RchiOBHm_Chr6g0264451

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002531
Physical Location & Seq
Reverse (-)
19594878 .. 19602272
7395 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23718

Sequence Viewer

Length: 2646 bp
ATGAGAGCCATGGTGGATCAAAGGAGCATAGTATGGTGTTTCTTGTTGTTGTGGGCGGCGTTGGTGTTTGAGCCCACATTTCAGCAACCGAGTCAGCAGCCACTGAGTTCACACGCCGACCGAGTTGCTCTGGTTCAGCTCAGATCGTCTTTGGGTTTGAGGGGCAGGGACTGGCCCATTAAGTCAGACCCTTGCTTCGTTTGGAAGGGTGTTGAATGCAGTAATGGTAGAGTTGTCGGGATTAACGTTTCTGGGTTTAGAAGAACTAGACTTGGGAGTCAAAACCCACAATTCTCAGTCGATGCTTTGGCCAATTTGACCCTTTTGAGTTCTTTCAATGCCTCCAATTTCTTGCTTCCGGGTTCCATTCCTGACTGGTTTGGCCAGAGGCTCGGGTCTCTGCGTGTTCTTGATCTCACTTCTTGTTCTGTGAATGGTCCTATTCCATTGAGTCTTGGAAATTCTACCAATCTGACTGGTCTGTCTTTGTCTCGTAATAACCTTAATGGGACAATTCCTGCTAGTTTGAGCAACTTGGTAAGCCTTTTGGTCCTTGACCTCTCTCAAAATCGGCTTACTGGATCCATTCCCACATCTTTCGGGAATCTTGGGAACCTTTCTGTTCTTGACATTTCTGGGAATTTCTTAACCGGGTCAATTCCTCCGGGCATTGGGAACCTCTTGAAGCTTCAATCTCTGAATCTTTCCAGCAACATGTTGTCTTCTTCCATTCCTCCTCAACTTGGGGGCCTTGATACCTTGGTTGACCTTGATCTCAGCTCCAATCTATTGGTAGGTCTGCTGCCTTCGGGTTTGAGTAACTTGCAGAGAATTGTAGTTGCGGACAATTTTCTCAGTGGTTCTGTGGATGTGCTCTGGTCAATGCCTCAATTGAGTTTTCTTGATGTGGCTGGAAACAATTTCACTGGTTTGCTGCCCAATTCCAGCTCGAATGCTAATGCCACTGCAGCAGTATTCAATATTTCTCAAAATTCGTTTTATGGGCCTATTACACCATTAGTTGGAAAGTTTGGTGTCATTGATCTTTCAGGAAACTATTTTGAAGGTGAGATACCAGGTTATGTTGGCACCAATGCATCTCTTGACAGCAATTGTCTCCAAAATTTGAAGAATCAGAGGACTTTGGCAGAATGTTCATCGTTTTATGCTAATAGGGGTTTGAGTTTTGATAATTTTGGGCAGCCAAACTCTACACAACCTCCTGCAGCCGCACCACCTGGGAAGAAAAGTAATAAAAATGTGATCATATTGGCATCAGTTTTAGGAGGAGTTGGTTTGATTGTGGTTTTGGTCTTGTTTTTAGTAATAGTAGTCCTATGTTCACGCAAGCGGGGAGCTACAACTCAAAGGGGGGTTGGTGTGGGGTCAGTTCCTGCAGGCAGCAGTCCCCCACCAGCTGGAGCATCGGTTAACTTTTCTAGCGTAGGAGATGCATTCACATATCAGCAGCTGCTTCAAGCTACAGGTGACTTCAACAATACAAACTTAATGAAAAATGGCCACACAGGGGATCTCTTCAGGGGTGTCTTGGAAAATGGGATCCCTGTGGTTATTAAAAGGATTGATTTACGTTCGATTAAAAAGGAAGCATACCTGCTGGAGTTGGATTTCTTTAGTAAGGTGTCAAGTACAAGATTTGTTCCCTTTCTGGGACACTGTTTAGAGAATGAGAACGAGAAGTTCATGGTGTACAAATACATGCCAAATGGGGACCTATCAAGTTCTCTGTACAAGAAAACCAACACCCTGGATGATACTTTACAGTCATTGGATTGGATTACTAGATTAAAGATTGCATTAGGTGCTGCGGAGGGTCTCACCTATCTGCATCATGAGTGCGATCCACCCCTCGTGCACAGAGATGTGCAAGCAAGCAGTGTCCTTCTTGATGATAAATTTGAAGTGCGGCTAGGAAGCCTGAGTGATGTCTGTGCTCAAGAAGGAGATACTCATCAAAGCAGGATTACTAGGTTGCTGCGGTTGCCACAGTCATCCGAACAAGGTGCTTCTGGTTCATCGACAGCATCATGTGCCTATGATGTTTATTGTTTCGGGAAGGTATTACTTGAACTGGTAACAGGTAAATTGGGCATCAGTGCTTCCAGTGAATCCCAATTGAAGGAATCGTTAGATGACATACTTCCCTACATCAGTATATATGACAAGGAACTTGTGACAAAAATTGTGGATCCATCTTTGATTGTGGATGAGGACTTATTAGAGGAAGTGTGGGCTATGGCCGTCGTGGCCAGGTCCTGCCTAAATCCCAAGCCCTCAAGGCGCCCGCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTGTTAAGGTAGTAAGGGAAGAGAGTACTGGCTCTGCAAGGCTGAGAAAAGCTTCTTCTCGAGGGTCTTGGAATGCTGCTGTCTTTGGTAGCTGGCGCAGCACATCTGAGGTAGTGGTTATTCCAGGAGCCTCAGGTACCAAAGCTGAAGGAGTGAGTGGTTTGAAGCATTCAGCAACCACCGGTTCGCAGGGAAGTGGCCAAAATGGTGGGGGTGAACATTCATCCTCACGTAGACGGCATTCTAGGGATATTTTCCCTGAACCATCTGGTGTACAAGATGTAGAGAGATTTGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

881

Amino Acids

94.62

Weight (kDa)

7.11

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 133 - 279 2.2e-13 Leucine-rich repeat region
LRR_8 PF13855 157 - 214 1.9e-08 Leucine rich repeat
LRR_LRWD1 PF23211 226 - 310 5.7e-07 Leucine-rich repeat and WD repeat-containing protein 1, LRR
PK_Tyr_Ser-Thr PF07714 504 - 777 9.2e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 506 - 775 5.2e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 276, 481
Acc36I ACCTGC 1 cut(s) 1623
Acc65I GGTACC 1 cut(s) 2477
AccB1I GGYRCC 3 cut(s) 1088, 2301, 2477
AccB7I CCANNNNNTGG 2 cut(s) 1022, 1418
AccI GTMKAC 1 cut(s) 2575
AciI CCGC 8 cut(s) 56, 842, 1230, 1351, 1829, 1927, 1999, 2306
AclI AACGTT 1 cut(s) 246
AclWI GGATC 9 cut(s) 24, 576, 589, 1539, 1555, 1568, 1856, 2204, 2217
AcoI YGGCCR 6 cut(s) 309, 382, 1519, 2259, 2268, 2539
AcsI RAATTY 5 cut(s) 460, 640, 991, 1123, 1916
AcuI CTGAAG 2 cut(s) 1522, 2508
AcyI GRCGYC 1 cut(s) 2302
AfaI GTAC 6 cut(s) 1651, 1712, 1751, 2368, 2479, 2616
AfiI CCNNNNNNNGG 8 cut(s) 671, 743, 1022, 1418, 1528, 1670, 2140, 2347
AflIII ACRYGT 1 cut(s) 714
AgeI ACCGGT 1 cut(s) 2522
AjnI CCWGG 6 cut(s) 1075, 1237, 1767, 2270, 2464, 2637
Alw21I GWGCWC 3 cut(s) 876, 1878, 1957
Alw26I GTCTC 4 cut(s) 402, 495, 1121, 1841
Alw44I GTGCAC 1 cut(s) 1874
AlwI GGATC 9 cut(s) 24, 576, 589, 1539, 1555, 1568, 1856, 2204, 2217
AlwNI CAGNNNCTG 5 cut(s) 103, 171, 1394, 1471, 2277
Ama87I CYCGRG 2 cut(s) 392, 2400
AoxI GGCC 9 cut(s) 173, 309, 382, 748, 1004, 1519, 2259, 2268, 2539
ApaLI GTGCAC 1 cut(s) 1874
ApoI RAATTY 5 cut(s) 460, 640, 991, 1123, 1916
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AsiGI ACCGGT 1 cut(s) 2522
Asp700I GAANNNNTTC 1 cut(s) 2392
Asp718I GGTACC 1 cut(s) 2477
AspLEI GCGC 2 cut(s) 2304, 2439
AspS9I GGNCC 7 cut(s) 174, 437, 550, 748, 1004, 1732, 2274
AsuC2I CCSGG 3 cut(s) 360, 652, 666
AsuHPI GGTGA 4 cut(s) 1079, 1499, 1831, 2567
AvaI CYCGRG 2 cut(s) 392, 2400
AvaII GGWCC 4 cut(s) 437, 550, 1732, 2274
AxyI CCTNAGG 1 cut(s) 2473
BaeGI GKGCMC 1 cut(s) 1878
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BalI TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
BamHI GGATCC 3 cut(s) 581, 1560, 2209
BanI GGYRCC 3 cut(s) 1088, 2301, 2477
BanII GRGCYC 1 cut(s) 75
BarI GAAGNNNNNNTAC 2 cut(s) 1056, 1088
BauI CACGAG 1 cut(s) 1871
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 3 cut(s) 876, 1878, 1957
BccI CCATC 2 cut(s) 2221, 2614
BceAI ACGGC 2 cut(s) 2246, 2594
BcgI CGANNNNNNTGC 4 cut(s) 107, 141, 2006, 2040
BciT130I CCWGG 6 cut(s) 1077, 1239, 1769, 2272, 2466, 2639
BclI TGATCA 1 cut(s) 1263
BcnI CCSGG 3 cut(s) 360, 652, 666
BcoDI GTCTC 4 cut(s) 402, 495, 1121, 1841
BfaI CTAG 8 cut(s) 267, 522, 1440, 1803, 1931, 1989, 2586, 2644
BfmI CTRYAG 4 cut(s) 966, 1224, 1395, 1482
BfoI RGCGCY 1 cut(s) 2305
BfuAI ACCTGC 1 cut(s) 1623
BglI GCCNNNNNGGC 2 cut(s) 2267, 2299
BmcAI AGTACT 1 cut(s) 2368
Bme1390I CCNGG 9 cut(s) 360, 652, 666, 1077, 1239, 1769, 2272, 2466, 2639
Bme18I GGWCC 4 cut(s) 437, 550, 1732, 2274
BmeT110I CYCGRG 2 cut(s) 392, 2400
BmgT120I GGNCC 7 cut(s) 174, 437, 550, 748, 1004, 1732, 2274
BmrFI CCNGG 9 cut(s) 360, 652, 666, 1077, 1239, 1769, 2272, 2466, 2639
BmsI GCATC 8 cut(s) 292, 1106, 1283, 1433, 1441, 1858, 2054, 2121
BpiI GAAGAC 1 cut(s) 714
BpmI CTGGAG 2 cut(s) 1440, 1640
BpuEI CTTGAG 3 cut(s) 1941, 2281, 2309
BpuMI CCSGG 3 cut(s) 360, 652, 666
BsaAI YACGTR 1 cut(s) 2573
BsaBI GATNNNNATC 1 cut(s) 1971
BsaHI GRCGYC 1 cut(s) 2302
BsaI GGTCTC 2 cut(s) 402, 1841
BsaJI CCNNGG 4 cut(s) 9, 759, 1238, 1767
BsaWI WCCGGW 1 cut(s) 2522
BsaXI ACNNNNNCTCC 4 cut(s) 1204, 1234, 1413, 1443
Bsc4I CCNNNNNNNGG 8 cut(s) 671, 743, 1022, 1418, 1528, 1670, 2140, 2347
Bse118I RCCGGY 1 cut(s) 2522
Bse1I ACTGG 8 cut(s) 176, 380, 481, 583, 931, 2097, 2124, 2374
Bse21I CCTNAGG 1 cut(s) 2473
Bse8I GATNNNNATC 1 cut(s) 1971
BseBI CCWGG 6 cut(s) 1077, 1239, 1769, 2272, 2466, 2639
BseDI CCNNGG 4 cut(s) 9, 759, 1238, 1767
BseGI GGATG 5 cut(s) 874, 1777, 2012, 2233, 2564
BseJI GATNNNNATC 1 cut(s) 1971
BseLI CCNNNNNNNGG 8 cut(s) 671, 743, 1022, 1418, 1528, 1670, 2140, 2347
BseMII CTCAG 9 cut(s) 95, 154, 309, 790, 868, 1931, 2375, 2439, 2487
BseNI ACTGG 8 cut(s) 176, 380, 481, 583, 931, 2097, 2124, 2374
BseRI GAGGAG 2 cut(s) 726, 1302
BseSI GKGCMC 1 cut(s) 1878
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 9 cut(s) 175, 311, 384, 750, 1006, 1521, 2261, 2270, 2541
BshNI GGYRCC 3 cut(s) 1088, 2301, 2477
BshTI ACCGGT 1 cut(s) 2522
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 3 cut(s) 876, 1878, 1957
BsiHKCI CYCGRG 2 cut(s) 392, 2400
BsiSI CCGG 4 cut(s) 359, 651, 665, 2523
BslFI GGGAC 5 cut(s) 182, 523, 1392, 1686, 1745
BslI CCNNNNNNNGG 8 cut(s) 671, 743, 1022, 1418, 1528, 1670, 2140, 2347
BsmAI GTCTC 4 cut(s) 402, 495, 1121, 1841
BsmFI GGGAC 5 cut(s) 182, 523, 1392, 1686, 1745
BsmI GAATGC 6 cut(s) 221, 958, 1454, 2419, 2509, 2581
BsnI GGCC 9 cut(s) 175, 311, 384, 750, 1006, 1521, 2261, 2270, 2541
Bso31I GGTCTC 2 cut(s) 402, 1841
BsoBI CYCGRG 2 cut(s) 392, 2400
Bsp1286I GDGCHC 4 cut(s) 75, 876, 1878, 1957
Bsp1407I TGTACA 3 cut(s) 1710, 1749, 2614
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 8 cut(s) 56, 842, 1230, 1351, 1829, 1927, 1999, 2306
BspANI GGCC 9 cut(s) 175, 311, 384, 750, 1006, 1521, 2261, 2270, 2541
BspCNI CTCAG 9 cut(s) 96, 153, 308, 789, 867, 1932, 2376, 2440, 2486
BspHI TCATGA 1 cut(s) 1852
BspMAI CTGCAG 3 cut(s) 970, 1228, 1399
BspMI ACCTGC 1 cut(s) 1623
BspPI GGATC 9 cut(s) 24, 576, 589, 1539, 1555, 1568, 1856, 2204, 2217
BspT107I GGYRCC 3 cut(s) 1088, 2301, 2477
BspTNI GGTCTC 2 cut(s) 402, 1841
BsrFI RCCGGY 1 cut(s) 2522
BsrGI TGTACA 3 cut(s) 1710, 1749, 2614
BsrI ACTGG 8 cut(s) 176, 380, 481, 583, 931, 2097, 2124, 2374
BssAI RCCGGY 1 cut(s) 2522
BssECI CCNNGG 4 cut(s) 9, 759, 1238, 1767
BssNI GRCGYC 1 cut(s) 2302
BssSI CACGAG 1 cut(s) 1871
BssT1I CCWWGG 2 cut(s) 9, 759
Bst2BI CACGAG 1 cut(s) 1871
Bst2UI CCWGG 6 cut(s) 1077, 1239, 1769, 2272, 2466, 2639
Bst4CI ACNGT 3 cut(s) 1679, 1785, 2010
Bst6I CTCTTC 2 cut(s) 1541, 2355
BstACI GRCGYC 1 cut(s) 2302
BstAPI GCANNNNNTGC 2 cut(s) 1823, 2051
BstAUI TGTACA 3 cut(s) 1710, 1749, 2614
BstBAI YACGTR 1 cut(s) 2573
BstC8I GCNNGC 6 cut(s) 1349, 1399, 1890, 1894, 2306, 2435
BstDEI CTNAG 9 cut(s) 104, 140, 295, 776, 854, 1940, 2384, 2448, 2473
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 5 cut(s) 874, 1777, 2012, 2233, 2564
BstH2I RGCGCY 1 cut(s) 2305
BstHHI GCGC 2 cut(s) 2304, 2439
BstMAI GTCTC 4 cut(s) 402, 495, 1121, 1841
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 7 cut(s) 968, 1823, 2002, 2051, 2267, 2299, 2439
BstNI CCWGG 6 cut(s) 1077, 1239, 1769, 2272, 2466, 2639
BstNSI RCATGY 2 cut(s) 718, 1723
BstSCI CCNGG 9 cut(s) 358, 650, 664, 1075, 1237, 1767, 2270, 2464, 2637
BstSFI CTRYAG 4 cut(s) 966, 1224, 1395, 1482
BstSLI GKGCMC 1 cut(s) 1878
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 4 cut(s) 581, 1531, 1560, 2209
BstXI CCANNNNNNTGG 3 cut(s) 790, 1768, 2549
BstYI RGATCY 4 cut(s) 581, 1531, 1560, 2209
Bsu36I CCTNAGG 1 cut(s) 2473
BsuRI GGCC 9 cut(s) 175, 311, 384, 750, 1006, 1521, 2261, 2270, 2541
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 874, 1777, 2012, 2233, 2564
BtsI GCAGTG 2 cut(s) 963, 1903
BtsIMutI CAGTG 8 cut(s) 101, 862, 924, 963, 1675, 1903, 2122, 2131
BveI ACCTGC 1 cut(s) 1623
Cac8I GCNNGC 6 cut(s) 1349, 1399, 1890, 1894, 2306, 2435
CaiI CAGNNNCTG 5 cut(s) 103, 171, 1394, 1471, 2277
CciI TCATGA 1 cut(s) 1852
CfoI GCGC 2 cut(s) 2304, 2439
Cfr10I RCCGGY 1 cut(s) 2522
Cfr13I GGNCC 7 cut(s) 174, 437, 550, 748, 1004, 1732, 2274
CsiI ACCWGGT 1 cut(s) 1075
Csp6I GTAC 6 cut(s) 1650, 1711, 1750, 2367, 2478, 2615
CspAI ACCGGT 1 cut(s) 2522
CspCI CAANNNNNGTGG 2 cut(s) 2187, 2222
CviAII CATG 6 cut(s) 10, 715, 1705, 1720, 1853, 2049
CviQI GTAC 6 cut(s) 1650, 1711, 1750, 2367, 2478, 2615
DdeI CTNAG 9 cut(s) 104, 140, 295, 776, 854, 1940, 2384, 2448, 2473
DinI GGCGCC 1 cut(s) 2303
DrdI GACNNNNNNGTC 2 cut(s) 276, 481
DseDI GACNNNNNNGTC 2 cut(s) 276, 481
EaeI YGGCCR 6 cut(s) 309, 382, 1519, 2259, 2268, 2539
Eam1104I CTCTTC 2 cut(s) 1541, 2355
EarI CTCTTC 2 cut(s) 1541, 2355
Eco130I CCWWGG 2 cut(s) 9, 759
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 2 cut(s) 402, 1841
Eco47I GGWCC 4 cut(s) 437, 550, 1732, 2274
Eco57I CTGAAG 2 cut(s) 1522, 2508
Eco81I CCTNAGG 1 cut(s) 2473
Eco88I CYCGRG 2 cut(s) 392, 2400
EcoO109I RGGNCCY 3 cut(s) 748, 1732, 2274
EcoRII CCWGG 6 cut(s) 1075, 1237, 1767, 2270, 2464, 2637
EcoT14I CCWWGG 2 cut(s) 9, 759
EcoT22I ATGCAT 2 cut(s) 1099, 1456
EcoT38I GRGCYC 1 cut(s) 75
EgeI GGCGCC 1 cut(s) 2303
EheI GGCGCC 1 cut(s) 2303
ErhI CCWWGG 2 cut(s) 9, 759
FaeI CATG 6 cut(s) 13, 718, 1708, 1723, 1856, 2052
FaqI GGGAC 5 cut(s) 182, 523, 1392, 1686, 1745
FatI CATG 6 cut(s) 9, 714, 1704, 1719, 1852, 2048
FauI CCCGC 2 cut(s) 1344, 2313
FbaI TGATCA 1 cut(s) 1263
FblI GTMKAC 1 cut(s) 2575
FokI GGATG 5 cut(s) 881, 1784, 1999, 2240, 2551
FriOI GRGCYC 1 cut(s) 75
FspBI CTAG 8 cut(s) 267, 522, 1440, 1803, 1931, 1989, 2586, 2644
GlaI GCGC 2 cut(s) 2303, 2438
GsuI CTGGAG 2 cut(s) 1440, 1640
HaeII RGCGCY 1 cut(s) 2305
HaeIII GGCC 9 cut(s) 175, 311, 384, 750, 1006, 1521, 2261, 2270, 2541
HapII CCGG 4 cut(s) 359, 651, 665, 2523
HhaI GCGC 2 cut(s) 2304, 2439
Hin1I GRCGYC 1 cut(s) 2302
Hin1II CATG 6 cut(s) 13, 718, 1708, 1723, 1856, 2052
Hin6I GCGC 2 cut(s) 2302, 2437
HinP1I GCGC 2 cut(s) 2302, 2437
HincII GTYRAC 2 cut(s) 766, 1432
HindII GTYRAC 2 cut(s) 766, 1432
HindIII AAGCTT 2 cut(s) 686, 2391
HinfI GANTC 8 cut(s) 91, 277, 451, 604, 700, 1132, 2129, 2144
HpaI GTTAAC 1 cut(s) 1432
HpaII CCGG 4 cut(s) 359, 651, 665, 2523
HphI GGTGA 4 cut(s) 1079, 1499, 1831, 2567
Hpy166II GTNNAC 9 cut(s) 110, 766, 1343, 1432, 1711, 1876, 2558, 2576, 2615
Hpy188I TCNGA 7 cut(s) 143, 187, 474, 699, 1137, 2017, 2449
Hpy8I GTNNAC 9 cut(s) 110, 766, 1343, 1432, 1711, 1876, 2558, 2576, 2615
Hpy99I CGWCG 1 cut(s) 2267
HpyAV CCTTC 8 cut(s) 199, 816, 1058, 1913, 1955, 2071, 2134, 2483
HpyCH4III ACNGT 3 cut(s) 1679, 1785, 2010
HpyCH4IV ACGT 3 cut(s) 246, 1591, 2572
HpyF10VI GCNNNNNNNGC 7 cut(s) 968, 1823, 2002, 2051, 2267, 2299, 2439
HpyF3I CTNAG 9 cut(s) 104, 140, 295, 776, 854, 1940, 2384, 2448, 2473
HpySE526I ACGT 3 cut(s) 246, 1591, 2572
Hsp92I GRCGYC 1 cut(s) 2302
Hsp92II CATG 6 cut(s) 13, 718, 1708, 1723, 1856, 2052
HspAI GCGC 2 cut(s) 2302, 2437
KasI GGCGCC 1 cut(s) 2301
KpnI GGTACC 1 cut(s) 2481
Ksp22I TGATCA 1 cut(s) 1263
KspAI GTTAAC 1 cut(s) 1432
LmnI GCTCC 5 cut(s) 24, 785, 1355, 1421, 2468
LweI GCATC 8 cut(s) 292, 1106, 1283, 1433, 1441, 1858, 2054, 2121
MabI ACCWGGT 1 cut(s) 1075
MaeI CTAG 8 cut(s) 267, 522, 1440, 1803, 1931, 1989, 2586, 2644
MaeII ACGT 3 cut(s) 246, 1591, 2572
MaeIII GTNAC 4 cut(s) 818, 1487, 2095, 2194
MboII GAAGA 8 cut(s) 273, 714, 717, 1141, 1255, 1528, 2372, 2388
MfeI CAATTG 3 cut(s) 890, 1111, 2135
MflI RGATCY 4 cut(s) 581, 1531, 1560, 2209
MhlI GDGCHC 4 cut(s) 75, 876, 1878, 1957
MlsI TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
MluNI TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
Mly113I GGCGCC 1 cut(s) 2302
MlyI GAGTC 3 cut(s) 100, 286, 460
MmeI TCCRAC 2 cut(s) 1003, 1605
Mox20I TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
Mph1103I ATGCAT 2 cut(s) 1099, 1456
MroXI GAANNNNTTC 1 cut(s) 2392
MscI TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
MslI CAYNNNNRTG 1 cut(s) 1881
Msp20I TGGCCA 5 cut(s) 311, 384, 1521, 2270, 2541
MspA1I CMGCKG 2 cut(s) 1418, 1471
MspI CCGG 4 cut(s) 359, 651, 665, 2523
MspR9I CCNGG 9 cut(s) 360, 652, 666, 1077, 1239, 1769, 2272, 2466, 2639
MunI CAATTG 3 cut(s) 890, 1111, 2135
Mva1269I GAATGC 6 cut(s) 221, 958, 1454, 2419, 2509, 2581
MvaI CCWGG 6 cut(s) 1077, 1239, 1769, 2272, 2466, 2639
MwoI GCNNNNNNNGC 7 cut(s) 968, 1823, 2002, 2051, 2267, 2299, 2439
NarI GGCGCC 1 cut(s) 2302
NciI CCSGG 3 cut(s) 360, 652, 666
NcoI CCATGG 1 cut(s) 9
NlaIII CATG 6 cut(s) 13, 718, 1708, 1723, 1856, 2052
NmuCI GTSAC 2 cut(s) 1487, 2194
NsiI ATGCAT 2 cut(s) 1099, 1456
NspI RCATGY 2 cut(s) 718, 1723
PaeR7I CTCGAG 1 cut(s) 2400
PagI TCATGA 1 cut(s) 1852
PciI ACATGT 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 6 cut(s) 221, 958, 1454, 2419, 2509, 2581
PdmI GAANNNNTTC 1 cut(s) 2392
PfeI GAWTC 5 cut(s) 604, 700, 1132, 2129, 2144
PflMI CCANNNNNTGG 2 cut(s) 1022, 1418
PfoI TCCNGGA 1 cut(s) 2464
PinAI ACCGGT 1 cut(s) 2522
PleI GAGTC 3 cut(s) 99, 285, 459
PluTI GGCGCC 1 cut(s) 2305
PpsI GAGTC 3 cut(s) 99, 285, 459
Ppu21I YACGTR 1 cut(s) 2573
PpuMI RGGWCCY 2 cut(s) 1732, 2274
PscI ACATGT 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 246
Psp5II RGGWCCY 2 cut(s) 1732, 2274
Psp6I CCWGG 6 cut(s) 1075, 1237, 1767, 2270, 2464, 2637
PspGI CCWGG 6 cut(s) 1075, 1237, 1767, 2270, 2464, 2637
PspPI GGNCC 7 cut(s) 174, 437, 550, 748, 1004, 1732, 2274
PspPPI RGGWCCY 2 cut(s) 1732, 2274
PstI CTGCAG 3 cut(s) 970, 1228, 1399
PstNI CAGNNNCTG 5 cut(s) 103, 171, 1394, 1471, 2277
PsuI RGATCY 4 cut(s) 581, 1531, 1560, 2209
PvuII CAGCTG 2 cut(s) 1418, 1471
RsaI GTAC 6 cut(s) 1651, 1712, 1751, 2368, 2479, 2616
RsaNI GTAC 6 cut(s) 1650, 1711, 1750, 2367, 2478, 2615
RseI CAYNNNNRTG 1 cut(s) 1881
Sau96I GGNCC 7 cut(s) 174, 437, 550, 748, 1004, 1732, 2274
SbfI CCTGCAGG 1 cut(s) 1399
ScaI AGTACT 1 cut(s) 2368
SchI GAGTC 3 cut(s) 100, 286, 460
ScrFI CCNGG 9 cut(s) 360, 652, 666, 1077, 1239, 1769, 2272, 2466, 2639
SdaI CCTGCAGG 1 cut(s) 1399
SduI GDGCHC 4 cut(s) 75, 876, 1878, 1957
SexAI ACCWGGT 1 cut(s) 1075
SfaNI GCATC 8 cut(s) 292, 1106, 1283, 1433, 1441, 1858, 2054, 2121
SfcI CTRYAG 4 cut(s) 966, 1224, 1395, 1482
SfiI GGCCNNNNNGGCC 1 cut(s) 2267
SfoI GGCGCC 1 cut(s) 2303
Sfr274I CTCGAG 1 cut(s) 2400
SinI GGWCC 4 cut(s) 437, 550, 1732, 2274
SlaI CTCGAG 1 cut(s) 2400
SmiMI CAYNNNNRTG 1 cut(s) 1881
SmlI CTYRAG 4 cut(s) 1956, 2296, 2324, 2400
SmoI CTYRAG 4 cut(s) 1956, 2296, 2324, 2400
Sse8387I CCTGCAGG 1 cut(s) 1399
SsiI CCGC 8 cut(s) 56, 842, 1230, 1351, 1829, 1927, 1999, 2306
SspDI GGCGCC 1 cut(s) 2301
SspI AATATT 1 cut(s) 982
SspMI CTAG 8 cut(s) 267, 522, 1440, 1803, 1931, 1989, 2586, 2644
StyD4I CCNGG 9 cut(s) 358, 650, 664, 1075, 1237, 1767, 2270, 2464, 2637
StyI CCWWGG 2 cut(s) 9, 759
TaaI ACNGT 3 cut(s) 1679, 1785, 2010
TaiI ACGT 3 cut(s) 249, 1594, 2575
TaqI TCGA 5 cut(s) 300, 950, 1595, 2039, 2401
TaqII GACCGA 1 cut(s) 135
TatI WGTACW 5 cut(s) 1649, 1710, 1749, 2366, 2614
TauI GCSGC 3 cut(s) 59, 1232, 1930
TfiI GAWTC 5 cut(s) 604, 700, 1132, 2129, 2144
TscAI CASTG 8 cut(s) 108, 862, 931, 970, 1682, 1903, 2122, 2131
TseFI GTSAC 2 cut(s) 1487, 2194
Tsp45I GTSAC 2 cut(s) 1487, 2194
TspDTI ATGAA 5 cut(s) 1146, 1526, 1693, 2025, 2553
TspRI CASTG 8 cut(s) 108, 862, 931, 970, 1682, 1903, 2122, 2131
Van91I CCANNNNNTGG 2 cut(s) 1022, 1418
VneI GTGCAC 1 cut(s) 1874
VpaK11BI GGWCC 4 cut(s) 437, 550, 1732, 2274
XapI RAATTY 5 cut(s) 460, 640, 991, 1123, 1916
XceI RCATGY 2 cut(s) 718, 1723
XhoI CTCGAG 1 cut(s) 2400
XmiI GTMKAC 1 cut(s) 2575
XmnI GAANNNNTTC 1 cut(s) 2392
XspI CTAG 8 cut(s) 267, 522, 1440, 1803, 1931, 1989, 2586, 2644
ZrmI AGTACT 1 cut(s) 2368
Zsp2I ATGCAT 2 cut(s) 1099, 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.