MD13G1098700.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003753
Physical Location & Seq
Forward (+)
6990610 .. 6993352
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1098700.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGACCGTGCAAGTTTTGGTAGTATCAATAGGTGGTGGAGAGGCCCTTGGGGAGTTAAGAAGAGTGTGGAGGCAGTGGCAGTAGGAGTGGTTTTAGTTGGTGCCCTTTCGGTTTTCTTAGGCCTTGCACCACAAAACACATCAAGTAATGGTTGCCAATTCCCAGCAATGTTTAACTTTGGCGATTCTAATTCCGACACGGGTGGCTTATCAGCCACATTTTTTCGACTTCCAGCCCCCTATGGCAATACATTCTTTGGTAAACCTTCAGGCAGGTTTTCAGACGGGCGTCTCATGATCGATTTTATAGCTCAGAAGTTGGGGTTTCCATTTGTGAGTGCTTATCTGGATGCTGTTGGAACTAATTTTTATCATGGAGCAAGTTTTGCGACCGGAGGATCTACCATTCAGCCATTAGATGGCAAGATGTTTGAAGCGAGATATAGCCCCATCTCTCTTAACGTGCAGCTTGCCCAATTTGCGCAGTTGAAAGCGCGTGCGAATGAGGTTTTTGCTGAAGGTAAAAGCTCAAAGGTCAGGGCCAGCCTCCCTAGACCAAGCGACTTCTCCAAGGCTTTGTACACATTAGACATTGGACAAAATGATCTTCATGCTGGATTAAGATTGAAGACGCTGGACGAAGTGCTTGCATCCCTTTCCAATATCACTGCACAGTTTGCTTCGACAATAGAGCAACTCTACCAACAAGGAGCAAGAGTTTTTTGGATTCACAACACTGGTCCCCTTGGCTGCTTGCCTGCAAGTCTTGCATATAAACTGCCAAAACCCGGCGACCTGGACCAAAACGGTTGCTTGAAACCTCACAATGAGGTGGCTCAAGAATTTAACCGGAAGCTCAAGGAAAGAGTGCTTAGCCTAAGGACCCAACTTTCTGATGCAGTTCTCACTTATGTCGATATTTACACAGCAAAATACACGCTTATCAATGAGGCAGAGAAGTACGGTTTTTCTAGTCCTCTAGTTCAATGCTGTGGAAGTTACGGCGATTACCATGTCCATTGTGGGATGAAAGCTATTGTTAATGGCACTGAAGTTGAGGCTCTCTGTAGCAATCCTTCGAAGTACATTAGCTGGGATGGGGTGCACTATTCCGATGCTGCAAATGAATGGATAGCCTACCAAATTATGGACGGCTCATTCTCCGACCCTCCAGTATCAATCACTGAAGCGTGTCACAGTCCTCCACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

405

Amino Acids

44.06

Weight (kDa)

6.71

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 381 8.6e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
Acc36I ACCTGC 1 cut(s) 264
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 2 cut(s) 419, 1147
AccII CGCG 1 cut(s) 496
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 4 cut(s) 252, 537, 1071, 1206
AcyI GRCGYC 1 cut(s) 289
AfaI GTAC 3 cut(s) 581, 962, 1085
AfiI CCNNNNNNNGG 3 cut(s) 419, 788, 1147
AgsI TTSAA 5 cut(s) 434, 490, 628, 817, 986
AjnI CCWGG 1 cut(s) 795
AluBI AGCT 6 cut(s) 311, 469, 528, 856, 1034, 1092
AluI AGCT 6 cut(s) 311, 469, 528, 856, 1034, 1092
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 1 cut(s) 296
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 1 cut(s) 406
AoxI GGCC 3 cut(s) 43, 121, 540
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 3 cut(s) 466, 750, 1118
ApoI RAATTY 1 cut(s) 842
AspLEI GCGC 2 cut(s) 484, 496
AspS9I GGNCC 6 cut(s) 4, 44, 540, 740, 799, 882
AsuC2I CCSGG 1 cut(s) 789
AsuII TTCGAA 1 cut(s) 1079
AvaII GGWCC 4 cut(s) 4, 740, 799, 882
AxyI CCTNAGG 1 cut(s) 878
BaeGI GKGCMC 2 cut(s) 106, 1107
BanI GGYRCC 1 cut(s) 101
BarI GAAGNNNNNNTAC 2 cut(s) 1060, 1092
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 3 cut(s) 478, 737, 1105
BccI CCATC 3 cut(s) 413, 458, 1091
BceAI ACGGC 2 cut(s) 1018, 1168
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 1 cut(s) 797
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 3 cut(s) 552, 972, 980
BfmI CTRYAG 1 cut(s) 1066
BfuAI ACCTGC 1 cut(s) 264
BisI GCNGC 3 cut(s) 467, 751, 1119
BlpI GCTNAGC 1 cut(s) 872
BlsI GCNGC 3 cut(s) 468, 752, 1120
Bme1390I CCNGG 2 cut(s) 789, 797
Bme18I GGWCC 4 cut(s) 4, 740, 799, 882
BmgT120I GGNCC 6 cut(s) 4, 44, 540, 740, 799, 882
BmiI GGNNCC 3 cut(s) 103, 742, 884
BmrFI CCNGG 2 cut(s) 789, 797
BmsI GCATC 4 cut(s) 340, 659, 886, 1105
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 1155
Bpu1102I GCTNAGC 1 cut(s) 872
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 2 cut(s) 822, 842
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 47, 570, 745
BsaWI WCCGGW 2 cut(s) 392, 849
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 788, 1147
Bse1I ACTGG 2 cut(s) 742, 1172
Bse21I CCTNAGG 1 cut(s) 878
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 797
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 47, 570, 745
BseGI GGATG 4 cut(s) 355, 650, 1032, 1102
BseLI CCNNNNNNNGG 3 cut(s) 419, 788, 1147
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 2 cut(s) 742, 1172
BseSI GKGCMC 2 cut(s) 106, 1107
BseXI GCAGC 3 cut(s) 478, 737, 1105
BseYI CCCAGC 2 cut(s) 163, 1092
BsgI GTGCAG 2 cut(s) 485, 654
Bsh1236I CGCG 1 cut(s) 496
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 3 cut(s) 45, 123, 542
BshNI GGYRCC 1 cut(s) 101
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 1107
BsiSI CCGG 3 cut(s) 393, 789, 850
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 3 cut(s) 419, 788, 1147
BsmAI GTCTC 1 cut(s) 296
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 3 cut(s) 45, 123, 542
Bsp119I TTCGAA 1 cut(s) 1079
Bsp1286I GDGCHC 2 cut(s) 106, 1107
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 3 cut(s) 297, 398, 604
Bsp1720I GCTNAGC 1 cut(s) 872
BspANI GGCC 3 cut(s) 45, 123, 542
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 1 cut(s) 496
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 103, 742, 884
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 174
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 2 cut(s) 742, 1172
BssECI CCNNGG 3 cut(s) 47, 570, 745
BssMI GATC 3 cut(s) 297, 398, 604
BssNI GRCGYC 1 cut(s) 289
BssT1I CCWWGG 3 cut(s) 47, 570, 745
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 6 cut(s) 8, 675, 809, 965, 1199, 1209
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 289
BstAPI GCANNNNNTGC 3 cut(s) 386, 677, 767
BstAUI TGTACA 1 cut(s) 579
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 6 cut(s) 471, 498, 544, 648, 755, 759
BstDEI CTNAG 4 cut(s) 118, 312, 872, 878
BstF5I GGATG 4 cut(s) 355, 650, 1032, 1102
BstFNI CGCG 1 cut(s) 496
BstHHI GCGC 2 cut(s) 484, 496
BstKTI GATC 3 cut(s) 300, 401, 607
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 297, 398, 604
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 386, 479, 677, 767
BstNI CCWGG 1 cut(s) 797
BstPAI GACNNNNGTC 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 787, 795
BstSFI CTRYAG 1 cut(s) 1066
BstSLI GKGCMC 2 cut(s) 106, 1107
BstUI CGCG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 478, 737, 1105
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 878
BsuRI GGCC 3 cut(s) 45, 123, 542
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 4 cut(s) 355, 650, 1032, 1102
BtsI GCAGTG 2 cut(s) 81, 666
BtsIMutI CAGTG 5 cut(s) 81, 666, 735, 1047, 1182
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 6 cut(s) 471, 498, 544, 648, 755, 759
CciI TCATGA 1 cut(s) 294
CfoI GCGC 2 cut(s) 484, 496
Cfr13I GGNCC 6 cut(s) 4, 44, 540, 740, 799, 882
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 2 cut(s) 278, 640
Csp6I GTAC 3 cut(s) 580, 961, 1084
CviAII CATG 4 cut(s) 295, 374, 611, 1013
CviQI GTAC 3 cut(s) 580, 961, 1084
DdeI CTNAG 4 cut(s) 118, 312, 872, 878
DpnI GATC 3 cut(s) 299, 400, 606
DpnII GATC 3 cut(s) 297, 398, 604
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 3 cut(s) 47, 570, 745
Eco147I AGGCCT 1 cut(s) 123
Eco47I GGWCC 4 cut(s) 4, 740, 799, 882
Eco57I CTGAAG 4 cut(s) 252, 537, 1071, 1206
Eco81I CCTNAGG 1 cut(s) 878
EcoO109I RGGNCCY 2 cut(s) 44, 882
EcoRII CCWGG 1 cut(s) 795
EcoT14I CCWWGG 3 cut(s) 47, 570, 745
ErhI CCWWGG 3 cut(s) 47, 570, 745
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 4 cut(s) 298, 377, 614, 1016
FaqI GGGAC 1 cut(s) 726
FatI CATG 4 cut(s) 294, 373, 610, 1012
Fnu4HI GCNGC 3 cut(s) 467, 751, 1119
FokI GGATG 4 cut(s) 362, 637, 1039, 1109
Fsp4HI GCNGC 3 cut(s) 467, 751, 1119
FspBI CTAG 3 cut(s) 552, 972, 980
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 2 cut(s) 483, 495
GluI GCNGC 3 cut(s) 467, 751, 1119
GsaI CCCAGC 2 cut(s) 167, 1096
GsuI CTGGAG 1 cut(s) 1155
HaeIII GGCC 3 cut(s) 45, 123, 542
HapII CCGG 3 cut(s) 393, 789, 850
HgaI GACGC 2 cut(s) 278, 640
HhaI GCGC 2 cut(s) 484, 496
Hin1I GRCGYC 1 cut(s) 289
Hin1II CATG 4 cut(s) 298, 377, 614, 1016
Hin6I GCGC 2 cut(s) 482, 494
HinP1I GCGC 2 cut(s) 482, 494
HinfI GANTC 2 cut(s) 185, 727
HpaII CCGG 3 cut(s) 393, 789, 850
Hpy166II GTNNAC 3 cut(s) 263, 582, 1105
Hpy188I TCNGA 7 cut(s) 196, 283, 315, 895, 1114, 1165, 1214
Hpy188III TCNNGA 3 cut(s) 295, 347, 839
Hpy8I GTNNAC 3 cut(s) 263, 582, 1105
HpyAV CCTTC 3 cut(s) 276, 512, 1086
HpyCH4III ACNGT 6 cut(s) 8, 675, 809, 965, 1199, 1209
HpyCH4IV ACGT 1 cut(s) 462
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 479, 677, 767
HpyF3I CTNAG 4 cut(s) 118, 312, 872, 878
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 289
Hsp92II CATG 4 cut(s) 298, 377, 614, 1016
HspAI GCGC 2 cut(s) 482, 494
Kzo9I GATC 3 cut(s) 297, 398, 604
LmnI GCTCC 2 cut(s) 377, 710
Lsp1109I GCAGC 3 cut(s) 478, 737, 1105
LweI GCATC 4 cut(s) 340, 659, 886, 1105
MaeI CTAG 3 cut(s) 552, 972, 980
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 998, 1193
MalI GATC 3 cut(s) 299, 400, 606
MboI GATC 3 cut(s) 297, 398, 604
MboII GAAGA 3 cut(s) 73, 599, 640
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 2 cut(s) 106, 1107
MluCI AATT 6 cut(s) 158, 190, 364, 476, 842, 1143
MmeI TCCRAC 3 cut(s) 219, 337, 1188
MseI TTAA 6 cut(s) 57, 174, 459, 620, 846, 1041
MspI CCGG 3 cut(s) 393, 789, 850
MspR9I CCNGG 2 cut(s) 789, 797
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 386, 479, 677, 767
NciI CCSGG 1 cut(s) 789
NdeII GATC 3 cut(s) 297, 398, 604
NlaIII CATG 4 cut(s) 298, 377, 614, 1016
NlaIV GGNNCC 3 cut(s) 103, 742, 884
NmuCI GTSAC 1 cut(s) 1193
NsbI TGCGCA 1 cut(s) 483
NspV TTCGAA 1 cut(s) 1079
PagI TCATGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 123
PfeI GAWTC 2 cut(s) 185, 727
PflMI CCANNNNNTGG 2 cut(s) 419, 1147
PkrI GCNGC 3 cut(s) 468, 752, 1120
PpuMI RGGWCCY 1 cut(s) 882
PshAI GACNNNNGTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 882
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 2 cut(s) 163, 1092
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 3 cut(s) 103, 742, 884
PspPI GGNCC 6 cut(s) 4, 44, 540, 740, 799, 882
PspPPI RGGWCCY 1 cut(s) 882
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 3 cut(s) 581, 962, 1085
RsaNI GTAC 3 cut(s) 580, 961, 1084
SaqAI TTAA 6 cut(s) 57, 174, 459, 620, 846, 1041
SatI GCNGC 3 cut(s) 467, 751, 1119
Sau3AI GATC 3 cut(s) 297, 398, 604
Sau96I GGNCC 6 cut(s) 4, 44, 540, 740, 799, 882
ScrFI CCNGG 2 cut(s) 789, 797
SduI GDGCHC 2 cut(s) 106, 1107
SfaNI GCATC 4 cut(s) 340, 659, 886, 1105
SfcI CTRYAG 1 cut(s) 1066
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 4 cut(s) 4, 740, 799, 882
SmlI CTYRAG 2 cut(s) 837, 857
SmoI CTYRAG 2 cut(s) 837, 857
Sse9I AATT 6 cut(s) 158, 190, 364, 476, 842, 1143
SseBI AGGCCT 1 cut(s) 123
SspMI CTAG 3 cut(s) 552, 972, 980
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 787, 795
StyI CCWWGG 3 cut(s) 47, 570, 745
TaaI ACNGT 6 cut(s) 8, 675, 809, 965, 1199, 1209
TaiI ACGT 1 cut(s) 465
TaqI TCGA 5 cut(s) 226, 300, 683, 915, 1079
TasI AATT 6 cut(s) 158, 190, 364, 476, 842, 1143
TatI WGTACW 2 cut(s) 579, 1083
TfiI GAWTC 2 cut(s) 185, 727
Tru1I TTAA 6 cut(s) 57, 174, 459, 620, 846, 1041
Tru9I TTAA 6 cut(s) 57, 174, 459, 620, 846, 1041
TscAI CASTG 5 cut(s) 81, 673, 742, 1054, 1189
TseFI GTSAC 1 cut(s) 1193
TseI GCWGC 3 cut(s) 466, 750, 1118
Tsp45I GTSAC 1 cut(s) 1193
TspDTI ATGAA 3 cut(s) 599, 1043, 1140
TspRI CASTG 5 cut(s) 81, 673, 742, 1054, 1189
Van91I CCANNNNNTGG 2 cut(s) 419, 1147
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 4 cut(s) 4, 740, 799, 882
XapI RAATTY 1 cut(s) 842
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XspI CTAG 3 cut(s) 552, 972, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.