FvH4_7g03710

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004806
Physical Location & Seq
Forward (+)
4228064 .. 4230825
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03710.t1

Sequence Viewer

Length: 1839 bp
ATGCTAATCTCAAAGCTTCCGCATGTTCTCCGACGAACTCTGTCCTCCATCTCTGTTCCATCTCATACCCACATTGCCCATCTTCTGCTAGAACAAAAATCAGCCACCCAGGTCCTCGACACCTTCCAATGGGCCTCCACACTCCCCAGCTTCACCCACTCTCCGTCCACTTACCGAGCACTCATCCACAAGCTATGTACCTTTCGCCGCTTCGACACTGCCTACCAAGTGCTCGACGAAATGCCCCGCTCAATTGGCCAACCCCCAGATGAGCACATTTTCGTCACGCTCATTCACGGCCTCGGCCGAGCCCATTTGGTAAAGCAAGCCATCAAGGTGCTCGACTTGGTTGCAAGGTATAACCAGAAACCTTCTCTTAAGATTTTCAACTCGATACTCGATGTGCTTGTGCAGGAAGATATTGATATAGCTAGGGAGTTTTATAGGAAGAAGATGATGGCGAGTGGGGTCAAGGGTGATGATTATACTTTTGGTATCTTGATGAAGGGTCTTTGCTTGACAAGTAGGATTGGTGATGGTTTTAAGCTCTTGCAAGCCATTAAGTCGAGGGGAGTTGCGCCGAATACTGTGGTGTATAACACATTGCTGCATGCTCTTTGCAGGAATGGGAAAGTTGGGAGAGCCAGAAGCTTGATGAATGAGATGGAAGACCCTAATGATGTGACTTTTAATATTTTGATATCTGGTTATTGCGGAGAAGAGAATTTGGTGCAGGCGGTTGTTATGTTGGACAAGTGCTTTGGTCTCGGGTATGTGCCTGATGTTGTCACCATAACCAAGGTGTTGGAACTTCTGTGCAATGATGGTCGGGTGATGGAGGCTGTCAAGGTTTTAGAGAGAGTTGAGAGCAATGGAGGGTTGGTTGATGTTGTGGCATATAATACCTTGATTAAGGGTTTCTGTCAATTAGGAAAAGCGAAACTTGGTCTTCGCATTTTGAAGGAGATGGAGAGAAAGGGATGCCTTCCAGATGTTGATACCTACAATGTCTTGATCTCGGGTTTTTGTGAGTCTGGGATGTTGGACAAAGCTCTTGATCTGTTTAATGATATGAAAACAGATGGAATCAATTGGAATTTTGCTACATACGATGTGTTAATAAGAGGCTTGTGTTCAGGAGGAAGAACTGAAGATGGGTTAAAAATTTTGGATTTGATGAATGAGAGCAAAGATGGTTGTAGGGGTCGAATAAGTCCTTTTAATAGTGTGCTATATGGCCTGTATAAGGAAAACCGTTTGGATGAAGCACTTGAGTTTCTTGCCAGTTTGGGTAAACTATTTCCCAGAGCTGTTGATAGGAGCTTGAGGATTTTAGGTTTCTGTGAGGAGGGTGCCACAGAGAATGCAAAGAAGCTCTATGATCAGATGATTCTGGAAAGGGCAGTCCCATGTGTTTTGATTTATGACTATCTAATCCATAGATTTTGCCAACAAGGGTGTGTTCGTCAAGCCTTTGAGCTTATGAATGAGATGATTGTTCATTGTTACTTCCCTGTTGCATCGACTTTCAATGTTCTGATTAATGGATTTTGCAAGCAAGGAAAAGTTGATAGTGCGCTAAAACTCCTCGAAGAAATGGTTGGTAGAGGTTGTTTACCAGATATAGACAGTTACAGTCATTTGGTAGGTGTCCTTTGCCAGAAGGGGGATTTTCAGAAAGCTTTAACTCTCCTTTTCCAAATGGTCGAAAAGGGTATCACTCCTGATCATTTCACATGGAACTCGTTGCTTGTTTGTACAAGTCTAGAGACTGTATGGTTAAACAGTAAAAGTATGCTTTATGCGAACAATCAGTTAGATTGTATTATTAAGGCTTAA

Protein Analysis

613

Amino Acids

68.66

Weight (kDa)

7.46

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 182 - 238 6.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 190 - 223 2.2e-11 PPR repeat
PPR_2 PF13041 194 - 238 1.8e-14 PPR repeat family
PPR PF01535 197 - 223 2.5e-06 PPR repeat
PPR_1 PF12854 225 - 252 1.5e-06 PPR repeat
PPR_3 PF13812 285 - 341 6.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 293 - 324 7.6e-07 PPR repeat
PPR_2 PF13041 296 - 343 6.8e-18 PPR repeat family
PPR PF01535 298 - 328 1.2e-07 PPR repeat
PPR_1 PF12854 326 - 359 8.5e-14 PPR repeat
PPR_2 PF13041 330 - 378 3.9e-17 PPR repeat family
PPR PF01535 334 - 363 5.3e-10 PPR repeat
PPR_long PF17177 455 - 584 2e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 503 - 534 7.9e-12 PPR repeat
PPR_2 PF13041 507 - 553 3.2e-14 PPR repeat family
PPR PF01535 508 - 538 8.1e-10 PPR repeat
PPR_3 PF13812 528 - 584 3.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 540 - 584 2.9e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1352
AccBSI CCGCTC 1 cut(s) 249
AciI CCGC 5 cut(s) 20, 208, 247, 714, 737
AcoI YGGCCR 2 cut(s) 256, 304
AcsI RAATTY 3 cut(s) 724, 1096, 1164
AcuI CTGAAG 1 cut(s) 1170
AfaI GTAC 2 cut(s) 199, 1759
AfiI CCNNNNNNNGG 3 cut(s) 129, 1246, 1666
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 3 cut(s) 388, 961, 1531
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 1584, 1616
Alw21I GWGCWC 4 cut(s) 181, 234, 276, 342
Alw26I GTCTC 2 cut(s) 770, 1763
Ama87I CYCGRG 2 cut(s) 767, 1018
AoxI GGCC 5 cut(s) 132, 256, 298, 304, 1237
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 3 cut(s) 724, 1096, 1164
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AseI ATTAAT 1 cut(s) 1542
AspLEI GCGC 2 cut(s) 580, 1579
AspS9I GGNCC 2 cut(s) 112, 132
AsuHPI GGTGA 5 cut(s) 145, 488, 545, 781, 844
AvaI CYCGRG 2 cut(s) 767, 1018
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 1 cut(s) 1352
BanII GRGCYC 1 cut(s) 313
BbsI GAAGAC 2 cut(s) 675, 941
Bbv12I GWGCWC 4 cut(s) 181, 234, 276, 342
BbvI GCAGC 1 cut(s) 594
BceAI ACGGC 1 cut(s) 313
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 1 cut(s) 110
BclI TGATCA 2 cut(s) 1381, 1726
BcoDI GTCTC 2 cut(s) 770, 1763
BfaI CTAG 3 cut(s) 89, 432, 1766
BfrI CTTAAG 1 cut(s) 377
BisI GCNGC 2 cut(s) 208, 608
BlsI GCNGC 2 cut(s) 209, 609
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 2 cut(s) 767, 1018
BmgT120I GGNCC 2 cut(s) 112, 132
BmiI GGNNCC 1 cut(s) 1354
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 2 cut(s) 971, 1529
BpiI GAAGAC 2 cut(s) 675, 941
BpuEI CTTGAG 2 cut(s) 1292, 1345
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 3 cut(s) 108, 301, 798
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 867, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 1246, 1666
Bse1I ACTGG 1 cut(s) 1284
Bse3DI GCAATG 4 cut(s) 72, 602, 826, 877
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 108, 301, 798
BseGI GGATG 4 cut(s) 183, 986, 1044, 1267
BseLI CCNNNNNNNGG 3 cut(s) 129, 1246, 1666
BseMI GCAATG 4 cut(s) 72, 602, 826, 877
BseNI ACTGG 1 cut(s) 1284
BseRI GAGGAG 2 cut(s) 1361, 1577
BseX3I CGGCCG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 594
BseYI CCCAGC 1 cut(s) 146
BsgI GTGCAG 2 cut(s) 431, 752
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 5 cut(s) 134, 258, 300, 306, 1239
BshNI GGYRCC 1 cut(s) 1352
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 4 cut(s) 181, 234, 276, 342
BsiHKCI CYCGRG 2 cut(s) 767, 1018
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 3 cut(s) 129, 1246, 1666
BsmAI GTCTC 2 cut(s) 770, 1763
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 1369
BsnI GGCC 5 cut(s) 134, 258, 300, 306, 1239
Bso31I GGTCTC 1 cut(s) 770
BsoBI CYCGRG 2 cut(s) 767, 1018
Bsp1286I GDGCHC 5 cut(s) 181, 234, 276, 313, 342
Bsp1407I TGTACA 1 cut(s) 1757
Bsp143I GATC 4 cut(s) 1014, 1057, 1381, 1726
BspACI CCGC 5 cut(s) 20, 208, 247, 714, 737
BspANI GGCC 5 cut(s) 134, 258, 300, 306, 1239
BspLI GGNNCC 1 cut(s) 1354
BspT107I GGYRCC 1 cut(s) 1352
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 4 cut(s) 72, 602, 826, 877
BsrGI TGTACA 1 cut(s) 1757
BsrI ACTGG 1 cut(s) 1284
BssECI CCNNGG 3 cut(s) 108, 301, 798
BssMI GATC 4 cut(s) 1014, 1057, 1381, 1726
BssT1I CCWWGG 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 6 cut(s) 589, 1256, 1631, 1637, 1774, 1787
Bst6I CTCTTC 1 cut(s) 714
BstAFI CTTAAG 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 1757
BstC8I GCNNGC 5 cut(s) 327, 555, 612, 735, 1556
BstENI CCTNNNNNAGG 1 cut(s) 1244
BstF5I GGATG 4 cut(s) 183, 986, 1044, 1267
BstHHI GCGC 2 cut(s) 580, 1579
BstKTI GATC 4 cut(s) 1017, 1060, 1384, 1729
BstMAI GTCTC 2 cut(s) 770, 1763
BstMBI GATC 4 cut(s) 1014, 1057, 1381, 1726
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 2 cut(s) 26, 614
BstSCI CCNGG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 594
BstV2I GAAGAC 2 cut(s) 675, 941
BstXI CCANNNNNNTGG 1 cut(s) 805
BstZI CGGCCG 1 cut(s) 304
BsuRI GGCC 5 cut(s) 134, 258, 300, 306, 1239
BtsCI GGATG 4 cut(s) 183, 986, 1044, 1267
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 5 cut(s) 327, 555, 612, 735, 1556
CfoI GCGC 2 cut(s) 580, 1579
Cfr13I GGNCC 2 cut(s) 112, 132
Csp6I GTAC 2 cut(s) 198, 1758
CviAII CATG 4 cut(s) 23, 611, 1410, 1737
CviQI GTAC 2 cut(s) 198, 1758
DpnI GATC 4 cut(s) 1016, 1059, 1383, 1728
DpnII GATC 4 cut(s) 1014, 1057, 1381, 1726
EaeI YGGCCR 2 cut(s) 256, 304
EagI CGGCCG 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
EclXI CGGCCG 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 313
Eco31I GGTCTC 1 cut(s) 770
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 1170
Eco88I CYCGRG 2 cut(s) 767, 1018
EcoNI CCTNNNNNAGG 1 cut(s) 1244
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 108
EcoRV GATATC 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 798
EcoT38I GRGCYC 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 798
FaeI CATG 4 cut(s) 26, 614, 1413, 1740
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 1 cut(s) 1391
FatI CATG 4 cut(s) 22, 610, 1409, 1736
FauI CCCGC 1 cut(s) 254
FbaI TGATCA 2 cut(s) 1381, 1726
Fnu4HI GCNGC 2 cut(s) 208, 608
FokI GGATG 4 cut(s) 170, 993, 1051, 1274
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 208, 608
FspBI CTAG 3 cut(s) 89, 432, 1766
GlaI GCGC 2 cut(s) 579, 1578
GluI GCNGC 2 cut(s) 208, 608
GsaI CCCAGC 1 cut(s) 150
HaeIII GGCC 5 cut(s) 134, 258, 300, 306, 1239
HhaI GCGC 2 cut(s) 580, 1579
Hin1II CATG 4 cut(s) 26, 614, 1413, 1740
Hin6I GCGC 2 cut(s) 578, 1577
HinP1I GCGC 2 cut(s) 578, 1577
HindIII AAGCTT 3 cut(s) 14, 649, 1680
HinfI GANTC 3 cut(s) 1031, 1086, 1390
HphI GGTGA 5 cut(s) 145, 488, 545, 781, 844
Hpy166II GTNNAC 3 cut(s) 168, 1295, 1616
Hpy188I TCNGA 4 cut(s) 32, 1386, 1539, 1677
Hpy188III TCNNGA 8 cut(s) 499, 989, 1012, 1055, 1137, 1394, 1724, 1766
Hpy8I GTNNAC 3 cut(s) 168, 1295, 1616
Hpy99I CGWCG 2 cut(s) 36, 239
HpyAV CCTTC 6 cut(s) 133, 381, 499, 955, 995, 1657
HpyCH4III ACNGT 6 cut(s) 589, 1256, 1631, 1637, 1774, 1787
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
Hsp92II CATG 4 cut(s) 26, 614, 1413, 1740
HspAI GCGC 2 cut(s) 578, 1577
Ksp22I TGATCA 2 cut(s) 1381, 1726
Kzo9I GATC 4 cut(s) 1014, 1057, 1381, 1726
LmnI GCTCC 1 cut(s) 1320
Lsp1109I GCAGC 1 cut(s) 594
LweI GCATC 2 cut(s) 971, 1529
MaeI CTAG 3 cut(s) 89, 432, 1766
MaeIII GTNAC 5 cut(s) 283, 682, 787, 1505, 1631
MalI GATC 4 cut(s) 1016, 1059, 1383, 1728
MbiI CCGCTC 1 cut(s) 249
MboI GATC 4 cut(s) 1014, 1057, 1381, 1726
MfeI CAATTG 2 cut(s) 252, 1090
MhlI GDGCHC 5 cut(s) 181, 234, 276, 313, 342
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 6 cut(s) 252, 724, 926, 1090, 1096, 1164
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 1040
MmeI TCCRAC 4 cut(s) 55, 729, 786, 1023
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 258
MspCI CTTAAG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 2 cut(s) 252, 1090
Mva1269I GAATGC 1 cut(s) 1369
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 255
NdeII GATC 4 cut(s) 1014, 1057, 1381, 1726
NlaIII CATG 4 cut(s) 26, 614, 1413, 1740
NlaIV GGNNCC 1 cut(s) 1354
NmeAIII GCCGAG 2 cut(s) 282, 332
NmuCI GTSAC 3 cut(s) 283, 682, 787
NspI RCATGY 2 cut(s) 26, 614
PaeI GCATGC 1 cut(s) 614
PctI GAATGC 1 cut(s) 1369
PfeI GAWTC 2 cut(s) 1086, 1390
PkrI GCNGC 2 cut(s) 209, 609
PleI GAGTC 1 cut(s) 1039
PpsI GAGTC 1 cut(s) 1039
PpuMI RGGWCCY 1 cut(s) 112
PshBI ATTAAT 1 cut(s) 1542
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 108
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 1354
PspPI GGNCC 2 cut(s) 112, 132
PspPPI RGGWCCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 199, 1759
RsaNI GTAC 2 cut(s) 198, 1758
RseI CAYNNNNRTG 1 cut(s) 335
SatI GCNGC 2 cut(s) 208, 608
Sau3AI GATC 4 cut(s) 1014, 1057, 1381, 1726
Sau96I GGNCC 2 cut(s) 112, 132
SchI GAGTC 1 cut(s) 1040
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 5 cut(s) 181, 234, 276, 313, 342
SfaNI GCATC 2 cut(s) 971, 1529
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 3 cut(s) 377, 1271, 1324
SmoI CTYRAG 3 cut(s) 377, 1271, 1324
SphI GCATGC 1 cut(s) 614
Sse9I AATT 6 cut(s) 252, 724, 926, 1090, 1096, 1164
SsiI CCGC 5 cut(s) 20, 208, 247, 714, 737
SspI AATATT 1 cut(s) 694
SspMI CTAG 3 cut(s) 89, 432, 1766
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 798
TaaI ACNGT 6 cut(s) 589, 1256, 1631, 1637, 1774, 1787
TasI AATT 6 cut(s) 252, 724, 926, 1090, 1096, 1164
TatI WGTACW 1 cut(s) 1757
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 2 cut(s) 1086, 1390
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 3 cut(s) 283, 682, 787
TseI GCWGC 1 cut(s) 607
Tsp45I GTSAC 3 cut(s) 283, 682, 787
TspDTI ATGAA 7 cut(s) 518, 671, 1088, 1193, 1278, 1490, 1499
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 223
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 112
VspI ATTAAT 1 cut(s) 1542
XagI CCTNNNNNAGG 1 cut(s) 1244
XapI RAATTY 3 cut(s) 724, 1096, 1164
XbaI TCTAGA 1 cut(s) 1765
XceI RCATGY 2 cut(s) 26, 614
XspI CTAG 3 cut(s) 89, 432, 1766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.