Rroxscaffold_7G00167160

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002531
Physical Location & Seq
Forward (+)
8406475 .. 8410816
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167160.1

Sequence Viewer

Length: 2700 bp
ATGATGAAGATGTACAGTCCGTTATCACATACGAAAACCCAAGTTGGCGTCTTTACAATAGCACTAGCTTTGTTATCCATTAACTGCGTTTTAGCCCAGAAAGGTTCTTTAAGCTCCGCCATAGAACGGTCGGCGTTACTGAACCTCCGGTCCTCTTTAGGAATCAGAAGCCGATACTGGCCCAGAAAGGCAGACCCTTGTTCTCACTGGACCGGAGTTGAATGCCGGAGCGGCCGAGTCATTGGAATCACAGTCTCCGGTTTGAGACGAACCACCGTCGGGCGGCGCAACCCCCAGTTCGCCGTGGATTCTTTAGCCAATTTTACATTCTTGGCTACTTTTAATGCTTCTGGGTTTTCGCTTCCGGGGTCTATACCCGACTGGTTTGGCCAGAGACTTGGTTCACTCCAAGTGCTTGATCTAAGGTCTGCTTCTGTCATTGGTGCTATACCTGAGTCACTTAGTAATTTGACAAAGCTTAAGTCTTTGCATTTATCTGGTAATGATATTACTGGTATTGTGCCTTCTGCATTGGAGAACTTGGGTGGCTTGGAGGTTCTTGATGTTTCGAGGAATTCATTTACTGGGTCCATACCTTCTGCTTTTGCCTCTCTTGGAAATCTTACAATGCTTGACCTTTCTTCAAATTTCCTATCCGGGCCAATTCCACCGGGTTTAGGCAACCTTTCAAGACTTGAGTACCTGAACCTGTCTGATAATAGTCTCACTGATTCTATACCGGTTCAGCTTGGCAAGCTTTCCCAGTTGCTCGGACTTGACTTTAGTAAGAACTCTTTGTCTGGGACATTGCCTGTGGAGTTGAGAGGGTTGAGGAGAGTGACGAAAATCGAGATTGAACATAATGATCTGGAAGGTTCACTGCCAGAGGGTTTGATTTCGAGTCTTGCTGAGCTGGAAGTTCTAGTTCTGAGTAGGAATAGACTTGGGGGTGCTCTTCCTAGTGCATTGTGGTCACTTCCCAGTTTGCAGTTCGTTGATTTATCCAGCAACAATTTTACAGGACCTCTGCCAAGCGTCGGTACAAATGGTAATTTTAGCAGAGCTGTGTTCAACCTCTCTGATAATGTCCTGTATGGAAATCTGACTTATCCTCTTGTGAAATTTGGCTCCATCGACTTGTCTGGGAACTTTTTTCAAGGCAAGGTATTGACAGATAATCAAAGAAATGCTACTCTTACTACAAATTGCCTCCAGTTGGTACCAAAACAGAGGAGTTTACAGGAATGTAGACAGTTTTATGAAGAAAGGAGTTTAGCTTTTGATGATTTTGGGGTACCTGGACCAGCCCAGCCCCCGTTTCTAGAACGCGACTCGAAGAGCAAAAATCGATTAATATATATACTGGTTGGAATATTTGGTGGACTAGGCTTCATTCTGATTTTGGTAGTGGTTCTGGCAGTGCTCCTGAAAAAGGGTAATAAAGCCACAAGTCAACAAGGAAGTGCAAATGTAGGGCCTGTTCCAGATGGAGACGGCCTTTCACTCCCTAAAGATCTCATGTATGCAACTGGCCTAGGAGATCCATTTACTTATGGGCAGATTCGCCAGATTGCTGGTGATTTTGGTGAAGAAAGTCTTATCAAACATGGACACTCTGGGGACATATTTCACGGTTCCTTGGTAAGTGGAACCCCTGTAGTTATCAAAAGGGTAGATCTGCATTCCTTCACGAAAGAATCATATATGATAGAACTGGATTTATTTAGCAAACTTTCACACACAAGATTGATCCCCCTTCTGGGGCATTGCTTGGAGCATGAGAGTGAGAAGCTTCTGGTTTACAAATATATGCCAAATGGAGACTTGGCTAGTTCCTTGCATAGAGTTGCAAATTCAGCCGATGGCAAATTACAGTCTTTGGATTGGATTACAAGACTAAAAATTGCAATTGGAGCTGCTGAAGTCCTAGCTTACTTACATCATGAGTGCAGCCCACCCCTTGTTCACAGAGATGTTCAAGCAAGCAGTATACTTCTGGATGACAAATTTGAGGTACGACTTGGAAGCTTGAGTGAGGTCCGTGCGCAAGAAGGGGATGCTAACCAAAATGTAATCACAAGATTGTTGCGGAAACAGCAAAGCTCTGAACAAAGCCCTTCTGCCCCGACGTCATTGGTGACTTGTGCTGACGATGTGTACTGTTTCGGTAAGGTTTTGCTTGAGCTTGTGACAGGCAAGCTCGGTATCAGCAAATCAGATGATGCCTCTACAAGAGAGTGGCTGGATCACACAGTGCGTCACATCAGCATATTTGAGATGGAACTGGTGAGTAAGATAGTTGACCCATCTCTTATAGTAGATGAGGATCTATTGGAAGAGGTGTGGGCCATGGCAATCGTGGCCAGGTCCTGCCTTAACCCCAAGCCTTCCAAACGTCCGCCCATGAAATACATCCTTAAAGCATTGGAAAACCCTCTAAAGGTGGTGAGGGAAGAGAGTTCAAGCTCCGCAAGGCTGCGGACAACTTCATCTAGAAGATCATGGAGCACTGCTTTCTTTGGAAGCTGGCGGCACAGCTCATCAGAGAATGCCACTGTTCCTGGCCATACAAACAGAGAAAGTATCAGTGGCTTAAAACAGTCGGGGCGAGCAGGTTCTCACGGCAGTGGTGGAAATGAGTTCTCTTCACGTAAAAGGTTGTCCAATGAAATTTTCCCTGAGCCAATCGAAATATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

899

Amino Acids

97.82

Weight (kDa)

8.17

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 132 - 211 1.5e-07 Leucine-rich repeat region
LRR_8 PF13855 159 - 216 5.8e-13 Leucine rich repeat
LRR_14 PF23598 225 - 311 3.5e-06 Leucine-rich repeat region
LRR_8 PF13855 231 - 290 3.2e-07 Leucine rich repeat
LRR_8 PF13855 293 - 339 1.2e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 534 - 754 6.4e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 534 - 807 2.4e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2132
Acc16I TGCGCA 1 cut(s) 2046
Acc36I ACCTGC 1 cut(s) 2603
Acc65I GGTACC 2 cut(s) 1219, 1294
AccB1I GGYRCC 2 cut(s) 1219, 1294
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 2 cut(s) 1249, 1990
AccII CGCG 1 cut(s) 1329
AciI CCGC 8 cut(s) 117, 231, 283, 2089, 2399, 2469, 2479, 2530
AclWI GGATC 4 cut(s) 1535, 1744, 2253, 2334
AcoI YGGCCR 4 cut(s) 232, 388, 2361, 2563
AcsI RAATTY 6 cut(s) 574, 646, 1121, 1852, 2006, 2670
AcuI CTGAAG 1 cut(s) 1941
AcyI GRCGYC 2 cut(s) 48, 2129
AdeI CACNNNGTG 1 cut(s) 2254
AfaI GTAC 7 cut(s) 14, 701, 1042, 1221, 1296, 2016, 2159
AflII CTTAAG 1 cut(s) 479
AgeI ACCGGT 1 cut(s) 739
AgsI TTSAA 8 cut(s) 221, 645, 690, 857, 1072, 1157, 1979, 2463
AjnI CCWGG 3 cut(s) 1297, 2363, 2560
AjuI GAANNNNNNNTTGG 4 cut(s) 311, 343, 2657, 2689
AleI CACNNNNGTG 1 cut(s) 2625
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
Alw21I GWGCWC 3 cut(s) 955, 1425, 2510
Alw26I GTCTC 6 cut(s) 259, 259, 388, 728, 1485, 1815
AlwI GGATC 4 cut(s) 1535, 1744, 2253, 2334
AlwNI CAGNNNCTG 1 cut(s) 2370
ApeKI GCWGC 3 cut(s) 1916, 1950, 2476
ApoI RAATTY 6 cut(s) 574, 646, 1121, 1852, 2006, 2670
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 739
Asp718I GGTACC 2 cut(s) 1219, 1294
AspA2I CCTAGG 1 cut(s) 1534
AspLEI GCGC 2 cut(s) 288, 2047
AsuC2I CCSGG 3 cut(s) 366, 658, 672
AsuHPI GGTGA 5 cut(s) 1589, 1598, 2149, 2299, 2458
AvaII GGWCC 7 cut(s) 150, 210, 588, 1022, 1301, 2038, 2367
AvrII CCTAGG 1 cut(s) 1534
BaeI ACNNNNGTAYC 2 cut(s) 683, 716
BalI TGGCCA 3 cut(s) 390, 2363, 2565
BanI GGYRCC 2 cut(s) 1219, 1294
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
Bbv12I GWGCWC 3 cut(s) 955, 1425, 2510
BbvI GCAGC 3 cut(s) 1903, 1962, 2463
BccI CCATC 5 cut(s) 1139, 1481, 1856, 2272, 2314
BceAI ACGGC 3 cut(s) 287, 1510, 2638
BciT130I CCWGG 3 cut(s) 1299, 2365, 2562
BcnI CCSGG 3 cut(s) 366, 658, 672
BcoDI GTCTC 6 cut(s) 259, 259, 388, 728, 1485, 1815
BfaI CTAG 9 cut(s) 65, 923, 960, 1322, 1385, 1535, 1830, 1928, 2493
BfmI CTRYAG 1 cut(s) 1656
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 2603
BglI GCCNNNNNGGC 1 cut(s) 231
BglII AGATCT 2 cut(s) 1513, 1675
BisI GCNGC 6 cut(s) 232, 284, 1917, 1951, 2477, 2531
BlnI CCTAGG 1 cut(s) 1534
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 6 cut(s) 233, 285, 1918, 1952, 2478, 2532
Bme1390I CCNGG 6 cut(s) 366, 658, 672, 1299, 2365, 2562
Bme18I GGWCC 7 cut(s) 150, 210, 588, 1022, 1301, 2038, 2367
BmiI GGNNCC 6 cut(s) 589, 1129, 1221, 1296, 1636, 1651
BmrFI CCNGG 6 cut(s) 366, 658, 672, 1299, 2365, 2562
BmrI ACTGGG 4 cut(s) 289, 594, 757, 975
BmsI GCATC 2 cut(s) 2047, 2212
BmuI ACTGGG 4 cut(s) 289, 594, 757, 975
BpmI CTGGAG 1 cut(s) 1196
Bpu10I CCTNAGC 1 cut(s) 2679
Bpu1102I GCTNAGC 1 cut(s) 909
BpuEI CTTGAG 3 cut(s) 716, 2050, 2201
BpuMI CCSGG 3 cut(s) 366, 658, 672
Bsa29I ATCGAT 1 cut(s) 1348
BsaAI YACGTR 1 cut(s) 2651
BsaBI GATNNNNATC 1 cut(s) 2325
BsaHI GRCGYC 2 cut(s) 48, 2129
BsaJI CCNNGG 5 cut(s) 303, 365, 1534, 1638, 2349
BsaWI WCCGGW 4 cut(s) 147, 212, 257, 739
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bse118I RCCGGY 1 cut(s) 739
Bse3DI GCAATG 2 cut(s) 806, 1765
Bse8I GATNNNNATC 1 cut(s) 2325
BseBI CCWGG 3 cut(s) 1299, 2365, 2562
BseCI ATCGAT 1 cut(s) 1348
BseDI CCNNGG 5 cut(s) 303, 365, 1534, 1638, 2349
BseGI GGATG 3 cut(s) 2005, 2062, 2412
BseJI GATNNNNATC 1 cut(s) 2325
BseMI GCAATG 2 cut(s) 806, 1765
BseMII CTCAG 4 cut(s) 444, 900, 920, 2670
BseRI GAGGAG 2 cut(s) 847, 1246
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 1903, 1962, 2463
BseYI CCCAGC 1 cut(s) 1308
BsgI GTGCAG 1 cut(s) 1969
Bsh1236I CGCG 1 cut(s) 1329
Bsh1285I CGRYCG 2 cut(s) 131, 235
BshNI GGYRCC 2 cut(s) 1219, 1294
BshTI ACCGGT 1 cut(s) 739
BshVI ATCGAT 1 cut(s) 1348
BsiEI CGRYCG 2 cut(s) 131, 235
BsiHKAI GWGCWC 3 cut(s) 955, 1425, 2510
BsiSI CCGG 8 cut(s) 148, 213, 226, 258, 365, 657, 671, 740
BslFI GGGAC 2 cut(s) 817, 1634
BsmAI GTCTC 6 cut(s) 259, 259, 388, 728, 1485, 1815
BsmBI CGTCTC 2 cut(s) 259, 1485
BsmFI GGGAC 2 cut(s) 817, 1634
BsmI GAATGC 3 cut(s) 227, 1681, 2554
Bsp1286I GDGCHC 3 cut(s) 955, 1425, 2510
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp19I CCATGG 1 cut(s) 2349
BspACI CCGC 8 cut(s) 117, 231, 283, 2089, 2399, 2469, 2479, 2530
BspCNI CTCAG 4 cut(s) 445, 901, 921, 2671
BspDI ATCGAT 1 cut(s) 1348
BspFNI CGCG 1 cut(s) 1329
BspHI TCATGA 1 cut(s) 1942
BspLI GGNNCC 6 cut(s) 589, 1129, 1221, 1296, 1636, 1651
BspMI ACCTGC 1 cut(s) 2603
BspPI GGATC 4 cut(s) 1535, 1744, 2253, 2334
BspQI GCTCTTC 2 cut(s) 960, 1331
BspT107I GGYRCC 2 cut(s) 1219, 1294
BspTI CTTAAG 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 2 cut(s) 806, 1765
BsrFI RCCGGY 1 cut(s) 739
BsrGI TGTACA 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 739
BssECI CCNNGG 5 cut(s) 303, 365, 1534, 1638, 2349
BssMI GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
BssNAI GTATAC 1 cut(s) 1991
BssNI GRCGYC 2 cut(s) 48, 2129
BssT1I CCWWGG 3 cut(s) 1534, 1638, 2349
Bst1107I GTATAC 1 cut(s) 1991
Bst2UI CCWGG 3 cut(s) 1299, 2365, 2562
Bst6I CTCTTC 5 cut(s) 960, 1331, 2331, 2448, 2650
BstACI GRCGYC 2 cut(s) 48, 2129
BstAFI CTTAAG 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 2651
BstC8I GCNNGC 5 cut(s) 755, 1984, 2198, 2528, 2610
BstDEI CTNAG 6 cut(s) 422, 453, 461, 909, 929, 2679
BstDSI CCRYGG 2 cut(s) 303, 2349
BstENI CCTNNNNNAGG 1 cut(s) 1430
BstF5I GGATG 3 cut(s) 2005, 2062, 2412
BstFNI CGCG 1 cut(s) 1329
BstHHI GCGC 2 cut(s) 288, 2047
BstKTI GATC 9 cut(s) 421, 868, 1516, 1543, 1678, 1752, 2248, 2329, 2501
BstMAI GTCTC 6 cut(s) 259, 259, 388, 728, 1485, 1815
BstMBI GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
BstMCI CGRYCG 2 cut(s) 131, 235
BstMWI GCNNNNNNNGC 6 cut(s) 231, 754, 1856, 1913, 2095, 2360
BstNI CCWGG 3 cut(s) 1299, 2365, 2562
BstSCI CCNGG 6 cut(s) 364, 656, 670, 1297, 2363, 2560
BstSFI CTRYAG 1 cut(s) 1656
BstUI CGCG 1 cut(s) 1329
BstV1I GCAGC 3 cut(s) 1903, 1962, 2463
BstX2I RGATCY 4 cut(s) 1513, 1540, 1675, 2326
BstXI CCANNNNNNTGG 2 cut(s) 398, 1574
BstYI RGATCY 4 cut(s) 1513, 1540, 1675, 2326
BstZ17I GTATAC 1 cut(s) 1991
BstZI CGGCCG 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 1348
BsuTUI ATCGAT 1 cut(s) 1348
BtgI CCRYGG 2 cut(s) 303, 2349
BtsCI GGATG 3 cut(s) 2005, 2062, 2412
BtsI GCAGTG 4 cut(s) 878, 1425, 2508, 2632
BtsIMutI CAGTG 9 cut(s) 205, 726, 878, 1425, 2259, 2508, 2553, 2593, 2632
BveI ACCTGC 1 cut(s) 2603
Cac8I GCNNGC 5 cut(s) 755, 1984, 2198, 2528, 2610
CaiI CAGNNNCTG 1 cut(s) 2370
CciI TCATGA 1 cut(s) 1942
CfoI GCGC 2 cut(s) 288, 2047
Cfr10I RCCGGY 1 cut(s) 739
ClaI ATCGAT 1 cut(s) 1348
CseI GACGC 3 cut(s) 37, 1024, 2246
Csp6I GTAC 7 cut(s) 13, 700, 1041, 1220, 1295, 2015, 2158
CspAI ACCGGT 1 cut(s) 739
CviAII CATG 7 cut(s) 1519, 1607, 1778, 1943, 2350, 2404, 2502
CviQI GTAC 7 cut(s) 13, 700, 1041, 1220, 1295, 2015, 2158
DdeI CTNAG 6 cut(s) 422, 453, 461, 909, 929, 2679
DpnI GATC 9 cut(s) 420, 867, 1515, 1542, 1677, 1751, 2247, 2328, 2500
DpnII GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
DraIII CACNNNGTG 1 cut(s) 2254
EaeI YGGCCR 4 cut(s) 232, 388, 2361, 2563
EagI CGGCCG 1 cut(s) 232
Eam1104I CTCTTC 5 cut(s) 960, 1331, 2331, 2448, 2650
EarI CTCTTC 5 cut(s) 960, 1331, 2331, 2448, 2650
EciI GGCGGA 2 cut(s) 106, 2388
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 1534, 1638, 2349
Eco47I GGWCC 7 cut(s) 150, 210, 588, 1022, 1301, 2038, 2367
Eco52I CGGCCG 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 1941
EcoNI CCTNNNNNAGG 1 cut(s) 1430
EcoO109I RGGNCCY 3 cut(s) 1022, 1475, 2367
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 1297, 2363, 2560
EcoT14I CCWWGG 3 cut(s) 1534, 1638, 2349
ErhI CCWWGG 3 cut(s) 1534, 1638, 2349
Esp3I CGTCTC 2 cut(s) 259, 1485
FaeI CATG 7 cut(s) 1522, 1610, 1781, 1946, 2353, 2407, 2505
FalI AAGNNNNNCTT 4 cut(s) 415, 447, 1581, 1613
FaqI GGGAC 2 cut(s) 817, 1634
FatI CATG 7 cut(s) 1518, 1606, 1777, 1942, 2349, 2403, 2501
FblI GTMKAC 2 cut(s) 1249, 1990
Fnu4HI GCNGC 6 cut(s) 232, 284, 1917, 1951, 2477, 2531
FokI GGATG 3 cut(s) 2012, 2069, 2399
Fsp4HI GCNGC 6 cut(s) 232, 284, 1917, 1951, 2477, 2531
FspBI CTAG 9 cut(s) 65, 923, 960, 1322, 1385, 1535, 1830, 1928, 2493
FspI TGCGCA 1 cut(s) 2046
GlaI GCGC 2 cut(s) 287, 2046
GluI GCNGC 6 cut(s) 232, 284, 1917, 1951, 2477, 2531
GsaI CCCAGC 1 cut(s) 1312
GsuI CTGGAG 1 cut(s) 1196
HapII CCGG 8 cut(s) 148, 213, 226, 258, 365, 657, 671, 740
HgaI GACGC 3 cut(s) 37, 1024, 2246
HhaI GCGC 2 cut(s) 288, 2047
Hin1I GRCGYC 2 cut(s) 48, 2129
Hin1II CATG 7 cut(s) 1522, 1610, 1781, 1946, 2353, 2407, 2505
Hin6I GCGC 2 cut(s) 286, 2045
HinP1I GCGC 2 cut(s) 286, 2045
HincII GTYRAC 2 cut(s) 1454, 2302
HindII GTYRAC 2 cut(s) 1454, 2302
HindIII AAGCTT 4 cut(s) 476, 755, 1790, 2026
HpaII CCGG 8 cut(s) 148, 213, 226, 258, 365, 657, 671, 740
HphI GGTGA 5 cut(s) 1589, 1598, 2149, 2299, 2458
Hpy99I CGWCG 3 cut(s) 281, 1040, 2131
HpyAV CCTTC 8 cut(s) 534, 606, 866, 1696, 1766, 2045, 2127, 2397
HpyCH4IV ACGT 3 cut(s) 2129, 2395, 2650
HpyF10VI GCNNNNNNNGC 6 cut(s) 231, 754, 1856, 1913, 2095, 2360
HpyF3I CTNAG 6 cut(s) 422, 453, 461, 909, 929, 2679
HpySE526I ACGT 3 cut(s) 2129, 2395, 2650
Hsp92I GRCGYC 2 cut(s) 48, 2129
Hsp92II CATG 7 cut(s) 1522, 1610, 1781, 1946, 2353, 2407, 2505
HspAI GCGC 2 cut(s) 286, 2045
KpnI GGTACC 2 cut(s) 1223, 1298
Kzo9I GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
LguI GCTCTTC 2 cut(s) 960, 1331
LmnI GCTCC 8 cut(s) 119, 228, 1133, 1428, 1774, 1913, 2471, 2505
Lsp1109I GCAGC 3 cut(s) 1903, 1962, 2463
LweI GCATC 2 cut(s) 2047, 2212
MaeI CTAG 9 cut(s) 65, 923, 960, 1322, 1385, 1535, 1830, 1928, 2493
MaeII ACGT 3 cut(s) 2129, 2395, 2650
MaeIII GTNAC 7 cut(s) 135, 456, 838, 972, 2137, 2188, 2258
MalI GATC 9 cut(s) 420, 867, 1515, 1542, 1677, 1751, 2247, 2328, 2500
MbiI CCGCTC 1 cut(s) 231
MboI GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
MfeI CAATTG 1 cut(s) 1908
MflI RGATCY 4 cut(s) 1513, 1540, 1675, 2326
MhlI GDGCHC 3 cut(s) 955, 1425, 2510
MlsI TGGCCA 3 cut(s) 390, 2363, 2565
MluNI TGGCCA 3 cut(s) 390, 2363, 2565
MlyI GAGTC 4 cut(s) 246, 464, 910, 1325
MmeI TCCRAC 1 cut(s) 1348
Mox20I TGGCCA 3 cut(s) 390, 2363, 2565
MscI TGGCCA 3 cut(s) 390, 2363, 2565
MseI TTAA 8 cut(s) 81, 110, 342, 480, 1352, 2376, 2418, 2594
MslI CAYNNNNRTG 3 cut(s) 1782, 1971, 2625
Msp20I TGGCCA 3 cut(s) 390, 2363, 2565
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 8 cut(s) 148, 213, 226, 258, 365, 657, 671, 740
MspR9I CCNGG 6 cut(s) 366, 658, 672, 1299, 2365, 2562
MunI CAATTG 1 cut(s) 1908
Mva1269I GAATGC 3 cut(s) 227, 1681, 2554
MvaI CCWGG 3 cut(s) 1299, 2365, 2562
MvnI CGCG 1 cut(s) 1329
MwoI GCNNNNNNNGC 6 cut(s) 231, 754, 1856, 1913, 2095, 2360
NciI CCSGG 3 cut(s) 366, 658, 672
NcoI CCATGG 1 cut(s) 2349
NdeII GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
NlaIII CATG 7 cut(s) 1522, 1610, 1781, 1946, 2353, 2407, 2505
NlaIV GGNNCC 6 cut(s) 589, 1129, 1221, 1296, 1636, 1651
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 6 cut(s) 456, 838, 972, 2137, 2188, 2258
NsbI TGCGCA 1 cut(s) 2046
OliI CACNNNNGTG 1 cut(s) 2625
PagI TCATGA 1 cut(s) 1942
PciSI GCTCTTC 2 cut(s) 960, 1331
PctI GAATGC 3 cut(s) 227, 1681, 2554
PfeI GAWTC 6 cut(s) 162, 246, 308, 731, 1561, 1697
PinAI ACCGGT 1 cut(s) 739
PkrI GCNGC 6 cut(s) 233, 285, 1918, 1952, 2478, 2532
PleI GAGTC 4 cut(s) 245, 463, 909, 1325
PpsI GAGTC 4 cut(s) 245, 463, 909, 1325
Ppu21I YACGTR 1 cut(s) 2651
PpuMI RGGWCCY 2 cut(s) 1022, 2367
PshBI ATTAAT 1 cut(s) 1352
Psp5II RGGWCCY 2 cut(s) 1022, 2367
Psp6I CCWGG 3 cut(s) 1297, 2363, 2560
PspFI CCCAGC 1 cut(s) 1308
PspGI CCWGG 3 cut(s) 1297, 2363, 2560
PspN4I GGNNCC 6 cut(s) 589, 1129, 1221, 1296, 1636, 1651
PspPPI RGGWCCY 2 cut(s) 1022, 2367
PsrI GAACNNNNNNTAC 2 cut(s) 1464, 1496
PstNI CAGNNNCTG 1 cut(s) 2370
PsuI RGATCY 4 cut(s) 1513, 1540, 1675, 2326
RsaI GTAC 7 cut(s) 14, 701, 1042, 1221, 1296, 2016, 2159
RsaNI GTAC 7 cut(s) 13, 700, 1041, 1220, 1295, 2015, 2158
RseI CAYNNNNRTG 3 cut(s) 1782, 1971, 2625
SapI GCTCTTC 2 cut(s) 960, 1331
SaqAI TTAA 8 cut(s) 81, 110, 342, 480, 1352, 2376, 2418, 2594
SatI GCNGC 6 cut(s) 232, 284, 1917, 1951, 2477, 2531
Sau3AI GATC 9 cut(s) 418, 865, 1513, 1540, 1675, 1749, 2245, 2326, 2498
SchI GAGTC 4 cut(s) 246, 464, 910, 1325
ScrFI CCNGG 6 cut(s) 366, 658, 672, 1299, 2365, 2562
SduI GDGCHC 3 cut(s) 955, 1425, 2510
SfaNI GCATC 2 cut(s) 2047, 2212
SfcI CTRYAG 1 cut(s) 1656
SinI GGWCC 7 cut(s) 150, 210, 588, 1022, 1301, 2038, 2367
SmiMI CAYNNNNRTG 3 cut(s) 1782, 1971, 2625
SmlI CTYRAG 4 cut(s) 479, 695, 2029, 2180
SmoI CTYRAG 4 cut(s) 479, 695, 2029, 2180
SsiI CCGC 8 cut(s) 117, 231, 283, 2089, 2399, 2469, 2479, 2530
SspI AATATT 1 cut(s) 1374
SspMI CTAG 9 cut(s) 65, 923, 960, 1322, 1385, 1535, 1830, 1928, 2493
StyD4I CCNGG 6 cut(s) 364, 656, 670, 1297, 2363, 2560
StyI CCWWGG 3 cut(s) 1534, 1638, 2349
TaiI ACGT 3 cut(s) 2132, 2398, 2653
TaqI TCGA 7 cut(s) 569, 849, 899, 1134, 1334, 1348, 2688
TatI WGTACW 2 cut(s) 12, 2157
TauI GCSGC 3 cut(s) 234, 286, 2533
TfiI GAWTC 6 cut(s) 162, 246, 308, 731, 1561, 1697
Tru1I TTAA 8 cut(s) 81, 110, 342, 480, 1352, 2376, 2418, 2594
Tru9I TTAA 8 cut(s) 81, 110, 342, 480, 1352, 2376, 2418, 2594
TscAI CASTG 9 cut(s) 212, 733, 885, 1425, 2259, 2515, 2560, 2593, 2632
TseFI GTSAC 6 cut(s) 456, 838, 972, 2137, 2188, 2258
TseI GCWGC 3 cut(s) 1916, 1950, 2476
Tsp45I GTSAC 6 cut(s) 456, 838, 972, 2137, 2188, 2258
TspDTI ATGAA 7 cut(s) 20, 567, 1275, 1381, 2420, 2478, 2682
TspGWI ACGGA 2 cut(s) 9, 2030
TspRI CASTG 9 cut(s) 212, 733, 885, 1425, 2259, 2515, 2560, 2593, 2632
Vha464I CTTAAG 1 cut(s) 479
VpaK11BI GGWCC 7 cut(s) 150, 210, 588, 1022, 1301, 2038, 2367
VspI ATTAAT 1 cut(s) 1352
XagI CCTNNNNNAGG 1 cut(s) 1430
XapI RAATTY 6 cut(s) 574, 646, 1121, 1852, 2006, 2670
XbaI TCTAGA 2 cut(s) 1321, 2492
XcmI CCANNNNNNNNNTGG 1 cut(s) 2356
XmaJI CCTAGG 1 cut(s) 1534
XmiI GTMKAC 2 cut(s) 1249, 1990
XspI CTAG 9 cut(s) 65, 923, 960, 1322, 1385, 1535, 1830, 1928, 2493
ZraI GACGTC 1 cut(s) 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.