FvH4_4g26010

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003753
Physical Location & Seq
Forward (+)
27592358 .. 27594921
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26010.t1

Sequence Viewer

Length: 1209 bp
ATGGACCCTGTTAGGAAGAACTGGGGATTGAAGAGGAGTGCGGAAGCAGTAGCAGTTGGAGTTGTTTTGGTCGGTGTACTTTCACTTTTTTTGGGCCTCAGACCCCAAAAGATTCATTCTAGTGGTTGTCACTTCCCGGCAATCTATAACTTTGGTGATTCAAATTCCGACACAGGTGCTTTATCAGCTGTGTTTTATCGGCTTCCTTCTCCCTATGGCAATACATTCTTTGGCAAACCTTCGGGAAGAATCTCTGATGGGCGTCTCATGATCGATTTTATAGCTCAGAAGTTGGGTTTGCCATTTTTGAGTGCGTATCTTGATTCTGTTGGAGCAAACTTCCGTCATGGAGCAAATTTTGCAATTGGAGGATCTACAATCTTGCCATTAGATGGAAGGATGTTTGAAGGAAGATTTAGTCCCATCTCTCTTGACATACAATTTAGGCAGTTTGCGCAGCTTAAAGCTCGTGTAAAAGAGGTTATTAGTCAAGATAAAAGCTCATATGTCAGCGCCACCATCCCCAGACCAGAGGACTTCTCGAAGGCATTGTACACCGTAGACATAGGACAAAACGATATTCATGCTGGATTAAGGTGGAAGACGGTAGACCAAGTGCTGGCATCCATTTCTGACATGACTGCTAAGTTAGCTTTGACAATAGAGCAATTATACCAACAAGGTGGTAGAATATTTTGGATCCATAACACAGGTCCACTCGGTTGCTTGCCTCTGGTTCTTGCACATACACAGCCAGAACCTAGTGACCTGGACCAGAGTGACTGTTTGAAGTCTTACAATGAGGTGGCGCAAGAGTTCAACAGACAGCTCAAGGAGAAAGTACTTGATCTACTAGCCAAGCTCTCGGATGCAGTTCTTACTTATGTTGATATCTATACAGCAAAATACACACTTATTAGTGAAGCAGAGAAGTATGGTTTTATTGATCCTGTAGGTCAATGCTGCGGAAGCCAAGGTGATTCTCCTCACCATTGCGGGTTGAATGCTACTACTGCCAATGGCACTGAAGTATTTGGTGGCTCCTGTAGCAATCCTTCAAAGTACATTAGCTGGGATAGTGTGCACTATTCGGATGCTGCTAACGAATGGGTAGCCAAACGAGTTATAGAAGGCTCATTCTCAGACCCTCCAGTTTCGATCACTGAAGCATGTCGAAAACATCCATACTTCGATTCCTCCCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

403

Amino Acids

44.08

Weight (kDa)

6.37

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 48 - 375 4.2e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
AccB7I CCANNNNNTGG 2 cut(s) 392, 619
AccI GTMKAC 2 cut(s) 561, 609
AciI CCGC 3 cut(s) 41, 966, 996
AclWI GGATC 4 cut(s) 379, 694, 707, 941
AcsI RAATTY 2 cut(s) 163, 355
AcuI CTGAAG 2 cut(s) 1047, 1185
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 4 cut(s) 78, 554, 843, 1064
AfiI CCNNNNNNNGG 2 cut(s) 392, 619
AgsI TTSAA 7 cut(s) 31, 162, 407, 790, 820, 1003, 1059
AjnI CCWGG 1 cut(s) 768
AluBI AGCT 9 cut(s) 188, 284, 460, 467, 501, 653, 829, 862, 1071
AluI AGCT 9 cut(s) 188, 284, 460, 467, 501, 653, 829, 862, 1071
Alw21I GWGCWC 1 cut(s) 1086
Alw26I GTCTC 1 cut(s) 269
Alw44I GTGCAC 1 cut(s) 1082
AlwI GGATC 4 cut(s) 379, 694, 707, 941
AoxI GGCC 1 cut(s) 94
ApaLI GTGCAC 1 cut(s) 1082
ApeKI GCWGC 3 cut(s) 457, 963, 1097
ApoI RAATTY 2 cut(s) 163, 355
AspLEI GCGC 3 cut(s) 457, 515, 811
AspS9I GGNCC 4 cut(s) 4, 94, 713, 772
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 167, 980, 989
AvaII GGWCC 3 cut(s) 4, 713, 772
BaeGI GKGCMC 1 cut(s) 1086
BamHI GGATCC 1 cut(s) 699
BarI GAAGNNNNNNTAC 4 cut(s) 536, 568, 1039, 1071
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 608
Bbv12I GWGCWC 1 cut(s) 1086
BbvI GCAGC 3 cut(s) 469, 950, 1084
BccI CCATC 4 cut(s) 251, 386, 431, 527
BcgI CGANNNNNNTGC 4 cut(s) 158, 192, 566, 600
BciT130I CCWGG 1 cut(s) 770
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 3 cut(s) 120, 762, 854
BfmI CTRYAG 2 cut(s) 951, 1045
BfoI RGCGCY 1 cut(s) 516
BisI GCNGC 3 cut(s) 458, 964, 1098
BlsI GCNGC 3 cut(s) 459, 965, 1099
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 2 cut(s) 137, 770
Bme18I GGWCC 3 cut(s) 4, 713, 772
BmgT120I GGNCC 4 cut(s) 4, 94, 713, 772
BmiI GGNNCC 3 cut(s) 6, 701, 1042
BmrFI CCNGG 2 cut(s) 137, 770
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 3 cut(s) 632, 859, 1084
BmuI ACTGGG 1 cut(s) 31
BpiI GAAGAC 1 cut(s) 608
BplI GAGNNNNNCTC 2 cut(s) 524, 556
BpmI CTGGAG 1 cut(s) 1134
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 1 cut(s) 137
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 973
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 619
Bse1I ACTGG 2 cut(s) 26, 1151
Bse3DI GCAATG 1 cut(s) 991
BseBI CCWGG 1 cut(s) 770
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 973
BseGI GGATG 6 cut(s) 405, 519, 623, 874, 1099, 1180
BseLI CCNNNNNNNGG 2 cut(s) 392, 619
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 3 cut(s) 112, 299, 1155
BseNI ACTGG 2 cut(s) 26, 1151
BseRI GAGGAG 2 cut(s) 49, 975
BseSI GKGCMC 1 cut(s) 1086
BseXI GCAGC 3 cut(s) 469, 950, 1084
BseYI CCCAGC 1 cut(s) 1071
BshFI GGCC 1 cut(s) 96
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 1086
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 392, 619
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 405
BsmI GAATGC 1 cut(s) 1009
BsnI GGCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 1086
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
BspACI CCGC 3 cut(s) 41, 966, 996
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 3 cut(s) 111, 298, 1154
BspDI ATCGAT 1 cut(s) 273
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 3 cut(s) 6, 701, 1042
BspPI GGATC 4 cut(s) 379, 694, 707, 941
BsrDI GCAATG 1 cut(s) 991
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 2 cut(s) 26, 1151
BssECI CCNNGG 1 cut(s) 973
BssMI GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
BssNI GRCGYC 1 cut(s) 262
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 973
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 3 cut(s) 559, 607, 785
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 262
BstAPI GCANNNNNTGC 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 621, 728
BstDEI CTNAG 4 cut(s) 98, 285, 645, 1141
BstF5I GGATG 6 cut(s) 405, 519, 623, 874, 1099, 1180
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 3 cut(s) 457, 515, 811
BstKTI GATC 6 cut(s) 273, 374, 702, 850, 949, 1161
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
BstMWI GCNNNNNNNGC 7 cut(s) 185, 359, 454, 650, 969, 1013, 1047
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 2 cut(s) 135, 768
BstSFI CTRYAG 2 cut(s) 951, 1045
BstSLI GKGCMC 1 cut(s) 1086
BstV1I GCAGC 3 cut(s) 469, 950, 1084
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 2 cut(s) 371, 699
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 2 cut(s) 371, 699
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 6 cut(s) 405, 519, 623, 874, 1099, 1180
BtsIMutI CAGTG 2 cut(s) 1023, 1161
Cac8I GCNNGC 2 cut(s) 621, 728
CciI TCATGA 1 cut(s) 267
CfoI GCGC 3 cut(s) 457, 515, 811
Cfr13I GGNCC 4 cut(s) 4, 94, 713, 772
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 1 cut(s) 251
Csp6I GTAC 4 cut(s) 77, 553, 842, 1063
CviAII CATG 5 cut(s) 268, 347, 584, 637, 1170
CviQI GTAC 4 cut(s) 77, 553, 842, 1063
DdeI CTNAG 4 cut(s) 98, 285, 645, 1141
DpnI GATC 6 cut(s) 272, 373, 701, 849, 948, 1160
DpnII GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 973
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 3 cut(s) 4, 713, 772
Eco57I CTGAAG 2 cut(s) 1047, 1185
EcoRII CCWGG 1 cut(s) 768
EcoRV GATATC 1 cut(s) 892
EcoT14I CCWWGG 1 cut(s) 973
ErhI CCWWGG 1 cut(s) 973
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 5 cut(s) 271, 350, 587, 640, 1173
FaqI GGGAC 1 cut(s) 405
FatI CATG 5 cut(s) 267, 346, 583, 636, 1169
FauI CCCGC 1 cut(s) 989
FauNDI CATATG 1 cut(s) 505
FblI GTMKAC 2 cut(s) 561, 609
Fnu4HI GCNGC 3 cut(s) 458, 964, 1098
FokI GGATG 6 cut(s) 412, 506, 610, 881, 1106, 1167
Fsp4HI GCNGC 3 cut(s) 458, 964, 1098
FspBI CTAG 3 cut(s) 120, 762, 854
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 3 cut(s) 456, 514, 810
GluI GCNGC 3 cut(s) 458, 964, 1098
GsaI CCCAGC 1 cut(s) 1075
GsuI CTGGAG 1 cut(s) 1134
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 251
HhaI GCGC 3 cut(s) 457, 515, 811
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 5 cut(s) 271, 350, 587, 640, 1173
Hin6I GCGC 3 cut(s) 455, 513, 809
HinP1I GCGC 3 cut(s) 455, 513, 809
HinfI GANTC 6 cut(s) 112, 158, 249, 323, 980, 1193
HpaII CCGG 1 cut(s) 137
HphI GGTGA 3 cut(s) 167, 980, 989
Hpy166II GTNNAC 6 cut(s) 77, 555, 562, 610, 716, 1084
Hpy188I TCNGA 8 cut(s) 101, 169, 256, 288, 634, 868, 1093, 1144
Hpy188III TCNNGA 6 cut(s) 243, 268, 320, 431, 491, 541
Hpy8I GTNNAC 6 cut(s) 77, 555, 562, 610, 716, 1084
HpyAV CCTTC 7 cut(s) 216, 249, 390, 401, 538, 1065, 1124
HpyCH4III ACNGT 3 cut(s) 559, 607, 785
HpyCH4V TGCA 4 cut(s) 362, 743, 872, 1084
HpyF10VI GCNNNNNNNGC 7 cut(s) 185, 359, 454, 650, 969, 1013, 1047
HpyF3I CTNAG 4 cut(s) 98, 285, 645, 1141
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 5 cut(s) 271, 350, 587, 640, 1173
HspAI GCGC 3 cut(s) 455, 513, 809
Kzo9I GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
LmnI GCTCC 3 cut(s) 332, 350, 1046
Lsp1109I GCAGC 3 cut(s) 469, 950, 1084
LweI GCATC 3 cut(s) 632, 859, 1084
MaeI CTAG 3 cut(s) 120, 762, 854
MaeIII GTNAC 3 cut(s) 128, 764, 779
MalI GATC 6 cut(s) 272, 373, 701, 849, 948, 1160
MboI GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
MboII GAAGA 5 cut(s) 28, 43, 258, 423, 613
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 2 cut(s) 371, 699
MhlI GDGCHC 1 cut(s) 1086
MluCI AATT 5 cut(s) 163, 355, 363, 440, 668
MmeI TCCRAC 3 cut(s) 37, 192, 310
MseI TTAA 2 cut(s) 462, 593
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 137, 770
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 1009
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 7 cut(s) 185, 359, 454, 650, 969, 1013, 1047
NciI CCSGG 1 cut(s) 137
NdeI CATATG 1 cut(s) 505
NdeII GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
NlaIII CATG 5 cut(s) 271, 350, 587, 640, 1173
NlaIV GGNNCC 3 cut(s) 6, 701, 1042
NmuCI GTSAC 3 cut(s) 128, 764, 779
NsbI TGCGCA 1 cut(s) 456
NspI RCATGY 1 cut(s) 1173
PagI TCATGA 1 cut(s) 267
PctI GAATGC 1 cut(s) 1009
PfeI GAWTC 6 cut(s) 112, 158, 249, 323, 980, 1193
PflMI CCANNNNNTGG 2 cut(s) 392, 619
PkrI GCNGC 3 cut(s) 459, 965, 1099
Psp6I CCWGG 1 cut(s) 768
PspFI CCCAGC 1 cut(s) 1071
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 3 cut(s) 6, 701, 1042
PspPI GGNCC 4 cut(s) 4, 94, 713, 772
PsuI RGATCY 2 cut(s) 371, 699
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 4 cut(s) 78, 554, 843, 1064
RsaNI GTAC 4 cut(s) 77, 553, 842, 1063
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 2 cut(s) 462, 593
SatI GCNGC 3 cut(s) 458, 964, 1098
Sau3AI GATC 6 cut(s) 270, 371, 699, 847, 946, 1158
Sau96I GGNCC 4 cut(s) 4, 94, 713, 772
ScaI AGTACT 1 cut(s) 843
ScrFI CCNGG 2 cut(s) 137, 770
SduI GDGCHC 1 cut(s) 1086
SfaNI GCATC 3 cut(s) 632, 859, 1084
SfcI CTRYAG 2 cut(s) 951, 1045
SinI GGWCC 3 cut(s) 4, 713, 772
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 5 cut(s) 163, 355, 363, 440, 668
SsiI CCGC 3 cut(s) 41, 966, 996
SspI AATATT 1 cut(s) 693
SspMI CTAG 3 cut(s) 120, 762, 854
StyD4I CCNGG 2 cut(s) 135, 768
StyI CCWWGG 1 cut(s) 973
TaaI ACNGT 3 cut(s) 559, 607, 785
TaqI TCGA 5 cut(s) 273, 542, 1157, 1174, 1191
TasI AATT 5 cut(s) 163, 355, 363, 440, 668
TatI WGTACW 4 cut(s) 76, 552, 841, 1062
TfiI GAWTC 6 cut(s) 112, 158, 249, 323, 980, 1193
Tru1I TTAA 2 cut(s) 462, 593
Tru9I TTAA 2 cut(s) 462, 593
TscAI CASTG 2 cut(s) 1030, 1168
TseFI GTSAC 3 cut(s) 128, 764, 779
TseI GCWGC 3 cut(s) 457, 963, 1097
Tsp45I GTSAC 3 cut(s) 128, 764, 779
TspDTI ATGAA 2 cut(s) 104, 572
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 1030, 1168
Van91I CCANNNNNTGG 2 cut(s) 392, 619
VneI GTGCAC 1 cut(s) 1082
VpaK11BI GGWCC 3 cut(s) 4, 713, 772
XapI RAATTY 2 cut(s) 163, 355
XceI RCATGY 1 cut(s) 1173
XmiI GTMKAC 2 cut(s) 561, 609
XspI CTAG 3 cut(s) 120, 762, 854
ZrmI AGTACT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.