FvH4_2g39760

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002531
Physical Location & Seq
Forward (+)
28463577 .. 28468130
4554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39760.t4

Sequence Viewer

Length: 2733 bp
ATGATGAAGATGTACAGTATGTTATCACATACTAAAACCCAAGTTGGAGTCTTTACAATAGCTATAGCTTTGTTCTTCTTCGTTAACATCGTTTTAGCCCAGCAAGATTACTTAAGCTCCGCCGTGGAACGGTCGGCGTTACTGGACCTCCGGTCGTCTTTAGGACTCAGAAGCAAAGACTGGCCCAGAAAGGCAGAGCCTTGTTCTAATTGGACCGGAGTTCAATGCCAGACCGGTCGAGTCATTGGAATCACTGTCTCCGGTTTGAGACGAACCACTCGTGGGCGGCGCAACCCCCAGTTCGCCGTTGATTCATTAGCCAATTTTACATCTTTGGTTTTGTTTAATGCTTCTGGGTTTTCGCTTCCGGGCTCTATACCTGACTGGTTTGGCCGGAGACTTAGTTCACTTGAGGTTCTTGATTTGAGGTCTGCTTCAGTCATTGGTGCTATACCTGAGTCTTTTGGTAATTTGAGAAAGCTTAGGTTTTTGTATTTATCTGGTAATGATATTACTGGTGCAGTGCCTTCTGCATTGGGGAACTTGGTTGAGTTGGAGGTTCTTGATGTTTCGAGGAATTCGTTTACTGGGGCAATACCTTCTGGTTTTGCCTCTCTTGGGAATCTTACAATGCTTAACCTTTCTTCGAATTTCCTATCCGGGCCAATTCCACCAGGTTTAGGTAATCTTTCGAGGCTTCAGTTCCTTAATCTGTCGGATAATAGTCTCGCTGGTTACATACCGGTTCAGCTTGGTGAGCTTTCACAGTTGCTGGAGCTTGACCTTAGCAAGAACTCTTTGTCTGGGGCATTACCTGTGGAGTTGAGAGGGTTGAGGAGTGTGAGGAAGATGGAGATTGAAGATAATGATCTGGCAGGTCCACTACCGGTGGGTTTGATTTTGAGTCTTGCTCAGCTGGAAGTTCTAGTTCTGAGTAGGAATAGACTTGAGGGTGCTCTTCCTAGTGCATTGTGGTCACTTCCCAGTTTGAGGTTTCTTGATTTATCCAGCAACAATTTTACAGGAGCTCTGCCAAGCATCAGTACAAATGGTAGTGTTAGAGGAGCTGTGTTCAACCTCTCTGATAATCTCCTATATGGAAATCTGACTTATCCGCTTGTGAAATATGGTTCCATCGACTTGTCTGGTAACTTTTTCCAAGGCAAGGTATTGGAAGATAGTCAAAGCAATGCTACCCTTACTACAAATTGCCTCCGGTTGGTACCAAGTCAGAGGAGTTTACCGGTATGTAGACAGTTTTATGAAGAAAGGCGTTTGGCTTTTGATAATTTTGGGGCACCTGAACCAGCCCAGCCACCGTTGCTAGAACCTGAATCGAAGAGCAGCAAAAATCGATTAATATATATACTGGCCGGGATATTTGGTGGACTAGGCTTCATTGTGATTTTGGTAGTGGTTCTAGTAGTGCTCCTGAAAAGGGGTAATAAAGCCACAAATCAACGAGGAAGTGCAAATGTAGGGCCTGTTCCAGATAAAGATGGCCTTTCACTCCCTAAAGATCTTGTGTATGCTTCAGGCCTAGGAGATCCATTTTCGTATGGGCAGATTCGCCAGATTGCTGGTGATTTTGGTGAAGAAAATCTGATCAAACATGGACACTCAGGGGACCTATTTCGCGGTTCCTTGGTAAGTGGAACCCCTGTAGTTATCAAAAGGGTAGATCTGAATTCCTTCACGAAAGATTCGTATATGATAGAAATGGATTTATTCAGCAAAGTTTCACACACAAGATTGATCCCACTTTTGGGGCATTGCTTGGAGCATGAGAGTGAGAAGCTTCTGGTTTACAAATATATGCCAAATGGAGACTTGGCTAGTTCCTTGCATAGAGTTACAAATTCAGCGGATGGAAAATTACAGTCTTTGGATTGGATTACTAGACTAAAAATTGCAATCGGAGCTGCTGAAGTCCTAGCTTACTTGCATCATGACTGCAGCCCACCTCTTGTTCACAGAGATGTTCAAGCGAGCAGTATACTTCTGGATGACAAATTTGAGGTACGACTTGGAAGTTTGAGTGAGGTCCGTGTGCAAGAAGGGGATGCAAATCAGAATGTAATCACAAGATTGTTGCGGAAACAACAAAGCTCTGAACAAAGCCCTTCTGCCGTGATGTCACCGGTGACTTGTGCTTATGATGTGTACTGTTTCGGTAAGGTGTTGCTTGAGCTTGTGACAGGCAAGCTTGGTATCAGCAAATCAGATGATGCCTCTACAAGAGAGTGGCTGGATCACACAGTACGTTACATCAGCATATTTGAGAAGGAACTGGTGAGTAAGATAGTCGACCCATCTTTGATAGTAGATGAGGATCTATTGGAAGAGGTATGGGCCATGGCAATCGTGGCCAGGTCTTGCCTTAACCCCAGACCTTCCAAGCGTCCGCCCATGAAATACATCCTTAAAGCATTGGAAAACCCTCTAAAGGTGGTGAGGGAAGAGAGTTTAAGCTCTGCAAGGCTGCGGACAACGTCATCTAGAAGATCATGGAGCACTGCTTTCTTTGGTAGCTGGCGGCACAGCTCATCAGAGAATGCTACTGTTCCTGGCCATACAAACAGAGATAGTATCAATGGCTTAAAACAGTCGGGGCGAGTAGGTTCTCACGGCAGTGTTGGAAATGAGTTCTCCTCTTCACGTAAAAGGTTGTCCAATGAAATTTTCCCCGAGCCAATTGAAATGCAAGATGTTGAGAGGCAAGAAGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

911

Amino Acids

99.81

Weight (kDa)

8.39

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 127 - 171 6.2e-07 Leucine rich repeat
LRR_14 PF23598 136 - 336 6.5e-17 Leucine-rich repeat region
LRR_8 PF13855 184 - 243 7.9e-09 Leucine rich repeat
LRR_8 PF13855 298 - 340 7.7e-08 Leucine rich repeat
LRR_4 PF12799 305 - 345 6.9e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 535 - 650 1.2e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 866
Acc65I GGTACC 1 cut(s) 1222
AccB1I GGYRCC 2 cut(s) 1222, 1297
AccI GTMKAC 3 cut(s) 1252, 1996, 2307
AccII CGCG 1 cut(s) 1638
AciI CCGC 9 cut(s) 120, 286, 1115, 1638, 1865, 2095, 2405, 2485, 2536
AclWI GGATC 4 cut(s) 1541, 1750, 2259, 2340
AcoI YGGCCR 4 cut(s) 391, 1371, 2367, 2569
AcsI RAATTY 6 cut(s) 577, 649, 1687, 1858, 2012, 2679
AcuI CTGAAG 4 cut(s) 420, 683, 1518, 1947
AfaI GTAC 6 cut(s) 14, 1045, 1224, 2022, 2165, 2262
AflII CTTAAG 1 cut(s) 112
AgeI ACCGGT 5 cut(s) 233, 742, 886, 1243, 2140
AgsI TTSAA 5 cut(s) 224, 860, 1075, 1985, 2699
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 3 cut(s) 673, 2369, 2566
AjuI GAANNNNNNNTTGG 2 cut(s) 2666, 2698
AleI CACNNNNGTG 1 cut(s) 2631
AloI GAACNNNNNNTCC 2 cut(s) 388, 420
Alw21I GWGCWC 4 cut(s) 958, 1030, 1431, 2516
Alw26I GTCTC 5 cut(s) 262, 262, 391, 731, 1821
AlwI GGATC 4 cut(s) 1541, 1750, 2259, 2340
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 2687
ApeKI GCWGC 4 cut(s) 1344, 1922, 1956, 2482
ApoI RAATTY 6 cut(s) 577, 649, 1687, 1858, 2012, 2679
AseI ATTAAT 1 cut(s) 1358
AsiGI ACCGGT 5 cut(s) 233, 742, 886, 1243, 2140
Asp700I GAANNNNTTC 1 cut(s) 1691
Asp718I GGTACC 1 cut(s) 1222
AspA2I CCTAGG 1 cut(s) 1540
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 9 cut(s) 145, 183, 213, 662, 878, 1481, 1627, 2044, 2352
AsuC2I CCSGG 3 cut(s) 369, 661, 1375
AsuHPI GGTGA 7 cut(s) 767, 1595, 1604, 2130, 2155, 2305, 2464
AsuII TTCGAA 1 cut(s) 647
AvaI CYCGRG 1 cut(s) 2687
AvaII GGWCC 5 cut(s) 145, 213, 878, 1627, 2044
AvrII CCTAGG 1 cut(s) 1540
BaeGI GKGCMC 1 cut(s) 1300
BalI TGGCCA 2 cut(s) 2369, 2571
BanI GGYRCC 2 cut(s) 1222, 1297
BanII GRGCYC 2 cut(s) 374, 1030
BauI CACGAG 1 cut(s) 279
Bbv12I GWGCWC 4 cut(s) 958, 1030, 1431, 2516
BbvI GCAGC 4 cut(s) 1356, 1909, 1968, 2469
BccI CCATC 5 cut(s) 844, 1142, 1493, 1862, 2320
BceAI ACGGC 4 cut(s) 107, 290, 2114, 2644
BciT130I CCWGG 3 cut(s) 675, 2371, 2568
BclI TGATCA 1 cut(s) 1605
BcnI CCSGG 3 cut(s) 369, 661, 1375
BcoDI GTCTC 5 cut(s) 262, 262, 391, 731, 1821
BfmI CTRYAG 3 cut(s) 63, 1662, 1954
BfrI CTTAAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 866
BglII AGATCT 2 cut(s) 1519, 1681
BisI GCNGC 6 cut(s) 287, 1345, 1923, 1957, 2483, 2537
BlnI CCTAGG 1 cut(s) 1540
BlpI GCTNAGC 1 cut(s) 912
BlsI GCNGC 6 cut(s) 288, 1346, 1924, 1958, 2484, 2538
Bme1390I CCNGG 6 cut(s) 369, 661, 675, 1375, 2371, 2568
Bme18I GGWCC 5 cut(s) 145, 213, 878, 1627, 2044
BmeRI GACNNNNNGTC 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 2687
BmgT120I GGNCC 9 cut(s) 145, 183, 213, 662, 878, 1481, 1627, 2044, 2352
BmiI GGNNCC 6 cut(s) 1132, 1224, 1299, 1628, 1642, 1657
BmrFI CCNGG 6 cut(s) 369, 661, 675, 1375, 2371, 2568
BmrI ACTGGG 3 cut(s) 292, 597, 978
BmsI GCATC 4 cut(s) 1047, 1954, 2053, 2218
BmuI ACTGGG 3 cut(s) 292, 597, 978
BplI GAGNNNNNCTC 4 cut(s) 895, 927, 2636, 2668
BpmI CTGGAG 1 cut(s) 794
Bpu10I CCTNAGC 2 cut(s) 482, 785
Bpu1102I GCTNAGC 1 cut(s) 912
Bpu14I TTCGAA 1 cut(s) 647
BpuEI CTTGAG 3 cut(s) 431, 968, 2207
BpuMI CCSGG 3 cut(s) 369, 661, 1375
Bsa29I ATCGAT 1 cut(s) 1354
BsaAI YACGTR 1 cut(s) 2660
BsaBI GATNNNNATC 3 cut(s) 867, 2067, 2331
BsaJI CCNNGG 5 cut(s) 123, 1159, 1540, 1644, 2355
BsaWI WCCGGW 9 cut(s) 150, 215, 233, 260, 742, 886, 1215, 1243, 2140
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 132, 162, 388, 418
Bse118I RCCGGY 5 cut(s) 233, 742, 886, 1243, 2140
Bse1I ACTGG 9 cut(s) 147, 185, 298, 389, 520, 592, 984, 1374, 2295
Bse3DI GCAATG 2 cut(s) 1195, 1771
Bse8I GATNNNNATC 3 cut(s) 867, 2067, 2331
BseBI CCWGG 3 cut(s) 675, 2371, 2568
BseCI ATCGAT 1 cut(s) 1354
BseDI CCNNGG 5 cut(s) 123, 1159, 1540, 1644, 2355
BseGI GGATG 4 cut(s) 1873, 2011, 2068, 2418
BseJI GATNNNNATC 3 cut(s) 867, 2067, 2331
BseMI GCAATG 2 cut(s) 1195, 1771
BseMII CTCAG 5 cut(s) 181, 447, 923, 926, 1635
BseNI ACTGG 9 cut(s) 147, 185, 298, 389, 520, 592, 984, 1374, 2295
BseRI GAGGAG 4 cut(s) 850, 1077, 1249, 2641
BseSI GKGCMC 1 cut(s) 1300
BseXI GCAGC 4 cut(s) 1356, 1909, 1968, 2469
BseYI CCCAGC 2 cut(s) 99, 1311
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 1638
Bsh1285I CGRYCG 3 cut(s) 134, 155, 238
BshNI GGYRCC 2 cut(s) 1222, 1297
BshTI ACCGGT 5 cut(s) 233, 742, 886, 1243, 2140
BshVI ATCGAT 1 cut(s) 1354
BsiEI CGRYCG 3 cut(s) 134, 155, 238
BsiHKAI GWGCWC 4 cut(s) 958, 1030, 1431, 2516
BsiHKCI CYCGRG 1 cut(s) 2687
BslFI GGGAC 1 cut(s) 1640
BsmAI GTCTC 5 cut(s) 262, 262, 391, 731, 1821
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 1 cut(s) 1640
BsmI GAATGC 1 cut(s) 2560
BsoBI CYCGRG 1 cut(s) 2687
Bsp119I TTCGAA 1 cut(s) 647
Bsp1286I GDGCHC 6 cut(s) 374, 958, 1030, 1300, 1431, 2516
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
Bsp1720I GCTNAGC 1 cut(s) 912
Bsp19I CCATGG 1 cut(s) 2355
BspACI CCGC 9 cut(s) 120, 286, 1115, 1638, 1865, 2095, 2405, 2485, 2536
BspCNI CTCAG 5 cut(s) 180, 448, 924, 925, 1634
BspDI ATCGAT 1 cut(s) 1354
BspFNI CGCG 1 cut(s) 1638
BspHI TCATGA 1 cut(s) 1948
BspLI GGNNCC 6 cut(s) 1132, 1224, 1299, 1628, 1642, 1657
BspMAI CTGCAG 1 cut(s) 1958
BspMI ACCTGC 1 cut(s) 866
BspPI GGATC 4 cut(s) 1541, 1750, 2259, 2340
BspQI GCTCTTC 2 cut(s) 963, 1334
BspT104I TTCGAA 1 cut(s) 647
BspT107I GGYRCC 2 cut(s) 1222, 1297
BspTI CTTAAG 1 cut(s) 112
BsrDI GCAATG 2 cut(s) 1195, 1771
BsrFI RCCGGY 5 cut(s) 233, 742, 886, 1243, 2140
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 9 cut(s) 147, 185, 298, 389, 520, 592, 984, 1374, 2295
BssAI RCCGGY 5 cut(s) 233, 742, 886, 1243, 2140
BssECI CCNNGG 5 cut(s) 123, 1159, 1540, 1644, 2355
BssMI GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
BssNAI GTATAC 1 cut(s) 1997
BssSI CACGAG 1 cut(s) 279
BssT1I CCWWGG 4 cut(s) 1159, 1540, 1644, 2355
Bst1107I GTATAC 1 cut(s) 1997
Bst2BI CACGAG 1 cut(s) 279
Bst2UI CCWGG 3 cut(s) 675, 2371, 2568
Bst6I CTCTTC 5 cut(s) 963, 1334, 2337, 2454, 2659
BstAFI CTTAAG 1 cut(s) 112
BstAUI TGTACA 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 2660
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 1990, 2204, 2534
BstDEI CTNAG 8 cut(s) 167, 401, 456, 482, 785, 912, 932, 1621
BstDSI CCRYGG 2 cut(s) 123, 2355
BstF5I GGATG 4 cut(s) 1873, 2011, 2068, 2418
BstFNI CGCG 1 cut(s) 1638
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 9 cut(s) 871, 1522, 1549, 1608, 1684, 1758, 2254, 2335, 2507
BstMAI GTCTC 5 cut(s) 262, 262, 391, 731, 1821
BstMBI GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
BstMCI CGRYCG 3 cut(s) 134, 155, 238
BstMWI GCNNNNNNNGC 4 cut(s) 757, 1321, 1919, 2366
BstNI CCWGG 3 cut(s) 675, 2371, 2568
BstSCI CCNGG 6 cut(s) 367, 659, 673, 1373, 2369, 2566
BstSFI CTRYAG 3 cut(s) 63, 1662, 1954
BstSLI GKGCMC 1 cut(s) 1300
BstUI CGCG 1 cut(s) 1638
BstV1I GCAGC 4 cut(s) 1356, 1909, 1968, 2469
BstX2I RGATCY 4 cut(s) 1519, 1546, 1681, 2332
BstXI CCANNNNNNTGG 1 cut(s) 1580
BstYI RGATCY 4 cut(s) 1519, 1546, 1681, 2332
BstZ17I GTATAC 1 cut(s) 1997
Bsu15I ATCGAT 1 cut(s) 1354
BsuTUI ATCGAT 1 cut(s) 1354
BtgI CCRYGG 2 cut(s) 123, 2355
BtsCI GGATG 4 cut(s) 1873, 2011, 2068, 2418
BtsI GCAGTG 3 cut(s) 528, 2514, 2638
BtsIMutI CAGTG 4 cut(s) 252, 528, 2514, 2638
BveI ACCTGC 1 cut(s) 866
Cac8I GCNNGC 3 cut(s) 1990, 2204, 2534
CaiI CAGNNNCTG 1 cut(s) 772
CciI TCATGA 1 cut(s) 1948
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 5 cut(s) 233, 742, 886, 1243, 2140
Cfr13I GGNCC 9 cut(s) 145, 183, 213, 662, 878, 1481, 1627, 2044, 2352
ClaI ATCGAT 1 cut(s) 1354
CseI GACGC 1 cut(s) 2390
CsiI ACCWGGT 1 cut(s) 673
Csp6I GTAC 6 cut(s) 13, 1044, 1223, 2021, 2164, 2261
CspAI ACCGGT 5 cut(s) 233, 742, 886, 1243, 2140
CviAII CATG 6 cut(s) 1613, 1784, 1949, 2356, 2410, 2508
CviQI GTAC 6 cut(s) 13, 1044, 1223, 2021, 2164, 2261
DdeI CTNAG 8 cut(s) 167, 401, 456, 482, 785, 912, 932, 1621
DpnI GATC 9 cut(s) 870, 1521, 1548, 1607, 1683, 1757, 2253, 2334, 2506
DpnII GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
DriI GACNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 4 cut(s) 391, 1371, 2367, 2569
Eam1104I CTCTTC 5 cut(s) 963, 1334, 2337, 2454, 2659
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 5 cut(s) 963, 1334, 2337, 2454, 2659
EciI GGCGGA 2 cut(s) 109, 2394
Ecl136II GAGCTC 1 cut(s) 1028
Eco130I CCWWGG 4 cut(s) 1159, 1540, 1644, 2355
Eco147I AGGCCT 1 cut(s) 1539
Eco24I GRGCYC 2 cut(s) 374, 1030
Eco47I GGWCC 5 cut(s) 145, 213, 878, 1627, 2044
Eco53kI GAGCTC 1 cut(s) 1028
Eco57I CTGAAG 4 cut(s) 420, 683, 1518, 1947
Eco88I CYCGRG 1 cut(s) 2687
EcoICRI GAGCTC 1 cut(s) 1028
EcoO109I RGGNCCY 2 cut(s) 1481, 1627
EcoRI GAATTC 2 cut(s) 577, 1687
EcoRII CCWGG 3 cut(s) 673, 2369, 2566
EcoT14I CCWWGG 4 cut(s) 1159, 1540, 1644, 2355
EcoT38I GRGCYC 2 cut(s) 374, 1030
ErhI CCWWGG 4 cut(s) 1159, 1540, 1644, 2355
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 6 cut(s) 1616, 1787, 1952, 2359, 2413, 2511
FalI AAGNNNNNCTT 2 cut(s) 1488, 1520
FaqI GGGAC 1 cut(s) 1640
FatI CATG 6 cut(s) 1612, 1783, 1948, 2355, 2409, 2507
FbaI TGATCA 1 cut(s) 1605
FblI GTMKAC 3 cut(s) 1252, 1996, 2307
Fnu4HI GCNGC 6 cut(s) 287, 1345, 1923, 1957, 2483, 2537
FokI GGATG 4 cut(s) 1880, 2018, 2075, 2405
FriOI GRGCYC 2 cut(s) 374, 1030
Fsp4HI GCNGC 6 cut(s) 287, 1345, 1923, 1957, 2483, 2537
GlaI GCGC 1 cut(s) 290
GluI GCNGC 6 cut(s) 287, 1345, 1923, 1957, 2483, 2537
GsaI CCCAGC 2 cut(s) 103, 1315
GsuI CTGGAG 1 cut(s) 794
HgaI GACGC 1 cut(s) 2390
HhaI GCGC 1 cut(s) 291
Hin1II CATG 6 cut(s) 1616, 1787, 1952, 2359, 2413, 2511
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 2 cut(s) 85, 2308
HindII GTYRAC 2 cut(s) 85, 2308
HindIII AAGCTT 3 cut(s) 479, 1796, 2204
HpaI GTTAAC 1 cut(s) 85
HphI GGTGA 7 cut(s) 767, 1595, 1604, 2130, 2155, 2305, 2464
Hpy188III TCNNGA 9 cut(s) 419, 563, 998, 1432, 1490, 1696, 1949, 2003, 2499
HpyAV CCTTC 7 cut(s) 537, 609, 1702, 2051, 2133, 2278, 2403
HpyCH4IV ACGT 3 cut(s) 2263, 2492, 2659
HpyF10VI GCNNNNNNNGC 4 cut(s) 757, 1321, 1919, 2366
HpyF3I CTNAG 8 cut(s) 167, 401, 456, 482, 785, 912, 932, 1621
HpySE526I ACGT 3 cut(s) 2263, 2492, 2659
Hsp92II CATG 6 cut(s) 1616, 1787, 1952, 2359, 2413, 2511
HspAI GCGC 1 cut(s) 289
KpnI GGTACC 1 cut(s) 1226
Ksp22I TGATCA 1 cut(s) 1605
KspAI GTTAAC 1 cut(s) 85
Kzo9I GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
LguI GCTCTTC 2 cut(s) 963, 1334
LmnI GCTCC 8 cut(s) 122, 775, 1025, 1064, 1434, 1780, 1919, 2511
Lsp1109I GCAGC 4 cut(s) 1356, 1909, 1968, 2469
LweI GCATC 4 cut(s) 1047, 1954, 2053, 2218
MabI ACCWGGT 1 cut(s) 673
MaeII ACGT 3 cut(s) 2263, 2492, 2659
MaeIII GTNAC 9 cut(s) 138, 734, 975, 1148, 1852, 2136, 2143, 2194, 2264
MalI GATC 9 cut(s) 870, 1521, 1548, 1607, 1683, 1757, 2253, 2334, 2506
MboI GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
MfeI CAATTG 1 cut(s) 2694
MflI RGATCY 4 cut(s) 1519, 1546, 1681, 2332
MhlI GDGCHC 6 cut(s) 374, 958, 1030, 1300, 1431, 2516
MlsI TGGCCA 2 cut(s) 2369, 2571
MluNI TGGCCA 2 cut(s) 2369, 2571
MlyI GAGTC 5 cut(s) 57, 159, 249, 467, 913
MmeI TCCRAC 4 cut(s) 25, 534, 696, 2617
Mox20I TGGCCA 2 cut(s) 2369, 2571
MroXI GAANNNNTTC 1 cut(s) 1691
MscI TGGCCA 2 cut(s) 2369, 2571
MslI CAYNNNNRTG 3 cut(s) 1788, 1977, 2631
Msp20I TGGCCA 2 cut(s) 2369, 2571
MspA1I CMGCKG 2 cut(s) 916, 1865
MspCI CTTAAG 1 cut(s) 112
MspR9I CCNGG 6 cut(s) 369, 661, 675, 1375, 2371, 2568
MunI CAATTG 1 cut(s) 2694
Mva1269I GAATGC 1 cut(s) 2560
MvaI CCWGG 3 cut(s) 675, 2371, 2568
MvnI CGCG 1 cut(s) 1638
MwoI GCNNNNNNNGC 4 cut(s) 757, 1321, 1919, 2366
NciI CCSGG 3 cut(s) 369, 661, 1375
NcoI CCATGG 1 cut(s) 2355
NdeII GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
NlaIII CATG 6 cut(s) 1616, 1787, 1952, 2359, 2413, 2511
NlaIV GGNNCC 6 cut(s) 1132, 1224, 1299, 1628, 1642, 1657
NmuCI GTSAC 4 cut(s) 975, 2136, 2143, 2194
NspV TTCGAA 1 cut(s) 647
OliI CACNNNNGTG 1 cut(s) 2631
PagI TCATGA 1 cut(s) 1948
PceI AGGCCT 1 cut(s) 1539
PciSI GCTCTTC 2 cut(s) 963, 1334
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 277, 578, 1703
PctI GAATGC 1 cut(s) 2560
PdmI GAANNNNTTC 1 cut(s) 1691
PfeI GAWTC 6 cut(s) 249, 311, 622, 1334, 1567, 1703
PflFI GACNNNGTC 1 cut(s) 2491
PinAI ACCGGT 5 cut(s) 233, 742, 886, 1243, 2140
PkrI GCNGC 6 cut(s) 288, 1346, 1924, 1958, 2484, 2538
PleI GAGTC 5 cut(s) 56, 159, 248, 466, 912
PpsI GAGTC 5 cut(s) 56, 159, 248, 466, 912
Ppu21I YACGTR 1 cut(s) 2660
PpuMI RGGWCCY 1 cut(s) 1627
PshBI ATTAAT 1 cut(s) 1358
Psp124BI GAGCTC 1 cut(s) 1030
Psp5II RGGWCCY 1 cut(s) 1627
Psp6I CCWGG 3 cut(s) 673, 2369, 2566
PspFI CCCAGC 2 cut(s) 99, 1311
PspGI CCWGG 3 cut(s) 673, 2369, 2566
PspN4I GGNNCC 6 cut(s) 1132, 1224, 1299, 1628, 1642, 1657
PspPI GGNCC 9 cut(s) 145, 183, 213, 662, 878, 1481, 1627, 2044, 2352
PspPPI RGGWCCY 1 cut(s) 1627
PsrI GAACNNNNNNTAC 2 cut(s) 1470, 1502
PstI CTGCAG 1 cut(s) 1958
PstNI CAGNNNCTG 1 cut(s) 772
PsuI RGATCY 4 cut(s) 1519, 1546, 1681, 2332
PsyI GACNNNGTC 1 cut(s) 2491
PvuII CAGCTG 1 cut(s) 916
RsaI GTAC 6 cut(s) 14, 1045, 1224, 2022, 2165, 2262
RsaNI GTAC 6 cut(s) 13, 1044, 1223, 2021, 2164, 2261
RseI CAYNNNNRTG 3 cut(s) 1788, 1977, 2631
SacI GAGCTC 1 cut(s) 1030
SalI GTCGAC 1 cut(s) 2306
SapI GCTCTTC 2 cut(s) 963, 1334
SatI GCNGC 6 cut(s) 287, 1345, 1923, 1957, 2483, 2537
Sau3AI GATC 9 cut(s) 868, 1519, 1546, 1605, 1681, 1755, 2251, 2332, 2504
Sau96I GGNCC 9 cut(s) 145, 183, 213, 662, 878, 1481, 1627, 2044, 2352
SchI GAGTC 5 cut(s) 57, 159, 249, 467, 913
ScrFI CCNGG 6 cut(s) 369, 661, 675, 1375, 2371, 2568
SduI GDGCHC 6 cut(s) 374, 958, 1030, 1300, 1431, 2516
SexAI ACCWGGT 1 cut(s) 673
SfaNI GCATC 4 cut(s) 1047, 1954, 2053, 2218
SfcI CTRYAG 3 cut(s) 63, 1662, 1954
SfuI TTCGAA 1 cut(s) 647
SgrAI CRCCGGYG 1 cut(s) 2140
SinI GGWCC 5 cut(s) 145, 213, 878, 1627, 2044
SmiMI CAYNNNNRTG 3 cut(s) 1788, 1977, 2631
SmlI CTYRAG 4 cut(s) 112, 410, 947, 2186
SmoI CTYRAG 4 cut(s) 112, 410, 947, 2186
SseBI AGGCCT 1 cut(s) 1539
SsiI CCGC 9 cut(s) 120, 286, 1115, 1638, 1865, 2095, 2405, 2485, 2536
SstI GAGCTC 1 cut(s) 1030
StuI AGGCCT 1 cut(s) 1539
StyD4I CCNGG 6 cut(s) 367, 659, 673, 1373, 2369, 2566
StyI CCWWGG 4 cut(s) 1159, 1540, 1644, 2355
TaiI ACGT 3 cut(s) 2266, 2495, 2662
TaqI TCGA 8 cut(s) 238, 572, 647, 692, 1137, 1337, 1354, 2307
TatI WGTACW 3 cut(s) 12, 1043, 2163
TauI GCSGC 2 cut(s) 289, 2539
TfiI GAWTC 6 cut(s) 249, 311, 622, 1334, 1567, 1703
TscAI CASTG 4 cut(s) 259, 528, 2521, 2638
TseFI GTSAC 4 cut(s) 975, 2136, 2143, 2194
TseI GCWGC 4 cut(s) 1344, 1922, 1956, 2482
Tsp45I GTSAC 4 cut(s) 975, 2136, 2143, 2194
TspDTI ATGAA 6 cut(s) 20, 303, 1278, 1387, 2426, 2691
TspGWI ACGGA 1 cut(s) 2036
TspRI CASTG 4 cut(s) 259, 528, 2521, 2638
Tth111I GACNNNGTC 1 cut(s) 2491
Vha464I CTTAAG 1 cut(s) 112
VpaK11BI GGWCC 5 cut(s) 145, 213, 878, 1627, 2044
VspI ATTAAT 1 cut(s) 1358
XapI RAATTY 6 cut(s) 577, 649, 1687, 1858, 2012, 2679
XbaI TCTAGA 1 cut(s) 2498
XcmI CCANNNNNNNNNTGG 1 cut(s) 2362
XmaJI CCTAGG 1 cut(s) 1540
XmiI GTMKAC 3 cut(s) 1252, 1996, 2307
XmnI GAANNNNTTC 1 cut(s) 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.