AT2G16250

LRR receptor-like serine threonine-protein kinase At2g16250

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002531
Physical Location & Seq
Reverse (-)
7039682 .. 7043354
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16250.1

Sequence Viewer

Length: 2748 bp
ATGGTTGATCAAAGAAGAAGCGCATTGGGTTTTGTCTTGTTGTTGTTGTGTTTGGTACTGTTCTTCGACTGTGTTGTTGTTGGTCAAACACAGAGTCGATTCAGTGAGAAACTCATTTTGCTTAATCTCAGGTCTTCTTTAGGGTTAAGAGGAACAGATTGGCCAATTAAAGGTGACCCTTGTGTTGATTGGAGAGGTATACAATGTGAGAATGGTAGTATCATTGGGATCAATATCTCTGGGTTTAGGAGAACAAGAATTGGTAAGCTAAACCCACAATTCTCTGTTGATCCTCTTCGTAATCTAACTCGTTTGTCGTATTTTAACGCGTCCGGGTTAGCGTTACCGGGGACTATACCTGAGTGGTTTGGTGTTAGTTTGTTGGCATTGGAAGTATTGGATCTTAGTTCGTGTTCTGTTAACGGTGTTGTTCCTTTTACTCTTGGTAATCTCACTAGTTTAAGAACTTTGAATCTGTCTCAGAACAGTCTTACTAGTTTAGTTCCTAGCAGTTTAGGACAGTTGTTGAATCTATCACAGCTTGATCTCTCTAGGAACTCATTCACTGGTGTTCTTCCTCAATCTTTTAGCTCTCTGAAGAATCTGTTGACTCTTGATGTTTCTTCCAATTACCTTACTGGACCTATCCCTCCTGGTTTAGGAGCGTTATCAAAGCTTATCCATCTGAACTTTTCAAGCAATAGTTTCTCGTCTCCGATTCCTTCAGAGCTTGGTGATCTTGTCAATCTGGTTGACTTTGATCTCAGCATCAATTCATTGTCTGGTTCAGTTCCTCAGGAGCTAAGAAAGTTAAGTAAGTTGCAGCTAATGGCGATTGGCGATAACCTTTTGAGTGGTACTTTACCAGTAGATTTGTTTAGTGCTGAGAGCCAACTGCAAACTTTAGTTTTGAGGGAAAATGGTTTCTCTGGTAGTCTACCTGATGTGTGCTGGTCGTTGCCAAAATTGCGGATTCTTGACATTGCTAAGAACAACTTCACAGGACTGCTGCCATATTCTAGTTATGATTCAGACCAGATAGCTGAAATGGTTGATATCTCTTCGAACACATTCTATGGAGAGCTCACACCGATTCTTAGACGGTTCAGGATAATGGACCTCTCTGGTAATTATTTCGAAGGTAAGCTTCCTGATTATGTGACCGGGGAAAATGTGTCTGTTACAAGCAATTGCCTTCGAAATGAGAGGAGACAGAAGCCGTCAGCAATATGTGCAGCATTCTATAAGTCTAGAGGATTAGATTTTGATGATTTCGGACGTCCTAATTTGACACAACCGACATCGAAAAATGCTTCTTCAGGGATAAGCCGCAGAACAGTAATTATATTGGCAGCAGTGGGTGGTGGAGTTGCTTTTATTCTTCTTTTTGTTATCTTGCCAATTATTCTGGTTCTATGCATGCGCCATAGACGCAGAGCGGCACAAAGAGGAAATAACGACAGGCCAAAACCTGCCGGGGAAGCTTCACAACAACCACCTAAAGGAGCACAAACCTTTGATTTGTCACGTCTTGGAAATGCATTTTCTTATGAGCAGCTTCTTCAGGCAACAGAGGAATTCAATGATGCTAATCTGATAAAACGTGGTCATTCGGGGAATCTGTTTCGTGGTTTCTTGGAAAATGGTATACCCGTTGTTATAAAAAAGATCGATGTGCGGGAAGGTAAAAGTGAAGGCTATATATCAGAACTGGAGTTGTTTAGTAAGGCTGGACACCAGAGGCTGGTTCCCTTTTTGGGACACTGCTTGGAGAATGAGAGCCAAAAGTTCTTGGTATACAAGTTTATGAGACACGGTGATTTGGCTAGCTCTTTGTTCAGGAAATCCGAAAACGAAGGTGATGGATTGAAGTCTTTGGATTGGATAACAAGGTTGAAAATTGCTCTTGGTGCAGCTGAGGGACTCTCTTATTTGCATCATGAGTGTTCACCGCCTCTAGTTCATAGGGATGTACAAGCAAGTAGCATACTTCTTGATGATAAATTTGAGGTACGTCTTGGAAGCCTAAGCGAGGCATATGCTCAAGGTGATGCTTATCAAAGCAGAATTTCTCGTTTACTTCGATTACCGCAATCGAGTGAACCATCTTCTTCAGGTGTAACAAATGCAATATGTTCGTATGATGTCTACTGCTTTGGCAAAGTGTTACTTGAGCTAGTAACGGGGAAGCTCGGGATTAGCTCGCCAGACAACGCACTTGCAAAAGAGTACATGGAAGAAGCTTTGCCATACATCAGCACAAACGAGAAAGAACTAGTAACCAAGATCTTAGACCCGTCATTAATGGTGGACGAAGACCTTCTAGAAGAAGTATGGGCAATGGCTATCATTGCAAAGTCATGCCTAAACCCAAAACCAACAAGAAGACCATTAATGAGACACATTGTGAACGCACTTGAGAACCCACTGAAAGTAGTAAGAGAAGATACCAACTCGGGTTCAGGCTCTTCCCGGTTAAGAACAAACTCGTCGAGAGGGTCTTGGAACGCTGCTATATTTGGGAGCTGGAGGCAGAGTGCGTCGGATGTAACAGCTGTTCAAGCGGGAGCAACAACAAGCGGTGGTGGTGGTGGTGGTGGTGGTAATGGTTTGAGAAACTCGGGGTCGCAAGGAAGTAGCGGAAGGAACAATAACAACAACGGGAATTCATCGTCGTCGAGAAGACGGCAATCTAGTGAGATTGTACCGGAACCTGCTGCTTATGGAGTAGTAGAGGATAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

915

Amino Acids

99.7

Weight (kDa)

8.26

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 128 - 329 6.4e-18 Leucine-rich repeat region
LRR_8 PF13855 130 - 187 4.7e-09 Leucine rich repeat
LRR_8 PF13855 177 - 235 1.3e-06 Leucine rich repeat
Pkinase PF00069 531 - 804 1.5e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 533 - 806 2.3e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1661
AatII GACGTC 1 cut(s) 1282
Acc36I ACCTGC 2 cut(s) 1480, 2722
AccB7I CCANNNNNTGG 1 cut(s) 1744
AccBSI CCGCTC 1 cut(s) 1439
AccI GTMKAC 5 cut(s) 199, 937, 1648, 1797, 2148
AccII CGCG 1 cut(s) 329
AciI CCGC 9 cut(s) 970, 1330, 1439, 1678, 1952, 2090, 2564, 2580, 2640
AclWI GGATC 3 cut(s) 236, 284, 408
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 4 cut(s) 1577, 2003, 2067, 2665
AcuI CTGAAG 5 cut(s) 617, 708, 1302, 1547, 2097
AcyI GRCGYC 1 cut(s) 1279
AdeI CACNNNGTG 1 cut(s) 2407
AfaI GTAC 6 cut(s) 57, 859, 1974, 2013, 2231, 2706
AfiI CCNNNNNNNGG 6 cut(s) 170, 659, 1502, 1744, 1757, 2032
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 7 cut(s) 472, 529, 696, 1582, 1870, 1897, 2561
AhlI ACTAGT 3 cut(s) 455, 494, 2275
AjiI CACGTC 1 cut(s) 1529
AjnI CCWGG 1 cut(s) 652
Alw21I GWGCWC 2 cut(s) 1086, 1510
Alw26I GTCTC 5 cut(s) 483, 717, 1204, 1804, 2392
AlwI GGATC 3 cut(s) 236, 284, 408
AlwNI CAGNNNCTG 1 cut(s) 1744
Ama87I CYCGRG 3 cut(s) 2192, 2455, 2620
AoxI GGCC 2 cut(s) 161, 1463
ApeKI GCWGC 8 cut(s) 823, 1009, 1235, 1352, 1555, 1913, 2510, 2717
ApoI RAATTY 4 cut(s) 1577, 2003, 2067, 2665
ArsI GACNNNNNNTTYG 2 cut(s) 79, 111
AseI ATTAAT 2 cut(s) 2303, 2393
Asp700I GAANNNNTTC 4 cut(s) 560, 995, 1070, 2319
AspLEI GCGC 2 cut(s) 23, 1425
AspS9I GGNCC 2 cut(s) 641, 1117
AsuC2I CCSGG 5 cut(s) 334, 348, 1165, 1477, 2473
AsuHPI GGTGA 6 cut(s) 185, 746, 1829, 1871, 1941, 2060
AsuII TTCGAA 3 cut(s) 1064, 1137, 1198
AsuNHI GCTAGC 1 cut(s) 1826
AvaI CYCGRG 3 cut(s) 2192, 2455, 2620
AvaII GGWCC 2 cut(s) 641, 1117
AxyI CCTNAGG 1 cut(s) 795
BalI TGGCCA 1 cut(s) 163
BanII GRGCYC 1 cut(s) 1086
BbsI GAAGAC 4 cut(s) 126, 2322, 2392, 2689
Bbv12I GWGCWC 2 cut(s) 1086, 1510
BbvCI CCTCAGC 1 cut(s) 1917
BbvI GCAGC 8 cut(s) 835, 996, 1247, 1364, 1567, 1925, 2497, 2704
BccI CCATC 3 cut(s) 690, 1856, 2113
BceAI ACGGC 2 cut(s) 1204, 2702
BcgI CGANNNNNNTGC 4 cut(s) 2021, 2055, 2118, 2152
BciT130I CCWGG 1 cut(s) 654
BclI TGATCA 1 cut(s) 7
BcnI CCSGG 5 cut(s) 334, 348, 1165, 1477, 2473
BcoDI GTCTC 5 cut(s) 483, 717, 1204, 1804, 2392
BcuI ACTAGT 3 cut(s) 455, 494, 2275
BfuAI ACCTGC 2 cut(s) 1480, 2722
BglII AGATCT 1 cut(s) 2286
Bme1390I CCNGG 6 cut(s) 334, 348, 654, 1165, 1477, 2473
Bme18I GGWCC 2 cut(s) 641, 1117
BmeT110I CYCGRG 3 cut(s) 2192, 2455, 2620
BmgBI CACGTC 1 cut(s) 1529
BmgT120I GGNCC 2 cut(s) 641, 1117
BmiI GGNNCC 2 cut(s) 1749, 2712
BmrFI CCNGG 6 cut(s) 334, 348, 654, 1165, 1477, 2473
BmsI GCATC 4 cut(s) 777, 1576, 1945, 2041
BmtI GCTAGC 1 cut(s) 1830
BpiI GAAGAC 4 cut(s) 126, 2322, 2392, 2689
BplI GAGNNNNNCTC 2 cut(s) 1910, 1942
BpmI CTGGAG 2 cut(s) 1733, 2548
Bpu10I CCTNAGC 2 cut(s) 1917, 2027
Bpu14I TTCGAA 3 cut(s) 1064, 1137, 1198
BpuEI CTTGAG 3 cut(s) 2028, 2192, 2438
BpuMI CCSGG 5 cut(s) 334, 348, 1165, 1477, 2473
Bsa29I ATCGAT 1 cut(s) 1671
BsaBI GATNNNNATC 3 cut(s) 233, 1590, 2055
BsaHI GRCGYC 1 cut(s) 1279
BsaJI CCNNGG 3 cut(s) 347, 1164, 1476
BsaWI WCCGGW 1 cut(s) 2707
BsaXI ACNNNNNCTCC 2 cut(s) 2521, 2551
Bsc4I CCNNNNNNNGG 6 cut(s) 170, 659, 1502, 1744, 1757, 2032
Bse1I ACTGG 4 cut(s) 571, 643, 866, 1716
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 3 cut(s) 981, 2346, 2349
Bse8I GATNNNNATC 3 cut(s) 233, 1590, 2055
BseBI CCWGG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 1671
BseDI CCNNGG 3 cut(s) 347, 1164, 1476
BseGI GGATG 2 cut(s) 1975, 2551
BseJI GATNNNNATC 3 cut(s) 233, 1590, 2055
BseLI CCNNNNNNNGG 6 cut(s) 170, 659, 1502, 1744, 1757, 2032
BseMI GCAATG 3 cut(s) 981, 2346, 2349
BseMII CTCAG 7 cut(s) 142, 351, 494, 778, 809, 876, 1908
BseNI ACTGG 4 cut(s) 571, 643, 866, 1716
BseRI GAGGAG 1 cut(s) 1222
BseXI GCAGC 8 cut(s) 835, 996, 1247, 1364, 1567, 1925, 2497, 2704
BsgI GTGCAG 2 cut(s) 1254, 1932
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 2 cut(s) 163, 1465
BshVI ATCGAT 1 cut(s) 1671
BsiHKAI GWGCWC 2 cut(s) 1086, 1510
BsiHKCI CYCGRG 3 cut(s) 2192, 2455, 2620
BsiSI CCGG 6 cut(s) 333, 347, 1164, 1476, 2473, 2708
BslFI GGGAC 3 cut(s) 364, 1773, 1935
BslI CCNNNNNNNGG 6 cut(s) 170, 659, 1502, 1744, 1757, 2032
BsmAI GTCTC 5 cut(s) 483, 717, 1204, 1804, 2392
BsmBI CGTCTC 1 cut(s) 717
BsmFI GGGAC 3 cut(s) 364, 1773, 1935
BsmI GAATGC 1 cut(s) 1238
BsnI GGCC 2 cut(s) 163, 1465
BsoBI CYCGRG 3 cut(s) 2192, 2455, 2620
Bsp119I TTCGAA 3 cut(s) 1064, 1137, 1198
Bsp1286I GDGCHC 2 cut(s) 1086, 1510
Bsp1407I TGTACA 1 cut(s) 1972
Bsp143I GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
BspACI CCGC 9 cut(s) 970, 1330, 1439, 1678, 1952, 2090, 2564, 2580, 2640
BspANI GGCC 2 cut(s) 163, 1465
BspCNI CTCAG 7 cut(s) 141, 352, 493, 777, 808, 877, 1909
BspDI ATCGAT 1 cut(s) 1671
BspFNI CGCG 1 cut(s) 329
BspHI TCATGA 1 cut(s) 1939
BspLI GGNNCC 2 cut(s) 1749, 2712
BspMI ACCTGC 2 cut(s) 1480, 2722
BspOI GCTAGC 1 cut(s) 1830
BspPI GGATC 3 cut(s) 236, 284, 408
BspQI GCTCTTC 1 cut(s) 2473
BspT104I TTCGAA 3 cut(s) 1064, 1137, 1198
BsrBI CCGCTC 1 cut(s) 1439
BsrDI GCAATG 3 cut(s) 981, 2346, 2349
BsrGI TGTACA 1 cut(s) 1972
BsrI ACTGG 4 cut(s) 571, 643, 866, 1716
BssECI CCNNGG 3 cut(s) 347, 1164, 1476
BssMI GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
BssNAI GTATAC 3 cut(s) 200, 1649, 1798
BssNI GRCGYC 1 cut(s) 1279
Bst1107I GTATAC 3 cut(s) 200, 1649, 1798
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 8 cut(s) 60, 71, 425, 488, 522, 1104, 1339, 1817
Bst6I CTCTTC 3 cut(s) 300, 1066, 2473
BstACI GRCGYC 1 cut(s) 1279
BstAPI GCANNNNNTGC 1 cut(s) 1232
BstAUI TGTACA 1 cut(s) 1972
BstBI TTCGAA 3 cut(s) 1064, 1137, 1198
BstC8I GCNNGC 3 cut(s) 1421, 1828, 2204
BstEII GGTNACC 1 cut(s) 173
BstENI CCTNNNNNAGG 2 cut(s) 657, 2030
BstF5I GGATG 2 cut(s) 1975, 2551
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 2 cut(s) 23, 1425
BstKTI GATC 9 cut(s) 10, 231, 292, 403, 547, 739, 763, 1671, 2289
BstMAI GTCTC 5 cut(s) 483, 717, 1204, 1804, 2392
BstMBI GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
BstMWI GCNNNNNNNGC 7 cut(s) 967, 1232, 1431, 1481, 1910, 2351, 2561
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 1 cut(s) 1423
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 6 cut(s) 332, 346, 652, 1163, 1475, 2471
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 8 cut(s) 835, 996, 1247, 1364, 1567, 1925, 2497, 2704
BstV2I GAAGAC 4 cut(s) 126, 2322, 2392, 2689
BstX2I RGATCY 2 cut(s) 400, 2286
BstYI RGATCY 2 cut(s) 400, 2286
BstZ17I GTATAC 3 cut(s) 200, 1649, 1798
Bsu15I ATCGAT 1 cut(s) 1671
Bsu36I CCTNAGG 1 cut(s) 795
BsuRI GGCC 2 cut(s) 163, 1465
BsuTUI ATCGAT 1 cut(s) 1671
BtrI CACGTC 1 cut(s) 1529
BtsCI GGATG 2 cut(s) 1975, 2551
BtsI GCAGTG 2 cut(s) 1362, 1762
BtsIMutI CAGTG 5 cut(s) 109, 564, 1362, 1762, 2426
BveI ACCTGC 2 cut(s) 1480, 2722
Cac8I GCNNGC 3 cut(s) 1421, 1828, 2204
CaiI CAGNNNCTG 1 cut(s) 1744
CciI TCATGA 1 cut(s) 1939
CfoI GCGC 2 cut(s) 23, 1425
Cfr13I GGNCC 2 cut(s) 641, 1117
ClaI ATCGAT 1 cut(s) 1671
CseI GACGC 3 cut(s) 318, 1440, 2529
Csp6I GTAC 6 cut(s) 56, 858, 1973, 2012, 2230, 2705
CviAII CATG 4 cut(s) 1420, 1940, 2233, 2361
CviQI GTAC 6 cut(s) 56, 858, 1973, 2012, 2230, 2705
DpnI GATC 9 cut(s) 9, 230, 291, 402, 546, 738, 762, 1670, 2288
DpnII GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
DraIII CACNNNGTG 1 cut(s) 2407
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 3 cut(s) 300, 1066, 2473
EarI CTCTTC 3 cut(s) 300, 1066, 2473
Ecl136II GAGCTC 1 cut(s) 1084
Eco24I GRGCYC 1 cut(s) 1086
Eco32I GATATC 1 cut(s) 1057
Eco47I GGWCC 2 cut(s) 641, 1117
Eco53kI GAGCTC 1 cut(s) 1084
Eco57I CTGAAG 5 cut(s) 617, 708, 1302, 1547, 2097
Eco81I CCTNAGG 1 cut(s) 795
Eco88I CYCGRG 3 cut(s) 2192, 2455, 2620
Eco91I GGTNACC 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1084
EcoNI CCTNNNNNAGG 2 cut(s) 657, 2030
EcoO65I GGTNACC 1 cut(s) 173
EcoRI GAATTC 2 cut(s) 1577, 2665
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 1057
EcoT22I ATGCAT 2 cut(s) 1421, 1543
EcoT38I GRGCYC 1 cut(s) 1086
Esp3I CGTCTC 1 cut(s) 717
FaeI CATG 4 cut(s) 1423, 1943, 2236, 2364
FalI AAGNNNNNCTT 6 cut(s) 980, 1012, 1131, 1163, 2154, 2186
FaqI GGGAC 3 cut(s) 364, 1773, 1935
FatI CATG 4 cut(s) 1419, 1939, 2232, 2360
FauI CCCGC 2 cut(s) 1671, 2557
FauNDI CATATG 1 cut(s) 2038
FbaI TGATCA 1 cut(s) 7
FblI GTMKAC 5 cut(s) 199, 937, 1648, 1797, 2148
FokI GGATG 2 cut(s) 1982, 2558
FriOI GRGCYC 1 cut(s) 1086
GlaI GCGC 2 cut(s) 22, 1424
GsuI CTGGAG 2 cut(s) 1733, 2548
HaeIII GGCC 2 cut(s) 163, 1465
HapII CCGG 6 cut(s) 333, 347, 1164, 1476, 2473, 2708
HgaI GACGC 3 cut(s) 318, 1440, 2529
HhaI GCGC 2 cut(s) 23, 1425
Hin1I GRCGYC 1 cut(s) 1279
Hin1II CATG 4 cut(s) 1423, 1943, 2236, 2364
Hin6I GCGC 2 cut(s) 21, 1423
HinP1I GCGC 2 cut(s) 21, 1423
HincII GTYRAC 3 cut(s) 421, 609, 754
HindII GTYRAC 3 cut(s) 421, 609, 754
HindIII AAGCTT 4 cut(s) 674, 1145, 1482, 2241
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 6 cut(s) 333, 347, 1164, 1476, 2473, 2708
HphI GGTGA 6 cut(s) 185, 746, 1829, 1871, 1941, 2060
Hpy99I CGWCG 4 cut(s) 2494, 2545, 2677, 2680
HpyAV CCTTC 8 cut(s) 732, 1133, 1205, 1676, 1688, 1850, 2330, 2637
HpyCH4III ACNGT 8 cut(s) 60, 71, 425, 488, 522, 1104, 1339, 1817
HpyCH4IV ACGT 4 cut(s) 1279, 1528, 1603, 2014
HpyF10VI GCNNNNNNNGC 7 cut(s) 967, 1232, 1431, 1481, 1910, 2351, 2561
HpySE526I ACGT 4 cut(s) 1279, 1528, 1603, 2014
Hsp92I GRCGYC 1 cut(s) 1279
Hsp92II CATG 4 cut(s) 1423, 1943, 2236, 2364
HspAI GCGC 2 cut(s) 21, 1423
Ksp22I TGATCA 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
LguI GCTCTTC 1 cut(s) 2473
LmnI GCTCC 5 cut(s) 662, 799, 1505, 2523, 2567
Lsp1109I GCAGC 8 cut(s) 835, 996, 1247, 1364, 1567, 1925, 2497, 2704
LweI GCATC 4 cut(s) 777, 1576, 1945, 2041
MaeII ACGT 4 cut(s) 1279, 1528, 1603, 2014
MalI GATC 9 cut(s) 9, 230, 291, 402, 546, 738, 762, 1670, 2288
MbiI CCGCTC 1 cut(s) 1439
MboI GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
MfeI CAATTG 1 cut(s) 1189
MflI RGATCY 2 cut(s) 400, 2286
MhlI GDGCHC 2 cut(s) 1086, 1510
MlsI TGGCCA 1 cut(s) 163
MluI ACGCGT 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 163
MlyI GAGTC 3 cut(s) 103, 604, 1917
MmeI TCCRAC 1 cut(s) 2523
Mox20I TGGCCA 1 cut(s) 163
Mph1103I ATGCAT 2 cut(s) 1421, 1543
MroXI GAANNNNTTC 4 cut(s) 560, 995, 1070, 2319
MscI TGGCCA 1 cut(s) 163
MslI CAYNNNNRTG 1 cut(s) 1968
Msp20I TGGCCA 1 cut(s) 163
MspA1I CMGCKG 2 cut(s) 1916, 2555
MspI CCGG 6 cut(s) 333, 347, 1164, 1476, 2473, 2708
MspR9I CCNGG 6 cut(s) 334, 348, 654, 1165, 1477, 2473
MunI CAATTG 1 cut(s) 1189
Mva1269I GAATGC 1 cut(s) 1238
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 7 cut(s) 967, 1232, 1431, 1481, 1910, 2351, 2561
NciI CCSGG 5 cut(s) 334, 348, 1165, 1477, 2473
NdeI CATATG 1 cut(s) 2038
NdeII GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
NheI GCTAGC 1 cut(s) 1826
NlaIII CATG 4 cut(s) 1423, 1943, 2236, 2364
NlaIV GGNNCC 2 cut(s) 1749, 2712
NmuCI GTSAC 3 cut(s) 173, 1159, 1524
NsiI ATGCAT 2 cut(s) 1421, 1543
NspI RCATGY 1 cut(s) 1423
NspV TTCGAA 3 cut(s) 1064, 1137, 1198
PaeI GCATGC 1 cut(s) 1423
PagI TCATGA 1 cut(s) 1939
PciSI GCTCTTC 1 cut(s) 2473
PcsI WCGNNNNNNNCGW 1 cut(s) 2080
PctI GAATGC 1 cut(s) 1238
PdmI GAANNNNTTC 4 cut(s) 560, 995, 1070, 2319
PfeI GAWTC 9 cut(s) 99, 472, 529, 601, 718, 973, 1028, 1093, 1618
PflMI CCANNNNNTGG 1 cut(s) 1744
PleI GAGTC 3 cut(s) 102, 604, 1917
PpsI GAGTC 3 cut(s) 102, 604, 1917
PshBI ATTAAT 2 cut(s) 2303, 2393
PsiI TTATAA 1 cut(s) 1661
Psp124BI GAGCTC 1 cut(s) 1086
Psp6I CCWGG 1 cut(s) 652
PspEI GGTNACC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 2 cut(s) 1749, 2712
PspPI GGNCC 2 cut(s) 641, 1117
PsrI GAACNNNNNNTAC 2 cut(s) 2541, 2573
PstNI CAGNNNCTG 1 cut(s) 1744
PsuI RGATCY 2 cut(s) 400, 2286
PvuII CAGCTG 2 cut(s) 1916, 2555
RsaI GTAC 6 cut(s) 57, 859, 1974, 2013, 2231, 2706
RsaNI GTAC 6 cut(s) 56, 858, 1973, 2012, 2230, 2705
RseI CAYNNNNRTG 1 cut(s) 1968
SacI GAGCTC 1 cut(s) 1086
SapI GCTCTTC 1 cut(s) 2473
Sau3AI GATC 9 cut(s) 7, 228, 289, 400, 544, 736, 760, 1668, 2286
Sau96I GGNCC 2 cut(s) 641, 1117
SchI GAGTC 3 cut(s) 103, 604, 1917
ScrFI CCNGG 6 cut(s) 334, 348, 654, 1165, 1477, 2473
SduI GDGCHC 2 cut(s) 1086, 1510
SfaNI GCATC 4 cut(s) 777, 1576, 1945, 2041
SfuI TTCGAA 3 cut(s) 1064, 1137, 1198
SinI GGWCC 2 cut(s) 641, 1117
SmiMI CAYNNNNRTG 1 cut(s) 1968
SmlI CTYRAG 3 cut(s) 2043, 2171, 2417
SmoI CTYRAG 3 cut(s) 2043, 2171, 2417
SpeI ACTAGT 3 cut(s) 455, 494, 2275
SphI GCATGC 1 cut(s) 1423
SsiI CCGC 9 cut(s) 970, 1330, 1439, 1678, 1952, 2090, 2564, 2580, 2640
SstI GAGCTC 1 cut(s) 1086
StyD4I CCNGG 6 cut(s) 332, 346, 652, 1163, 1475, 2471
TaaI ACNGT 8 cut(s) 60, 71, 425, 488, 522, 1104, 1339, 1817
TaiI ACGT 4 cut(s) 1282, 1531, 1606, 2017
TatI WGTACW 2 cut(s) 1972, 2229
TauI GCSGC 2 cut(s) 1332, 1442
TfiI GAWTC 9 cut(s) 99, 472, 529, 601, 718, 973, 1028, 1093, 1618
TscAI CASTG 5 cut(s) 109, 571, 1362, 1769, 2433
TseFI GTSAC 3 cut(s) 173, 1159, 1524
TseI GCWGC 8 cut(s) 823, 1009, 1235, 1352, 1555, 1913, 2510, 2717
Tsp45I GTSAC 3 cut(s) 173, 1159, 1524
TspDTI ATGAA 3 cut(s) 765, 1952, 2658
TspRI CASTG 5 cut(s) 109, 571, 1362, 1769, 2433
Van91I CCANNNNNTGG 1 cut(s) 1744
VpaK11BI GGWCC 2 cut(s) 641, 1117
VspI ATTAAT 2 cut(s) 2303, 2393
XagI CCTNNNNNAGG 2 cut(s) 657, 2030
XapI RAATTY 4 cut(s) 1577, 2003, 2067, 2665
XbaI TCTAGA 2 cut(s) 1250, 2323
XceI RCATGY 1 cut(s) 1423
XmiI GTMKAC 5 cut(s) 199, 937, 1648, 1797, 2148
XmnI GAANNNNTTC 4 cut(s) 560, 995, 1070, 2319
ZraI GACGTC 1 cut(s) 1280
Zsp2I ATGCAT 2 cut(s) 1421, 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.