Rroxscaffold_176G00431810

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0002531
Physical Location & Seq
Reverse (-)
1623135 .. 1628765
5631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431810.1

Sequence Viewer

Length: 2535 bp
ATGGTGGATCAAAGGAGCGTAGTATGTTGTGTCTTGTTGTTGTGGGCGGTGTTGGCGTTTGAGCCCACATTTCAGCAACCGAGTCAGCAGCCACTGAGTTCACACGCCGACCGAGTTGCTCTGGTTCAGCTCAGATCGTCTTTGGGTTTGAGGGGCAGGGACTGGCCCATTAAGTCAGACCCTTGCTTCGTTTGGAAGGGTGTTGAATGCAGTAATGGTAGAGTTGTTGGGATTAACGTTTCTGGGTTTAGAAGAACTAGACTTGGGAGTCAAAACCCACAATTCTCAGTCGATGCTTTGGCCAATTTGACCCTTTTGAGTTCTTTCAATGCCTCCAATTTCTTGCTTCCGGGTTCCATTCCTGACTGGTTTGGCCAAAGGCTCGGCTCTCTGCGTGTTCTTGATCTCACTTCTTGTTCTGTGAATGGTCCTATTCCATTGAGCCTTGGAAATTCTACCAATCTGACTGGTCTGTCTTTGTCTCGTAATAACCTTACTGGGACAATTCCTGCTAGTTTGAGCAACTTGGTAGGCCTTTTGGTCCTTGACCTCTCTCAAAATCGGCTTACTGGATCCATTCCCACATCTTTCGGGAATCTTGGGAACCTTTCTGTTCTTGACATTTCTGGGAATTTCTTAACCGGGTCAATTCCTCCGCGCATTGGGAACCTCTCGAAGCTTCAATCTCTGAATCTTTCCAGCAACATGTTGTCTTCTTCCATTCCTCCTGAACTTGGGGGCCTTGATACCTTGGTTGACCTTGATCTCAGCTCCAATATATTGGTAGGTCTGCTGCCTTCGGGTTTGAGGAACTTGCAGAAAATTGTAGTTGCAGACAATTTGCTTGGTGGTTCTGTGGATGTTCTCTGGTCAATGCCTCAATTGAGTTTTCTTGATGTGGCTGGAAACAATTTCACTGGTTTGCTGCCCAATTCTAGCTTGAATGCTAATGCCACTGCTGCAGTATTCAATATTTCTCAAAATTCATTTTATGGGCCTATTACACCATTAGTTGGAAAATTTGGTGTTATTGATCTTTCAGGAAACTATTTTGAAGGTGAGATACCAGGTTATGTTGGCACCAATGCATCTCTTGACAGCAACTGTCTCCAAAATTTGAAGAACCAGAGGACTTTGGCAGAATGTTCATTGTTTTATGCTGATAGGGGTTTGAGTTTTGATAATTTTGGGCAGCCTAACTCTACTCAACCTCCTGCAGCCTCACCACCTGGGAAGAAGACGGGGAGCTACAACTCAAAGGGGGTTGGTGTGGGGTCAGTTCCTGCAGGCAGCAGTCCCCCACCAGCTGGAGCATCGGTTAACTTTTCTAGCGTAGGAGATGCATTCACATATCAGCAGCTGCTTCAAGCTACGGGTGACTTCAATAATACAAACTTAATGAAAAATGGCCACACAGGGGATCTCTTCAGGGGTGTCTTGGAAAATGGGATCCCTGTGGTTATTAAAAGGATTGATTTACGTTCGATTAAAAAGGAAGCGTACCTGCTGGAGTTGGATTTCTTTAGTAAGGTGTCAAGTATAAGATTTGTTCCCTTTCTGGGACACTGTTTAGAGGATGAGAACGAGAAGTTCATGGTGTACAAATACATGCCAAATGGGGACCTATCAAGTTCTCTGTACAAGAAAACCAACACCCTGGATGATACTTTACAGTCATTGGATTGGATCACTAGATTAAAGATCGCATTAGGTGCTGCGGAGGGTCTCACCTATCTGCATCATGAGTGCGATCCACCCCTCGTGCACAGAGATGTGCAAGCAAGTAGTGTACTTCTTGATGATAAATTTGAAGTGCGGCTAGGAAGCCTGAGTGATGTCTGTGCTCAAGAAGGAGATACTCATCAAAGCAGGATTACTAGGTTGCTGCGGTTGCCACAGTCATCCGAACAAGGTGCTTCTGGTTCATCGACAGCATCATGTGCCTATGATGTTTATTGTTTCGGGAAGGTATTACTTGAACTGGTAACAGGTAAATTGGGCATCAGTGCTTCCAGTGAATCCCAATTGAAGGAATTGTTAGATGACATACTTCCCTACATCAGTATATATGACAAGGAACTTGTGACAAAAATTGTGGATCCATCTTTGATTGTGGATGAGGACTTATTAGAGGAAGTGTGGGCTATGGCCGTTGTGGCCAGGTCCTGCCTAAATCCCAAGCCCTCAAGGCGCCCGCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTGTTAAGGTAGTAAGGGAAGATAGTACTGGCTCTGCAAGGCTCAGAAAAGCTTCTTCTCGAGGGTCTTGGAATGCTGCTGTCTTTGGTAGCTGGCGCAGCTCATCTGAGGTAGTGGTTATTCCAGGAGCTTCAGGTACCAAAGCTGAAGGAGTGAGTGGTTTGAAGCATTCAGCAACCACCGGTTCGCAGGGAAGTGGCCAAAATGGTGGGGGTGAACATTCATCCTCACGTAGACGGCATTCTAGGGATATTTTCCCTGAACCATCTGGTGTACAAGATGTAGAGAGATTTGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

844

Amino Acids

90.64

Weight (kDa)

6.02

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 130 - 276 1.5e-13 Leucine-rich repeat region
LRR_8 PF13855 154 - 211 4.2e-09 Leucine rich repeat
LRR_LRWD1 PF23211 219 - 307 9.3e-07 Leucine-rich repeat and WD repeat-containing protein 1, LRR
PK_Tyr_Ser-Thr PF07714 467 - 740 2.4e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 469 - 738 1.5e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 267, 472
Acc36I ACCTGC 1 cut(s) 1512
Acc65I GGTACC 1 cut(s) 2366
AccB1I GGYRCC 3 cut(s) 1079, 2190, 2366
AccB7I CCANNNNNTGG 2 cut(s) 1013, 1307
AccI GTMKAC 1 cut(s) 2464
AccII CGCG 1 cut(s) 658
AciI CCGC 6 cut(s) 47, 656, 1718, 1816, 1888, 2195
AclI AACGTT 1 cut(s) 237
AcoI YGGCCR 6 cut(s) 300, 373, 1408, 2148, 2157, 2428
AcsI RAATTY 6 cut(s) 451, 631, 982, 1019, 1114, 1805
AcuI CTGAAG 3 cut(s) 1411, 2346, 2397
AcyI GRCGYC 1 cut(s) 2191
AfaI GTAC 7 cut(s) 1502, 1601, 1640, 1791, 2257, 2368, 2505
AfiI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1307, 1417, 1559, 2029, 2236
AflIII ACRYGT 1 cut(s) 705
AgeI ACCGGT 1 cut(s) 2411
AjnI CCWGG 6 cut(s) 1066, 1228, 1656, 2159, 2353, 2526
Alw21I GWGCWC 2 cut(s) 1767, 1846
Alw26I GTCTC 3 cut(s) 486, 1112, 1730
Alw44I GTGCAC 1 cut(s) 1763
AlwNI CAGNNNCTG 6 cut(s) 94, 162, 1104, 1283, 1360, 2166
Ama87I CYCGRG 1 cut(s) 2289
ApaLI GTGCAC 1 cut(s) 1763
ApoI RAATTY 6 cut(s) 451, 631, 982, 1019, 1114, 1805
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AsiGI ACCGGT 1 cut(s) 2411
Asp700I GAANNNNTTC 1 cut(s) 2281
Asp718I GGTACC 1 cut(s) 2366
AspLEI GCGC 3 cut(s) 660, 2193, 2328
AspS9I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1621, 2163
AsuC2I CCSGG 2 cut(s) 351, 643
AsuHPI GGTGA 5 cut(s) 1070, 1215, 1388, 1720, 2456
AvaI CYCGRG 1 cut(s) 2289
AvaII GGWCC 4 cut(s) 428, 541, 1621, 2163
BaeGI GKGCMC 1 cut(s) 1767
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BalI TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
BamHI GGATCC 3 cut(s) 572, 1449, 2098
BanI GGYRCC 3 cut(s) 1079, 2190, 2366
BanII GRGCYC 1 cut(s) 66
BarI GAAGNNNNNNTAC 2 cut(s) 1047, 1079
BauI CACGAG 1 cut(s) 1760
BbsI GAAGAC 2 cut(s) 705, 1244
Bbv12I GWGCWC 2 cut(s) 1767, 1846
BccI CCATC 2 cut(s) 2110, 2503
BceAI ACGGC 2 cut(s) 2135, 2483
BcgI CGANNNNNNTGC 4 cut(s) 98, 132, 1895, 1929
BciT130I CCWGG 6 cut(s) 1068, 1230, 1658, 2161, 2355, 2528
BcnI CCSGG 2 cut(s) 351, 643
BcoDI GTCTC 3 cut(s) 486, 1112, 1730
BfaI CTAG 9 cut(s) 258, 513, 936, 1329, 1692, 1820, 1878, 2475, 2533
BfmI CTRYAG 3 cut(s) 960, 1215, 1284
BfoI RGCGCY 1 cut(s) 2194
BfuAI ACCTGC 1 cut(s) 1512
BglI GCCNNNNNGGC 2 cut(s) 2156, 2188
BmcAI AGTACT 1 cut(s) 2257
Bme1390I CCNGG 8 cut(s) 351, 643, 1068, 1230, 1658, 2161, 2355, 2528
Bme18I GGWCC 4 cut(s) 428, 541, 1621, 2163
BmeT110I CYCGRG 1 cut(s) 2289
BmgT120I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1621, 2163
BmrFI CCNGG 8 cut(s) 351, 643, 1068, 1230, 1658, 2161, 2355, 2528
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 7 cut(s) 283, 1097, 1322, 1330, 1747, 1943, 2010
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 705, 1244
BpmI CTGGAG 2 cut(s) 1329, 1529
BpuEI CTTGAG 3 cut(s) 1830, 2170, 2198
BpuMI CCSGG 2 cut(s) 351, 643
BsaAI YACGTR 1 cut(s) 2462
BsaBI GATNNNNATC 1 cut(s) 1860
BsaHI GRCGYC 1 cut(s) 2191
BsaI GGTCTC 1 cut(s) 1730
BsaJI CCNNGG 4 cut(s) 445, 750, 1229, 1656
BsaWI WCCGGW 1 cut(s) 2411
BsaXI ACNNNNNCTCC 4 cut(s) 1195, 1225, 1302, 1332
Bsc4I CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1307, 1417, 1559, 2029, 2236
Bse118I RCCGGY 1 cut(s) 2411
Bse1I ACTGG 9 cut(s) 167, 371, 472, 502, 574, 922, 1986, 2013, 2263
Bse8I GATNNNNATC 1 cut(s) 1860
BseBI CCWGG 6 cut(s) 1068, 1230, 1658, 2161, 2355, 2528
BseDI CCNNGG 4 cut(s) 445, 750, 1229, 1656
BseGI GGATG 6 cut(s) 865, 1582, 1666, 1901, 2122, 2453
BseJI GATNNNNATC 1 cut(s) 1860
BseLI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1307, 1417, 1559, 2029, 2236
BseMII CTCAG 7 cut(s) 86, 145, 300, 781, 1820, 2287, 2328
BseNI ACTGG 9 cut(s) 167, 371, 472, 502, 574, 922, 1986, 2013, 2263
BseSI GKGCMC 1 cut(s) 1767
Bsh1236I CGCG 1 cut(s) 658
Bsh1285I CGRYCG 1 cut(s) 112
BshNI GGYRCC 3 cut(s) 1079, 2190, 2366
BshTI ACCGGT 1 cut(s) 2411
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 1767, 1846
BsiHKCI CYCGRG 1 cut(s) 2289
BsiSI CCGG 3 cut(s) 350, 642, 2412
BslFI GGGAC 5 cut(s) 173, 514, 1281, 1575, 1634
BslI CCNNNNNNNGG 8 cut(s) 662, 734, 1013, 1307, 1417, 1559, 2029, 2236
BsmAI GTCTC 3 cut(s) 486, 1112, 1730
BsmFI GGGAC 5 cut(s) 173, 514, 1281, 1575, 1634
BsmI GAATGC 6 cut(s) 212, 949, 1343, 2308, 2398, 2470
Bso31I GGTCTC 1 cut(s) 1730
BsoBI CYCGRG 1 cut(s) 2289
Bsp1286I GDGCHC 3 cut(s) 66, 1767, 1846
Bsp1407I TGTACA 3 cut(s) 1599, 1638, 2503
BspACI CCGC 6 cut(s) 47, 656, 1718, 1816, 1888, 2195
BspCNI CTCAG 7 cut(s) 87, 144, 299, 780, 1821, 2286, 2329
BspFNI CGCG 1 cut(s) 658
BspHI TCATGA 1 cut(s) 1741
BspMAI CTGCAG 3 cut(s) 964, 1219, 1288
BspMI ACCTGC 1 cut(s) 1512
BspT107I GGYRCC 3 cut(s) 1079, 2190, 2366
BspTNI GGTCTC 1 cut(s) 1730
BsrFI RCCGGY 1 cut(s) 2411
BsrGI TGTACA 3 cut(s) 1599, 1638, 2503
BsrI ACTGG 9 cut(s) 167, 371, 472, 502, 574, 922, 1986, 2013, 2263
BssAI RCCGGY 1 cut(s) 2411
BssECI CCNNGG 4 cut(s) 445, 750, 1229, 1656
BssNI GRCGYC 1 cut(s) 2191
BssSI CACGAG 1 cut(s) 1760
BssT1I CCWWGG 2 cut(s) 445, 750
Bst2BI CACGAG 1 cut(s) 1760
Bst2UI CCWGG 6 cut(s) 1068, 1230, 1658, 2161, 2355, 2528
Bst4CI ACNGT 4 cut(s) 1106, 1568, 1674, 1899
Bst6I CTCTTC 1 cut(s) 1430
BstACI GRCGYC 1 cut(s) 2191
BstAPI GCANNNNNTGC 2 cut(s) 1712, 1940
BstAUI TGTACA 3 cut(s) 1599, 1638, 2503
BstBAI YACGTR 1 cut(s) 2462
BstC8I GCNNGC 4 cut(s) 1288, 1779, 2195, 2324
BstDEI CTNAG 7 cut(s) 95, 131, 286, 767, 1829, 2273, 2337
BstF5I GGATG 6 cut(s) 865, 1582, 1666, 1901, 2122, 2453
BstFNI CGCG 1 cut(s) 658
BstH2I RGCGCY 1 cut(s) 2194
BstHHI GCGC 3 cut(s) 660, 2193, 2328
BstMAI GTCTC 3 cut(s) 486, 1112, 1730
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 8 cut(s) 53, 959, 1712, 1891, 1940, 2156, 2188, 2328
BstNI CCWGG 6 cut(s) 1068, 1230, 1658, 2161, 2355, 2528
BstNSI RCATGY 2 cut(s) 709, 1612
BstSCI CCNGG 8 cut(s) 349, 641, 1066, 1228, 1656, 2159, 2353, 2526
BstSFI CTRYAG 3 cut(s) 960, 1215, 1284
BstSLI GKGCMC 1 cut(s) 1767
BstUI CGCG 1 cut(s) 658
BstV2I GAAGAC 2 cut(s) 705, 1244
BstX2I RGATCY 4 cut(s) 572, 1420, 1449, 2098
BstXI CCANNNNNNTGG 3 cut(s) 781, 1657, 2438
BstYI RGATCY 4 cut(s) 572, 1420, 1449, 2098
BtsCI GGATG 6 cut(s) 865, 1582, 1666, 1901, 2122, 2453
BtsI GCAGTG 1 cut(s) 954
BtsIMutI CAGTG 6 cut(s) 92, 915, 954, 1564, 2011, 2020
BveI ACCTGC 1 cut(s) 1512
Cac8I GCNNGC 4 cut(s) 1288, 1779, 2195, 2324
CaiI CAGNNNCTG 6 cut(s) 94, 162, 1104, 1283, 1360, 2166
CciI TCATGA 1 cut(s) 1741
CfoI GCGC 3 cut(s) 660, 2193, 2328
Cfr10I RCCGGY 1 cut(s) 2411
Cfr13I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1621, 2163
CsiI ACCWGGT 1 cut(s) 1066
Csp6I GTAC 7 cut(s) 1501, 1600, 1639, 1790, 2256, 2367, 2504
CspAI ACCGGT 1 cut(s) 2411
CspCI CAANNNNNGTGG 2 cut(s) 2076, 2111
CviAII CATG 5 cut(s) 706, 1594, 1609, 1742, 1938
CviQI GTAC 7 cut(s) 1501, 1600, 1639, 1790, 2256, 2367, 2504
DdeI CTNAG 7 cut(s) 95, 131, 286, 767, 1829, 2273, 2337
DinI GGCGCC 1 cut(s) 2192
DrdI GACNNNNNNGTC 2 cut(s) 267, 472
DseDI GACNNNNNNGTC 2 cut(s) 267, 472
EaeI YGGCCR 6 cut(s) 300, 373, 1408, 2148, 2157, 2428
Eam1104I CTCTTC 1 cut(s) 1430
EarI CTCTTC 1 cut(s) 1430
Eco130I CCWWGG 2 cut(s) 445, 750
Eco147I AGGCCT 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 1730
Eco47I GGWCC 4 cut(s) 428, 541, 1621, 2163
Eco57I CTGAAG 3 cut(s) 1411, 2346, 2397
Eco88I CYCGRG 1 cut(s) 2289
EcoO109I RGGNCCY 3 cut(s) 739, 1621, 2163
EcoRII CCWGG 6 cut(s) 1066, 1228, 1656, 2159, 2353, 2526
EcoT14I CCWWGG 2 cut(s) 445, 750
EcoT22I ATGCAT 2 cut(s) 1090, 1345
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 2192
EheI GGCGCC 1 cut(s) 2192
ErhI CCWWGG 2 cut(s) 445, 750
FaeI CATG 5 cut(s) 709, 1597, 1612, 1745, 1941
FaqI GGGAC 5 cut(s) 173, 514, 1281, 1575, 1634
FatI CATG 5 cut(s) 705, 1593, 1608, 1741, 1937
FauI CCCGC 1 cut(s) 2202
FblI GTMKAC 1 cut(s) 2464
FokI GGATG 6 cut(s) 872, 1589, 1673, 1888, 2129, 2440
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 9 cut(s) 258, 513, 936, 1329, 1692, 1820, 1878, 2475, 2533
GlaI GCGC 3 cut(s) 659, 2192, 2327
GsuI CTGGAG 2 cut(s) 1329, 1529
HaeII RGCGCY 1 cut(s) 2194
HapII CCGG 3 cut(s) 350, 642, 2412
HhaI GCGC 3 cut(s) 660, 2193, 2328
Hin1I GRCGYC 1 cut(s) 2191
Hin1II CATG 5 cut(s) 709, 1597, 1612, 1745, 1941
Hin6I GCGC 3 cut(s) 658, 2191, 2326
HinP1I GCGC 3 cut(s) 658, 2191, 2326
HincII GTYRAC 2 cut(s) 757, 1321
HindII GTYRAC 2 cut(s) 757, 1321
HindIII AAGCTT 2 cut(s) 677, 2280
HinfI GANTC 5 cut(s) 82, 268, 595, 691, 2018
HpaI GTTAAC 1 cut(s) 1321
HpaII CCGG 3 cut(s) 350, 642, 2412
HphI GGTGA 5 cut(s) 1070, 1215, 1388, 1720, 2456
Hpy166II GTNNAC 9 cut(s) 101, 757, 1321, 1600, 1765, 1790, 2447, 2465, 2504
Hpy188I TCNGA 7 cut(s) 134, 178, 465, 690, 1906, 2276, 2338
Hpy8I GTNNAC 9 cut(s) 101, 757, 1321, 1600, 1765, 1790, 2447, 2465, 2504
HpyAV CCTTC 7 cut(s) 190, 807, 1049, 1844, 1960, 2023, 2372
HpyCH4III ACNGT 4 cut(s) 1106, 1568, 1674, 1899
HpyCH4IV ACGT 3 cut(s) 237, 1480, 2461
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 959, 1712, 1891, 1940, 2156, 2188, 2328
HpyF3I CTNAG 7 cut(s) 95, 131, 286, 767, 1829, 2273, 2337
HpySE526I ACGT 3 cut(s) 237, 1480, 2461
Hsp92I GRCGYC 1 cut(s) 2191
Hsp92II CATG 5 cut(s) 709, 1597, 1612, 1745, 1941
HspAI GCGC 3 cut(s) 658, 2191, 2326
KasI GGCGCC 1 cut(s) 2190
KpnI GGTACC 1 cut(s) 2370
KspAI GTTAAC 1 cut(s) 1321
LmnI GCTCC 5 cut(s) 15, 776, 1245, 1310, 2357
LweI GCATC 7 cut(s) 283, 1097, 1322, 1330, 1747, 1943, 2010
MabI ACCWGGT 1 cut(s) 1066
MaeI CTAG 9 cut(s) 258, 513, 936, 1329, 1692, 1820, 1878, 2475, 2533
MaeII ACGT 3 cut(s) 237, 1480, 2461
MaeIII GTNAC 3 cut(s) 1376, 1984, 2083
MboII GAAGA 9 cut(s) 264, 705, 708, 1132, 1246, 1249, 1417, 2261, 2277
MfeI CAATTG 2 cut(s) 881, 2024
MflI RGATCY 4 cut(s) 572, 1420, 1449, 2098
MhlI GDGCHC 3 cut(s) 66, 1767, 1846
MlsI TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
MluNI TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
Mly113I GGCGCC 1 cut(s) 2191
MlyI GAGTC 2 cut(s) 91, 277
MmeI TCCRAC 2 cut(s) 994, 1494
Mox20I TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
Mph1103I ATGCAT 2 cut(s) 1090, 1345
MroXI GAANNNNTTC 1 cut(s) 2281
MscI TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
MseI TTAA 9 cut(s) 171, 234, 638, 1320, 1397, 1464, 1488, 1697, 2235
MslI CAYNNNNRTG 1 cut(s) 1770
Msp20I TGGCCA 5 cut(s) 302, 375, 1410, 2159, 2430
MspA1I CMGCKG 2 cut(s) 1307, 1360
MspI CCGG 3 cut(s) 350, 642, 2412
MspR9I CCNGG 8 cut(s) 351, 643, 1068, 1230, 1658, 2161, 2355, 2528
MunI CAATTG 2 cut(s) 881, 2024
Mva1269I GAATGC 6 cut(s) 212, 949, 1343, 2308, 2398, 2470
MvaI CCWGG 6 cut(s) 1068, 1230, 1658, 2161, 2355, 2528
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 8 cut(s) 53, 959, 1712, 1891, 1940, 2156, 2188, 2328
NarI GGCGCC 1 cut(s) 2191
NciI CCSGG 2 cut(s) 351, 643
NlaIII CATG 5 cut(s) 709, 1597, 1612, 1745, 1941
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 1376, 2083
NsiI ATGCAT 2 cut(s) 1090, 1345
NspI RCATGY 2 cut(s) 709, 1612
PaeR7I CTCGAG 1 cut(s) 2289
PagI TCATGA 1 cut(s) 1741
PceI AGGCCT 1 cut(s) 534
PciI ACATGT 1 cut(s) 705
PctI GAATGC 6 cut(s) 212, 949, 1343, 2308, 2398, 2470
PdmI GAANNNNTTC 1 cut(s) 2281
PfeI GAWTC 3 cut(s) 595, 691, 2018
PflMI CCANNNNNTGG 2 cut(s) 1013, 1307
PfoI TCCNGGA 1 cut(s) 2353
PinAI ACCGGT 1 cut(s) 2411
PleI GAGTC 2 cut(s) 90, 276
PluTI GGCGCC 1 cut(s) 2194
PpsI GAGTC 2 cut(s) 90, 276
Ppu21I YACGTR 1 cut(s) 2462
PpuMI RGGWCCY 2 cut(s) 1621, 2163
PscI ACATGT 1 cut(s) 705
Psp1406I AACGTT 1 cut(s) 237
Psp5II RGGWCCY 2 cut(s) 1621, 2163
Psp6I CCWGG 6 cut(s) 1066, 1228, 1656, 2159, 2353, 2526
PspGI CCWGG 6 cut(s) 1066, 1228, 1656, 2159, 2353, 2526
PspPI GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1621, 2163
PspPPI RGGWCCY 2 cut(s) 1621, 2163
PstI CTGCAG 3 cut(s) 964, 1219, 1288
PstNI CAGNNNCTG 6 cut(s) 94, 162, 1104, 1283, 1360, 2166
PsuI RGATCY 4 cut(s) 572, 1420, 1449, 2098
PvuII CAGCTG 2 cut(s) 1307, 1360
RsaI GTAC 7 cut(s) 1502, 1601, 1640, 1791, 2257, 2368, 2505
RsaNI GTAC 7 cut(s) 1501, 1600, 1639, 1790, 2256, 2367, 2504
RseI CAYNNNNRTG 1 cut(s) 1770
SaqAI TTAA 9 cut(s) 171, 234, 638, 1320, 1397, 1464, 1488, 1697, 2235
Sau96I GGNCC 7 cut(s) 165, 428, 541, 739, 995, 1621, 2163
SbfI CCTGCAGG 1 cut(s) 1288
ScaI AGTACT 1 cut(s) 2257
SchI GAGTC 2 cut(s) 91, 277
ScrFI CCNGG 8 cut(s) 351, 643, 1068, 1230, 1658, 2161, 2355, 2528
SdaI CCTGCAGG 1 cut(s) 1288
SduI GDGCHC 3 cut(s) 66, 1767, 1846
SexAI ACCWGGT 1 cut(s) 1066
SfaNI GCATC 7 cut(s) 283, 1097, 1322, 1330, 1747, 1943, 2010
SfcI CTRYAG 3 cut(s) 960, 1215, 1284
SfiI GGCCNNNNNGGCC 1 cut(s) 2156
SfoI GGCGCC 1 cut(s) 2192
Sfr274I CTCGAG 1 cut(s) 2289
SinI GGWCC 4 cut(s) 428, 541, 1621, 2163
SlaI CTCGAG 1 cut(s) 2289
SmiMI CAYNNNNRTG 1 cut(s) 1770
SmlI CTYRAG 4 cut(s) 1845, 2185, 2213, 2289
SmoI CTYRAG 4 cut(s) 1845, 2185, 2213, 2289
Sse8387I CCTGCAGG 1 cut(s) 1288
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 6 cut(s) 47, 656, 1718, 1816, 1888, 2195
SspDI GGCGCC 1 cut(s) 2190
SspI AATATT 1 cut(s) 973
SspMI CTAG 9 cut(s) 258, 513, 936, 1329, 1692, 1820, 1878, 2475, 2533
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 8 cut(s) 349, 641, 1066, 1228, 1656, 2159, 2353, 2526
StyI CCWWGG 2 cut(s) 445, 750
TaaI ACNGT 4 cut(s) 1106, 1568, 1674, 1899
TaiI ACGT 3 cut(s) 240, 1483, 2464
TaqI TCGA 5 cut(s) 291, 674, 1484, 1928, 2290
TaqII GACCGA 1 cut(s) 126
TatI WGTACW 5 cut(s) 1599, 1638, 1789, 2255, 2503
TauI GCSGC 1 cut(s) 1819
TfiI GAWTC 3 cut(s) 595, 691, 2018
Tru1I TTAA 9 cut(s) 171, 234, 638, 1320, 1397, 1464, 1488, 1697, 2235
Tru9I TTAA 9 cut(s) 171, 234, 638, 1320, 1397, 1464, 1488, 1697, 2235
TscAI CASTG 6 cut(s) 99, 922, 961, 1571, 2011, 2020
TseFI GTSAC 2 cut(s) 1376, 2083
Tsp45I GTSAC 2 cut(s) 1376, 2083
TspDTI ATGAA 6 cut(s) 975, 1137, 1415, 1582, 1914, 2442
TspRI CASTG 6 cut(s) 99, 922, 961, 1571, 2011, 2020
Van91I CCANNNNNTGG 2 cut(s) 1013, 1307
VneI GTGCAC 1 cut(s) 1763
VpaK11BI GGWCC 4 cut(s) 428, 541, 1621, 2163
XapI RAATTY 6 cut(s) 451, 631, 982, 1019, 1114, 1805
XceI RCATGY 2 cut(s) 709, 1612
XhoI CTCGAG 1 cut(s) 2289
XmiI GTMKAC 1 cut(s) 2464
XmnI GAANNNNTTC 1 cut(s) 2281
XspI CTAG 9 cut(s) 258, 513, 936, 1329, 1692, 1820, 1878, 2475, 2533
ZrmI AGTACT 1 cut(s) 2257
Zsp2I ATGCAT 2 cut(s) 1090, 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.