pycom10g02880

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004806
Physical Location & Seq
Reverse (-)
3216783 .. 3217787
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02880.1

Sequence Viewer

Length: 1005 bp
ATGGAGGCTCTCAAGCTTATTGAGAGAGTGGAGAGCAAGGGAGGACTGGTGGATGTGGTAGCTTATAACACCTTGGTTAAGGGTTTCTGTAGATTAGGCGAAGCGAAACTCAGTCTTCGCATTGTGGAGGAAATGGAGAAAAAGGGACGCCTTCCAAATGTTGATACTTACAATGTCTTGATCTCTTGTTTTTGCGAGTCTGGGATGTCGGATATGGCTCTGGATCTGTTTAAGGATATGGAAACATATGGTATCTACTGGAATTTTGTTACGTATGATAGGTTAATTAGAGGTTTTTGTGTAAGAGGAAGGACGAAGAAAGGGTTGGAAATTTTGGAACTAATGAATGAGAGAAAACACGGTTCTGGAGGCCAAATTTCTCCTTATAATAGTGTGTTATATGGGCTGTATAAGGAAAATCGTTTAGGCGAAGCACTTGAGTTCTTAACCAGTATGGGGAAACTATTTCCCAGAGCTGTCGATAGAAGCTTGAAAATTTTAGGTTTCTGTGAGGAGGGCGCCACATTGAATGCGAAGAAGATTTATGATCAGATGATTACAGAAAACGGAGTTCCAAGTGCTATATTCTTTCACTGTCTAATCCATGGATTTTGCCAAGAAGGCTCTGTTCGCGAAGCTTTTGACCTAATGAATGAGATGATTGGTCATGGTTACTTTCCTCTTGCATCAACGTATAATCCTGTGATCAATGCGTTTTGTGAGAAAGTGAAAGTTGGCAGTGCACTGAAACTTGTGGAGGACATGGCCGAGCGAGGTTGCTCACCCGATAGCGGAAGTTATAGTCCGTTGGTAGCTGTTCTTTGCCGGATGGGGGATTTTCAGAAAGATTTGAGACTGGTTTTGCAGTCGGTAGAAAAGGGCATCATTCCAAATTTCCTGACATGGAACTCGTTGCTTGTTTGTTTAAGTCAAGAGACTGTATGGTTGAAGGGAAAAAGTATAATCGATGTAAATAACAAGTTACACTATATTATTGAGAATTAG

Protein Analysis

335

Amino Acids

37.59

Weight (kDa)

6.27

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 6 - 64 4.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 14 - 46 5.4e-07 PPR repeat
PPR_2 PF13041 18 - 65 5.2e-15 PPR repeat family
PPR_1 PF12854 48 - 81 1e-08 PPR repeat
PPR_2 PF13041 52 - 100 1.3e-13 PPR repeat family
PPR PF01535 56 - 85 1e-08 PPR repeat
PPR_long PF17177 174 - 282 6.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 191 - 220 2.9e-08 PPR repeat
PPR_2 PF13041 192 - 227 3.3e-06 PPR repeat family
PPR PF01535 197 - 224 4e-06 PPR repeat
PPR_2 PF13041 230 - 276 5.2e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 66, 387
AccB1I GGYRCC 1 cut(s) 518
AccII CGCG 1 cut(s) 633
AciI CCGC 1 cut(s) 792
AclWI GGATC 1 cut(s) 231
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 5 cut(s) 262, 330, 375, 495, 892
AcyI GRCGYC 2 cut(s) 148, 519
AfiI CCNNNNNNNGG 3 cut(s) 456, 791, 832
AgsI TTSAA 3 cut(s) 493, 529, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 6 cut(s) 16, 62, 476, 489, 638, 815
AluI AGCT 6 cut(s) 16, 62, 476, 489, 638, 815
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 2 cut(s) 847, 929
Alw44I GTGCAC 1 cut(s) 741
AlwI GGATC 1 cut(s) 231
AoxI GGCC 2 cut(s) 370, 765
ApaLI GTGCAC 1 cut(s) 741
ApoI RAATTY 5 cut(s) 262, 330, 375, 495, 892
AspLEI GCGC 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 745
BccI CCATC 1 cut(s) 823
BclI TGATCA 2 cut(s) 547, 705
BcoDI GTCTC 2 cut(s) 847, 929
BfmI CTRYAG 1 cut(s) 88
BfoI RGCGCY 1 cut(s) 522
BglI GCCNNNNNGGC 1 cut(s) 621
BmiI GGNNCC 1 cut(s) 520
BmsI GCATC 2 cut(s) 695, 891
BpiI GAAGAC 1 cut(s) 107
BpmI CTGGAG 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 966
BsaAI YACGTR 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 148, 519
BsaJI CCNNGG 2 cut(s) 72, 604
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 456, 791, 832
Bse1I ACTGG 4 cut(s) 51, 263, 450, 861
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 2 cut(s) 72, 604
BseGI GGATG 3 cut(s) 58, 210, 834
BseLI CCNNNNNNNGG 3 cut(s) 456, 791, 832
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 4 cut(s) 51, 263, 450, 861
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 745
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 2 cut(s) 372, 767
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 966
BsiHKAI GWGCWC 1 cut(s) 745
BsiSI CCGG 1 cut(s) 826
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 3 cut(s) 456, 791, 832
BsmAI GTCTC 2 cut(s) 847, 929
BsmFI GGGAC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 2 cut(s) 372, 767
Bsp1286I GDGCHC 1 cut(s) 745
Bsp143I GATC 4 cut(s) 180, 223, 547, 705
Bsp19I CCATGG 1 cut(s) 604
Bsp68I TCGCGA 1 cut(s) 633
BspACI CCGC 1 cut(s) 792
BspANI GGCC 2 cut(s) 372, 767
BspCNI CTCAG 1 cut(s) 123
BspDI ATCGAT 1 cut(s) 966
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 520
BspPI GGATC 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 518
BsrI ACTGG 4 cut(s) 51, 263, 450, 861
BssECI CCNNGG 2 cut(s) 72, 604
BssMI GATC 4 cut(s) 180, 223, 547, 705
BssNI GRCGYC 2 cut(s) 148, 519
BssT1I CCWWGG 2 cut(s) 72, 604
Bst4CI ACNGT 3 cut(s) 362, 596, 940
BstACI GRCGYC 2 cut(s) 148, 519
BstBAI YACGTR 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 3 cut(s) 58, 210, 834
BstFNI CGCG 1 cut(s) 633
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 4 cut(s) 183, 226, 550, 708
BstMAI GTCTC 2 cut(s) 847, 929
BstMBI GATC 4 cut(s) 180, 223, 547, 705
BstMWI GCNNNNNNNGC 2 cut(s) 621, 630
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 745
BstSNI TACGTA 1 cut(s) 273
BstUI CGCG 1 cut(s) 633
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 2 cut(s) 372, 767
BsuTUI ATCGAT 1 cut(s) 966
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 3 cut(s) 58, 210, 834
BtsI GCAGTG 1 cut(s) 745
BtsIMutI CAGTG 3 cut(s) 592, 743, 745
BtuMI TCGCGA 1 cut(s) 633
CfoI GCGC 1 cut(s) 521
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 1 cut(s) 156
CviAII CATG 4 cut(s) 605, 668, 763, 903
DdeI CTNAG 1 cut(s) 110
DinI GGCGCC 1 cut(s) 520
DpnI GATC 4 cut(s) 182, 225, 549, 707
DpnII GATC 4 cut(s) 180, 223, 547, 705
EaeI YGGCCR 1 cut(s) 765
Eco105I TACGTA 1 cut(s) 273
Eco130I CCWWGG 2 cut(s) 72, 604
EcoT14I CCWWGG 2 cut(s) 72, 604
EgeI GGCGCC 1 cut(s) 520
EheI GGCGCC 1 cut(s) 520
ErhI CCWWGG 2 cut(s) 72, 604
FaeI CATG 4 cut(s) 608, 671, 766, 906
FaqI GGGAC 1 cut(s) 159
FatI CATG 4 cut(s) 604, 667, 762, 902
FauNDI CATATG 1 cut(s) 247
FbaI TGATCA 2 cut(s) 547, 705
FokI GGATG 3 cut(s) 65, 217, 841
GlaI GCGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 2 cut(s) 372, 767
HapII CCGG 1 cut(s) 826
HgaI GACGC 1 cut(s) 156
HhaI GCGC 1 cut(s) 521
Hin1I GRCGYC 2 cut(s) 148, 519
Hin1II CATG 4 cut(s) 608, 671, 766, 906
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HindIII AAGCTT 3 cut(s) 14, 487, 636
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 826
HphI GGTGA 1 cut(s) 774
Hpy166II GTNNAC 1 cut(s) 743
Hpy188I TCNGA 3 cut(s) 211, 552, 843
Hpy188III TCNNGA 6 cut(s) 178, 221, 366, 632, 898, 932
Hpy8I GTNNAC 1 cut(s) 743
HpyAV CCTTC 4 cut(s) 161, 303, 614, 943
HpyCH4III ACNGT 3 cut(s) 362, 596, 940
HpyCH4IV ACGT 2 cut(s) 272, 692
HpyCH4V TGCA 3 cut(s) 686, 743, 865
HpyF10VI GCNNNNNNNGC 2 cut(s) 621, 630
HpyF3I CTNAG 1 cut(s) 110
HpySE526I ACGT 2 cut(s) 272, 692
Hsp92I GRCGYC 2 cut(s) 148, 519
Hsp92II CATG 4 cut(s) 608, 671, 766, 906
HspAI GCGC 1 cut(s) 519
KasI GGCGCC 1 cut(s) 518
Ksp22I TGATCA 2 cut(s) 547, 705
Kzo9I GATC 4 cut(s) 180, 223, 547, 705
LweI GCATC 2 cut(s) 695, 891
MaeII ACGT 2 cut(s) 272, 692
MaeIII GTNAC 3 cut(s) 268, 671, 981
MalI GATC 4 cut(s) 182, 225, 549, 707
MboI GATC 4 cut(s) 180, 223, 547, 705
MboII GAAGA 4 cut(s) 107, 328, 547, 550
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 7 cut(s) 262, 285, 330, 375, 495, 892, 1000
Mly113I GGCGCC 1 cut(s) 519
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 189, 306
MseI TTAA 5 cut(s) 78, 231, 284, 446, 926
MspI CCGG 1 cut(s) 826
Mva1269I GAATGC 1 cut(s) 535
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 2 cut(s) 621, 630
NarI GGCGCC 1 cut(s) 519
NcoI CCATGG 1 cut(s) 604
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 180, 223, 547, 705
NlaIII CATG 4 cut(s) 608, 671, 766, 906
NlaIV GGNNCC 1 cut(s) 520
NmeAIII GCCGAG 1 cut(s) 793
NruI TCGCGA 1 cut(s) 633
PctI GAATGC 1 cut(s) 535
PleI GAGTC 1 cut(s) 205
PluTI GGCGCC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 205
Ppu21I YACGTR 1 cut(s) 273
PsiI TTATAA 2 cut(s) 66, 387
PspN4I GGNNCC 1 cut(s) 520
PsuI RGATCY 1 cut(s) 223
RruI TCGCGA 1 cut(s) 633
SaqAI TTAA 5 cut(s) 78, 231, 284, 446, 926
Sau3AI GATC 4 cut(s) 180, 223, 547, 705
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 2 cut(s) 695, 891
SfcI CTRYAG 1 cut(s) 88
SfoI GGCGCC 1 cut(s) 520
SmlI CTYRAG 2 cut(s) 11, 437
SmoI CTYRAG 2 cut(s) 11, 437
SnaBI TACGTA 1 cut(s) 273
Sse9I AATT 7 cut(s) 262, 285, 330, 375, 495, 892, 1000
SsiI CCGC 1 cut(s) 792
SspDI GGCGCC 1 cut(s) 518
StyI CCWWGG 2 cut(s) 72, 604
TaaI ACNGT 3 cut(s) 362, 596, 940
TaiI ACGT 2 cut(s) 275, 695
TaqI TCGA 2 cut(s) 480, 966
TasI AATT 7 cut(s) 262, 285, 330, 375, 495, 892, 1000
Tru1I TTAA 5 cut(s) 78, 231, 284, 446, 926
Tru9I TTAA 5 cut(s) 78, 231, 284, 446, 926
TscAI CASTG 3 cut(s) 599, 745, 750
TspDTI ATGAA 2 cut(s) 359, 665
TspGWI ACGGA 2 cut(s) 582, 795
TspRI CASTG 3 cut(s) 599, 745, 750
VneI GTGCAC 1 cut(s) 741
XapI RAATTY 5 cut(s) 262, 330, 375, 495, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.