1061
BaeI ACNNNNGTAYC 2 cut(s) 274, 307
BarI GAAGNNNNNNTAC 2 cut(s) 1060, 1092
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 635
BbvI GCAGC 5 cut(s) 88, 630, 865, 938, 1105
BccI CCATC 4 cut(s) 431, 458, 772, 1091
BceAI ACGGC 2 cut(s) 648, 1168
BcgI CGANNNNNNTGC 4 cut(s) 593, 627, 905, 939
BciT130I CCWGG 1 cut(s) 797
BcnI CCSGG 3 cut(s) 164, 616, 789
BfaI CTAG 3 cut(s) 416, 980, 1133
BfuAI ACCTGC 2 cut(s) 21, 264
BisI GCNGC 5 cut(s) 102, 644, 854, 927, 1119
BlsI GCNGC 5 cut(s) 103, 645, 855, 928, 1120
Bme1390I CCNGG 4 cut(s) 164, 616, 789, 797
Bme18I GGWCC 4 cut(s) 4, 44, 740, 1061
BmeT110I CYCGRG 2 cut(s) 516, 746
BmgT120I GGNCC 4 cut(s) 4, 44, 740, 1061
BmrFI CCNGG 4 cut(s) 164, 616, 789, 797
BmrI ACTGGG 1 cut(s) 1102
BmsI GCATC 2 cut(s) 407, 1105
BmtI GCTAGC 1 cut(s) 1136
BmuI ACTGGG 1 cut(s) 1102
BpiI GAAGAC 1 cut(s) 635
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpuEI CTTGAG 1 cut(s) 842
BpuMI CCSGG 3 cut(s) 164, 616, 789
Bsa29I ATCGAT 2 cut(s) 300, 915
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 47, 570, 1205
BsaWI WCCGGW 1 cut(s) 1171
Bsc4I CCNNNNNNNGG 4 cut(s) 646, 709, 1024, 1147
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 2 cut(s) 205, 1097
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 797
BseCI ATCGAT 2 cut(s) 300, 915
BseDI CCNNGG 3 cut(s) 47, 570, 1205
BseGI GGATG 1 cut(s) 1102
BseLI CCNNNNNNNGG 4 cut(s) 646, 709, 1024, 1147
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 3 cut(s) 225, 294, 1094
BseNI ACTGG 2 cut(s) 205, 1097
BseXI GCAGC 5 cut(s) 88, 630, 865, 938, 1105
Bsh1236I CGCG 2 cut(s) 145, 389
BshFI GGCC 4 cut(s) 123, 542, 546, 575
BshVI ATCGAT 2 cut(s) 300, 915
BsiHKCI CYCGRG 2 cut(s) 516, 746
BsiSI CCGG 5 cut(s) 164, 543, 615, 789, 1172
BslFI GGGAC 2 cut(s) 66, 995
BslI CCNNNNNNNGG 4 cut(s) 646, 709, 1024, 1147
BsmFI GGGAC 2 cut(s) 66, 995
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 4 cut(s) 123, 542, 546, 575
BsoBI CYCGRG 2 cut(s) 516, 746
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 3 cut(s) 604, 799, 1165
Bsp19I CCATGG 1 cut(s) 47
Bsp68I TCGCGA 1 cut(s) 389
BspACI CCGC 4 cut(s) 743, 820, 953, 1141
BspANI GGCC 4 cut(s) 123, 542, 546, 575
BspCNI CTCAG 3 cut(s) 224, 293, 1093
BspDI ATCGAT 2 cut(s) 300, 915
BspFNI CGCG 2 cut(s) 145, 389
BspMI ACCTGC 2 cut(s) 21, 264
BspOI GCTAGC 1 cut(s) 1136
BspPI GGATC 2 cut(s) 807, 1160
BsrBI CCGCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 2 cut(s) 205, 1097
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 47, 570, 1205
BssMI GATC 3 cut(s) 604, 799, 1165
BssT1I CCWWGG 2 cut(s) 47, 570
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 4 cut(s) 8, 809, 1004, 1209
Bst6I CTCTTC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 3 cut(s) 544, 755, 1134
BstDEI CTNAG 5 cut(s) 211, 280, 608, 878, 1080
BstDSI CCRYGG 2 cut(s) 47, 1205
BstF5I GGATG 1 cut(s) 1102
BstFNI CGCG 2 cut(s) 145, 389
BstHHI GCGC 4 cut(s) 147, 484, 673, 1157
BstKTI GATC 3 cut(s) 607, 802, 1168
BstMBI GATC 3 cut(s) 604, 799, 1165
BstMWI GCNNNNNNNGC 5 cut(s) 386, 395, 481, 677, 996
BstNI CCWGG 1 cut(s) 797
BstNSI RCATGY 1 cut(s) 1194
BstSCI CCNGG 4 cut(s) 162, 614, 787, 795
BstUI CGCG 2 cut(s) 145, 389
BstV1I GCAGC 5 cut(s) 88, 630, 865, 938, 1105
BstV2I GAAGAC 1 cut(s) 635
Bsu15I ATCGAT 2 cut(s) 300, 915
BsuRI GGCC 4 cut(s) 123, 542, 546, 575
BsuTUI ATCGAT 2 cut(s) 300, 915
BtgI CCRYGG 2 cut(s) 47, 1205
BtsCI GGATG 1 cut(s) 1102
BtsI GCAGTG 2 cut(s) 81, 154
BtsIMutI CAGTG 6 cut(s) 81, 154, 198, 952, 1047, 1182
BtuMI TCGCGA 1 cut(s) 389
BveI ACCTGC 2 cut(s) 21, 264
Cac8I GCNNGC 3 cut(s) 544, 755, 1134
CaiI CAGNNNCTG 1 cut(s) 646
CfoI GCGC 4 cut(s) 147, 484, 673, 1157
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 4 cut(s) 4, 44, 740, 1061
ClaI ATCGAT 2 cut(s) 300, 915
Csp6I GTAC 4 cut(s) 128, 464, 580, 823
CviAII CATG 2 cut(s) 48, 1191
CviQI GTAC 4 cut(s) 128, 464, 580, 823
DdeI CTNAG 5 cut(s) 211, 280, 608, 878, 1080
DpnI GATC 3 cut(s) 606, 801, 1167
DpnII GATC 3 cut(s) 604, 799, 1165
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 47, 570
Eco147I AGGCCT 1 cut(s) 575
Eco47I GGWCC 4 cut(s) 4, 44, 740, 1061
Eco57I CTGAAG 3 cut(s) 77, 1071, 1206
Eco72I CACGTG 1 cut(s) 497
Eco88I CYCGRG 2 cut(s) 516, 746
EcoO109I RGGNCCY 1 cut(s) 1061
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 2 cut(s) 47, 570
ErhI CCWWGG 2 cut(s) 47, 570
FaeI CATG 2 cut(s) 51, 1194
FaqI GGGAC 2 cut(s) 66, 995
FatI CATG 2 cut(s) 47, 1190
FauI CCCGC 1 cut(s) 1148
Fnu4HI GCNGC 5 cut(s) 102, 644, 854, 927, 1119
FokI GGATG 1 cut(s) 1109
FseI GGCCGGCC 1 cut(s) 546
Fsp4HI GCNGC 5 cut(s) 102, 644, 854, 927, 1119
FspBI CTAG 3 cut(s) 416, 980, 1133
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 4 cut(s) 146, 483, 672, 1156
GluI GCNGC 5 cut(s) 102, 644, 854, 927, 1119
HaeIII GGCC 4 cut(s) 123, 542, 546, 575
HapII CCGG 5 cut(s) 164, 543, 615, 789, 1172
HhaI GCGC 4 cut(s) 147, 484, 673, 1157
Hin1II CATG 2 cut(s) 51, 1194
Hin6I GCGC 4 cut(s) 145, 482, 671, 1155
HinP1I GCGC 4 cut(s) 145, 482, 671, 1155
HinfI GANTC 4 cut(s) 185, 406, 727, 973
HpaII CCGG 5 cut(s) 164, 543, 615, 789, 1172
HphI GGTGA 2 cut(s) 1016, 1039
Hpy166II GTNNAC 2 cut(s) 263, 582
Hpy188I TCNGA 6 cut(s) 196, 283, 348, 895, 1114, 1165
Hpy188III TCNNGA 4 cut(s) 388, 424, 518, 839
Hpy8I GTNNAC 2 cut(s) 263, 582
HpyAV CCTTC 6 cut(s) 127, 243, 262, 276, 768, 1086
HpyCH4III ACNGT 4 cut(s) 8, 809, 1004, 1209
HpyCH4IV ACGT 5 cut(s) 428, 462, 496, 534, 654
HpyCH4V TGCA 1 cut(s) 926
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 395, 481, 677, 996
HpyF3I CTNAG 5 cut(s) 211, 280, 608, 878, 1080
HpySE526I ACGT 5 cut(s) 428, 462, 496, 534, 654
Hsp92II CATG 2 cut(s) 51, 1194
HspAI GCGC 4 cut(s) 145, 482, 671, 1155
KroI GCCGGC 1 cut(s) 542
KroNI GCCGGC 1 cut(s) 544
Kzo9I GATC 3 cut(s) 604, 799, 1165
LmnI GCTCC 2 cut(s) 359, 377
Lsp1109I GCAGC 5 cut(s) 88, 630, 865, 938, 1105
LweI GCATC 2 cut(s) 407, 1105
MaeI CTAG 3 cut(s) 416, 980, 1133
MaeII ACGT 5 cut(s) 428, 462, 496, 534, 654
MaeIII GTNAC 1 cut(s) 1193
MalI GATC 3 cut(s) 606, 801, 1167
MbiI CCGCTC 1 cut(s) 745
MboI GATC 3 cut(s) 604, 799, 1165
MboII GAAGA 4 cut(s) 70, 73, 148, 640
MluCI AATT 6 cut(s) 158, 190, 349, 382, 476, 842
MlyI GAGTC 1 cut(s) 982
MmeI TCCRAC 2 cut(s) 64, 1036
MnlI CCTC 9 cut(s) 6, 35, 525, 553, 557, 987, 1052, 1094, 1179
MroNI GCCGGC 1 cut(s) 542
MseI TTAA 7 cut(s) 335, 459, 620, 846, 873, 1041, 1089
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 5 cut(s) 164, 543, 615, 789, 1172
MspR9I CCNGG 4 cut(s) 164, 616, 789, 797
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 2 cut(s) 145, 389
MwoI GCNNNNNNNGC 5 cut(s) 386, 395, 481, 677, 996
NaeI GCCGGC 1 cut(s) 544
NciI CCSGG 3 cut(s) 164, 616, 789
NcoI CCATGG 1 cut(s) 47
NdeII GATC 3 cut(s) 604, 799, 1165
NgoMIV GCCGGC 1 cut(s) 542
NheI GCTAGC 1 cut(s) 1132
NlaIII CATG 2 cut(s) 51, 1194
NmuCI GTSAC 1 cut(s) 1193
NruI TCGCGA 1 cut(s) 389
NsbI TGCGCA 1 cut(s) 483
NspI RCATGY 1 cut(s) 1194
PaeR7I CTCGAG 1 cut(s) 516
PaqCI CACCTGC 1 cut(s) 21
PceI AGGCCT 1 cut(s) 575
PctI GAATGC 1 cut(s) 1157
PdiI GCCGGC 1 cut(s) 544
PfeI GAWTC 3 cut(s) 185, 406, 727
PflMI CCANNNNNTGG 1 cut(s) 646
PkrI GCNGC 5 cut(s) 103, 645, 855, 928, 1120
PleI GAGTC 1 cut(s) 981
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 981
Ppu21I YACGTR 1 cut(s) 497
PpuMI RGGWCCY 1 cut(s) 1061
Psp1406I AACGTT 1 cut(s) 654
Psp5II RGGWCCY 1 cut(s) 1061
Psp6I CCWGG 1 cut(s) 795
PspCI CACGTG 1 cut(s) 497
PspGI CCWGG 1 cut(s) 795
PspPI GGNCC 4 cut(s) 4, 44, 740, 1061
PspPPI RGGWCCY 1 cut(s) 1061
PstNI CAGNNNCTG 1 cut(s) 646
PvuII CAGCTG 1 cut(s) 643
RigI GGCCGGCC 1 cut(s) 546
RruI TCGCGA 1 cut(s) 389
RsaI GTAC 4 cut(s) 129, 465, 581, 824
RsaNI GTAC 4 cut(s) 128, 464, 580, 823
SaqAI TTAA 7 cut(s) 335, 459, 620, 846, 873, 1041, 1089
SatI GCNGC 5 cut(s) 102, 644, 854, 927, 1119
Sau3AI GATC 3 cut(s) 604, 799, 1165
Sau96I GGNCC 4 cut(s) 4, 44, 740, 1061
SchI GAGTC 1 cut(s) 982
ScrFI CCNGG 4 cut(s) 164, 616, 789, 797
SfaNI GCATC 2 cut(s) 407, 1105
Sfr274I CTCGAG 1 cut(s) 516
SinI GGWCC 4 cut(s) 4, 44, 740, 1061
SlaI CTCGAG 1 cut(s) 516
SmlI CTYRAG 2 cut(s) 516, 857
SmoI CTYRAG 2 cut(s) 516, 857
Sse9I AATT 6 cut(s) 158, 190, 349, 382, 476, 842
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 4 cut(s) 743, 820, 953, 1141
SspMI CTAG 3 cut(s) 416, 980, 1133
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 4 cut(s) 162, 614, 787, 795
StyI CCWWGG 2 cut(s) 47, 570
TaaI ACNGT 4 cut(s) 8, 809, 1004, 1209
TaiI ACGT 5 cut(s) 431, 465, 499, 537, 657
TaqI TCGA 4 cut(s) 300, 517, 690, 915
TasI AATT 6 cut(s) 158, 190, 349, 382, 476, 842
TatI WGTACW 1 cut(s) 579
TfiI GAWTC 3 cut(s) 185, 406, 727
Tru1I TTAA 7 cut(s) 335, 459, 620, 846, 873, 1041, 1089
Tru9I TTAA 7 cut(s) 335, 459, 620, 846, 873, 1041, 1089
TscAI CASTG 6 cut(s) 81, 154, 205, 952, 1054, 1189
TseFI GTSAC 1 cut(s) 1193
TseI GCWGC 5 cut(s) 101, 643, 853, 926, 1118
Tsp45I GTSAC 1 cut(s) 1193
TspDTI ATGAA 2 cut(s) 537, 843
TspGWI ACGGA 2 cut(s) 359, 1007
TspRI CASTG 6 cut(s) 81, 154, 205, 952, 1054, 1189
Van91I CCANNNNNTGG 1 cut(s) 646
VpaK11BI GGWCC 4 cut(s) 4, 44, 740, 1061
XapI RAATTY 4 cut(s) 190, 349, 382, 842
XceI RCATGY 1 cut(s) 1194
XcmI CCANNNNNNNNNTGG 1 cut(s) 1144
XhoI CTCGAG 1 cut(s) 516
XspI CTAG 3 cut(s) 416, 980, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.