Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003753

Physical Location & Seq

Genomic Coordinates Forward (+)

54729784 .. 54735134

Gene Structure 5351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G028030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGGACCCTGTGAGAGGGCACTGGGGACTGAAGAGGAGTGTGGAAGCAGTAGCAGTTGGAGTTGTTTTCGTTGGTGTACTTTCAGTTTTTCTGGGCCTCAGACCCCAAAAGATTCAATCAAGTCGTTGTCATTTTCCGGCAATCTATAACTTTGGCGATTCAAATTCCGACACAGGTGCTTCATCAGCTGTGTTTTATCGGCTTCCTTCTCCCTATGGCAATACATTCTTTGGCAAACCTTCGGGGAGGTTATCCGATGGGCGTCTCATGATCGATTTTATAGCTCAGAAGTTGGGTTTGCCATTTTTGAGTGCTTATCTTGATTCTGTTGGAGCAAACTTCCGTCATGGAGCAAATTTTGCAACGGGAGGATCTACAATCTTCCCAATAGACGGAAAATTGTTCGAAGGAAGATTTAGCCCCATCTCTCTTGACAAACAGTTTTCGCAATTTGCCCAGCTTAAAGCTCGTGTAAATGAGGTTCTAAGTCAAGATAAAAGGTCATATGTCAGCGCCAGCATCCCCAGACCAGAGGACTTCTCAAAGGCATTGTACACCTTCGACATGGGACAGAACGATATTCATGCGGGATTAAGGTTTAAAACGGTGGACCAAGTGCTGGAATCCATTTCCGACATGACAGCTCAGTTAGCTTTGACAATAGAGCAACTATACCAGCAAGGCGGAAGAATATTTTGGATCCATAACACGGGTCCACTCGGCTGCTTGCCTATGTGTCTTGCACATAATCAGCCAGAACCCGATGACCTGGACCAGAATGGCTGTTTGAAGTCTTACAATGAGGTGGCCCAGGAGTTTAACAGACAGCTCAAGGAAAGAGTGCTTAATCTAAGAGCCAAGCTCTCGGATGCAGTTCTTACATATGTTGATCTCTATAGAGCAAAATACACGCTTATTAGTGAAACAGAGAAGCATGGTTTTAGTGATCCTCTAGCTCAATGCTGCGGACGCCAAGGTGATTCTCCTTTCCATTGCGGGATAAATACTACTGCTGTCAATGGCACTGAAGTATTTGGTGGCTCTTGTAGTAATCCTGCAAAGTACATTAGCTGGGACGGTGTGCACTATTCGGATGCTGCAAACAAATGGGTAGCCAAACGAGTTATAGACGGCTCATTCTCAGACCCTCCAATTTCGATCACTGAAGCATACCCCTCTCTCATTATGGAATCACATTCCTCAACAATACTAGCAGTTCTCACTCTTCTGGTGTCACTGTCTGCAGTCAGCTTGGCCTCAACTAATTCATGTAACTTCCCAGCCGTATTCAACTTCGGCGACTCAAACTCCGACACCGGTGGCTTGTCTGCAGTCTTCGGCCAGGCACGTTCACCTCACGGCGAGTCCTTCTTCCACGGCCCGGCTGGCCGGTACTGTGACGGCCGCCTCCTCATTGATTTTGTAGCTAAAAGCCTTGGACTACCATATCTTAGCGCATATCTCGACTCGGTGGGAAGCAACTTCACTCACGGAGCCAACTTCGCGACGGCGGGATCGACCATCCGGCCTCAGAACACAACTATTCAACAGAGTGGGTTTAGTCCCATCTCCTTAAACGTTCAGTACAACGAGTTCTATGATTTTCTTCCTAGGTCCCAAGTTGCTCGGAATCGAGGTGGAGTTTTTGCACAACTAATGCCCAAAGCTGAGGATTTCTCTCGTGCCTTGTACACCTTCGATATTGGTCAGAACGACTTGACAGCCGGTTTGTTCTTGAACATGTCCGTCGCCCAAGTTAAAGCATTTGTTCCTGATGTACTCGATAAGTTCAAAGACGTCGTCAAGTATGTACACAGTCAAGGAGGTAGATTTTTCTGGATACACAACACGGGACCGGTCGGTTGTCTACCCTATGTATTGGACCGCCTACCGGTCTTACCCTCGGAGGTGGACCGCCACGGGTGCGCCACACCTTTCAATGAACTTGCTCAGTTTTTCAACCGTGGATTAAAGCACGCTGTGGTCCAGTTAAGGAAAGAGCTACCTTCGGCTGCAATCACATATGTTGATGTTTACAAGGCCAAGTTCAACCTCATTAGCCAACCACAAAAGCATGGAGTTAAAGAACCGATAAGAGCATGTTGCGGCCATGGTGGGAAGTACAATTTCAATCCGCACCTTGGATGTGGAGGAAAGATCAAGGTTCATGGCAAAGAAATATTAGTGGGAAAGTCATGTAAAGACCCTTCGGTTTGGGTAAATTGGGATGGGGTACATTACACTCAAGCAGCTAACAAAAAGATCTTCGATCAGATTGTAGATGGTTCATTTTCGAACCCTCCAGTTCCTTTGAAAATGGCTTGCCATAAGCACCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0015928 GO:0016298 GO:0016787 GO:0016788 GO:0016798 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Lipase_GDSL

Protein Analysis

779

Amino Acids

85.48

Weight (kDa)

8.5

Isoelectric Point (pI)

37.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_GDSL	PF00657	48 - 375	7.9e-45	GDSL-like Lipase/Acylhydrolase
Lipase_GDSL	PF00657	429 - 759	7.4e-45	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67830
fragaria_vesca	FvH4_4g26010 FvH4_4g26011 FvH4_4g26011 FvH4_4g26011 FvH4_4g26011 FvH4_4g26011
malus_domestica	MD11G1248900.v1.1 MD13G1098700.v1.1 MD13G1098900.v1.1 MD16G1099800.v1.1 MD16G1100200.v1.1
prunus_persica	Prupe.1G246700_v2.0.a1
pyrus_communis	pycom13g08640 pycom16g08480 pycom16g08490
rosa_chinensis	RchiOBHm_Chr4g0433431 RchiOBHm_Chr4g0433451
rosa_laevigata	RLG00000006752 RLG00000006753
rosa_multiflora	Rmu_co8258823.1_g000001 Rmu_co8425109.1_g000001 Rmu_sc0000759.1_g000003
rosa_roxburghii	Rroxscaffold_5G00374980 Rroxscaffold_5G00374990
rosa_rugosa	Rorug04G0268800 Rorug04G0268900
rosa_samantha	Rh4BG331400 Rh4BG331500 Rh4CG346700 Rh4CG346800 Rh4DG327700 Rh4DG327800
rosa_wichuraiana	Rw4G028030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1800
AccB7I	CCANNNNNTGG	1 cut(s)	619
AccI	GTMKAC	1 cut(s)	1867
AccII	CGCG	1 cut(s)	1505
AclI	AACGTT	1 cut(s)	1578
AclWI	GGATC	5 cut(s)	379, 694, 707, 941, 1522
AcoI	YGGCCR	4 cut(s)	1339, 1387, 1402, 2107
AcsI	RAATTY	2 cut(s)	163, 355
AcuI	CTGAAG	3 cut(s)	50, 1047, 1185
AcyI	GRCGYC	3 cut(s)	262, 970, 1797
AdeI	CACNNNGTG	1 cut(s)	1981
AfiI	CCNNNNNNNGG	7 cut(s)	14, 392, 619, 709, 1381, 1891, 2141
AflIII	ACRYGT	1 cut(s)	1740
AgeI	ACCGGT	3 cut(s)	1316, 1855, 1891
AjnI	CCWGG	3 cut(s)	768, 810, 1341
AjuI	GAANNNNNNNTTGG	2 cut(s)	679, 711
Alw21I	GWGCWC	1 cut(s)	1086
Alw26I	GTCTC	1 cut(s)	269
Alw44I	GTGCAC	1 cut(s)	1082
AlwI	GGATC	5 cut(s)	379, 694, 707, 941, 1522
ApaLI	GTGCAC	1 cut(s)	1082
ApeKI	GCWGC	5 cut(s)	723, 963, 1097, 2012, 2249
ApoI	RAATTY	2 cut(s)	163, 355
ArsI	GACNNNNNNTTYG	4 cut(s)	1711, 1743, 1785, 1817
AsiGI	ACCGGT	3 cut(s)	1316, 1855, 1891
AspA2I	CCTAGG	1 cut(s)	1610
AspLEI	GCGC	3 cut(s)	515, 1457, 1928
AsuC2I	CCSGG	1 cut(s)	1382
AsuHPI	GGTGA	2 cut(s)	989, 1344
AsuII	TTCGAA	2 cut(s)	405, 2294
AvaII	GGWCC	9 cut(s)	4, 610, 713, 772, 1614, 1853, 1882, 1912, 1984
AvrII	CCTAGG	1 cut(s)	1610
BaeGI	GKGCMC	2 cut(s)	21, 1086
BamHI	GGATCC	1 cut(s)	699
BarI	GAAGNNNNNNTAC	2 cut(s)	2191, 2223
BauI	CACGAG	2 cut(s)	468, 1680
BbsI	GAAGAC	1 cut(s)	1327
Bbv12I	GWGCWC	1 cut(s)	1086
BbvCI	CCTCAGC	1 cut(s)	1668
BbvI	GCAGC	5 cut(s)	710, 950, 1084, 1999, 2261
BccI	CCATC	6 cut(s)	251, 431, 1529, 1574, 2222, 2276
BceAI	ACGGC	6 cut(s)	1147, 1268, 1375, 1393, 1417, 1524
BcgI	CGANNNNNNTGC	4 cut(s)	158, 192, 566, 600
BciT130I	CCWGG	3 cut(s)	770, 812, 1343
BciVI	GTATCC	1 cut(s)	1833
BcnI	CCSGG	1 cut(s)	1382
BcoDI	GTCTC	1 cut(s)	269
BfaI	CTAG	3 cut(s)	953, 1211, 1611
BfmI	CTRYAG	3 cut(s)	895, 1242, 1329
BfoI	RGCGCY	1 cut(s)	516
BfuI	GTATCC	1 cut(s)	1833
BglI	GCCNNNNNGGC	1 cut(s)	1386
BglII	AGATCT	1 cut(s)	2262
BisI	GCNGC	7 cut(s)	724, 964, 1098, 1405, 2013, 2107, 2250
BlnI	CCTAGG	1 cut(s)	1610
BlsI	GCNGC	7 cut(s)	725, 965, 1099, 1406, 2014, 2108, 2251
Bme1390I	CCNGG	4 cut(s)	770, 812, 1343, 1382
Bme18I	GGWCC	9 cut(s)	4, 610, 713, 772, 1614, 1853, 1882, 1912, 1984
BmiI	GGNNCC	6 cut(s)	6, 701, 714, 1495, 1616, 1854
BmrFI	CCNGG	4 cut(s)	770, 812, 1343, 1382
BmrI	ACTGGG	1 cut(s)	31
BmsI	GCATC	3 cut(s)	528, 859, 1084
BmuI	ACTGGG	1 cut(s)	31
BpiI	GAAGAC	1 cut(s)	1327
BplI	GAGNNNNNCTC	6 cut(s)	524, 556, 846, 878, 1661, 1693
BpmI	CTGGAG	1 cut(s)	2286
Bpu10I	CCTNAGC	1 cut(s)	1668
Bpu14I	TTCGAA	2 cut(s)	405, 2294
BpuEI	CTTGAG	2 cut(s)	815, 2229
BpuMI	CCSGG	1 cut(s)	1382
Bsa29I	ATCGAT	1 cut(s)	273
BsaHI	GRCGYC	3 cut(s)	262, 970, 1797
BsaWI	WCCGGW	3 cut(s)	1316, 1855, 1891
BsaXI	ACNNNNNCTCC	2 cut(s)	1292, 1322
Bsc4I	CCNNNNNNNGG	7 cut(s)	14, 392, 619, 709, 1381, 1891, 2141
Bse118I	RCCGGY	5 cut(s)	1316, 1389, 1724, 1855, 1891
Bse1I	ACTGG	3 cut(s)	26, 1987, 2303
Bse3DI	GCAATG	1 cut(s)	991
BseBI	CCWGG	3 cut(s)	770, 812, 1343
BseCI	ATCGAT	1 cut(s)	273
BseGI	GGATG	6 cut(s)	519, 874, 1099, 1521, 2150, 2233
BseLI	CCNNNNNNNGG	7 cut(s)	14, 392, 619, 709, 1381, 1891, 2141
BseMI	GCAATG	1 cut(s)	991
BseMII	CTCAG	7 cut(s)	112, 299, 659, 1155, 1544, 1659, 1964
BseNI	ACTGG	3 cut(s)	26, 1987, 2303
BseRI	GAGGAG	2 cut(s)	49, 1400
BseSI	GKGCMC	2 cut(s)	21, 1086
BseX3I	CGGCCG	1 cut(s)	1402
BseXI	GCAGC	5 cut(s)	710, 950, 1084, 1999, 2261
BseYI	CCCAGC	3 cut(s)	456, 1071, 1279
Bsh1236I	CGCG	1 cut(s)	1505
Bsh1285I	CGRYCG	2 cut(s)	1405, 1860
BshTI	ACCGGT	3 cut(s)	1316, 1855, 1891
BshVI	ATCGAT	1 cut(s)	273
BsiEI	CGRYCG	2 cut(s)	1405, 1860
BsiHKAI	GWGCWC	1 cut(s)	1086
BsiSI	CCGG	8 cut(s)	137, 1317, 1382, 1390, 1525, 1725, 1856, 1892
BslFI	GGGAC	6 cut(s)	39, 582, 1088, 1548, 1600, 1866
BslI	CCNNNNNNNGG	7 cut(s)	14, 392, 619, 709, 1381, 1891, 2141
BsmAI	GTCTC	1 cut(s)	269
BsmBI	CGTCTC	1 cut(s)	269
BsmFI	GGGAC	6 cut(s)	39, 582, 1088, 1548, 1600, 1866
Bsp119I	TTCGAA	2 cut(s)	405, 2294
Bsp1286I	GDGCHC	2 cut(s)	21, 1086
Bsp1407I	TGTACA	3 cut(s)	552, 1689, 1810
Bsp19I	CCATGG	1 cut(s)	2110
Bsp68I	TCGCGA	1 cut(s)	1505
BspCNI	CTCAG	7 cut(s)	111, 298, 658, 1154, 1543, 1660, 1963
BspDI	ATCGAT	1 cut(s)	273
BspFNI	CGCG	1 cut(s)	1505
BspHI	TCATGA	1 cut(s)	267
BspLI	GGNNCC	6 cut(s)	6, 701, 714, 1495, 1616, 1854
BspMAI	CTGCAG	2 cut(s)	1246, 1333
BspPI	GGATC	5 cut(s)	379, 694, 707, 941, 1522
BspT104I	TTCGAA	2 cut(s)	405, 2294
BsrDI	GCAATG	1 cut(s)	991
BsrFI	RCCGGY	5 cut(s)	1316, 1389, 1724, 1855, 1891
BsrGI	TGTACA	3 cut(s)	552, 1689, 1810
BsrI	ACTGG	3 cut(s)	26, 1987, 2303
BssAI	RCCGGY	5 cut(s)	1316, 1389, 1724, 1855, 1891
BssNI	GRCGYC	3 cut(s)	262, 970, 1797
BssSI	CACGAG	2 cut(s)	468, 1680
BssT1I	CCWWGG	5 cut(s)	973, 1435, 1610, 2110, 2140
Bst2BI	CACGAG	2 cut(s)	468, 1680
Bst2UI	CCWGG	3 cut(s)	770, 812, 1343
Bst4CI	ACNGT	7 cut(s)	441, 607, 1079, 1239, 1397, 1817, 1964
Bst6I	CTCTTC	2 cut(s)	26, 1230
BstACI	GRCGYC	3 cut(s)	262, 970, 1797
BstAPI	GCANNNNNTGC	1 cut(s)	359
BstAUI	TGTACA	3 cut(s)	552, 1689, 1810
BstBI	TTCGAA	2 cut(s)	405, 2294
BstC8I	GCNNGC	5 cut(s)	517, 728, 1387, 1977, 2323
BstDSI	CCRYGG	4 cut(s)	1375, 1918, 1963, 2110
BstENI	CCTNNNNNAGG	1 cut(s)	12
BstF5I	GGATG	6 cut(s)	519, 874, 1099, 1521, 2150, 2233
BstFNI	CGCG	1 cut(s)	1505
BstH2I	RGCGCY	1 cut(s)	516
BstHHI	GCGC	3 cut(s)	515, 1457, 1928
BstMAI	GTCTC	1 cut(s)	269
BstMCI	CGRYCG	2 cut(s)	1405, 1860
BstMWI	GCNNNNNNNGC	7 cut(s)	185, 359, 650, 969, 1386, 1502, 1923
BstNI	CCWGG	3 cut(s)	770, 812, 1343
BstNSI	RCATGY	2 cut(s)	1744, 2103
BstSCI	CCNGG	4 cut(s)	768, 810, 1341, 1380
BstSFI	CTRYAG	3 cut(s)	895, 1242, 1329
BstSLI	GKGCMC	2 cut(s)	21, 1086
BstUI	CGCG	1 cut(s)	1505
BstV1I	GCAGC	5 cut(s)	710, 950, 1084, 1999, 2261
BstV2I	GAAGAC	1 cut(s)	1327
BstX2I	RGATCY	3 cut(s)	371, 699, 2262
BstYI	RGATCY	3 cut(s)	371, 699, 2262
BstZI	CGGCCG	1 cut(s)	1402
Bsu15I	ATCGAT	1 cut(s)	273
BsuI	GTATCC	1 cut(s)	1833
BsuTUI	ATCGAT	1 cut(s)	273
BtgI	CCRYGG	4 cut(s)	1375, 1918, 1963, 2110
BtsCI	GGATG	6 cut(s)	519, 874, 1099, 1521, 2150, 2233
BtsIMutI	CAGTG	4 cut(s)	19, 1023, 1161, 1235
BtuMI	TCGCGA	1 cut(s)	1505
Cac8I	GCNNGC	5 cut(s)	517, 728, 1387, 1977, 2323
CciI	TCATGA	1 cut(s)	267
CfoI	GCGC	3 cut(s)	515, 1457, 1928
Cfr10I	RCCGGY	5 cut(s)	1316, 1389, 1724, 1855, 1891
ClaI	ATCGAT	1 cut(s)	273
CseI	GACGC	2 cut(s)	251, 978
CspAI	ACCGGT	3 cut(s)	1316, 1855, 1891
DraI	TTTAAA	1 cut(s)	601
DraIII	CACNNNGTG	1 cut(s)	1981
EaeI	YGGCCR	4 cut(s)	1339, 1387, 1402, 2107
EagI	CGGCCG	1 cut(s)	1402
Eam1104I	CTCTTC	2 cut(s)	26, 1230
EarI	CTCTTC	2 cut(s)	26, 1230
EciI	GGCGGA	1 cut(s)	699
EclXI	CGGCCG	1 cut(s)	1402
Eco130I	CCWWGG	5 cut(s)	973, 1435, 1610, 2110, 2140
Eco47I	GGWCC	9 cut(s)	4, 610, 713, 772, 1614, 1853, 1882, 1912, 1984
Eco52I	CGGCCG	1 cut(s)	1402
Eco57I	CTGAAG	3 cut(s)	50, 1047, 1185
EcoNI	CCTNNNNNAGG	1 cut(s)	12
EcoO109I	RGGNCCY	1 cut(s)	1614
EcoRII	CCWGG	3 cut(s)	768, 810, 1341
EcoT14I	CCWWGG	5 cut(s)	973, 1435, 1610, 2110, 2140
ErhI	CCWWGG	5 cut(s)	973, 1435, 1610, 2110, 2140
Esp3I	CGTCTC	1 cut(s)	269
FaqI	GGGAC	6 cut(s)	39, 582, 1088, 1548, 1600, 1866
FauI	CCCGC	3 cut(s)	580, 989, 1504
FauNDI	CATATG	3 cut(s)	505, 883, 2023
FblI	GTMKAC	1 cut(s)	1867
Fnu4HI	GCNGC	7 cut(s)	724, 964, 1098, 1405, 2013, 2107, 2250
FokI	GGATG	6 cut(s)	506, 881, 1106, 1508, 2157, 2240
Fsp4HI	GCNGC	7 cut(s)	724, 964, 1098, 1405, 2013, 2107, 2250
FspBI	CTAG	3 cut(s)	953, 1211, 1611
GlaI	GCGC	3 cut(s)	514, 1456, 1927
GluI	GCNGC	7 cut(s)	724, 964, 1098, 1405, 2013, 2107, 2250
GsaI	CCCAGC	3 cut(s)	460, 1075, 1283
GsuI	CTGGAG	1 cut(s)	2286
HaeII	RGCGCY	1 cut(s)	516
HapII	CCGG	8 cut(s)	137, 1317, 1382, 1390, 1525, 1725, 1856, 1892
HgaI	GACGC	2 cut(s)	251, 978
HhaI	GCGC	3 cut(s)	515, 1457, 1928
Hin1I	GRCGYC	3 cut(s)	262, 970, 1797
Hin6I	GCGC	3 cut(s)	513, 1455, 1926
HinP1I	GCGC	3 cut(s)	513, 1455, 1926
HpaII	CCGG	8 cut(s)	137, 1317, 1382, 1390, 1525, 1725, 1856, 1892
HphI	GGTGA	2 cut(s)	989, 1344
Hpy188III	TCNNGA	9 cut(s)	268, 320, 431, 491, 1463, 1504, 1735, 1772, 1837
Hpy99I	CGWCG	3 cut(s)	1510, 1751, 1802
HpyAV	CCTTC	8 cut(s)	216, 249, 401, 568, 1378, 1705, 2016, 2217
HpyCH4III	ACNGT	7 cut(s)	441, 607, 1079, 1239, 1397, 1817, 1964
HpyCH4IV	ACGT	3 cut(s)	1348, 1578, 1797
HpyF10VI	GCNNNNNNNGC	7 cut(s)	185, 359, 650, 969, 1386, 1502, 1923
HpySE526I	ACGT	3 cut(s)	1348, 1578, 1797
Hsp92I	GRCGYC	3 cut(s)	262, 970, 1797
HspAI	GCGC	3 cut(s)	513, 1455, 1926
LmnI	GCTCC	3 cut(s)	332, 350, 1493
Lsp1109I	GCAGC	5 cut(s)	710, 950, 1084, 1999, 2261
LweI	GCATC	3 cut(s)	528, 859, 1084
MaeI	CTAG	3 cut(s)	953, 1211, 1611
MaeII	ACGT	3 cut(s)	1348, 1578, 1797
MaeIII	GTNAC	3 cut(s)	1233, 1271, 1397
MboII	GAAGA	9 cut(s)	43, 373, 423, 699, 1217, 1327, 1363, 1598, 2257
MflI	RGATCY	3 cut(s)	371, 699, 2262
MhlI	GDGCHC	2 cut(s)	21, 1086
MluCI	AATT	8 cut(s)	163, 355, 398, 449, 1152, 1264, 2125, 2221
MlyI	GAGTC	3 cut(s)	1295, 1373, 1460
MmeI	TCCRAC	5 cut(s)	37, 192, 310, 657, 1335
MspA1I	CMGCKG	1 cut(s)	188
MspI	CCGG	8 cut(s)	137, 1317, 1382, 1390, 1525, 1725, 1856, 1892
MspR9I	CCNGG	4 cut(s)	770, 812, 1343, 1382
MvaI	CCWGG	3 cut(s)	770, 812, 1343
MvnI	CGCG	1 cut(s)	1505
MwoI	GCNNNNNNNGC	7 cut(s)	185, 359, 650, 969, 1386, 1502, 1923
NciI	CCSGG	1 cut(s)	1382
NcoI	CCATGG	1 cut(s)	2110
NdeI	CATATG	3 cut(s)	505, 883, 2023
NlaIV	GGNNCC	6 cut(s)	6, 701, 714, 1495, 1616, 1854
NmeAIII	GCCGAG	1 cut(s)	699
NmuCI	GTSAC	2 cut(s)	1233, 1397
NruI	TCGCGA	1 cut(s)	1505
NspI	RCATGY	2 cut(s)	1744, 2103
NspV	TTCGAA	2 cut(s)	405, 2294
PagI	TCATGA	1 cut(s)	267
PciI	ACATGT	1 cut(s)	1740
PcsI	WCGNNNNNNNCGW	1 cut(s)	1514
PfeI	GAWTC	7 cut(s)	112, 158, 323, 623, 980, 1190, 1630
PflFI	GACNNNGTC	1 cut(s)	1799
PflMI	CCANNNNNTGG	1 cut(s)	619
PinAI	ACCGGT	3 cut(s)	1316, 1855, 1891
PkrI	GCNGC	7 cut(s)	725, 965, 1099, 1406, 2014, 2108, 2251
PleI	GAGTC	3 cut(s)	1295, 1372, 1460
PpsI	GAGTC	3 cut(s)	1295, 1372, 1460
PpuMI	RGGWCCY	1 cut(s)	1614
PscI	ACATGT	1 cut(s)	1740
Psp1406I	AACGTT	1 cut(s)	1578
Psp5II	RGGWCCY	1 cut(s)	1614
Psp6I	CCWGG	3 cut(s)	768, 810, 1341
PspFI	CCCAGC	3 cut(s)	456, 1071, 1279
PspGI	CCWGG	3 cut(s)	768, 810, 1341
PspN4I	GGNNCC	6 cut(s)	6, 701, 714, 1495, 1616, 1854
PspPPI	RGGWCCY	1 cut(s)	1614
PsrI	GAACNNNNNNTAC	4 cut(s)	465, 497, 1577, 1609
PstI	CTGCAG	2 cut(s)	1246, 1333
PsuI	RGATCY	3 cut(s)	371, 699, 2262
PsyI	GACNNNGTC	1 cut(s)	1799
PvuII	CAGCTG	1 cut(s)	188
RruI	TCGCGA	1 cut(s)	1505
SatI	GCNGC	7 cut(s)	724, 964, 1098, 1405, 2013, 2107, 2250
SchI	GAGTC	3 cut(s)	1295, 1373, 1460
ScrFI	CCNGG	4 cut(s)	770, 812, 1343, 1382
SduI	GDGCHC	2 cut(s)	21, 1086
SfaNI	GCATC	3 cut(s)	528, 859, 1084
SfcI	CTRYAG	3 cut(s)	895, 1242, 1329
SfiI	GGCCNNNNNGGCC	1 cut(s)	1386
SfuI	TTCGAA	2 cut(s)	405, 2294
SgrAI	CRCCGGYG	1 cut(s)	1316
SinI	GGWCC	9 cut(s)	4, 610, 713, 772, 1614, 1853, 1882, 1912, 1984
SmlI	CTYRAG	2 cut(s)	830, 2244
SmoI	CTYRAG	2 cut(s)	830, 2244
Sse9I	AATT	8 cut(s)	163, 355, 398, 449, 1152, 1264, 2125, 2221
SspI	AATATT	2 cut(s)	693, 2181
SspMI	CTAG	3 cut(s)	953, 1211, 1611
StyD4I	CCNGG	4 cut(s)	768, 810, 1341, 1380
StyI	CCWWGG	5 cut(s)	973, 1435, 1610, 2110, 2140
TaaI	ACNGT	7 cut(s)	441, 607, 1079, 1239, 1397, 1817, 1964
TaiI	ACGT	3 cut(s)	1351, 1581, 1800
TasI	AATT	8 cut(s)	163, 355, 398, 449, 1152, 1264, 2125, 2221
TatI	WGTACW	8 cut(s)	76, 552, 1062, 1584, 1689, 1777, 1810, 2121
TauI	GCSGC	2 cut(s)	1407, 2109
TfiI	GAWTC	7 cut(s)	112, 158, 323, 623, 980, 1190, 1630
TscAI	CASTG	4 cut(s)	26, 1030, 1168, 1242
TseFI	GTSAC	2 cut(s)	1233, 1397
TseI	GCWGC	5 cut(s)	723, 963, 1097, 2012, 2249
Tsp45I	GTSAC	2 cut(s)	1233, 1397
TspDTI	ATGAA	6 cut(s)	171, 572, 1257, 1956, 2156, 2277
TspGWI	ACGGA	4 cut(s)	332, 408, 1506, 1735
TspRI	CASTG	4 cut(s)	26, 1030, 1168, 1242
Tth111I	GACNNNGTC	1 cut(s)	1799
Van91I	CCANNNNNTGG	1 cut(s)	619
VneI	GTGCAC	1 cut(s)	1082
VpaK11BI	GGWCC	9 cut(s)	4, 610, 713, 772, 1614, 1853, 1882, 1912, 1984
XagI	CCTNNNNNAGG	1 cut(s)	12
XapI	RAATTY	2 cut(s)	163, 355
XceI	RCATGY	2 cut(s)	1744, 2103
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1382
XmaJI	CCTAGG	1 cut(s)	1610
XmiI	GTMKAC	1 cut(s)	1867
XspI	CTAG	3 cut(s)	953, 1211, 1611
ZraI	GACGTC	1 cut(s)	1798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.