MD08G1031300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002531
Physical Location & Seq
Forward (+)
2242245 .. 2246357
4113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1031300.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGAAGTTGTTATCTGTAACTAAAACGGCAATGGGGGCGAGAGCCCAAGTCGGCGCCTTTGCCGTAGCGTTAATCTTGTTCGTGATTCAGAGTGGTTTTGCGCAGCAGGGTTCTTTGAGCTCCGACATGGAGCGGTCGGCGTTACTGGAGCTCCGGTCTTCGTTGGGTCTCAGAAGCAAAAACTGGCCCAGAAAGGCCGACCCATGTTCATCCTGGACCGGAGTCACATGCAGCAAGAACGGCCGTGTCACTGGAATTACCGTGTCCGGTTTGAGGCGAACCCGAGTGGGCCAGCAAAACCCCCGGTTCTCCGTCGATTCACTGGCTAATATCACCCTCTTGGCTTCTTTCAACGCCTCCGGGTTCGCGCTTCCTGGGTCCATACCCGACTGGTTTGGCCAAAGACTTGGTGCACTCCAGGTGCTCGACCTTAGGTCTGCGTCAGTCATTGGTTCTATTCCCCAGTCGCTTGGTAATTTGAAAAATCTTACTTCTTTGTATTTATCTGGCAATGATATTACTGGGATTTTACCTTCTGCGTTGATAAACATAACAGAGTTGGAAATTCTTGATCTTTCGAGGAATGCGATTACTGGGTCCATACCTTCTGGCTTTGCCAATCTTGGAAAGCTTGAAAGTCTTGACCTTTCTTCAAATTTCTTATCTGGGTCGGTTTCACCAGGTTTGGGAACCCTTTCAAGGCTTAAGTTCTTGGACTTGTCTGATAATAGTCTCACCGATTCGATTCCGGTTCAGTTTAGTAACCTTTCCCGGTTGGTTGAGCTTGATCTTAGCAAGAACACTTTGTCTGGGTCATTGCCTGTGGAACTGAGAGGGTTGAGGAGTTTGAGGAGGATGGGTATTGGAGATAATGCTCTGGAAGGTCCATTGCCGGAGGGTTTGTTTTCGAGTCTTGTTCAGCTGGAAGTTCTGGTTCTGAGTCGGAATAAATTTGATGGTGCTCTTCCTGGTGCCTTGTGGTCACTTCCCAGTTTGCGATTTCTCGATGTATCCCGCAACAATTTTACAGGAACTCTACCGAGCCTGGGTTCAAATGCTAGTGTTAGTGGCGCTGTGTTCAACCTTTCTCATAATCTGTTATATGGAAATCTTACATTTCCTGTTGGGAAATTTGTCTCAATCGATTTGTCTGATAATTTTTTCCAAGGCAAGGTATTAGAAGATAGTCAAAGCAATGATACTCTTACTGGAAATTGCCTACAGATTGTACCAAATCAAAGGAGTTTGCAGGATTGTAGGCAGTTTTATGAAGAGAGGGGTTTAAGTTTCGATGATTTTGGGGCCCTAGAACCAGCTCAGCCACCTTTCCTAGAACCTGAATCAAAGAGCAAGAACCGATTAACATTTATATTGGTTGGGATATTTGGTGGACTCGGCTTCATTGTGATTTTGGTACTGGTTCTGGTAGTGCTCCTGAAAATGTGCAATAAAGGCACAAATCAACGTGGAAGTGCAAATGTTGGGCCTGTTCCAGAAGGAGATGACCCTTCACTCCCCAAAGATCTCATCGATGCATCAGGTCGAGGAGATTCATTTACGTATGAGCAGATTCTCCATTCCACTTCTGCTTTCAGTGAAGCAAATCTTATAAAACATGGACACTCTGGAGACATATACCGAGGATCCTTGGCAAGTGAAACCCCTGTAGTTATCAAAAGGGTAAATTTGCATTCCGTAAAGAAAGAATCATACACGATAGAAATGGATTTATTCAGCAAGGTTTCACATACGAGATTGGTGCCACTTTTGGGTCACTGCTTGGAGCATGAGAGCGAGAAGCTTCTGGTTTACAAATACATGCCAAATGGAGACTTGGCTAGTTCCTTGCACAGGGTTACCAACTTAGGAGATGGCAATTTACAGTCCCTGGATTGGATTACAAGATTAAAAATTGCAATTGGAGCTGCTGAAATCCTAGCTTACCTACATCATGAATGCAGCCCGCCACTCGTTCACAGAGATGTTCAAGCTAGCAGTATACTTCTCGATGATAAATTTGAGGTCCGGCTTGGAAGCTGGAGTGAGGTCCGTGTGCAAGAAGGGGATGGTAACCCAAATGTGATCACGAGATTGTTGCGGAAACAACAAACCTTGGAACAAAGCCCTTATGCCTCGGCATCAGCTGCTTGCTCATATGATGTATACTGTTTTGGGAAGGTTTTGCTTGAGCTTGTAACGGGTAAGCTTGGCATCAGCAAATCTGATGATTCGTCTACAAGGGAGTTGTTGGATCACACGGTGCGCTACATCAGCATATATGACAAGGAACTGTTGAATAAGATTGTTGACCCATCTCTGATAGTAGATGAAGATCTATTGGAAGAAGTTTGGGCCATGGCAATCGTGGCCAGGTCCTGCCTCAACCCCAAGCCGTCTAAGCGTCCCCCGATGAAATACATCCTTAAGGCATTGGAAAACCCTCTAAAGGTGGTGAGGGAAGAGAGTTCCAGCTCTGCAAGGCTGCGAACAGCTTCATCGAGAAGATCCTGGAGCACTGCTTTCTTTGGTAGCTGGCAGCAGAGCTCATCAGAGAGCGCCACCGCTCCTGGCCACACAAGCAGAGAGAGTATCAGTGGCTTAAAACGGGAATCCTCTGCATCACACAAAAGGTTATCAAGTGAAATATTCCCTGAGCCAATTGAAATACAAGATCTCGAGCGACAAGATGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

899

Amino Acids

97.85

Weight (kDa)

6.5

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 78 2.6e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 136 - 217 3.8e-07 Leucine-rich repeat region
LRR_8 PF13855 162 - 219 1.6e-11 Leucine rich repeat
LRR_14 PF23598 228 - 338 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 234 - 293 1.6e-08 Leucine rich repeat
LRR_8 PF13855 297 - 342 1.9e-08 Leucine rich repeat
Pkinase PF00069 536 - 809 9.4e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 537 - 811 1.9e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1610
Acc16I TGCGCA 1 cut(s) 102
AccB1I GGYRCC 3 cut(s) 53, 971, 1759
AccBSI CCGCTC 2 cut(s) 133, 2564
AccI GTMKAC 3 cut(s) 1999, 2163, 2234
AccII CGCG 1 cut(s) 368
AciI CCGC 5 cut(s) 133, 1015, 1964, 2098, 2562
AclWI GGATC 4 cut(s) 1638, 1651, 2259, 2499
AcoI YGGCCR 4 cut(s) 241, 397, 2367, 2569
AcsI RAATTY 6 cut(s) 564, 655, 950, 1130, 1684, 2015
AcyI GRCGYC 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 2260
AfaI GTAC 2 cut(s) 1230, 1416
AfiI CCNNNNNNNGG 9 cut(s) 273, 686, 699, 1046, 1171, 1769, 1851, 1893, 2446
AflII CTTAAG 2 cut(s) 704, 2423
AhdI GACNNNNNGTC 1 cut(s) 433
Alw21I GWGCWC 8 cut(s) 122, 153, 415, 426, 964, 1434, 2516, 2546
Alw26I GTCTC 5 cut(s) 173, 737, 1141, 1623, 1824
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 4 cut(s) 1638, 1651, 2259, 2499
AlwNI CAGNNNCTG 2 cut(s) 1888, 2376
Ama87I CYCGRG 2 cut(s) 282, 2675
ApaI GGGCCC 1 cut(s) 1306
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 7 cut(s) 103, 231, 1925, 1959, 2144, 2482, 2536
ApoI RAATTY 6 cut(s) 564, 655, 950, 1130, 1684, 2015
Asp700I GAANNNNTTC 2 cut(s) 694, 2491
AspLEI GCGC 6 cut(s) 56, 103, 370, 1073, 2265, 2558
AsuC2I CCSGG 3 cut(s) 304, 361, 772
AsuHPI GGTGA 4 cut(s) 325, 669, 727, 2464
AsuNHI GCTAGC 1 cut(s) 1991
AvaI CYCGRG 2 cut(s) 282, 2675
AvaII GGWCC 7 cut(s) 216, 378, 597, 884, 2023, 2047, 2373
AxyI CCTNAGG 1 cut(s) 431
BaeGI GKGCMC 2 cut(s) 415, 1306
BalI TGGCCA 3 cut(s) 399, 2369, 2571
BamHI GGATCC 1 cut(s) 1643
BanI GGYRCC 3 cut(s) 53, 971, 1759
BanII GRGCYC 5 cut(s) 46, 122, 153, 1306, 2546
BauI CACGAG 1 cut(s) 2086
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 8 cut(s) 122, 153, 415, 426, 964, 1434, 2516, 2546
BbvI GCAGC 7 cut(s) 115, 243, 1912, 1971, 2131, 2469, 2548
BccI CCATC 5 cut(s) 850, 950, 1865, 2060, 2320
BceAI ACGGC 5 cut(s) 42, 47, 228, 256, 2377
BcgI CGANNNNNNTGC 4 cut(s) 1741, 1775, 2077, 2111
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 2082
BcnI CCSGG 3 cut(s) 304, 361, 772
BcoDI GTCTC 5 cut(s) 173, 737, 1141, 1623, 1824
BfaI CTAG 6 cut(s) 1059, 1307, 1331, 1839, 1937, 1992
BfmI CTRYAG 2 cut(s) 1220, 1665
BfoI RGCGCY 3 cut(s) 57, 1074, 2559
BfrI CTTAAG 2 cut(s) 704, 2423
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 3 cut(s) 1522, 2332, 2671
BisI GCNGC 7 cut(s) 104, 232, 1926, 1960, 2145, 2483, 2537
BlpI GCTNAGC 1 cut(s) 1317
BlsI GCNGC 7 cut(s) 105, 233, 1927, 1961, 2146, 2484, 2538
Bme18I GGWCC 7 cut(s) 216, 378, 597, 884, 2023, 2047, 2373
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 2 cut(s) 282, 2675
BmiI GGNNCC 9 cut(s) 55, 379, 598, 691, 973, 1303, 1304, 1645, 1761
BmrI ACTGGG 4 cut(s) 457, 531, 603, 984
BmsI GCATC 5 cut(s) 1522, 1544, 2147, 2220, 2627
BmtI GCTAGC 1 cut(s) 1995
BmuI ACTGGG 4 cut(s) 457, 531, 603, 984
BoxI GACNNNNGTC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 5 cut(s) 167, 401, 1647, 2059, 2530
Bpu10I CCTNAGC 1 cut(s) 2652
Bpu1102I GCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 1 cut(s) 2207
BpuMI CCSGG 3 cut(s) 304, 361, 772
Bsa29I ATCGAT 2 cut(s) 1143, 1530
BsaAI YACGTR 1 cut(s) 1560
BsaBI GATNNNNATC 1 cut(s) 2331
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 173
BsaWI WCCGGW 4 cut(s) 153, 218, 266, 748
BsaXI ACNNNNNCTCC 8 cut(s) 135, 140, 165, 170, 844, 874, 887, 917
Bsc4I CCNNNNNNNGG 9 cut(s) 273, 686, 699, 1046, 1171, 1769, 1851, 1893, 2446
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
Bse8I GATNNNNATC 1 cut(s) 2331
BseCI ATCGAT 2 cut(s) 1143, 1530
BseGI GGATG 4 cut(s) 209, 861, 2071, 2418
BseJI GATNNNNATC 1 cut(s) 2331
BseLI CCNNNNNNNGG 9 cut(s) 273, 686, 699, 1046, 1171, 1769, 1851, 1893, 2446
BseMI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
BseMII CTCAG 5 cut(s) 184, 821, 929, 1331, 2643
BseRI GAGGAG 3 cut(s) 856, 865, 1560
BseSI GKGCMC 2 cut(s) 415, 1306
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 7 cut(s) 115, 243, 1912, 1971, 2131, 2469, 2548
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 2 cut(s) 137, 244
BshNI GGYRCC 3 cut(s) 53, 971, 1759
BshVI ATCGAT 2 cut(s) 1143, 1530
BsiEI CGRYCG 2 cut(s) 137, 244
BsiHKAI GWGCWC 8 cut(s) 122, 153, 415, 426, 964, 1434, 2516, 2546
BsiHKCI CYCGRG 2 cut(s) 282, 2675
BsiSI CCGG 9 cut(s) 154, 219, 267, 304, 360, 749, 772, 893, 2026
BslFI GGGAC 2 cut(s) 1870, 2388
BslI CCNNNNNNNGG 9 cut(s) 273, 686, 699, 1046, 1171, 1769, 1851, 1893, 2446
BsmAI GTCTC 5 cut(s) 173, 737, 1141, 1623, 1824
BsmFI GGGAC 2 cut(s) 1870, 2388
BsmI GAATGC 3 cut(s) 589, 1690, 1961
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 282, 2675
Bsp120I GGGCCC 1 cut(s) 1302
Bsp143I GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
Bsp1720I GCTNAGC 1 cut(s) 1317
Bsp19I CCATGG 1 cut(s) 2355
BspACI CCGC 5 cut(s) 133, 1015, 1964, 2098, 2562
BspCNI CTCAG 5 cut(s) 183, 822, 930, 1330, 2644
BspDI ATCGAT 2 cut(s) 1143, 1530
BspFNI CGCG 1 cut(s) 368
BspHI TCATGA 1 cut(s) 1951
BspLI GGNNCC 9 cut(s) 55, 379, 598, 691, 973, 1303, 1304, 1645, 1761
BspOI GCTAGC 1 cut(s) 1995
BspPI GGATC 4 cut(s) 1638, 1651, 2259, 2499
BspQI GCTCTTC 1 cut(s) 969
BspT107I GGYRCC 3 cut(s) 53, 971, 1759
BspTI CTTAAG 2 cut(s) 704, 2423
BspTNI GGTCTC 1 cut(s) 173
BsrBI CCGCTC 2 cut(s) 133, 2564
BsrDI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
BssMI GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
BssNAI GTATAC 2 cut(s) 2000, 2164
BssNI GRCGYC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 2086
BssT1I CCWWGG 4 cut(s) 1165, 1647, 2112, 2355
Bst1107I GTATAC 2 cut(s) 2000, 2164
Bst2BI CACGAG 1 cut(s) 2086
Bst4CI ACNGT 5 cut(s) 262, 1884, 2168, 2260, 2292
Bst6I CTCTTC 3 cut(s) 969, 1266, 2454
BstACI GRCGYC 1 cut(s) 54
BstAFI CTTAAG 2 cut(s) 704, 2423
BstAPI GCANNNNNTGC 1 cut(s) 2144
BstBAI YACGTR 1 cut(s) 1560
BstC8I GCNNGC 5 cut(s) 293, 1964, 1993, 2149, 2534
BstDEI CTNAG 9 cut(s) 170, 431, 791, 830, 938, 1317, 1864, 2397, 2652
BstDSI CCRYGG 1 cut(s) 2355
BstEII GGTNACC 2 cut(s) 1855, 2069
BstENI CCTNNNNNAGG 1 cut(s) 1849
BstF5I GGATG 4 cut(s) 209, 861, 2071, 2418
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 3 cut(s) 57, 1074, 2559
BstHHI GCGC 6 cut(s) 56, 103, 370, 1073, 2265, 2558
BstKTI GATC 9 cut(s) 574, 790, 1525, 1646, 2085, 2254, 2335, 2507, 2674
BstMAI GTCTC 5 cut(s) 173, 737, 1141, 1623, 1824
BstMBI GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
BstMCI CGRYCG 2 cut(s) 137, 244
BstMWI GCNNNNNNNGC 9 cut(s) 35, 240, 1452, 1922, 2144, 2271, 2366, 2398, 2577
BstNSI RCATGY 2 cut(s) 231, 1822
BstPAI GACNNNNGTC 1 cut(s) 221
BstPI GGTNACC 2 cut(s) 1855, 2069
BstSFI CTRYAG 2 cut(s) 1220, 1665
BstSLI GKGCMC 2 cut(s) 415, 1306
BstSNI TACGTA 1 cut(s) 1560
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 7 cut(s) 115, 243, 1912, 1971, 2131, 2469, 2548
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 5 cut(s) 1522, 1643, 2332, 2504, 2671
BstXI CCANNNNNNTGG 2 cut(s) 407, 470
BstYI RGATCY 5 cut(s) 1522, 1643, 2332, 2504, 2671
BstZ17I GTATAC 2 cut(s) 2000, 2164
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 2 cut(s) 1143, 1530
Bsu36I CCTNAGG 1 cut(s) 431
BsuI GTATCC 1 cut(s) 1021
BsuTUI ATCGAT 2 cut(s) 1143, 1530
BtgI CCRYGG 1 cut(s) 2355
BtsCI GGATG 4 cut(s) 209, 861, 2071, 2418
BtsI GCAGTG 2 cut(s) 1774, 2514
BtsIMutI CAGTG 6 cut(s) 249, 320, 1600, 1774, 2514, 2599
Cac8I GCNNGC 5 cut(s) 293, 1964, 1993, 2149, 2534
CaiI CAGNNNCTG 2 cut(s) 1888, 2376
CciI TCATGA 1 cut(s) 1951
CfoI GCGC 6 cut(s) 56, 103, 370, 1073, 2265, 2558
ClaI ATCGAT 2 cut(s) 1143, 1530
CseI GACGC 2 cut(s) 429, 2390
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 2 cut(s) 1229, 1415
CviAII CATG 8 cut(s) 127, 204, 228, 1616, 1787, 1819, 1952, 2356
CviQI GTAC 2 cut(s) 1229, 1415
DdeI CTNAG 9 cut(s) 170, 431, 791, 830, 938, 1317, 1864, 2397, 2652
DinI GGCGCC 1 cut(s) 55
DpnI GATC 9 cut(s) 573, 789, 1524, 1645, 2084, 2253, 2334, 2506, 2673
DpnII GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
DraIII CACNNNGTG 1 cut(s) 2260
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 4 cut(s) 241, 397, 2367, 2569
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 969, 1266, 2454
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 3 cut(s) 969, 1266, 2454
Ecl136II GAGCTC 3 cut(s) 120, 151, 2544
EclXI CGGCCG 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 1560
Eco130I CCWWGG 4 cut(s) 1165, 1647, 2112, 2355
Eco24I GRGCYC 5 cut(s) 46, 122, 153, 1306, 2546
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 7 cut(s) 216, 378, 597, 884, 2023, 2047, 2373
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 3 cut(s) 120, 151, 2544
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 2 cut(s) 282, 2675
Eco91I GGTNACC 2 cut(s) 1855, 2069
EcoICRI GAGCTC 3 cut(s) 120, 151, 2544
EcoNI CCTNNNNNAGG 1 cut(s) 1849
EcoO109I RGGNCCY 3 cut(s) 1302, 1303, 2373
EcoO65I GGTNACC 2 cut(s) 1855, 2069
EcoT14I CCWWGG 4 cut(s) 1165, 1647, 2112, 2355
EcoT22I ATGCAT 1 cut(s) 1537
EcoT38I GRGCYC 5 cut(s) 46, 122, 153, 1306, 2546
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 4 cut(s) 1165, 1647, 2112, 2355
FaeI CATG 8 cut(s) 130, 207, 231, 1619, 1790, 1822, 1955, 2359
FalI AAGNNNNNCTT 2 cut(s) 1590, 1622
FaqI GGGAC 2 cut(s) 1870, 2388
FatI CATG 8 cut(s) 126, 203, 227, 1615, 1786, 1818, 1951, 2355
FauI CCCGC 2 cut(s) 1022, 1971
FauNDI CATATG 1 cut(s) 2155
FbaI TGATCA 1 cut(s) 2082
FblI GTMKAC 3 cut(s) 1999, 2163, 2234
Fnu4HI GCNGC 7 cut(s) 104, 232, 1926, 1960, 2145, 2483, 2537
FokI GGATG 4 cut(s) 196, 868, 2078, 2405
FriOI GRGCYC 5 cut(s) 46, 122, 153, 1306, 2546
Fsp4HI GCNGC 7 cut(s) 104, 232, 1926, 1960, 2145, 2483, 2537
FspBI CTAG 6 cut(s) 1059, 1307, 1331, 1839, 1937, 1992
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 6 cut(s) 55, 102, 369, 1072, 2264, 2557
GluI GCNGC 7 cut(s) 104, 232, 1926, 1960, 2145, 2483, 2537
GsuI CTGGAG 5 cut(s) 167, 401, 1647, 2059, 2530
HaeII RGCGCY 3 cut(s) 57, 1074, 2559
HapII CCGG 9 cut(s) 154, 219, 267, 304, 360, 749, 772, 893, 2026
HgaI GACGC 2 cut(s) 429, 2390
HhaI GCGC 6 cut(s) 56, 103, 370, 1073, 2265, 2558
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 8 cut(s) 130, 207, 231, 1619, 1790, 1822, 1955, 2359
Hin6I GCGC 6 cut(s) 54, 101, 368, 1071, 2263, 2556
HinP1I GCGC 6 cut(s) 54, 101, 368, 1071, 2263, 2556
HincII GTYRAC 1 cut(s) 2308
HindII GTYRAC 1 cut(s) 2308
HindIII AAGCTT 3 cut(s) 629, 1799, 2204
HpaII CCGG 9 cut(s) 154, 219, 267, 304, 360, 749, 772, 893, 2026
HphI GGTGA 4 cut(s) 325, 669, 727, 2464
Hpy166II GTNNAC 8 cut(s) 413, 1391, 1810, 1975, 2000, 2164, 2235, 2308
Hpy8I GTNNAC 8 cut(s) 413, 1391, 1810, 1975, 2000, 2164, 2235, 2308
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 7 cut(s) 543, 615, 875, 1490, 1518, 2054, 2170
HpyCH4III ACNGT 5 cut(s) 262, 1884, 2168, 2260, 2292
HpyCH4IV ACGT 2 cut(s) 1465, 1559
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 240, 1452, 1922, 2144, 2271, 2366, 2398, 2577
HpyF3I CTNAG 9 cut(s) 170, 431, 791, 830, 938, 1317, 1864, 2397, 2652
HpySE526I ACGT 2 cut(s) 1465, 1559
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 8 cut(s) 130, 207, 231, 1619, 1790, 1822, 1955, 2359
HspAI GCGC 6 cut(s) 54, 101, 368, 1071, 2263, 2556
KasI GGCGCC 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 2082
Kzo9I GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 9 cut(s) 125, 130, 148, 156, 1437, 1783, 1922, 2511, 2569
Lsp1109I GCAGC 7 cut(s) 115, 243, 1912, 1971, 2131, 2469, 2548
LweI GCATC 5 cut(s) 1522, 1544, 2147, 2220, 2627
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 6 cut(s) 1059, 1307, 1331, 1839, 1937, 1992
MaeII ACGT 2 cut(s) 1465, 1559
MalI GATC 9 cut(s) 573, 789, 1524, 1645, 2084, 2253, 2334, 2506, 2673
MbiI CCGCTC 2 cut(s) 133, 2564
MboI GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
MboII GAAGA 9 cut(s) 150, 642, 956, 1193, 1283, 2342, 2354, 2471, 2514
MfeI CAATTG 2 cut(s) 1917, 2658
MflI RGATCY 5 cut(s) 1522, 1643, 2332, 2504, 2671
MlsI TGGCCA 3 cut(s) 399, 2369, 2571
MluNI TGGCCA 3 cut(s) 399, 2369, 2571
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 4 cut(s) 231, 919, 949, 1386
MmeI TCCRAC 4 cut(s) 147, 540, 923, 2229
Mox20I TGGCCA 3 cut(s) 399, 2369, 2571
Mph1103I ATGCAT 1 cut(s) 1537
MroXI GAANNNNTTC 2 cut(s) 694, 2491
MscI TGGCCA 3 cut(s) 399, 2369, 2571
MseI TTAA 7 cut(s) 71, 705, 1283, 1361, 1907, 2424, 2600
MslI CAYNNNNRTG 1 cut(s) 1980
Msp20I TGGCCA 3 cut(s) 399, 2369, 2571
MspA1I CMGCKG 2 cut(s) 922, 2144
MspCI CTTAAG 2 cut(s) 704, 2423
MspI CCGG 9 cut(s) 154, 219, 267, 304, 360, 749, 772, 893, 2026
MunI CAATTG 2 cut(s) 1917, 2658
Mva1269I GAATGC 3 cut(s) 589, 1690, 1961
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 9 cut(s) 35, 240, 1452, 1922, 2144, 2271, 2366, 2398, 2577
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 3 cut(s) 304, 361, 772
NcoI CCATGG 1 cut(s) 2355
NdeI CATATG 1 cut(s) 2155
NdeII GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
NheI GCTAGC 1 cut(s) 1991
NlaIII CATG 8 cut(s) 130, 207, 231, 1619, 1790, 1822, 1955, 2359
NlaIV GGNNCC 9 cut(s) 55, 379, 598, 691, 973, 1303, 1304, 1645, 1761
NmeAIII GCCGAG 2 cut(s) 1374, 2114
NmuCI GTSAC 4 cut(s) 223, 247, 981, 1772
NsbI TGCGCA 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 1537
NspI RCATGY 2 cut(s) 231, 1822
PaeR7I CTCGAG 1 cut(s) 2675
PagI TCATGA 1 cut(s) 1951
PciSI GCTCTTC 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PctI GAATGC 3 cut(s) 589, 1690, 1961
PdmI GAANNNNTTC 2 cut(s) 694, 2491
PfoI TCCNGGA 2 cut(s) 212, 2507
PkrI GCNGC 7 cut(s) 105, 233, 1927, 1961, 2146, 2484, 2538
PleI GAGTC 4 cut(s) 230, 918, 948, 1386
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 4 cut(s) 230, 918, 948, 1386
Ppu21I YACGTR 1 cut(s) 1560
PpuMI RGGWCCY 1 cut(s) 2373
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 1610
Psp124BI GAGCTC 3 cut(s) 122, 153, 2546
Psp5II RGGWCCY 1 cut(s) 2373
PspEI GGTNACC 2 cut(s) 1855, 2069
PspN4I GGNNCC 9 cut(s) 55, 379, 598, 691, 973, 1303, 1304, 1645, 1761
PspOMI GGGCCC 1 cut(s) 1302
PspPPI RGGWCCY 1 cut(s) 2373
PstNI CAGNNNCTG 2 cut(s) 1888, 2376
PsuI RGATCY 5 cut(s) 1522, 1643, 2332, 2504, 2671
PvuII CAGCTG 2 cut(s) 922, 2144
RsaI GTAC 2 cut(s) 1230, 1416
RsaNI GTAC 2 cut(s) 1229, 1415
RseI CAYNNNNRTG 1 cut(s) 1980
SacI GAGCTC 3 cut(s) 122, 153, 2546
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 7 cut(s) 71, 705, 1283, 1361, 1907, 2424, 2600
SatI GCNGC 7 cut(s) 104, 232, 1926, 1960, 2145, 2483, 2537
Sau3AI GATC 9 cut(s) 571, 787, 1522, 1643, 2082, 2251, 2332, 2504, 2671
SchI GAGTC 4 cut(s) 231, 919, 949, 1386
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 5 cut(s) 1522, 1544, 2147, 2220, 2627
SfcI CTRYAG 2 cut(s) 1220, 1665
SfoI GGCGCC 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 2675
SinI GGWCC 7 cut(s) 216, 378, 597, 884, 2023, 2047, 2373
SlaI CTCGAG 1 cut(s) 2675
SmiMI CAYNNNNRTG 1 cut(s) 1980
SmlI CTYRAG 4 cut(s) 704, 2186, 2423, 2675
SmoI CTYRAG 4 cut(s) 704, 2186, 2423, 2675
SnaBI TACGTA 1 cut(s) 1560
SsiI CCGC 5 cut(s) 133, 1015, 1964, 2098, 2562
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 1 cut(s) 2646
SspMI CTAG 6 cut(s) 1059, 1307, 1331, 1839, 1937, 1992
SstI GAGCTC 3 cut(s) 122, 153, 2546
StyI CCWWGG 4 cut(s) 1165, 1647, 2112, 2355
TaaI ACNGT 5 cut(s) 262, 1884, 2168, 2260, 2292
TaiI ACGT 2 cut(s) 1468, 1562
Tru1I TTAA 7 cut(s) 71, 705, 1283, 1361, 1907, 2424, 2600
Tru9I TTAA 7 cut(s) 71, 705, 1283, 1361, 1907, 2424, 2600
TscAI CASTG 6 cut(s) 256, 327, 1600, 1781, 2521, 2599
TseFI GTSAC 4 cut(s) 223, 247, 981, 1772
TseI GCWGC 7 cut(s) 103, 231, 1925, 1959, 2144, 2482, 2536
Tsp45I GTSAC 4 cut(s) 223, 247, 981, 1772
TspDTI ATGAA 9 cut(s) 17, 198, 1284, 1390, 1542, 1968, 2343, 2426, 2484
TspGWI ACGGA 3 cut(s) 301, 1684, 2039
TspRI CASTG 6 cut(s) 256, 327, 1600, 1781, 2521, 2599
Vha464I CTTAAG 2 cut(s) 704, 2423
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 7 cut(s) 216, 378, 597, 884, 2023, 2047, 2373
XagI CCTNNNNNAGG 1 cut(s) 1849
XapI RAATTY 6 cut(s) 564, 655, 950, 1130, 1684, 2015
XceI RCATGY 2 cut(s) 231, 1822
XcmI CCANNNNNNNNNTGG 2 cut(s) 210, 2362
XhoI CTCGAG 1 cut(s) 2675
XmiI GTMKAC 3 cut(s) 1999, 2163, 2234
XmnI GAANNNNTTC 2 cut(s) 694, 2491
XspI CTAG 6 cut(s) 1059, 1307, 1331, 1839, 1937, 1992
Zsp2I ATGCAT 1 cut(s) 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.