RLG00000006752

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003753
Physical Location & Seq
Reverse (-)
10192754 .. 10195738
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006752

Sequence Viewer

Length: 1290 bp
ATGGACGGCGTGAGGCCGGAAGCAGGTGACCTGGACCAGAATGGTTGTCTAAAGCTTTCCAATGAGGTAGCTCATGACTTTAACAGACAGCTCAAGGAGACGGTGTTTAAACTAAGAACCCAACTTCCAGATGCACTTCTTACTTATGTCGATCTCTTTGCAGCAAAATATACACATATTAGTGAAGCACAAAAGTATGTCAGCTTGGCCTCAACTTCATGTAACTTCCCGGCCGTATTCAACTTCGGCGACTCAAACTCCGACACCGGTGGCTTGTCTGCAGTCTTCGGCCAGGCACGTTCACCTCACGGCGAGTCCTTCTTCCACGGCCCGGCTGGCCGGTACTGTGACGGCCGCCTCGTCATTGATTTTATAGCTAAAAGCCTTGAACTACCATATCTTAGCGCATATCTCGACTCGGTGGGAAGCAACTTCACTCACGGAGCCAACTTCGCGACGGCCGGATCGACCATCCGGCCTCAGAACACAACTATTCAACAGAGTGGGTTTAGTCCCATCTCCTTAAACGTTCAGTACAACGAGTTCTATGATTTTCTTCCTAGGTCCCAAGTTGCTCGGAATCGAGGTGGCGTTTTTGCACAACTAATGCCTAAAGCTGAGGATTTCTCTCGTGCCTTGTACACCTTCGATATTGGTCAGAACGACTTGACAGCCGGATTGTTCTTGAACATGTCCGTCGCCCAAGTTAAAGCATTTGTTCCTGATGTACTCGATAAGTTCAAAGACGTCGTCAAGTATGTACACAGTCAAGGAGGTAGATTTTTCTGGATACACAACACGGGACCGGTCGGTTGTCTACCCTATGTATTGGACCGCCTACCGGTCTTACCCTCGGAGGTGGACCGCCACGGGTGCGCCACACCTTTCAATGAACTTGCTCAGTTTTTCAACCGTGGATTAAAGCACGCTGTGGTCCAGTTAAGGAAAGAGCTACCTTCGGCTGCAATCACATATGTTGATGTTTACAAGGCCAAGTTCAACCTCATTAGCCAACCACAAAAGCATGGAGTTGAAGAACCGATAAGAGCATGTTGCGGCCATGGTGGGAAGTACAATTTCAATCCTCACCTTGGATGTGGAGGAAAGATCACGGTTCATGGCAAAGAAATATTAGTGGGAAAGTCATGTAAAGACCCTTCGGTTTGGGTAAATTGGGATGGGGTACACTACACTCAAGCAGCTAACAAAAAGATCTTCGATCAGATTGTAGATGGTTCATTTTCGAACCCTCCAGTTCCTTTGAAAATGGCTTGCCATAAGCACCACTAA

Protein Analysis

430

Amino Acids

47.37

Weight (kDa)

8.24

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 79 - 409 6.9e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
AatII GACGTC 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 14
AccI GTMKAC 1 cut(s) 817
AccII CGCG 1 cut(s) 455
AciI CCGC 4 cut(s) 355, 835, 865, 1056
AclI AACGTT 1 cut(s) 528
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 6 cut(s) 231, 289, 337, 352, 459, 1057
AcyI GRCGYC 1 cut(s) 747
AdeI CACNNNGTG 1 cut(s) 931
AfaI GTAC 7 cut(s) 344, 536, 641, 729, 762, 1073, 1185
AfiI CCNNNNNNNGG 4 cut(s) 23, 331, 841, 1091
AflIII ACRYGT 1 cut(s) 690
AgeI ACCGGT 3 cut(s) 266, 805, 841
AjnI CCWGG 2 cut(s) 30, 291
AloI GAACNNNNNNTCC 2 cut(s) 109, 141
AluBI AGCT 8 cut(s) 55, 71, 91, 204, 377, 617, 952, 1202
AluI AGCT 8 cut(s) 55, 71, 91, 204, 377, 617, 952, 1202
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 472
ApeKI GCWGC 3 cut(s) 161, 962, 1199
ArsI GACNNNNNNTTYG 2 cut(s) 735, 767
AsiGI ACCGGT 3 cut(s) 266, 805, 841
AspA2I CCTAGG 1 cut(s) 560
AspLEI GCGC 2 cut(s) 407, 878
AspS9I GGNCC 7 cut(s) 34, 329, 564, 803, 832, 862, 934
AsuC2I CCSGG 2 cut(s) 230, 332
AsuHPI GGTGA 3 cut(s) 38, 294, 1079
AsuII TTCGAA 1 cut(s) 1244
AvaII GGWCC 6 cut(s) 34, 564, 803, 832, 862, 934
AvrII CCTAGG 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 1141, 1173
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 277
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 3 cut(s) 173, 949, 1211
BccI CCATC 4 cut(s) 479, 524, 1172, 1226
BceAI ACGGC 6 cut(s) 22, 218, 325, 343, 367, 474
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 2 cut(s) 32, 293
BciVI GTATCC 1 cut(s) 783
BcnI CCSGG 2 cut(s) 230, 332
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 783
BglI GCCNNNNNGGC 1 cut(s) 336
BglII AGATCT 1 cut(s) 1212
BisI GCNGC 5 cut(s) 162, 355, 963, 1057, 1200
BlnI CCTAGG 1 cut(s) 560
BlsI GCNGC 5 cut(s) 163, 356, 964, 1058, 1201
Bme1390I CCNGG 4 cut(s) 32, 230, 293, 332
Bme18I GGWCC 6 cut(s) 34, 564, 803, 832, 862, 934
BmgT120I GGNCC 7 cut(s) 34, 329, 564, 803, 832, 862, 934
BmiI GGNNCC 3 cut(s) 445, 566, 804
BmrFI CCNGG 4 cut(s) 32, 230, 293, 332
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 277
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 1 cut(s) 1236
Bpu10I CCTNAGC 1 cut(s) 618
Bpu14I TTCGAA 1 cut(s) 1244
BpuEI CTTGAG 2 cut(s) 77, 1179
BpuMI CCSGG 2 cut(s) 230, 332
BsaHI GRCGYC 1 cut(s) 747
BsaJI CCNNGG 7 cut(s) 325, 560, 852, 868, 913, 1060, 1090
BsaWI WCCGGW 3 cut(s) 266, 805, 841
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 242, 272
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 331, 841, 1091
Bse118I RCCGGY 4 cut(s) 266, 339, 805, 841
Bse1I ACTGG 2 cut(s) 937, 1253
BseBI CCWGG 2 cut(s) 32, 293
BseDI CCNNGG 7 cut(s) 325, 560, 852, 868, 913, 1060, 1090
BseGI GGATG 3 cut(s) 471, 1100, 1183
BseLI CCNNNNNNNGG 4 cut(s) 23, 331, 841, 1091
BseMII CTCAG 3 cut(s) 494, 609, 914
BseNI ACTGG 2 cut(s) 937, 1253
BseX3I CGGCCG 3 cut(s) 231, 352, 459
BseXI GCAGC 3 cut(s) 173, 949, 1211
Bsh1236I CGCG 1 cut(s) 455
Bsh1285I CGRYCG 4 cut(s) 234, 355, 462, 810
BshTI ACCGGT 3 cut(s) 266, 805, 841
BsiEI CGRYCG 4 cut(s) 234, 355, 462, 810
BslFI GGGAC 3 cut(s) 498, 550, 816
BslI CCNNNNNNNGG 4 cut(s) 23, 331, 841, 1091
BsmAI GTCTC 1 cut(s) 92
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 498, 550, 816
Bsp119I TTCGAA 1 cut(s) 1244
Bsp1407I TGTACA 2 cut(s) 639, 760
Bsp143I GATC 5 cut(s) 151, 464, 1107, 1212, 1219
Bsp19I CCATGG 1 cut(s) 1060
Bsp68I TCGCGA 1 cut(s) 455
BspACI CCGC 4 cut(s) 355, 835, 865, 1056
BspCNI CTCAG 3 cut(s) 493, 610, 913
BspFNI CGCG 1 cut(s) 455
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 3 cut(s) 445, 566, 804
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 472
BspT104I TTCGAA 1 cut(s) 1244
BsrFI RCCGGY 4 cut(s) 266, 339, 805, 841
BsrGI TGTACA 2 cut(s) 639, 760
BsrI ACTGG 2 cut(s) 937, 1253
BssAI RCCGGY 4 cut(s) 266, 339, 805, 841
BssECI CCNNGG 7 cut(s) 325, 560, 852, 868, 913, 1060, 1090
BssMI GATC 5 cut(s) 151, 464, 1107, 1212, 1219
BssNI GRCGYC 1 cut(s) 747
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 3 cut(s) 560, 1060, 1090
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 32, 293
Bst4CI ACNGT 5 cut(s) 103, 347, 767, 914, 1114
BstACI GRCGYC 1 cut(s) 747
BstAUI TGTACA 2 cut(s) 639, 760
BstBI TTCGAA 1 cut(s) 1244
BstC8I GCNNGC 3 cut(s) 337, 927, 1273
BstDEI CTNAG 5 cut(s) 113, 401, 480, 618, 900
BstDSI CCRYGG 4 cut(s) 325, 868, 913, 1060
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 3 cut(s) 471, 1100, 1183
BstFNI CGCG 1 cut(s) 455
BstHHI GCGC 2 cut(s) 407, 878
BstKTI GATC 5 cut(s) 154, 467, 1110, 1215, 1222
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 151, 464, 1107, 1212, 1219
BstMCI CGRYCG 4 cut(s) 234, 355, 462, 810
BstMWI GCNNNNNNNGC 3 cut(s) 336, 452, 873
BstNI CCWGG 2 cut(s) 32, 293
BstNSI RCATGY 2 cut(s) 694, 1053
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 4 cut(s) 30, 228, 291, 330
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 3 cut(s) 173, 949, 1211
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 1212
BstYI RGATCY 1 cut(s) 1212
BstZI CGGCCG 3 cut(s) 231, 352, 459
BsuI GTATCC 1 cut(s) 783
BtgI CCRYGG 4 cut(s) 325, 868, 913, 1060
BtsCI GGATG 3 cut(s) 471, 1100, 1183
BtuMI TCGCGA 1 cut(s) 455
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 3 cut(s) 337, 927, 1273
CciI TCATGA 1 cut(s) 73
CfoI GCGC 2 cut(s) 407, 878
Cfr10I RCCGGY 4 cut(s) 266, 339, 805, 841
Cfr13I GGNCC 7 cut(s) 34, 329, 564, 803, 832, 862, 934
Csp6I GTAC 7 cut(s) 343, 535, 640, 728, 761, 1072, 1184
CspAI ACCGGT 3 cut(s) 266, 805, 841
CviAII CATG 8 cut(s) 74, 219, 691, 1025, 1050, 1061, 1118, 1146
CviQI GTAC 7 cut(s) 343, 535, 640, 728, 761, 1072, 1184
DdeI CTNAG 5 cut(s) 113, 401, 480, 618, 900
DpnI GATC 5 cut(s) 153, 466, 1109, 1214, 1221
DpnII GATC 5 cut(s) 151, 464, 1107, 1212, 1219
DraI TTTAAA 1 cut(s) 109
DraIII CACNNNGTG 1 cut(s) 931
EaeI YGGCCR 6 cut(s) 231, 289, 337, 352, 459, 1057
EagI CGGCCG 3 cut(s) 231, 352, 459
EclXI CGGCCG 3 cut(s) 231, 352, 459
Eco130I CCWWGG 3 cut(s) 560, 1060, 1090
Eco47I GGWCC 6 cut(s) 34, 564, 803, 832, 862, 934
Eco52I CGGCCG 3 cut(s) 231, 352, 459
Eco91I GGTNACC 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 564
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 30, 291
EcoT14I CCWWGG 3 cut(s) 560, 1060, 1090
ErhI CCWWGG 3 cut(s) 560, 1060, 1090
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 8 cut(s) 77, 222, 694, 1028, 1053, 1064, 1121, 1149
FaqI GGGAC 3 cut(s) 498, 550, 816
FatI CATG 8 cut(s) 73, 218, 690, 1024, 1049, 1060, 1117, 1145
FauNDI CATATG 1 cut(s) 973
FblI GTMKAC 1 cut(s) 817
Fnu4HI GCNGC 5 cut(s) 162, 355, 963, 1057, 1200
FokI GGATG 3 cut(s) 458, 1107, 1190
Fsp4HI GCNGC 5 cut(s) 162, 355, 963, 1057, 1200
FspBI CTAG 1 cut(s) 561
GlaI GCGC 2 cut(s) 406, 877
GluI GCNGC 5 cut(s) 162, 355, 963, 1057, 1200
GsuI CTGGAG 1 cut(s) 1236
HhaI GCGC 2 cut(s) 407, 878
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 8 cut(s) 77, 222, 694, 1028, 1053, 1064, 1121, 1149
Hin6I GCGC 2 cut(s) 405, 876
HinP1I GCGC 2 cut(s) 405, 876
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 4 cut(s) 251, 314, 416, 580
HphI GGTGA 3 cut(s) 38, 294, 1079
Hpy166II GTNNAC 7 cut(s) 302, 642, 763, 818, 862, 985, 1186
Hpy188I TCNGA 6 cut(s) 262, 483, 579, 660, 856, 1224
Hpy188III TCNNGA 7 cut(s) 74, 128, 413, 454, 685, 722, 787
Hpy8I GTNNAC 7 cut(s) 302, 642, 763, 818, 862, 985, 1186
Hpy99I CGWCG 3 cut(s) 460, 701, 752
HpyAV CCTTC 4 cut(s) 328, 655, 966, 1167
HpyCH4III ACNGT 5 cut(s) 103, 347, 767, 914, 1114
HpyCH4IV ACGT 3 cut(s) 298, 528, 747
HpyCH4V TGCA 5 cut(s) 134, 161, 281, 599, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 452, 873
HpyF3I CTNAG 5 cut(s) 113, 401, 480, 618, 900
HpySE526I ACGT 3 cut(s) 298, 528, 747
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 8 cut(s) 77, 222, 694, 1028, 1053, 1064, 1121, 1149
HspAI GCGC 2 cut(s) 405, 876
Kzo9I GATC 5 cut(s) 151, 464, 1107, 1212, 1219
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 3 cut(s) 173, 949, 1211
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 561
MaeII ACGT 3 cut(s) 298, 528, 747
MaeIII GTNAC 3 cut(s) 26, 221, 347
MalI GATC 5 cut(s) 153, 466, 1109, 1214, 1221
MboI GATC 5 cut(s) 151, 464, 1107, 1212, 1219
MboII GAAGA 5 cut(s) 277, 313, 548, 1046, 1207
MflI RGATCY 1 cut(s) 1212
MluCI AATT 2 cut(s) 1075, 1171
MlyI GAGTC 3 cut(s) 245, 323, 410
MmeI TCCRAC 1 cut(s) 285
MseI TTAA 6 cut(s) 81, 108, 524, 708, 920, 941
MslI CAYNNNNRTG 1 cut(s) 180
MspR9I CCNGG 4 cut(s) 32, 230, 293, 332
MssI GTTTAAAC 1 cut(s) 109
MvaI CCWGG 2 cut(s) 32, 293
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 336, 452, 873
NciI CCSGG 2 cut(s) 230, 332
NcoI CCATGG 1 cut(s) 1060
NdeI CATATG 1 cut(s) 973
NdeII GATC 5 cut(s) 151, 464, 1107, 1212, 1219
NlaIII CATG 8 cut(s) 77, 222, 694, 1028, 1053, 1064, 1121, 1149
NlaIV GGNNCC 3 cut(s) 445, 566, 804
NmuCI GTSAC 2 cut(s) 26, 347
NruI TCGCGA 1 cut(s) 455
NspI RCATGY 2 cut(s) 694, 1053
NspV TTCGAA 1 cut(s) 1244
PagI TCATGA 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 14
PciI ACATGT 1 cut(s) 690
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 464
PfeI GAWTC 1 cut(s) 580
PflFI GACNNNGTC 1 cut(s) 749
PinAI ACCGGT 3 cut(s) 266, 805, 841
PkrI GCNGC 5 cut(s) 163, 356, 964, 1058, 1201
PleI GAGTC 3 cut(s) 245, 322, 410
PmeI GTTTAAAC 1 cut(s) 109
PpsI GAGTC 3 cut(s) 245, 322, 410
PpuMI RGGWCCY 1 cut(s) 564
PscI ACATGT 1 cut(s) 690
Psp1406I AACGTT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 30, 291
PspEI GGTNACC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 30, 291
PspN4I GGNNCC 3 cut(s) 445, 566, 804
PspPI GGNCC 7 cut(s) 34, 329, 564, 803, 832, 862, 934
PspPPI RGGWCCY 1 cut(s) 564
PsrI GAACNNNNNNTAC 2 cut(s) 527, 559
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 1212
PsyI GACNNNGTC 1 cut(s) 749
RruI TCGCGA 1 cut(s) 455
RsaI GTAC 7 cut(s) 344, 536, 641, 729, 762, 1073, 1185
RsaNI GTAC 7 cut(s) 343, 535, 640, 728, 761, 1072, 1184
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 6 cut(s) 81, 108, 524, 708, 920, 941
SatI GCNGC 5 cut(s) 162, 355, 963, 1057, 1200
Sau3AI GATC 5 cut(s) 151, 464, 1107, 1212, 1219
Sau96I GGNCC 7 cut(s) 34, 329, 564, 803, 832, 862, 934
SchI GAGTC 3 cut(s) 245, 323, 410
ScrFI CCNGG 4 cut(s) 32, 230, 293, 332
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 279
SfiI GGCCNNNNNGGCC 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 1244
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 6 cut(s) 34, 564, 803, 832, 862, 934
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 2 cut(s) 92, 1194
SmoI CTYRAG 2 cut(s) 92, 1194
Sse9I AATT 2 cut(s) 1075, 1171
SsiI CCGC 4 cut(s) 355, 835, 865, 1056
SspI AATATT 1 cut(s) 1131
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 4 cut(s) 30, 228, 291, 330
StyI CCWWGG 3 cut(s) 560, 1060, 1090
TaaI ACNGT 5 cut(s) 103, 347, 767, 914, 1114
TaiI ACGT 3 cut(s) 301, 531, 750
TaqI TCGA 8 cut(s) 150, 414, 467, 583, 648, 732, 1218, 1244
TasI AATT 2 cut(s) 1075, 1171
TatI WGTACW 5 cut(s) 534, 639, 727, 760, 1071
TauI GCSGC 2 cut(s) 357, 1059
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 6 cut(s) 81, 108, 524, 708, 920, 941
Tru9I TTAA 6 cut(s) 81, 108, 524, 708, 920, 941
TseFI GTSAC 2 cut(s) 26, 347
TseI GCWGC 3 cut(s) 161, 962, 1199
Tsp45I GTSAC 2 cut(s) 26, 347
TspDTI ATGAA 4 cut(s) 207, 906, 1106, 1227
TspGWI ACGGA 2 cut(s) 456, 685
Tth111I GACNNNGTC 1 cut(s) 749
VpaK11BI GGWCC 6 cut(s) 34, 564, 803, 832, 862, 934
XceI RCATGY 2 cut(s) 694, 1053
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XmaJI CCTAGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 817
XspI CTAG 1 cut(s) 561
ZraI GACGTC 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.