AT2G35280

DNA recombination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006630
Physical Location & Seq
Reverse (-)
14859378 .. 14860200
823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35280.1

Sequence Viewer

Length: 492 bp
ATGGATCCCAACAAAGTTGAAATTAGTCGTCTTGAGGCACTTCCTCAAGATCTACTCAGAGAAATAGTTGCCAAAATAGGGGTAAAATCCGCTGAAGATTATCACAATTGCATCCTTTCATGCAAAGAACTAGGTGCATCAGCAAACGATGAACGCGTGCTCAAAACCCTTAATCTTGCTCTGTTGGTGAAGAAACCGTTATCATGCCGCAAACACCTTCTTATTATGAAGAAATGTCTTGCCAACAACAACCCGGATGCTCACTACATAAAAGGTATAATATGGTACTTTAACCTTGATCATTGTGACGTTGATCTCCACCATATTGGCATAGCAGCTAACGGAGGCCAGAAAGAAGCAATCTACATGTATGCCATGCTATTATTATGTAGAGGAAGAACTGAAGAGGGAAAAACCTATATGAGCCAACTTGAATGGGCTAAGACACCACCATGGCTGAAACGTGTTGGAAACAAATCAAAACTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.52

Weight (kDa)

8.99

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 70 - 145 2.9e-14 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 156
AciI CCGC 2 cut(s) 90, 208
AclWI GGATC 1 cut(s) 12
AcuI CTGAAG 2 cut(s) 114, 423
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 78
AflIII ACRYGT 3 cut(s) 154, 366, 463
AgsI TTSAA 2 cut(s) 20, 434
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw21I GWGCWC 1 cut(s) 162
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 199
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 347
BclI TGATCA 1 cut(s) 298
BcnI CCSGG 1 cut(s) 254
BfaI CTAG 1 cut(s) 131
BglII AGATCT 1 cut(s) 49
BisI GCNGC 2 cut(s) 208, 336
BlsI GCNGC 2 cut(s) 209, 337
Bme1390I CCNGG 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 3 cut(s) 120, 146, 247
BpuEI CTTGAG 2 cut(s) 30, 53
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 452
Bsc4I CCNNNNNNNGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 2 cut(s) 111, 262
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 70
BseXI GCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 78
BsnI GGCC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 4, 49, 298, 313
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 2 cut(s) 90, 208
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 4 cut(s) 4, 49, 298, 313
BssT1I CCWWGG 1 cut(s) 452
Bst4CI ACNGT 1 cut(s) 198
Bst6I CTCTTC 1 cut(s) 399
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 56, 441
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 111, 262
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 4 cut(s) 7, 52, 301, 316
BstMBI GATC 4 cut(s) 4, 49, 298, 313
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 1 cut(s) 252
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 347
BstX2I RGATCY 2 cut(s) 4, 49
BstXI CCANNNNNNTGG 1 cut(s) 326
BstYI RGATCY 2 cut(s) 4, 49
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 2 cut(s) 111, 262
Cac8I GCNNGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 286
CviAII CATG 5 cut(s) 120, 204, 367, 376, 453
CviJI RGCY 5 cut(s) 338, 348, 426, 440, 457
CviKI_1 RGCY 5 cut(s) 338, 348, 426, 440, 457
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 2 cut(s) 56, 441
DpnI GATC 4 cut(s) 6, 51, 300, 315
DpnII GATC 4 cut(s) 4, 49, 298, 313
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 114, 423
EcoT14I CCWWGG 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 5 cut(s) 123, 207, 370, 379, 456
FatI CATG 5 cut(s) 119, 203, 366, 375, 452
FbaI TGATCA 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 208, 336
FokI GGATG 2 cut(s) 98, 269
Fsp4HI GCNGC 2 cut(s) 208, 336
FspBI CTAG 1 cut(s) 131
GluI GCNGC 2 cut(s) 208, 336
HaeIII GGCC 1 cut(s) 348
HapII CCGG 1 cut(s) 254
Hin1II CATG 5 cut(s) 123, 207, 370, 379, 456
HpaII CCGG 1 cut(s) 254
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 2 cut(s) 32, 47
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4IV ACGT 2 cut(s) 309, 463
HpyCH4V TGCA 3 cut(s) 111, 123, 137
HpyF3I CTNAG 2 cut(s) 56, 441
HpySE526I ACGT 2 cut(s) 309, 463
Hsp92II CATG 5 cut(s) 123, 207, 370, 379, 456
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 4, 49, 298, 313
LpnPI CCDG 2 cut(s) 267, 362
Lsp1109I GCAGC 1 cut(s) 347
LweI GCATC 3 cut(s) 120, 146, 247
MaeI CTAG 1 cut(s) 131
MaeII ACGT 2 cut(s) 309, 463
MaeIII GTNAC 1 cut(s) 305
MalI GATC 4 cut(s) 6, 51, 300, 315
MboI GATC 4 cut(s) 4, 49, 298, 313
MboII GAAGA 5 cut(s) 107, 202, 241, 408, 416
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 2 cut(s) 4, 49
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 2 cut(s) 21, 106
MluI ACGCGT 1 cut(s) 154
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 5 cut(s) 28, 54, 338, 386, 400
MseI TTAA 2 cut(s) 171, 291
MslI CAYNNNNRTG 1 cut(s) 451
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 254
MunI CAATTG 1 cut(s) 106
MvnI CGCG 1 cut(s) 156
NciI CCSGG 1 cut(s) 254
NcoI CCATGG 1 cut(s) 452
NdeII GATC 4 cut(s) 4, 49, 298, 313
NlaIII CATG 5 cut(s) 123, 207, 370, 379, 456
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 370
PciI ACATGT 1 cut(s) 366
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PkrI GCNGC 2 cut(s) 209, 337
PscI ACATGT 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 2 cut(s) 4, 49
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 451
SaqAI TTAA 2 cut(s) 171, 291
SatI GCNGC 2 cut(s) 208, 336
Sau3AI GATC 4 cut(s) 4, 49, 298, 313
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 1 cut(s) 162
SetI ASST 8 cut(s) 136, 219, 277, 297, 312, 340, 419, 466
SfaNI GCATC 3 cut(s) 120, 146, 247
SmiMI CAYNNNNRTG 1 cut(s) 451
SmlI CTYRAG 2 cut(s) 32, 45
SmoI CTYRAG 2 cut(s) 32, 45
Sse9I AATT 2 cut(s) 21, 106
SsiI CCGC 2 cut(s) 90, 208
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 1 cut(s) 198
TaiI ACGT 2 cut(s) 312, 466
TasI AATT 2 cut(s) 21, 106
TauI GCSGC 1 cut(s) 210
Tru1I TTAA 2 cut(s) 171, 291
Tru9I TTAA 2 cut(s) 171, 291
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 108, 165, 242
TspGWI ACGGA 1 cut(s) 357
XceI RCATGY 1 cut(s) 370
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.