pycom08g02470

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002531
Physical Location & Seq
Forward (+)
1819600 .. 1821213
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02470.1

Sequence Viewer

Length: 1614 bp
ATGAAGTTGTTATCTGTAACTAAAACGGCAATGGGGGCGAGAGCCCAAGTCGGCGCCTTTGCCATAGCGTTAATCTTGTTCGTGATTCAGAGTGGTTTTGCGCAGCAGGGTTCTTTGAGCTCCGAAATGGAGCTGTCGGCGTTACTGGAGCTCCGGTCTTCGTTGGGTCTCAGAAGCAAAAACTGGCCCAAAAGGGCCGACCCATGTTCATCCTGGACCGGAGTCACATGCAGCAAGAACGGCCGAGTCACTGGAATTACCTTGTCCGGTTTGAGGCGAACCCGAGTGGGCCGGCAAAACCCCCGGTTCTCCGTCGATTCACTGGCTAATATAACCCTCTTGGCTTCTTTCAACGCCTCCGGGTTCGCGCTTCCTGGGTCCATACCCGACTGGTTTGGCCAAAGACTTGGTGCACTCCAGGTGCTCGACCTTAGGTCTGCGTCAGTCATTGGTTCTATTCCCCAGTCGCTTGGTAATTTGAAAAATCTTACTTCTTTGTTTTTATCTGGCAATGATATTACTGGGATTTTACCTTCTGCGTTGATAAACATAACAGAGTTGGAAATTCTTGATCTTTCGAGGAATGCGATTACTGGGTCCATACCTTCTGGCTTTGCCAATCTTGGAAAGCTTGAAAGTCTTGACCTTTCTTCAAATTTCTTATATGGGTCGGTTCCACCAGGTTTGGGAACCCTTTCAAGGCTTGAGTTCTTGGACTTGTCTGATAATAGTCTCACCGATTCGATTCCGGTTCAGTTTAGTAACCTTTCCCGGTTGGTTGAGCTTGATCTTAGCAAGAACACTTTGTCTGGGGCATTGCCTGTGGAGCTGAGAGGGTTGAGGAGTTTGATGAGGATGAGGATTGGAGATAATGCTCTCGAAGGTCCATTGCCGGAGGGTTTGTTTTCGAGTCTTGTTCAGCTGGAAGTTCTGGTTCTGAGTCGGAATAAATTTGATGGTGCTCTTCCTGGTGCCTTGTGGTCACTTCCCAGTTTGCGATTTCTCGATGTATCCCGCAACAATTTTACGGGAACTCTACCGAGCCTGGGTTCAAATGCTAGTGTTAGTGGCGCTGTGTTCAACCTTTCTCATAATCTGTTATATGGAAATCTTACTTTTCCTGTTGGGAAATTTGTCTCAATCGATTTGTCTGATAATTTTTTCCAAGGCAAGGTATTAGAAGATAGTCAAAGCAATGATACTCTTACTGGAAATTGCCTACAGATTGTACCAAATCAAAGGAGTTTGCAGGATTGTAGGCAGTTTTATGAAGAGAGAAATTTAATTTTTGATGATTTTGGGGCCCTAGAACCTGCTCAGCCACCTTTCCTAGAACCTGAATCAAAGAGCAAGAACAGATTAACATTTATATTGGTTGGGATATTTGGTGGACTCGGCTTCATTGTGATTTTGGTACTGGTTCTGGTAGTGCTCCTGAAAATGTGCAATAAAGGCACAAATCAACGTGGAAGTGCAAATGTTGGGCCTGTTCCAGAAGGAGATGACCCTGCACTCCCCAAAGATCGATCATTGATGCATCAGGTCGAGGAGATTCATTTACGTATGAGCAGATTCTCCAGTCCACTTCTGCTTTCAGTGAAGCAAATCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

538

Amino Acids

57.95

Weight (kDa)

8.24

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 43 - 78 1.8e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 136 - 217 5.1e-07 Leucine-rich repeat region
LRR_8 PF13855 162 - 219 5.8e-11 Leucine rich repeat
LRR_14 PF23598 229 - 338 1.5e-07 Leucine-rich repeat region
LRR_8 PF13855 234 - 293 1e-07 Leucine rich repeat
LRR_4 PF12799 234 - 270 9.5e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 281 - 342 4.9e-10 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1612
Acc16I TGCGCA 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 1321
AccB1I GGYRCC 2 cut(s) 53, 971
AccII CGCG 1 cut(s) 368
AciI CCGC 1 cut(s) 1015
AcoI YGGCCR 2 cut(s) 241, 397
AcsI RAATTY 5 cut(s) 564, 655, 950, 1130, 1279
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 1230, 1416
AfiI CCNNNNNNNGG 5 cut(s) 273, 686, 699, 1046, 1171
AgsI TTSAA 7 cut(s) 352, 481, 635, 654, 699, 1053, 1081
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 6 cut(s) 212, 373, 417, 679, 967, 1044
AluBI AGCT 7 cut(s) 120, 133, 151, 631, 784, 829, 922
AluI AGCT 7 cut(s) 120, 133, 151, 631, 784, 829, 922
Alw21I GWGCWC 6 cut(s) 122, 153, 415, 426, 964, 1434
Alw26I GTCTC 3 cut(s) 173, 737, 1141
Alw44I GTGCAC 1 cut(s) 411
Ama87I CYCGRG 1 cut(s) 282
AoxI GGCC 7 cut(s) 185, 195, 241, 289, 397, 1302, 1484
ApaI GGGCCC 1 cut(s) 1306
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 103, 231
ApoI RAATTY 5 cut(s) 564, 655, 950, 1130, 1279
Asp700I GAANNNNTTC 1 cut(s) 694
AspLEI GCGC 4 cut(s) 56, 103, 370, 1073
AsuC2I CCSGG 3 cut(s) 304, 361, 772
AsuHPI GGTGA 1 cut(s) 727
AvaI CYCGRG 1 cut(s) 282
AvaII GGWCC 4 cut(s) 216, 378, 597, 884
AxyI CCTNAGG 1 cut(s) 431
BaeGI GKGCMC 2 cut(s) 415, 1306
BalI TGGCCA 1 cut(s) 399
BanI GGYRCC 2 cut(s) 53, 971
BanII GRGCYC 4 cut(s) 46, 122, 153, 1306
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 6 cut(s) 122, 153, 415, 426, 964, 1434
BbvI GCAGC 2 cut(s) 115, 243
BccI CCATC 1 cut(s) 950
BceAI ACGGC 2 cut(s) 42, 256
BciT130I CCWGG 6 cut(s) 214, 375, 419, 681, 969, 1046
BciVI GTATCC 1 cut(s) 1021
BcnI CCSGG 3 cut(s) 304, 361, 772
BcoDI GTCTC 3 cut(s) 173, 737, 1141
BfaI CTAG 3 cut(s) 1059, 1307, 1331
BfmI CTRYAG 1 cut(s) 1220
BfoI RGCGCY 2 cut(s) 57, 1074
BfuAI ACCTGC 1 cut(s) 1321
BfuI GTATCC 1 cut(s) 1021
BisI GCNGC 2 cut(s) 104, 232
BlpI GCTNAGC 1 cut(s) 1317
BlsI GCNGC 2 cut(s) 105, 233
Bme1390I CCNGG 9 cut(s) 214, 304, 361, 375, 419, 681, 772, 969, 1046
Bme18I GGWCC 4 cut(s) 216, 378, 597, 884
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 282
BmiI GGNNCC 8 cut(s) 55, 379, 598, 675, 691, 973, 1303, 1304
BmrFI CCNGG 9 cut(s) 214, 304, 361, 375, 419, 681, 772, 969, 1046
BmrI ACTGGG 4 cut(s) 457, 531, 603, 984
BmsI GCATC 2 cut(s) 1524, 1546
BmuI ACTGGG 4 cut(s) 457, 531, 603, 984
BoxI GACNNNNGTC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 3 cut(s) 167, 401, 1561
Bpu1102I GCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 3 cut(s) 304, 361, 772
Bsa29I ATCGAT 2 cut(s) 1143, 1525
BsaAI YACGTR 1 cut(s) 1562
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 4 cut(s) 302, 374, 1045, 1165
BsaWI WCCGGW 4 cut(s) 153, 218, 266, 748
BsaXI ACNNNNNCTCC 6 cut(s) 135, 140, 165, 170, 887, 917
Bsc4I CCNNNNNNNGG 5 cut(s) 273, 686, 699, 1046, 1171
Bse118I RCCGGY 1 cut(s) 291
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
BseBI CCWGG 6 cut(s) 214, 375, 419, 681, 969, 1046
BseCI ATCGAT 2 cut(s) 1143, 1525
BseDI CCNNGG 4 cut(s) 302, 374, 1045, 1165
BseGI GGATG 2 cut(s) 209, 861
BseLI CCNNNNNNNGG 5 cut(s) 273, 686, 699, 1046, 1171
BseMI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
BseMII CTCAG 4 cut(s) 184, 821, 929, 1331
BseRI GAGGAG 2 cut(s) 856, 1562
BseSI GKGCMC 2 cut(s) 415, 1306
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 115, 243
BsgI GTGCAG 1 cut(s) 1494
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 7 cut(s) 187, 197, 243, 291, 399, 1304, 1486
BshNI GGYRCC 2 cut(s) 53, 971
BshVI ATCGAT 2 cut(s) 1143, 1525
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 6 cut(s) 122, 153, 415, 426, 964, 1434
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 9 cut(s) 154, 219, 267, 292, 304, 360, 749, 772, 893
BslI CCNNNNNNNGG 5 cut(s) 273, 686, 699, 1046, 1171
BsmAI GTCTC 3 cut(s) 173, 737, 1141
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 7 cut(s) 187, 197, 243, 291, 399, 1304, 1486
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 282
Bsp120I GGGCCC 1 cut(s) 1302
Bsp1286I GDGCHC 8 cut(s) 46, 122, 153, 415, 426, 964, 1306, 1434
Bsp143I GATC 4 cut(s) 571, 787, 1522, 1526
Bsp1720I GCTNAGC 1 cut(s) 1317
BspACI CCGC 1 cut(s) 1015
BspANI GGCC 7 cut(s) 187, 197, 243, 291, 399, 1304, 1486
BspCNI CTCAG 4 cut(s) 183, 822, 930, 1330
BspDI ATCGAT 2 cut(s) 1143, 1525
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 8 cut(s) 55, 379, 598, 675, 691, 973, 1303, 1304
BspMI ACCTGC 1 cut(s) 1321
BspQI GCTCTTC 1 cut(s) 969
BspT107I GGYRCC 2 cut(s) 53, 971
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 5 cut(s) 36, 517, 815, 887, 1201
BsrFI RCCGGY 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 4 cut(s) 302, 374, 1045, 1165
BssMI GATC 4 cut(s) 571, 787, 1522, 1526
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 1165
Bst2UI CCWGG 6 cut(s) 214, 375, 419, 681, 969, 1046
Bst6I CTCTTC 2 cut(s) 969, 1266
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 1562
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 6 cut(s) 170, 431, 791, 830, 938, 1317
BstF5I GGATG 2 cut(s) 209, 861
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 2 cut(s) 57, 1074
BstHHI GCGC 4 cut(s) 56, 103, 370, 1073
BstKTI GATC 4 cut(s) 574, 790, 1525, 1529
BstMAI GTCTC 3 cut(s) 173, 737, 1141
BstMBI GATC 4 cut(s) 571, 787, 1522, 1526
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 4 cut(s) 35, 240, 826, 1452
BstNI CCWGG 6 cut(s) 214, 375, 419, 681, 969, 1046
BstNSI RCATGY 1 cut(s) 231
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 9 cut(s) 212, 302, 359, 373, 417, 679, 770, 967, 1044
BstSFI CTRYAG 1 cut(s) 1220
BstSLI GKGCMC 2 cut(s) 415, 1306
BstSNI TACGTA 1 cut(s) 1562
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 2 cut(s) 115, 243
BstV2I GAAGAC 1 cut(s) 150
BstXI CCANNNNNNTGG 2 cut(s) 407, 470
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 2 cut(s) 1143, 1525
Bsu36I CCTNAGG 1 cut(s) 431
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 7 cut(s) 187, 197, 243, 291, 399, 1304, 1486
BsuTUI ATCGAT 2 cut(s) 1143, 1525
BtsCI GGATG 2 cut(s) 209, 861
BtsIMutI CAGTG 3 cut(s) 249, 320, 1602
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 1 cut(s) 293
CfoI GCGC 4 cut(s) 56, 103, 370, 1073
Cfr10I RCCGGY 1 cut(s) 291
ClaI ATCGAT 2 cut(s) 1143, 1525
CseI GACGC 1 cut(s) 429
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 2 cut(s) 1229, 1415
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviAII CATG 2 cut(s) 204, 228
CviQI GTAC 2 cut(s) 1229, 1415
DdeI CTNAG 6 cut(s) 170, 431, 791, 830, 938, 1317
DinI GGCGCC 1 cut(s) 55
DpnI GATC 4 cut(s) 573, 789, 1524, 1528
DpnII GATC 4 cut(s) 571, 787, 1522, 1526
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 241, 397
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 969, 1266
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 2 cut(s) 969, 1266
Ecl136II GAGCTC 2 cut(s) 120, 151
EclXI CGGCCG 1 cut(s) 241
Eco105I TACGTA 1 cut(s) 1562
Eco130I CCWWGG 1 cut(s) 1165
Eco24I GRGCYC 4 cut(s) 46, 122, 153, 1306
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 4 cut(s) 216, 378, 597, 884
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 120, 151
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 282
EcoICRI GAGCTC 2 cut(s) 120, 151
EcoO109I RGGNCCY 2 cut(s) 1302, 1303
EcoRII CCWGG 6 cut(s) 212, 373, 417, 679, 967, 1044
EcoT14I CCWWGG 1 cut(s) 1165
EcoT22I ATGCAT 1 cut(s) 1539
EcoT38I GRGCYC 4 cut(s) 46, 122, 153, 1306
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 1165
FaeI CATG 2 cut(s) 207, 231
FalI AAGNNNNNCTT 1 cut(s) 1592
FatI CATG 2 cut(s) 203, 227
FauI CCCGC 1 cut(s) 1022
Fnu4HI GCNGC 2 cut(s) 104, 232
FokI GGATG 2 cut(s) 196, 868
FriOI GRGCYC 4 cut(s) 46, 122, 153, 1306
Fsp4HI GCNGC 2 cut(s) 104, 232
FspBI CTAG 3 cut(s) 1059, 1307, 1331
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 4 cut(s) 55, 102, 369, 1072
GluI GCNGC 2 cut(s) 104, 232
GsuI CTGGAG 3 cut(s) 167, 401, 1561
HaeII RGCGCY 2 cut(s) 57, 1074
HaeIII GGCC 7 cut(s) 187, 197, 243, 291, 399, 1304, 1486
HapII CCGG 9 cut(s) 154, 219, 267, 292, 304, 360, 749, 772, 893
HgaI GACGC 1 cut(s) 429
HhaI GCGC 4 cut(s) 56, 103, 370, 1073
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 207, 231
Hin6I GCGC 4 cut(s) 54, 101, 368, 1071
HinP1I GCGC 4 cut(s) 54, 101, 368, 1071
HindIII AAGCTT 1 cut(s) 629
HpaII CCGG 9 cut(s) 154, 219, 267, 292, 304, 360, 749, 772, 893
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 3 cut(s) 413, 1391, 1583
Hpy188I TCNGA 7 cut(s) 90, 124, 173, 724, 939, 945, 1153
Hpy188III TCNNGA 7 cut(s) 82, 569, 641, 878, 1004, 1435, 1493
Hpy8I GTNNAC 3 cut(s) 413, 1391, 1583
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 4 cut(s) 543, 615, 875, 1490
HpyCH4IV ACGT 2 cut(s) 1465, 1561
HpyCH4V TGCA 7 cut(s) 231, 413, 1249, 1446, 1475, 1511, 1537
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 240, 826, 1452
HpyF3I CTNAG 6 cut(s) 170, 431, 791, 830, 938, 1317
HpySE526I ACGT 2 cut(s) 1465, 1561
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 207, 231
HspAI GCGC 4 cut(s) 54, 101, 368, 1071
KasI GGCGCC 1 cut(s) 53
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Kzo9I GATC 4 cut(s) 571, 787, 1522, 1526
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 6 cut(s) 125, 130, 148, 156, 826, 1437
Lsp1109I GCAGC 2 cut(s) 115, 243
LweI GCATC 2 cut(s) 1524, 1546
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 3 cut(s) 1059, 1307, 1331
MaeII ACGT 2 cut(s) 1465, 1561
MaeIII GTNAC 6 cut(s) 16, 141, 223, 247, 761, 981
MalI GATC 4 cut(s) 573, 789, 1524, 1528
MboI GATC 4 cut(s) 571, 787, 1522, 1526
MboII GAAGA 5 cut(s) 150, 642, 956, 1193, 1283
MhlI GDGCHC 8 cut(s) 46, 122, 153, 415, 426, 964, 1306, 1434
MlsI TGGCCA 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 399
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 5 cut(s) 231, 255, 919, 949, 1386
MmeI TCCRAC 2 cut(s) 540, 923
Mox20I TGGCCA 1 cut(s) 399
Mph1103I ATGCAT 1 cut(s) 1539
MroNI GCCGGC 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 694
MscI TGGCCA 1 cut(s) 399
MseI TTAA 3 cut(s) 71, 1283, 1361
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 922
MspI CCGG 9 cut(s) 154, 219, 267, 292, 304, 360, 749, 772, 893
MspR9I CCNGG 9 cut(s) 214, 304, 361, 375, 419, 681, 772, 969, 1046
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 6 cut(s) 214, 375, 419, 681, 969, 1046
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 4 cut(s) 35, 240, 826, 1452
NaeI GCCGGC 1 cut(s) 293
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 3 cut(s) 304, 361, 772
NdeII GATC 4 cut(s) 571, 787, 1522, 1526
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 2 cut(s) 207, 231
NlaIV GGNNCC 8 cut(s) 55, 379, 598, 675, 691, 973, 1303, 1304
NmeAIII GCCGAG 2 cut(s) 269, 1374
NmuCI GTSAC 3 cut(s) 223, 247, 981
NsbI TGCGCA 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 1539
NspI RCATGY 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PctI GAATGC 1 cut(s) 589
PdiI GCCGGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 694
PfeI GAWTC 7 cut(s) 85, 317, 740, 745, 1340, 1552, 1572
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 2 cut(s) 105, 233
PleI GAGTC 5 cut(s) 230, 254, 918, 948, 1386
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 5 cut(s) 230, 254, 918, 948, 1386
Ppu21I YACGTR 1 cut(s) 1562
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 1612
Psp124BI GAGCTC 2 cut(s) 122, 153
Psp6I CCWGG 6 cut(s) 212, 373, 417, 679, 967, 1044
PspGI CCWGG 6 cut(s) 212, 373, 417, 679, 967, 1044
PspN4I GGNNCC 8 cut(s) 55, 379, 598, 675, 691, 973, 1303, 1304
PspOMI GGGCCC 1 cut(s) 1302
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 2 cut(s) 1230, 1416
RsaNI GTAC 2 cut(s) 1229, 1415
SacI GAGCTC 2 cut(s) 122, 153
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 3 cut(s) 71, 1283, 1361
SatI GCNGC 2 cut(s) 104, 232
Sau3AI GATC 4 cut(s) 571, 787, 1522, 1526
SchI GAGTC 5 cut(s) 231, 255, 919, 949, 1386
ScrFI CCNGG 9 cut(s) 214, 304, 361, 375, 419, 681, 772, 969, 1046
SduI GDGCHC 8 cut(s) 46, 122, 153, 415, 426, 964, 1306, 1434
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 2 cut(s) 1524, 1546
SfcI CTRYAG 1 cut(s) 1220
SfoI GGCGCC 1 cut(s) 55
SinI GGWCC 4 cut(s) 216, 378, 597, 884
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SnaBI TACGTA 1 cut(s) 1562
SsiI CCGC 1 cut(s) 1015
SspDI GGCGCC 1 cut(s) 53
SspMI CTAG 3 cut(s) 1059, 1307, 1331
SstI GAGCTC 2 cut(s) 122, 153
StyD4I CCNGG 9 cut(s) 212, 302, 359, 373, 417, 679, 770, 967, 1044
StyI CCWWGG 1 cut(s) 1165
TaiI ACGT 2 cut(s) 1468, 1564
TfiI GAWTC 7 cut(s) 85, 317, 740, 745, 1340, 1552, 1572
Tru1I TTAA 3 cut(s) 71, 1283, 1361
Tru9I TTAA 3 cut(s) 71, 1283, 1361
TscAI CASTG 3 cut(s) 256, 327, 1602
TseFI GTSAC 3 cut(s) 223, 247, 981
TseI GCWGC 2 cut(s) 103, 231
Tsp45I GTSAC 3 cut(s) 223, 247, 981
TspDTI ATGAA 5 cut(s) 17, 198, 1284, 1390, 1544
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 3 cut(s) 256, 327, 1602
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 4 cut(s) 216, 378, 597, 884
XapI RAATTY 5 cut(s) 564, 655, 950, 1130, 1279
XceI RCATGY 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 694
XspI CTAG 3 cut(s) 1059, 1307, 1331
Zsp2I ATGCAT 1 cut(s) 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.