Rh4BG331400

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003753
Physical Location & Seq
Forward (+)
49619489 .. 49621494
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG331400.1

Sequence Viewer

Length: 1194 bp
ATGGACCCTGTGAGAGGGCACTGGGGACTGAAGAGGAGTGTGGAAGCAGTAGCAGTTGGAGTTGTTTTAGTTGGTGTACTTTCAGTTTTTCTGGGCCTCAGACCCCAAAAGATTCAATCAAGTCGTTGTCATTTTCCGGCAATCTATAACTTTGGCGATTCAAATTCCGACACAGGTGCTTCATCAGCTGTATTTTATCGGCTTCCTTCTCCCTATGGCAATACATTCTTTGGCAAACCTTCGGGAAGGTTATCCGATGGGCGTCTCATGATCGATTTTGTAGCTCAGAAGTTGGGTTTGCCATTTTTGAGTGCTTATCTTGATTCTGTTGGAGCAAACTTCCGTCATGGAGCAAATTTTGCAACGGGAGGATCTACAATCTTGCCAATAGACGGAAAATTGTTCGAAGTAAGATTTAGCCCCATCTCTCTTGGCAAACAGTTTTCGCAATTTGCACAGCTTAAAGCTCGTGTAAATGAGGTTCTAAGTCAAGATAAAAGCTCATATGTCAGCGCCAGCATCCCAAGACCAGAGGACTTTTCAAAGGCATTGTACACCTTCGACATGGGACAAAACGATATTTATGCGGGATTAAGGTTTAAAACGGTGGACCAAGTGCTGGAATCCATTTCCGACATGACAGCTCAGTTAGCTTTGACAATAGAGCAACTATACCAGCAAGGCGGAAGAATATTTTGGATCCATAACACAGGTCCACTCGGCTGCTTGCCTATGTGTCTTGCACATAATCAGCCAGAACCCGATGACCTGGACCAGAATGGCTGTTTGAAGTCTTACAATGAGGTGGCCCAGGAGTTTAACAGACAGCTCAAGGAAAGAGTGCTTAATCTAAGAGCCAAGCTCTCGGATGCAGTTCTTACATATGTTGATCTCTATACAGCAAAATACACGCTTATTAGTGAAACAGAGAAGCATGGTTTTAGTGATCCTCTAGCTCAATGCTGCGGACGCCAAGGTGATTCTCCTTTCCATTGCGGGATGAATACTACTGCTGTCAATGGCACTGAAGTATTTGGTGGCTCTTGTAGTAATCCTGCAAAGTACATTAGCTGGGACGGTGTGCACTATTCTGATGCTGCAAACGAATGGGTAGCCAAACGAGTTATAGACGGCTCATTCTCAGACCCTCCAATTTCGATCACTGAAGCATGTCGCAAGCCTTCACAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

397

Amino Acids

43.58

Weight (kDa)

6.45

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 48 - 375 1.1e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 619
AciI CCGC 4 cut(s) 587, 684, 966, 996
AclWI GGATC 4 cut(s) 379, 694, 707, 941
AcsI RAATTY 2 cut(s) 163, 355
AcuI CTGAAG 3 cut(s) 50, 1047, 1185
AcyI GRCGYC 2 cut(s) 262, 970
AfaI GTAC 3 cut(s) 78, 554, 1064
AfiI CCNNNNNNNGG 3 cut(s) 14, 392, 619
AgsI TTSAA 4 cut(s) 116, 162, 543, 790
AjnI CCWGG 2 cut(s) 768, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
Alw21I GWGCWC 1 cut(s) 1086
Alw26I GTCTC 1 cut(s) 269
Alw44I GTGCAC 1 cut(s) 1082
AlwI GGATC 4 cut(s) 379, 694, 707, 941
AoxI GGCC 2 cut(s) 94, 807
ApaLI GTGCAC 1 cut(s) 1082
ApeKI GCWGC 3 cut(s) 723, 963, 1097
ApoI RAATTY 2 cut(s) 163, 355
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 6 cut(s) 4, 94, 610, 713, 772, 808
AsuHPI GGTGA 1 cut(s) 989
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 4 cut(s) 4, 610, 713, 772
BaeGI GKGCMC 2 cut(s) 21, 1086
BamHI GGATCC 1 cut(s) 699
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 1086
BbvI GCAGC 3 cut(s) 710, 950, 1084
BccI CCATC 2 cut(s) 251, 431
BceAI ACGGC 1 cut(s) 1147
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 566, 600
BciT130I CCWGG 2 cut(s) 770, 812
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 1 cut(s) 953
BfoI RGCGCY 1 cut(s) 516
BisI GCNGC 3 cut(s) 724, 964, 1098
BlsI GCNGC 3 cut(s) 725, 965, 1099
Bme1390I CCNGG 2 cut(s) 770, 812
Bme18I GGWCC 4 cut(s) 4, 610, 713, 772
BmgT120I GGNCC 6 cut(s) 4, 94, 610, 713, 772, 808
BmiI GGNNCC 2 cut(s) 6, 701
BmrFI CCNGG 2 cut(s) 770, 812
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 3 cut(s) 528, 859, 1084
BmuI ACTGGG 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 846, 878
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 815
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 262, 970
BsaJI CCNNGG 2 cut(s) 810, 973
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 392, 619
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 991
BseBI CCWGG 2 cut(s) 770, 812
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 810, 973
BseGI GGATG 3 cut(s) 519, 874, 1005
BseLI CCNNNNNNNGG 3 cut(s) 14, 392, 619
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 4 cut(s) 112, 299, 659, 1155
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 2 cut(s) 21, 1086
BseXI GCAGC 3 cut(s) 710, 950, 1084
BseYI CCCAGC 1 cut(s) 1071
BshFI GGCC 2 cut(s) 96, 809
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 1086
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 3 cut(s) 39, 582, 1088
BslI CCNNNNNNNGG 3 cut(s) 14, 392, 619
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 3 cut(s) 39, 582, 1088
BsnI GGCC 2 cut(s) 96, 809
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 21, 1086
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
BspACI CCGC 4 cut(s) 587, 684, 966, 996
BspANI GGCC 2 cut(s) 96, 809
BspCNI CTCAG 4 cut(s) 111, 298, 658, 1154
BspDI ATCGAT 1 cut(s) 273
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 6, 701
BspPI GGATC 4 cut(s) 379, 694, 707, 941
BspT104I TTCGAA 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 991
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 810, 973
BssMI GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
BssNI GRCGYC 2 cut(s) 262, 970
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 973
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 2 cut(s) 770, 812
Bst4CI ACNGT 3 cut(s) 441, 607, 1079
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 2 cut(s) 262, 970
BstAPI GCANNNNNTGC 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 517, 728, 1178
BstDEI CTNAG 6 cut(s) 98, 285, 485, 645, 851, 1141
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 519, 874, 1005
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 6 cut(s) 273, 374, 702, 892, 949, 1161
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
BstMWI GCNNNNNNNGC 5 cut(s) 185, 359, 650, 969, 1186
BstNI CCWGG 2 cut(s) 770, 812
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 2 cut(s) 768, 810
BstSLI GKGCMC 2 cut(s) 21, 1086
BstV1I GCAGC 3 cut(s) 710, 950, 1084
BstX2I RGATCY 2 cut(s) 371, 699
BstYI RGATCY 2 cut(s) 371, 699
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 96, 809
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 3 cut(s) 519, 874, 1005
BtsIMutI CAGTG 3 cut(s) 19, 1023, 1161
Cac8I GCNNGC 3 cut(s) 517, 728, 1178
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 6 cut(s) 4, 94, 610, 713, 772, 808
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 2 cut(s) 251, 978
Csp6I GTAC 3 cut(s) 77, 553, 1063
CviAII CATG 6 cut(s) 268, 347, 565, 637, 935, 1170
CviQI GTAC 3 cut(s) 77, 553, 1063
DdeI CTNAG 6 cut(s) 98, 285, 485, 645, 851, 1141
DpnI GATC 6 cut(s) 272, 373, 701, 891, 948, 1160
DpnII GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
DraI TTTAAA 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 973
Eco47I GGWCC 4 cut(s) 4, 610, 713, 772
Eco57I CTGAAG 3 cut(s) 50, 1047, 1185
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 768, 810
EcoT14I CCWWGG 1 cut(s) 973
ErhI CCWWGG 1 cut(s) 973
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 6 cut(s) 271, 350, 568, 640, 938, 1173
FaqI GGGAC 3 cut(s) 39, 582, 1088
FatI CATG 6 cut(s) 267, 346, 564, 636, 934, 1169
FauI CCCGC 2 cut(s) 580, 989
FauNDI CATATG 2 cut(s) 505, 883
Fnu4HI GCNGC 3 cut(s) 724, 964, 1098
FokI GGATG 3 cut(s) 506, 881, 1012
Fsp4HI GCNGC 3 cut(s) 724, 964, 1098
FspBI CTAG 1 cut(s) 953
GlaI GCGC 1 cut(s) 514
GluI GCNGC 3 cut(s) 724, 964, 1098
GsaI CCCAGC 1 cut(s) 1075
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 2 cut(s) 96, 809
HapII CCGG 1 cut(s) 137
HgaI GACGC 2 cut(s) 251, 978
HhaI GCGC 1 cut(s) 515
Hin1I GRCGYC 2 cut(s) 262, 970
Hin1II CATG 6 cut(s) 271, 350, 568, 640, 938, 1173
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HinfI GANTC 5 cut(s) 112, 158, 323, 623, 980
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 989
Hpy166II GTNNAC 5 cut(s) 77, 555, 610, 716, 1084
Hpy188I TCNGA 8 cut(s) 101, 169, 256, 288, 634, 868, 1093, 1144
Hpy188III TCNNGA 4 cut(s) 243, 268, 320, 491
Hpy8I GTNNAC 5 cut(s) 77, 555, 610, 716, 1084
HpyAV CCTTC 5 cut(s) 216, 240, 249, 568, 1191
HpyCH4III ACNGT 3 cut(s) 441, 607, 1079
HpyCH4V TGCA 7 cut(s) 362, 455, 743, 872, 1058, 1084, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 359, 650, 969, 1186
HpyF3I CTNAG 6 cut(s) 98, 285, 485, 645, 851, 1141
Hsp92I GRCGYC 2 cut(s) 262, 970
Hsp92II CATG 6 cut(s) 271, 350, 568, 640, 938, 1173
HspAI GCGC 1 cut(s) 513
Kzo9I GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
LmnI GCTCC 2 cut(s) 332, 350
Lsp1109I GCAGC 3 cut(s) 710, 950, 1084
LweI GCATC 3 cut(s) 528, 859, 1084
MaeI CTAG 1 cut(s) 953
MalI GATC 6 cut(s) 272, 373, 701, 891, 948, 1160
MboI GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
MboII GAAGA 2 cut(s) 43, 699
MflI RGATCY 2 cut(s) 371, 699
MhlI GDGCHC 2 cut(s) 21, 1086
MluCI AATT 5 cut(s) 163, 355, 398, 449, 1152
MmeI TCCRAC 4 cut(s) 37, 192, 310, 657
MnlI CCTC 9 cut(s) 8, 27, 107, 362, 472, 526, 796, 960, 1158
MseI TTAA 5 cut(s) 462, 593, 600, 819, 846
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 770, 812
MvaI CCWGG 2 cut(s) 770, 812
MwoI GCNNNNNNNGC 5 cut(s) 185, 359, 650, 969, 1186
NdeI CATATG 2 cut(s) 505, 883
NdeII GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
NlaIII CATG 6 cut(s) 271, 350, 568, 640, 938, 1173
NlaIV GGNNCC 2 cut(s) 6, 701
NmeAIII GCCGAG 1 cut(s) 699
NspI RCATGY 1 cut(s) 1173
NspV TTCGAA 1 cut(s) 405
PagI TCATGA 1 cut(s) 267
PfeI GAWTC 5 cut(s) 112, 158, 323, 623, 980
PflMI CCANNNNNTGG 1 cut(s) 619
PkrI GCNGC 3 cut(s) 725, 965, 1099
Psp6I CCWGG 2 cut(s) 768, 810
PspFI CCCAGC 1 cut(s) 1071
PspGI CCWGG 2 cut(s) 768, 810
PspN4I GGNNCC 2 cut(s) 6, 701
PspPI GGNCC 6 cut(s) 4, 94, 610, 713, 772, 808
PsrI GAACNNNNNNTAC 2 cut(s) 465, 497
PsuI RGATCY 2 cut(s) 371, 699
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 3 cut(s) 78, 554, 1064
RsaNI GTAC 3 cut(s) 77, 553, 1063
SaqAI TTAA 5 cut(s) 462, 593, 600, 819, 846
SatI GCNGC 3 cut(s) 724, 964, 1098
Sau3AI GATC 6 cut(s) 270, 371, 699, 889, 946, 1158
Sau96I GGNCC 6 cut(s) 4, 94, 610, 713, 772, 808
ScrFI CCNGG 2 cut(s) 770, 812
SduI GDGCHC 2 cut(s) 21, 1086
SfaNI GCATC 3 cut(s) 528, 859, 1084
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 4 cut(s) 4, 610, 713, 772
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 5 cut(s) 163, 355, 398, 449, 1152
SsiI CCGC 4 cut(s) 587, 684, 966, 996
SspI AATATT 1 cut(s) 693
SspMI CTAG 1 cut(s) 953
StyD4I CCNGG 2 cut(s) 768, 810
StyI CCWWGG 1 cut(s) 973
TaaI ACNGT 3 cut(s) 441, 607, 1079
TaqI TCGA 4 cut(s) 273, 405, 561, 1157
TasI AATT 5 cut(s) 163, 355, 398, 449, 1152
TatI WGTACW 3 cut(s) 76, 552, 1062
TfiI GAWTC 5 cut(s) 112, 158, 323, 623, 980
Tru1I TTAA 5 cut(s) 462, 593, 600, 819, 846
Tru9I TTAA 5 cut(s) 462, 593, 600, 819, 846
TscAI CASTG 3 cut(s) 26, 1030, 1168
TseI GCWGC 3 cut(s) 723, 963, 1097
TspDTI ATGAA 2 cut(s) 171, 1016
TspGWI ACGGA 2 cut(s) 332, 408
TspRI CASTG 3 cut(s) 26, 1030, 1168
Van91I CCANNNNNTGG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 1082
VpaK11BI GGWCC 4 cut(s) 4, 610, 713, 772
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 2 cut(s) 163, 355
XceI RCATGY 1 cut(s) 1173
XspI CTAG 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.