MD05G1065800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002531
Physical Location & Seq
Forward (+)
12309369 .. 12316041
6673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1065800.v1.1.491

Sequence Viewer

Length: 2733 bp
ATGGTGGATCAGCGAAGCGTTGTATCGTGTGCCTTCTTGTTGGTGGTGGTGGGGTTGCTGTTTGAGCCCACATTTGAGCAGCCGAGCCAGCCGCCAATGCCACTGGTTTCACCGGCTGAGCGGGCGGCGCTGCTGGAACTCAGATCGTCTTTAGGGTTGAGAGGGAAAGACTGGCCGATAAAGGCAGACCCTTGCTTCATCTGGAAAGGTGTGGAATGCCGGAATGGTAGAGTTGTTGGGATTAACATTTCTGGGTTTAGAAGGACTCGACTTGGCAGTCGAAACCCACAATTTTCTGTCAATGCTTTGGCCAATATAACCCTTTTGCAGTCTTTCAATGCTTCCAGTTTCCTGCTTCCGGGTTCGATTCCCGACTGGTTTGGACAACAACTCATGTCCCTACAAGTGCTTGATCTCACTGCTTGTTCTGTGATTGGTGCTATTCCATTGAGTCTGGGAAATTCAACCAATCTGACTGCTCTGTATTTGTCTTATAACAACCTTACTGGGACAATTCCTGCTAGTTTGAGCCAGCTGCTAGGCCTTTCAGTTGTTGATCTTTCGCAAAATCGCCTTACTGGATCCATTCCTACATCCTTTGGCAACCTTGGGAATCTGTCTGTGCTTGACATTTCTGGGAATTACTTATCGGGGTCGATTTCTCCCGGCATTGGGACCCTGTTGAAGCTTCAGTATTTGAATCTTTCAAACAACATGTTGTCTGCTTCAATACCTCCGCAACTTGGGGACCTTGGTAGCTTGGTTGACCTTGATCTAAGTGCCAATCTATTGGCCGGTTCAGTGCCTTCTGATTTGAGGGGGTTAAGGAACTTGCAGAAAATGATAGTTGCAGACAATTTGCTGAGTGGGATTTTGCCGGATAATCTGTTTCCAACACTGACGCAGTTGCAGGTCATAGTTCTGAGAAACAATGGTTTCACGGGTGGTCTTCCTAATGTCCTGTGGTCAATTCCCAAATTGAGCCTTCTTGATGTTTCTGGCAATAATTTCACTGGTATGCTGCCCAATTCTAGCTCGAATGCTAATGCCACTGCTGCAGTATTCAATATTTCTCGGAATCTGTTCTATGGAGGTCTAACGCCTTTACTTGGAAGGTTCAGCGATATTGATATTTCGGGAAACTATTTTGAAGGTGGTGTGCCAGGGTATGTGGGCACAAATGCATCTCTTGGCAGCAATTGCCTCCAAAATGTGAAGAATCAGAGGACATTGAAAGAATGCTCTTCGTTCTATGCTGATAGGGGTTTGGCTTTTGATAATTTTGGACAGCCAGAGTCTACACAACCACCTCTAGTAAAACCTCCTGGGAAGAGCAATAAAAAGGTGATCATCTTGGCAGCAGTTTTAGGGGGAGTTGGTTTGATTGTTCTTTTAGTGTTGTTTTGCGTGGTGCTACTCCTATGTTTACGCAAGAGGGGAACAACAACTCAAAGAGGAGTTGACGTGGGGCTAGCTACTGCAGGCGGCAGTCCACCACCTCCAGGAGCACCGATTAACTTTTCTAGTGTAGGAGATGCTTTCACATATCAGCAGCTGCTTCAAGCCACGGGTGAATTCAACAATGCAAACTTGCTAAAAAATGGCCACTCAGGGGATCTCTTCAGGGGGGTCTTGGAAAATGGGATCCCTGTGGTCATTAAACGGATTGATTTATGCTCGATTAAAAAGGAAGCATACCTACAGGAATTGGATTTCTTTAGTAAGGTGTCACATGCAAGATTTGTTCCCCTTCTGGGACACTGTTTAGAGAATGAGAATGAGAAGTTCCTGGTCTACAAATCTATGCCAAATGGAGACTTATCGAATTCCTTGTTCAAGAAAACCAGCACAGAGGATGATAGTTTACAGTCGTTGGATTGGATAACGAGATTAAAGATTGCATTAGGCGGAGCAGAGGGTCTCTCCTATCTGCATCATGAATGCAATCCCCCCTACGTGCACAGGGATGTTCAAGCAAGTAGTATACTTCTTGATGATAAATTTGAAGTGCGGCTAGGGAGCCTGAGTGATGTCTGTTCTCAAGAAGGGGACACTCATCAAAGCAGGATTACCAGGTTGCTGCGGTTGCCACAGTCATCTGAACATGGTGCTTCTGGTTCCCCAACAGCAGTGTGTGCCTATGATGTTTACTGTTTTGGGAAGGTGTTACTTGAACTCGTAACAGGTAAGTTGGGCATAAGTGCTTCTAGTGACACCAACGTGAAGGAATTGTTAGATCAGATACTACCATACATCAGTATATATGATAAAGAACTTGTGACAAAAATTGTGGATCCATCACTGATTGTGGATGAGGACCTATTAGAGGAAGTGTGGGCCATGGCTGTTGTGGCCAGGTCCTGCCTAAATCCGAAGCCCTCAAGGCGTCCCCTAATGAGATATATTCTCAAGGCTTTGGAAAACCCTTTGAAGGTGGTGAGGGAAGATAGCTCTGGTTCTGCTAGGCTCAGAACAGCTTCTTCCAGAGGGTCTTGGAATGCTGCTGTGTTTGGTAGCTGGCGCAGCTCATCCGAGGTAGTGGTCATCCCAGGAGCCTCAACTATGAAAGGGGAAGGAGCAAGTGGCTTAAAACATTCAGGAACCAGCGGTTCGCAGGGAAGTGGTCCGACAAATGGTGGCGGGGAACATTCATCCTCACGCAGACGGCATTCTAGGGATATATGTCCGGAACCATCTGGTGTACATGATGTAGAAAAACCTGACCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

911

Amino Acids

97.59

Weight (kDa)

7.14

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 156 - 216 1.7e-09 Leucine rich repeat
LRR_14 PF23598 206 - 309 5.1e-07 Leucine-rich repeat region
LRR_8 PF13855 276 - 337 2.6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 530 - 804 4.2e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 532 - 803 8.3e-14 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AasI GACNNNNNNGTC 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 901
AccBSI CCGCTC 1 cut(s) 121
AccI GTMKAC 3 cut(s) 1298, 1794, 1984
AccIII TCCGGA 1 cut(s) 2686
AclWI GGATC 8 cut(s) 15, 576, 589, 1623, 1639, 1652, 2288, 2301
AcoI YGGCCR 5 cut(s) 173, 309, 792, 1603, 2352
AcsI RAATTY 4 cut(s) 460, 1574, 1825, 2000
AcuI CTGAAG 2 cut(s) 674, 1606
AcyI GRCGYC 1 cut(s) 2386
AfaI GTAC 1 cut(s) 2703
AflIII ACRYGT 1 cut(s) 714
AjiI CACGTC 1 cut(s) 1465
AjnI CCWGG 8 cut(s) 1162, 1324, 1501, 1788, 2072, 2354, 2548, 2724
AleI CACNNNNGTG 1 cut(s) 2219
Alw21I GWGCWC 2 cut(s) 1510, 1962
Alw26I GTCTC 2 cut(s) 1809, 1925
Alw44I GTGCAC 1 cut(s) 1958
AlwI GGATC 8 cut(s) 15, 576, 589, 1623, 1639, 1652, 2288, 2301
AlwNI CAGNNNCTG 2 cut(s) 1555, 2361
Aor13HI TCCGGA 1 cut(s) 2686
AoxI GGCC 7 cut(s) 173, 309, 541, 792, 1603, 2337, 2352
ApaLI GTGCAC 1 cut(s) 1958
ApoI RAATTY 4 cut(s) 460, 1574, 1825, 2000
Asp700I GAANNNNTTC 2 cut(s) 1082, 2476
AspLEI GCGC 2 cut(s) 130, 2523
AspS9I GGNCC 6 cut(s) 675, 748, 2317, 2337, 2358, 2624
AsuC2I CCSGG 2 cut(s) 360, 666
AsuHPI GGTGA 4 cut(s) 102, 1357, 1583, 2449
AsuNHI GCTAGC 1 cut(s) 1471
AvaII GGWCC 5 cut(s) 675, 748, 2317, 2358, 2624
BaeGI GKGCMC 2 cut(s) 1178, 1962
BalI TGGCCA 3 cut(s) 311, 1605, 2354
BamHI GGATCC 3 cut(s) 581, 1644, 2293
BanII GRGCYC 1 cut(s) 69
BarI GAAGNNNNNNTAC 2 cut(s) 1683, 1715
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 2 cut(s) 1510, 1962
BccI CCATC 2 cut(s) 2305, 2701
BceAI ACGGC 1 cut(s) 2681
BciT130I CCWGG 8 cut(s) 1164, 1326, 1503, 1790, 2074, 2356, 2550, 2726
BclI TGATCA 1 cut(s) 1347
BcnI CCSGG 2 cut(s) 360, 666
BcoDI GTCTC 2 cut(s) 1809, 1925
BfmI CTRYAG 3 cut(s) 1056, 1479, 1700
BfoI RGCGCY 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 2383
BlpI GCTNAGC 1 cut(s) 117
Bme18I GGWCC 5 cut(s) 675, 748, 2317, 2358, 2624
BmgBI CACGTC 1 cut(s) 1465
BmgT120I GGNCC 6 cut(s) 675, 748, 2317, 2337, 2358, 2624
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 3 cut(s) 1193, 1525, 1942
BmtI GCTAGC 1 cut(s) 1475
BmuI ACTGGG 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 941
BplI GAGNNNNNCTC 2 cut(s) 1907, 1939
BpmI CTGGAG 1 cut(s) 1485
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 3 cut(s) 2025, 2365, 2393
BpuMI CCSGG 2 cut(s) 360, 666
BsaAI YACGTR 1 cut(s) 1957
BsaHI GRCGYC 1 cut(s) 2386
BsaI GGTCTC 1 cut(s) 1925
BsaJI CCNNGG 8 cut(s) 607, 751, 1163, 1325, 1566, 2340, 2532, 2548
BsaWI WCCGGW 1 cut(s) 2686
BsaXI ACNNNNNCTCC 4 cut(s) 1449, 1479, 2011, 2041
Bse118I RCCGGY 2 cut(s) 112, 794
Bse1I ACTGG 7 cut(s) 108, 176, 345, 380, 511, 583, 1018
BseAI TCCGGA 1 cut(s) 2686
BseBI CCWGG 8 cut(s) 1164, 1326, 1503, 1790, 2074, 2356, 2550, 2726
BseDI CCNNGG 8 cut(s) 607, 751, 1163, 1325, 1566, 2340, 2532, 2548
BseGI GGATG 7 cut(s) 593, 1861, 1972, 2317, 2528, 2544, 2651
BseMII CTCAG 7 cut(s) 108, 154, 854, 914, 1623, 2015, 2482
BseNI ACTGG 7 cut(s) 108, 176, 345, 380, 511, 583, 1018
BseRI GAGGAG 1 cut(s) 1470
BseSI GKGCMC 2 cut(s) 1178, 1962
BshFI GGCC 7 cut(s) 175, 311, 543, 794, 1605, 2339, 2354
BsiHKAI GWGCWC 2 cut(s) 1510, 1962
BsiSI CCGG 7 cut(s) 113, 220, 359, 666, 795, 878, 2687
BslFI GGGAC 7 cut(s) 382, 523, 688, 761, 1770, 2063, 2373
BsmAI GTCTC 2 cut(s) 1809, 1925
BsmFI GGGAC 7 cut(s) 382, 523, 688, 761, 1770, 2063, 2373
BsmI GAATGC 6 cut(s) 221, 1045, 1244, 1946, 2503, 2668
BsnI GGCC 7 cut(s) 175, 311, 543, 794, 1605, 2339, 2354
Bso31I GGTCTC 1 cut(s) 1925
Bsp1286I GDGCHC 4 cut(s) 69, 1178, 1510, 1962
Bsp13I TCCGGA 1 cut(s) 2686
Bsp1407I TGTACA 1 cut(s) 2701
Bsp1720I GCTNAGC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 2340
BspANI GGCC 7 cut(s) 175, 311, 543, 794, 1605, 2339, 2354
BspCNI CTCAG 7 cut(s) 109, 153, 855, 915, 1622, 2016, 2481
BspEI TCCGGA 1 cut(s) 2686
BspHI TCATGA 1 cut(s) 1936
BspMAI CTGCAG 2 cut(s) 1060, 1483
BspMI ACCTGC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 1475
BspPI GGATC 8 cut(s) 15, 576, 589, 1623, 1639, 1652, 2288, 2301
BspQI GCTCTTC 2 cut(s) 1249, 1325
BspTNI GGTCTC 1 cut(s) 1925
BsrBI CCGCTC 1 cut(s) 121
BsrFI RCCGGY 2 cut(s) 112, 794
BsrGI TGTACA 1 cut(s) 2701
BsrI ACTGG 7 cut(s) 108, 176, 345, 380, 511, 583, 1018
BssAI RCCGGY 2 cut(s) 112, 794
BssECI CCNNGG 8 cut(s) 607, 751, 1163, 1325, 1566, 2340, 2532, 2548
BssNAI GTATAC 1 cut(s) 1985
BssNI GRCGYC 1 cut(s) 2386
BssT1I CCWWGG 3 cut(s) 607, 751, 2340
Bst1107I GTATAC 1 cut(s) 1985
Bst2UI CCWGG 8 cut(s) 1164, 1326, 1503, 1790, 2074, 2356, 2550, 2726
Bst4CI ACNGT 4 cut(s) 1763, 1869, 2094, 2153
Bst6I CTCTTC 3 cut(s) 1249, 1325, 1625
BstACI GRCGYC 1 cut(s) 2386
BstAPI GCANNNNNTGC 2 cut(s) 1200, 2135
BstAUI TGTACA 1 cut(s) 2701
BstBAI YACGTR 1 cut(s) 1957
BstC8I GCNNGC 6 cut(s) 89, 123, 533, 1473, 1483, 2519
BstDEI CTNAG 8 cut(s) 117, 140, 776, 863, 923, 1609, 2024, 2468
BstDSI CCRYGG 2 cut(s) 1566, 2340
BstENI CCTNNNNNAGG 1 cut(s) 2324
BstF5I GGATG 7 cut(s) 593, 1861, 1972, 2317, 2528, 2544, 2651
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 130, 2523
BstMAI GTCTC 2 cut(s) 1809, 1925
BstNI CCWGG 8 cut(s) 1164, 1326, 1503, 1790, 2074, 2356, 2550, 2726
BstNSI RCATGY 2 cut(s) 718, 1736
BstSFI CTRYAG 3 cut(s) 1056, 1479, 1700
BstSLI GKGCMC 2 cut(s) 1178, 1962
BstV2I GAAGAC 1 cut(s) 941
BstX2I RGATCY 4 cut(s) 581, 1615, 1644, 2293
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 4 cut(s) 581, 1615, 1644, 2293
BstZ17I GTATAC 1 cut(s) 1985
BsuRI GGCC 7 cut(s) 175, 311, 543, 794, 1605, 2339, 2354
BtgI CCRYGG 2 cut(s) 1566, 2340
BtrI CACGTC 1 cut(s) 1465
BtsCI GGATG 7 cut(s) 593, 1861, 1972, 2317, 2528, 2544, 2651
BtsI GCAGTG 3 cut(s) 417, 1050, 2136
BtsIMutI CAGTG 9 cut(s) 101, 417, 807, 896, 1011, 1050, 1759, 2136, 2300
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 6 cut(s) 89, 123, 533, 1473, 1483, 2519
CaiI CAGNNNCTG 2 cut(s) 1555, 2361
CciI TCATGA 1 cut(s) 1936
CfoI GCGC 2 cut(s) 130, 2523
Cfr10I RCCGGY 2 cut(s) 112, 794
Cfr13I GGNCC 6 cut(s) 675, 748, 2317, 2337, 2358, 2624
CseI GACGC 2 cut(s) 910, 2375
CsiI ACCWGGT 1 cut(s) 2072
Csp6I GTAC 1 cut(s) 2702
CspCI CAANNNNNGTGG 2 cut(s) 2271, 2306
CviAII CATG 7 cut(s) 394, 715, 1733, 1937, 2105, 2341, 2705
CviQI GTAC 1 cut(s) 2702
DdeI CTNAG 8 cut(s) 117, 140, 776, 863, 923, 1609, 2024, 2468
DrdI GACNNNNNNGTC 1 cut(s) 276
DseDI GACNNNNNNGTC 1 cut(s) 276
EaeI YGGCCR 5 cut(s) 173, 309, 792, 1603, 2352
Eam1104I CTCTTC 3 cut(s) 1249, 1325, 1625
EarI CTCTTC 3 cut(s) 1249, 1325, 1625
EciI GGCGGA 1 cut(s) 1923
Eco130I CCWWGG 3 cut(s) 607, 751, 2340
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 1925
Eco47I GGWCC 5 cut(s) 675, 748, 2317, 2358, 2624
Eco57I CTGAAG 2 cut(s) 674, 1606
EcoNI CCTNNNNNAGG 1 cut(s) 2324
EcoO109I RGGNCCY 4 cut(s) 675, 748, 2317, 2358
EcoRI GAATTC 2 cut(s) 1574, 1825
EcoRII CCWGG 8 cut(s) 1162, 1324, 1501, 1788, 2072, 2354, 2548, 2724
EcoT14I CCWWGG 3 cut(s) 607, 751, 2340
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 3 cut(s) 607, 751, 2340
FaeI CATG 7 cut(s) 397, 718, 1736, 1940, 2108, 2344, 2708
FaqI GGGAC 7 cut(s) 382, 523, 688, 761, 1770, 2063, 2373
FatI CATG 7 cut(s) 393, 714, 1732, 1936, 2104, 2340, 2704
FauI CCCGC 2 cut(s) 114, 2633
FbaI TGATCA 1 cut(s) 1347
FblI GTMKAC 3 cut(s) 1298, 1794, 1984
FokI GGATG 7 cut(s) 580, 1868, 1979, 2324, 2515, 2531, 2638
FriOI GRGCYC 1 cut(s) 69
GlaI GCGC 2 cut(s) 129, 2522
GsuI CTGGAG 1 cut(s) 1485
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 7 cut(s) 175, 311, 543, 794, 1605, 2339, 2354
HapII CCGG 7 cut(s) 113, 220, 359, 666, 795, 878, 2687
HgaI GACGC 2 cut(s) 910, 2375
HhaI GCGC 2 cut(s) 130, 2523
Hin1I GRCGYC 1 cut(s) 2386
Hin1II CATG 7 cut(s) 397, 718, 1736, 1940, 2108, 2344, 2708
Hin6I GCGC 2 cut(s) 128, 2521
HinP1I GCGC 2 cut(s) 128, 2521
HincII GTYRAC 2 cut(s) 766, 1462
HindII GTYRAC 2 cut(s) 766, 1462
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 8 cut(s) 265, 367, 451, 613, 700, 1078, 1219, 1295
HpaII CCGG 7 cut(s) 113, 220, 359, 666, 795, 878, 2687
HphI GGTGA 4 cut(s) 102, 1357, 1583, 2449
HpyCH4III ACNGT 4 cut(s) 1763, 1869, 2094, 2153
HpyCH4IV ACGT 3 cut(s) 1464, 1956, 2220
HpyF3I CTNAG 8 cut(s) 117, 140, 776, 863, 923, 1609, 2024, 2468
HpySE526I ACGT 3 cut(s) 1464, 1956, 2220
Hsp92I GRCGYC 1 cut(s) 2386
Hsp92II CATG 7 cut(s) 397, 718, 1736, 1940, 2108, 2344, 2708
HspAI GCGC 2 cut(s) 128, 2521
KflI GGGWCCC 1 cut(s) 675
Kpn2I TCCGGA 1 cut(s) 2686
Ksp22I TGATCA 1 cut(s) 1347
LguI GCTCTTC 2 cut(s) 1249, 1325
LmnI GCTCC 5 cut(s) 1505, 1910, 2019, 2552, 2576
LweI GCATC 3 cut(s) 1193, 1525, 1942
MabI ACCWGGT 1 cut(s) 2072
MaeII ACGT 3 cut(s) 1464, 1956, 2220
MaeIII GTNAC 5 cut(s) 1728, 2166, 2179, 2210, 2278
MbiI CCGCTC 1 cut(s) 121
MboII GAAGA 7 cut(s) 941, 1228, 1236, 1342, 1612, 2456, 2472
MfeI CAATTG 1 cut(s) 1198
MflI RGATCY 4 cut(s) 581, 1615, 1644, 2293
MhlI GDGCHC 4 cut(s) 69, 1178, 1510, 1962
MlsI TGGCCA 3 cut(s) 311, 1605, 2354
MluNI TGGCCA 3 cut(s) 311, 1605, 2354
MlyI GAGTC 3 cut(s) 259, 460, 1304
MmeI TCCRAC 3 cut(s) 917, 1854, 2651
Mox20I TGGCCA 3 cut(s) 311, 1605, 2354
Mph1103I ATGCAT 1 cut(s) 1186
MroI TCCGGA 1 cut(s) 2686
MroXI GAANNNNTTC 2 cut(s) 1082, 2476
MscI TGGCCA 3 cut(s) 311, 1605, 2354
MseI TTAA 7 cut(s) 243, 824, 1515, 1659, 1683, 1892, 2588
MslI CAYNNNNRTG 3 cut(s) 1016, 1965, 2219
Msp20I TGGCCA 3 cut(s) 311, 1605, 2354
MspA1I CMGCKG 3 cut(s) 535, 1555, 2607
MspI CCGG 7 cut(s) 113, 220, 359, 666, 795, 878, 2687
MunI CAATTG 1 cut(s) 1198
Mva1269I GAATGC 6 cut(s) 221, 1045, 1244, 1946, 2503, 2668
MvaI CCWGG 8 cut(s) 1164, 1326, 1503, 1790, 2074, 2356, 2550, 2726
NciI CCSGG 2 cut(s) 360, 666
NcoI CCATGG 1 cut(s) 2340
NheI GCTAGC 1 cut(s) 1471
NlaIII CATG 7 cut(s) 397, 718, 1736, 1940, 2108, 2344, 2708
NmeAIII GCCGAG 1 cut(s) 108
NmuCI GTSAC 3 cut(s) 1728, 2210, 2278
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 2 cut(s) 718, 1736
OliI CACNNNNGTG 1 cut(s) 2219
PagI TCATGA 1 cut(s) 1936
PceI AGGCCT 1 cut(s) 543
PciI ACATGT 1 cut(s) 714
PciSI GCTCTTC 2 cut(s) 1249, 1325
PctI GAATGC 6 cut(s) 221, 1045, 1244, 1946, 2503, 2668
PdmI GAANNNNTTC 2 cut(s) 1082, 2476
PfeI GAWTC 5 cut(s) 367, 613, 700, 1078, 1219
PfoI TCCNGGA 1 cut(s) 1501
PleI GAGTC 3 cut(s) 259, 459, 1303
PpsI GAGTC 3 cut(s) 259, 459, 1303
Ppu21I YACGTR 1 cut(s) 1957
PpuMI RGGWCCY 4 cut(s) 675, 748, 2317, 2358
PscI ACATGT 1 cut(s) 714
PsiI TTATAA 1 cut(s) 495
Psp5II RGGWCCY 4 cut(s) 675, 748, 2317, 2358
Psp6I CCWGG 8 cut(s) 1162, 1324, 1501, 1788, 2072, 2354, 2548, 2724
PspGI CCWGG 8 cut(s) 1162, 1324, 1501, 1788, 2072, 2354, 2548, 2724
PspPI GGNCC 6 cut(s) 675, 748, 2317, 2337, 2358, 2624
PspPPI RGGWCCY 4 cut(s) 675, 748, 2317, 2358
PstI CTGCAG 2 cut(s) 1060, 1483
PstNI CAGNNNCTG 2 cut(s) 1555, 2361
PsuI RGATCY 4 cut(s) 581, 1615, 1644, 2293
PvuII CAGCTG 2 cut(s) 535, 1555
RsaI GTAC 1 cut(s) 2703
RsaNI GTAC 1 cut(s) 2702
RseI CAYNNNNRTG 3 cut(s) 1016, 1965, 2219
SapI GCTCTTC 2 cut(s) 1249, 1325
SaqAI TTAA 7 cut(s) 243, 824, 1515, 1659, 1683, 1892, 2588
Sau96I GGNCC 6 cut(s) 675, 748, 2317, 2337, 2358, 2624
SchI GAGTC 3 cut(s) 259, 460, 1304
SduI GDGCHC 4 cut(s) 69, 1178, 1510, 1962
SexAI ACCWGGT 1 cut(s) 2072
SfaNI GCATC 3 cut(s) 1193, 1525, 1942
SfcI CTRYAG 3 cut(s) 1056, 1479, 1700
SinI GGWCC 5 cut(s) 675, 748, 2317, 2358, 2624
SmiMI CAYNNNNRTG 3 cut(s) 1016, 1965, 2219
SmlI CTYRAG 3 cut(s) 2040, 2380, 2408
SmoI CTYRAG 3 cut(s) 2040, 2380, 2408
SseBI AGGCCT 1 cut(s) 543
SspI AATATT 1 cut(s) 1069
StuI AGGCCT 1 cut(s) 543
StyI CCWWGG 3 cut(s) 607, 751, 2340
TaaI ACNGT 4 cut(s) 1763, 1869, 2094, 2153
TaiI ACGT 3 cut(s) 1467, 1959, 2223
TaqI TCGA 7 cut(s) 268, 280, 365, 656, 1037, 1679, 1823
TatI WGTACW 1 cut(s) 2701
TauI GCSGC 4 cut(s) 94, 128, 1488, 2014
TfiI GAWTC 5 cut(s) 367, 613, 700, 1078, 1219
Tru1I TTAA 7 cut(s) 243, 824, 1515, 1659, 1683, 1892, 2588
Tru9I TTAA 7 cut(s) 243, 824, 1515, 1659, 1683, 1892, 2588
TscAI CASTG 9 cut(s) 108, 424, 807, 903, 1018, 1057, 1766, 2136, 2307
TseFI GTSAC 3 cut(s) 1728, 2210, 2278
Tsp45I GTSAC 3 cut(s) 1728, 2210, 2278
TspDTI ATGAA 4 cut(s) 187, 1953, 2579, 2640
TspGWI ACGGA 1 cut(s) 1678
TspRI CASTG 9 cut(s) 108, 424, 807, 903, 1018, 1057, 1766, 2136, 2307
VneI GTGCAC 1 cut(s) 1958
VpaK11BI GGWCC 5 cut(s) 675, 748, 2317, 2358, 2624
XagI CCTNNNNNAGG 1 cut(s) 2324
XapI RAATTY 4 cut(s) 460, 1574, 1825, 2000
XceI RCATGY 2 cut(s) 718, 1736
XcmI CCANNNNNNNNNTGG 1 cut(s) 2347
XmiI GTMKAC 3 cut(s) 1298, 1794, 1984
XmnI GAANNNNTTC 2 cut(s) 1082, 2476
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.