MD15G1027100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002531
Physical Location & Seq
Forward (+)
1605871 .. 1610128
4258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1027100.v1.1.491

Sequence Viewer

Length: 2718 bp
ATGAAGTTGTTCTCTGTAACTGAAACGGCAATGGGGGCGAGAGCCCAAGTCGGCGCCTTTGCAGTAACGCTAATCTTGTTCGTGATTCAGAGCGGTTTTGCGCAGCAGGTTTCTTTGAGCTCCGCCGTGGAACGGTCGGCGTTACTGGAGCTCCGCTCGTCGTTGGGTCTCAGAAGCAAAGACTGGCCCATAAAGGCCGACCCTTGTTCGTCCTGGACGGGAGTCACATGCAGCAAGAACGGCCGAGTCACTGGGGTCACCGTGTCCGGTTTGAGGCGAACCCGACTGGGCCGGAGAAACCCCCGGTTCGCCGTCGATTCACTGACTGATTTCACCCTCTTGTCTATTTTCAACGCCTCCGGGTTTGCGCTTCCCGGGTCCATACCCGACTGGTTCGGCCAAAGACTTGGCGCACTCCAGGTGCTCGACCTTAGGTCTGCATCAGTCACTGGTCCTGTTCCTCAGTCGCTCGGTGATTTGAAAAATCTTACCGCTTTGTATTTATCTGCCAATGGCATCACTGGGATTTTGCCTTCTGCATTGATAAATATAACACAGTTGGAAATTCTTGATCTTTCGAGGAATGCGATTACTGGGTCAATACCTTCTGGCTTTGCCAATCTTGGAAAGCTTGAAAGTCTTGACCTTTCTTCAAATTTTTTATCTGGGCCAGTTCCACCAGGTTTGGGAACCCTTTCATGGCTTAAATTCTTGGACTTGTCTGATAACAGTCTCACCGATTCGATTCCGGTTCAGTTTAGTAACCTTTCCCGGTTAGTTGAGCTTAATCTTACCACGAATTCGTTGTCCGGGTCATTGCCTGCGGAGTTGAGAGGGTTGAGGAGTTTGAGGAGGATGGAGATTGGAGATAATAACCTTGAAGGTCCAGTACCGCAGGGTTTGTTTTCGAGTCTTGTTCAGCTGGAAGTTCTGGTTCTGAGTCGGAATAAATTTGATGGTGCTCTTCCCGGTGCATTGTGGTCACTTCCCAGTTTGCGCTTTCTTGATGTATCCCACAACAATTTTACAGGAACTCTGCTGGGCCTCGGTTCAAATGCTAGTGTTAGTGGTGCTGTGTTCAACCTTTCTAGTAATCTGTTATATGGAAATCTGACCTTCCCTGTTGGGAAATCTGTCTCAGTCGATTTGTCTGATAATTTTTTCCAAGGCAAGGTAGTCGAAGATAGTCTAAGCAATGGTACTCTTACTGGAAATTGCCTACAGATAGTACCAAATCAAAGGAGTTTGCAGGATTGTAGACAGTTTTATGAAGAGAGGAGTTTAACTTTCGATGATTTTGGGGTCCTACAACCAGCTCAGCCACCTTTCCTAGAACCCAAATCAAAGAGTAAGAACCGATTAACATTTATTTTGGTTGGGATATTTGGTGGACTTGGCTTTGTTGTGATTTTGGTACTGGTTCTGGTAGTGCTCCTGAAAATGTGCAATAAAGGCACAAATCAACGAGGAAGTGCAAATGTTGGGCCTGTTCCAGGAGATGACCCTTCACTCCCCAAAGATCTCATCTACGCATCAAGTCGAGGAGATTCATTTACGTATGAGCAGATTCTCCAGTTCACTGGTGCTTTCAGTGAAACAAATCTTATAAAACATGGACACTCTGGAGACATATACCGGGGATCCTTAGCAAGCGGAACCCATGTTGTTATCAAAAGGGTAAATTTGCATTCCTTAACGAAAGAATCATACACGATAGAAATGGATTTATTAGACAAGGTTTCACATACGAGATTGGTGCCAATTTTGGGTCACTGTTTAGAGCATGAGAACGAGAAGCTTCTGGTTTACAAATACATGCCAAATGGAGACTTGGCTCGTTCCTTGCACAGGGTTACCAACTTAGGAGATGGTAATTTACAGTCCCTGGATTGGATTACAAGATTAAAAATTGCAATTGGAGCCGCTGAAATCCTAGCTTACCTACACCATGAGTGCAGCCCTCCGCTCGTTCACAGAGATGTTCAAGCAAGCAGTATACTTCTTGATGATAAATTTGAGGTCCGGCTTGGAAGCTTGAGTGAGGTCCGTGTGCAAGAAGGGGATGGTAACCAAAATGTGATCACGAGATTGTTGCGGAAACAACAAACATCGGAACAAGGCCCTTATGCCTCGTCATCAGCTGCTTGTGCATATGATGTATACTGTTTTGGGAAGGTATTGCTTGAGCTTGTGACGGGTAAGATTGGCATCAGCAAATCCAATGATTCCTCTACAAGAGAGTTGTTGGAGCACATGGTGCGCTACATCAGCATATATGACAAGGAACTGTTGAATAAGATTGTTGACCCGTCTCTGATACTAGACGAAGATCTATTGGAAGAAGTTTGGTCTGTGGCAATTGTGGCCAGGTCCTGCCTCAACCCCAAGCCGTCTAAGCGTCCCCTGATGAGATACATCCTTAAAGCATTGGAAAACCCTCTAAAGGTGGTGAGAGAAGAGAGTTCCAGCTCTGGAAAGCTGCAAACAGCTTCATCGAGAAAATCCTGGAGCGCTACTTTCTTTGGTAGCTGGCATCATAGTTCATCATCAGAGAGCGCCACTGTTCCTGGCCAGACGAGCAGAGAGAGTATCAGTGGCTCAAAACAGTCGGGGCTAGTAGGTGGAGGGGAGTTCTCTGCATCACACAAAAGGTTATCAAATGAAATTTTCCCTGAGCCAATTGAAATACAAGATCTCGAGAGACAAGACGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

906

Amino Acids

98.43

Weight (kDa)

6.8

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 44 - 78 6.6e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 111 - 217 6.8e-07 Leucine-rich repeat region
LRR_8 PF13855 162 - 219 1.5e-11 Leucine rich repeat
LRR_8 PF13855 235 - 293 1.7e-07 Leucine rich repeat
LRR_8 PF13855 297 - 342 1.5e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 535 - 809 8.7e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 535 - 808 1.6e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
Acc16I TGCGCA 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 97
AccB1I GGYRCC 2 cut(s) 53, 1756
AccBSI CCGCTC 3 cut(s) 93, 156, 1966
AccI GTMKAC 3 cut(s) 1258, 1996, 2160
AclWI GGATC 2 cut(s) 1635, 1648
AcoI YGGCCR 4 cut(s) 241, 397, 2364, 2569
AcsI RAATTY 8 cut(s) 564, 655, 707, 799, 950, 1681, 2012, 2664
AcyI GRCGYC 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 2257
AfaI GTAC 4 cut(s) 891, 1201, 1230, 1416
AfeI AGCGCT 1 cut(s) 2512
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 8 cut(s) 212, 417, 679, 1492, 1884, 2366, 2504, 2566
Alw21I GWGCWC 6 cut(s) 122, 153, 426, 964, 1434, 2253
Alw26I GTCTC 7 cut(s) 173, 737, 1141, 1620, 1821, 2316, 2695
AlwI GGATC 2 cut(s) 1635, 1648
AlwNI CAGNNNCTG 3 cut(s) 449, 1885, 2373
Ama87I CYCGRG 2 cut(s) 374, 2696
Aor51HI AGCGCT 1 cut(s) 2512
ApeKI GCWGC 5 cut(s) 103, 231, 1956, 2141, 2479
ApoI RAATTY 8 cut(s) 564, 655, 707, 799, 950, 1681, 2012, 2664
ArsI GACNNNNNNTTYG 2 cut(s) 791, 823
Asp700I GAANNNNTTC 2 cut(s) 8, 694
AspLEI GCGC 8 cut(s) 56, 103, 370, 413, 999, 2262, 2513, 2558
AsuC2I CCSGG 8 cut(s) 304, 361, 375, 376, 772, 811, 969, 1637
AsuHPI GGTGA 5 cut(s) 250, 325, 485, 727, 2461
AvaI CYCGRG 2 cut(s) 374, 2696
AvaII GGWCC 7 cut(s) 378, 452, 884, 1303, 2020, 2044, 2370
AxyI CCTNAGG 1 cut(s) 431
BalI TGGCCA 2 cut(s) 2366, 2571
BamHI GGATCC 1 cut(s) 1640
BanI GGYRCC 2 cut(s) 53, 1756
BanII GRGCYC 3 cut(s) 46, 122, 153
BarI GAAGNNNNNNTAC 2 cut(s) 873, 905
BauI CACGAG 1 cut(s) 2083
Bbv12I GWGCWC 6 cut(s) 122, 153, 426, 964, 1434, 2253
BbvI GCAGC 5 cut(s) 115, 243, 1968, 2128, 2466
BccI CCATC 4 cut(s) 850, 950, 1862, 2057
BceAI ACGGC 5 cut(s) 42, 110, 256, 296, 2374
BcgI CGANNNNNNTGC 6 cut(s) 1159, 1193, 1738, 1772, 2074, 2108
BciT130I CCWGG 8 cut(s) 214, 419, 681, 1494, 1886, 2368, 2506, 2568
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 2079
BcnI CCSGG 8 cut(s) 304, 361, 375, 376, 772, 811, 969, 1637
BcoDI GTCTC 7 cut(s) 173, 737, 1141, 1620, 1821, 2316, 2695
BfaI CTAG 6 cut(s) 1059, 1089, 1331, 1934, 2321, 2615
BfmI CTRYAG 1 cut(s) 1220
BfoI RGCGCY 3 cut(s) 57, 2514, 2559
BfuAI ACCTGC 1 cut(s) 97
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 3 cut(s) 1519, 2329, 2692
BisI GCNGC 6 cut(s) 104, 232, 1923, 1957, 2142, 2480
BlpI GCTNAGC 1 cut(s) 1317
BlsI GCNGC 6 cut(s) 105, 233, 1924, 1958, 2143, 2481
Bme18I GGWCC 7 cut(s) 378, 452, 884, 1303, 2020, 2044, 2370
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 2 cut(s) 374, 2696
BmiI GGNNCC 8 cut(s) 55, 379, 691, 1304, 1642, 1657, 1758, 1921
BmrI ACTGGG 5 cut(s) 261, 296, 531, 603, 984
BmsI GCATC 6 cut(s) 449, 525, 1541, 2217, 2542, 2648
BmuI ACTGGG 5 cut(s) 261, 296, 531, 603, 984
BoxI GACNNNNGTC 1 cut(s) 221
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 5 cut(s) 167, 401, 1556, 1644, 2527
Bpu10I CCTNAGC 2 cut(s) 1645, 2673
Bpu1102I GCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 2 cut(s) 2056, 2204
BpuMI CCSGG 8 cut(s) 304, 361, 375, 376, 772, 811, 969, 1637
BsaAI YACGTR 1 cut(s) 1557
BsaBI GATNNNNATC 1 cut(s) 2207
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 7 cut(s) 126, 302, 374, 1045, 1165, 1636, 1884
BsaWI WCCGGW 2 cut(s) 266, 748
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 3 cut(s) 36, 815, 1201
Bse8I GATNNNNATC 1 cut(s) 2207
BseBI CCWGG 8 cut(s) 214, 419, 681, 1494, 1886, 2368, 2506, 2568
BseDI CCNNGG 7 cut(s) 126, 302, 374, 1045, 1165, 1636, 1884
BseGI GGATG 3 cut(s) 861, 2068, 2415
BseJI GATNNNNATC 1 cut(s) 2207
BseMI GCAATG 3 cut(s) 36, 815, 1201
BseMII CTCAG 6 cut(s) 184, 476, 929, 1152, 1331, 2664
BseRI GAGGAG 4 cut(s) 856, 865, 1291, 1557
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 5 cut(s) 115, 243, 1968, 2128, 2466
BseYI CCCAGC 1 cut(s) 1039
BsgI GTGCAG 1 cut(s) 1975
Bsh1285I CGRYCG 2 cut(s) 137, 244
BshNI GGYRCC 2 cut(s) 53, 1756
BsiEI CGRYCG 2 cut(s) 137, 244
BsiHKAI GWGCWC 6 cut(s) 122, 153, 426, 964, 1434, 2253
BsiHKCI CYCGRG 2 cut(s) 374, 2696
BslFI GGGAC 2 cut(s) 1867, 2385
BsmAI GTCTC 7 cut(s) 173, 737, 1141, 1620, 1821, 2316, 2695
BsmBI CGTCTC 1 cut(s) 2316
BsmFI GGGAC 2 cut(s) 1867, 2385
BsmI GAATGC 2 cut(s) 589, 1687
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 374, 2696
Bsp1286I GDGCHC 7 cut(s) 46, 122, 153, 426, 964, 1434, 2253
Bsp143I GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
Bsp1720I GCTNAGC 1 cut(s) 1317
BspCNI CTCAG 6 cut(s) 183, 475, 930, 1151, 1330, 2665
BspLI GGNNCC 8 cut(s) 55, 379, 691, 1304, 1642, 1657, 1758, 1921
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 2 cut(s) 1635, 1648
BspQI GCTCTTC 1 cut(s) 969
BspT107I GGYRCC 2 cut(s) 53, 1756
BspTNI GGTCTC 1 cut(s) 173
BsrBI CCGCTC 3 cut(s) 93, 156, 1966
BsrDI GCAATG 3 cut(s) 36, 815, 1201
BssECI CCNNGG 7 cut(s) 126, 302, 374, 1045, 1165, 1636, 1884
BssMI GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
BssNAI GTATAC 2 cut(s) 1997, 2161
BssNI GRCGYC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 2083
BssT1I CCWWGG 1 cut(s) 1165
Bst1107I GTATAC 2 cut(s) 1997, 2161
Bst2BI CACGAG 1 cut(s) 2083
Bst2UI CCWGG 8 cut(s) 214, 419, 681, 1494, 1886, 2368, 2506, 2568
Bst6I CTCTTC 3 cut(s) 969, 1266, 2451
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 2257
BstBAI YACGTR 1 cut(s) 1557
BstC8I GCNNGC 4 cut(s) 822, 1651, 1990, 2531
BstDSI CCRYGG 1 cut(s) 126
BstEII GGTNACC 3 cut(s) 256, 1852, 2066
BstENI CCTNNNNNAGG 2 cut(s) 1491, 1846
BstF5I GGATG 3 cut(s) 861, 2068, 2415
BstH2I RGCGCY 3 cut(s) 57, 2514, 2559
BstHHI GCGC 8 cut(s) 56, 103, 370, 413, 999, 2262, 2513, 2558
BstKTI GATC 6 cut(s) 574, 1522, 1643, 2082, 2332, 2695
BstMAI GTCTC 7 cut(s) 173, 737, 1141, 1620, 1821, 2316, 2695
BstMBI GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
BstMCI CGRYCG 2 cut(s) 137, 244
BstNI CCWGG 8 cut(s) 214, 419, 681, 1494, 1886, 2368, 2506, 2568
BstNSI RCATGY 2 cut(s) 231, 1819
BstPAI GACNNNNGTC 1 cut(s) 221
BstPI GGTNACC 3 cut(s) 256, 1852, 2066
BstSFI CTRYAG 1 cut(s) 1220
BstSNI TACGTA 1 cut(s) 1557
BstV1I GCAGC 5 cut(s) 115, 243, 1968, 2128, 2466
BstX2I RGATCY 4 cut(s) 1519, 1640, 2329, 2692
BstXI CCANNNNNNTGG 2 cut(s) 407, 1580
BstYI RGATCY 4 cut(s) 1519, 1640, 2329, 2692
BstZ17I GTATAC 2 cut(s) 1997, 2161
BstZI CGGCCG 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 431
BsuI GTATCC 1 cut(s) 1021
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 3 cut(s) 861, 2068, 2415
BtsIMutI CAGTG 9 cut(s) 249, 320, 447, 519, 1578, 1597, 1771, 2559, 2599
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 4 cut(s) 822, 1651, 1990, 2531
CaiI CAGNNNCTG 3 cut(s) 449, 1885, 2373
CfoI GCGC 8 cut(s) 56, 103, 370, 413, 999, 2262, 2513, 2558
Cfr9I CCCGGG 1 cut(s) 374
CseI GACGC 1 cut(s) 2387
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 4 cut(s) 890, 1200, 1229, 1415
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviAII CATG 8 cut(s) 228, 699, 1613, 1661, 1784, 1816, 1949, 2254
CviQI GTAC 4 cut(s) 890, 1200, 1229, 1415
DinI GGCGCC 1 cut(s) 55
DpnI GATC 6 cut(s) 573, 1521, 1642, 2081, 2331, 2694
DpnII GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
DraIII CACNNNGTG 1 cut(s) 2257
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 4 cut(s) 241, 397, 2364, 2569
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 969, 1266, 2451
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 3 cut(s) 969, 1266, 2451
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 2 cut(s) 120, 151
EclXI CGGCCG 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 1557
Eco130I CCWWGG 1 cut(s) 1165
Eco24I GRGCYC 3 cut(s) 46, 122, 153
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 7 cut(s) 378, 452, 884, 1303, 2020, 2044, 2370
Eco47III AGCGCT 1 cut(s) 2512
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 120, 151
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 2 cut(s) 374, 2696
Eco91I GGTNACC 3 cut(s) 256, 1852, 2066
EcoICRI GAGCTC 2 cut(s) 120, 151
EcoNI CCTNNNNNAGG 2 cut(s) 1491, 1846
EcoO109I RGGNCCY 3 cut(s) 1303, 2120, 2370
EcoO65I GGTNACC 3 cut(s) 256, 1852, 2066
EcoRI GAATTC 1 cut(s) 799
EcoRII CCWGG 8 cut(s) 212, 417, 679, 1492, 1884, 2366, 2504, 2566
EcoT14I CCWWGG 1 cut(s) 1165
EcoT38I GRGCYC 3 cut(s) 46, 122, 153
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 1165
Esp3I CGTCTC 1 cut(s) 2316
FaeI CATG 8 cut(s) 231, 702, 1616, 1664, 1787, 1819, 1952, 2257
FaqI GGGAC 2 cut(s) 1867, 2385
FatI CATG 8 cut(s) 227, 698, 1612, 1660, 1783, 1815, 1948, 2253
FauNDI CATATG 1 cut(s) 2152
FbaI TGATCA 1 cut(s) 2079
FblI GTMKAC 3 cut(s) 1258, 1996, 2160
Fnu4HI GCNGC 6 cut(s) 104, 232, 1923, 1957, 2142, 2480
FokI GGATG 3 cut(s) 868, 2075, 2402
FriOI GRGCYC 3 cut(s) 46, 122, 153
Fsp4HI GCNGC 6 cut(s) 104, 232, 1923, 1957, 2142, 2480
FspBI CTAG 6 cut(s) 1059, 1089, 1331, 1934, 2321, 2615
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 8 cut(s) 55, 102, 369, 412, 998, 2261, 2512, 2557
GluI GCNGC 6 cut(s) 104, 232, 1923, 1957, 2142, 2480
GsaI CCCAGC 1 cut(s) 1043
GsuI CTGGAG 5 cut(s) 167, 401, 1556, 1644, 2527
HaeII RGCGCY 3 cut(s) 57, 2514, 2559
HgaI GACGC 1 cut(s) 2387
HhaI GCGC 8 cut(s) 56, 103, 370, 413, 999, 2262, 2513, 2558
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 8 cut(s) 231, 702, 1616, 1664, 1787, 1819, 1952, 2257
Hin6I GCGC 8 cut(s) 54, 101, 368, 411, 997, 2260, 2511, 2556
HinP1I GCGC 8 cut(s) 54, 101, 368, 411, 997, 2260, 2511, 2556
HincII GTYRAC 1 cut(s) 2305
HindII GTYRAC 1 cut(s) 2305
HindIII AAGCTT 3 cut(s) 629, 1796, 2032
HphI GGTGA 5 cut(s) 250, 325, 485, 727, 2461
Hpy166II GTNNAC 8 cut(s) 1259, 1391, 1578, 1807, 1972, 1997, 2161, 2305
Hpy8I GTNNAC 8 cut(s) 1259, 1391, 1578, 1807, 1972, 1997, 2161, 2305
Hpy99I CGWCG 2 cut(s) 163, 317
HpyAV CCTTC 7 cut(s) 543, 615, 875, 1126, 1515, 2051, 2167
HpyCH4IV ACGT 1 cut(s) 1556
HpySE526I ACGT 1 cut(s) 1556
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 8 cut(s) 231, 702, 1616, 1664, 1787, 1819, 1952, 2257
HspAI GCGC 8 cut(s) 54, 101, 368, 411, 997, 2260, 2511, 2556
KasI GGCGCC 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 2079
Kzo9I GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 7 cut(s) 125, 148, 156, 1437, 1919, 2248, 2508
Lsp1109I GCAGC 5 cut(s) 115, 243, 1968, 2128, 2466
LweI GCATC 6 cut(s) 449, 525, 1541, 2217, 2542, 2648
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 6 cut(s) 1059, 1089, 1331, 1934, 2321, 2615
MaeII ACGT 1 cut(s) 1556
MalI GATC 6 cut(s) 573, 1521, 1642, 2081, 2331, 2694
MbiI CCGCTC 3 cut(s) 93, 156, 1966
MboI GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
MboII GAAGA 7 cut(s) 642, 956, 1193, 1283, 2339, 2351, 2468
MfeI CAATTG 3 cut(s) 1914, 2358, 2679
MflI RGATCY 4 cut(s) 1519, 1640, 2329, 2692
MhlI GDGCHC 7 cut(s) 46, 122, 153, 426, 964, 1434, 2253
MlsI TGGCCA 2 cut(s) 2366, 2571
MluNI TGGCCA 2 cut(s) 2366, 2571
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 4 cut(s) 231, 255, 919, 949
MmeI TCCRAC 3 cut(s) 540, 923, 2226
Mox20I TGGCCA 2 cut(s) 2366, 2571
MroXI GAANNNNTTC 2 cut(s) 8, 694
MscI TGGCCA 2 cut(s) 2366, 2571
MseI TTAA 7 cut(s) 705, 786, 1283, 1361, 1694, 1904, 2421
MslI CAYNNNNRTG 1 cut(s) 1977
Msp20I TGGCCA 2 cut(s) 2366, 2571
MspA1I CMGCKG 3 cut(s) 922, 1925, 2141
MunI CAATTG 3 cut(s) 1914, 2358, 2679
Mva1269I GAATGC 2 cut(s) 589, 1687
MvaI CCWGG 8 cut(s) 214, 419, 681, 1494, 1886, 2368, 2506, 2568
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 8 cut(s) 304, 361, 375, 376, 772, 811, 969, 1637
NdeI CATATG 1 cut(s) 2152
NdeII GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
NlaIII CATG 8 cut(s) 231, 702, 1616, 1664, 1787, 1819, 1952, 2257
NlaIV GGNNCC 8 cut(s) 55, 379, 691, 1304, 1642, 1657, 1758, 1921
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 7 cut(s) 223, 247, 256, 445, 981, 1769, 2191
NsbI TGCGCA 1 cut(s) 102
NspI RCATGY 2 cut(s) 231, 1819
PaeR7I CTCGAG 1 cut(s) 2696
PciSI GCTCTTC 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PctI GAATGC 2 cut(s) 589, 1687
PdmI GAANNNNTTC 2 cut(s) 8, 694
PfeI GAWTC 8 cut(s) 85, 317, 740, 745, 1547, 1567, 1703, 2225
PfoI TCCNGGA 3 cut(s) 212, 1492, 2504
PkrI GCNGC 6 cut(s) 105, 233, 1924, 1958, 2143, 2481
PleI GAGTC 4 cut(s) 230, 254, 918, 948
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 4 cut(s) 230, 254, 918, 948
Ppu21I YACGTR 1 cut(s) 1557
PpuMI RGGWCCY 2 cut(s) 1303, 2370
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 1607
Psp124BI GAGCTC 2 cut(s) 122, 153
Psp5II RGGWCCY 2 cut(s) 1303, 2370
Psp6I CCWGG 8 cut(s) 212, 417, 679, 1492, 1884, 2366, 2504, 2566
PspEI GGTNACC 3 cut(s) 256, 1852, 2066
PspFI CCCAGC 1 cut(s) 1039
PspGI CCWGG 8 cut(s) 212, 417, 679, 1492, 1884, 2366, 2504, 2566
PspN4I GGNNCC 8 cut(s) 55, 379, 691, 1304, 1642, 1657, 1758, 1921
PspPPI RGGWCCY 2 cut(s) 1303, 2370
PstNI CAGNNNCTG 3 cut(s) 449, 1885, 2373
PsuI RGATCY 4 cut(s) 1519, 1640, 2329, 2692
PvuII CAGCTG 2 cut(s) 922, 2141
RsaI GTAC 4 cut(s) 891, 1201, 1230, 1416
RsaNI GTAC 4 cut(s) 890, 1200, 1229, 1415
RseI CAYNNNNRTG 1 cut(s) 1977
SacI GAGCTC 2 cut(s) 122, 153
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 7 cut(s) 705, 786, 1283, 1361, 1694, 1904, 2421
SatI GCNGC 6 cut(s) 104, 232, 1923, 1957, 2142, 2480
Sau3AI GATC 6 cut(s) 571, 1519, 1640, 2079, 2329, 2692
SchI GAGTC 4 cut(s) 231, 255, 919, 949
SduI GDGCHC 7 cut(s) 46, 122, 153, 426, 964, 1434, 2253
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 6 cut(s) 449, 525, 1541, 2217, 2542, 2648
SfcI CTRYAG 1 cut(s) 1220
SfoI GGCGCC 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 2696
SinI GGWCC 7 cut(s) 378, 452, 884, 1303, 2020, 2044, 2370
SlaI CTCGAG 1 cut(s) 2696
SmaI CCCGGG 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 1977
SmlI CTYRAG 3 cut(s) 2035, 2183, 2696
SmoI CTYRAG 3 cut(s) 2035, 2183, 2696
SnaBI TACGTA 1 cut(s) 1557
SspDI GGCGCC 1 cut(s) 53
SspMI CTAG 6 cut(s) 1059, 1089, 1331, 1934, 2321, 2615
SstI GAGCTC 2 cut(s) 122, 153
StyI CCWWGG 1 cut(s) 1165
TaiI ACGT 1 cut(s) 1559
TauI GCSGC 1 cut(s) 1925
TfiI GAWTC 8 cut(s) 85, 317, 740, 745, 1547, 1567, 1703, 2225
Tru1I TTAA 7 cut(s) 705, 786, 1283, 1361, 1694, 1904, 2421
Tru9I TTAA 7 cut(s) 705, 786, 1283, 1361, 1694, 1904, 2421
TscAI CASTG 9 cut(s) 256, 327, 454, 526, 1585, 1597, 1778, 2566, 2599
TseFI GTSAC 7 cut(s) 223, 247, 256, 445, 981, 1769, 2191
TseI GCWGC 5 cut(s) 103, 231, 1956, 2141, 2479
Tsp45I GTSAC 7 cut(s) 223, 247, 256, 445, 981, 1769, 2191
TspDTI ATGAA 7 cut(s) 17, 687, 1284, 1539, 2481, 2532, 2676
TspGWI ACGGA 1 cut(s) 2036
TspMI CCCGGG 1 cut(s) 374
TspRI CASTG 9 cut(s) 256, 327, 454, 526, 1585, 1597, 1778, 2566, 2599
VpaK11BI GGWCC 7 cut(s) 378, 452, 884, 1303, 2020, 2044, 2370
XagI CCTNNNNNAGG 2 cut(s) 1491, 1846
XapI RAATTY 8 cut(s) 564, 655, 707, 799, 950, 1681, 2012, 2664
XceI RCATGY 2 cut(s) 231, 1819
XhoI CTCGAG 1 cut(s) 2696
XmaI CCCGGG 1 cut(s) 374
XmiI GTMKAC 3 cut(s) 1258, 1996, 2160
XmnI GAANNNNTTC 2 cut(s) 8, 694
XspI CTAG 6 cut(s) 1059, 1089, 1331, 1934, 2321, 2615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.