Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002531

Physical Location & Seq

Genomic Coordinates Reverse (-)

20294201 .. 20299265

Gene Structure 5065 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G011550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGGTGGATCAAAGGAGCATGGTATGGTGTTTCTTGTTGTTGTGGGCGGTGTTGGTGTTTGAGCCCACATTTCAGCAACCGAGTCAGCAGCCACTGAGTTCACACGCCGACCGAGTTGCTCTGGTTCAGCTCAGATCGTCTTTGGGTTTGAGGGGCAGGGATTGGCCCATTAAGTCGGACCCTTGCTTCGTTTGGAAGGGTGTTGAATGCAGTAATGGTAGAGTTGTTGGGATTAACGTTTCTGGGTTTAGAAGAACTAGACTTGGGAGTCAAAACCCACAGTTTTCAGTCGATGCTTTGGCCAATTTGACCCTTTTGAGTTCTTTCAATGCCTCCAATTTCTTGCTTCCGGGTTCCATTCCTGACTGGTTTGGCCAGAGGCTCGGGTCTCTGCGTGTTCTTGATCTCACTTCTTGTTCTGTGAATGGTCCTATTCCATTGAGTCTTGGAAATTCTACCAATCTGACTGGTCTGTCTTTGTCTCGTAATAACCTTAATGGGACAATTCCTGCTAGTTTGAGCAACTTGGTAAGCCTTTTGGTCCTTGACCTCTCTCAAAATCGGCTTACTGGATCCATTCCCACATCTTTCGGGAATCTTGGGAACCTTTCGGTTCTTGACATTTCTGGGAATTACTTAACCGGGTCAATTCCTCCGGGCATTGGGAACCTCTTGAAGCTTCAATCTCTGAATCTTTCCAGCAACATGTTGTCTTCTTCCATTCCTCCTGAACTTGGGGGCCTTGATACCTTGGTTGACCTTGATCTCAGCTCCAATCTATTGGTAGGTCTGCTGCCTTCGGTTTTCTTGATGTGGCTGGAAACAATTTCACTGGTTTGCTGCCCAATTAGCTCGAATACTAATGCCACTGCGGCAGTATTCAATATTTCTCTAAATTCGTTTTATGGGCCTATTACACCATTAGTTGGAAAGTTTGGTGTCATTGATCTTTCAGGAAACTATTTTGAAGGTGAGATACCAGGTTATGTTGGCACCAATGCATCTCTTGACAGCAATTGTCTCCAAAATTTGAAGAATCAGAGGACTTTGGCAGAATGTCTCATCCCAAACTCTACACAACCTCCTGCAGCCGCACCACCTGGGAAGAAAAGTAATAAAAATGTGATCATATTGGCATCAGTTTTAGGAGGATTTGGTTTGATTGTGGTTTTGGTCTTGTTTTTAGTAATAGTAGTCCTATGTTCACGCAAGCGGGGAGCTACAACTCAAAGGGGGGTTGGTGTGGGGTCATCGGTTAACTTTTCTAGCGTAGGAGATGCATTCACATATCAGCAGCTGCTTCAAGCTACAGGTGACTTCAACAATACAAACTTAATGAAAAATGGCCACACAGGGGATCTCTTCAGGGGTGTCTTGGAAAATGGGATCCCTGTGGTTATTAAAAGGATTGATTTACGTTCGATTAAAAAGGAAGCGTACTTGCTGGAGTTGGATTTCTTTAGTAAGGTGTCAAGTACAAGATTTGTTCCCTTTCTGGGACACTGTTTAGAGAATGAGAACGAGAAGTTCATGGTGTACAAATACATGCCAAATGGGGACCTATCAAATTCTCTGTACAAGAAAACCAACACCCTGGATGATACTTTACAGTCATTGGATTGGATCACTAGATTAAAGATTGCATTAGGTGCTGCGGAGGGTCTCACCTATCTGCATCATGAGTGTGATCCACCCCTCGTGCACAGTGCGGCTAGGAAGCTGAGTGATGTCTGTGCTCAAGAAGGAGATACTCATCAAAGCAGGATTACTAGGTTGCTGCGGTTGCCACAGTCATCCGAACAAGGTGCTTCTGGTTCATCGACAGCATCATGTGCCTATGATGTTTATTGTTTCGGGAAGGTATTACTTGAACTGGTAACAGGTAAATTGGGCATCAGTGCTTCCAGTGAATCCCAATTGAAGGAATCGTTGGATGACATACTTCCCTACATCAGTATATATGACAAGGAACTTGTGACAAAAATTGTGGATCCATCTTTAATTGTGGATGAGGACTTATTAGAGGAAGTGTGGGCTATGGCCGTCGTGGCCAGGTCCTGCCTAAATCCCAAGCCCTCAAGGCGCCCGCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTGTTAAGGTAGTAAGGGAAGAGAGTACTGGCTCTGCAAGGCTCAGAAAAGCTTCTTCTCGAGGGTCTTGGAATGCTGCTGTCTTTGGTAGCTGGCGCAGCTCATCTGAGGTAGTGGTTATTCCAGGAGCCTCAGGTACCAAAGCTGAAGGAGTGAGTGGTTTGAAGCATTCAGCAACCACCGGTTCGCAGGGAAGTGGCCAAAATGGTGGGGGTGAACATTCATCCTCACGTAGACGGCATTCTAGGGATATTTTCCCTGAACCATCTGGTGTACAAGATGTAGAGAGATTTGACCAGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

808

Amino Acids

87.21

Weight (kDa)

7.84

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	130 - 257	2.8e-13	Leucine-rich repeat region
LRR_8	PF13855	154 - 213	6.3e-09	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	446 - 561	3.4e-11	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002531)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16250 AT2G16250
fragaria_vesca	FvH4_2g08850 FvH4_2g39760 FvH4_2g39760 FvH4_2g39760 FvH4_2g39760
malus_domestica	MD05G1065800.v1.1 MD08G1031300.v1.1 MD10G1075800.v1.1 MD15G1027100.v1.1
prunus_persica	Prupe.1G378900_v2.0.a1 Prupe.8G096500_v2.0.a1 Prupe.8G096500_v2.0.a1 Prupe.8G096500_v2.0.a1 Prupe.8G096500_v2.0.a1
pyrus_communis	pycom05g05720 pycom08g02470 pycom10g05940 pycom15g02310
rosa_chinensis	RchiOBHm_Chr6g0264451 RchiOBHm_Chr6g0301101
rosa_laevigata	RLG00000011278 RLG00000014252
rosa_multiflora	Rmu_co8095654.1_g000001 Rmu_sc0004470.1_g000003
rosa_roxburghii	Rroxscaffold_172G00445540 Rroxscaffold_176G00431810 Rroxscaffold_7G00167160
rosa_rugosa	Rorug06G0012700 Rorug06G0012800 Rorug06G0301300
rosa_samantha	Rh6AG132200 Rh6AG414700 Rh6BG129500 Rh6BG421700 Rh6CG127700 Rh6CG429300 Rh6DG115000 Rh6DG414600
rosa_wichuraiana	Rw6G011550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	267, 472
Acc65I	GGTACC	1 cut(s)	2258
AccB1I	GGYRCC	3 cut(s)	992, 2082, 2258
AccB7I	CCANNNNNTGG	1 cut(s)	926
AccI	GTMKAC	1 cut(s)	2356
AciI	CCGC	8 cut(s)	47, 872, 1092, 1213, 1655, 1709, 1780, 2087
AclI	AACGTT	1 cut(s)	237
AcoI	YGGCCR	6 cut(s)	300, 373, 1345, 2040, 2049, 2320
AcsI	RAATTY	4 cut(s)	451, 895, 1027, 1567
AcuI	CTGAAG	2 cut(s)	1348, 2289
AcyI	GRCGYC	1 cut(s)	2083
AfaI	GTAC	7 cut(s)	1439, 1477, 1538, 1577, 2149, 2260, 2397
AfiI	CCNNNNNNNGG	7 cut(s)	662, 734, 926, 1354, 1496, 1921, 2128
AflIII	ACRYGT	1 cut(s)	705
AgeI	ACCGGT	1 cut(s)	2303
AjnI	CCWGG	6 cut(s)	979, 1099, 1593, 2051, 2245, 2418
AjuI	GAANNNNNNNTTGG	2 cut(s)	1913, 1945
Alw21I	GWGCWC	2 cut(s)	1704, 1738
Alw26I	GTCTC	5 cut(s)	393, 486, 1025, 1064, 1667
Alw44I	GTGCAC	1 cut(s)	1700
AlwNI	CAGNNNCTG	3 cut(s)	94, 1297, 2058
Ama87I	CYCGRG	2 cut(s)	383, 2181
AoxI	GGCC	9 cut(s)	164, 300, 373, 739, 908, 1345, 2040, 2049, 2320
ApaLI	GTGCAC	1 cut(s)	1700
ApoI	RAATTY	4 cut(s)	451, 895, 1027, 1567
ArsI	GACNNNNNNTTYG	2 cut(s)	170, 202
AsiGI	ACCGGT	1 cut(s)	2303
Asp700I	GAANNNNTTC	1 cut(s)	2173
Asp718I	GGTACC	1 cut(s)	2258
AspLEI	GCGC	2 cut(s)	2085, 2220
AspS9I	GGNCC	8 cut(s)	165, 178, 428, 541, 739, 908, 1558, 2055
AsuC2I	CCSGG	3 cut(s)	351, 643, 657
AsuHPI	GGTGA	4 cut(s)	983, 1325, 1657, 2348
AvaI	CYCGRG	2 cut(s)	383, 2181
AvaII	GGWCC	5 cut(s)	178, 428, 541, 1558, 2055
AxyI	CCTNAGG	1 cut(s)	2254
BaeGI	GKGCMC	1 cut(s)	1704
BaeI	ACNNNNGTAYC	2 cut(s)	738, 771
BalI	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
BamHI	GGATCC	3 cut(s)	572, 1386, 1990
BanI	GGYRCC	3 cut(s)	992, 2082, 2258
BanII	GRGCYC	1 cut(s)	66
BarI	GAAGNNNNNNTAC	2 cut(s)	960, 992
BauI	CACGAG	1 cut(s)	1697
BbsI	GAAGAC	1 cut(s)	705
Bbv12I	GWGCWC	2 cut(s)	1704, 1738
BccI	CCATC	2 cut(s)	2002, 2395
BceAI	ACGGC	2 cut(s)	2027, 2375
BcgI	CGANNNNNNTGC	4 cut(s)	98, 132, 1787, 1821
BciT130I	CCWGG	6 cut(s)	981, 1101, 1595, 2053, 2247, 2420
BclI	TGATCA	1 cut(s)	1125
BcnI	CCSGG	3 cut(s)	351, 643, 657
BcoDI	GTCTC	5 cut(s)	393, 486, 1025, 1064, 1667
BfaI	CTAG	8 cut(s)	258, 513, 1266, 1629, 1713, 1770, 2367, 2425
BfmI	CTRYAG	2 cut(s)	1086, 1308
BfoI	RGCGCY	1 cut(s)	2086
BglI	GCCNNNNNGGC	3 cut(s)	872, 2048, 2080
BmcAI	AGTACT	1 cut(s)	2149
Bme1390I	CCNGG	9 cut(s)	351, 643, 657, 981, 1101, 1595, 2053, 2247, 2420
Bme18I	GGWCC	5 cut(s)	178, 428, 541, 1558, 2055
BmeT110I	CYCGRG	2 cut(s)	383, 2181
BmgT120I	GGNCC	8 cut(s)	165, 178, 428, 541, 739, 908, 1558, 2055
BmrFI	CCNGG	9 cut(s)	351, 643, 657, 981, 1101, 1595, 2053, 2247, 2420
BmsI	GCATC	7 cut(s)	283, 1010, 1145, 1267, 1684, 1835, 1902
BpiI	GAAGAC	1 cut(s)	705
BpmI	CTGGAG	1 cut(s)	1466
BpuEI	CTTGAG	3 cut(s)	1722, 2062, 2090
BpuMI	CCSGG	3 cut(s)	351, 643, 657
BsaAI	YACGTR	1 cut(s)	2354
BsaBI	GATNNNNATC	1 cut(s)	1752
BsaHI	GRCGYC	1 cut(s)	2083
BsaI	GGTCTC	2 cut(s)	393, 1667
BsaJI	CCNNGG	3 cut(s)	750, 1100, 1593
BsaWI	WCCGGW	1 cut(s)	2303
BsaXI	ACNNNNNCTCC	2 cut(s)	1066, 1096
Bsc4I	CCNNNNNNNGG	7 cut(s)	662, 734, 926, 1354, 1496, 1921, 2128
Bse118I	RCCGGY	1 cut(s)	2303
Bse1I	ACTGG	7 cut(s)	371, 472, 574, 837, 1878, 1905, 2155
Bse21I	CCTNAGG	1 cut(s)	2254
Bse8I	GATNNNNATC	1 cut(s)	1752
BseBI	CCWGG	6 cut(s)	981, 1101, 1595, 2053, 2247, 2420
BseDI	CCNNGG	3 cut(s)	750, 1100, 1593
BseGI	GGATG	6 cut(s)	1062, 1603, 1793, 1939, 2014, 2345
BseJI	GATNNNNATC	1 cut(s)	1752
BseLI	CCNNNNNNNGG	7 cut(s)	662, 734, 926, 1354, 1496, 1921, 2128
BseMII	CTCAG	7 cut(s)	86, 145, 781, 1712, 2179, 2220, 2268
BseNI	ACTGG	7 cut(s)	371, 472, 574, 837, 1878, 1905, 2155
BseSI	GKGCMC	1 cut(s)	1704
Bsh1285I	CGRYCG	1 cut(s)	112
BshFI	GGCC	9 cut(s)	166, 302, 375, 741, 910, 1347, 2042, 2051, 2322
BshNI	GGYRCC	3 cut(s)	992, 2082, 2258
BshTI	ACCGGT	1 cut(s)	2303
BsiEI	CGRYCG	1 cut(s)	112
BsiHKAI	GWGCWC	2 cut(s)	1704, 1738
BsiHKCI	CYCGRG	2 cut(s)	383, 2181
BsiSI	CCGG	4 cut(s)	350, 642, 656, 2304
BslFI	GGGAC	3 cut(s)	514, 1512, 1571
BslI	CCNNNNNNNGG	7 cut(s)	662, 734, 926, 1354, 1496, 1921, 2128
BsmAI	GTCTC	5 cut(s)	393, 486, 1025, 1064, 1667
BsmFI	GGGAC	3 cut(s)	514, 1512, 1571
BsmI	GAATGC	5 cut(s)	212, 1280, 2200, 2290, 2362
BsnI	GGCC	9 cut(s)	166, 302, 375, 741, 910, 1347, 2042, 2051, 2322
Bso31I	GGTCTC	2 cut(s)	393, 1667
BsoBI	CYCGRG	2 cut(s)	383, 2181
Bsp1286I	GDGCHC	3 cut(s)	66, 1704, 1738
Bsp1407I	TGTACA	3 cut(s)	1536, 1575, 2395
BspACI	CCGC	8 cut(s)	47, 872, 1092, 1213, 1655, 1709, 1780, 2087
BspANI	GGCC	9 cut(s)	166, 302, 375, 741, 910, 1347, 2042, 2051, 2322
BspCNI	CTCAG	7 cut(s)	87, 144, 780, 1713, 2178, 2221, 2267
BspHI	TCATGA	1 cut(s)	1678
BspMAI	CTGCAG	1 cut(s)	1090
BspT107I	GGYRCC	3 cut(s)	992, 2082, 2258
BspTNI	GGTCTC	2 cut(s)	393, 1667
BsrFI	RCCGGY	1 cut(s)	2303
BsrGI	TGTACA	3 cut(s)	1536, 1575, 2395
BsrI	ACTGG	7 cut(s)	371, 472, 574, 837, 1878, 1905, 2155
BssAI	RCCGGY	1 cut(s)	2303
BssECI	CCNNGG	3 cut(s)	750, 1100, 1593
BssNI	GRCGYC	1 cut(s)	2083
BssSI	CACGAG	1 cut(s)	1697
BssT1I	CCWWGG	1 cut(s)	750
Bst2BI	CACGAG	1 cut(s)	1697
Bst2UI	CCWGG	6 cut(s)	981, 1101, 1595, 2053, 2247, 2420
Bst4CI	ACNGT	5 cut(s)	282, 1505, 1611, 1706, 1791
Bst6I	CTCTTC	2 cut(s)	1367, 2136
BstACI	GRCGYC	1 cut(s)	2083
BstAPI	GCANNNNNTGC	2 cut(s)	1649, 1832
BstAUI	TGTACA	3 cut(s)	1536, 1575, 2395
BstBAI	YACGTR	1 cut(s)	2354
BstC8I	GCNNGC	3 cut(s)	1211, 2087, 2216
BstDEI	CTNAG	7 cut(s)	95, 131, 767, 1721, 2165, 2229, 2254
BstF5I	GGATG	6 cut(s)	1062, 1603, 1793, 1939, 2014, 2345
BstH2I	RGCGCY	1 cut(s)	2086
BstHHI	GCGC	2 cut(s)	2085, 2220
BstMAI	GTCTC	5 cut(s)	393, 486, 1025, 1064, 1667
BstMCI	CGRYCG	1 cut(s)	112
BstMWI	GCNNNNNNNGC	8 cut(s)	849, 872, 1649, 1783, 1832, 2048, 2080, 2220
BstNI	CCWGG	6 cut(s)	981, 1101, 1595, 2053, 2247, 2420
BstNSI	RCATGY	2 cut(s)	709, 1549
BstSCI	CCNGG	9 cut(s)	349, 641, 655, 979, 1099, 1593, 2051, 2245, 2418
BstSFI	CTRYAG	2 cut(s)	1086, 1308
BstSLI	GKGCMC	1 cut(s)	1704
BstV2I	GAAGAC	1 cut(s)	705
BstX2I	RGATCY	4 cut(s)	572, 1357, 1386, 1990
BstXI	CCANNNNNNTGG	3 cut(s)	781, 1594, 2330
BstYI	RGATCY	4 cut(s)	572, 1357, 1386, 1990
Bsu36I	CCTNAGG	1 cut(s)	2254
BsuRI	GGCC	9 cut(s)	166, 302, 375, 741, 910, 1347, 2042, 2051, 2322
BtsCI	GGATG	6 cut(s)	1062, 1603, 1793, 1939, 2014, 2345
BtsI	GCAGTG	1 cut(s)	867
BtsIMutI	CAGTG	7 cut(s)	92, 830, 867, 1501, 1711, 1903, 1912
Cac8I	GCNNGC	3 cut(s)	1211, 2087, 2216
CaiI	CAGNNNCTG	3 cut(s)	94, 1297, 2058
CciI	TCATGA	1 cut(s)	1678
CfoI	GCGC	2 cut(s)	2085, 2220
Cfr10I	RCCGGY	1 cut(s)	2303
Cfr13I	GGNCC	8 cut(s)	165, 178, 428, 541, 739, 908, 1558, 2055
CsiI	ACCWGGT	1 cut(s)	979
Csp6I	GTAC	7 cut(s)	1438, 1476, 1537, 1576, 2148, 2259, 2396
CspAI	ACCGGT	1 cut(s)	2303
CspCI	CAANNNNNGTGG	2 cut(s)	1968, 2003
CviAII	CATG	6 cut(s)	19, 706, 1531, 1546, 1679, 1830
CviQI	GTAC	7 cut(s)	1438, 1476, 1537, 1576, 2148, 2259, 2396
DdeI	CTNAG	7 cut(s)	95, 131, 767, 1721, 2165, 2229, 2254
DinI	GGCGCC	1 cut(s)	2084
DrdI	GACNNNNNNGTC	2 cut(s)	267, 472
DseDI	GACNNNNNNGTC	2 cut(s)	267, 472
EaeI	YGGCCR	6 cut(s)	300, 373, 1345, 2040, 2049, 2320
Eam1104I	CTCTTC	2 cut(s)	1367, 2136
EarI	CTCTTC	2 cut(s)	1367, 2136
Eco130I	CCWWGG	1 cut(s)	750
Eco24I	GRGCYC	1 cut(s)	66
Eco31I	GGTCTC	2 cut(s)	393, 1667
Eco47I	GGWCC	5 cut(s)	178, 428, 541, 1558, 2055
Eco57I	CTGAAG	2 cut(s)	1348, 2289
Eco81I	CCTNAGG	1 cut(s)	2254
Eco88I	CYCGRG	2 cut(s)	383, 2181
EcoO109I	RGGNCCY	3 cut(s)	739, 1558, 2055
EcoRII	CCWGG	6 cut(s)	979, 1099, 1593, 2051, 2245, 2418
EcoT14I	CCWWGG	1 cut(s)	750
EcoT22I	ATGCAT	2 cut(s)	1003, 1282
EcoT38I	GRGCYC	1 cut(s)	66
EgeI	GGCGCC	1 cut(s)	2084
EheI	GGCGCC	1 cut(s)	2084
ErhI	CCWWGG	1 cut(s)	750
FaeI	CATG	6 cut(s)	22, 709, 1534, 1549, 1682, 1833
FaqI	GGGAC	3 cut(s)	514, 1512, 1571
FatI	CATG	6 cut(s)	18, 705, 1530, 1545, 1678, 1829
FauI	CCCGC	2 cut(s)	1206, 2094
FbaI	TGATCA	1 cut(s)	1125
FblI	GTMKAC	1 cut(s)	2356
FokI	GGATG	6 cut(s)	1049, 1610, 1780, 1946, 2021, 2332
FriOI	GRGCYC	1 cut(s)	66
FspBI	CTAG	8 cut(s)	258, 513, 1266, 1629, 1713, 1770, 2367, 2425
GlaI	GCGC	2 cut(s)	2084, 2219
GsuI	CTGGAG	1 cut(s)	1466
HaeII	RGCGCY	1 cut(s)	2086
HaeIII	GGCC	9 cut(s)	166, 302, 375, 741, 910, 1347, 2042, 2051, 2322
HapII	CCGG	4 cut(s)	350, 642, 656, 2304
HhaI	GCGC	2 cut(s)	2085, 2220
Hin1I	GRCGYC	1 cut(s)	2083
Hin1II	CATG	6 cut(s)	22, 709, 1534, 1549, 1682, 1833
Hin6I	GCGC	2 cut(s)	2083, 2218
HinP1I	GCGC	2 cut(s)	2083, 2218
HincII	GTYRAC	2 cut(s)	757, 1258
HindII	GTYRAC	2 cut(s)	757, 1258
HindIII	AAGCTT	2 cut(s)	677, 2172
HinfI	GANTC	8 cut(s)	82, 268, 442, 595, 691, 1036, 1910, 1925
HpaI	GTTAAC	1 cut(s)	1258
HpaII	CCGG	4 cut(s)	350, 642, 656, 2304
HphI	GGTGA	4 cut(s)	983, 1325, 1657, 2348
Hpy166II	GTNNAC	9 cut(s)	101, 757, 1205, 1258, 1537, 1702, 2339, 2357, 2396
Hpy188I	TCNGA	8 cut(s)	134, 178, 465, 690, 1041, 1798, 2168, 2230
Hpy8I	GTNNAC	9 cut(s)	101, 757, 1205, 1258, 1537, 1702, 2339, 2357, 2396
Hpy99I	CGWCG	1 cut(s)	2048
HpyAV	CCTTC	7 cut(s)	190, 807, 962, 1736, 1852, 1915, 2264
HpyCH4III	ACNGT	5 cut(s)	282, 1505, 1611, 1706, 1791
HpyCH4IV	ACGT	3 cut(s)	237, 1417, 2353
HpyCH4V	TGCA	8 cut(s)	210, 1001, 1088, 1280, 1643, 1675, 1702, 2159
HpyF10VI	GCNNNNNNNGC	8 cut(s)	849, 872, 1649, 1783, 1832, 2048, 2080, 2220
HpyF3I	CTNAG	7 cut(s)	95, 131, 767, 1721, 2165, 2229, 2254
HpySE526I	ACGT	3 cut(s)	237, 1417, 2353
Hsp92I	GRCGYC	1 cut(s)	2083
Hsp92II	CATG	6 cut(s)	22, 709, 1534, 1549, 1682, 1833
HspAI	GCGC	2 cut(s)	2083, 2218
KasI	GGCGCC	1 cut(s)	2082
KpnI	GGTACC	1 cut(s)	2262
Ksp22I	TGATCA	1 cut(s)	1125
KspAI	GTTAAC	1 cut(s)	1258
LmnI	GCTCC	4 cut(s)	15, 776, 1217, 2249
LweI	GCATC	7 cut(s)	283, 1010, 1145, 1267, 1684, 1835, 1902
MabI	ACCWGGT	1 cut(s)	979
MaeI	CTAG	8 cut(s)	258, 513, 1266, 1629, 1713, 1770, 2367, 2425
MaeII	ACGT	3 cut(s)	237, 1417, 2353
MaeIII	GTNAC	3 cut(s)	1313, 1876, 1975
MboII	GAAGA	8 cut(s)	264, 705, 708, 1045, 1117, 1354, 2153, 2169
MfeI	CAATTG	2 cut(s)	1015, 1916
MflI	RGATCY	4 cut(s)	572, 1357, 1386, 1990
MhlI	GDGCHC	3 cut(s)	66, 1704, 1738
MlsI	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
MluNI	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
Mly113I	GGCGCC	1 cut(s)	2083
MlyI	GAGTC	3 cut(s)	91, 277, 451
MmeI	TCCRAC	4 cut(s)	156, 907, 1431, 1911
Mox20I	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
Mph1103I	ATGCAT	2 cut(s)	1003, 1282
MroXI	GAANNNNTTC	1 cut(s)	2173
MscI	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
MslI	CAYNNNNRTG	1 cut(s)	1683
Msp20I	TGGCCA	5 cut(s)	302, 375, 1347, 2051, 2322
MspA1I	CMGCKG	1 cut(s)	1297
MspI	CCGG	4 cut(s)	350, 642, 656, 2304
MspR9I	CCNGG	9 cut(s)	351, 643, 657, 981, 1101, 1595, 2053, 2247, 2420
MunI	CAATTG	2 cut(s)	1015, 1916
Mva1269I	GAATGC	5 cut(s)	212, 1280, 2200, 2290, 2362
MvaI	CCWGG	6 cut(s)	981, 1101, 1595, 2053, 2247, 2420
MwoI	GCNNNNNNNGC	8 cut(s)	849, 872, 1649, 1783, 1832, 2048, 2080, 2220
NarI	GGCGCC	1 cut(s)	2083
NciI	CCSGG	3 cut(s)	351, 643, 657
NlaIII	CATG	6 cut(s)	22, 709, 1534, 1549, 1682, 1833
NmuCI	GTSAC	2 cut(s)	1313, 1975
NsiI	ATGCAT	2 cut(s)	1003, 1282
NspI	RCATGY	2 cut(s)	709, 1549
PaeR7I	CTCGAG	1 cut(s)	2181
PagI	TCATGA	1 cut(s)	1678
PciI	ACATGT	1 cut(s)	705
PctI	GAATGC	5 cut(s)	212, 1280, 2200, 2290, 2362
PdmI	GAANNNNTTC	1 cut(s)	2173
PfeI	GAWTC	5 cut(s)	595, 691, 1036, 1910, 1925
PflMI	CCANNNNNTGG	1 cut(s)	926
PfoI	TCCNGGA	1 cut(s)	2245
PinAI	ACCGGT	1 cut(s)	2303
PleI	GAGTC	3 cut(s)	90, 276, 450
PluTI	GGCGCC	1 cut(s)	2086
PpsI	GAGTC	3 cut(s)	90, 276, 450
Ppu21I	YACGTR	1 cut(s)	2354
PpuMI	RGGWCCY	2 cut(s)	1558, 2055
PscI	ACATGT	1 cut(s)	705
Psp1406I	AACGTT	1 cut(s)	237
Psp5II	RGGWCCY	2 cut(s)	1558, 2055
Psp6I	CCWGG	6 cut(s)	979, 1099, 1593, 2051, 2245, 2418
PspGI	CCWGG	6 cut(s)	979, 1099, 1593, 2051, 2245, 2418
PspPI	GGNCC	8 cut(s)	165, 178, 428, 541, 739, 908, 1558, 2055
PspPPI	RGGWCCY	2 cut(s)	1558, 2055
PstI	CTGCAG	1 cut(s)	1090
PstNI	CAGNNNCTG	3 cut(s)	94, 1297, 2058
PsuI	RGATCY	4 cut(s)	572, 1357, 1386, 1990
PvuII	CAGCTG	1 cut(s)	1297
RsaI	GTAC	7 cut(s)	1439, 1477, 1538, 1577, 2149, 2260, 2397
RsaNI	GTAC	7 cut(s)	1438, 1476, 1537, 1576, 2148, 2259, 2396
RseI	CAYNNNNRTG	1 cut(s)	1683
Sau96I	GGNCC	8 cut(s)	165, 178, 428, 541, 739, 908, 1558, 2055
ScaI	AGTACT	1 cut(s)	2149
SchI	GAGTC	3 cut(s)	91, 277, 451
ScrFI	CCNGG	9 cut(s)	351, 643, 657, 981, 1101, 1595, 2053, 2247, 2420
SduI	GDGCHC	3 cut(s)	66, 1704, 1738
SexAI	ACCWGGT	1 cut(s)	979
SfaNI	GCATC	7 cut(s)	283, 1010, 1145, 1267, 1684, 1835, 1902
SfcI	CTRYAG	2 cut(s)	1086, 1308
SfiI	GGCCNNNNNGGCC	1 cut(s)	2048
SfoI	GGCGCC	1 cut(s)	2084
Sfr274I	CTCGAG	1 cut(s)	2181
SinI	GGWCC	5 cut(s)	178, 428, 541, 1558, 2055
SlaI	CTCGAG	1 cut(s)	2181
SmiMI	CAYNNNNRTG	1 cut(s)	1683
SmlI	CTYRAG	4 cut(s)	1737, 2077, 2105, 2181
SmoI	CTYRAG	4 cut(s)	1737, 2077, 2105, 2181
SsiI	CCGC	8 cut(s)	47, 872, 1092, 1213, 1655, 1709, 1780, 2087
SspDI	GGCGCC	1 cut(s)	2082
SspI	AATATT	1 cut(s)	886
SspMI	CTAG	8 cut(s)	258, 513, 1266, 1629, 1713, 1770, 2367, 2425
StyD4I	CCNGG	9 cut(s)	349, 641, 655, 979, 1099, 1593, 2051, 2245, 2418
StyI	CCWWGG	1 cut(s)	750
TaaI	ACNGT	5 cut(s)	282, 1505, 1611, 1706, 1791
TaiI	ACGT	3 cut(s)	240, 1420, 2356
TaqI	TCGA	5 cut(s)	291, 854, 1421, 1820, 2182
TaqII	GACCGA	1 cut(s)	126
TatI	WGTACW	5 cut(s)	1475, 1536, 1575, 2147, 2395
TauI	GCSGC	3 cut(s)	875, 1094, 1712
TfiI	GAWTC	5 cut(s)	595, 691, 1036, 1910, 1925
TscAI	CASTG	7 cut(s)	99, 837, 874, 1508, 1711, 1903, 1912
TseFI	GTSAC	2 cut(s)	1313, 1975
Tsp45I	GTSAC	2 cut(s)	1313, 1975
TspDTI	ATGAA	4 cut(s)	1352, 1519, 1806, 2334
TspRI	CASTG	7 cut(s)	99, 837, 874, 1508, 1711, 1903, 1912
Van91I	CCANNNNNTGG	1 cut(s)	926
VneI	GTGCAC	1 cut(s)	1700
VpaK11BI	GGWCC	5 cut(s)	178, 428, 541, 1558, 2055
XapI	RAATTY	4 cut(s)	451, 895, 1027, 1567
XceI	RCATGY	2 cut(s)	709, 1549
XhoI	CTCGAG	1 cut(s)	2181
XmiI	GTMKAC	1 cut(s)	2356
XmnI	GAANNNNTTC	1 cut(s)	2173
XspI	CTAG	8 cut(s)	258, 513, 1266, 1629, 1713, 1770, 2367, 2425
ZrmI	AGTACT	1 cut(s)	2149
Zsp2I	ATGCAT	2 cut(s)	1003, 1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.