AT2G17525

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004806
Physical Location & Seq
Forward (+)
7623826 .. 7626186
2361 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G17525.1

Sequence Viewer

Length: 1881 bp
ATGCTGAGAATCTTGAAGCTTTCGACATCTTCTCGATCTGTGCCATTGACAAGATTATGGCTTCTTCAACTGCCTTGTTTCTGCTCATCTTCTTCATCATCGTCTTCTTTGCTTTCTGTGCCCAGCGATGATCATATCGTCCGTCTCATTCTAGACCAGAAATCAGCTTCTGGAGCTTTGGAAACTTTTCGATGGGCTTCGACTTTTCCTGGGTTTATCCATTCTCGTTCCACTTACCGTGCTCTGTTTCATAAACTTTGTGTTTTCCGACGTTTTGATACTGTCTACCAACTGCTCGACGAAATGCCTGACTCAATTGGTTTGCCTCCGGATGATGCTATTTTTGTAACTATCATTAGAGGGTTTGGGCGTGCTCGGTTGATCAAACGTGTGATTAGTGTTGTTGATCTAGTTTCGAAATTTGGGATCAAACCATCTCTGAAAGTTTTCAATTCGATTCTGGATGTTTTGGTTAAAGAAGACATTGACATAGCTAGAGAGTTTTTTACAAGGAAGATGATGGCTAGTGGGATTCATGGTGATGTGTATACATATGGGATTTTGATGAAAGGGCTTTCTTTAACGAATAGGATTGGTGATGGTTTTAAGCTTCTTCAGATCATGAAGACAAGTGGTGTGGCTCCAAACGCTGTGGTTTATAACACTTTGCTTCATGCTCTTTGTAAGAATGGGAAAGTCGGGAGAGCTAGGAGTTTAATGAGTGAAATGAAGGAACCAAATGATGTAACTTTCAACATTTTGATTTCTGCGTACTGCAATGAGCAGAAGTTGATTCAGTCAATGGTGTTGTTGGAGAAATGTTTTAGTTTGGGTTTTGTTCCGGATGTTGTGACGGTAACTAAGGTGATGGAGGTTCTCTGCAATGAAGGCCGTGTGTCTGAGGCACTCGAGGTTTTGGAGAGAGTGGAAAGCAAAGGAGGTAAAGTCGATGTTGTAGCTTGTAACACTTTGGTTAAAGGGTATTGTGCGCTAGGGAAAATGAGAGTTGCTCAACGGTTCTTTATAGAGATGGAGAGAAAAGGTTACTTGCCTAATGTGGAAACATACAACTTGTTGATAGCTGGGTATTGTGATGTTGGAATGTTGGATTCAGCTCTTGACACTTTCAATGATATGAAAACAGATGCGATTAGATGGAACTTTGCTACATTTAACACGTTGATCAGAGGGCTTTCAATAGGGGGGAGGACAGACGATGGTTTAAAGATATTAGAAATGATGCAGGACAGCGATACCGTTCATGGGGCCAGAATCGACCCTTACAATTGTGTTATTTATGGCTTTTACAAGGAAAACCGTTGGGAGGACGCCCTGGAATTCCTCTTAAAGATGGAGAAACTTTTTCCGAGGGCAGTTGACAGGAGCTTCAAGTTGATAAGTTTATGCGAGAAAGGCGGTATGGATGATTTAAAGACGGCCTATGATCAGATGATTGGAGAAGGAGGAGTTCCTAGCATTATAGTATCCCATTGCTTGATTCATAGATACAGCCAACATGGCAAAATAGAAGAATCACTTGAGCTTATTAACGACATGGTGACCAGAGGCTATTTGCCTCGATCATCAACATTCAATGCTGTGATTATCGGGTTCTGTAAGCAAGACAAAGTCATGAATGGTATTAAGTTCGTGGAAGACATGGCTGAAAGAGGTTGCGTTCCTGATACAGAGAGCTACAATCCATTGCTCGAAGAGTTGTGCGTGAAGGGTGATATTCAGAAGGCTTGGTTGCTTTTCTCACGAATGGTGGAGAAGAGTATTGTCCCTGATCCTTCTATGTGGAGTTCACTGATGTTTTGTCTCAGCCAGAAAACCGCAATCCACGTAAACTCCTCATTGCAGGACATAATCCAAAGTTAA

Protein Analysis

626

Amino Acids

70.34

Weight (kDa)

7.78

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 203 - 260 1.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 212 - 244 1.7e-10 PPR repeat
PPR_2 PF13041 215 - 260 1.1e-14 PPR repeat family
PPR PF01535 218 - 245 9.9e-07 PPR repeat
TPR_24 PF23276 247 - 339 5.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 310 - 363 3.1e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 317 - 364 6.4e-15 PPR repeat family
PPR_3 PF13812 343 - 396 6.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 347 - 380 2.2e-09 PPR repeat
PPR_2 PF13041 351 - 398 1.6e-13 PPR repeat family
PPR PF01535 355 - 381 3.4e-06 PPR repeat
PPR_long PF17177 473 - 601 8.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 492 - 540 8.2e-08 PPR repeat family
PPR_2 PF13041 527 - 574 3.7e-09 PPR repeat family
PPR_2 PF13041 561 - 604 3.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 660
AccI GTMKAC 2 cut(s) 285, 548
AccIII TCCGGA 2 cut(s) 328, 841
AciI CCGC 2 cut(s) 1416, 1836
AclWI GGATC 2 cut(s) 434, 1784
AcsI RAATTY 2 cut(s) 419, 1337
AcuI CTGAAG 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 1329
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 2 cut(s) 1263, 1324
AflIII ACRYGT 2 cut(s) 388, 1176
AgsI TTSAA 8 cut(s) 16, 68, 451, 754, 1129, 1197, 1390, 1594
AjnI CCWGG 2 cut(s) 208, 1332
Alw21I GWGCWC 2 cut(s) 244, 376
Alw26I GTCTC 2 cut(s) 149, 1826
AlwI GGATC 2 cut(s) 434, 1784
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 908
Aor13HI TCCGGA 2 cut(s) 328, 841
AoxI GGCC 3 cut(s) 889, 1266, 1437
ApoI RAATTY 2 cut(s) 419, 1337
Asp700I GAANNNNTTC 2 cut(s) 186, 446
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 1 cut(s) 1266
AsuHPI GGTGA 5 cut(s) 551, 608, 877, 1570, 1742
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 908
BaeGI GKGCMC 1 cut(s) 123
BbsI GAAGAC 4 cut(s) 96, 486, 632, 1662
Bbv12I GWGCWC 2 cut(s) 244, 376
BccI CCATC 9 cut(s) 186, 442, 514, 593, 862, 1024, 1149, 1211, 1345
BceAI ACGGC 2 cut(s) 876, 1452
BciT130I CCWGG 2 cut(s) 210, 1334
BciVI GTATCC 1 cut(s) 1495
BclI TGATCA 4 cut(s) 130, 381, 1182, 1444
BcoDI GTCTC 2 cut(s) 149, 1826
BfaI CTAG 7 cut(s) 152, 410, 495, 525, 708, 992, 1473
BfuI GTATCC 1 cut(s) 1495
BglI GCCNNNNNGGC 1 cut(s) 1518
Bme1390I CCNGG 2 cut(s) 210, 1334
BmeT110I CYCGRG 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 1266
BmiI GGNNCC 3 cut(s) 642, 735, 1267
BmrFI CCNGG 2 cut(s) 210, 1334
BmsI GCATC 3 cut(s) 325, 1135, 1230
BpiI GAAGAC 4 cut(s) 96, 486, 632, 1662
BplI GAGNNNNNCTC 2 cut(s) 994, 1026
BpmI CTGGAG 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 1559
BsaAI YACGTR 1 cut(s) 1846
BsaHI GRCGYC 1 cut(s) 1329
BsaJI CCNNGG 3 cut(s) 209, 1332, 1367
BsaWI WCCGGW 2 cut(s) 328, 841
BsaXI ACNNNNNCTCC 4 cut(s) 930, 960, 1835, 1865
Bsc4I CCNNNNNNNGG 2 cut(s) 1263, 1324
Bse3DI GCAATG 5 cut(s) 784, 889, 1489, 1703, 1856
BseAI TCCGGA 2 cut(s) 328, 841
BseBI CCWGG 2 cut(s) 210, 1334
BseDI CCNNGG 3 cut(s) 209, 1332, 1367
BseGI GGATG 4 cut(s) 337, 469, 850, 1429
BseLI CCNNNNNNNGG 2 cut(s) 1263, 1324
BseMI GCAATG 5 cut(s) 784, 889, 1489, 1703, 1856
BseMII CTCAG 2 cut(s) 891, 1837
BseRI GAGGAG 2 cut(s) 1479, 1843
BseSI GKGCMC 1 cut(s) 123
BseYI CCCAGC 2 cut(s) 122, 1082
BshFI GGCC 3 cut(s) 891, 1268, 1439
BsiHKAI GWGCWC 2 cut(s) 244, 376
BsiHKCI CYCGRG 1 cut(s) 908
BsiSI CCGG 2 cut(s) 329, 842
BslFI GGGAC 1 cut(s) 1769
BslI CCNNNNNNNGG 2 cut(s) 1263, 1324
BsmAI GTCTC 2 cut(s) 149, 1826
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 1769
BsnI GGCC 3 cut(s) 891, 1268, 1439
BsoBI CYCGRG 1 cut(s) 908
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 3 cut(s) 123, 244, 376
Bsp13I TCCGGA 2 cut(s) 328, 841
BspACI CCGC 2 cut(s) 1416, 1836
BspANI GGCC 3 cut(s) 891, 1268, 1439
BspCNI CTCAG 2 cut(s) 892, 1836
BspEI TCCGGA 2 cut(s) 328, 841
BspHI TCATGA 2 cut(s) 621, 1632
BspLI GGNNCC 3 cut(s) 642, 735, 1267
BspPI GGATC 2 cut(s) 434, 1784
BspT104I TTCGAA 1 cut(s) 416
BsrDI GCAATG 5 cut(s) 784, 889, 1489, 1703, 1856
BssECI CCNNGG 3 cut(s) 209, 1332, 1367
BssNAI GTATAC 1 cut(s) 549
BssNI GRCGYC 1 cut(s) 1329
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 210, 1334
Bst4CI ACNGT 6 cut(s) 239, 283, 856, 1017, 1258, 1319
Bst6I CTCTTC 2 cut(s) 1707, 1769
BstACI GRCGYC 1 cut(s) 1329
BstBAI YACGTR 1 cut(s) 1846
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 4 cut(s) 5, 861, 900, 1823
BstEII GGTNACC 1 cut(s) 1558
BstF5I GGATG 4 cut(s) 337, 469, 850, 1429
BstHHI GCGC 1 cut(s) 991
BstMAI GTCTC 2 cut(s) 149, 1826
BstMWI GCNNNNNNNGC 6 cut(s) 118, 173, 647, 888, 1413, 1518
BstNI CCWGG 2 cut(s) 210, 1334
BstPI GGTNACC 1 cut(s) 1558
BstSCI CCNGG 2 cut(s) 208, 1332
BstSLI GKGCMC 1 cut(s) 123
BstV2I GAAGAC 4 cut(s) 96, 486, 632, 1662
BstZ17I GTATAC 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1495
BsuRI GGCC 3 cut(s) 891, 1268, 1439
BtgZI GCGATG 1 cut(s) 141
BtsCI GGATG 4 cut(s) 337, 469, 850, 1429
BtsIMutI CAGTG 1 cut(s) 1808
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 2 cut(s) 621, 1632
CfoI GCGC 1 cut(s) 991
Cfr13I GGNCC 1 cut(s) 1266
CseI GACGC 1 cut(s) 1337
Csp6I GTAC 1 cut(s) 772
CspCI CAANNNNNGTGG 4 cut(s) 618, 633, 653, 668
CviAII CATG 8 cut(s) 536, 622, 674, 1262, 1517, 1555, 1633, 1660
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 4 cut(s) 5, 861, 900, 1823
DraI TTTAAA 2 cut(s) 1224, 1431
Eam1104I CTCTTC 2 cut(s) 1707, 1769
EarI CTCTTC 2 cut(s) 1707, 1769
Eco57I CTGAAG 1 cut(s) 599
Eco88I CYCGRG 1 cut(s) 908
Eco91I GGTNACC 1 cut(s) 1558
EcoO65I GGTNACC 1 cut(s) 1558
EcoRI GAATTC 1 cut(s) 1337
EcoRII CCWGG 2 cut(s) 208, 1332
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 8 cut(s) 539, 625, 677, 1265, 1520, 1558, 1636, 1663
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 1 cut(s) 1769
FatI CATG 8 cut(s) 535, 621, 673, 1261, 1516, 1554, 1632, 1659
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 4 cut(s) 130, 381, 1182, 1444
FblI GTMKAC 2 cut(s) 285, 548
FokI GGATG 4 cut(s) 344, 476, 857, 1436
FspBI CTAG 7 cut(s) 152, 410, 495, 525, 708, 992, 1473
GlaI GCGC 1 cut(s) 990
GsaI CCCAGC 2 cut(s) 126, 1086
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 3 cut(s) 891, 1268, 1439
HapII CCGG 2 cut(s) 329, 842
HgaI GACGC 1 cut(s) 1337
HhaI GCGC 1 cut(s) 991
Hin1I GRCGYC 1 cut(s) 1329
Hin1II CATG 8 cut(s) 539, 625, 677, 1265, 1520, 1558, 1636, 1663
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HindIII AAGCTT 2 cut(s) 17, 608
HinfI GANTC 9 cut(s) 9, 311, 457, 532, 793, 1109, 1272, 1498, 1532
HpaII CCGG 2 cut(s) 329, 842
HphI GGTGA 5 cut(s) 551, 608, 877, 1570, 1742
Hpy166II GTNNAC 5 cut(s) 286, 549, 1378, 1808, 1849
Hpy188I TCNGA 8 cut(s) 269, 441, 618, 901, 1187, 1368, 1449, 1740
Hpy8I GTNNAC 5 cut(s) 286, 549, 1378, 1808, 1849
Hpy99I CGWCG 2 cut(s) 273, 302
HpyAV CCTTC 6 cut(s) 724, 881, 1454, 1720, 1735, 1803
HpyCH4III ACNGT 6 cut(s) 239, 283, 856, 1017, 1258, 1319
HpyCH4IV ACGT 4 cut(s) 271, 388, 1178, 1845
HpyCH4V TGCA 4 cut(s) 777, 882, 1243, 1861
HpyF10VI GCNNNNNNNGC 6 cut(s) 118, 173, 647, 888, 1413, 1518
HpyF3I CTNAG 4 cut(s) 5, 861, 900, 1823
HpySE526I ACGT 4 cut(s) 271, 388, 1178, 1845
Hsp92I GRCGYC 1 cut(s) 1329
Hsp92II CATG 8 cut(s) 539, 625, 677, 1265, 1520, 1558, 1636, 1663
HspAI GCGC 1 cut(s) 989
Kpn2I TCCGGA 2 cut(s) 328, 841
Ksp22I TGATCA 4 cut(s) 130, 381, 1182, 1444
LmnI GCTCC 3 cut(s) 173, 646, 1383
LweI GCATC 3 cut(s) 325, 1135, 1230
MaeI CTAG 7 cut(s) 152, 410, 495, 525, 708, 992, 1473
MaeII ACGT 4 cut(s) 271, 388, 1178, 1845
MaeIII GTNAC 7 cut(s) 346, 745, 850, 856, 962, 1043, 1558
MfeI CAATTG 2 cut(s) 315, 1285
MhlI GDGCHC 3 cut(s) 123, 244, 376
MluCI AATT 5 cut(s) 315, 419, 451, 1285, 1337
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 4 cut(s) 292, 792, 1078, 1086
MroI TCCGGA 2 cut(s) 328, 841
MroXI GAANNNNTTC 2 cut(s) 186, 446
MslI CAYNNNNRTG 1 cut(s) 540
MspI CCGG 2 cut(s) 329, 842
MspR9I CCNGG 2 cut(s) 210, 1334
MunI CAATTG 2 cut(s) 315, 1285
MvaI CCWGG 2 cut(s) 210, 1334
MwoI GCNNNNNNNGC 6 cut(s) 118, 173, 647, 888, 1413, 1518
NdeI CATATG 1 cut(s) 553
NlaIII CATG 8 cut(s) 539, 625, 677, 1265, 1520, 1558, 1636, 1663
NlaIV GGNNCC 3 cut(s) 642, 735, 1267
NmuCI GTSAC 2 cut(s) 850, 1558
NspV TTCGAA 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 908
PagI TCATGA 2 cut(s) 621, 1632
PdmI GAANNNNTTC 2 cut(s) 186, 446
PfeI GAWTC 8 cut(s) 9, 457, 532, 793, 1109, 1272, 1498, 1532
PflFI GACNNNGTC 1 cut(s) 1628
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
Ppu21I YACGTR 1 cut(s) 1846
PsiI TTATAA 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 208, 1332
PspEI GGTNACC 1 cut(s) 1558
PspFI CCCAGC 2 cut(s) 122, 1082
PspGI CCWGG 2 cut(s) 208, 1332
PspN4I GGNNCC 3 cut(s) 642, 735, 1267
PspPI GGNCC 1 cut(s) 1266
PspXI VCTCGAGB 1 cut(s) 908
PstNI CAGNNNCTG 1 cut(s) 170
PsyI GACNNNGTC 1 cut(s) 1628
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 1 cut(s) 540
Sau96I GGNCC 1 cut(s) 1266
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 210, 1334
SduI GDGCHC 3 cut(s) 123, 244, 376
SfaNI GCATC 3 cut(s) 325, 1135, 1230
Sfr274I CTCGAG 1 cut(s) 908
SfuI TTCGAA 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 2 cut(s) 908, 1538
SmoI CTYRAG 2 cut(s) 908, 1538
Sse9I AATT 5 cut(s) 315, 419, 451, 1285, 1337
SsiI CCGC 2 cut(s) 1416, 1836
SspMI CTAG 7 cut(s) 152, 410, 495, 525, 708, 992, 1473
StyD4I CCNGG 2 cut(s) 208, 1332
TaaI ACNGT 6 cut(s) 239, 283, 856, 1017, 1258, 1319
TaiI ACGT 4 cut(s) 274, 391, 1181, 1848
TasI AATT 5 cut(s) 315, 419, 451, 1285, 1337
TfiI GAWTC 8 cut(s) 9, 457, 532, 793, 1109, 1272, 1498, 1532
TscAI CASTG 1 cut(s) 1815
TseFI GTSAC 2 cut(s) 850, 1558
Tsp45I GTSAC 2 cut(s) 850, 1558
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 1 cut(s) 1815
Tth111I GACNNNGTC 1 cut(s) 1628
XapI RAATTY 2 cut(s) 419, 1337
XbaI TCTAGA 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 908
XmiI GTMKAC 2 cut(s) 285, 548
XmnI GAANNNNTTC 2 cut(s) 186, 446
XspI CTAG 7 cut(s) 152, 410, 495, 525, 708, 992, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.