pycom10g05940

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002531
Physical Location & Seq
Forward (+)
6666811 .. 6672000
5190 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g05940.1

Sequence Viewer

Length: 2712 bp
ATGATGTATCAGCGAAGCGTATTATCGTGTGCGTTGGTGTTGGTGGTGGTGGGGTTGCTGTTCGAGCCCACATTTGAGCAGCCCAGCCAGCCGCCATTGCCACTGGGTTTACCGGCTGAGAAGGCAGCGCTGCTGGACCTCAGGTCGTCGTTAGGCTTGAGGGGCAGGGACTGGCCGAGAAAGGCCGACCCTTGTTTCATTTGGAGAGGTGTGGAATGTCGGAATGGTAGAGTTGTTGGGATTAACATTTCGGGGTTTAGAAGGACTCGACTTGGCAGTCGAAACCCACAATTTTCTGTCAATGCTTTAGCCAATTTAACCCTTTTACACTCTTTCAATGCTTCCAATTTCCTGCTTCCGGGTTCAATTCCCGACTGGTTTGGACAACAACTCGTATCCCTACAAGTGCTTGATCTCACTGATTGTTCTGTGATTGGTTCTATTCCATTGAGTCTGGGAAATTCAACCAATCTGACTGCTCTGTATTTGTCACATAACAACCTTACCGGGACAATTCCTGCTACTTTGAGCCAGTTGCTAGGCCTTTCGGTTCTTGATCTTTCGCGAAATCACCTTACTGGATCCATTCCTACATCCTTTGGCAACCTTGGGAATCTGTCTGTGATTGACATTTCTGGGAATTACTTATCCGGGTCGATTCCTCCGGGCATTGGGACCCTGTTGAAGCTTCAGTATTTGAATCTTTCGAGCAACATGTTGTCTGCTTCGATACCTCCTCAACTTGGGAACCTTGGTAGCTTGGTTGACCTTGATCTAAGTGCCAATCTGTTGGCCAGGTCAGTGCCTGCAGATTTGAGGGGGTTAAGGAACTTGCAGAGAATGCTAGTTGCAGACAATTTGCTTAGTGGGACTTTGCCGGATAATCTGTTTCCAACACCGACGCAGTTGCAGGCCATAGTTCTGAGAAACAATGGTTTCACTGGTGGTCTTCCTAACGTGCTGTGGTCAATTCCCAAACTGAGCCTTCTTGATGTTTCTGGCAATAATTTCACTGGTATGCTGCCCAATTATAGCTCAAATGCTAATGGCACTGCTGCAGTATTCAATATTTCTCGGAATCTGTTCTATGGAGGTCTAACGCCTTTACTTGGAAGGTTTAGCGTCATTGATGTTTCGGGAAACTATTTTGAAGGTGGTGTACCAGGGTATGTGGGCACAAATGCATCTCTTAGCAGCAATTGCCTCCAAAATGTGAAGAATCAGAGGACATTGACGGAATGCTCTTCGTTCTATTCTGACAGGGGTTTGGCTTTTGATAATTTTGGACAGCCAAATTCTACACCTCCTGGGAACAGCAATAAAAAGGTGATCATCTTGGCAGCAGTTTTAGGGGGAGTTGGTTTGATTGTGCTTTTAGTGTTGTTTTGCGTGCTGCTATTCCTATGTTTACGCAAAAGGGGAACTACACCTCAAAGTGGAGTTGGCATGGGGCCAGTTACTGCAGGCAGCAGTCCACCACCTCCAGGAGCATCGATTAACTTTTCTAGTGTAGGAGATGCTTTCACATATCAGCAGCTGCTTCAAGCCGCTGGTGAATTCAACAATGCAAACTTGATAAAAAATGGCCACTCAGGGGATCTCTTCAGGGGGGTCCTAGAAAATGCGATCCCTGTGGTCATCAAACGGATTGATTTAAGCTCAACTAAAAAGGAAGCATACCTGCAGGAATTGGATTTCTTTAGTAAGGTGTCACATACAAGATTTGTTCCCCTTTTGGGACACTGTTTAGAGAACGAGAATGAGAAGTTCCTGGTCTACAAATATATGCCAAATGGGGACTTATCAAATTCATTGTTTAAGAAAACCAGCACAGAGGATGATAGTTTACAGTCGTTGGATTGGATAACGAGATTAAAGATTGCGTTAGGCGCAGCAGAGGGTCTCTCCTATCTGCATCATGAATGCAATCCCCCCCACGTGCACAGGGATGTTCAAGCAAGCAGTATACTTCTTGATGATAAATTTGAAGTGCGGCTAGGGAGCCTGAGTGATGTCTGTCCTCAGGAAGGGGACACCCATCAAAGCAGGATTACTAGGTTGCTACGCTTGCCACAGTCATCTGAACAAGGTGCTTCTGGTTCACCGGCAGCTTTGTGTGCCTATGATGTTTACTGTTTTGGGAAGGTGTTGCTTGAACTTGTAACAGGTAAGTTGGGCATAAGTGCTTCCAGTGATACGGAGGTGAAGGAATTGTTAGATCAGATACTACCATACATCAGTATATATGATAAAGAACTTGTGACAAAAATTGTCGATCCATCTCTGATTGTGGATGAGGACCTATTAGAGGAAGTCTGGGCCATGGCTGTCGTGGCCAGGTCCTGCCTAAATCCGAAGCCCTCAAGGCGTCCCCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTTTGAAGGTGGTGAGGGAAGATAGCTCCGGCTCTGCAAGGCTCAGAACAGCCTCTTCCAGAGGGTCTTGGAATGCTGCTGTGTTTGGTAGCTGGCGCAGCTCATCTGAGGTAGTGACTATCCCAGGAGCCTCAACTACGAAAGGGGAAGGAGCGAGTGGCTTAAAACATTCAGGAACCAGCGGTTCACAGGGAAGTGGTCCGAATGGTGGCGGGGAACATTCATCCTCACGCAGACGGCATTCTAGGGATATATTTCCGGAACCATCTGGTGTACATGATGTAGAAAAACCTGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

904

Amino Acids

97.08

Weight (kDa)

7.55

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 133 - 333 2.5e-13 Leucine-rich repeat region
LRR_8 PF13855 156 - 216 3.1e-10 Leucine rich repeat
Pkinase PF00069 524 - 797 3.4e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 798 6.4e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 1689
AccI GTMKAC 2 cut(s) 1776, 1966
AccII CGCG 1 cut(s) 565
AccIII TCCGGA 1 cut(s) 2665
AciI CCGC 5 cut(s) 92, 1548, 1993, 2589, 2619
AclWI GGATC 5 cut(s) 576, 589, 1605, 1621, 2270
AcoI YGGCCR 4 cut(s) 173, 792, 1585, 2334
AcsI RAATTY 5 cut(s) 460, 1294, 1556, 1807, 1982
AcuI CTGAAG 2 cut(s) 674, 1588
AcvI CACGTG 1 cut(s) 1939
AcyI GRCGYC 1 cut(s) 2368
AfaI GTAC 2 cut(s) 1161, 2682
AfeI AGCGCT 1 cut(s) 129
AflIII ACRYGT 1 cut(s) 714
AhdI GACNNNNNGTC 1 cut(s) 142
AjnI CCWGG 8 cut(s) 794, 1162, 1306, 1483, 1770, 2336, 2530, 2703
AluBI AGCT 9 cut(s) 688, 759, 1035, 1537, 1659, 2111, 2433, 2499, 2508
AluI AGCT 9 cut(s) 688, 759, 1035, 1537, 1659, 2111, 2433, 2499, 2508
Alw21I GWGCWC 1 cut(s) 1944
Alw26I GTCTC 1 cut(s) 1907
Alw44I GTGCAC 1 cut(s) 1940
AlwI GGATC 5 cut(s) 576, 589, 1605, 1621, 2270
AlwNI CAGNNNCTG 5 cut(s) 171, 806, 1460, 1537, 2343
Aor13HI TCCGGA 1 cut(s) 2665
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 9 cut(s) 173, 183, 541, 792, 912, 1451, 1585, 2319, 2334
ApaLI GTGCAC 1 cut(s) 1940
ApoI RAATTY 5 cut(s) 460, 1294, 1556, 1807, 1982
Asp700I GAANNNNTTC 1 cut(s) 1082
AspLEI GCGC 3 cut(s) 130, 1892, 2505
AspS9I GGNCC 8 cut(s) 136, 675, 1451, 1612, 2299, 2319, 2340, 2606
AsuC2I CCSGG 4 cut(s) 360, 508, 652, 666
AsuHPI GGTGA 6 cut(s) 563, 1339, 1565, 2094, 2215, 2431
AvaII GGWCC 6 cut(s) 136, 675, 1612, 2299, 2340, 2606
AxyI CCTNAGG 2 cut(s) 140, 2022
BaeGI GKGCMC 2 cut(s) 1178, 1944
BaeI ACNNNNGTAYC 2 cut(s) 2187, 2220
BalI TGGCCA 3 cut(s) 794, 1587, 2336
BamHI GGATCC 1 cut(s) 581
BanII GRGCYC 1 cut(s) 69
BarI GAAGNNNNNNTAC 2 cut(s) 1143, 1175
BbrPI CACGTG 1 cut(s) 1939
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 1944
BccI CCATC 3 cut(s) 2046, 2287, 2680
BceAI ACGGC 1 cut(s) 2660
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciT130I CCWGG 8 cut(s) 796, 1164, 1308, 1485, 1772, 2338, 2532, 2705
BciVI GTATCC 1 cut(s) 406
BclI TGATCA 1 cut(s) 1329
BcnI CCSGG 4 cut(s) 360, 508, 652, 666
BcoDI GTCTC 1 cut(s) 1907
BfaI CTAG 8 cut(s) 539, 845, 1506, 1616, 1997, 2055, 2652, 2710
BfmI CTRYAG 4 cut(s) 807, 1056, 1461, 1682
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 1689
BfuI GTATCC 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 2365
Bme18I GGWCC 6 cut(s) 136, 675, 1612, 2299, 2340, 2606
BmeRI GACNNNNNGTC 1 cut(s) 142
BmgT120I GGNCC 8 cut(s) 136, 675, 1451, 1612, 2299, 2319, 2340, 2606
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 4 cut(s) 1193, 1499, 1507, 1924
BmuI ACTGGG 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 941
BplI GAGNNNNNCTC 2 cut(s) 1889, 1921
BpmI CTGGAG 1 cut(s) 1467
BpuEI CTTGAG 3 cut(s) 178, 2347, 2375
BpuMI CCSGG 4 cut(s) 360, 508, 652, 666
Bsa29I ATCGAT 1 cut(s) 1493
BsaAI YACGTR 1 cut(s) 1939
BsaHI GRCGYC 1 cut(s) 2368
BsaI GGTCTC 1 cut(s) 1907
BsaJI CCNNGG 6 cut(s) 607, 751, 1163, 1307, 2322, 2530
BsaWI WCCGGW 1 cut(s) 2665
BsaXI ACNNNNNCTCC 2 cut(s) 1993, 2023
Bse118I RCCGGY 2 cut(s) 112, 2104
Bse1I ACTGG 9 cut(s) 108, 176, 380, 532, 583, 946, 1018, 1454, 2190
Bse21I CCTNAGG 2 cut(s) 140, 2022
Bse3DI GCAATG 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 2665
BseBI CCWGG 8 cut(s) 796, 1164, 1308, 1485, 1772, 2338, 2532, 2705
BseCI ATCGAT 1 cut(s) 1493
BseDI CCNNGG 6 cut(s) 607, 751, 1163, 1307, 2322, 2530
BseGI GGATG 5 cut(s) 593, 1843, 1954, 2299, 2630
BseMI GCAATG 1 cut(s) 95
BseMII CTCAG 9 cut(s) 108, 154, 914, 971, 1605, 1997, 2036, 2464, 2505
BseNI ACTGG 9 cut(s) 108, 176, 380, 532, 583, 946, 1018, 1454, 2190
BseRI GAGGAG 1 cut(s) 726
BseSI GKGCMC 2 cut(s) 1178, 1944
BseYI CCCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 565
BshFI GGCC 9 cut(s) 175, 185, 543, 794, 914, 1453, 1587, 2321, 2336
BshVI ATCGAT 1 cut(s) 1493
BsiHKAI GWGCWC 1 cut(s) 1944
BsiSI CCGG 9 cut(s) 113, 359, 507, 651, 665, 878, 2105, 2436, 2666
BslFI GGGAC 8 cut(s) 182, 523, 688, 883, 1752, 1811, 2045, 2355
BsmAI GTCTC 1 cut(s) 1907
BsmFI GGGAC 8 cut(s) 182, 523, 688, 883, 1752, 1811, 2045, 2355
BsmI GAATGC 5 cut(s) 846, 1244, 1928, 2485, 2647
BsnI GGCC 9 cut(s) 175, 185, 543, 794, 914, 1453, 1587, 2321, 2336
Bso31I GGTCTC 1 cut(s) 1907
Bsp1286I GDGCHC 3 cut(s) 69, 1178, 1944
Bsp13I TCCGGA 1 cut(s) 2665
Bsp1407I TGTACA 1 cut(s) 2680
Bsp143I GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
Bsp19I CCATGG 1 cut(s) 2322
Bsp68I TCGCGA 1 cut(s) 565
BspACI CCGC 5 cut(s) 92, 1548, 1993, 2589, 2619
BspANI GGCC 9 cut(s) 175, 185, 543, 794, 914, 1453, 1587, 2321, 2336
BspCNI CTCAG 9 cut(s) 109, 153, 915, 972, 1604, 1998, 2035, 2463, 2506
BspDI ATCGAT 1 cut(s) 1493
BspEI TCCGGA 1 cut(s) 2665
BspFNI CGCG 1 cut(s) 565
BspHI TCATGA 1 cut(s) 1918
BspMAI CTGCAG 4 cut(s) 811, 1060, 1465, 1686
BspMI ACCTGC 1 cut(s) 1689
BspPI GGATC 5 cut(s) 576, 589, 1605, 1621, 2270
BspQI GCTCTTC 1 cut(s) 1249
BspTNI GGTCTC 1 cut(s) 1907
BsrDI GCAATG 1 cut(s) 95
BsrFI RCCGGY 2 cut(s) 112, 2104
BsrGI TGTACA 1 cut(s) 2680
BsrI ACTGG 9 cut(s) 108, 176, 380, 532, 583, 946, 1018, 1454, 2190
BssAI RCCGGY 2 cut(s) 112, 2104
BssECI CCNNGG 6 cut(s) 607, 751, 1163, 1307, 2322, 2530
BssMI GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
BssNAI GTATAC 1 cut(s) 1967
BssNI GRCGYC 1 cut(s) 2368
BssT1I CCWWGG 3 cut(s) 607, 751, 2322
Bst1107I GTATAC 1 cut(s) 1967
Bst2UI CCWGG 8 cut(s) 796, 1164, 1308, 1485, 1772, 2338, 2532, 2705
Bst4CI ACNGT 4 cut(s) 1745, 1851, 2076, 2135
Bst6I CTCTTC 3 cut(s) 1249, 1607, 2467
BstACI GRCGYC 1 cut(s) 2368
BstAPI GCANNNNNTGC 2 cut(s) 841, 1200
BstAUI TGTACA 1 cut(s) 2680
BstBAI YACGTR 1 cut(s) 1939
BstC8I GCNNGC 8 cut(s) 89, 807, 912, 1391, 1465, 1960, 2069, 2501
BstDSI CCRYGG 1 cut(s) 2322
BstENI CCTNNNNNAGG 2 cut(s) 2025, 2306
BstF5I GGATG 5 cut(s) 593, 1843, 1954, 2299, 2630
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 3 cut(s) 130, 1892, 2505
BstKTI GATC 9 cut(s) 415, 559, 584, 775, 1332, 1600, 1629, 2221, 2278
BstMAI GTCTC 1 cut(s) 1907
BstMBI GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
BstNI CCWGG 8 cut(s) 796, 1164, 1308, 1485, 1772, 2338, 2532, 2705
BstNSI RCATGY 1 cut(s) 718
BstSFI CTRYAG 4 cut(s) 807, 1056, 1461, 1682
BstSLI GKGCMC 2 cut(s) 1178, 1944
BstUI CGCG 1 cut(s) 565
BstV2I GAAGAC 1 cut(s) 941
BstX2I RGATCY 2 cut(s) 581, 1597
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 2 cut(s) 581, 1597
BstZ17I GTATAC 1 cut(s) 1967
Bsu15I ATCGAT 1 cut(s) 1493
Bsu36I CCTNAGG 2 cut(s) 140, 2022
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 9 cut(s) 175, 185, 543, 794, 914, 1453, 1587, 2321, 2336
BsuTUI ATCGAT 1 cut(s) 1493
BtgI CCRYGG 1 cut(s) 2322
BtsCI GGATG 5 cut(s) 593, 1843, 1954, 2299, 2630
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 8 cut(s) 101, 417, 807, 939, 1011, 1050, 1741, 2197
BtuMI TCGCGA 1 cut(s) 565
BveI ACCTGC 1 cut(s) 1689
Cac8I GCNNGC 8 cut(s) 89, 807, 912, 1391, 1465, 1960, 2069, 2501
CaiI CAGNNNCTG 5 cut(s) 171, 806, 1460, 1537, 2343
CciI TCATGA 1 cut(s) 1918
CfoI GCGC 3 cut(s) 130, 1892, 2505
Cfr10I RCCGGY 2 cut(s) 112, 2104
Cfr13I GGNCC 8 cut(s) 136, 675, 1451, 1612, 2299, 2319, 2340, 2606
ClaI ATCGAT 1 cut(s) 1493
CseI GACGC 3 cut(s) 910, 1111, 2357
Csp6I GTAC 2 cut(s) 1160, 2681
CviAII CATG 5 cut(s) 715, 1447, 1919, 2323, 2684
CviQI GTAC 2 cut(s) 1160, 2681
DpnI GATC 9 cut(s) 414, 558, 583, 774, 1331, 1599, 1628, 2220, 2277
DpnII GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
DrdI GACNNNNNNGTC 1 cut(s) 276
DriI GACNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 276
EaeI YGGCCR 4 cut(s) 173, 792, 1585, 2334
Eam1104I CTCTTC 3 cut(s) 1249, 1607, 2467
Eam1105I GACNNNNNGTC 1 cut(s) 142
EarI CTCTTC 3 cut(s) 1249, 1607, 2467
Eco130I CCWWGG 3 cut(s) 607, 751, 2322
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 1907
Eco47I GGWCC 6 cut(s) 136, 675, 1612, 2299, 2340, 2606
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 674, 1588
Eco72I CACGTG 1 cut(s) 1939
Eco81I CCTNAGG 2 cut(s) 140, 2022
EcoNI CCTNNNNNAGG 2 cut(s) 2025, 2306
EcoO109I RGGNCCY 4 cut(s) 675, 1612, 2299, 2340
EcoRI GAATTC 1 cut(s) 1556
EcoRII CCWGG 8 cut(s) 794, 1162, 1306, 1483, 1770, 2336, 2530, 2703
EcoT14I CCWWGG 3 cut(s) 607, 751, 2322
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 3 cut(s) 607, 751, 2322
FaeI CATG 5 cut(s) 718, 1450, 1922, 2326, 2687
FaqI GGGAC 8 cut(s) 182, 523, 688, 883, 1752, 1811, 2045, 2355
FatI CATG 5 cut(s) 714, 1446, 1918, 2322, 2683
FauI CCCGC 1 cut(s) 2612
FbaI TGATCA 1 cut(s) 1329
FblI GTMKAC 2 cut(s) 1776, 1966
FokI GGATG 5 cut(s) 580, 1850, 1961, 2306, 2617
FriOI GRGCYC 1 cut(s) 69
FspBI CTAG 8 cut(s) 539, 845, 1506, 1616, 1997, 2055, 2652, 2710
GlaI GCGC 3 cut(s) 129, 1891, 2504
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 1467
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 9 cut(s) 175, 185, 543, 794, 914, 1453, 1587, 2321, 2336
HapII CCGG 9 cut(s) 113, 359, 507, 651, 665, 878, 2105, 2436, 2666
HgaI GACGC 3 cut(s) 910, 1111, 2357
HhaI GCGC 3 cut(s) 130, 1892, 2505
Hin1I GRCGYC 1 cut(s) 2368
Hin1II CATG 5 cut(s) 718, 1450, 1922, 2326, 2687
Hin6I GCGC 3 cut(s) 128, 1890, 2503
HinP1I GCGC 3 cut(s) 128, 1890, 2503
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 7 cut(s) 265, 451, 613, 658, 700, 1078, 1219
HpaII CCGG 9 cut(s) 113, 359, 507, 651, 665, 878, 2105, 2436, 2666
HphI GGTGA 6 cut(s) 563, 1339, 1565, 2094, 2215, 2431
Hpy99I CGWCG 2 cut(s) 151, 904
HpyCH4III ACNGT 4 cut(s) 1745, 1851, 2076, 2135
HpyCH4IV ACGT 2 cut(s) 957, 1938
HpySE526I ACGT 2 cut(s) 957, 1938
Hsp92I GRCGYC 1 cut(s) 2368
Hsp92II CATG 5 cut(s) 718, 1450, 1922, 2326, 2687
HspAI GCGC 3 cut(s) 128, 1890, 2503
KflI GGGWCCC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 2665
Ksp22I TGATCA 1 cut(s) 1329
Kzo9I GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
LguI GCTCTTC 1 cut(s) 1249
LmnI GCTCC 5 cut(s) 1487, 2001, 2438, 2534, 2558
LweI GCATC 4 cut(s) 1193, 1499, 1507, 1924
MaeI CTAG 8 cut(s) 539, 845, 1506, 1616, 1997, 2055, 2652, 2710
MaeII ACGT 2 cut(s) 957, 1938
MaeIII GTNAC 6 cut(s) 489, 1456, 1710, 2161, 2260, 2521
MalI GATC 9 cut(s) 414, 558, 583, 774, 1331, 1599, 1628, 2220, 2277
MboI GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
MboII GAAGA 6 cut(s) 941, 1228, 1236, 1594, 2438, 2454
MfeI CAATTG 1 cut(s) 1198
MflI RGATCY 2 cut(s) 581, 1597
MhlI GDGCHC 3 cut(s) 69, 1178, 1944
MlsI TGGCCA 3 cut(s) 794, 1587, 2336
MluNI TGGCCA 3 cut(s) 794, 1587, 2336
MlyI GAGTC 2 cut(s) 259, 460
MmeI TCCRAC 3 cut(s) 200, 917, 1836
Mox20I TGGCCA 3 cut(s) 794, 1587, 2336
Mph1103I ATGCAT 1 cut(s) 1186
MroI TCCGGA 1 cut(s) 2665
MroXI GAANNNNTTC 1 cut(s) 1082
MscI TGGCCA 3 cut(s) 794, 1587, 2336
MseI TTAA 8 cut(s) 243, 317, 824, 1497, 1655, 1818, 1874, 2570
MslI CAYNNNNRTG 2 cut(s) 1016, 1947
Msp20I TGGCCA 3 cut(s) 794, 1587, 2336
MspA1I CMGCKG 3 cut(s) 1537, 1550, 2589
MspI CCGG 9 cut(s) 113, 359, 507, 651, 665, 878, 2105, 2436, 2666
MunI CAATTG 1 cut(s) 1198
Mva1269I GAATGC 5 cut(s) 846, 1244, 1928, 2485, 2647
MvaI CCWGG 8 cut(s) 796, 1164, 1308, 1485, 1772, 2338, 2532, 2705
MvnI CGCG 1 cut(s) 565
NciI CCSGG 4 cut(s) 360, 508, 652, 666
NcoI CCATGG 1 cut(s) 2322
NdeII GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
NlaIII CATG 5 cut(s) 718, 1450, 1922, 2326, 2687
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 4 cut(s) 489, 1710, 2260, 2521
NruI TCGCGA 1 cut(s) 565
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 1 cut(s) 718
PagI TCATGA 1 cut(s) 1918
PceI AGGCCT 1 cut(s) 543
PciI ACATGT 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 1249
PctI GAATGC 5 cut(s) 846, 1244, 1928, 2485, 2647
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 5 cut(s) 613, 658, 700, 1078, 1219
PfoI TCCNGGA 1 cut(s) 1483
PleI GAGTC 2 cut(s) 259, 459
PmaCI CACGTG 1 cut(s) 1939
PmlI CACGTG 1 cut(s) 1939
PpsI GAGTC 2 cut(s) 259, 459
Ppu21I YACGTR 1 cut(s) 1939
PpuMI RGGWCCY 4 cut(s) 675, 1612, 2299, 2340
PscI ACATGT 1 cut(s) 714
Psp5II RGGWCCY 4 cut(s) 675, 1612, 2299, 2340
Psp6I CCWGG 8 cut(s) 794, 1162, 1306, 1483, 1770, 2336, 2530, 2703
PspCI CACGTG 1 cut(s) 1939
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 8 cut(s) 794, 1162, 1306, 1483, 1770, 2336, 2530, 2703
PspPI GGNCC 8 cut(s) 136, 675, 1451, 1612, 2299, 2319, 2340, 2606
PspPPI RGGWCCY 4 cut(s) 675, 1612, 2299, 2340
PstI CTGCAG 4 cut(s) 811, 1060, 1465, 1686
PstNI CAGNNNCTG 5 cut(s) 171, 806, 1460, 1537, 2343
PsuI RGATCY 2 cut(s) 581, 1597
PvuII CAGCTG 1 cut(s) 1537
RruI TCGCGA 1 cut(s) 565
RsaI GTAC 2 cut(s) 1161, 2682
RsaNI GTAC 2 cut(s) 1160, 2681
RseI CAYNNNNRTG 2 cut(s) 1016, 1947
SapI GCTCTTC 1 cut(s) 1249
SaqAI TTAA 8 cut(s) 243, 317, 824, 1497, 1655, 1818, 1874, 2570
Sau3AI GATC 9 cut(s) 412, 556, 581, 772, 1329, 1597, 1626, 2218, 2275
Sau96I GGNCC 8 cut(s) 136, 675, 1451, 1612, 2299, 2319, 2340, 2606
SbfI CCTGCAGG 1 cut(s) 1686
SchI GAGTC 2 cut(s) 259, 460
SdaI CCTGCAGG 1 cut(s) 1686
SduI GDGCHC 3 cut(s) 69, 1178, 1944
SfaNI GCATC 4 cut(s) 1193, 1499, 1507, 1924
SfcI CTRYAG 4 cut(s) 807, 1056, 1461, 1682
SinI GGWCC 6 cut(s) 136, 675, 1612, 2299, 2340, 2606
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1947
SmlI CTYRAG 3 cut(s) 157, 2362, 2390
SmoI CTYRAG 3 cut(s) 157, 2362, 2390
Sse8387I CCTGCAGG 1 cut(s) 1686
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 5 cut(s) 92, 1548, 1993, 2589, 2619
SspI AATATT 1 cut(s) 1069
SspMI CTAG 8 cut(s) 539, 845, 1506, 1616, 1997, 2055, 2652, 2710
StuI AGGCCT 1 cut(s) 543
StyI CCWWGG 3 cut(s) 607, 751, 2322
TaaI ACNGT 4 cut(s) 1745, 1851, 2076, 2135
TaiI ACGT 2 cut(s) 960, 1941
TaqI TCGA 8 cut(s) 63, 268, 280, 656, 707, 728, 1493, 2274
TatI WGTACW 1 cut(s) 2680
TauI GCSGC 3 cut(s) 94, 1550, 1996
TfiI GAWTC 5 cut(s) 613, 658, 700, 1078, 1219
Tru1I TTAA 8 cut(s) 243, 317, 824, 1497, 1655, 1818, 1874, 2570
Tru9I TTAA 8 cut(s) 243, 317, 824, 1497, 1655, 1818, 1874, 2570
TscAI CASTG 8 cut(s) 108, 424, 807, 946, 1018, 1057, 1748, 2197
TseFI GTSAC 4 cut(s) 489, 1710, 2260, 2521
Tsp45I GTSAC 4 cut(s) 489, 1710, 2260, 2521
TspDTI ATGAA 4 cut(s) 187, 1800, 1935, 2619
TspGWI ACGGA 3 cut(s) 1250, 1660, 2213
TspRI CASTG 8 cut(s) 108, 424, 807, 946, 1018, 1057, 1748, 2197
VneI GTGCAC 1 cut(s) 1940
VpaK11BI GGWCC 6 cut(s) 136, 675, 1612, 2299, 2340, 2606
XagI CCTNNNNNAGG 2 cut(s) 2025, 2306
XapI RAATTY 5 cut(s) 460, 1294, 1556, 1807, 1982
XceI RCATGY 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 2329
XmiI GTMKAC 2 cut(s) 1776, 1966
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 8 cut(s) 539, 845, 1506, 1616, 1997, 2055, 2652, 2710
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.