Rroxscaffold_5G00364230

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006630
Physical Location & Seq
Forward (+)
45490116 .. 45496333
6218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364230.1

Sequence Viewer

Length: 414 bp
ATGCAGAGGCTCTGTATAGACAAGGAGTGCTTGATCTTTTTTAGCTTGAGGAAGCGCGATATGAATTCGAATCGGGATTCGAGTGTTTGGAGAAAAGCTGCAGCAAAAGGTCACGTAGAAGCAACGTATATTTATGGGGTATTCTTAACTTGTGTAGGAGGTGAGGCGAAGCAACAAGGTCTACAATCCTTGTGTAGTCTCAATGGTAAAGATTTAAGTGAATCAAGAGAAAGGTTTATGACTTACATTCAAAGTATGTGGGTGAATAATGTCCTAGTACGTAAAATACTTACCTACAATGATCGTGTTAAAGCATGCAGTCAATGCGGAAATTTGCAGAATAATTTCGTAACCAAACGAGGCTGGGACGATATTTACGACGAAGAGAGAGCAATGATGCAGACGTATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

137

Amino Acids

15.92

Weight (kDa)

8.94

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 27 - 83 6.4e-11 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 181
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 327
AcsI RAATTY 2 cut(s) 64, 331
AfaI GTAC 1 cut(s) 279
AgsI TTSAA 1 cut(s) 251
AluBI AGCT 2 cut(s) 45, 98
AluI AGCT 2 cut(s) 45, 98
Alw26I GTCTC 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 98, 101
ApoI RAATTY 2 cut(s) 64, 331
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 173, 274
AsuII TTCGAA 1 cut(s) 68
BbvI GCAGC 2 cut(s) 85, 113
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 99
BisI GCNGC 2 cut(s) 99, 102
BlsI GCNGC 2 cut(s) 100, 103
BmsI GCATC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 67
BsaAI YACGTR 2 cut(s) 115, 281
Bse3DI GCAATG 1 cut(s) 399
BseMI GCAATG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 85, 113
BseYI CCCAGC 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 68
Bsp143I GATC 2 cut(s) 33, 301
BspACI CCGC 1 cut(s) 327
BspFNI CGCG 1 cut(s) 57
BspMAI CTGCAG 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 399
BssMI GATC 2 cut(s) 33, 301
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 324
BstBAI YACGTR 2 cut(s) 115, 281
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 316
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 36, 304
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 2 cut(s) 33, 301
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 99
BstSNI TACGTA 1 cut(s) 281
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 85, 113
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 278
CspCI CAANNNNNGTGG 2 cut(s) 239, 274
CviAII CATG 1 cut(s) 315
CviJI RGCY 4 cut(s) 10, 45, 98, 363
CviKI_1 RGCY 4 cut(s) 10, 45, 98, 363
CviQI GTAC 1 cut(s) 278
DpnI GATC 2 cut(s) 35, 303
DpnII GATC 2 cut(s) 33, 301
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco105I TACGTA 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 64
FaeI CATG 1 cut(s) 318
FaiI YATR 7 cut(s) 17, 62, 129, 135, 239, 257, 316
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 1 cut(s) 380
FatI CATG 1 cut(s) 314
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 2 cut(s) 99, 102
Fsp4HI GCNGC 2 cut(s) 99, 102
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 56
GluI GCNGC 2 cut(s) 99, 102
GsaI CCCAGC 1 cut(s) 367
HhaI GCGC 1 cut(s) 57
Hin1II CATG 1 cut(s) 318
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HinfI GANTC 3 cut(s) 70, 77, 221
HphI GGTGA 2 cut(s) 173, 274
Hpy166II GTNNAC 1 cut(s) 182
Hpy188III TCNNGA 2 cut(s) 74, 225
Hpy8I GTNNAC 1 cut(s) 182
Hpy99I CGWCG 1 cut(s) 383
HpyCH4IV ACGT 4 cut(s) 114, 125, 280, 404
HpyCH4V TGCA 5 cut(s) 4, 101, 318, 337, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpySE526I ACGT 4 cut(s) 114, 125, 280, 404
Hsp92II CATG 1 cut(s) 318
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 33, 301
LpnPI CCDG 1 cut(s) 349
Lsp1109I GCAGC 2 cut(s) 85, 113
LweI GCATC 1 cut(s) 387
MaeI CTAG 1 cut(s) 275
MaeII ACGT 4 cut(s) 114, 125, 280, 404
MaeIII GTNAC 2 cut(s) 110, 349
MalI GATC 2 cut(s) 35, 303
MboI GATC 2 cut(s) 33, 301
MboII GAAGA 1 cut(s) 395
MluCI AATT 3 cut(s) 64, 331, 343
MnlI CCTC 4 cut(s) 42, 152, 157, 353
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 4 cut(s) 146, 215, 309, 412
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeII GATC 2 cut(s) 33, 301
NlaIII CATG 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 1 cut(s) 318
NspV TTCGAA 1 cut(s) 68
PaeI GCATGC 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 3 cut(s) 70, 77, 221
PkrI GCNGC 2 cut(s) 100, 103
Ppu21I YACGTR 2 cut(s) 115, 281
PspFI CCCAGC 1 cut(s) 363
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SaqAI TTAA 4 cut(s) 146, 215, 309, 412
SatI GCNGC 2 cut(s) 99, 102
Sau3AI GATC 2 cut(s) 33, 301
SfaNI GCATC 1 cut(s) 387
SfcI CTRYAG 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SnaBI TACGTA 1 cut(s) 281
SphI GCATGC 1 cut(s) 318
Sse9I AATT 3 cut(s) 64, 331, 343
SsiI CCGC 1 cut(s) 327
SspMI CTAG 1 cut(s) 275
TaiI ACGT 4 cut(s) 117, 128, 283, 407
TaqI TCGA 2 cut(s) 68, 80
TasI AATT 3 cut(s) 64, 331, 343
TfiI GAWTC 3 cut(s) 70, 77, 221
Tru1I TTAA 4 cut(s) 146, 215, 309, 412
Tru9I TTAA 4 cut(s) 146, 215, 309, 412
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 98, 101
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 77
XapI RAATTY 2 cut(s) 64, 331
XceI RCATGY 1 cut(s) 318
XmiI GTMKAC 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.