AT1G74875

F-box protein At1g67623

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006630
Physical Location & Seq
Reverse (-)
28128470 .. 28129745
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74875.1

Sequence Viewer

Length: 732 bp
ATGGTAAATTTAGGTGCTCTAAACGACGAAGACAAGCGGAAGTGTGCCAACATGTTTCAAATTCACCTCGTAATCAATGTATTATCTTCATCATTATCCGAGTTTCGCAATCTTCAATTAGTTTCGAAATCGTTTAAACGTATCAGCAACGATCGATACATTCTGCAGCGGCTTTCCCTCAATAAAATCCCTCTTTTACCATGGCGCAACCGTAAAAAATTTCACAATTTTTTCAAAAGATGTCGCAAGAGTGGAAACCTAGAGGCTATATACCGTAAAGGACTTGTCGATTACTTTCATCGGGATAGTCATGAACGTCAACGAGACAGAGGACTCAAACATATAGCAAAAACTGCAAACAATGGAAATCAAGAAGCACAATATGTTTATGGGTTGATCCTAATTTGTCTTGGTGGCAAAACAAAACAAAATGGTTTCAAGATCTTGTCTTCTCTACGAAAACCTTTGATGTTGTCAAATACGATCAAGGAGATGGAAAAACATCGGACACATATCAGAAATATGTGGTGGTGGGGCGAACCGATGATGAGACACCTACAAAAAAGCTATGTTCGTGAGAACTGCAATTGCGATGGTCGAACCGAGATCTTTCCCAACTATTGTTTTCGTCATCGTTTTGGTGAGGACGACGACATGCTTACTTCTTCCGCTTGTGAGATATGTTTGTGCCATCATGAAGTCAAACTTTTCTTTGCTAATATTAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

243

Amino Acids

28.87

Weight (kDa)

9.76

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 62 - 163 4.5e-27 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 37, 169, 669
AclWI GGATC 1 cut(s) 391
AcsI RAATTY 3 cut(s) 7, 60, 218
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 4 cut(s) 59, 116, 235, 439
AluBI AGCT 1 cut(s) 567
AluI AGCT 1 cut(s) 567
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 318, 544
AlwI GGATC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 3 cut(s) 7, 60, 218
AspLEI GCGC 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 56, 653
AsuII TTCGAA 1 cut(s) 125
BbsI GAAGAC 2 cut(s) 36, 441
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 178
BccI CCATC 3 cut(s) 487, 587, 699
BcoDI GTCTC 2 cut(s) 318, 544
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 164
BglII AGATCT 2 cut(s) 441, 606
BisI GCNGC 2 cut(s) 167, 170
BlsI GCNGC 2 cut(s) 168, 171
BpiI GAAGAC 2 cut(s) 36, 441
Bpu14I TTCGAA 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 178
Bsh1285I CGRYCG 1 cut(s) 154
BshVI ATCGAT 1 cut(s) 154
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 19
BsmAI GTCTC 2 cut(s) 318, 544
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 5 cut(s) 151, 396, 441, 483, 606
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 3 cut(s) 37, 169, 669
BspDI ATCGAT 1 cut(s) 154
BspHI TCATGA 2 cut(s) 310, 694
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 5 cut(s) 151, 396, 441, 483, 606
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 2 cut(s) 212, 275
BstAPI GCANNNNNTGC 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 125
BstDSI CCRYGG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 5 cut(s) 154, 399, 444, 486, 609
BstMAI GTCTC 2 cut(s) 318, 544
BstMBI GATC 5 cut(s) 151, 396, 441, 483, 606
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNSI RCATGY 2 cut(s) 55, 658
BstSFI CTRYAG 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 2 cut(s) 36, 441
BstX2I RGATCY 2 cut(s) 441, 606
BstYI RGATCY 2 cut(s) 441, 606
Bsu15I ATCGAT 1 cut(s) 154
BsuTUI ATCGAT 1 cut(s) 154
BtgI CCRYGG 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 606
CciI TCATGA 2 cut(s) 310, 694
CfoI GCGC 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 154
CviAII CATG 5 cut(s) 52, 201, 311, 655, 695
CviJI RGCY 3 cut(s) 172, 266, 567
CviKI_1 RGCY 3 cut(s) 172, 266, 567
DpnI GATC 5 cut(s) 153, 398, 443, 485, 608
DpnII GATC 5 cut(s) 151, 396, 441, 483, 606
DraI TTTAAA 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 5 cut(s) 55, 204, 314, 658, 698
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 5 cut(s) 51, 200, 310, 654, 694
Fnu4HI GCNGC 2 cut(s) 167, 170
Fsp4HI GCNGC 2 cut(s) 167, 170
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 167, 170
HhaI GCGC 1 cut(s) 207
Hin1II CATG 5 cut(s) 55, 204, 314, 658, 698
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 320
HindII GTYRAC 1 cut(s) 320
HinfI GANTC 1 cut(s) 333
HphI GGTGA 2 cut(s) 56, 653
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 100, 507, 518
Hpy188III TCNNGA 6 cut(s) 302, 311, 371, 439, 575, 695
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 2 cut(s) 29, 653
HpyCH4III ACNGT 2 cut(s) 212, 275
HpyCH4IV ACGT 2 cut(s) 139, 316
HpyCH4V TGCA 3 cut(s) 166, 356, 585
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpySE526I ACGT 2 cut(s) 139, 316
Hsp92II CATG 5 cut(s) 55, 204, 314, 658, 698
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 151, 396, 441, 483, 606
Lsp1109I GCAGC 1 cut(s) 178
MaeI CTAG 1 cut(s) 260
MaeII ACGT 2 cut(s) 139, 316
MalI GATC 5 cut(s) 153, 398, 443, 485, 608
MboI GATC 5 cut(s) 151, 396, 441, 483, 606
MboII GAAGA 5 cut(s) 41, 78, 104, 441, 657
MfeI CAATTG 1 cut(s) 586
MflI RGATCY 2 cut(s) 441, 606
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 7 cut(s) 7, 60, 116, 218, 226, 402, 586
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 6 cut(s) 77, 188, 201, 256, 323, 637
MseI TTAA 2 cut(s) 135, 723
MspA1I CMGCKG 1 cut(s) 169
MssI GTTTAAAC 1 cut(s) 136
MunI CAATTG 1 cut(s) 586
MwoI GCNNNNNNNGC 1 cut(s) 353
NcoI CCATGG 1 cut(s) 200
NdeII GATC 5 cut(s) 151, 396, 441, 483, 606
NlaIII CATG 5 cut(s) 55, 204, 314, 658, 698
NspI RCATGY 2 cut(s) 55, 658
NspV TTCGAA 1 cut(s) 125
PagI TCATGA 2 cut(s) 310, 694
PciI ACATGT 1 cut(s) 51
PkrI GCNGC 2 cut(s) 168, 171
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 1 cut(s) 327
PmeI GTTTAAAC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 327
PscI ACATGT 1 cut(s) 51
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 2 cut(s) 441, 606
PvuI CGATCG 1 cut(s) 154
SaqAI TTAA 2 cut(s) 135, 723
SatI GCNGC 2 cut(s) 167, 170
Sau3AI GATC 5 cut(s) 151, 396, 441, 483, 606
SchI GAGTC 1 cut(s) 327
SduI GDGCHC 1 cut(s) 19
SetI ASST 8 cut(s) 16, 69, 142, 261, 319, 466, 558, 569
SfcI CTRYAG 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 125
Sse9I AATT 7 cut(s) 7, 60, 116, 218, 226, 402, 586
SsiI CCGC 3 cut(s) 37, 169, 669
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 2 cut(s) 212, 275
TaiI ACGT 2 cut(s) 142, 319
TaqI TCGA 4 cut(s) 125, 154, 288, 598
TasI AATT 7 cut(s) 7, 60, 116, 218, 226, 402, 586
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 2 cut(s) 135, 723
Tru9I TTAA 2 cut(s) 135, 723
TseI GCWGC 1 cut(s) 166
TspDTI ATGAA 4 cut(s) 78, 287, 327, 711
XapI RAATTY 3 cut(s) 7, 60, 218
XceI RCATGY 2 cut(s) 55, 658
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.