MD13G1098900.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003753
Physical Location & Seq
Forward (+)
6995915 .. 6996992
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1098900.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTATAACTTTGGGGATTCAAATTCGGACACAGGTAGTTTCTCAGCTACATTTTATCAGCTCCCTTCTCCCTACGGCTACACGTACTTTGGTAACCTTCAGGCAGCTCAAAGGTTGGATTTTCCATTTTTAAGTGCTTATCTGGATTCTGTTGGAGCAAAGAATTTCGCAATTGGAGCATCTACAATTCAGCCATTAGATGCCAGGATATTTGAAGAGAGATATAGCTCCATCTCTTTTAACGTACAACTTTCACAATTTGCACAGCTCAAAGCACGCATAAATGAGCTTTTCCCTCGAGATAAAAGGTTCCATGTCAAGGCCGGCCTCCCAAGCCCAGAGGACTTCTCCAAGGTCTTGTATACTTTAGACATCAGACAAAACGGTCTTAATGCCGGGTTAAACTGGAAACCGGCAGACCAAGTGCTGGCATCCGTCTCCACCATAGCAGCGCAGTTAGCCTTGACAACTGAGATTGTTTTGTATGTGCAGCAACTCTACCAACAAGGGGCAAGAGTTTTTTGGATTCACAAATCACAACACAGGTCCGCTCGGGTGCTTGCCTTCAACTCTTGCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

194

Amino Acids

21.68

Weight (kDa)

8.99

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 2 - 178 1e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 551
AccI GTMKAC 1 cut(s) 362
AciI CCGC 1 cut(s) 549
AcsI RAATTY 2 cut(s) 22, 163
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 86, 246
AfiI CCNNNNNNNGG 3 cut(s) 319, 427, 508
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 21, 215, 568
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 6 cut(s) 47, 61, 107, 228, 268, 289
AluI AGCT 6 cut(s) 47, 61, 107, 228, 268, 289
Alw26I GTCTC 1 cut(s) 442
Ama87I CYCGRG 2 cut(s) 297, 552
AoxI GGCC 2 cut(s) 321, 325
ApeKI GCWGC 3 cut(s) 104, 449, 490
ApoI RAATTY 2 cut(s) 22, 163
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 546
AsuC2I CCSGG 1 cut(s) 397
AvaI CYCGRG 2 cut(s) 297, 552
AvaII GGWCC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 116, 461, 502
BccI CCATC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 205
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 442
BisI GCNGC 3 cut(s) 105, 450, 491
BlsI GCNGC 3 cut(s) 106, 451, 492
Bme1390I CCNGG 2 cut(s) 205, 397
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 2 cut(s) 297, 552
BmgT120I GGNCC 1 cut(s) 546
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 2 cut(s) 205, 397
BmsI GCATC 3 cut(s) 188, 190, 440
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpuMI CCSGG 1 cut(s) 397
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 427, 508
Bse118I RCCGGY 2 cut(s) 323, 412
Bse1I ACTGG 1 cut(s) 410
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 3 cut(s) 319, 427, 508
BseMII CTCAG 2 cut(s) 57, 462
BseNI ACTGG 1 cut(s) 410
BseXI GCAGC 3 cut(s) 116, 461, 502
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 2 cut(s) 323, 327
BsiHKCI CYCGRG 2 cut(s) 297, 552
BsiSI CCGG 3 cut(s) 324, 396, 413
BslI CCNNNNNNNGG 3 cut(s) 319, 427, 508
BsmAI GTCTC 1 cut(s) 442
BsmBI CGTCTC 1 cut(s) 442
BsnI GGCC 2 cut(s) 323, 327
BsoBI CYCGRG 2 cut(s) 297, 552
BspACI CCGC 1 cut(s) 549
BspANI GGCC 2 cut(s) 323, 327
BspCNI CTCAG 2 cut(s) 56, 463
BspLI GGNNCC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 551
BsrFI RCCGGY 2 cut(s) 323, 412
BsrI ACTGG 1 cut(s) 410
BssAI RCCGGY 2 cut(s) 323, 412
BssECI CCNNGG 1 cut(s) 351
BssNAI GTATAC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 277, 325, 429, 561
BstDEI CTNAG 2 cut(s) 43, 471
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 454
BstMAI GTCTC 1 cut(s) 442
BstMWI GCNNNNNNNGC 3 cut(s) 176, 333, 458
BstNI CCWGG 1 cut(s) 205
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 203, 395
BstV1I GCAGC 3 cut(s) 116, 461, 502
BstZ17I GTATAC 1 cut(s) 363
BsuRI GGCC 2 cut(s) 323, 327
BtsCI GGATG 1 cut(s) 431
Cac8I GCNNGC 4 cut(s) 277, 325, 429, 561
CfoI GCGC 1 cut(s) 454
Cfr10I RCCGGY 2 cut(s) 323, 412
Cfr13I GGNCC 1 cut(s) 546
Csp6I GTAC 2 cut(s) 85, 245
CviAII CATG 1 cut(s) 314
CviQI GTAC 2 cut(s) 85, 245
DdeI CTNAG 2 cut(s) 43, 471
DrdI GACNNNNNNGTC 1 cut(s) 384
DseDI GACNNNNNNGTC 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 297, 552
Eco91I GGTNACC 1 cut(s) 92
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 442
FaeI CATG 1 cut(s) 317
FaiI YATR 8 cut(s) 6, 225, 281, 315, 363, 446, 486, 580
FatI CATG 1 cut(s) 313
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 3 cut(s) 105, 450, 491
FokI GGATG 1 cut(s) 418
FseI GGCCGGCC 1 cut(s) 327
Fsp4HI GCNGC 3 cut(s) 105, 450, 491
GlaI GCGC 1 cut(s) 453
GluI GCNGC 3 cut(s) 105, 450, 491
HaeIII GGCC 2 cut(s) 323, 327
HapII CCGG 3 cut(s) 324, 396, 413
HhaI GCGC 1 cut(s) 454
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HinfI GANTC 3 cut(s) 17, 146, 526
HpaII CCGG 3 cut(s) 324, 396, 413
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 2 cut(s) 28, 377
Hpy188III TCNNGA 2 cut(s) 143, 299
Hpy8I GTNNAC 1 cut(s) 363
HpyAV CCTTC 3 cut(s) 75, 107, 574
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 2 cut(s) 83, 243
HpyCH4V TGCA 2 cut(s) 263, 490
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 333, 458
HpyF3I CTNAG 2 cut(s) 43, 471
HpySE526I ACGT 2 cut(s) 83, 243
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 1 cut(s) 452
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
LmnI GCTCC 4 cut(s) 66, 155, 176, 233
Lsp1109I GCAGC 3 cut(s) 116, 461, 502
LweI GCATC 3 cut(s) 188, 190, 440
MaeII ACGT 2 cut(s) 83, 243
MaeIII GTNAC 1 cut(s) 92
MbiI CCGCTC 1 cut(s) 551
MboII GAAGA 1 cut(s) 227
MfeI CAATTG 1 cut(s) 171
MluCI AATT 5 cut(s) 22, 163, 171, 186, 257
MmeI TCCRAC 2 cut(s) 96, 133
MnlI CCTC 3 cut(s) 306, 334, 338
MroNI GCCGGC 1 cut(s) 323
MseI TTAA 4 cut(s) 131, 240, 390, 401
MspI CCGG 3 cut(s) 324, 396, 413
MspR9I CCNGG 2 cut(s) 205, 397
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 205
MwoI GCNNNNNNNGC 3 cut(s) 176, 333, 458
NaeI GCCGGC 1 cut(s) 325
NciI CCSGG 1 cut(s) 397
NgoMIV GCCGGC 1 cut(s) 323
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 325
PfeI GAWTC 3 cut(s) 17, 146, 526
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 3 cut(s) 106, 451, 492
Ppu21I YACGTR 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 203
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 546
RigI GGCCGGCC 1 cut(s) 327
RsaI GTAC 2 cut(s) 86, 246
RsaNI GTAC 2 cut(s) 85, 245
SaqAI TTAA 4 cut(s) 131, 240, 390, 401
SatI GCNGC 3 cut(s) 105, 450, 491
Sau96I GGNCC 1 cut(s) 546
ScrFI CCNGG 2 cut(s) 205, 397
SfaNI GCATC 3 cut(s) 188, 190, 440
Sfr274I CTCGAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 5 cut(s) 22, 163, 171, 186, 257
SsiI CCGC 1 cut(s) 549
StyD4I CCNGG 2 cut(s) 203, 395
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 2 cut(s) 86, 246
TaqI TCGA 1 cut(s) 298
TasI AATT 5 cut(s) 22, 163, 171, 186, 257
TfiI GAWTC 3 cut(s) 17, 146, 526
Tru1I TTAA 4 cut(s) 131, 240, 390, 401
Tru9I TTAA 4 cut(s) 131, 240, 390, 401
TseI GCWGC 3 cut(s) 104, 449, 490
TspGWI ACGGA 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 2 cut(s) 22, 163
XhoI CTCGAG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.