RLG00000014252

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002531
Physical Location & Seq
Forward (+)
50078540 .. 50083643
5104 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014252

Sequence Viewer

Length: 2637 bp
ATGGTGGATCAAAGGAGCATAGTATGGTGTTTCTTGTTGTTGTGGGCGGTGTTGGCGTTTCAGCCCACATTTCAGCAACCGAGTCAGCAGCCACTGAGTTCACACGCCGACCGAGTTGCTCTGCTTCAGCTCAGATCGTCTTTGGGTTTGAGGGGCAGGGACTGGCCCATTAAGTCAGACCCTTGCTTCGTTTGGAAGGGTGTTGAATGCAGTAATGGTAGGGTTGTTGGGATTAACGTTTCTGGGTTTAGAAGAACTAGACTTGGGAGTCAAAACCCACAATTTTCAGTCGATGCTTTGGCCAATTTGACCCTTTTGAGTTCTTTCAATGCCTCCAATTTCTTGCTTCCCGGTTCCATTCCTGACTGGTTTGGCCAAAGGCTCGGGTCTCTGCGTGTTCTTGATCTCACTTCTTGTTCTGTGAATGGTCCTATTCCATTGAGTCTTGGAAATTCTACCAATCTGACTGGTCTGTCCTTGTCTCGTAATAACGTTACCGGGACAATTCCTGCTAGTTTGAGCAACTTGGTAGGCCTTTTGGTCCTTGACCTCTCTCAAAATCGGCTGACTGGATCCATTCCCACATCTTTTGGGAATCTTGGGAACCTTTCTGTTCTTGACATTTCTGGGAATTACTTAACCGGGTCAATTCCTCCGGGCATTGGGAACCTCTCGAAGCTTCAATCTCTGAATCTTTCCAGCAACATGTTGTCTTCTTCCATTCCTCCTGAACTTGGGGGCCTTGATGCCTTGGTTGACCTTGATCTCAGCTCCAATCTATTGGTGGGTCTGCTGCCTTCGGGTTTGAGGAACTTGCAGAGAATTGTAGTTGCGGACAATTTTCTCAGTGGTTCTGTGGATGTGCTCTGGTCAATGCCTCAATTGAGTTTTCTTGATGTGGCTGGAAACAATTTCACTGGTTTGCTGCCCAATTCTAGCTCGAATGCTAATGCCACTGCAGCAGTATTTAATATTTCTCATAATTTGTTTTATGGGCCTATTACACCATTAGTTGGAAAATTTGGTGCTATTGATCTTTCCGGAAACTATTTTGAAGGTGAGGTACCAGGTTATGTTGGCACCAATGCATCTCTTGACAGCAATTGTCTCCAAAATTTGAAGAATCAGAGGACTTTGGCAGAATGTTCATCGTTTTATGCTAATAGGGGTTTGAGTTTTGATAATTTTGGGCAGCCAAACTCTACACAACCTCCTGCAGCCGCACCACCTGGGAAGAAAAGTAATAAAAATGTGATCATATTGGCATCAGTTTTAGGAGGAGTTGCTTTGATTGTGGTTTTGGTCTTGTTTTTAGTAATAGTCCTATGTTCACGCAAGCGGGGGGGAGCTACAACTCAAAGGGGGGTTGGTGTGGGGTCAGTTCCTGCAGGCAGCAGTCCCCCACCAGCTGGAACATCGGTTAACTTTTCTAGCGTAGGAGATGCATTCACATATCAGCAGCTGCTTCAAGCTACGGGTGACTTCAACAATACGAACTTAATGAAAAATGGCCACACAGGGGATCTCTTCCGGGGTGTCTTGGAAAATGGGATCCCTGTGGTTATTAAAAGGATTGATTTACGTTCGACTAAAAAGGAAGCGTACCTGCTGGAGTTGGATTTCTTTAGTAAGGTGTCAAGTACAAGATTTGTTCCCTTTCTGGGTCACTGTTTAGAGAATGAGAACGAGAAGTTCATGGTGTACAAATACATGCCAAATGGGGACCTATCAAGTTCTCTGTACAAGAAAACCAACACCCTGGATGATACTTTACAGTCATTGGATTGGATCACTAGATTAAAGATCGCATTAGGTGCTGCGGAGGGTCTCACCTATCTGCATCATGAGTGCGATCCACCCCTCGTGCACAGAGATGTGCAAGCAAGCAGTGTACTTCTTGATGATAAATTTGAAGTGCGGCTAGGAAGCCTGAGTGATGTCTGTGCTCAAGAAGGAGATACTCATCAAAGCAGGATTACTAGGTTGCTGCGGTTGCCACAGTCATCCGAACAAGGTGCTTCTGGTTCATCGACAGCATCATGTGCCTATGATGTTTATTGTTTCGGGAAGGTATTACTTGAACTGGTAACAGGTAAATTGGGCATCAGTGCTTCCAGTGAATCCCAATTGAAGGAATTGTTAGATGACATACTTCCCTACATCAGTATATATGACAATGAACTTGTGACAAAAATTGTGGATCCATCTTTGATTGTGGATGAGGACTTATTAGAGGAAGTGTGGGCTATGGCCGTCGTGGCCAGGTCCTGCCTAAATCCCAAGCCCTCAAGGCGCCCGCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTGTTAAGGTAGTAAGGGAAGATAGTACTGGCTCTGCAAGGCTCAGAAAAGCTTCTTCTCGAGGGTCTTGGAATGCTGCTGTCTTTGGTAGCTGGCGCAGCTCATCTGAGGTAGTGGTTATTCCAGGAGCCTCAGGTACCAAAGCTGAAGGAGTGAGTGGTTTGAAGCATTCAACAACCACCGGTTCGCAGGGAAGTGGCCAAAATGGTGGGGGTGAACATTCATCCTCACGTAGACGGCATTCTAGGGATATTTTCCCTGAACCATCTGGTGTACAAGATGTAGAGAGATTTGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

879

Amino Acids

94.25

Weight (kDa)

6.84

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 130 - 275 1.7e-14 Leucine-rich repeat region
LRR_8 PF13855 154 - 213 1.7e-09 Leucine rich repeat
LRR_LRWD1 PF23211 222 - 307 8.1e-07 Leucine-rich repeat and WD repeat-containing protein 1, LRR
LRR_8 PF13855 223 - 261 6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 501 - 774 3.3e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 503 - 772 1e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 267, 472
Acc36I ACCTGC 1 cut(s) 1614
Acc65I GGTACC 2 cut(s) 1063, 2468
AccB1I GGYRCC 4 cut(s) 1063, 1079, 2292, 2468
AccB7I CCANNNNNTGG 2 cut(s) 1013, 1409
AccI GTMKAC 1 cut(s) 2566
AccIII TCCGGA 1 cut(s) 1040
AciI CCGC 8 cut(s) 47, 833, 1221, 1339, 1820, 1918, 1990, 2297
AclI AACGTT 2 cut(s) 237, 492
AcoI YGGCCR 6 cut(s) 300, 373, 1510, 2250, 2259, 2530
AcsI RAATTY 4 cut(s) 451, 1019, 1114, 1907
AcuI CTGAAG 2 cut(s) 110, 2499
AcyI GRCGYC 1 cut(s) 2293
AfaI GTAC 9 cut(s) 1065, 1604, 1642, 1703, 1742, 1893, 2359, 2470, 2607
AfiI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1409, 1519, 1661, 2131, 2338
AflIII ACRYGT 1 cut(s) 705
AgeI ACCGGT 1 cut(s) 2513
AjnI CCWGG 6 cut(s) 1066, 1228, 1758, 2261, 2455, 2628
Alw21I GWGCWC 3 cut(s) 867, 1869, 1948
Alw26I GTCTC 4 cut(s) 393, 486, 1112, 1832
Alw44I GTGCAC 1 cut(s) 1865
AlwNI CAGNNNCTG 5 cut(s) 94, 162, 1385, 1462, 2268
Ama87I CYCGRG 2 cut(s) 383, 2391
Aor13HI TCCGGA 1 cut(s) 1040
ApaLI GTGCAC 1 cut(s) 1865
ApoI RAATTY 4 cut(s) 451, 1019, 1114, 1907
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AsiGI ACCGGT 1 cut(s) 2513
Asp700I GAANNNNTTC 1 cut(s) 2383
Asp718I GGTACC 2 cut(s) 1063, 2468
AspLEI GCGC 2 cut(s) 2295, 2430
AspS9I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1723, 2265
AsuC2I CCSGG 5 cut(s) 351, 499, 643, 657, 1532
AsuHPI GGTGA 4 cut(s) 1070, 1490, 1822, 2558
AvaI CYCGRG 2 cut(s) 383, 2391
AvaII GGWCC 4 cut(s) 428, 541, 1723, 2265
AxyI CCTNAGG 1 cut(s) 2464
BaeGI GKGCMC 1 cut(s) 1869
BalI TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
BamHI GGATCC 3 cut(s) 572, 1551, 2200
BanI GGYRCC 4 cut(s) 1063, 1079, 2292, 2468
BarI GAAGNNNNNNTAC 2 cut(s) 1047, 1079
BauI CACGAG 1 cut(s) 1862
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 3 cut(s) 867, 1869, 1948
BccI CCATC 2 cut(s) 2212, 2605
BceAI ACGGC 2 cut(s) 2237, 2585
BcgI CGANNNNNNTGC 4 cut(s) 98, 132, 1997, 2031
BciT130I CCWGG 6 cut(s) 1068, 1230, 1760, 2263, 2457, 2630
BclI TGATCA 1 cut(s) 1254
BcnI CCSGG 5 cut(s) 351, 499, 643, 657, 1532
BcoDI GTCTC 4 cut(s) 393, 486, 1112, 1832
BfaI CTAG 9 cut(s) 258, 513, 936, 1431, 1794, 1922, 1980, 2577, 2635
BfmI CTRYAG 3 cut(s) 957, 1215, 1386
BfoI RGCGCY 1 cut(s) 2296
BfuAI ACCTGC 1 cut(s) 1614
BglI GCCNNNNNGGC 2 cut(s) 2258, 2290
BmcAI AGTACT 1 cut(s) 2359
Bme18I GGWCC 4 cut(s) 428, 541, 1723, 2265
BmeT110I CYCGRG 2 cut(s) 383, 2391
BmgT120I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1723, 2265
BmsI GCATC 8 cut(s) 283, 736, 1097, 1274, 1432, 1849, 2045, 2112
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 1 cut(s) 1631
BpuEI CTTGAG 3 cut(s) 1932, 2272, 2300
BpuMI CCSGG 5 cut(s) 351, 499, 643, 657, 1532
BsaAI YACGTR 1 cut(s) 2564
BsaBI GATNNNNATC 1 cut(s) 1962
BsaHI GRCGYC 1 cut(s) 2293
BsaI GGTCTC 2 cut(s) 393, 1832
BsaJI CCNNGG 4 cut(s) 750, 1229, 1531, 1758
BsaWI WCCGGW 2 cut(s) 1040, 2513
BsaXI ACNNNNNCTCC 2 cut(s) 1195, 1225
Bsc4I CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1409, 1519, 1661, 2131, 2338
Bse118I RCCGGY 1 cut(s) 2513
Bse1I ACTGG 8 cut(s) 167, 371, 472, 574, 922, 2088, 2115, 2365
Bse21I CCTNAGG 1 cut(s) 2464
Bse8I GATNNNNATC 1 cut(s) 1962
BseAI TCCGGA 1 cut(s) 1040
BseBI CCWGG 6 cut(s) 1068, 1230, 1760, 2263, 2457, 2630
BseDI CCNNGG 4 cut(s) 750, 1229, 1531, 1758
BseGI GGATG 5 cut(s) 865, 1768, 2003, 2224, 2555
BseJI GATNNNNATC 1 cut(s) 1962
BseLI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1409, 1519, 1661, 2131, 2338
BseMII CTCAG 8 cut(s) 86, 145, 781, 859, 1922, 2389, 2430, 2478
BseNI ACTGG 8 cut(s) 167, 371, 472, 574, 922, 2088, 2115, 2365
BseRI GAGGAG 1 cut(s) 1293
BseSI GKGCMC 1 cut(s) 1869
Bsh1285I CGRYCG 1 cut(s) 112
BshNI GGYRCC 4 cut(s) 1063, 1079, 2292, 2468
BshTI ACCGGT 1 cut(s) 2513
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 3 cut(s) 867, 1869, 1948
BsiHKCI CYCGRG 2 cut(s) 383, 2391
BsiSI CCGG 7 cut(s) 351, 498, 642, 656, 1041, 1531, 2514
BslFI GGGAC 4 cut(s) 173, 514, 1383, 1736
BslI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1409, 1519, 1661, 2131, 2338
BsmAI GTCTC 4 cut(s) 393, 486, 1112, 1832
BsmFI GGGAC 4 cut(s) 173, 514, 1383, 1736
BsmI GAATGC 6 cut(s) 212, 949, 1445, 2410, 2500, 2572
Bso31I GGTCTC 2 cut(s) 393, 1832
BsoBI CYCGRG 2 cut(s) 383, 2391
Bsp1286I GDGCHC 3 cut(s) 867, 1869, 1948
Bsp13I TCCGGA 1 cut(s) 1040
Bsp1407I TGTACA 3 cut(s) 1701, 1740, 2605
BspACI CCGC 8 cut(s) 47, 833, 1221, 1339, 1820, 1918, 1990, 2297
BspCNI CTCAG 8 cut(s) 87, 144, 780, 858, 1923, 2388, 2431, 2477
BspEI TCCGGA 1 cut(s) 1040
BspHI TCATGA 1 cut(s) 1843
BspMAI CTGCAG 3 cut(s) 961, 1219, 1390
BspMI ACCTGC 1 cut(s) 1614
BspT107I GGYRCC 4 cut(s) 1063, 1079, 2292, 2468
BspTNI GGTCTC 2 cut(s) 393, 1832
BsrFI RCCGGY 1 cut(s) 2513
BsrGI TGTACA 3 cut(s) 1701, 1740, 2605
BsrI ACTGG 8 cut(s) 167, 371, 472, 574, 922, 2088, 2115, 2365
BssAI RCCGGY 1 cut(s) 2513
BssECI CCNNGG 4 cut(s) 750, 1229, 1531, 1758
BssNI GRCGYC 1 cut(s) 2293
BssSI CACGAG 1 cut(s) 1862
BssT1I CCWWGG 1 cut(s) 750
Bst2BI CACGAG 1 cut(s) 1862
Bst2UI CCWGG 6 cut(s) 1068, 1230, 1760, 2263, 2457, 2630
Bst4CI ACNGT 3 cut(s) 1670, 1776, 2001
Bst6I CTCTTC 1 cut(s) 1532
BstACI GRCGYC 1 cut(s) 2293
BstAPI GCANNNNNTGC 2 cut(s) 1814, 2042
BstAUI TGTACA 3 cut(s) 1701, 1740, 2605
BstBAI YACGTR 1 cut(s) 2564
BstC8I GCNNGC 6 cut(s) 1337, 1390, 1881, 1885, 2297, 2426
BstDEI CTNAG 8 cut(s) 95, 131, 767, 845, 1931, 2375, 2439, 2464
BstF5I GGATG 5 cut(s) 865, 1768, 2003, 2224, 2555
BstH2I RGCGCY 1 cut(s) 2296
BstHHI GCGC 2 cut(s) 2295, 2430
BstMAI GTCTC 4 cut(s) 393, 486, 1112, 1832
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 8 cut(s) 53, 959, 1814, 1993, 2042, 2258, 2290, 2430
BstNI CCWGG 6 cut(s) 1068, 1230, 1760, 2263, 2457, 2630
BstNSI RCATGY 2 cut(s) 709, 1714
BstSFI CTRYAG 3 cut(s) 957, 1215, 1386
BstSLI GKGCMC 1 cut(s) 1869
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 4 cut(s) 572, 1522, 1551, 2200
BstXI CCANNNNNNTGG 3 cut(s) 781, 1759, 2540
BstYI RGATCY 4 cut(s) 572, 1522, 1551, 2200
Bsu36I CCTNAGG 1 cut(s) 2464
BtsCI GGATG 5 cut(s) 865, 1768, 2003, 2224, 2555
BtsI GCAGTG 2 cut(s) 954, 1894
BtsIMutI CAGTG 8 cut(s) 92, 853, 915, 954, 1666, 1894, 2113, 2122
BveI ACCTGC 1 cut(s) 1614
Cac8I GCNNGC 6 cut(s) 1337, 1390, 1881, 1885, 2297, 2426
CaiI CAGNNNCTG 5 cut(s) 94, 162, 1385, 1462, 2268
CciI TCATGA 1 cut(s) 1843
CfoI GCGC 2 cut(s) 2295, 2430
Cfr10I RCCGGY 1 cut(s) 2513
Cfr13I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1723, 2265
CsiI ACCWGGT 1 cut(s) 1066
Csp6I GTAC 9 cut(s) 1064, 1603, 1641, 1702, 1741, 1892, 2358, 2469, 2606
CspAI ACCGGT 1 cut(s) 2513
CspCI CAANNNNNGTGG 4 cut(s) 571, 606, 2178, 2213
CviAII CATG 5 cut(s) 706, 1696, 1711, 1844, 2040
CviQI GTAC 9 cut(s) 1064, 1603, 1641, 1702, 1741, 1892, 2358, 2469, 2606
DdeI CTNAG 8 cut(s) 95, 131, 767, 845, 1931, 2375, 2439, 2464
DinI GGCGCC 1 cut(s) 2294
DrdI GACNNNNNNGTC 2 cut(s) 267, 472
DseDI GACNNNNNNGTC 2 cut(s) 267, 472
EaeI YGGCCR 6 cut(s) 300, 373, 1510, 2250, 2259, 2530
Eam1104I CTCTTC 1 cut(s) 1532
EarI CTCTTC 1 cut(s) 1532
Eco130I CCWWGG 1 cut(s) 750
Eco147I AGGCCT 1 cut(s) 534
Eco31I GGTCTC 2 cut(s) 393, 1832
Eco47I GGWCC 4 cut(s) 428, 541, 1723, 2265
Eco57I CTGAAG 2 cut(s) 110, 2499
Eco81I CCTNAGG 1 cut(s) 2464
Eco88I CYCGRG 2 cut(s) 383, 2391
EcoO109I RGGNCCY 3 cut(s) 739, 1723, 2265
EcoRII CCWGG 6 cut(s) 1066, 1228, 1758, 2261, 2455, 2628
EcoT14I CCWWGG 1 cut(s) 750
EcoT22I ATGCAT 2 cut(s) 1090, 1447
EgeI GGCGCC 1 cut(s) 2294
EheI GGCGCC 1 cut(s) 2294
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 5 cut(s) 709, 1699, 1714, 1847, 2043
FaqI GGGAC 4 cut(s) 173, 514, 1383, 1736
FatI CATG 5 cut(s) 705, 1695, 1710, 1843, 2039
FauI CCCGC 2 cut(s) 1332, 2304
FbaI TGATCA 1 cut(s) 1254
FblI GTMKAC 1 cut(s) 2566
FokI GGATG 5 cut(s) 872, 1775, 1990, 2231, 2542
FspBI CTAG 9 cut(s) 258, 513, 936, 1431, 1794, 1922, 1980, 2577, 2635
GlaI GCGC 2 cut(s) 2294, 2429
GsuI CTGGAG 1 cut(s) 1631
HaeII RGCGCY 1 cut(s) 2296
HapII CCGG 7 cut(s) 351, 498, 642, 656, 1041, 1531, 2514
HhaI GCGC 2 cut(s) 2295, 2430
Hin1I GRCGYC 1 cut(s) 2293
Hin1II CATG 5 cut(s) 709, 1699, 1714, 1847, 2043
Hin6I GCGC 2 cut(s) 2293, 2428
HinP1I GCGC 2 cut(s) 2293, 2428
HincII GTYRAC 2 cut(s) 757, 1423
HindII GTYRAC 2 cut(s) 757, 1423
HindIII AAGCTT 2 cut(s) 677, 2382
HinfI GANTC 7 cut(s) 82, 268, 442, 595, 691, 1123, 2120
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 7 cut(s) 351, 498, 642, 656, 1041, 1531, 2514
HphI GGTGA 4 cut(s) 1070, 1490, 1822, 2558
Hpy188I TCNGA 8 cut(s) 134, 178, 465, 690, 1128, 2008, 2378, 2440
Hpy99I CGWCG 1 cut(s) 2258
HpyAV CCTTC 7 cut(s) 190, 807, 1049, 1946, 2062, 2125, 2474
HpyCH4III ACNGT 3 cut(s) 1670, 1776, 2001
HpyCH4IV ACGT 4 cut(s) 237, 492, 1582, 2563
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 959, 1814, 1993, 2042, 2258, 2290, 2430
HpyF3I CTNAG 8 cut(s) 95, 131, 767, 845, 1931, 2375, 2439, 2464
HpySE526I ACGT 4 cut(s) 237, 492, 1582, 2563
Hsp92I GRCGYC 1 cut(s) 2293
Hsp92II CATG 5 cut(s) 709, 1699, 1714, 1847, 2043
HspAI GCGC 2 cut(s) 2293, 2428
KasI GGCGCC 1 cut(s) 2292
Kpn2I TCCGGA 1 cut(s) 1040
KpnI GGTACC 2 cut(s) 1067, 2472
Ksp22I TGATCA 1 cut(s) 1254
KspAI GTTAAC 1 cut(s) 1423
LmnI GCTCC 4 cut(s) 15, 776, 1346, 2459
LweI GCATC 8 cut(s) 283, 736, 1097, 1274, 1432, 1849, 2045, 2112
MabI ACCWGGT 1 cut(s) 1066
MaeI CTAG 9 cut(s) 258, 513, 936, 1431, 1794, 1922, 1980, 2577, 2635
MaeII ACGT 4 cut(s) 237, 492, 1582, 2563
MaeIII GTNAC 5 cut(s) 493, 1478, 1664, 2086, 2185
MboII GAAGA 8 cut(s) 264, 705, 708, 1132, 1246, 1519, 2363, 2379
MfeI CAATTG 3 cut(s) 881, 1102, 2126
MflI RGATCY 4 cut(s) 572, 1522, 1551, 2200
MhlI GDGCHC 3 cut(s) 867, 1869, 1948
MlsI TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
MluNI TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
Mly113I GGCGCC 1 cut(s) 2293
MlyI GAGTC 3 cut(s) 91, 277, 451
MmeI TCCRAC 2 cut(s) 994, 1596
Mox20I TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
Mph1103I ATGCAT 2 cut(s) 1090, 1447
MroI TCCGGA 1 cut(s) 1040
MroXI GAANNNNTTC 1 cut(s) 2383
MscI TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
MseI TTAA 9 cut(s) 171, 234, 638, 969, 1422, 1499, 1566, 1799, 2337
MslI CAYNNNNRTG 1 cut(s) 1872
Msp20I TGGCCA 5 cut(s) 302, 375, 1512, 2261, 2532
MspA1I CMGCKG 2 cut(s) 1409, 1462
MspI CCGG 7 cut(s) 351, 498, 642, 656, 1041, 1531, 2514
MunI CAATTG 3 cut(s) 881, 1102, 2126
Mva1269I GAATGC 6 cut(s) 212, 949, 1445, 2410, 2500, 2572
MvaI CCWGG 6 cut(s) 1068, 1230, 1760, 2263, 2457, 2630
MwoI GCNNNNNNNGC 8 cut(s) 53, 959, 1814, 1993, 2042, 2258, 2290, 2430
NarI GGCGCC 1 cut(s) 2293
NciI CCSGG 5 cut(s) 351, 499, 643, 657, 1532
NlaIII CATG 5 cut(s) 709, 1699, 1714, 1847, 2043
NmuCI GTSAC 3 cut(s) 1478, 1664, 2185
NsiI ATGCAT 2 cut(s) 1090, 1447
NspI RCATGY 2 cut(s) 709, 1714
PaeR7I CTCGAG 1 cut(s) 2391
PagI TCATGA 1 cut(s) 1843
PceI AGGCCT 1 cut(s) 534
PciI ACATGT 1 cut(s) 705
PctI GAATGC 6 cut(s) 212, 949, 1445, 2410, 2500, 2572
PdmI GAANNNNTTC 1 cut(s) 2383
PfeI GAWTC 4 cut(s) 595, 691, 1123, 2120
PflMI CCANNNNNTGG 2 cut(s) 1013, 1409
PfoI TCCNGGA 1 cut(s) 2455
PinAI ACCGGT 1 cut(s) 2513
PleI GAGTC 3 cut(s) 90, 276, 450
PluTI GGCGCC 1 cut(s) 2296
PpsI GAGTC 3 cut(s) 90, 276, 450
Ppu21I YACGTR 1 cut(s) 2564
PpuMI RGGWCCY 2 cut(s) 1723, 2265
PscI ACATGT 1 cut(s) 705
Psp1406I AACGTT 2 cut(s) 237, 492
Psp5II RGGWCCY 2 cut(s) 1723, 2265
Psp6I CCWGG 6 cut(s) 1066, 1228, 1758, 2261, 2455, 2628
PspGI CCWGG 6 cut(s) 1066, 1228, 1758, 2261, 2455, 2628
PspPI GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1723, 2265
PspPPI RGGWCCY 2 cut(s) 1723, 2265
PstI CTGCAG 3 cut(s) 961, 1219, 1390
PstNI CAGNNNCTG 5 cut(s) 94, 162, 1385, 1462, 2268
PsuI RGATCY 4 cut(s) 572, 1522, 1551, 2200
PvuII CAGCTG 2 cut(s) 1409, 1462
RsaI GTAC 9 cut(s) 1065, 1604, 1642, 1703, 1742, 1893, 2359, 2470, 2607
RsaNI GTAC 9 cut(s) 1064, 1603, 1641, 1702, 1741, 1892, 2358, 2469, 2606
RseI CAYNNNNRTG 1 cut(s) 1872
SaqAI TTAA 9 cut(s) 171, 234, 638, 969, 1422, 1499, 1566, 1799, 2337
Sau96I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1723, 2265
SbfI CCTGCAGG 1 cut(s) 1390
ScaI AGTACT 1 cut(s) 2359
SchI GAGTC 3 cut(s) 91, 277, 451
SdaI CCTGCAGG 1 cut(s) 1390
SduI GDGCHC 3 cut(s) 867, 1869, 1948
SexAI ACCWGGT 1 cut(s) 1066
SfaNI GCATC 8 cut(s) 283, 736, 1097, 1274, 1432, 1849, 2045, 2112
SfcI CTRYAG 3 cut(s) 957, 1215, 1386
SfiI GGCCNNNNNGGCC 1 cut(s) 2258
SfoI GGCGCC 1 cut(s) 2294
Sfr274I CTCGAG 1 cut(s) 2391
SinI GGWCC 4 cut(s) 428, 541, 1723, 2265
SlaI CTCGAG 1 cut(s) 2391
SmiMI CAYNNNNRTG 1 cut(s) 1872
SmlI CTYRAG 4 cut(s) 1947, 2287, 2315, 2391
SmoI CTYRAG 4 cut(s) 1947, 2287, 2315, 2391
Sse8387I CCTGCAGG 1 cut(s) 1390
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 8 cut(s) 47, 833, 1221, 1339, 1820, 1918, 1990, 2297
SspDI GGCGCC 1 cut(s) 2292
SspI AATATT 1 cut(s) 973
SspMI CTAG 9 cut(s) 258, 513, 936, 1431, 1794, 1922, 1980, 2577, 2635
StuI AGGCCT 1 cut(s) 534
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 3 cut(s) 1670, 1776, 2001
TaiI ACGT 4 cut(s) 240, 495, 1585, 2566
TaqI TCGA 6 cut(s) 291, 674, 941, 1586, 2030, 2392
TaqII GACCGA 1 cut(s) 126
TatI WGTACW 6 cut(s) 1640, 1701, 1740, 1891, 2357, 2605
TauI GCSGC 2 cut(s) 1223, 1921
TfiI GAWTC 4 cut(s) 595, 691, 1123, 2120
Tru1I TTAA 9 cut(s) 171, 234, 638, 969, 1422, 1499, 1566, 1799, 2337
Tru9I TTAA 9 cut(s) 171, 234, 638, 969, 1422, 1499, 1566, 1799, 2337
TscAI CASTG 8 cut(s) 99, 853, 922, 961, 1673, 1894, 2113, 2122
TseFI GTSAC 3 cut(s) 1478, 1664, 2185
Tsp45I GTSAC 3 cut(s) 1478, 1664, 2185
TspDTI ATGAA 6 cut(s) 1137, 1517, 1684, 2016, 2193, 2544
TspRI CASTG 8 cut(s) 99, 853, 922, 961, 1673, 1894, 2113, 2122
Van91I CCANNNNNTGG 2 cut(s) 1013, 1409
VneI GTGCAC 1 cut(s) 1865
VpaK11BI GGWCC 4 cut(s) 428, 541, 1723, 2265
XapI RAATTY 4 cut(s) 451, 1019, 1114, 1907
XceI RCATGY 2 cut(s) 709, 1714
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 2391
XmiI GTMKAC 1 cut(s) 2566
XmnI GAANNNNTTC 1 cut(s) 2383
XspI CTAG 9 cut(s) 258, 513, 936, 1431, 1794, 1922, 1980, 2577, 2635
ZrmI AGTACT 1 cut(s) 2359
Zsp2I ATGCAT 2 cut(s) 1090, 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.