FvH4_2g08850

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002531
Physical Location & Seq
Reverse (-)
7719065 .. 7725493
6429 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08850.t1

Sequence Viewer

Length: 2631 bp
ATGGTGGATCAGAGAAGAATAGTATTGTTGTTGTGGGTGGTGTTGCTGTTCGAGCCCACATTTCAGCAACCGAGTCAGCAGCCACTGAGTTCACACGCCGACCGAGTTGCTTTGCTTCAGCTCAGATCGTCTTTGGGTCTGAGGAGCAGGGACTGGCCCATTAAGTCAGACCCTTGCTTGGTATGGAGGGGTGTTGGATGCAGTAATGGTAGAGTTGTAGGGATTAACATTTCTGGGTTTAGAAGGACTAGACTCGGGAGTCAAAACCCACAATTTTCGGTTGATGCTTTGGCCAATTTGACCCTTTTGAGCTCTTTCAATGCCTCCAATTTCTTGCTGCCGGGTTCCATTCCCGACTTGTTTGGCCAAAGCCTCGGGTCTCTGCGTGTTCTTGATCTCACTTGGTGTTCGGTGAATGGTCCTATTCCATCGAGTCTTGGAAATTCTACCAATCTGACTAGTCTGTCTTTGTCTCGTAATAACCTTACTGGGACCATTCCTGCTAGTTTGAGCAACCTGGTAAGCCTTTCGGTTCTTGACCTCTCTCAAAATCGGCTTACTGGATCCATTCCCACATCTTTTGGGAATCTCAGGAACCTTTCTGTTCTTGATATTTCTGGGAATTACTTATCCGGGTCACTTCCCTCAGGCATTGGGAACCTCTTGAAGCTTCAGTCTCTGAATCTTTCCAGCAACATGTTGTCTTCTTCCATTCCTCCTGAACTTGGGGGCCTTGATAGCTTGGTTGACCTTGACCTCAGCTCCAATCTATTGTTAGGCCCGCTGCCTTCGGGTTTGAAGAACTTGCAGAGAATTCTAGTTGCAGACAATTTTCTTGGTGGTTCTCTTGGTGTGCTCTGGTCAATGCCTCAATTGAGTTTTATTGATGTGGCTGGTAACAATTTCACTGGTTTGCTGCCCAATTCTAGCTCAAATGCTAATGCCACTGCTGCAGTATTCAATATTTCTCGAAATTCGGTTTATGGGGCTATTACACCATTAGTTGGAAAATTTGGTGTTGTTGATCTTTCAGGTAATTATTTTCAAGGTAAAATACCAGGCTATGTTGGCACCAATGCATCTCTTGACAGCAACTGTCTCCAAAATTTGAAGAATCAGAGGACTTTGGTTGAATGTTCATCGTTTTATGCTGATAGGGGTTTGAGTTTTGATAATTTTGGGCAGCCAAACTCAACACAACCTCCTGCAGCGGCACCGCCTGGGAAGAAGAGTAATAAAAAAGTGATCAAATTGGCATCAGTTTTAGGAGGAGTTGGTTTGATTGTGGTTGTGGTCTTGTTTTTAGTAATACTAGTCCTCTGTTCACGCAAGCGGGGAACTACAACTCAAAGGGGGATTGGTGTGGGGCCAGTTCCTGCAGGCAGCAGTCCCCCACCAGCTGGAGGACCGGTTAACTTTTCTAGTGTAGGAGATGCATTCACATACCAGCAGCTTCTTCAAGCTACGGGTGATTTCAACAGTACAAACTTGATGAAGAATGGCCACACTGGGGATCTCTTCAGGGGTGTCTTGGAAAATGGGATCCCTGTGGTTGTTAAAAGGATTGATTTACGTTCGATTAAGAAGGATGCGTACTTGCTGGAGTTGGATTTCTTCAGTAAAGTGACAAGTACAAGATTTGTTCCCTTTCTGGGACACTGTCTAGAGAATGAGAATGAGAAGTTCATGGTGTACAAATACATGCCAAATGGGGACCTATCAAGTTCTTTGTACAAGAGTACCAACACCCTGGATGATACTTCGTTACAGTCATTGGATTGGATCACTAGATTAAAGATTGCATTAGGTGCTGCAGAGGGTCTAACTTATCTGCATCATGAGTGTGATCCACCCCTCGTGCACAGAGATGTTCAAGCAAGCAGTATACTTCTTGATGATAAATTTGAAGTGCGGCTAGGAAGCCTGAGTGACGTCTGCGCTCAAGAAGGAGATACTCATCACAGCAGGATTACTAGGTTGCTGCGTTTGCCACAGTCATCCGAACAAGGTGCTTCTGGTTCATCAACAGCATCATGTGCCTATGACGTCTATTGTTTAGGGAAGGTGTTACTTGAACTGGTAACAGGTAAATTGGGCATCAGTGCTGCCAGTGAATCCCAATTGAAGGAATCGTTGGATGACATACTTCCCAACATCAGTATATATGACAAGGAACTTCTGAACAAAATTGTGGATCCGTCTTTGATTGTTGATGAGGACCTATTGGAGGAAGTGTGGGCCATGGCTGTTGTGGCCAGGTCCTGCCTAAATCCCAAGCCCTCAAGGCGTCCACTAATGAGATATATTCTCAAGGCTTTGGAAAACCCGGTCAAGGTCGTAAGGGAAGATAGTACTGGCTCTGCAAGGCTCAAAACCGCCTCTTCTCGAGGGTCATGGAATGCTGCTGTATTTGGTAGCTGGCGCAGCTCATCTGAGGTAGTGGTTATTCCAGGAGCGTCAGGTACCAAAGTGGAAGGAGCAAGTGGTTTGAAGCATTCAGCAACCACCGGTTCGCAGGGAAGTGGCCAAAATGGTGGGGGTGAACATTCATCCTCACGTAGACGGCATTCTAGGGATATTTTTCCCGAACCATCTGGTGTACAAGATGTAGAGAGATATGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

877

Amino Acids

93.86

Weight (kDa)

8.46

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 124 - 255 1.6e-15 Leucine-rich repeat region
LRR_8 PF13855 150 - 210 5.7e-11 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 498 - 712 2.5e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 500 - 770 4.9e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 258, 463
AatII GACGTC 2 cut(s) 1935, 2049
Acc65I GGTACC 1 cut(s) 2462
AccB1I GGYRCC 3 cut(s) 1070, 1213, 2462
AccB7I CCANNNNNTGG 2 cut(s) 1004, 1400
AccI GTMKAC 2 cut(s) 1885, 2560
AciI CCGC 6 cut(s) 782, 1211, 1217, 1333, 1912, 2376
AcoI YGGCCR 5 cut(s) 291, 364, 1501, 2253, 2524
AcsI RAATTY 6 cut(s) 442, 813, 973, 1010, 1105, 1901
AcuI CTGAAG 4 cut(s) 101, 656, 1504, 1600
AcyI GRCGYC 3 cut(s) 1932, 2046, 2287
AdeI CACNNNGTG 1 cut(s) 405
AfaI GTAC 9 cut(s) 1483, 1595, 1633, 1694, 1733, 1741, 2353, 2464, 2601
AflIII ACRYGT 1 cut(s) 696
AgeI ACCGGT 2 cut(s) 1408, 2507
AhlI ACTAGT 2 cut(s) 458, 1312
AjnI CCWGG 7 cut(s) 516, 1057, 1219, 1749, 2255, 2449, 2622
AjuI GAANNNNNNNTTGG 2 cut(s) 2117, 2149
Alw21I GWGCWC 3 cut(s) 314, 858, 1863
Alw26I GTCTC 4 cut(s) 384, 477, 681, 1103
Alw44I GTGCAC 1 cut(s) 1859
AlwNI CAGNNNCTG 6 cut(s) 85, 153, 679, 1095, 1376, 2262
Ama87I CYCGRG 3 cut(s) 254, 374, 2385
ApaLI GTGCAC 1 cut(s) 1859
ApoI RAATTY 6 cut(s) 442, 813, 973, 1010, 1105, 1901
AsiGI ACCGGT 2 cut(s) 1408, 2507
Asp718I GGTACC 1 cut(s) 2462
AspLEI GCGC 2 cut(s) 1940, 2424
AsuC2I CCSGG 3 cut(s) 342, 634, 2327
AsuHPI GGTGA 3 cut(s) 424, 1481, 2552
AvaI CYCGRG 3 cut(s) 254, 374, 2385
AvaII GGWCC 6 cut(s) 419, 492, 1406, 1714, 2218, 2259
AxyI CCTNAGG 1 cut(s) 646
BaeGI GKGCMC 1 cut(s) 1863
BaeI ACNNNNGTAYC 4 cut(s) 1723, 1756, 2454, 2487
BalI TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
BamHI GGATCC 3 cut(s) 563, 1542, 2194
BanI GGYRCC 3 cut(s) 1070, 1213, 2462
BanII GRGCYC 2 cut(s) 57, 314
BarI GAAGNNNNNNTAC 2 cut(s) 1577, 1609
BauI CACGAG 1 cut(s) 1856
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 3 cut(s) 314, 858, 1863
BbvCI CCTCAGC 1 cut(s) 758
BccI CCATC 2 cut(s) 436, 2599
BceAI ACGGC 1 cut(s) 2579
BcgI CGANNNNNNTGC 4 cut(s) 89, 123, 1991, 2025
BciT130I CCWGG 7 cut(s) 518, 1059, 1221, 1751, 2257, 2451, 2624
BclI TGATCA 1 cut(s) 1245
BcnI CCSGG 3 cut(s) 342, 634, 2327
BcoDI GTCTC 4 cut(s) 384, 477, 681, 1103
BcuI ACTAGT 2 cut(s) 458, 1312
BfmI CTRYAG 4 cut(s) 951, 1206, 1377, 1812
BglI GCCNNNNNGGC 1 cut(s) 2284
BmcAI AGTACT 1 cut(s) 2353
Bme18I GGWCC 6 cut(s) 419, 492, 1406, 1714, 2218, 2259
BmeT110I CYCGRG 3 cut(s) 254, 374, 2385
BmrI ACTGGG 2 cut(s) 498, 1518
BmsI GCATC 9 cut(s) 188, 274, 1088, 1265, 1423, 1579, 1843, 2039, 2106
BmuI ACTGGG 2 cut(s) 498, 1518
BpiI GAAGAC 1 cut(s) 696
BpmI CTGGAG 2 cut(s) 1422, 1622
Bpu10I CCTNAGC 1 cut(s) 758
BpuEI CTTGAG 3 cut(s) 1926, 2266, 2294
BpuMI CCSGG 3 cut(s) 342, 634, 2327
BsaAI YACGTR 1 cut(s) 2558
BsaBI GATNNNNATC 1 cut(s) 1956
BsaHI GRCGYC 3 cut(s) 1932, 2046, 2287
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 4 cut(s) 373, 1220, 1749, 2241
BsaWI WCCGGW 2 cut(s) 1408, 2507
BsaXI ACNNNNNCTCC 8 cut(s) 746, 776, 1186, 1216, 1395, 1425, 2219, 2249
Bse118I RCCGGY 2 cut(s) 1408, 2507
Bse1I ACTGG 9 cut(s) 158, 493, 565, 913, 1370, 1513, 2082, 2109, 2359
Bse21I CCTNAGG 1 cut(s) 646
Bse8I GATNNNNATC 1 cut(s) 1956
BseBI CCWGG 7 cut(s) 518, 1059, 1221, 1751, 2257, 2451, 2624
BseDI CCNNGG 4 cut(s) 373, 1220, 1749, 2241
BseGI GGATG 6 cut(s) 203, 1594, 1759, 1997, 2143, 2549
BseJI GATNNNNATC 1 cut(s) 1956
BseMII CTCAG 8 cut(s) 77, 131, 136, 604, 660, 772, 1916, 2424
BseNI ACTGG 9 cut(s) 158, 493, 565, 913, 1370, 1513, 2082, 2109, 2359
BseRI GAGGAG 2 cut(s) 157, 1284
BseSI GKGCMC 1 cut(s) 1863
Bsh1285I CGRYCG 1 cut(s) 103
BshNI GGYRCC 3 cut(s) 1070, 1213, 2462
BshTI ACCGGT 2 cut(s) 1408, 2507
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 3 cut(s) 314, 858, 1863
BsiHKCI CYCGRG 3 cut(s) 254, 374, 2385
BsiSI CCGG 5 cut(s) 341, 633, 1409, 2327, 2508
BslFI GGGAC 5 cut(s) 164, 505, 1374, 1668, 1727
BsmAI GTCTC 4 cut(s) 384, 477, 681, 1103
BsmFI GGGAC 5 cut(s) 164, 505, 1374, 1668, 1727
BsmI GAATGC 4 cut(s) 1436, 2404, 2494, 2566
Bso31I GGTCTC 1 cut(s) 384
BsoBI CYCGRG 3 cut(s) 254, 374, 2385
Bsp1286I GDGCHC 4 cut(s) 57, 314, 858, 1863
Bsp1407I TGTACA 3 cut(s) 1692, 1731, 2599
Bsp19I CCATGG 1 cut(s) 2241
BspACI CCGC 6 cut(s) 782, 1211, 1217, 1333, 1912, 2376
BspCNI CTCAG 8 cut(s) 78, 132, 135, 603, 659, 771, 1917, 2425
BspHI TCATGA 1 cut(s) 1837
BspMAI CTGCAG 4 cut(s) 955, 1210, 1381, 1816
BspT107I GGYRCC 3 cut(s) 1070, 1213, 2462
BspTNI GGTCTC 1 cut(s) 384
BsrFI RCCGGY 2 cut(s) 1408, 2507
BsrGI TGTACA 3 cut(s) 1692, 1731, 2599
BsrI ACTGG 9 cut(s) 158, 493, 565, 913, 1370, 1513, 2082, 2109, 2359
BssAI RCCGGY 2 cut(s) 1408, 2507
BssECI CCNNGG 4 cut(s) 373, 1220, 1749, 2241
BssNAI GTATAC 1 cut(s) 1886
BssNI GRCGYC 3 cut(s) 1932, 2046, 2287
BssSI CACGAG 1 cut(s) 1856
BssT1I CCWWGG 1 cut(s) 2241
Bst1107I GTATAC 1 cut(s) 1886
Bst2BI CACGAG 1 cut(s) 1856
Bst2UI CCWGG 7 cut(s) 518, 1059, 1221, 1751, 2257, 2451, 2624
Bst4CI ACNGT 5 cut(s) 1097, 1481, 1661, 1770, 1995
Bst6I CTCTTC 3 cut(s) 1223, 1523, 2386
BstACI GRCGYC 3 cut(s) 1932, 2046, 2287
BstAPI GCANNNNNTGC 2 cut(s) 1808, 2036
BstAUI TGTACA 3 cut(s) 1692, 1731, 2599
BstBAI YACGTR 1 cut(s) 2558
BstC8I GCNNGC 5 cut(s) 782, 1331, 1381, 1879, 2420
BstDEI CTNAG 8 cut(s) 86, 122, 140, 590, 646, 758, 1925, 2433
BstDSI CCRYGG 1 cut(s) 2241
BstENI CCTNNNNNAGG 1 cut(s) 2225
BstF5I GGATG 6 cut(s) 203, 1594, 1759, 1997, 2143, 2549
BstHHI GCGC 2 cut(s) 1940, 2424
BstMAI GTCTC 4 cut(s) 384, 477, 681, 1103
BstMCI CGRYCG 1 cut(s) 103
BstNI CCWGG 7 cut(s) 518, 1059, 1221, 1751, 2257, 2451, 2624
BstNSI RCATGY 2 cut(s) 700, 1705
BstSFI CTRYAG 4 cut(s) 951, 1206, 1377, 1812
BstSLI GKGCMC 1 cut(s) 1863
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 4 cut(s) 563, 1513, 1542, 2194
BstXI CCANNNNNNTGG 2 cut(s) 1750, 2534
BstYI RGATCY 4 cut(s) 563, 1513, 1542, 2194
BstZ17I GTATAC 1 cut(s) 1886
Bsu36I CCTNAGG 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 2241
BtsCI GGATG 6 cut(s) 203, 1594, 1759, 1997, 2143, 2549
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 7 cut(s) 83, 906, 945, 1506, 1657, 2107, 2116
Cac8I GCNNGC 5 cut(s) 782, 1331, 1381, 1879, 2420
CaiI CAGNNNCTG 6 cut(s) 85, 153, 679, 1095, 1376, 2262
CciI TCATGA 1 cut(s) 1837
CfoI GCGC 2 cut(s) 1940, 2424
Cfr10I RCCGGY 2 cut(s) 1408, 2507
CseI GACGC 2 cut(s) 2276, 2445
CsiI ACCWGGT 1 cut(s) 516
Csp6I GTAC 9 cut(s) 1482, 1594, 1632, 1693, 1732, 1740, 2352, 2463, 2600
CspAI ACCGGT 2 cut(s) 1408, 2507
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 7 cut(s) 697, 1687, 1702, 1838, 2034, 2242, 2394
CviQI GTAC 9 cut(s) 1482, 1594, 1632, 1693, 1732, 1740, 2352, 2463, 2600
DdeI CTNAG 8 cut(s) 86, 122, 140, 590, 646, 758, 1925, 2433
DraIII CACNNNGTG 1 cut(s) 405
DrdI GACNNNNNNGTC 2 cut(s) 258, 463
DseDI GACNNNNNNGTC 2 cut(s) 258, 463
EaeI YGGCCR 5 cut(s) 291, 364, 1501, 2253, 2524
Eam1104I CTCTTC 3 cut(s) 1223, 1523, 2386
EarI CTCTTC 3 cut(s) 1223, 1523, 2386
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 2241
Eco24I GRGCYC 2 cut(s) 57, 314
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 6 cut(s) 419, 492, 1406, 1714, 2218, 2259
Eco53kI GAGCTC 1 cut(s) 312
Eco57I CTGAAG 4 cut(s) 101, 656, 1504, 1600
Eco81I CCTNAGG 1 cut(s) 646
Eco88I CYCGRG 3 cut(s) 254, 374, 2385
EcoICRI GAGCTC 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 2225
EcoO109I RGGNCCY 4 cut(s) 730, 1714, 2218, 2259
EcoRI GAATTC 1 cut(s) 813
EcoRII CCWGG 7 cut(s) 516, 1057, 1219, 1749, 2255, 2449, 2622
EcoT14I CCWWGG 1 cut(s) 2241
EcoT22I ATGCAT 2 cut(s) 1081, 1438
EcoT38I GRGCYC 2 cut(s) 57, 314
ErhI CCWWGG 1 cut(s) 2241
FaeI CATG 7 cut(s) 700, 1690, 1705, 1841, 2037, 2245, 2397
FaqI GGGAC 5 cut(s) 164, 505, 1374, 1668, 1727
FatI CATG 7 cut(s) 696, 1686, 1701, 1837, 2033, 2241, 2393
FauI CCCGC 2 cut(s) 789, 1326
FbaI TGATCA 1 cut(s) 1245
FblI GTMKAC 2 cut(s) 1885, 2560
FokI GGATG 6 cut(s) 210, 1601, 1766, 1984, 2150, 2536
FriOI GRGCYC 2 cut(s) 57, 314
GlaI GCGC 2 cut(s) 1939, 2423
GsuI CTGGAG 2 cut(s) 1422, 1622
HapII CCGG 5 cut(s) 341, 633, 1409, 2327, 2508
HgaI GACGC 2 cut(s) 2276, 2445
HhaI GCGC 2 cut(s) 1940, 2424
Hin1I GRCGYC 3 cut(s) 1932, 2046, 2287
Hin1II CATG 7 cut(s) 700, 1690, 1705, 1841, 2037, 2245, 2397
Hin6I GCGC 2 cut(s) 1938, 2422
HinP1I GCGC 2 cut(s) 1938, 2422
HincII GTYRAC 2 cut(s) 748, 1414
HindII GTYRAC 2 cut(s) 748, 1414
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 9 cut(s) 73, 252, 259, 433, 586, 682, 1114, 2114, 2129
HpaI GTTAAC 1 cut(s) 1414
HpaII CCGG 5 cut(s) 341, 633, 1409, 2327, 2508
HphI GGTGA 3 cut(s) 424, 1481, 2552
HpyAV CCTTC 7 cut(s) 237, 798, 1579, 1940, 2056, 2119, 2468
HpyCH4III ACNGT 5 cut(s) 1097, 1481, 1661, 1770, 1995
HpyCH4IV ACGT 4 cut(s) 1573, 1932, 2046, 2557
HpyF3I CTNAG 8 cut(s) 86, 122, 140, 590, 646, 758, 1925, 2433
HpySE526I ACGT 4 cut(s) 1573, 1932, 2046, 2557
Hsp92I GRCGYC 3 cut(s) 1932, 2046, 2287
Hsp92II CATG 7 cut(s) 700, 1690, 1705, 1841, 2037, 2245, 2397
HspAI GCGC 2 cut(s) 1938, 2422
KpnI GGTACC 1 cut(s) 2466
Ksp22I TGATCA 1 cut(s) 1245
KspAI GTTAAC 1 cut(s) 1414
LmnI GCTCC 4 cut(s) 144, 767, 2453, 2477
LweI GCATC 9 cut(s) 188, 274, 1088, 1265, 1423, 1579, 1843, 2039, 2106
MabI ACCWGGT 1 cut(s) 516
MaeII ACGT 4 cut(s) 1573, 1932, 2046, 2557
MaeIII GTNAC 7 cut(s) 636, 896, 1624, 1764, 1928, 2067, 2080
MfeI CAATTG 2 cut(s) 872, 2120
MflI RGATCY 4 cut(s) 563, 1513, 1542, 2194
MhlI GDGCHC 4 cut(s) 57, 314, 858, 1863
MlsI TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
MluNI TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
MlyI GAGTC 4 cut(s) 82, 246, 268, 442
MmeI TCCRAC 4 cut(s) 175, 985, 1587, 2115
Mox20I TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
Mph1103I ATGCAT 2 cut(s) 1081, 1438
MscI TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
MseI TTAA 6 cut(s) 162, 225, 1413, 1557, 1581, 1793
MslI CAYNNNNRTG 2 cut(s) 1842, 1866
Msp20I TGGCCA 5 cut(s) 293, 366, 1503, 2255, 2526
MspA1I CMGCKG 3 cut(s) 784, 1211, 1400
MspI CCGG 5 cut(s) 341, 633, 1409, 2327, 2508
MunI CAATTG 2 cut(s) 872, 2120
Mva1269I GAATGC 4 cut(s) 1436, 2404, 2494, 2566
MvaI CCWGG 7 cut(s) 518, 1059, 1221, 1751, 2257, 2451, 2624
NciI CCSGG 3 cut(s) 342, 634, 2327
NcoI CCATGG 1 cut(s) 2241
NlaIII CATG 7 cut(s) 700, 1690, 1705, 1841, 2037, 2245, 2397
NmuCI GTSAC 3 cut(s) 636, 1624, 1928
NsiI ATGCAT 2 cut(s) 1081, 1438
NspI RCATGY 2 cut(s) 700, 1705
PaeR7I CTCGAG 1 cut(s) 2385
PagI TCATGA 1 cut(s) 1837
PciI ACATGT 1 cut(s) 696
PctI GAATGC 4 cut(s) 1436, 2404, 2494, 2566
PfeI GAWTC 5 cut(s) 586, 682, 1114, 2114, 2129
PflFI GACNNNGTC 1 cut(s) 1659
PflMI CCANNNNNTGG 2 cut(s) 1004, 1400
PfoI TCCNGGA 1 cut(s) 2449
PinAI ACCGGT 2 cut(s) 1408, 2507
PleI GAGTC 4 cut(s) 81, 246, 267, 441
PpsI GAGTC 4 cut(s) 81, 246, 267, 441
Ppu21I YACGTR 1 cut(s) 2558
PpuMI RGGWCCY 3 cut(s) 1714, 2218, 2259
PscI ACATGT 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 314
Psp5II RGGWCCY 3 cut(s) 1714, 2218, 2259
Psp6I CCWGG 7 cut(s) 516, 1057, 1219, 1749, 2255, 2449, 2622
PspGI CCWGG 7 cut(s) 516, 1057, 1219, 1749, 2255, 2449, 2622
PspPPI RGGWCCY 3 cut(s) 1714, 2218, 2259
PstI CTGCAG 4 cut(s) 955, 1210, 1381, 1816
PstNI CAGNNNCTG 6 cut(s) 85, 153, 679, 1095, 1376, 2262
PsuI RGATCY 4 cut(s) 563, 1513, 1542, 2194
PsyI GACNNNGTC 1 cut(s) 1659
PvuII CAGCTG 1 cut(s) 1400
RsaI GTAC 9 cut(s) 1483, 1595, 1633, 1694, 1733, 1741, 2353, 2464, 2601
RsaNI GTAC 9 cut(s) 1482, 1594, 1632, 1693, 1732, 1740, 2352, 2463, 2600
RseI CAYNNNNRTG 2 cut(s) 1842, 1866
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 6 cut(s) 162, 225, 1413, 1557, 1581, 1793
SbfI CCTGCAGG 1 cut(s) 1381
ScaI AGTACT 1 cut(s) 2353
SchI GAGTC 4 cut(s) 82, 246, 268, 442
SdaI CCTGCAGG 1 cut(s) 1381
SduI GDGCHC 4 cut(s) 57, 314, 858, 1863
SexAI ACCWGGT 1 cut(s) 516
SfaNI GCATC 9 cut(s) 188, 274, 1088, 1265, 1423, 1579, 1843, 2039, 2106
SfcI CTRYAG 4 cut(s) 951, 1206, 1377, 1812
Sfr274I CTCGAG 1 cut(s) 2385
SinI GGWCC 6 cut(s) 419, 492, 1406, 1714, 2218, 2259
SlaI CTCGAG 1 cut(s) 2385
SmiMI CAYNNNNRTG 2 cut(s) 1842, 1866
SmlI CTYRAG 4 cut(s) 1941, 2281, 2309, 2385
SmoI CTYRAG 4 cut(s) 1941, 2281, 2309, 2385
SpeI ACTAGT 2 cut(s) 458, 1312
Sse8387I CCTGCAGG 1 cut(s) 1381
SsiI CCGC 6 cut(s) 782, 1211, 1217, 1333, 1912, 2376
SspI AATATT 1 cut(s) 964
SstI GAGCTC 1 cut(s) 314
StyI CCWWGG 1 cut(s) 2241
TaaI ACNGT 5 cut(s) 1097, 1481, 1661, 1770, 1995
TaiI ACGT 4 cut(s) 1576, 1935, 2049, 2560
TaqI TCGA 5 cut(s) 51, 431, 970, 1577, 2386
TaqII GACCGA 1 cut(s) 117
TatI WGTACW 6 cut(s) 1481, 1631, 1692, 1731, 2351, 2599
TauI GCSGC 2 cut(s) 1214, 1915
TfiI GAWTC 5 cut(s) 586, 682, 1114, 2114, 2129
Tru1I TTAA 6 cut(s) 162, 225, 1413, 1557, 1581, 1793
Tru9I TTAA 6 cut(s) 162, 225, 1413, 1557, 1581, 1793
TscAI CASTG 7 cut(s) 90, 913, 952, 1513, 1664, 2107, 2116
TseFI GTSAC 3 cut(s) 636, 1624, 1928
Tsp45I GTSAC 3 cut(s) 636, 1624, 1928
TspDTI ATGAA 5 cut(s) 1128, 1508, 1675, 2010, 2538
TspGWI ACGGA 1 cut(s) 2187
TspRI CASTG 7 cut(s) 90, 913, 952, 1513, 1664, 2107, 2116
Tth111I GACNNNGTC 1 cut(s) 1659
Van91I CCANNNNNTGG 2 cut(s) 1004, 1400
VneI GTGCAC 1 cut(s) 1859
VpaK11BI GGWCC 6 cut(s) 419, 492, 1406, 1714, 2218, 2259
XagI CCTNNNNNAGG 1 cut(s) 2225
XapI RAATTY 6 cut(s) 442, 813, 973, 1010, 1105, 1901
XbaI TCTAGA 1 cut(s) 1663
XceI RCATGY 2 cut(s) 700, 1705
XcmI CCANNNNNNNNNTGG 1 cut(s) 2248
XhoI CTCGAG 1 cut(s) 2385
XmiI GTMKAC 2 cut(s) 1885, 2560
ZraI GACGTC 2 cut(s) 1933, 2047
ZrmI AGTACT 1 cut(s) 2353
Zsp2I ATGCAT 2 cut(s) 1081, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.